DM0412 Clinical-Centrifuge Manual
Contents
1. 2 Rotor chamber N CAUTION Oo Do not directly pour water neutral detergent or disinfectant solution into the rotor chamber otherwise fluids may leak into the drive units and cause corrosion or deterioration to the bearings O If the rotor needs cleaning clean with cloth or sponge moistened with a neutral detergent solution Sterilize the centrifuge by wiping with a cloth moistened with 70 ethanol solution 3 Drive shaft O We recommend regular maintenance for drive shaft You can wipe the drive shaft with soft cloth and then apply a thin coat of silicon grease 4 Door O Clean and sterilize the door using the same method as the section 1 above 5 Rotor O To prevent corrosion remove the rotor from rotor chamber If not in use for a long term then detach the rotor lid and turn upside down to dry the tube holes and keep clean O For sample leaks in the rotor rinse the rotor with water Apply a thin coat of silicon grease to the rotor when it is completely dry O The rotor should be checked every 3 months to ensure the tube and rotor holes keep are clean and apply a thin coat of silicon grease 10 Troubleshooting 10 1 Possible problems and solutions This centrifuge has a self diagnostic function If a problem occurs an error warning code will be displayed on the time display screen and the operator can determine the malfunction with the alarm code below Nothing appears on the screen when Building power circuit2. pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and 1f not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense 3 3 Required Operational Conditions 3 1 Basic operational conditions 1 Power 110V 240V 50Hz 60Hz 3A 2 Ambient temperature 2 C 40 C 3 Relative humidity lt 80 4 No vibration and airflow around 5 No electric dust explosive and corrosive gases around 3 2 Transport and storage conditions 1 Storage temperature 40 C 55 C 2 Relative humidity lt 93 4 Installation This section describes the instructions that you should abide when install the centrifuge to ensure your safety and the optimum performance Before moving the centrifuge the rotor must be removed AN WARNING O Improper power supply may damage centrifuge O Make sure the power source conforms to the required power supply before connecting 4 1 Location 1 Place this centrifuge on a firm flat and level surface ensure the four feet of this centrifuge stand on the counte
3. 13 JO MA MI o AA A A 13 NUR OIO oratoria 15 euke ROPA A TTTTTTTTTTTTETTTETIIS 16 3 Ordeno INDOIMA O a a a LL E OO LAKO VEK T ENEO 16 ZI ia MIN TTT TETTE A TET TTT 17 e iia A a a ma E LAS man ES ske e e 17 AZIANA FLANO E e TA e i la o o a iv blo 17 Aller sales ery C C A LAAT TT a a TTT TTT 17 Copyright No part of this manual may be reproduced or transmitted without prior written permission of Scilogex We can not be responsible to inform at real time if the outline and specifications of centrifuge are subject to change for improvement VERSION201204 Safety Reminder Common safety precautions Carefully read the following safety precautions for a thorough understanding O Follow the instructions and procedures described in this manual to operate this centrifuge safely O Carefully read all safety messages in this manual and the safety instructions on the centrifuge Safety messages are labeled as indicated below They are in combination with signal words of WARNING and CAUTION with the safety alert symbol Mto call your attention to items or operations that could be dangerous to you or other persons using this centrifuge The definitions of signal words are as follows A WARNING Personal Danger Warning notes indicate any condition or practice which 1f not strictly observed could result in personal Injury or possible death A CAUTION Possible damage to centrifuge Caution notes indicate any c
4. O to modify values Release the button after 5 seconds and the centrifuge will return to normal operation mode and run according to the new value If the set time value has been modified the operation time 1s not affected and will continue 3 Warning display If an error occurs during the operation the centrifuge will brake to stop automatically and display the error code on the time display area The error code can be checked in the table 10 1 and corrective actions can be applied accordingly 8 1 3 End the operation 1 The centrifuge will brake when it reaches the set time or C2 button 1s pressed When the rotor stops rotating the centrifuge will start beeping to alert the operation has finished 2 Open the door The door can be released automatically when the operation has stopped With the door closed you are able to press the button to open it After ending the operation the program will store the setting parameters of this operation and will recall these parameters when restarting the program 3 Open the door and take out the rotor and samples 9 8 2 RCF operation 1 Turn on the power switch 2 Seta RCF Relative Centrifugal Force value AN CAUTION e Do not exceed the allowable maximum RCF value of the rotor and adapters SELEC O Press the select button and choose speed unit xg the speed symbol will flash into RCF value input status O If no button is pressed after th
5. breaker Remove the trouble and turn the POWER is turned on trips on the POWER Abnormal vibration e Rotor do not match with Install again the rotor spindle e Weighting scales install e Samples are imbalance symmetrically E 02 e The door opened in running e Close the door immediately Door fault START l eClose the door and then start e The button is pressed to operate While the door opening E 06 e The setting speed exceed the Modify the speed value Set wrong speed allowable range E 10 86 e Read the service manual e Contact with service center Table 10 1 Possible problems and solutions uos e dstp OUI 94 uo paJeadde apo wey O Alarm codes E 1 E 9 are related to incorrect operation programming You can continue running the centrifuge after implementing corrective procedures 10 2 How to open the door 10 2 1 In the case of power on N CAUTION The door just can be opened while the power is on and rotor stops rotating 1 Turn on the power switch release the door automatically 2 The door will be released automatically once the operation is finished 3 Itis available to release the door by press 2 button once the rotor stops 12 10 2 2 In the case of power outage The door cannot be opened automatically if there is a power outage It is available to be opened manually as follows 1 Ensure if the rotor has stopped rotating O Listen carefully to ensure no rotati
6. rotor is attached with a lid ensure it is tightened before operation O If any abnormal condition occurs during operation please stop it immediately and contact our service center Notify the service center is a warning code if displayed O Vibrations are likely to damage the centrifuge contact our service center if abnormality observed 1 Specifications Maximum RCF 2490 X g increment 100xg Maximum capacity 10ml X 12 15ml X 8 Timer 30 seconds 99 minutes HOLD continuous operation Driving Motor Brushless DC motor 4500rpm 300 4500rpm increment 100rpm Safety devices Door interlock over speed detector over temperature detector automatic internal diagnosis Additional features Speed RCF switch Pulse operation Processing display Voice reminder 2 Declaration of Conformity Construction in accordance with the following safety standards EN 61010 1 EN 61010 2 10 Construction in accordance with the following EMC standards EN 61326 1 FCC Part 15 Subpart B IECS 001 Associated EU guidelines EMC guidelines 2004 108 EC Instrument guidelines 2006 95 EC This ISM device complies with Canadian ICES 001 Cet appareil ISM est conforme a la norme NMB 001 du Canada Changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment NOTE This equipment has been tested and found to comply with the limits for a Class A digital device
7. 9 DM0412 19200316 A12 10P Plastic rotor 4500rpm 15m1 8 used with DM0412 19200317 A10P15 Rotor adapter used with A12 10P 12pcs pk 19200318 A A10P15 Rotor adapter mat used with A10P15 12pcs pk 16 14 Warranty 14 1 Warranty of centrifuge This centrifuge 1s guaranteed for two years from the date of delivery provided that it has been operated and maintained properly 14 2 Warranty of the rotor The rotor 1s guaranteed for 5 years from the date of delivery upon manufacturer Please pay attention do not use the rotor once 1t has been corrosion or fatigue damage The warranties of the centrifuge and the rotor become invalid in the case of the following conditions even 1f within the guarantee period expires 1 2 3 4 5 6 7 Failures caused by incorrect installation Failures caused by rough or improper handling Failures caused by conveyance or relocation after installation Failures caused by unauthorized disassembly or modification Failures caused by using non standard spare parts or accessories and unauthorized modification of the rotor or centrifuge Failures caused by natural disasters including fire earthquakes and so on Consumables and parts have a limited guarantee period After sales service In order to ensure to operate centrifuge safely and efficiently it is necessary for regular maintenance If centrifuge has problems do not attempt to repair it by yourself Contact our sales
8. USER MANUAL DM 0412 Clinical Centrifuge SCILOGEX LLC www scilogex com Before using centrifuge please carefully read this user manual for efficient operation and safety Contents SATO ORIG seko p IN a o ERNE Ne eT eee l kS peci eatoni oe ee 3 2 Declaration Of Conformity usura dd 3 3 Regured Operational CONTAINS xu a o a A a o l ko ko Ko 4 3 E Basic ODEFANONAGONIIIIONS sa Sera rnr EA a EN ERO E dE ORo Eok a a ea 4 3 2 Transport and storase cond ioNS sua e o a 4 A rim AO AAA AS AAA A EA AT AAA A NS 4 A eaters eae ae st ee settle oleae A IMI 4 4 2 Connection of the power cord and grounding oooocccccnooonoooooooonon nono nono nono nono non nono nono nn nono nono nn n nn nn n nro nono 5 De UIC UU ae nace O TTT ITT TT 5 6 Operation Pad usa 6 Al ee Ie a E TIO wae ae Ie ee RIO 7 sl Prepare ihe Sample Soniai a a E E E O dedeoteas 7 T2 AMECA AMP ESO IDES a E Ka E NE E NE E EO OS T KEEP THE TUDES Dl di rr A A A UN EN 7 SAJ a jo sedas POLO L irane E E E EE EE T EN 7 7 5 Symmetrically load centrifuge tubes into rotot occcccccncnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnos 7 Sa OPETI ON ss a a a a a a aaa aa a aD aaa 8 Sl Normal OP AIN ida 8 SS A A A A 10 DSi USS Opel ACI ON par A E GO En KON E o E Sao 10 Ou Maintenance a ii 10 A I 10 OI FO DIESNOONAS dido 12 10 1 Possible problems and SOMA ONS acarrean isis 12 IA AN A a uk ou a e ia A ak bo 12 11 Instructions for the rotor and UDS AS dde
9. UTION Make sure the rotor lid 1s securely fixed on the rotor as well as the rotor and shaft are tightened Otherwise the rotor may be moved off while rotating and cause damage to the centrifuge and rotor Firmly tighten the rotor lid to the rotor 8 Operation CAUTION Do not push or lean against the centrifuge while 1t is running Do not run the centrifuge when fragments or sample solutions are left in the centrifuge chamber Always keep the centrifugal chamber clean If the centrifuge makes strange noise during operation stop 1t immediately and contact our service center Notify them of the warning code 1f displayed 8 1 Normal operation Turn on the power switch centrifuge will display the running interface last time after passing the self diagnostic checks see figure 8 1 below Figure 8 1 the last running interface e Speed 4500rpm Running time 12 minutes O The door lock is released 8 1 1 Set the operation programs AELEC Press the button to select required program The parameter can be modified when the program is flashing Rotate the program button clockwise O to increase parameter value Rotate the program button clockwise O to decrease parameter value Rotate the program button cy faster and the parameter value will increase faster The minimum speed increment is 100 rpm the minimum time increment is 1 second 1 Set the speed SELEC O Press the select button until the speed rpm is di
10. and UV irradiation only ordinary sterilization can be used 14 11 2 Tubes 11 2 1 Cleaning and sterilizing tubes O Applicable X TE a tele la KONAS CC Alkaline higher than pH9 Cleaning fluids Mie Mme le a laj Neutral pH o o o Warmwater up to 700 Jo o o Ultrasonic cleaning Neutral detergent HD JO O o 115 C O Tkglom 30minutes O o jo Autoclaving 121 C LOKg enP 20 minutes x o O o jo o SUIULIJO E 15 to 30 minutes Ultraviolet sterilization 200 300nm x lg x Gas sterilization Ethylene oxide o jx lo Formaldehyde o o PA Polyallomer PC Polycarbonate PP Polypropylene UONEZI LID S Table 11 2 Cleaning and sterilizing conditions for tubes 11 2 2 Cleaning PC tubes PC material is low in chemical resistance against alkaline solutions Avoid using neutral detergents with pH higher than 9 Note that pH of some neutral detergents are still higher than 9 even 1f diluted according to the manufacturer s instructions Use detergent with its pH between 7 and 9 11 2 3 Autoclaving PA PC and PP tubes PA begins softening at about 120 C PC and PP at about 130 C Autoclave PA tubes at 115 C 0 7kg cm for 30 minutes PC and PP tubes at 121 C 0 1kg cm for 20 minutes If a certain temperature is exceeded the tubes may be deformed When use a sterilizing chamber please operate as follows 1 Place tubes in vertical position mouths upward If tubes are placed sideways they ma
11. e speed value has flashed after 5 seconds the input mode will be shut down O Rotate program button O to input a RCF value RCF increment 1s10xg 3 Set operating conditions The other operation please refer to the section 8 1 8 3 Pulse operation This function 1s used to remove the residual samples adhered to the interior of the tubes or for quick spins Note The button works only while the rotor stopped and the door is locked 1 Turn on the power switch and load the rotor to the shaft tighten the rotor lid and make sure it is in secured position and then close the door 2 The centrifuge goes into preparation mode and displays last running values 3 Press knob and hold the centrifuge will speed up to the setting speed While releasing the CASE knob during acceleration the centrifuge will start to decelerate and stop 9 Maintenance 9 1 Cleaning AN CAUTION O If do not follow the recommended instructions for cleaning or disinfecting this may damage the centrifuge 10 1 Centrifuge Ifthe centrifuge is exposed to ultraviolet rays for a long time the color of the door may be changed or the label may be peel off After using cover the centrifuge with a piece of cloth to protect 1t from direct exposure If the centrifuge needs cleaning clean it with a cloth or sponge moistened with a neutral detergent solution Oo Sterilize the centrifuge by wiping with a cloth moistened with 70 ethanol solution
12. ng sound can be heard 2 Insert a screw driver into a hole to open door Oo Holes are located on the left and right sides of the unit O Inserta screw driver into the two holes and push forward to release the door 11 Instructions for the rotor and tubes AN CAUTION O Read the instructions thoroughly to properly load and use rotor O Do not exceed the allowable maximum speed of rotor tube and adapters etc Ensure the allowable maximum speed of adapters is lower than the rotor s maximum speed 11 1 Rotor instructions 11 1 1 Rotor structure adapter screw nut rubber cap Rotor body mat Spindle hole Figure 11 1 The rotor structure 13 11 1 2 Available rotors and adapters Dimension Rotor Maximum Tubes DxL Adapters type speed rpm mm A10P15 1 5 5ml vacu 12 13x82 4500 2218 mat A10P15 13x106 11 2490 4 7 ml vacu 12 4500 16x75 9 8 2218 A12 10P 8 5 10 ml vacu 12 16x107 A10P15 a A10P15 EU ml 12 11x66 mat collection tube 12 7 5 8 2 EU ml 15x92 A10P15 4500 11 2490 collection tube Table 11 1 Rotors and adapters 11 1 3 Notice The centrifuge rotor can separate samples with a density lower than 2 0g ml If the samples density is over 2 0g ml please calculate allowable speed depending on the following formula Allow Speed rpm Maximum speedx 2 0 g ml Sample density g ml 11 1 4 Autoclaving A12 10P rotor is made of plastic cannot be high pressure sterilization
13. nti clockwise to decrease parameter values Press the button shift between speed and RCF display Speed area Lock status Time area sis X100rpm Figure 6 2 The main interface Main interface is as figure 6 2 The speed is set to be 4500rpm the door lock is released and the running time is 12 minutes When speed symbol 7Tis rotating this indicates the centrifuge is running If the rotation 1s faster the speed 1s higher Temperature of chamber 1s displayed and cannot be controlled Time symbol Odisplays the ratio of working to time setting The total time setting is divided into 10 sections 7 Rotor Preparation 7 1 Prepare the samples 7 2 Inject the samples into tubes AN CAUTION Oo Do not overload samples into the centrifuge which will cause leaking Do not exceed the actual capacity allowed in the user manual 7 3 Keep the tubes balanced o Although the centrifuge can accept sample balancing by eye we recommend that you keep this centrifuge in a well balanced condition to extend its life expectancy O Never intentionally run the centrifuge under an unbalanced condition even though the allowable imbalance 1s not exceeded 7 4 Inspect the rotor Check the rotor for corrosion or scratches before using CAUTION If any abnormality such as corrosion or scratches are found stop using the rotor and contact our service center Only manufacturer s rotors must be used with the unit CA
14. of the materials and procedure O To avoid electrical shocks insure hands are dry before handling the power cord or turning on off the power switch O For safety purposes do not enter within 30cm around this centrifuge when it is in operation Oo While the rotor is rotating never release the door lock O Unauthorized repairs disassembly or modifying the centrifuge except by our service center are strictly prohibited A CAUTION This centrifuge must be located on a firm and level table Make sure the centrifuge is horizontal before running Make sure the angle between the door and cover is greater than 70 degrees when opening the door Be careful not put your fingers or hands between the door and cover while the door is open Do not move or relocate the centrifuge when it is running If fluid spills in the rotor chamber please promptly clean and dry with a dry cloth to avoid sample contamination Ensure to remove any objects and fragments of the tubes dropped inside the rotor chamber before running the centrifuge Cautions with rotor 1 Always check for corrosion and damage on the rotor surface before using it Do not use the rotor if an abnormality is found 2 Do not set the speed beyond the allowable minimum speed of the rotor kits rotor and adapters Make sure to run it below the allowable maximum speed 3 Do not exceed the allowable imbalance 4 Use the rotor and tubes within their actual capacities 5 If the
15. ondition or practice which 1f not strictly observed or remedied could result in damage or destruction of the centrifuge NOTE Notes indicate an area or subject of special merit emphasizing either the product s capability or common errors in operation or maintenance Oo Do not operate the centrifuge in any manner not described in this User Manual When in doubt or have any troubles with this centrifuge ASK FOR HELP The precautions described in this User Manual are carefully developed in an attempt to cover all the possible risks However it is also important that you are alert for unexpected incidents Be careful operating this centrifuge A WARNING o This centrifuge is not explosion proof Never use explosive or flammable samples e Do not install the centrifuge in or near places where inflammable gases are generated or chemicals are stored e Do not place dangerous materials within 30cm of the centrifuge Prepare all necessary safety measures before using samples that are toxic radioactive or contaminated with pathogenic micro organisms Use of these is at your own responsibility o If the centrifuge rotor and accessories that have been contaminated by solutions with toxic radioactive or pathogenic materials clean it according to the decontamination procedure as specified O If you require service at site please sterilize and decontaminate the centrifuge in advance and then notify the service center the details
16. or service center 17 SCILOGEX LLC 500 Four Rod Road Suite 122 Berlin CT 06037 USA Tel 1 860 828 5614 Fax 1 860 828 5389 E mail info scilogex com Http www scilogex com
17. r firmly Avoid installing on a slippery surface or surface prone to vibration 2 Ideal ambient temperature is 20 C 5 C avoid placing the centrifuge in direct sunlight if temperature exceeds 30C 3 Keep clear of the centrifuge at least 10cm on both sides and at least 30cm behind it to guarantee the cooling efficiency 4 Keep away from heat or water to avoid sample temperature issues or centrifuge failures 4 2 Connection of the power cord and grounding AN WARNING O To avoid electrical shocks ensure your hands are dry when touching the power cord o This centrifuge must be grounded properly An minimum 10A outlet providing a sufficient ground is required and this must meet local safety requirements 5 Structure Door lock hook LCD Display Door release hole Operation panel Power switch Figure 5 2 Rear view of the centrifuge 6 Operation panel Figure 6 1 Operation panel I Select Select button Press the button to choose the program which you want to modify de The speed can be accelerated and held at the speed when Pulse button l pressing Pulse on Press the button to open the door The button is not available Hear Open lock button l l l when the centrifuge is running Press the button to start running The centrifuge will brake to ir Start Stop button o l l l stop running if pressed during centrifugation Clockwise rotate to increase program values Rotate O Parameter button a
18. splayed When the speed button is selected the speed symbol will flash the speed value The minimum speed value you can set 300rpm the minimum increment is 100 rpm O Rotate program button clockwise O to increase speed value Rotate the program button anti clockwise O to decrease speed value O You can speed up set the speed value by rotating program button O quickly There is a circulating function to increase decrease the speed values Rotate the program button clockwise O to change settings from small large maximum minimum Rotate the program button anti clockwise O to change settings from large small minimum maximum 2 Set the time SELEC Press select button time value flashes in the time setting mode Rotate the program button O to set running time from 30 seconds to 99 minutes When the time displays HD this 1s a continuous running mode 8 1 2 Start the operation 1 Press button to start running The door must be locked before rotor starts spinning The timer will start once the rotor starts spinning the screen displays the remaining run time 2 View and modify the operation programs Operation programs can be modified after the centrifuge reaches the set speed SELEC Pressing the select button returns the display to the program interface and displays setting SELEC programs Press the select button to the desired program When flashing rotate parameter button
19. y deform into an oval shape due to gravity 2 Remove locking nut and lid to prevent from deformation or rupture 3 Wait until the sterilizing chamber cools down to the room temperature before removing tubes 15 11 2 4 Conditions and life expectancy of tubes The life expectancy of plastic tubes depends on the characteristics of samples speed of the rotor used temperature applied and so on When the plastic tubes are used for ordinary aqueous samples pH between 5 0 and 9 0 their life expectancies are defined as follows Be operated at the maximum speed High quality tubes PA PC PP 30 50 operations Ordinary tubes PA PC PP around 10 operations Using in low speed can extend the tube life Life expectancy of tubes also depends on the pretreatment conditions such as cleaning and sterilization lifetime can be cut down Notice Do not use damaged or cracked tubes 12 Calculate RCF An RCF can be determined with the following calculation formula RCF 1 118xrxn x107 r rotating radius unit cm n rotating speed unit rpm 13 Ordering information Clinical Centrifuge with A12 10P rotor US plug 110V 240V 50Hz 60Hz Clinical Centrifuge with A12 10P rotor Cn plug 110V 240V 913123419999 DM0412 50Hz 60Hz Clinical Centrifuge with A12 10P rotor Euro plug 100V 240V 913223419999 DM0412 50Hz 60Hz Clinical Centrifuge with A12 10P rotor UK plug 110V 240V 913323419999 DM0412 50Hz 60Hz 91302341999
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