EOS Bravo and EOS Bravo Plus PRELIMINARY Service Manual
Contents
1. Tuta REN RS UM 4 sat kt TR RR TEF a LI 60 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 10 COOLING PLATE Power Switching MOTOR DRIVER Power Switching m RR t 1 E E F a 61 Hospitex Diagnostics Fig ure 11 Power Board LI a F Figure 12 CPU M PE PRR fs EFE LLLI ILLI 5 a 115111111744 F i HW LLLLLLILLLLLLIMM FERRER RRR mmn EOS Bravo and EOS Bravo Plus Service Manual 62 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 13 Power Board CPU Board 63 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 14 Internal PC 64 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 16 Level Sensor Board one F 65 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MECHANICALS Figure sue Mechanical group 2 c 1 rem 82. foes DEL 2 135194352 745 FEL ed 11411111 50 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 5 Analog Input Tae 79 C10 1uP 100K 27 P1 024 SORE e H XNT OUT 2828 pe RG 100K 1x 89922042 R40 100K 44 ET Va i i im AS 58 Pita 100K 1X a a 5213 do w pzn Ais 2K08 58 055 ye 9K08 1x lt 100K 2 41 2 Ete gt lt 1 CUL 013 B 184140 2000 51 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 2 POWER Board POS SEMS0R COMME Tie OME EVEL SENSOR EE T OPTICAL GRULDP PELTIER 7 RE HEAT GHH ES TIGH AMP VALVE FUN LEVEL SENSOR o T The POWER Board is the power interface between CPU and external devices like Stepper Motors Home Po
3. gt Qe 5 0021 S mm 2 lt 3 gt A 14 0245 e M m gt 4 05323 APR PAPA EAE S EN 0031 990 05323ME GHHHHHBO 4 nf 2 Nor t A nes LL lt Sees a 218 m DALLAS SNO i y oO j lliosiev 100 TERT cm d i 2 NONVOLATILESRAM SERIAL PORT i 05131 346882 gt e e Ce ax 3 488468 Figure 1 CPU Board The CPU Board is arranged with a Z80 processor family and its circuit A NON VOLATILE RAM contains the user defined parameters Two EPROMS are present and these contains the firmware During a firmware updates these components could be replaced 47 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 2 CPU Board layout E O8 CE se P7 187 Pa T __ o CE Ue EA us ET GC PC e yo 48 EOS Bravo and EOS Bravo Plus Se
4. SPEED IX STEPS L G ans nner Plate 1 1166 i x a E IY ee ee a bua MNT Tu im T oelmction F1 MOTOR ZERO Used fo execute the home position of the motor under examination F2 MOTOR FWD Used to move in The clock sense the motor under examination F3 MOTOR BWD Used fo move in The anticlock sense the motor under examination FA VALVE ON OFF Used to verify the functionality of the electrovalve situated on the diluter Used to verify the functionality of the two pumps in the double cuvette F5 DISPLAY INP command F5 active continues visualisation of the inputs 18 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F3 DIAGNOSTICS F3 HARDWARE TEST LEGENDA MOT Number relative to motor in object DESCRIPTION Description of the motor in object ENAB Command to block the motor in object FREE 1 BLOCKED SPEED Speed of the motor in object IX STEPS X Steps execute with the motor in object LOG POS Position were we want put the motor in object ABS POS Real position in steps that the motor has taken 19 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU gt F5 SERVICE gt F3 DIAGNOSTICS gt HARDWARE TEST _ LOGICAL POSITION INNER PLATE
5. LOOKING POWER LAMP CONNECTOR 20 regulate the lamp tension connecting the tester on the two pin of the cabling connector L1 present near to the lamp itself without disconnecting it and regulate the trimmer on the power board to obtain a value of the Tension equal to 45 65 Vcc 0 05 Caco POWER Board CPU Board 36 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 21 start the initial zero cycle of the instrument press the Enter key Since at this point some of the motors could be connected in a wrong way you can find a zero errors Ignore these errors pressing Enter key each time the cycle is interrupted and the instrument beeps to notify error At the end of the zero procedure you will find some screens which will notify the presence of errors in the methods ignore them by pressing STOP to arrive to the Main menu screen 22 adjust the rotation verse of the sample plate going to the hardware test screen pressing in sequence the keys F5 service F3 Diagnostics and again F3 hardware test starting from the main menu and evidence the line IP sample plate form the 5 table using the up and down arrows Press the key zero motor to execute research of the zero of the sample plate which should be stopped with the hole marked B in correspondence of the left side of the instrument Move the cursor in the column Pos Log by the key Enter and
6. fia 021 Sample needle offset 022 sample needle home position TOR 025 sample needle height before home for rotation over reagent bottles 024 Sample needle out of blot paper filter not for rotation 025 sample needle for sampling on internal 190 plate sample STD cups 026 Sample needle for sampling on internal 363 plate sample STD tube 027 Sample needle for dispensation on outer 243 plate 028 Sample needle for wash tubes on outer 243 plate 029 Sample needle for R2 R3 and bubbles 270 dispense on outer plate 030 sample needle on reagent bottles Ss si AN MOTOR rmi 034 Aspiration probe n 1 offset 035 Aspiration probe n 2 offset HESS Only for double cuvette 036 Aspiration probe high position in home pos 057 Aspiration probe wash step 1 038 Aspiration probe wash step 2 039 Aspiration probe sampling step for reading INESSE z PUMP MOTOR 043 Peristaltic pump wash step 1 044 Peristaltic pump wash step 2 045 Peristaltic pump aspiration step 3 cuvette 1 046 Peristaltic pump aspiration step 3 cuvette 2 047 Peristaltic pump mode el 0 Steps 75 msec only for double cuv 049 Rp motor position of bottle n 1 with rack 6 050 Rp motor position of bottle n 1 with rack 9 245 25 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 051 Rp motor steps between 2 bottles rack 6 052 Rp motor offset on reagent bottles 053 Rp motor idle p
7. EOS Bravo and EOS Bravo Plus Service Manual 2 008 1283 1765 1405 485 P 485 1219 1682 4348 1458 21 READING TEST Remove aspiration needle and put it in a holder with distillated water Press F3 ASPIRATE to aspirate After you have aspirate water select the channel that you want adjust example CUVETTEI and select the filter 1 340 nm You get visualized the reading of the filter in mV and in digit Using the trimmer of the gain we can increase or decrease value of the reading Act on gain trimmer to have a reading gt 1300 mV You should keep in mind that once you act on the trimmer of the gain to modify the reading of all the filters on board to the filters wheel To execute the regulation of the offset select the Filter O NULL and act on relative trimmer to have a reading gt mV Once the regulation of the gain and the offset is done it s necessary To execute an autozero to be able memorize new values THE OPERATIVE RANGE FOR EVERY SINGLE FILTER IS 500 mV 1800 mV 17 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F3 DIAGNOSTICS F3 HARDWARE TEST ee ee S HARDWARE TEST M Ww m mmy ae es 1 i mA i Lih um JEGCRIPTIOH
8. 094 sample needle pre heating duty cycle 095 Peltier element maximum power 096 Reaction bottle dead volume in microliters ae asl 098 OPPATH Optical path selection 7 Helma cuvette 10 099 Not used 100 Outer plate shake enable 0 0OzNO 1 YES 101 Outer Plate Home position check during the EA 055 execution run 102 Not used N 103 Not used 26 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 104 Reagent prime setup i os 105 Sample cup volume in uL 106 Number of execution tube washes 107 Not used 108 Machine cycle in sec 109 Schedule mode 110 Sampling probe wash after every sampling 0 02 NO 1 YES 111 Time out for data communication msec 112 Wait time on error during execution msec 113 Enable buzzer during editing 04 114 Else steps before needle stop in the reagent 7 bottle 115 LEVMOD Reagent level check 2 0 Disable 1 Normal RE 2 Normal Extrapolation 116 Not used 117 Conv Factor uL of reagent for sample 167 needle steps rack 6 118 Reagent level error in uL 119 Conv Factor uL of reagent for sample needle steps rack 9 120 Filter wheel position n 1 340 121 Filter wheel position n 2 0 Double cuvette 340 nm 122 Filter wheel position n 3 405 125 Filter wheel position n 4 Double cuvette 405 nm 124 Filter wheel position n 5 Double cuvette 492 nm 125 Filter wheel position n 6 Double cuvette 2 505 nm 12
9. The logical position from to 31 carries the relative cups under the relative point of sampling The logical position from 32 to 63 carries the relative cups under the relative point of sampling OUTER PLATE logical position from 1 to 96 carries the relative holes of the reaction segments under the aspiration needle The logical position from 101 to 196 carries the relative holes of the reaction segments under the point positioning of sampling arm The logical position from 1 to 189 carries the relative holes of the reaction segments under the aspiration needle The logical position from 201 to 389 carries the external ring of the reaction segments under the point positioning of sampling arm The logical position from 202 to 390 carries the internal ring of the reaction segments under the point positioning of sampling arm FILTER SELECT logical position corresponds to number of the relative filter PERISTALTIC PUMP NOT IN USE DILUTER SYRINGE logical position indicates uL to dispense REAGENT PLATE logical position from 1 to 18 or from 1 to 27 carries the sampling arm up to the relative bottles The logical position 41 sampling arm up the inner plate internal ring The logical position 42 sampling arm up to the inner plate external ring The logical position 43 sampling arm up to the outer plate The logical position 43 sampling arm up to the outer plate internal ring The logical position 44 sa
10. rm ke Central Mechanical group included Sampling Arm Reagent Plate Outer Plate Inner Plate Aspiration Needle 66 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 1 Sampling Needle Arm gt sampling needle arm movement and position is controlled by stepper motor driver 012 located onboard to the Power board that drives the stepper motor model 103 546 5342 and an optical sensor barrel that informs when the arm is at home position movement from the motor to arm is transferred by a mechanism driven from a belt and two pulleys Figure 2 05 __ Ihe Reagent plate movement and position is controlled by a stepper motor driver U11 located onboard to the Power board that drives the stepper motor model 23L C304 76W and an optical sensor barrel that informs when the arm is at home position movement from the motor to arm is transferred by a mechanism driven from a belt and two pulleys 67 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 4 Outer plate double cuvette he OUTER plate movement and position is controlled by a stepper motor driver U7 located onboard to the Power board that drives the stepper motor model 23L C304 76W and an optical sensor barrel that informs when the arm is at home Ihe movement from the motor to arm is transferred by a mechanism driven f
11. Bravo Plus Service Manual 31 verification of the two direct current peristaltic pumps only for double cuvette functionality using the screen hardware test pressing in sequence the keys F5 service F3 diagnostics and again F3 hardware test starting from main menu and press the key FA valve on off and on video we have the message VALVE 1 4 27 select 3 and verify that pump relative to the flow cell is moving in the clock wise direction At the end of this operation press another time 3 to leave the pump in release position select 4 and verify that the pump relative to the flow cell n 2 is moving in the clock wise direction At the end of this operation press another time 4 leave pump in release position 4 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 32 verification of the analogical entrance functionality going to the screen hardware test pressing in sequence the keys F5 service F3 diagnostics and again F3 hardware test starting from the main menu and then activate continuous visualisation of the entrance state pressing the key F5 entrance vis which will be visible on the upper right side of the screen Control that the number situated on the extreme right of the line with the digit CH O indicates a value tending to 420 and the one at the extreme right line with the digit Ch 1 indicates a value tending to 380 33 control that tension of the opti
12. Bravo Plus Service Manual Figure 3 PREAMPLIFIER Board monocuvette Figure 4 PREAMPLIFIER Board double cuvette 57 Hospitex Diagnostics Figure 5 INTERNAL PC Board XI xm p LT 1 a d TIN iu P 4 ETFRIHTER 4 EX E T EST ELEC as EOM ipri EAE TYO BEHRA Pris is LA s T SERIAL PORT 93232 T PU COMMECTION EOS Bravo and EOS Bravo Plus Service Manual e ETT XTwWEED JIRE TI 58 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 59 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 9 Main Transformer als if rder to connect the to 115 220 Select the main voltage on main transformer in o 115 VAC Connect the neutral and the 274 phase and the 4 ground together AC line has to be connect between 1 and 2 terminal 220 VAC Connect neutral and the 3 phase and the ground together AC line has to connect Between 1 and 3 terminal Figure 10 Main Transformer electrical Fat M tt
13. the rotation sense is effectively corrected 37 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 24 adjust the rotation verse of the filter wheel going to the screen hardware test pressing in sequence the keys F5 service F3 Diagnostics and again F3 hardware test starting from the main menu and then evidence line FS filter wheel from the motor s table using arrows up and down Press the key zero motor to execute a research of the filter wheel zero which should stop with the hole marked between the two arms of the transmission chain which is situated behind it Move the cursor the column pos log using the key Enter and then digit 1 the filter wheel should rotate in clockwise for more then one round positioning the hole marked 1 to the position where the hole marked was positioned before If This is not The case i e the filter wheel is moving in the contrary verse disconnect the connector of the cabling noted with MFS exchange between them the cables of blue colour reconnect connector and repeat this point from the beginning to verify that the rotation sense has been effectively changed 25 adjust the rotation verse of the peristaltic pump going into the screen hardware test pressing in sequence the keys F5 service F3 diagnostics and again F3 hardware test starting from the main menu and then evidence the line PM peristaltic pump of the motor s table using the arrows
14. then digit 10 the plate should rotate in anticlockwise for about 1 3 of round bringing the hole marked 10 into position where the hole marked B was before If this is not the case i e if The plate rotates in clockwise disconnect cabling connector with the note MIP exchange between them the two cables of blue colour and reconnect the connector and repeat this point from the beginning to verify that the rotation verse has been effectively corrected 23 adjust the rotation verse of the reaction plate going to the hardware test screen pressing in sequence the following keys F5 service F3 Diagnostics and again F3 hardware test starting from the main menu and then evidence on line test plate of motor s table using the keys arrows up and down Press the key Fl zero motor to execute the zero research of the reaction plate which should stop at the point marked in correspondence of the right side of the instrument Move the cursor of the column pos log using the key Enter and then digit 025 the plate should rotate in clockwise of about round bringing the point marked 2 into the position where the point marked 1 was situated before If this is not the case i e if the plate is moving in the opposite way disconnect the connecter of the cabling with the note MOP exchange between them the two cables of blue colour reconnect the connector and repeat the procedure of this point from the beginning to verify that
15. up and down Press the key F2 motor ahead and the reel of the peristaltic pump should rotate in anticlockwise sense for about 1 2 round If this is not the case i e reel is moving in the contrary sense disconnect connector of the cabling with the note MPM exchange between them the two blue colour cables reconnect the connector and repeat this point from the beginning to verify that the rotation sense has been effectively changed 26 adjust the movement sense of the dilutor using the screen hardware test pressing sequence the keys F5 service diagnostics and again F5 hardware test starting from the main menu and then evidence the line DS diluter of the motor s table using the arrows up and down Press the key Fl zero motor to execute a research of the piston zero of the diluter which should stop all in high without the instrument signing errors If this is not the case i e the piston stops all down and or an error is signed disconnect connector with the note MDS exchange between them the two blue coloured cables reconnect connector and repeat this point from the beginning to verify if the movement has been effectively corrected 27 adjust rotation sense of the reagent plate using the screen of hardware test pressing in sequence the keys F5 service F3 diagnostics and again F3 hardware test starting from the main menu and then evidence line RP reagent plate of the mo
16. you should verify that the water is regularly aspirated though needle and expelled the silicon tube Extract and immerge the needle into the water several times to see if the tube connected to it is emptied and filled each time In case there are some irregularities you should loosen and move pressing it more and less mobile part of the pump until you achieve a regular aspiration and or verify if there are any leakage or obstruction in the tubes or in the flow cell If the pump is still during the regulation press again the key F2 restart it Once you have obtained the requested regulation fix the blocking screw on the superior part to bring it near to the superior board of the mobile part 44 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 41 control the temperature indicated by the thermometer inserted the reaction plate which should show 42 0 02 Regulate the trimmer of the CPU board rotating it in anticlockwise if temperature is read is inferior or in clockwise if the temperature is superior CARIA 2929292250223 POWER Board CPU Board 42 control the temperature indicated by the thermometer positioned into the false flow cell which should be of 38 0 02 Regulate the trimmer of the CPU board rotating it in anticlockwise if the read temperature is inferior or in clockwise if it is superior 45 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Ma
17. 54 6 poles with the note MAN on the motor of the reading needle using a motor protection diode module 254 3 poles with the note SAN on the optical limit switch of the aspiration needle MX254 6 poles with the note MPM on the motor of the peristaltic pump using a motor protection diode module MX254 6 poles with the note on the filter wheel motor using a motor protection diode module MX254 3 poles with the note SFS on the optical limit switch of the filter wheel MX254 6 poles with the note FD1 on the cable coming form the preamplifier board 254 2 poles with the note 51 on the temperature sensor of the flow cell MX396 2 poles with the note PT1 on the Peltier cell of the flow cell thermostation MX396 2 poles with the note L1 on the optical lamp Faston couple coming out from the cabling together with the cables L1 and on security thermostate of the flow cell Flat 16 poles of the serial port on the cpu board to the serial port of the PC104 board 17 Take everything in high towards the electro valve the diluter piston rotating manually the command screw connected to motor tree SIEYNGE PISTON 18 turn on the instrument and make sure that within 1 2 seconds the printer executes an empty course of the head 33 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 19 Control that optical lamp is turned on looking at the hole present on the superior part of its support
18. 6 Filter wheel position n 7 Double cuvette 2 546 nm Filter wheel position n 8 Double cuvette 578 nm 128 Filter wheel position n 9 Double cuvette 630 nm HEROS 140 Methods structure type 199 Parameters checksum ie l Ls cm 28 UT rpm 21 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F5 LABORATORY DATA EDIT LARORATORY Dii im _ Lure F5 LAB DATA It allows to insert the data of the laboratory Furthermore it is possible to insert the name of the OPERATOR 0 Master and names of the other 4 OPERATORS with limited access the system 28 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 3 ASSEMBLY AND ADJUSTEMENT 1 3 4 9 position instrument with the door with the boards towards you assembly the CPU board fixing it with the four screws on the angles do not insert any connectors assembly the Power board fixing it with the four screws on the angles plus the central screw which is positioned between the connectors Jl and J2 Do not insert any connector or fuses Fix the fuses 5x20 mm of 3 15 A 6 3 for the alimentation at 110 Volt into the fuses holder o
19. ECUVETIE 25 AUTOZERO Used to execute an autozero of the complete filter wheel In case that during the autozero there are no variations respect the previous Values the cycle will be terminated without any signal 12 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F2 WASHINGS F5 AUTOZERO F5 AUTOZERO Example of autozero with a variation in the reading A variation of 5 is shoved with the visualization of the following voices ESC ABORT Cancels the session REPEAT Repeat the session of autozero ELSE ACCEPT Pressing any key except the ESC or Fl we accept the new value visualized EVERY TIME THE AUTOZERO GETS BLOCKED PRESS F1 TO RESTART THE PROCEDURE 15 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F2 WASHINGS F5 AUTOZERO 25 AUTOZERO Example of autozero with a problem In this particular case we have the lamp which is not functioning This can be noted since the values of all filters are very similar if not equal to the FILTER 0 OFFSET 14 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU gt F5 SERVICE gt F3 DIAGNOSTICS HUTGHATIC GNALYSER IMTERFACE READING TEST Used to execute reading tests in mV or execute the regulation of the offset and the gain F2 O D TEST Used fo
20. Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual EOS Bravo and EOS Bravo Plus LICG895 LICG892 LICG890 PRELIMINARY Service Manual HOSPITEX DIAGNOSTICS Via Provinciale Lucchese 145 50019 Sesto Fiorentino Florence Italy Tel 39 055 374083 Fax 39 055 374084 e mail hospitex hospitex it web http www hospitex com Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual INDEX 1 GENERAL VIEW 2 SERVICE MENU 3 ASSEMBLY AND ADJUSTMENT 4 ELECTRONIC AND MENCHANICAL DESCRIPTION Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 1 GENERAL VIEW Figure 1 Mono Cuvette Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 2 Double ROUF 5 SAMPUNG NEEDLE ARM REAGENTS Fate Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 3 External Devices connections mono and double cuvette A 1 a J amp Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 2 SERVICE MENU SERVICE ARAL WEE MAIN MENU gt F5 SERVICE WORKING PARAMETERS F2 MANUAL AND AUTOMATIC WASHINGS AUTOZERO F3 MECHANICAL AND FUNCTIONAL TESTS SYSTEM PARAMETERS ONLY FOR SERVICE F5 LABORATORY HEADING AND OPERATOR S NAME Hospitex Diagnostics EOS B
21. Not in use R Indicates state of the functioning of the heating circuit of the outer plate P Indicates state of the functioning of the heating circuit of the optical group 22 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU gt F5 SERVICE gt SYSTEM PARAMETERS gt PRESS THREE TIMES DOT 23 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 21 SELECT PARAM Select the parameter F2 NEXT PAGE Calls the next page F3 PREVIOUS PAGE Calls the previous page FA PRINT PARAM Prints the parameters to the selected page 25 CHANGE SIGN Changes the sign of the selected parameter NOTE THE ACCES TO THE PAGES OF THE SYSTEM PARAMETERS IS SUGGESTED ONLY FOR THE Hospitex Diagnostics PERSONNEL OR OTHER AUTHORISED PERSONNEL 24 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual N DESCRIPTION SAMPLE NOTE PLATE 32432 VALUE 000 Inner plate n position of external ring 001 Inner plate n position of internal ring 002 Inner plate offset for external ring 003 Inner plate offset for internal ring ERES ISESE _ OP MOTOR 007 Outer plate2 ring offset sample needle side Only for double cuvette 008 Outer plate ring offset sample needle side 009 Outer plate offset aspiration needle side 014 Filter selection offset Cal al Ea si DS MOTOR a 018 Diluter syringe conversion factor step 10ml Ss es 2 5
22. ation sense of the aspiration needle using the screen of hardware test pressing in sequence the keys F5 service F3 diagnostics and again F3 hardware test starting from the main menu and then evidence the line AN aspiration needle of the motor s table using arrows up and down Press the key zero motor execute a research of the aspiration needle which should stop all in high without the instrument is signing an error beep If this is not fhe case i e the aspiration needle is stops all in down and or an error is signed disconnect the connector of the cabling with the note MAN exchange between them the two blue coloured cables reconnect the connector and repeat this point the verify that the rotation sense has been effectively changed 39 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 30 verification of the diluter s electrovalve functionality using the screen hardware test pressing in sequence the keys F5 service F3 diagnostics and again F3 hardware test starting from main menu and then press key FA valve on off and on video we have message VALVE 1 4 7 select 1 and verify that the plastic cursor black and white present on the central part of the electrovalve of the diluter is moving correctly At the end of this operation press another time 1 to leave the cursor in release position ELECTROVALVE PLASTIC CURSOR 40 Hospitex Diagnostics EOS Bravo and EOS
23. bilizing tensions on the connectors J2 and J12 on the power board following the below scheme J12 pin 35 36 37 38 39 40 12 pin 33 34 4102 pin 31 32 12 25 20 27 28 29 30 4 5 6 8 ground J2 pin 7 J2pin 1 2 3 5 Voc acc of 49 to 5 1 5 of 49 To 5 1 5 1 Vcc acc of 5 0 to 5 2 Vcc variable of 5 2 to 6 5 12 0 VCC of 12440 126 9 for reagent cooling plate verify that supplementary fan situated beside the transformer is moving regularly without noise and that the air is directed towards outside of the instruments Connect the positive end of the tester on the pin 1 and negative end on the pin 2 of the alimentation connector of the reagent cooling plate and regulate the trimmer of the supplementary alimentator to obtain a tension between 14 01 and 14 02 Vcc Cooling Plate Power Switching o9c 9 9 oo op o 0 00 0 0 coooooo0o0o0on0o0 N eno o 9 9 0 0 ooo ww so 0 00 0 6000 0090020 0702020959 995969595 oo0000 0000 o o o o o o0 0020 0000 c o i o o immer PT Cooling Plate Power Connector 10 leave instrument on for some minutes and then re control again
24. cal lamp is not under the 5 60 V otherwise regulate it once again to arrive to value between the 5 60 Vcc and 5 65 Vcc 34 turn off the instrument put all the missing screws to complete the fixing of the power and CPU boards and fix the fan holder with the four screws TC M8x10 Connect the two fans to the connector of the cabling with the digit V1 and V2 MX254 2 poles 35 turn the instrument and verify that the two fans are moving regularly without noise and the air is sent towards power board 36 start the initial zero cycle of the instrument pressing the key Enter which should proceed and terminate without errors occur the end of the you should find main menu screen 42 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 37 position the thermometer on the reaction plate putting end of it in one of the reaction segments in the way that the edge of it arrived to the bottom near to temperature sensor and then dry with some paper tissues the holder to avoid the heat dispersion and contemporary block the thermometers position Take three reaction segments and insert them in the three holders remained free on the reaction plate REACTION SEGMENT 38 position a thermometer in optical group inserting end of it through the central hole of the false flow cell which should be inserted at the place of the true one in the optical group THERMOMETER 43 Hospitex D
25. d CPU Board 15 turn off the instrument and insert the connectors of the cabling on boards as follows 396 10 poles with the notes R2 EVI on the connector J3 of the power board 254 12 poles with the notes on connector J4 of the power board 254 12 poles with the notes MFS MPM on the connector J5 of the power board D 254 12 poles with the notes MDS MRP on the connector J on the power board 254 12 poles with the notes MSN MAN on the connector J7 of the power board 254 15 poles with the notes SIP SOP SFS SDS on connector J9 on the power board gt 254 15 poles with the notes SRP SSN on the connector J10 on power board 254 6 poles with the note FD1 on the connector J of the CPU board 254 6 poles with the note FD2 on the connector 7 of the CPU board Flat 16 poles on the serial port of the connector Jl of the CPU board J 20 poles of the internal PC on the connector J3 of the CPU board El m boos Power Board CPU Board 59 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 16 connect all the connectors of cabling on the dispositives present on the instrument according to the following scheme MX254 6 poles with the note MDS on the motor of the diluter using a motor protection
26. diode module MX254 3 poles with the note SDS on the optical limit switch of the diluter 254 2 poles with the note on the electrovalve of the diluter MX254 6 poles with the note MS on the motor of the sampling needle through the rotating connection using a motor protection diode module MX254 3 poles with the note SSN on the optical limit switch of the sampling needle through the rotating connection 96 2 poles with the note R2 on the heating reel through the rotating connection MX254 6 poles with the note MRP on the motor of the reagent plate using a motor protection diode module MX254 3 poles with the note SRP of the optical limit switch of the reagent plate MX254 6 poles with the note on the motor of the sample plate using a motor protection diode module 254 3 poles with the note SIP of the optical limit switch of the sample plate MX396 2 poles with the note R1 on the heating reel of the reaction plate 34 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 254 2 poles with the note ST2 on the temperature sensor of the reaction plate Faston couple coming out from the cabling together with the cables and ST2 on the security thermostate of the reaction plate MX254 6 poles with the note MOP on the motor of the reaction plate using a motor protection diode module MX254 3 poles with the note SOP on the optical limit switch of the reaction plate MX2
27. e hole used for the washings The instrument executes a number of XX washings in automatic The instrument executes a washing cycle of the sampling circuit The instrument executes a washing cycle of the aspiration circuit The instrument executes the autozero of all filters on board to the filter wheel Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F2 WASHINGS WASH POSITION WASH POSITION Used to select the number of hole used for the wash the example the First hole on the first reaction segment Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F2 WASHINGS F2 WASHES F2 WASHES Used to determinate number of XX complete washes of the hydraulic circuit SAMPLING ASPIRATION min 1 max 99 This is useful when we want to wash the hydraulic circuit very accurately but We don t want remain in front of The instrument 10 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU gt F5 SERVICE gt F2 WASHINGS gt F5 AUTOZERO MONOCUVETTE F5 AUTOZERO Used fo execute an autozero of the complete filter wheel In case that during autozero there are no variations respect the previous Values the cycle will be terminated without any signal 11 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU gt F5 SERVICE gt F2 WASHINGS gt F5 AUTOZERO DOUBL
28. execute readings in absorbance F3 HARDWARE TEST Used to execute tests of MECHANICAL ELECTRONICAL functionality FA PROCEDURE TEST Execute a complete test divided in single operation F5 Reserved key 15 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU gt F5 SERVICE gt F3 DIAGNOSTICS gt F1 READING TEST MONOCUVETTE 3 21 READING TEST Remove the aspiration needle and put it in a holder with distillated water Press F3 ASPIRATE to aspirate After you have aspirate water select the filter 1 340 nm You get visualized the reading of the filter in mV and in digit Using the trimmer of the gain we can increase or decrease value of the reading Act on gain trimmer to have a reading 1300 mV You should keep in mind that once you act on the trimmer of the gain to modify the reading of all the filters on board to the filters wheel To execute the regulation of the offset select the Filter NULL and act on relative trimmer to have a reading gt mV Once the regulation of the gain and the offset is done it s necessary To execute an autozero to be able memorize new values THE OPERATIVE RANGE FOR EVERY SINGLE FILTER IS 500 mV 1800 mV 16 Hospitex Diagnostics F1 348 F2 348 MAIN MENU F5 SERVICE F3 DIAGNOSTICS F1 READING TEST DOUBLECUVETTE
29. f net entrance module Connect the alimentation turn on the instrument and measure the exit tension of the transformer on the TR1 connector MX396 10 poles of the cablings according to the following scheme pin 1 lt gt 2 20 Vac acc of 20 to 22 V pin 3 lt gt 4 8 Vac acc of 8 to 9 V pin 4 lt gt 5 8 Vac acc of 8 to 9 V pin 3 lt gt 5 16 Vac acc of 16 to 18 V lt gt 7 11 Vac acc of 11 to 12 V pin 8 lt gt 9 5 5 Vac acc of 5 5 6 5 V 20 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual turn off the instrument and connect the connector of the cabling with the note 1 MX396 10 poles on the connector J1 of the Power board Power Board CPU Board WARNING form this moment avoid to position your face on the boards in particular the power board when the instrument is turned on 7 turn on the instrument and measure the stabilizing tensions connecting the negative end of the tester of the ground and the positive end on the exit point of the fuses holder on power board following the below scheme Fl2428Vcc acc of28 to 30 Vcc F2 7 Cdcc of T1o8 Vcc F3z2 4l5Vcc of 15 to 16 Vcc 11 of 11 to 12 Vcc of 11 to 12 Vcc Power Board CPU Board 30 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 8 measure the sta
30. iagnostics EOS Bravo and EOS Bravo Plus Service Manual 39 insert the peristaltic pump tubing loosening the screw blocking the mobile part of the pump in the way the vertical movement and make it go down the bottom Insert the free end of the silicon tube coming from the flow cell into the two holes present on the side of the mobile part Tear the tube from the exit hole until the two joining points of the tubes arrived to the mobile part SUPERIOR PART SILICON TUBE SILICON OR TEFLON TUBE MOBILE PART 40 regulate the aspiration of the peristaltic pump from the screen of hardware test pressing in sequence keys F5 service diagnosis and again F3 hardware test starting from the main menu and then evidence line PM peristaltic pump of the motor s table using the up and down arrows Move the cursor into the column p index and write the number 30000 Loosen blocking screw of the superior part in way to leave it free to move in a vertical way Press upwards the mobile part which should squeeze the silicon Tube in The way to make it arrive a distance of about 1 mm form pump reel and strengthen the blocking screw Insert the aspiration needle connected to flow cell and silicon tube coming out from the peristaltic pump into a recipient containing distilled water Press the key F2 motor ahead and the pump will move executing 150 complete rounds during which
31. mpling arm up to the outer plate external logical position 44 sampling arm up to repose condition SAMPLING NEEDLE logical position 3 sampling needle down in the bottom of sample cups The logical position 4 sampling needle down in hole of the reaction segments The logical position 8 sampling needle down in bottom of the reagents bottle 20 Hospitex Diagnostics ASPIRATION NEEDLE EOS Bravo and EOS Bravo Plus Service Manual the logical position 4 aspiration needle down in the bottom of the holes of the reaction segments the logical position 4 aspiration needle down in the bottom of the holes of the reaction segments external ring ONLY FOR THE DOUBLE CUVETTE 21 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F3 DIAGNOSTICS F3 HARDWARE TEST F5 DISPLAY INPUTS inr eb af gt iere 4 e IX CTE PS 1 Ol 2 MOLLSW Indicates the logical status of Sensor ques the logical status of the level sensor CHO Channel of the A D CONVERTER for the outer plate temperature Visualizes the value of temperature in teen degrees Channel of the A D CONVERTER for the cuvette Temperature Visualizes the value of temperature in teen degrees CH2 Not in use CH3 Not in use READING
32. nual 4 ELECTRONIC AND MECHANICAL DESCRIPTION Echo instrument can be divide in two different parts Electronic part Mechanical part The electronic part included Board Power Board Preamplifier Board Internal PC Board Level Sensor Board Main Transformer Cooling Plate Power Switching Motor Driver Power Switching The Mechanical part included Central Mechanical group Optical group Diluter group Peristaltic Pump group 46 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Electronic INTERNAL PC POWER PHOTODIODE 1 PLATE TEMP SENSOR INTERNAL PRINTER OPTICAL GROUP TEMP SENSOR POWER SIGNAL PHOTODIODE 2 a jet n ee e 9 14 245 54 200 05323 6 NI Hoa0031 25 777 04611 72 220 LT XT MPOWER BOARD O 1 REN 04511 t 0027 We e Hen de e de de A d Fe B 5 23 Has0031 S 53 245 d 05323
33. osition 054 Rp motor inner plate external ring 055 Rp motor inner plate internal ring 056 Rp motor outer plate dispensing position 057 Rp motor offset on inner outer plates ES 058 Rp motor outer plate dispensing position 059 Else steps before reagent plate arm mechanical stop 060 Reagent rack selection 18 reagents or 27 reagents ases xj _________ MOTOR SPEED 062 Inner plate rotation speed sari 064 Outer plate rotation speed 065 Outer plate shake speed SS 067 Filters wheel rotation speed U 069 Peristaltic pump 1 rotation speed 070 Peristaltic pump 2 rotation speed Only for double cuvette 071 Diluter motor up speed 072 Diluter motor down speed 073 Not used 075 RPVEL Reagent plate arm rotation speed lerne 077 Sampling needle up speed 078 Sampling needle down speed 079 Aspiration needle up down speed per EY VARIOUS 082 Current software release 083 Serial port mode 0 Host 1 2 Graph consolle 084 Host communication baud rate 085 Consolle printer 0 O Int 1 ext 086 Execution printer 0 O Int 1 Loc ext 087 Execution printer results field delimiter 088 DATEEN Automatic data amp time from PC D O No 1 Enable date from PC ort DESI 2 Enable Date amp Time 089 Not used os 090 Keyboard automatic setting 2910 091 Not used 092 Outer plate temperature 10 093 Cuvette temperature SOS
34. ravo and EOS Bravo Plus Service Manual MAIN MENU F5 SERVICE F1 USER PARAMETERS WASHING STEPS ASPIRATION STEPS OUTER PLATE TEMP CUVETTE TEMP ENABLE REACTION SHAKE PRE HEATING DUTY CYCLE PRINTER MODE AUTOMATIC DATE Peristaltic pump 5 steps relative to the first and the second phase of aspiration used to wash the cuvette with liquid and air Peristaltic pump s steps used fo aspirate remain of the liquid from the cuvette Parameter to modify the Temperature of the plate which holds the reaction segments Parameter to modify the temperature of the cuvette Parameter to enable the function of shake of the plate which holds the reaction segments CO s NO Le YES Parameter relative to the power impulse relative to the resistance that is situated around the heating reel Parameter relative to the printing ALL THE PRINTING ARE MADE ON THE INTERNAL PRINTER 1 THE PRINTINGS OF THE SESSION ARE MADE ON THE INTERNAL PRINTER AND THE PRINTINGS OF THE REFERTS ARE MADE ON THE EXTERNAL PRINTER 1 1 THE PRINTING ARE MADE ON THE EXTERNAL PRINTER Parameter relative the date and time from the internal PC 0 NO 1 ONLY DATE 2 DATE HOUR Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual MAIN MENU gt F5 SERVICE gt F2 WASHINGS Fl WASH POSITION F2 WASHES F3 TUBES WASH F4 CUVETTE WASH F5 AUTOZERO Used to select the number of th
35. rom a belt and two pulleys 68 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 5 Inner Plate TN m E k INNER plate movement and position is controlled by a stepper motor driver 06 located onboard to the Power board that drives the stepper motor model 23L C304 76W and an optical sensor barrel that informs when the arm is at home position movement from the motor to the arm is transferred by a mechanism driven from a belt and two pulleys 69 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 6 Aspiration Needle Arm mono cuvette f S Figure 7 Aspiration Needle Arm double _ EIEI e 2 ENT gt 4 a p ao 4 xi ys T il ASPIRATION NEEDLE ARM movement and position is controlled by a stepper motor driver 013 located onboard to the Power board that drives an stepper motor model 103 546 5342 for mono cuvette or an stepper motor model 103 547 52500 for double cuvette and an optical sensor barrel that informs when the arm is at home position movement from the motor to the arm is transferred for the mono cuvette by a mechanism driven from a belt and two pulleys movement from the motor to two arrns is transferred for the double cuvette by mechanism driven 70 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Se
36. rvice Manual E Figure 7 Optical group mono cuvette m AN j L double cuvette _ EST m T Hy Elle The FILTER WHEEL movement and position is controlled by a stepper motor driver U8 located onboard to the Power board that drives an stepper motor model 23LM C304 76W for mono cuvette an stepper motor model 103 547 52500 double cuvette and an optical sensor barrel that informs when the arm is at home position The movement from the motor to the wheel is transferred for the mono cuvette by a mechanism driven from a belt and two pulleys The movement from the motor to the wheel is transferred for the double cuvette directly 71 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 10 Diluter he SYRINGE movement and position is controlled by a stepper motor driver U10 located onboard to the Power board that drives an stepper motor model HY200 1713 0100 and an optical sensor barrel that informs when the syringe is at home position movement from the motor to the syringe is made by a mechanism driven from a no ending screw 12 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure 13 Peristaltic pump Cmono cuvette ES 2 PERISTALTIC PUMP movement is controlled by a stepper motor driver 09 located onboard to the Power board that drives an
37. rvice Manual Hospitex Diagnostics icroprocessor and MI Figure 3 CA IT 491 3 10 24 TI LLEI 1 48 oo 4 88883885 8 524124455442 WW 000 som veni iii ipm m 222 RARI ded 14 aon Oars 0114 d NOIRS 1111 lt ri Tm cles J vico eror dod vis c4 5 5 45244 ape gt 1 1117 2 17 1 4 0 1 4 XD D 4 C CI di id gt wu g 8 o 49 EOS Bravo and EOS Bravo Plus Service Manual Hospitex Diagnostics Digital INPUT OUTPUT Figure 4 LEL PETER T M m E Hii bes BaB33835 eel ee T 22133135 u2323385 Bie duct E 15855335 ATIT
38. sition sensors Temperature sensors and so on The Power Board receive AC voltage from the Main Transformer and provides DC power supply 12 Vcc and 5 Vcc to the instrument electronic Fuses are present on the Power to protect the parts in case of malfunction Fuses Values 10A 6x30 2 5 6x30 F3 10 6x30 F4 200 5x20 F5 200 5x20 No adjustment are necessary for the DC voltages because integrated stabilizer C are used 22 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure POWER Board Layout fen an et ant ET pm Ga 53 Hospitex Diagnostics Figure Power circuit Pes EOS Bravo and EOS Bravo Plus Service Manual TH TM 54 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure Motor Drivers circuit m 5 2 E d l 1201 2 SIE a DLL nm ES ur 1 mrs VETE m m s Lu LIN gt Li a _ CILE 55 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual Figure Input and Output circuit 56 Hospitex Diagnostics EOS Bravo and EOS
39. stepper motor model 23LM C304 76W movement from the motor to the rotor is direct a i p 5 LI a t ul unm uud au ar x Figure 14 Peristaltic pumps E PERISTALTIC PUMPS movement is powered directly from the Power board that drives two direct current motor 12 VCC ASF Thomas 73
40. the tensions as point 7 and 8 and verify that there are no problems due to non correct assembly of the boards 31 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 11 turn off the instrument and insert the connectors of the cablings on Power and CPU board Cc DOXCJCCUE as follows Power Board CPU Board MX396 8 poles with the notes VID V2 L1 on the connector J2 of the power board 254 8 poles with the notes 511 572 on connector J10 of the CPU board 12 take the two board interconnection cables and insert them as a bridge between the connectors CPU lt gt J12 power CPU lt gt J11 power i LLLLLPPTTT 1411 Phere TTET 171711 32 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 13 tum on the instrument 14 Check the two reference tensions VREFI e VREF2 on the CPU board as follows VREFI connect the negative end of the tester on the analogical ground and the positive end on the pin 2 of the U26 and then regulate the trimmer P1 to obtain a value of tension equal to 1 000 0 005V VREF2 connect the negative end of the tester on the analogical ground and the positive end on the pin 6 of the U30 and then regulate the trimmer P2 to obtain a value of tension equal to 2 731 Vcc x 0 005V Ground CI Power Boar
41. tor s table using the arrows up and down Press the key F1 zero motor to execute a research of the reagent plate zero which should stop with the needle arm in correspondence of the side of the instrument without the instrument is signing an error If this is not the case i e the plate is rotating in the contrary sense and or an error is signed disconnect the connector of the cabling with the note MRP exchange between them the two blue coloured cables reconnect the connector and repeat this point the verify that the rotation sense has been effectively changed 38 Hospitex Diagnostics EOS Bravo and EOS Bravo Plus Service Manual 28 adjust the rotation sense of the sampling needle using the screen of hardware test pressing in sequence keys F5 service F3 diagnostics and again F3 hardware test starting from the main menu and then evidence the line SN sampling needle of the motor s table using the arrows up and down Press the key zero motor to execute a research of the sampling needle which should stop all in high without instrument is signing an error beep If this is not the case i e sampling needle is stops all in down and or an error is signed disconnect connector of cabling with the note MSN exchange between them the two blue coloured cables reconnect the connector and repeat this point the verify that the rotation sense has been effectively changed 29 adjust the rot
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