operation (continued)
Contents
1. 37 99 R 38 I CTO CT AUN ili ieri TR ENT 38 PONOT ae e AEE EA A EE iii 38 PARTS AND CONSUMABLES ana iiiaae 39 3 Page LAB INTRODUCTION Thank you for purchasing a Sheldon SCO Series Air Jacketed CO Incubator We know that in today s competitive marketplace customers have many choices when it comes to constant temperature equipment We appreciate you choosing ours Our continued reputation as a leading laboratory product manufacturer rests with your satisfaction Sheldon Manufacturing Inc stands behind our products and we will be here if you need us These CO incubators are intended for professional industrial or educational cell cultivation applications They are not designed for use in hazardous or household locations Before using the incubator read the entire manual to understand how to install operate and maintain the incubator in a safe manner Keep this manual available for use by all incubator operators Ensure that all operators are given appropriate training before the incubator begins service GENERAL SAFETY CONSIDERATIONS Note Failure to follow the guidelines and instructions in this manual may create a protection impairment by disabling or interfering with the unit s safety features This can result in injury or death Your Shel Lab CO incubator and its recommended accessories are designed and tested to meet st2. Replace the disk shaped in line HEPA filter in the CO tubing between the supply cylinder and the incubator at least once per year The filter is directional Always make sure the side stamped IN is plumbed facing the supply cylinder or wall source ELECTRICAL COMPONENTS Electrical components do not require maintenance If the incubator fails to operate as specified please contact your Shel Lab Dealer or Sheldon Technical Support for assistance please see page 5 35 Page LAB MAINTENANCE CONTINUED CONDENSATION AND THE DEW POINT Relative humidity inside the incubation chamber should never be allowed to exceed 80 at 25 C Exceeding this thresholds will likely result in condensation possible leaks around the incubator and may cause corrosion damage if allowed to continue for any significant length of time Condensation will appear wherever the humidity level in the incubator chamber reaches the dew point The dew point is the level of humidity at which the chamber atmosphere cannot hold more water vapor The warmer the air the more water vapor it can hold As the level of humidity rises in an incubation chamber condensation will first appear on surfaces that are cooler than the air temperature Near the dew point condensation will form on any item or exposed surface that is even slightly cooler than the air When the dew point is reached condensation forms on nearly all exposed surfaces Managing condensatio
3. Temperature Display and Controls Marked SET TEMPTERATURE the incubation chamber temperature control panel comes with a green digital display that shows the air temperature within the chamber accurate to 0 1 C The display also shows the user selected temperature set point and can be used to enter offsets during temperature calibrations The controls consists of an UP DOWN arrow pad for inputting set point temperatures and performing calibrations Red LED indicator lights marked HIGH and LOW illuminate whenever an alarm condition associated with the chamber s temperature is reached The yellow LED marked MUTE illuminates whenever an audible alarm is being muted Heating Activated Light The green pilot light labeled HEATING ACTIVATED illuminates whenever the incubator heating element is energized 18 Page LAB CONTROL PANEL OVERVIEW CONTINUED i Over Temperature Limit Thermostat OTL This control is marked SET OVER TEMPERATURE or Set Over Temp Safety and is equipped with a graduated dial The OTL is a mechanical backup system that operates independently of the digital temperature controller It guards against a failure of the digital controller that would allow the chamber temperature to rise past the controller set point Please see the Over Temperature Limit System description in the Theory of Operations section page 21 for a more complete explanation OTL Light The red pilot light marked OVER TEMPERATURE ACTI
4. CONTINUED MUTING THE AUDIBLE CO ALARM Visual high and low deviation indicator alarms will activate if the incubator s CO level deviates 1 above or below the CO set point An audible alarm will sound immediately for a high deviation The low deviation audible alarm will sound after the visual low indicator alarm has been continually illuminated for fifteen 15 minutes This delay prevents the alarm from sounding whenever a door opening creates a short lived drop in gas concentration 1 To mute an audible alarm press and hold the Up or the Down arrow button for one 1 second on the CO Control sub panel 2 A muted alarm will stay silent for the duration of the current temperature deviation and the amber Muted indicator will remain illuminated No GAS SUPPLY ALARM NGS If the Low Gas deviation indicator is active for longer than twenty 20 minutes a second alarm will activate The letters ngS will appear in the CO display to indicate No Gas Supply This alarm will remain active even if the incubator is turned off and turned back on The NGS Alarm will remain on until CO is restored to the chamber It may require several minutes of CO inflow to achieve a concentration high enough to deactivate the alarm Always check the level of the CO gas supply cylinder or wall source if a No Gas Supply alarm is active Deviation 15 20 CO Start Minutes Minutes SetPoint 4 1 or lower Low Visual Alarm Audible N
5. Supply to the Incubator iii 13 CW 14 Cleaning and Deionized Water ire 15 GRAPHICSYMBOES E 16 CONTROL PANEL OVERVIEW aires cicale iis UE P GE 18 OP RATION M 20 I O Ae NSo uc m 20 ui oisinelie PiseUe iem T Q 22 r an EIF e cEeqa m 23 Calibrate the Temperature display ss 24 Setihe Over Temperature LIMIT nier tte ERE n boo EIER EA E EE RAE HEAR aiar 26 Muting the Audible Temperature Alarm inserer 27 SOL DO Oe Toce NM C T 27 CANO CO DISPIAV iris 28 Muting the Audible CO2 Alarm ucccccsssecccccccsnececececaueceecesnseueceessaaeneeesssaaeseessssaseeeessasaeaeeesssaaageeesssaaagseesssaaes 31 No Gas Supply Alarm NGS mE 31 LOSING INCI ili 32 Attaching equipment to the Interior Accessory Outlets eene 32 Data Output Capabilities iii liana 33 USER MAINTENANCE e 34 Cleaning and ISIN eee PME ni 34 Maintaining Atmospheric Integrity iris 35 E GGHIGA CONDONI Fc NNI RHET 35 Condensation and the Dew Point eiiis essen hannes nhan anna snas asas nass aa sans n ssa annes 36 algo ege peu cc 37 VE T m 37 Buh ce eet 37 CADI
6. please contact your Shel Lab dealer or distributor for assistance CONTACTING ASSISTANCE If you are unable to resolve a technical issue with your incubator please contact Sheldon Technical Support Phone hours for Sheldon Technical Support are 6am 4 30pm Pacific Coast Time west coast of the United States UTC 8 Please have the following information ready when calling or emailing Technical Support the model number and the serial number see page 10 EMAIL tech shellab com PHONE 1 800 322 4897 extension 3 or 503 640 3000 FAX 503 640 1366 Sheldon Manufacturing INC P O Box 627 Cornelius OR 97113 5 Page LAB RECEIVING YOUR INCUBATOR Before leaving our factory all SCO Incubators are packaged in high quality shipping materials to provide protection from transportation related damage When the unit departs the factory safe delivery becomes the responsibility of the carrier Damage sustained during transit is not covered by the incubator warranty This makes it important that you inspect your SCO Incubator for concealed loss or damage to its interior and exterior when receiving it If you find any damage to the unit follow the carrier s procedure for claiming damage or loss The orientation photos on the following pages may serve as a useful visual guide for inspections INSPECTING THE SHIPMENT Carefully inspect the shipping carton for damage Report any damage to the carrier service that delivered
7. Calibration procedures Turn the Over Temperature Limit control dial clockwise to the maximum position e Press either the Up or Down key one time on the Temperature Control panel to activate the temperature set point mode The temperature display will briefly flash the letters SP to indicate a Set Point is about to be displayed The Set Temperature digital display will then dim and start to blink showing the adjustable temperature set point Use the Up and the Down key to adjust the set point to your application s or procedure s set point If neither key is pressed within 5 seconds the Set Temperature display will stop blinking and return to displaying the current temperature of the incubator Wait 5 seconds after entering your set point The display will stop flashing and the set point is now saved in the microprocessor controller The incubator will now heat or cool automatically adjust to match your set point Leave the Over Temperature Limit control at the maximum setting if you will be calibrating the temperature display as part of the Preparing the Incubator procedure 23 Page OPERATION CONTINUED CALIBRATE THE TEMPERATURE DISPLAY This procedure requires a temperature reference sensor device Always use a reference device calibrated to at least 0 1 C For best results use a digital device with a wire sensor probe Temperature calibrations are performed to ensure that the incubator temperature display matches the
8. Heating and cooling ducts or other sources of fast moving air currents e High traffic areas e Direct sunlight POWER SOURCE Note Electrical supply to the incubator must conform to all national and local electrical codes Always position the unit so that the end user has access to the power cord When selecting a location for an SCO incubator verify that the earth grounded wall power source matches the voltage and ampere requirements listed on the incubator data plate Supplied voltage must not vary more than 10 from the data plate rating Damage to the incubator may result if supplied voltage varies more than 10 Use a separate circuit to prevent loss of product due to overloading or circuit failure These incubators are intended for a 50 60 Hz application at the following amperages SC058 SCO40 SCO31 15 Amps 15 Amps 14 5 Amps If the requirements match the power source plug the power cord into the earth grounded outlet These units are provided with an 115V 2 5m NEMA 5 20P power supply cord 11 Page LAB INSTALLATION CONTINUED LIFTING A SCO Incubator should only be lifted by its bottom surfaces using proper heavy lifting machinery such as a forklift or pallet jack Handles and knobs are inadequate for lifting or stabilization The unit should be completely restrained from tipping during lifting Transporting the unit while lifted is not recommended and may be hazardous Remove all moving parts such as shelves a
9. Over Temperature Limit system Indique le syst me de d passement de temperature Indicates AC Power Rep re le courant alternatif Indicates I ON and O OFF rep re de la position MARCHE de l interrupteur d alimentation O rep re de la position ARR T de l interrupteur d alimentation Indicates protective earth ground Rep re terre lectrique Indicates UP and DOWN respectively Touches de d placements respectifs vers le HAUT et le BA Indicates Manually Adjustable Indique un bouton r glable manuellement y o u amp 16 Page LAB JQ F GRAPHIC SYMBOLS CONTINUED Symbol Definition Indicates Potential Shock Hazard Signale danger lectrique WEEE Directive compliant logo TON Indicates the unit should be recycled Not disposed of in land fill Indique l appareil doit tre recycl Ne pas jeter dans une d charge Indicates CO Gas Indique gaz CO2 Indicates carbon dioxide gas content as a CO Indique le gaz carbonique contenu en 17 Pa U CD 0a D LAB CONTROL PANEL OVERVIEW SET OVER TEMPERATURE SET TEMPERATURE Set Temperature C Set CO2 High Mute Activated Injecting C O2 SERIES Sneidon Manufacturing Inc Power Switch The main power switch on the control panel controls all power to the unit and its systems The switch illuminates when in the on position on the SCO58 A green pilot light labeled Power On illuminates on the SCO40 and SCO31
10. actual air temperature inside the incubation chamber Calibrate as often as required by your laboratory protocol or regulatory compliance Sheldon Manufacturing strongly recommends performing a temperature calibration when installing the incubator in a new environment Allow the incubator to stand for at least 8 hours undisturbed prior to this procedure running at its operational set point in order to stabilize with no temperature fluctuations greater than 0 1 C Failure to allow at least 8 hours will result in an inaccurate temperature calibration If you have not already done so place the temperature sensor of the Note Sensor probes may reference device inside the incubator as close as possible to the be introduced into the chamber s geometric center Check that the sensor is not in direct contact chamber through the door with the shelving or on the SCO58 through the access port Place the port stopper back into the port after introducing the probe Note A thermocouple sensor probe s sleeve may be taped to the shelving as long as the exposed copper end is 2 inches 5cm above the shelf An exposed sensor probe in direct contact with the shelving may experience heat sinking which can result in an inaccurate temperature reading 2 Allow the temperature to re stabilize if the door has been opened a The reference device temperature measurement should not change for at least one 1 hour in order for the chamber to be considered r
11. seconds a The display will brighten and cease flashing b Your set point is now saved in the CO controller and the incubator will now begin adjusting to reach the set point 27 Page LAB OPERATION CONTINUED CALIBRATING THE CO DISPLAY Note The CO display and sensor come calibrated from the factory to a 5 concentration at 179 Feet 54 meters above sea level The calibration procedure verifies the accuracy of the CO reading and corrects for any measurement errors Calibrate as often as required by your laboratory or production protocol or regulatory requirements Sheldon Manufacturing recommends calibrating the CO display whenever the SCO Incubator is installed in a new environment Always use a CO reference sensor regularly calibrated to a concentration accuracy of 0 1 For best results use a digital gas analyzer with a sample collection tube The accuracy of the calibration process is limited by the accuracy of your reference device i uu mple Port Location The incubator should be calibrated at the CO concentration level it will be set to GO empleo octo during your incubation application or process Wait until the unit has stabilized at its CO set point before starting the calibration procedure Stabilized means there have been no changes in concentration on the unit s CO display for 1 hour while the unit is sitting undisturbed Failure to wait until the unit is stabilized prevents an accurate
12. tubing kit between the gas source and the incubator The filter is directional Make sure that it is installed correctly with the side stamped IN facing toward the supply source SCO Incubators use only small quantities of CO Precise regulation of gas input is vital for the incubator s performance Always use a two stage CO pressure regulator Some single stage regulators have 2 gauges Make certain your regulator is a two stage regulator Set the CO regulator within the operational range of 15 25 Pounds per Square Inch PSI Do not exceed 25 PSI PSI Megapascals Kilopascals Bar 15 25 PSI 0 103 0 172 Mpa 103 42 172 37 Kpa 1 03 1 72 bar Please see the Pressure Units Conversion table on page 38 in the Unit Specifications section for the formulas for converting PSI into other units of pressure measurement 13 Page LAB INSTALLATION CONTINUED SHELVING INSTALLATION Shelves should be installed evenly spaced in the incubation chamber to obtain the best temperature uniformity For ease of installation Sheldon Manufacturing recommends that two people lift and install each shelf in the SCO58 Incubator 1 Squeeze each shelf clip when installing Insert the top tab first into shelf mounting standard and then the bottom tab using a rocking motion a SCO58 install six 6 clips for each shelf Two 2 each on the left right and back walls of the incubation chamber b SCO40 SCO31
13. AIR JACKETED CO INCUBATORS 115 Volts SHEL Installation and Operation Manual SCO58 SCO40 SCO31 Previously designated 2460 2440 2428 AIR JACKETED CO INCUBATORS 115 Voltage Installation and Operation Manual Part Number Manual 4861710 Revision December 24 2014 Pictured on front cover SCO58 SCO40 SCO31 These units are compliant with the following standards for use within an ambient air pressure range of 22 14 31 3 inHg 75 106 kPa with no flammable volatile or combustible materials being heated CAN CSA C22 2 No 61010 1 2012 CAN CSA C22 2 No 61010 2 010 R 2009 UL 61010A 2 010 2002 UL 61010 1 2012 EN 61010 1 2010 EN 61010 2 010 2003 2 Page LAB TABLE OF CONTENTS INFRODUGTION iii 4 General Safety Considerations sine 4 Engineering Improvements iii 5 COAG FUSS SIMIC MNT 5 RECEIVING YOUR ING UB AT OP ER ns aa a a aAa 6 Inspecting the SIDI CIE 6 Recording Data Plate Information vi nasetlcsvnianinsiinus eun adum i hohe rei nA a ma UR enki EE REM a Dr dd 10 INSTALLATION m e M Ye wes 11 ATOE CONO OE loi ri iii 11 POCA ON m 11 OO ee M 11 Bg des ae etes te ta a a ei 12 OI FIA 12 ACCESS Fon DODO iaia 12 Connect the CO
14. Bottom Incubation Chamber Power Inlet Circuit Breaker and Data Plate Door Gasket Incubator Door me 9 Page RECEIVING YOUR INCUBATOR CONTINUED RECORDING DATA PLATE INFORMATION sHEL BON WANUFACTURING Inc lt 300 N H The data plate contains the incubator model number and serial C3 Www SHELLAB com number Record this information for future reference On the SCO58 the data plate is located on the back of the unit on MODEL Ni the top right side On the SCO31 and SCO40 the data plate is toward the bottom and back of the unit s right side Data Plate Date Plate Information Model Number Serial Number 10 Page LAB INSTALLATION AMBIENT CONDITIONS The SCO Incubators are intended for use indoors at room temperatures between 15 C and 30 C 59 F and 86 F at no greater than 80 Relative Humidity at 25 C 77 F Allow a minimum of 4 inches 10cm between the incubator and walls or partitions and 2 inches 5cm of clearance above the top of the incubator for unobstructed airflow Operating the unit outside of these conditions may adversely affect the temperature range and stability LOCATION When selecting a location to install the incubator consider all environmental conditions that can affect the unit s effective temperature range uniformity and stability For example e Ovens autoclaves and any device that produces significant radiant heat e
15. TERNET http www Shellab com PHONE 1 800 322 4897 503 640 3000 FAX 503 640 1366
16. VATED or Over Temp Safety Activated illuminates when the Over Temperature Limit system takes control of the incubator It do so by overriding the controller board through depowering the heating element Under normal operating conditions this pilot lamp should never turn on CO Control and Display Panel of CO Labeled SET CO this panel includes a green digital LED display connected to an infrared sensor which shows the concentration of CO in the incubaion chamber as a percentage of the chamber atmosphere The UP DOWN arrow pad is used to input a CO2 concentration level set point for the chamber and to enter calibration corrections Red LED indicator lights marked HIGH and LOW illuminate whenever an alarm condition associated with the chamber CO level is reached The yellow LED marked MUTE illuminates whenever an audible alarm is being muted CO Injection Light C Marked CO INJECTING this pilot light illuminates during injections of CO into the incubation chamber air stream Circuit Breaker Each SCO Incubator comes equipped with a circuit breaker located adjacent to the power inlet If the breaker trips set the unit power switch to off and locate a cause for the overcurrent condition before resetting the breaker The circuit breaker in the SCO58 and SCO40 is a 20 amp breaker In the SCO81 it is a 15 amp breaker 19 Page LAB OPERATION THEORY OF OPERATION Heating and CO Control The incubator uses a
17. ate at your selected temperature and CO set points The Over Temperature Limit System OTL When set the OTL system prevents runaway heating in the event of a failure of the microprocessor controller board or its thermometer probe by depowering the heating element whenever the temperature in the incubation chamber exceeds the OTL setting Typically the OTL is set 1 C above the temperature display set point Because of its nature as a mechanical cutoff system and its lack of PID analytics the OTL cannot deliver the same degree of temperature stability and measurement precision as the digital display and microprocessor board The OTL System should only be used as a means of heating regulation for the incubation chamber until a failed controller board and its thermometer probe can be repaired or replaced 21 Page LAB OPERATION CONTINUED PREPARING THE INCUBATOR Perform the following steps and procedures to prepare the incubator for use each time it is installed in a new location 1 2 Note Clean and disinfect the incubator chamber Verify the workspace power supply and incubator data plate requirements match SCO58 only Verify that the side access port plug is in place You may play the sensor probe of a temperature reference device inside the incubation chamber at this time a Doing so saves time during the Temperature Calibration procedure by eliminating wait times for the chamber temperature to re stabilize a
18. ature Display will cease flashing and store the last displayed value as the new current chamber temperature value 6 After correcting for the offset wait five 5 seconds a The incubator s Temperature Display will cease flashing and store the correction b The incubator will now begin heating or allow itself to cool in Set Temperature C High Mute n BENI Set Temperature C High Mute i BE T Set Temperature C High Mute i Low order to reach your set point with the corrected display value A v Wait 5 Seconds Set Temperature C 7 Allow the incubator sit for one 1 hour undisturbed to stabilize Hon after it has achieved the temperature set point Failure to wait until Mute E the unit is fully stabilized will result in an inaccurate reading See next La a e Wait 1 Hour step 3 mm uu 8 Compare the reference device reading with the incubator temperature LU display a Ifthe reference device and the incubator temperature display AI readings are the same or the difference falls within the range T n of your laboratory protocol the incubator is now calibrated Dm for temperature A v 313 9 If the two readings are not the same or fall outside your laboratory protocol repeat steps 5 8 Set Temperature C High Mute i Low Procedure continued on next page 25 Page LAB OPERATION CONTINUED Temperature Calibration Continued 10 If the temperature readin
19. ay of fifteen minutes This delay prevents the low alarm from activating whenever the doors are opened causing a brief drop in temperature 1 To mute an active high or low deviation alarm press and hold either the Up or Down arrow on the Temperature Control sub panel until the amber Mute LED illuminates and the audible alarm shuts off 2 The alarm will remain muted for the duration of the current temperature deviation tough the alarm and Mute indicators will remain illuminated 3 Any new deviation greater than 1 C will reactivate the audible alarm SET THE CO SET POINT After the unit s temperature has been calibrated and the Over Temperature Limit set enter a CO2 set point concentration level for the incubation chamber atmosphere 1 Turn on the supply to the incubator CO2 The supply should deliver 20 psi at the external inline HEPA filter O 2 Press either the Up or Down arrow button on the CO Control sub panel Set CO a The display will dim and begin to blink showing the adjustable CO set point b A flashing OFF my show if the display was set to off 3 Use the arrow buttons adjust the set point to your application s required CO concentration a Note that if the buttons are not pushed for five 5 seconds the display will revert to showing the current CO level with the last value in the display saved as the new CO set point 4 After selecting a CO set point wait five
20. bient 8 C to 60 C 0 5 37 C SCO40 Ambient 8 C to 60 C 0 5 37 C SCO31 Ambient 8 C to 60 C 0 5 37 C POWER Model AC Voltage Amperage Frequency Scoss a Pressure Units Conversion Conversion table for pressure units EE Zum com o am 5 oh ba Lan 1 mmHg XA 1 Tom 1 33322 X 1071 1 33322 X 10741 1 35951 X 10 ta 38 Page LAB PARTS AND CONSUMABLES Description Part Number D ket M oor Gasket Magnetic 3450585 SCO58 48 X 68 Inches D k 4 oor Gas si SCO 9 3450534 1 foot sections requires 20 sections D k 1 oor Gas Si SCO3 3450534 1 foot sections requires 17 25 sections Power Cord 20 Amp 2 5 Meters 1800561 Shelf Clip 1250512 Shelf Assembly SCO58 995 00014 Shelf Assembly SCO40 9750551 Shelf Assembly SCO31 995 00003 Ordering Parts and Consumables If you have the Part Number for an item you may order it directly from Sheldon Manufacturing by calling 1 800 322 4897 extension 3 If you are uncertain that you have the correct Part Number or if you need that specific item please contact Sheldon Technical Support for help at 1 800 322 4897 extension 4 or 503 640 3000 Please have the model number and serial number of the incubator ready as Tech Support will need this information to match your workstation with its correct part 39 Page LAB Sheldon Manufacturing Inc P O Box 627 Cornelius Oregon 97113 EMAIL tech Shellab com IN
21. calibration from being carried out Calibrating the CO Display 1 Connect a digital CO analyzer sample tube to the sample port on the top left side of the Incubator CO2 SAMPLE Reference Device 2 Measure the current CO2 percentage 96 inside the chamber with m the CO analyzer c ie a Compare that reading with the percentage shown on y the incubator s CO display panel b Ifthe readings are the same or fall within your laboratory Set CO protocol acceptable range the incubator is now calibrated for CO The Calibrate CO Display procedure is complete Procedure continued on next page 28 Page OPERATION CONTINUED Calibrating the CO Display Continued 3 Ifthere is a difference between the readings and that Set CO difference falls outside your laboratory protocol s acceptable range place the incubator into calibration mode by pressing and holding the CO Control Up and Down arrow buttons O a The incubator CO display will flash the letters CP and then show a blinking adjustable CO concentration value Release the arrow buttons Use the Up and Down arrows to adjust the blinking Set CO display value to match the CO analyzer reading When you have matched the display value to the reading of the CO analyzer wait for five 5 seconds Wait 5 Seconds The incubator display will cease flashing The new calibrated display Set CO value is now stored in the
22. e stabilized Wait 1 Hour Reference Device 3 Once the temperature has stabilized compare the reference device and the incubator display temperature readings If the readings are the same or or the difference between the two falls within the acceptable range of your E laboratory protocol the incubator is calibrated for temperature The Temperature Calibration procedure is complete Soe High Mute If there is a difference between the two 2 readings and that offset falls outside your laboratory protocol s acceptable range place the SCO6AD into calibration mode See the next step a Before placing the unit in calibration mode allow the temperature to re stabilize if the door has been opened This vo E Si will require a wait of at least one 1 hour Failure to do so will ch n result in an inaccurate calibration Wait 1 Hour PS v Procedure continued on next page 24 Page OPERATION CONTINUED Temperature Calibration Continued 5 Place the unit in its temperature calibration mode and calibrate a Press and hold both the UP and DOWN arrow buttons simultaneously The Temperature Display will show the letters CO then begin flashing the current temperature value Use the Up or Down arrows to adjust the current temperature value until it matches the reference device temperature reading This will correct the display s offset error If an arrow key is not pressed for five seconds the Temper
23. fter opening and closing the chamber door See page 24 for probe placement instructions Plug the power cord into the earth grounded electrical outlet Place the Power switch in the ON position Complete the following procedures a Set the Temperature Set Point page 22 b Calibrate the Temperature Display page 24 c Set the Over Temperature Limit page 27 d Set the CO2 Set Point page 27 e Calibrate the CO Display page 28 The Temperature Calibration procedure requires 24 hours to perform The CO deviation audible alarm may sound while preparing the incubator for use prior to the Set the CO2 Set Point procedure You may mute the alarm or set the CO display to Off To mute an audible alarm press and hold the Up or the Down arrow button for one 1 second on the CO control A muted alarm will stay silent for the duration of the current temperature deviation though the amber Muted indicator will remain illuminated To turn off display press either of the display arrows The display will briefly flash SP Then hold the down arrow until the display reads OFF 22 Page LAB OPERATION CONTINUED SET THE TEMPERATURE SET POINT Perform these steps to prepare the unit for calibration The unit comes from the factory set to 37 C 1 Wait 5 Seconds LAB indicated by the largest dot This prevents the Over Temperature Limit system from interfering with the Set the Temperature Set Point and Temperature
24. gs of the incubator and the reference device still fall outside your laboratory protocol after three calibration attempts contact Sheldon Technical Support for assistance a Three calibration attempts may be required to successfully calibrate units that are 2 C or more out of calibration End of Procedure SET THE OVER TEMPERATURE LIMIT The incubator temperature must be stable running at your temperature set point for at least one hour prior to setting the OTL Perform the following steps to set up the Over Temperature Limit system for normal use If you have not done so already turn the Set Over Temperature Limit control dial clockwise to the maximum position This allows the Set Temperature control to stabilize Turn the Over Temperature Limit control dial counterclockwise until the red Over Temp Limit Activated light illuminates Slowly turn the Over Temperature Limit control dial clockwise until the Over Temp Limit Activated light turns off Stop turning the control Leave the OTL control slightly above the activation point This sets the Over Temperature Limit set to approximately 1 C above the current temperature set point End of Procedure 26 Page LAB OPERATION CONTINUED MUTING THE AUDIBLE TEMPERATURE ALARM An audible and visual high or low deviation alarm will activate if the incubator chamber temperature deviates by 1 C above or below the temperature set point The low deviation alarm has a del
25. he use of three jacks or a USB style port A hardware driver and data logging software package for the USB style port can be downloaded from the Shel Lab website To download the software visit the product page of any Shel Lab CO2 incubator and click on the large USB bar icon located near the bottom of the page http www shellab com store SCO5A SHEL LAB CO2 Air Jacketed Incubator Infrared IR Sensor 5 Cu Ft 120V html The jack ports accept standard audio jacks available from most electronics retailers These are also known as 4 inch 2 pole audio connectors or phono jacks Jacks are not included with incubator Alarm activation instances are communicated directly from the main controller board through a third jack port An output is sent from the alarm port for all alarm activations Data Monitoring Systems Max Resistance For building management and other data monitoring or logging systems the maximum resistance of the current loop driven by either output from the 4 20mA module is 250 Ohms At higher loop resistances the current value will be erroneously low for parameter values near the top of the scale Output Channel Parameter Parameter Value at 4mA Parameter Value at 20mA GO 0 CO 20 CO i e ao 9 CO2 TEMP ALARM di Data Jacks and Port on the Back of the Data Jacks and Port on the Top Right SCO58 Side of the SCO40 and SCO31 33 Page LAB USER MAINTENANCE A Warning Prior to any ma
26. install four 4 clips for each shelf Two on the left wall and two on the right wall of the incubation chamber 2 Hang the shelf on the clip with the shelf front nearest the chamber door Even Shelf Spacing Intervals Front Side 14 Page LAB INSTALLATION CONTINUED CLEANING AND DEIONIZED WATER The incubator interior was cleaned at the factory but not sterilized See the Cleaning topic in the User Maintenance section for more information Never use deionized water for cleaning the incubator While DI water is useful in variety of laboratory applications it is an aggressive solvent that attacks most metals Use of DI water in a Shel Lab incubator voids the unit s warranty Sheldon Manufacturing recommends the use of distilled water in the resistance range of 50K Ohm cm to 1M Ohm cm or a conductivity range of 20 0 uS cm to 1 0 uS cm for cleaning and any humidifying applications 15 Page LAB GRAPHIC SYMBOLS The incubator is provided with multiple graphic symbols on its exterior and internal surfaces These symbols identify hazards and the functions of the adjustable components as well as important notes in the user manual Symbol Definition Indicates that you should consult your service manual for further instructions Indique que l op rateur doit consulter le manuel d utilisation pour y trouver les instructions compl mentaires Indicates Temperature Rep re temp rature Indicates the
27. intenance or service on this unit disconnect the power cord from the power supply Avertissement Avant d effectuer toute maintenance ou entretien de cet appareil d brancher le cordon secteur de la source d alimentation If a hazardous material or substance has spilled in the incubator immediately initiate your site s Hazardous Material Spill Containment protocol Contact your local Site Safety Officer and follow instructions per the site policy and procedures CLEANING AND DISINFECTING Note Do not use spray cleaners or disinfectants that might leak through openings and cracks and coat electrical components or that contain solvents that will harm coatings Do not use chlorine based bleaches or abrasives they will damage the chamber liner Warning Never clean the unit with alcohol or flammable cleaners Avertissement Ne jamais nettoyer l appareil l alcool ou avec des nettoyants inflammables The incubator chamber should be cleaned and disinfected prior to first use Periodic cleaning and disinfection are required to prevent microbiological contamination Cleaning Perform the below steps to clean the incubator 1 Remove all of the interior parts shelves racks and any additional items if assembled 2 Clean the incubator with a mild soap and water solution including all corners Do not use deionized water 3 Hinse with distilled water and wipe dry with a soft cloth 4 Take special care when cleaning around the door s
28. microprocessor controller board wired to a solid state temperature probe a heating element located in a recirculation duct as well as a blower fan in the duct to monitor and regulate the temperature within the incubation chamber SCO Incubators rely on natural heat radiation for cooling When powered and with the door closed the incubation chamber temperature cannot go below the ambient environmental temperature plus the waste heat generated by internal electrical and mechanical operations room temperature 8 C The same microprocessor board controls the concentration of CO in the incubation chamber by operating an internal gas injection solenoid valve connected to the gas input line The processor monitors CO concentration level in the incubator using an infrared sensor in the recirculation duct The sensor operates on the principle that a specific frequency set of infrared light is absorbed by CO The more CO present in the air stream from the chamber the more of that band of infrared is absorbed The sensor is only sensitive to CO so measurement accuracy is consistent regardless of the presence of other gasses in the incubator The microprocessor controller employs proportional integral derivative analytical feedback loop functions when measuring and controlling both temperature and CO levels The rate of PID controlled heating is proportional to the difference between the measured chamber air temperature and the user programed te
29. mperature set point The rate of heating slows as the air temperature nears the set point to prevent overshooting The length of gas injections is proportional to the difference between the measured concentration and the set point The frequency of injections is derived from the rate of change in the difference Integrator feedback slows the rate of injection as the concentration approaches the set point which helps prevent overshoots During normal operations heating pulses and CO injections take place in small bursts to correct for deviations of 0 1 C and 0 1 in gas concentration Heating pulses and gas injections will be frequent when first setting up the unit for use after installation and when recovering from door openings Automatic Door Cutoff Whenever the incubation chamber door is opened the SCO Incubator stops the flow of CO into the chamber depowers the heater element and ceases operation of the internal blower fan This limits the amount of CO released into the workspace around the incubator It also prevents the heater from attempting to counteract the continual inflow of cooler air which would cause a significant heat spike once the door is closed Normal CO injections heating and fan operation all resume automatically when the door is closed 20 Page LAB OPERATION CONTINUED Accessory Compatibility Make sure that any accessory equipment you will be using inside the incubator can safely and effectively oper
30. n primarily depends on either lowering the humidity level in the incubator or increasing the air temperature Intrusions of cool air into the incubator chamber or cold air blowing on the exterior of the incubator may also help cause condensation in the chamber Note Rising or falling air pressure from weather will adjust the dew point up and down in small increments If the relative humidity in the incubation chamber is already near the dew point barometric fluctuations may push it across the dew point threshold If condensation is forming in the incubation chamber check the following e ls the door closing and latching properly Is the door gasket leaking Check the gasket for damage wear or signs of brittleness or dryness Replace the gasket if needed e Are frequent or lengthy chamber door openings causing significant temperature disruptions If so reduce the number of openings e Is the incubator exposed to an external flow of cold air such as an air conditioning vent or a door to a cooler hallway or adjacent room Block or divert the air or move the incubator e Does the ambient humidity in the room exceed the incubator s stated operating range of 80 relative humidity If so lower the room s humidity e Are there are too many open containers of evaporating sample media in the chamber If so reduce the number of sample containers Remove humidity pans or other open containers of water e S CQO58 only Is the access port stop
31. nd trays and secure the door in the closed position prior to lifting the unit Do not attempt to move the unit while in operation or before the unit has cooled LEVELING The SCO Incubator must be level and stable for safe operation Each incubator ships with four leveling feet Insert one leveling foot into each of the four holes in the bottom corners of the workstation Adjust the foot at each corner until the workstation stands level and solid without rocking To raise a foot turn it in a counterclockwise direction to lower a foot turn it in a clockwise direction ACCESS PORT STOPPER The SCO58 Incubator is provided with an access port located on the right side of the unit The incubator is shipped with one 1 rubber access port stopper The stopper should come installed in the port inside the incubation chamber The stopper should always be installed in the chamber to obtain the best temperature uniformity and prevent condensation from forming inside the port A second stopper may be installed on the outside of the unit to prevent dust from building up in the port but is not required 1750570 12 Page LAB INSTALLATION CONTINUED CONNECT THE CO SUPPLY TO THE INCUBATOR Note Always use medical grade CO Use of non medical grade CO risks introducing contaminants into the chamber may damage the incubator and will void the incubator s warranty Always install the in line filter provided with the unit
32. o Gas Indicator I Alarm Alarm n9S Illuminates Activates Activates CO Alarm Activation Time Line 31 Page LAB OPERATION CONTINUED LOADING THE INCUBATOR Sheldon Manufacturing recommends allowing the unit to run for at least 24 hours heated to temperature and supplied with CO prior to loading samples after installing in a new location Place items on the shelves inside the incubator chamber as evenly spaced as possible Good spacing allows for maximum air circulation and a high degree of temperature uniformity ATTACHING EQUIPMENT TO THE INTERIOR ACCESSORY OUTLETS The SOC Incubators are provided with accessory power outlets located inside the chamber The power switch on the control panel controls power to these outlets Equipment attached to the outlets should not exceed 1 Amp in draw Accessory equipment may produce heat This heat could affect the temperature stability and uniformity of the incubator Check that the incubator operates at temperature when the accessory equipment is installed and operating Note Do not attach any equipment drawing more than 1 amp to an outlet 32 Page LAB OPERATION CONTINUED DATA OUTPUT CAPABILITIES The SCO Incubators generate data outputs describing temperature and CO percentage levels using a 4 through 20 milliamp bus analog output module These outputs can be connected to a building management system BMS or other data monitoring and capture system through t
33. per in place and properly seated Reinsert the stopper or replace if damaged or worn 36 Page LAB UNIT SPECIFICATIONS The SCO Incubator is a 115 voltage unit Please refer to the incubator s data plate for individual electrical specifications Technical data specified applies to units with standard equipment at an ambient temperature of 25 C and a voltage fluctuation of 10 The temperatures specified are determined in accordance to factory standard following DIN 12880 respecting the recommended wall clearances of 10 of the height width and depth of the inner chamber All indications are average values typical for units produced in the series We reserve the right to alter technical specifications at all times Model Shipping Net Weight SCO58 850 Ibs 385 55 kg 710 Ibs 322 kg SC040 850 Ibs 385 55 kg 710 Ibs 322 kg SCO31 660 Ibs 299 37 kg 610 Ibs 276 69 kg Exterior W x DxH Interior W x DxH 50 5 x 44 5 x 86 inches 43 x 35 x 68 inches 42 5 x 34 5 x 90 inches 35 x 26 x 75 5 inches 39 5 x 33 8 x 75 3 inches 32 7 x 26 x 63 inches Exterior W x DxH Interior W x Dx H 128 x 113 x 218 cm 109 22 x 88 9 x 172 72 cm 108 x 87 7 x 228 6 cm 88 9 x 66 x 191 7 cm 100 4 x 85 8 x 191 2 cm 83 1 x 66 x 160 cm Cubic Feet Cubic Liters UNIT SPECIFICATIONS CONTINUED CO2 Model Range All Models 0 20 Concentration TEMPERATURE Model Range Uniformity Stability SCO58 Am
34. rict safety requirements Only use this equipment for its intended application Any alterations or modifications void the warranty For continued safe operation of your incubator always follow basic safety precautions including 4 Page Follow all local ordinances in your area regarding the use of this incubator If you have any questions about local requirements please contact the appropriate local agencies The power supply for the unit must be an earth grounded electrical outlet that conforms to national and local electrical codes If the incubator is not grounded properly parts such as knobs and controls can conduct electricity and cause serious injury Always use the power cord supplied with the unit or an identical replacement cord Avoid damaging the power cord Do not bend it excessively step on it or place heavy objects on it A damaged cord can be a shock or fire hazard Never use a power cord if it is damaged Position the unit so that the user can quickly unplug the cord in the event of an emergency Use only approved accessories LAB INTRODUCTION CONTINUED ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its products As a result engineering changes and improvements are made from time to time Therefore some changes modifications and improvements may not be covered in this manual If your unit s operating characteristics or appearance differs from those described in this manual
35. the incubator If the carton is not damaged open the carton and remove the contents The unit should come with an Installation and Operation Manual warranty card and a Certificate of Compliance Verify that the correct number of the following accessories are present Included Accessories CO Tubing Kit Shelves Shelf Clips 115V Power Cord SCO58 1 6 36 1 SCO40 1 6 24 1 SCO31 1 6 24 1 Carefully check all packaging before discarding Save the shipping carton until you are sure everything works properly 6 Page LAB RECEIVING YOUR INCUBATOR CONTINUED Orientation Photos SCO58 Control Panel CO Inlet left CO Sample Port right Data Ports N Power Inlet Na Circuit Breaker Access Port OTL Temperature Probe Top Controller Board Temperature Probe Bottom Incubation Chamber Back Wall Shelf Mounting Standard Door Gasket Incubator Door 7 Page RECEIVING YOUR INCUBATOR CONTINUED SCO40 Control Panel CO Inlet CO Sample Port Data Jacks USB Style Port OTL Temperature Probe Top Main Temperature Probe Bottom Incubation Chamber Power Inlet Circuit Breaker and Data Plate Door Gasket Incubator Door 8 Page RECEIVING YOUR INCUBATOR CONTINUED SCO31 Control Panel CO Inlet CO Sample Port Data Jacks USB Style Port OTL Temperature Probe Top Controller Board Temperature Probe
36. unit The display will revert to showing the i current CO level in the chamber and the incubator will adjust the chamber CO level to match the current set point 5 Allow the incubator sit for one 1 hour undisturbed to Set CO stabilize after it has achieved the CO set point with the corrected display value Failure to wait until the unit is fully stabilized will result in an inaccurate reading during the next Wait 1 Hour step A Procedure continued on next page 29 Page LAB OPERATION CONTINUED Calibrating the CO Display Continued Reference 6 Compare the reference CO analyzer reading to the reading of the incubator CO display a If the reference device and the incubator display Set CO readings are the same or the difference falls within the range of your laboratory protocol the incubator is now calibrated for CO If the readings falls outside your laboratory protocol s range enter a new adjustment offset repeat steps 3 6 starting on page 29 Set CO a Repeat for a total of three attempts If the gas CO concentration readings of the incubator and the Note Three attempts reference device still fall outside your laboratory protocol after three may be required to calibration attempts contact Sheldon Technical Support for sucessfully calibrate a assistance unit that is more than 2 out of calibration End of procedure 30 Page LAB OPERATION
37. witch temperature sensor heads and chamber power outlets to prevent damage Procedure continued on next page 34 Page LAB MAINTENANCE CONTINUED Disinfecting Disinfect the incubator on a regular basis Perform the following steps to disinfect the workstation 1 Turn the unit off Carryout your laboratory clinical or production space disinfection protocol 2 If possible remove all interior accessories shelving and other non attached items from the chamber when disinfecting Disinfect all corners the incubation chamber Take special care when disinfecting around the temperature probes 3 Disinfect the incubator using commercially available disinfectants that are non corrosive non abrasive and suitable for use on stainless steel surfaces Contact your local Site Safety Officer for detailed information on the disinfectants compatible with your cultivation or culturing applications 4 Do not use overtly volatile disinfecting agents Chlorines amphyls and quaternary ammonias will evaporate into the chamber environment Over time the concentration in the chamber atmosphere will continue to increase potentially leading to inhibited growth or metabolic symptoms in sample populations End of procedure MAINTAINING ATMOSPHERIC INTEGRITY Periodically inspect the door latch trim catch and gasket for signs of deterioration Failure to maintain the integrity of the door system shortens the life span of the incubator
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