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1. 4 The incubator wil begin cooling or heating to achieve the new Daytime set point Ena of proce E OPERATION CONTINUED SET THE NIGHTTIME TEMPERATURE SET POINT Perform the steps below to adiust the temperature set point of the Nightime mode to tha of your iboratory or study protocol The incubator comes frm ne factory with a Night set point of 20 C Skip this procedure if your protocol requires incubator to be run at 20 C under right condfions wah the chamber dark Matching fo set point 1o your protocol also prepares the uit for temperature calibraton I auto cycle is active and you wil be performing a temperature calibration aar adjusting the set pins terminate the The unit must be run in Nighttime mode adjust he Night time mode Advance to step 2 2 Program Active Light Of 2 the auto cycle is running in Daytime mode terminate the o and manually place the und in Nightime modo Press and hold the Cycle Start button for two seconds The Program Active Light wil extinguish b To switch from Daytime to Night press and hold the Mode button or 5 second The Day Temp indicator wil extinguish the blue Night Temp vil iluminate Place the Temperatura display io the Nighttime Sat Point mode Press and release the Up or the Down arrow button b The Time display wil go dark The Temperature display wil briefly fash the letters SP 4 The display wil
2. channa based teaches or arases vil damage te ilr alium chamber iner Warning Naver cs pa unit wan accel or amate caer Avertissement Na jamais retos apparsi salou avac das netoyants tana The incubator chamber shouldbe cleaned and disinfected poor to frst use Penod cleaning and dismfecton are required to prevent microbiological contamination Cleaning Pertorm the below steps to clean the incu 1 Remove of the interior parts shelves racks and any action assembled 2 Clean the incu deionized water w with a mid soap and solution including all comers Do not use 3 Rinse wth distiled water and wipe cry with soft cloth Take special care when cleaning around the door switch temperature sensor heads and chamber power outlets to prevent damage dry or alow the chamber to air dry before disinfecting or returning to use Procedure continued on next page E MAINTENANCE CONTINUED Disintecting Disinfect he incubator on a regular basis Perform the flowing steps to infact the workstation 1 Tum the unit ot Carryout your laboratory cinical or production space disinfection protocol 2 possible remove al interior accessories shelving and other non aitached items trom the chamber when disinfecting corner ne incubation chamber Take special care when disinfecting around the temperature probes 3 Disinfect t
3. The incubator shouid already be running in Day Mode with he auto cycle tuned of during tne calibration the auto cycle itt is running by pressing and holding the Start Cycle button for 2 seconds b I not already in the Daytime Mode place the incubatorin Day Mode wih the Day Time Temperature indicator LED tuminateq Day Mode CORT on est page OPERATION CONTINUED 3 Allow the temperature to re stabilze the door or access port have been opened aer the 8 hour tahikzaion the unit was place in Nighttime mode during or fter he stabilizator period sterenoo device temperature measurement should not change tor at least 1 hour in order or Ine chamber lobe re stabilzed 4 Once the temperature has stabilized compare the reference device and Reterence Device th incubator display temperature reading f the readings are the same s he differance between the two falls within the acceptable range of your 205 SSborstry protocol the incubator is calibrated for Day Mode temperature Advance to the Night Calibration steps on page 29 HA lta one readings and hat otse ia Reference Device ase your labor procol acceptatie rage place fhe cubre abraten mode See the nex stop 2117 Batore pacing the unit in calbration mode allow the temperature 1o re stabiize the door has been opened This wilrequre a wat cf at least one 1 hou
4. the chamber o stabilze with no uctuations of 0 1 C or greater Failure to allow at least 8 hours win result in an inaccurate temperature calibrator The Day and Night temperature set points require separate calibrations even if both are set to the same temperature This is due lo heat generated by the day luminaton LED ight bars when the Day made active This procedure requires a temperature reference sensor device Aways use a reference device aitrated to at last 0 1 C For best results use digital device witha wira sensor probe o take ings without sturing the chamber temperature Do not use ar Part 1 Calibrate the Daytime Temperature leah thermometer 1 It you have not already done so place the temperature sensor of tha reference device insid tha incubator as close as possible to na chamber geometric center Check that Ihe sensor s not n direct contact wih the shelving Note A thermocouple sensor probe s sleeve may be taped to the shelving as long exposed copper end is 2 inches Sem above the shel An exposed sensor robe in eect contact with the shelving may experience heat sinking which can Test in an inaccurate temperature reading Note Sensor probes may be introduced into he chamber through the door access Place the stopper back into the Port over he wee aller introducing a probe The Stopper must be n place to perorm an accurate calibraton
5. The intensity of PID conroled heating or chiling i proportonal to tne diference between the measured chamber temperature and the Current temperature set point The freuency f heating cing pulses is derived from the rate of change inthe ference Integrator feedback sous th rate of heating or chiling the chamber temperature approaches the set point which helps prevent overshoot During normal operations with the chamber door closed the Petter device pulses heat or cold to he chamber almost continuously as indicated he green TEC Activated pio ight These aro short low poner pulses to compensate or deviations of hundredth degree Celsus 40 01 C As a sold state device the Poor also olfers significant maintenance savings cost The device can be replaced by service technician using a screwdriver and a nut driver It does not require a retigeration specialist with refrigeration loi replacement coolant fi and des not contain a system of fragile pressurized uid vessels Door Alarm The SRIPF incubator is equipped with a magnetic induction door alarm which activates when t decr is opened and ts two sensor components are moved out ot range of one another er 60 seconds When the alarm is active an audio alert wil sound and ne control pane display wil ae Leaving tha door open for extended periods disrupts the temperature uniformity and stability ol the incubation chamber and may result in sigicanty increased
6. 00 6 PM 18 00 9 PM 21 00 12 AM 00 00 Pressing Enter without adjusting the display wal save the unadjusted Nght Start Time evi the Time Sat up menu 3 Save the adjusted cycle Night Start Time Adjusted Time Saved a Press Enter after adjusting he Night Start Tim Display 10 The display wil ext the Time Set Up menu and resume showing the Curent ime Tho Timo Dispiy wis cease lining Temperature Display wil tum on Nighttime LED wil extinguish The Curent Sme LED wil iluminate D zi OPERATION CONTINUE Note The OTL should ship hom the factory sat o the maximum positon DISABLE THE OTL Set the OTL dial to its maximum positon This prevents the OTL system from intertering vith raising the temperature set point or the conducting the temperature display casbraton procedure 1 Tum the Over Temperature Limit control dial clockwise to the maximum postion indicated by the largest dot 2 Leave the Temper itl at ts maximum poston unti the Set Temperature and or Calibrate isplay procedures have been completed 3 See page 31 fornstuctons on how to set he OTL after the above procedures are completed OPERATION CONTINUED SET THE DAYTIME TEMPERATURE SET POINT Perform the following steps to acust the temperature sat point ot he Daytime Mode t that ot your laboratory or study protocol The incubator cames rom ne factory with a Day set poi
7. Display Sel Lp 20 Set Curent and Cycle San Times 2 Disable 23 Set the Daytime Terporalure Paini 2 Set the Nighttime Temperature Set 25 Caibrate Temperature Display 26 Set Over Temperature Lim Tum Of On 32 Launching the Auto Cycle Ending the Auto Cycle E Loading the Incubator E Attaching equipment lo ihe interior Accessory Que as Humana the Incubator E Managing Condensation EJ USER MAINTENANCE a Cleaning and Disintecting E Maintaining Atmospheric lptegriy Components UNIT SPECIFICATIONS s Weight se Dimensions 39 Capacity E Shel Capaciy Weigh 40 Temperature 40 Power 40 PARTS LIST i INTRODUCTION Thank you for purchasing Sheldon Refrigerated Peis Fly Incubator We know that in today s competitive marketplace customers have many choices when comes to constant temperature equipment We appreciate you choosing ours Cur continued reputation as leading laboratory product manufacturer rests wi your satisfaction Sheldon Manufacturing nc stands behind our Products and we wil be here if you need us These incubators are intended or professional industrial or educational use for frui y They aro not designed for use in hazardous or household locations Before using the incubator read this entre manual careful to understand how to install operate and maintain the unt ina safe manner Keep
8. chamber The power switch onthe front panel controls power to the accessory aul This outlet can power equipment such as magnetic stirers rockers et Do not attach any equipment drawing more han 1 to this outlet Accessory equipment may produce addtional heat This heat could affect the temperature range and uniformity o this incubator Verl that the incubator operates within your protacls required temperature range when the accessory equipment is installed and operating HUMIDIFYING THE INCUBATOR Placing small numberof open or breathable media containers in the incubator chamber may tead to excessive drying ot sample medi Unusually dry environmental conditions may also contribute to sample drying To counteract this Sheldon Manufacturing offrs an optional humidity colection pan and tubing accessory kit Part Number 9900708 The kit redeects moisture that normally condenses onthe heat sink fins ot he Peier TEC H heating and cooing device and uses itto humidity the incubator After ordering and receiving the K place the stainless steel on the lowest chamber surface Connect he tubing that comes with the kit to the port on the back of the Petr duct cover Run the tubing down back of the incubator behind the shelves and secure the end of the tubing inside he pan The pan is supplied wih a copper slug to heip prevent microbial contamination The humidification kit is intended tor use while running smal ads L
9. elecinety and cause serious injury Aways use the power cord supplied with the una or an identical replacement cord Avoid damaging the power cord Do not bend it excessively step on or place heavy on A damaged cord can be a shock or re hazard Never use a power cord i itis damaged Position the unit so that the user can quickly unplug the cord in the event of an emergency Use only approved accessories al INTRODUCTION CONTINUED ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves al ofits products As a result engir and improvements are made fram ime to ime Therefore some changes modifications improvements may not be covered in this manual f your unis operating characteristics or appearance diers rom those described in this manual please contact your Shel Lab dealer or for assistance ering changes a CONTACTING ASSISTANCE you are unable to resolve a technical issue with your incubator please contact Sheldon Technical Support Phone hours for Sheldon Technical Support are 6am 4 30pm Pacific Coast Time west of the United States UTC 8 Please have the flowin information ready when cating or Techrical Support he model number and the serial number see page 9 EWALL toch shallab com PHONE 1 800 322 4897 extension 4 or 503 640 3000 FAX 503 640 1968 Sheldon Manufacturing INC P O Box 627 Comalus OR 97113 RE
10. power use as tha controller attempts o compensate forthe disruption The alarm may be turned off using the Arrow buttons Please see Turn Alarm procedure on page 32 Accessory Compatibility Make sure that any accessory equipment you wil be using inside an SRIPF incubator can safely operat within your selected temperature range Powered accessories such as sirrers or shakers hat produce significant waste heat may interfere with the temperature uniformity and operating range ofthe chamber Additionally fuit ies can produce aerosolized acidic metabolic byproducts that may affect the long term performance of accessory equipment The Over Temperature Limit System OTL system is a mechanical heating cut oif system provided with ts own temperature sensor probe inthe incubation camber When set he OTL system prevents runaway heating in the event of a ature of tha microprocessor controler or ts thermometer probe wl the TEC H is heating tne chamber The OTL does this by depowering the TEC H heating cuits whenever the temperature n the incubation chamber exceeds the OTL seting Typically the OTL is set 1 C above the digital controller temperature set point Because of its as a mechanical system and its lack PID anal tne OTL cannot deliver the same degree af temperature stability and measurement precision as the dal controler The OTL System should oniy be used as a means of heating regulation for the incubati
11. show the blinking adjustable Night Temp set point 3 Use the UP and DOWN arrow keys Io adjust Ps binking sel poit Adjusted Night Set Po I nether key pressed within 5 seconds the wil exit the set point adjustment and resume showing the current chamber temperatura Tre last displayed set point wi now be saved asthe new Nightime mode temperature set point Wart dened emperar sa pot rosse The Convo Shane Coding a Temperature dispiay will cease flashing ka cried sd b The Time display wil luminate The Temperature depisy wis revert to showing the Current chamber az temperature The adjusted set point is now saved The incubator wa beg cooing or heating Io actieve the new se pont Ea oT OEE LAB OPERATION CONTINUED CALIBRATE THE TEMPERATURE DISPLAY Temperature calibrations are performed to ensure that the tar air temperature inside he incubation chamber ure splay matches the actual soften as required by your laboratory Protocol ar regulatory compliance A temperature shouid be performed afier changing he operating temperature set paint or instaling the incubator ir conditions may affect urit performance Allow the incubator to run at its Daytime Mode set point workspace as ambient smperature undisturbed with no door openings for 8 hours prior to this procedure This
12. shows the Daytime start time and the Nighttime start time separately Three LED lights immediately below the Time screen indicate the present mode of display Current Time adjustable Daytime stan me and the adjustable Nighttime start ime Temperature Display Marked SET TEMPTERATURE this digtal display shows the air temperature in the incubation chamber accurate to 0 1 C The display also show the adjustable temperature set points of the a Day and Nght modes as well as display value adjustments during temperature calibration Jt below the Temperature Display are two LED lights Day Temp Night Temp These indicate which mode the incubator is running in Day or Nighi as well as which mode s temperature set point being displayad when adjusting the set points LAB CONTROL PANEL CONTINUED Enter Button The ENTER button is used in the Time Set Up menu to save adjustments to the Currant Time as as the auto cycle Day and Night start times The Enter buton can also be used to serol forward through the menu Mode Button Pressing and immediately releasing the button enters the Tima Setup menu Pressing and holing the Mode button for 5 seconds switches ne incubator between the Daytime and Nightime modes when the day night auto cycle is not running Cycle Start Button Pressing and hong this button tor 2 seconds will launch the day night auto cycle or termins cycle itis running T
13. AB PERATION CONTINUED MANAGING CONDENSATION Excessive condensation in the incubation chamber may crate leaks around the cham may cause corrosion damage if alowed to continue for significant lengths of ime door and Condensation appears wherever the humidity level in the incubator chamber reaches the dew Point The dew points the level ot humidity at which the air cannot old more water vapor T Warmer the air the more water vapor hold Evaporating sample media can be a source of chamber humidity As the level ol humidity rises in an incubation chamber condensation wil frst appear on surfaces hat are cooler than the air temperature Near the dew point condensation wil form on any tem or exposed surace that is even slighty cooler han the air When the dew point is reached condensation forms on nearly all exposed surfaces Managing condensation pimarly depends on either lowering the hum level or increasing the air temperature in the incubator chamber Note ising or taling ar pressure om weather wil adjust ha dew point up and dawn in smal increments he relativa humidity in ina incubation chambers already near the dew point barometric uctuations may push across the dew point thrashold Note Thin air at higher atitudes hols less humidity than the denser air found at or near sea level T condensation is torring in the incubation chamber check the following fs the chamber door closing and latching properly Is t
14. CEIVING YOUR INCUBAT Batore leaving the factory al incubators are packaged in high quality shipping materials to provide Protection transportation related damage When unit leaves the factory sate deivary becomes the responsibilty ofthe carrier Damage sustained during transit not covered bythe waranty This makes t important that you inspect your incubator tor concealed aes or damage to is interior and exterior when receiving i W you nd any damage ta the uni fo ow tne carers procedure for aiming damage or loss The orientation photos found on the folowing pages may serves as a useful visual guide lor INSPECTING THE SHIPMENT inspect the shipping carton for damage Report any damage to he carrier service that dlvered the incubator he carton is not damaged open he carton and remove the contents The unit shouid come wih an Instaliation and Operation Manual warranty card and a Cerificate of Compliance Verity that the correct number of the following accessories are present shoves Sheli Mounts Shel Mounts ELE E Standard Bracke ETTI Leveling Feet ie Side Alr Duct Panels A 115V 2 5 meter NEMA 5 15P power supply cord The stapper should come installed in the access inside the unit incubation chamber The incubator requires a temperature reference thermometer accurate to at least 0 1 C for performing temperature deplay calibratons The reference senso
15. FLY INCUBATORS 100 120 Voltage LAB Installation and Operation Manual SRIGPF SRI20PF Previously Designated erated Incubator Peltier FLY 100 120 Voltage Installation and Operation Manual Part Number Manual 4861674 1 Revision February 6 2015 Pictured on Cover SRIGPF Left SRIQOPF Right Those units are T V CUE listed as incubators and radiant warmers for professional industrial or educational use where the preparation or testing of materials is done at approximately atmospheric Pressure and no flammable volatile combustne materials are being heated units have been tested to the folowing requirements C2 2 No 61010 1 2012 C22 2 No 610102010 R 2009 ULGIDIDA 20102002 UL 61010 12012 EN61010 12010 EN61010 2 0102003 2 BLE OF CONTENTS INTRODUCTION n General Safety Considerations Engineering Improvements 5 Contacting Assistance 5 RECENING YOUR INCUBATOR s Inspecting the Shipment Orientation Photos H Recording Data Pls inarmaion H INSTALLATION 10 Location 10 Power Source 10 Liting and Harding Leveing Access Por Sloper Initial leaning and Deionized Water 2 Install Side Air Ducts 12 Shelving Installation 5 GRAPHIC SYMBOLS 2 2 15 CONTROL PANEL OVERVIEW 16 OPERATION Theory af Operation 18 Prepare the Incubator 20 Control ana
16. al oie your 208 11 the diference between the Day Mode temperature incubator readings and the reference deve stil fal outside your laboratory protocol afier trae calibration attempts contact Technical Support for assistance Three calibration attempts may be required to successully units hat are more than 22 C out of casio Contrusd on TBE OPERATION CONTINUE Part 2 Calibrate the Nighttime Temperature IIO 1 Place the incubator into the Night Mode b The Day Temp indicator xinguish The blue Night Temp indicator wil iluminate Press and hold the Mode button for ve seconds 2 Walt 1 hour to allow the temperature to stab ize after the Temperature display achieves the Nighttime set point The ference device temperature measurement should not change tor at least one 1 hour in order tor the chamber t considered stabilized wr Reterence Device 3 Once the temperature has tbe compare te reference device and citm depa temperat exige Vine rexinqs are Pe 196 B e croce bermeen teo lale win Pe acca your laboratory protocol the incuba tor Night Mode temperature The Temperature Calibration procedure 4 tees aditance beeen te vo readngs and hat let al Device Uzi warnen EE ah e pam esr no pestes a nes ser Belore placing the unit in castration m
17. arat Figure 5 Siding Shelf Mounting Figure 6 Sliding Shelf Mount Bracket Installation Bracket instalation 1 Insert the sling brackets two pairs of tabs front and back into the siots onthe shet standards See Figure 3 2 Side the bracket down so that the tabs are securely seated in the mounting slots See Figure 6 3 Secure the bottom fange of the sling bracket to the shelf standard rails by threading and crewing in 2 screws on the ront and back of the flange See Figures 7 and 8 Repeat the process on the opposite side of the chamber with the second siding mounting bracket 5 Hang 1 shelf tom the 2 installed siding mounting brackets u p Figure 7 Sliding Mounting Bracket Figure 8 Siding Mounting Bracket Screws Front Screw Installed ips LAB APHIC SYMBOLS symbols on interior and exterior surt de d passement de temperate repete dea positon CONTROL PANEL OVERVIEW Figure 9 SRISPF and SRI20PF Control Panel Power Switch The graen power switch on the panel controls all power to tha unit and ts systems The switch luminates when in the On postion 1 Time Display The green LED display labeled TIME on the left side of the panel operates a 24 hour clock 00 00 23 59 During normal operations it splays the currant timo When setting the start imes of the automatic day night luminaton and temperature cycle t
18. cTs SRI20PF Two 2 side air duct panels are included in the SRI20PF accessories Place the hooks on the top and bottom comers of panel inta the siots provided onthe shel standard mounting rails The SRISPF incubator does nol require side duct Figure 1 SRI20PF Air Duct Panel Note The duci panels play an important ole maintaining even heat disibution inside the ong incubation chamber of fna SRIZDPF Failure To install both duc panels may adverse Impact the temperature uniformity ION CONTINUED SHELVING INSTALLATION SRIGPF and SRIZOPF Standard Shelving Installation Perform the folowing steps to instal the SRISPF and SRIQOPF incubator standard shelves Figure 2 Standard Shelf Mounting Bracket Installation 1 _ Insert the 2 pairs of tabs on the bracket rant and back into the siots onthe shelf standard Tuis ofthe incubator chamber 2 Side the bracket down so that the tabs are securely seated in the mounting sts 3 Repeat the process on the opposite side ot he chamber wi the second mounting bracket 4 Hang one shet of the 2 installed mounting brackets Figure 3 Standard Mounting Bracket Figure 4 Standard Shelf Hung From installed Mounting Bracke mies LAB INSTALLATION CONTINUED SHISPF and SRI20PF Sliding Shelf instalation Note The SRISPF does not come wih a siding shall Siding shelves forthe SRIGPF must be purchased sep
19. ected 3 Release the up arrow a wil ash approximaty six imes Current Temperature b The display wil then revert to showing the curent chamber temperature The door alarm is now set to on and the temperature set point mot been changed End of procedure E OPERATION CONTINUE Note ir tre evant of a power outage te unit wil estar ha last made was operating in The incubator curent ime wil automaticaly adjust to compensate I active at the time of the outage the auto wil continue running and automatcaly advance to the Day or Night mode synched wih the updated current time LAUNCHING THE AUTO CYCLE start the day night Auto Cycle carry out the following step o 1 Press and hod the Cycle Start button for iwo seconds 3 The cycla wil now run indefinitely switching between Day Night modes unt E 2 The Program Active LED indicator next to the Cycle Start button wil luminate manual terminated ENDING THE AUTO CYCLE To terminate the Auto Cycle pertorm the following step oom EE e opas chr a Bs nc it E iy ER OPERATION CONTINUED LOADING THE INCUBATOR Place tems on the shelves inside the incubator chamber as evenly spaced as possible Good spacing allows for maximum air ulation and high degree of temperature uniformy ATTACHING EQUIPMENT TO THE INTERIOR ACCESSORY OUTLET Theincubator has maximum accessory outlet located inside the
20. he door gasket leaking Check the gasket Tor damage wear or sign of rienees or dryness Replace gasket f needed Are frequent or lengthy chamber door openings causing signifiant temperature disruptions and sriling the chamber surfaces t so reduce ne number of openinge ls the incubator exposed to an extemal fow of cold air such as air conditioning vent or a doar to cooler hallway or adiacent Block or divert the air or move te incubator Does the ambient humidity in the room exceed the stated operating range of 80 relative environmental humidity If so lawar the room s Empty the humidiy collection pan frequently Make sure tems on the shelves are evenly spaced to for good alow Wipe down and dry condensation whenever present in the chamber EI USER MAINTENANC A Warning Pero aribrarco or sees on is ur deromor poer ar rom power ugly Avertissement Avant fefisusr toute msinenanca ou entreten de apa bance le cardo secteur dei Ya hazardous material or substance has spied in the incubator immediately niata your she Hazardous Material Spil Containment procol Contact your local Site Safety Ofer and folow instructions per the site policy and procedures CLEANING AND DISINFECTING Note Do nal uae spray danera or that mipi ak rough aparing and wads and coat occa or Pt contain sans harm coatings Do rol
21. he green PROGRAM ACTIVE indicator to the right ft button when the is running Heating and Cooling Light The green pilot ight located beneath the label TEC ACTIVATED sluminates whenever the Pir TECH device is citing or heating the chamber The igh wal be on almost coninualy during normal operations as the unit makes continual small temperature compensations Set Over Temperature This control s equipped wih graduated dial The OTL is a mechanical heating cut of system that operates independently of he digtal temperature controller It guards against of the digital contol that wouid alow the chamber temperature to rise past the controller set point Please see Over Temperature Limit System description in the Theory of Operations section page 19 tor a more compllo explanation OTL Light The red piot ight marked OVER TEMPERATURE ACTIVATED iluminates when the Over Temperature Lima system cuts power to the Peltier TEC heating crus Under normal operating conditions this nestor should never tum on Fuse Located on the top back of the una inside the cord inlet the fuse protects against over curent conditons H the fuse blows the unit wit shut down The cause of a Blown fuse should be determined poor to replacing it LAB OPERATION THEORY OF OPERATION The SRIPF tamay of fy incubators is designed to provide a variable temperature and illumination environment suitable forth cutvaton and st
22. ho incubator using commercially available disinfectants that are non corrosive moncabrasive and suitable for use on painted steel and mil nish aluminum surfaces Contact your local Se Safety Offer fr detased information on tha disinfectants Sompstble wih your cultivation euturing applications 4 Do not use overly volatile disinfecting agents Chiorines amply and quaternary ammonias wil evaporate into the chamber environment Overtime the concentration inthe Chamber atmosphere wil continue to ncrease potently leading to inhibited growth or metabolic symptoms in sample populations End of procedure MAINTAINING ATMOSPHERIC INTEGRITY Periodically inspect the door latch vim catch and gasket for signs of deterioration Failure to tain the integrity of the door system shortens the ife span of the incubator ELECTRICAL COMPONENTS Electrical components do not require maintenance If the incubator fails to operate as specified please contact your Shel Lab Dealer or Sheldon Technical Support tor assistance please see Page EJ INIT SPECIFICATIONS incubators are 100 120 voltage units Please refer to the incubator daa plate to varity its specications Technical data specified applies to units wih standard equipment at an ambient temperature of 25 C and a votage fuctuaton ot 10 The temperatures specified are determined in accordance to factory standard folowing DIN 12880 respecting the recommended wa
23. iding up in he port but snot required Wires for thermocouples and other sensor probes may be introduced into the chamber the access port The stopper may be put in lace over the wire Seal any gaps using a non adhesive tape that wil not leave adhesive residue when e removed The port stopper must be in place during operation tor the incubation chamber to achive its specified temperatura stabilty and uniformity levels INSTALLATION CONTINUE INITIAL CLEANING AND DEIONIZED WATER The incubator interior was cleaned at the factory but not steriized B may have been exposed to contaminants en route during shipping Cleaning and dsifecing the chamber and shelving as wal as the ar duct panels ofthe SRI2OPF prior io placing the unt in operation lawers the chance f microbiological contamination See the Cleaning topic in the User Maintenance section page 37 tor mare formation on how o clean and disinfect the incubation chamber Remove al wrappings and coverings rom shelving prior to cleaning and installation Never use deionized water tor cleaning the incubator Whi DI water s useful in variety of laboratory applications itis an aggressive solvent that attacks most metals Use of DI water voids e manufacturing defects warranty Use ol distiled water the resistance range of 50K Ohmiem e IM Ohmicm ora conducti range of 20 0 uSlem fo 1 0 uS cm is recommended for cleaning and any humidtying appications INSTALL SIDE AIR Du
24. ll clearances of 10 ol the height with and depth of fne inner chamber AI indications are average values typical for uns produced in the series We reserve the right to alter technical specifications at al times Model Shipping IE Dime In inches Model Exterior W x D H Intetlor W x D x H In Centimeters Exterior WxDxH Interior W CAPACI Model Cubic Feet Liter IT SPECIFICATIONS CONTINUED Shelf E Em TEN TE a ri Model voltage gt Frequency G LAB PARTS LIST Description Part Access Port Stopper 7750517 Gest Magne Door 25 inches X 28 inches aaa Funidiy Reservar Pan and Tung Options Accesso Li Sing Sha ouning Ki 2 Backs Em t you have the Part Number for an item you may order the item directiy trom Sheldon Manufacturing by cating 503 646 3000 Ext 3 If you are uncertain that you have the correct Part Number or you need hat specific part please contact Sheldon Technical Suppor for help at 1 800 322 4897 or 503 640 3000 extension 4 Please have the model number and serial number of the unit ready as Tech Support d this information to match your unit with its correct Sheldon Manufacturing Inc PO Box 627 Cornelius Oregon 97113 EMAIL tech Sheliab c
25. loy an alternative power saving thermoelectric cooing and heating device The TECH operates using the Peter which the junctures between two elected conductive pates generate gradent of temperature diferences In cher words curent between the conductors produces low o heat away irom one plate to the other The recon of this gradient can be tipped by reversing the drecton of the curent The sandwiched TEC conductors effectively operate as a reversible heat pump that is signiicanty more efficient han a compressor element pairing This power use efficiency resus in far less waste heat and a corresponding reduction in cooing demand placed on laboratory air condiioning systems Heating cooling of he incubation chamber is controlled by the incubator digital microprocessor controler The controller stores the Daytime mode and Nightime mode temperature set points The controller monitors the air temperature of the incubation chamber using a solid state sensor probe located in the chamber airstream When an air temperature deviation from the active set point is detected the controller pulses power to tha TEC H device to add or remove heat from the chamber Heated or chiled air is circulated through the chamber using a fan attached the TEC OPERATION CONTINUED The controler employs proporional integral derivatve PIO analytical teedback loop functions when measuring and controling the chamber temperature
26. not done so verity that the workspace power supply and incubator data plate requirements match Please see page 10 in Installation section the power cord into the earth grounded electrical outlet Place the Power switch in the ON 1 posto CONTROL AND DISPLAY SET UP Al initial power up the incubator wil run in its Day mode Do not launch the auto cycle or manually switch to the Night mode until a temperature calibration has been performed The incubator comes from the factory set to run at 20 C in bath tha Daytime and Nighttime modes Perf the folowing procedures to adjust the incubator ime and temperature parameters to those of your laboratory protocol well as alate he incubator n 1he new workspace environment 1 Set the Current and Cycle Start Times page 21 2 Disable the OTL page 23 3 Set the Day Mode Temperature Set Point page 24 Set the Night Mode Temperature Set Point page 25 S Calibrate the Temperature Display page 28 8 Set the Over Temperature Limit Control page 31 21 OPERATION CONTINUED SET CURRENT AND CYCLE START TIMES Enter the Time Set Up menu to set the display clock to your current local time Adjust the auto cycle Daytime and Rightime sta times it needed The incubator comes from the factory set to US Pacific Time The auto cycle day start is factory set to 08 00 Bam andthe night start is 20 00 1 Press and release the Mode button to enter he Time Set Up Fact
27. nt of 20 C Skip this procedure if your protocol requires the incubator lo be run at 20 C under day conditions wh the chamber ilumination on continualy Matching the set point to your protocol prepares unit or temperature calibration I auto cycle is active and you will be performing a temperature calibration after adjusting the set points terminate the cycle The unit must be run in Daytime mode to adjust he Day T Verily that he incubator is running in Daytime mode Advance to step 2i Program Active Light ON or fhe auto cycle is running m Nighttime mode terminate the and manualy piace tne un in Daytime mode Press o Sha hod no Cycle Start button for wo second The Program Ace Light wil extngush b To switch rom Nghtime to Day press and hoki the Mode tuto far 5 second Ta bue Nght Temm ma calor wl Sings ha Day Temp wil mia Place the Temperature display into tha Daytime Set Pant mode Pressand release the Up or Down arrow bution b The Time display wil go dark The Temperature display wil briefly fash the letters SP 3 The Temperature display wil show the blinking Day Temp set point d Aker the desired temperature set pointe selected release The corr Chamber Heating to The Temperature display wil cease flashing Achieve he New Set Point b Tho display wil revert to showing the current chamber air temperature adjusted set point is now saved
28. ode allow temperature Io re stabilize the door has been opened This wil require a wait ot at least one 1 hour Faire 10 do so wil Wtr result in an inaccurate calbraton SE TO GE 1 OPERATION CONTINUED Nighttime Temperatur 5 Place the unit in ts temperature calibration mode and catbrate Press and hold both the UP and DOWN arrow butions Simultaneousy b The Temperature Display wil show the letters CO then begin flashing the current display temperature value Use the Up or Down arrows to adjust the display temperature Value unti matches the reference device temperature eating Tie wil corect he splay error Corrected Display d an arrow key is not pressed for five seconds the Temperatura Display wil cease ashing and store the last displayed value the curent chamber temperature DENT CCCII EE Ene 7 Allow the incubator sit tor 1 hour undisturbed to stabiize after it has achieved the set point wih the corrected display Failure io wait until the urit is fly stabized wil esu in an inaccurate reading 8 Compareierelerence devica reading wih the incubator temperature display the reference device and the incubator temperature display readings are the same or he ference fal within the range S your laboratory protocol the incubator is now calibrated for temperature 3 the two readings are not the protocol rapeat
29. om INTERNET http www Shellab com PHONE 1 800 322 4897 503 640 3000 FAX 503 640 1366
30. on chamber unt a aed controller or its thermometer probe be repaired or replaced The OTL wil not prevent a rise in heat caused by a complet failure of the Ploor TEC H itself With he loss of the chiling function the chamber temperature wil rise othe ambient room temperature plus or 2 e E OPERATION CONTINUED Note Seting up the Incubator for use in new workspace environment requires an B perod for the unt fo come up fo temperature and sit undisturbed This done lo achieve Thermal stabilty Thermal stabilization at the operational set point necessary to achieve an accurate on site temperature calibration See he Calibrating the Temperature Display procedure on page 28 for more information PREPARE THE INCUBATOR Perform the folowing steps to prepare the incubator for use each me it is installed in a new 1 you have not done clean and disinfect he incubation chamber if required by your laboratory protocol Pease seo page 12 in the Installation section 2 You may place a temperature reference device or ts sensor probe inside the incubation chamber at his time Placing a probe in the chamber now tor taking remote readings saves Signieant teme during fne Temperature Caibraton procedure See page 26 for probe placement instructions 3 Vert that the access port stopper is in place inside the incubation chamber The stopper can bo carefully sealed aver the wire o a temperature probe M you have
31. orage o ut fies Drosophila melanogaster The incubator runs in one of wo modes Daytime and Nightime In is Daytime mode the incubator provides continual ilumination in the incubation chamber using two side mounted white ight LEO bars n Night mode the chamber is left dark though a small LED bar at the top ofthe chamber wit luminste whenever the chamber doors opened The Day and Night modes have independent programmable temperature set points Both are set 1 20 C at he factory but can be adjusted to different temperatures in order provide thermal variation between the modes Tho SRIPF incubator comes rom the factory set to run indefinitely in the Daytime mode Users can manually switch between and the nightime mode by pressing and holding the MODE button The incubator is also provided wih a Day Night Auto Cycle which when launched wil cycle the tietwoen the modes untsi manually terminated As set at the factory the cycle transitions between modes are 12 hours apart These start time points can be adjusted using the incubator contol During Day mode operations the incubator diit controler automaticaly compensates for a small amount ot extra heat generated bythe active LED ight bars i the incubation chamber Heating and Cooling Conventional low temperature capable incubators rely on a competion between a refrigeration compressor and a resistive heating element to achieve sable chamber temperatures SRIPF incubators emp
32. ory Curent Time The Current LED wil stay st The Time display wil show a blinking adjustable ime eating Z the Curent ime to match your local ime using the UP and DOWN amus Adjusted Time 3 24 hour clock ftempon evening times are 12 hours rester than the 12 hour clock PM equivalents Example 1 PM 15 00 6 PM 18 00 9 PM 2100 12 AM 0000 P sng Enter without adjusting the display wil skip to setting the Daytime start 3 Save the adjusted Current tim Press and release the Enter button b This saves the flashing time shown in the display as the new Curent time Adjusted Time Saved depay wil asomarkaly advance tothe auio Daytime Start Day Start Time Display wit continue to ash but now displays the adjustable Dayime start ime The Current Time indicator LED wil extinguish 4 The Daytime LED indicator tuminats PSR CORT est page OPERATION CONTINUED Set Times Continues Use the UP and DOWN arrow keys to adjust he cycle Daytime Star Time Adjusted Day Start Day Start Time and skip seting ne Nighttime start ime Adjusted Time Saved 6 Save the adjusted cycle Day Start time Press the Enter button ater adjusting the Day Star Time Cycle Night Siart Factory Sening 7 Tho display wit automaticaly advance to the auto cycle Nightime Start 9 13
33. plore other incubator options suited to your laboratory or production LOCATION When selecting a location to instali the incubator consider all environmental conditions that can fective temperature range unsormiy and stabilty ot he unit For example Ovens avtoclaves and any device that produces significant radiant heat Heating and cooing ducts or other sources of fast moving air currents areas Diocisunlgnt Power SOURCE Note The electrical supply othe incubator must conform to national and local circa codes Aways postion the unit so thal the end user has access to the power cord in ih emergency When selecting a location for an SRIPF incubator very that he earth grounded wall power source matches the voltage and ampere requirements isted on th incubator data plate Supplied voltage must not vary more than 10 from the data plate rating Damage Io the incubator may result i supplied voltage varies more than 10 Damage lom a power supply that does not match the dala plate requirements is nol covered by the manufacturing defect waranty Use separato circuit to prevent loss of product d to overloading or circuit failure These incubators are intended tor a 50 60 Hz at the flowing amperages Er ET These units are provided with an 115V 2 5 meter NEMA 5 15 power supply cord 11 INSTALLATION CONTINUED LIFTING AND HANDLING An SRIPF Incubator
34. r lo do so wi result in an inaccurate calibraton 6 Place the uniti ts temperature on mode and casbrate Press and hold both the UP and DOWN arrow buttons Simultaneouey b The Temperature Display wil show the letters CO then begin lasting te current display temperature value reading This wil correct the display error 4 an arrow kay is not pressed for ve seconds the Temperature Display wil cease flashing and store the last displayed val as the new curent chamber temperature valve SETS COTTE oR GE OPERATION CONTINUE 7 Alor correcting for the ait five 5 seconds a Tho Temperature Display wil cease flashing and store the ws b The display wil revert to showing the Current temperature The incubator wil now begin heating or cooling in order to reach Day set point with the corrected display value 8 Allow the incubator sit or 1 hour undisturbed to stabilize after it has achieved te set point wih he corrected display Failure to wait unti te unit is tuy stabilized wil rest in an inaccurate WEN reading 3 Compare the reference device reading wih the incubator temperature display the reference device and the incubator temperature display readings are the same or he ference falis within the range your laboratory protocol the incubator is now calibrated Tor temperature Ere Wo reading nol he same or t
35. r must be purchased separately For bost resulta use a digit relerence device with a wire sensor probe to take remote readings Remate readings cifer at east 1 hour of ima savings during each calibration Do not use an alcohol thermometer Please see the Calibration procedure on page 28 ofthe Operation section for more formaton Caratuly check all packaging betore discarding Save the shipping everything works property unti you are sure si RECEIVING YOUR INCUBATOR CONTINUED ORIENTATION PHOTOS SRI20PF RECEIVING YOUR INCUBATOR CONTINUED SRIGPF di raras RECEIVING CONTINUED REC The data number Record th ration fr fure reference Tho data plate is located on ho back of the unit on the top right Data Piate Date Plate Information LAB INSTALLATION incubator is intended tor use indoors at room temperatures between 15 C and 30 C 59 F and 86 F at no greater than 80 Relative Humidity at 25 C 77 F Alow a minimum of 4 inches 10cm between the incubator and walls partitions and 2 inches Sem clearance above the top of the incubator for unobstructed slow Position the unt so the end user has access to the power plug Operating the unit outside of these conditions may adversely affect the temperature range and stability For conditions outside of hose listed above please contact your distributor or Sheldon Sales to ex
36. rf the flowing steps to tum the door alarm of or on Note that once set to off the alarm wil not activate until the user has tumed it back on This procedure is applicable to units manufactured ter January 15 2015 or older units witha new door alarm control chip Turn the Door Alarm Off OFF 1 Place the temperature display in the adjustable set point menu for either the Daytime or Nighttime mode SetPoint indicator Press and release ther the Up or the Down arrow key wile in either mode i The display wil hen show an adjustable flashing temperature set point 2 Press and hold the Up arrow unt the temperature display reads dO e 3 UO indicates that door alarm off is selected do will ash aporoximaty sic times Current Temperature isplay wil hen revert to showing the current chamber temperature The door open alarm is now set to off and the temperature set point has not been changed Procedure continued on next page ai OPERATION CONTINUE Turn the Door Alarm On 1 Place the temperature display in the adjustable set point menu for either the Dayime or Nghtime mode Press and release ether Up or the Down arrow kay while inether mode The display wil hen show an adjustable flashing temperature set point 2 Press and hold the Up arrow until the temperature display reads dr Door Alum On 3 af indicates that door alarm on is sel
37. should only be ad by is bottom surfaces using proper heavy iting machinery such as a forklift pallet jack Handles and knobs are inadequate or ting or stabaizaton The una shouldbe completely restrained rom toping during ifing Transporting the unit whe ted is not recommended and may ba hazardous Remove al moving parts such as shelves and trays and secure the door in the closed position prior to fing the unt Do not attempt to move he unit while in operation or before the urit has cooled LEVELING The SRIPF Incubator must be level and stable for safe operation Each incubator ships wh tour evang fee Insert one leveling foc into each of the four holes in the bottom comers of the workstation Adjust he foot at each comer unti the workstation stands level and sod without rocking To raise a toot tur itin a counterclockwise direction to lower a foot tum itin a clockwise recton To prevent damage to feet vise in anspor tum the fetta the maximum counterclockwise 2700506 ACCESS PORT STOPPER Each SRIPF incubator is provided with an access port located back wall ot the h incubation chamber The incubator shipped with one rubber access port stopper installed in the port inside the chamber The stopper should always be installed n chamber side to obtain the best temperatura undormity and prevent Condensation rom forming inside the port A second stopper may be installed on the outside of tp uni to prevent dust rom bu
38. steps 5 8 Ye or fall outside your laboratory 10 the Nigh Mode temperature readings of tie incubator and tha reorance device si tal outside your laboratory protocol aer three calration contact Sheldon Technical Support lor assistance These calibration attempts may be required successfully units natare more than 22 C out of calbyxalion Enda pore OPERATION CONTINUED SET THE OVER TEMPERATURE LIMIT The incubator temperature must be stable and running at ts highest set point for at least 1 hour pore seting the Over Temperature Limit Ifthe Day Made wil be running warmer than the Night Mode set the OTL when the Day is active and at its ul temperature Perf the following steps to set up the Over Temperature Limit system for normal use 1 I you have not done so already tur Set Over Temperature Limit contol dil t maximum positon lows he Set Temperature conii to stabilize 2 Tum the Over Temperature Limit contol dal counterclockwise untl the rod Over Temp Lim Aetvated ight fuminatee tum pe Over Temperature Lim con lock uni ha ror Temp Lint Activated ine of Slop tring ta control 4 Leave the OTL control sight above the activation point This sets the Over Temperature Limit set to approximately C above he curent temperature set point End of procedure OPERATION CONTINUED TURN ALARM OFF ON Pe
39. this manual available for use by all workstation operators Ensure that all operators are given appropriate taining prior to using the incubator Note This ont is designed for the growth culivalon incubaLor amd storage of ft Ties Draeaphia melanogaster Improper use of this unit wil void the manufacturing defect waranty Other units are specitealy manufactured for BOD applications Consult your She Lab dealer or customer service representative in order Io identify another model suitable for our application JENERAL SAFETY CONSIDERATIONS Note Falure 10 falow the guidelines and inavuctons im tis manual may create a protecion impairment by cisabing or interfering with the unit safety features This can result n injury or death Your incubator and ts recommended accessories are designed and tested to meet strict safely requirements Only use this equipment for ts intended appscation Any alteratons or mocifcatons vod he warranty For continued safe operation ot your incubator aways follow basie safety precautions including Folow al ical ordinances in your area regarding he use of this incubator If you have any questions about local requirements please contact the appropriate local agencies The power suppl for the urit must be eath grounded electrical outlet ihat conforms to and local elacrcal codes the Incubator is not grounded properly parts such as kobe and controls conduct

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