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Agilent 1200 Series Variable Wavelength Detector

1. Optical Unit 1200 Series Variable Wavelength Detector Service M anual Source Lens and Filter Assembly Table 30 Source Lens and Filter Assembly Parts for Repairs 14 Item Description Part Number 1 Lens assembly complete G1314 65008 2 Filter assembly top G1314 65010 I f x Pi 2 Figure 63 Source Lens and Filter Assembly 1200 Series Variable Wavelength Detector Service M anual 245 14 Parts for Repairs Grating Assembly Do not disassemble the Grating M otor Assembly Its height is factory aligned Table 31 Grating Assembly Item Description Part Number 1 Screw Grating M3 5 mm lg see 2 Grating Assembly includes Test Slit and Seal for Optical Cover G1314 65007 3 Spacer see 4 Position Sensor includes Test Slit and Seal for G1314 65005 Optical Cover 5 Screw Sensor M3 6 mm lg see 6 Grating M otor Assembly excluding heat sink parts G1314 65009 includes Test Slit and Seal for Optical Cover 7 Screws M4 10 mm lg see 8 Sealing G1314 80012 9 Heat Sink G1314 40002 10 Screws M3 6mmlg see 11 Cover part of bottom plate of optical unit no part number Miscellaneous Kit includes screws washers and spacers used for Grating and Filter Assembly G1314 65022 246 1200 Series Variable Wavelength Detector Service M anual Parts for Repairs 14 11 Figure 64 Grating A
2. Connector Pin Pin Signal Name 01040 60101 3390 2 3 Agilent 1200 1 Shield Ground 2 Not connected B 3 Center Signal eag 4 Connected to pin 6 kE BRN 5 Shield Analog i BRN 6 Connected to pin 4 7 Key 8 Not connected 1200 Series Variable Wavelength Detector Service M anual Identifying Cables 15 Agilent 1200 to 3394 6 Integrators Connector Pin Pin Signal Name 35900 60750 3394 6 Agilent 1200 1 Not connected 2 Shield Analog 3 Center Analog GINO Agilent 1200 to BNC Connector Connector Pin Pin Signal Name 8120 1840 BNC Agilent 1200 Shield Shield Analog Center Center Analog 1200 Series Variable Wavelength Detector Service M anual 261 15 Identifying Cables Agilent 1200 to General Purpose Connector Pin Pin Signal Name 01046 60105 3394 6 Agilent 1200 1 Not connected 2 Black Analog 3 Red Analog p ae 262 1200 Series Variable Wavelength Detector Service M anual Remote Cables Identifying Cables 15 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1200 Series modules The other end depends on the instrument to be connected to Agilent 1200 to 3390 Integrators Connector Pin Pin SignalName Active 01046 60203 3390 Agilent 1200 TTL 2 1 White Digita
3. Item Description Part Number 1 Front cover 5065 9982 2 Plastics includes base sides and top 5065 9985 3 Name plate Agilent 1200 Series 5042 8901 For correct assembling of the top and sides see Assembling the Main Cover on page 227 Figure 68 Plastic Parts 1200 Series Variable Wavelength Detector Service M anual Parts for Repairs 14 Leak Parts Table 36 Leak Parts Item Description Part Number 1 Leak sensor assembly 5061 3356 2 Leak pan 5042 8908 3 Leak funnel 5041 8388 4 Leak funnel holder 5041 8389 5 Clip 5041 8387 6 Corrugated tubing 120 mm lg re order 5 m 5062 2463 7 Corrugated tubing 1200 mm lg re order 5 m 5062 2463 Figure 69 Leak Parts 1200 Series Variable Wavelength Detector Service M anual 253 14 Parts for Repairs Foam Parts 254 Table 37 Foam Parts Item Description Part Number 1 2 EPP foam kit includes G1314 65019 Base and top part 1 and 2 3 Damper kit includes bumpers for optical and fan G1314 65021 4 Guides for interface board 5041 8395 Do not order the individual part numbers mentioned on the foam Figure 70 Foam Parts 1200 Series Variable Wavelength Detector Service M anual Parts for Repairs 14 Power and Status Light Pipes Table 38 Power and Status Light Pipes Item Description Part Number Power supply assembly 0950 2528 Screw M4 x 0 7 8 mm lg to fix power supply at rear panel 0515 0910 Washer 2190 0409 1 Power ligh
4. 3 J 201 J 207 j 209 J 205 v J 400 ADC board Sample J 203 J 401 ADC Board Reference gt as 1 J 204 J 502 Deuterium lamp T seseggezss es S1 Configuration switch J 400 J401 Figure 48 Location of Connectors on VWM Board 7 Remove the processor board Place the board on the ESD kit If you also have to replace other assemblies continue with that work first 8 On the new board check the switch setting of address switch S1 see Setting the 8 bit Configuration Switch on page 295 1200 Series Variable Wavelength Detector Service M anual 167 12 Repair An incorrect switch setting for example TEST BOOT may cause the module to turn in a basic mode yellow or red flashing status light In such a case turn off the module re set the address switches and turn on the module again 9 Install the new processor board and reconnect the connectors Make sure that the board is fitted correctly in the board recesses in the holes at the rear panel 10 Refit the screws at the REMOTE and GPIB connectors and the nut of the analog connector 11 Reinstall the top foam section top cover and front cover see Installing the Foam and the Top Cover on page 225 12 Replace detector into the stack and reconnect the cables If anew VWM board was installed update the serial number information of the detector in the user interface see
5. E T Waste outlet eak sensor assembly Figure 43 Replacing Waste Handling System Parts 1200 Series Variable Wavelength Detector Service M anual 149 11 Maintenance Replacing the Interface Board When required detector Part required When defective or for installation of the board or for all repairs inside the Interface board BCD G1351 68701 with external contacts and BCD outputs see BCD Board on page 287 LAN Communication Interface board G1369A or G1369 60001 see LAN Communication Interface Board on page 289 Tools required None 1 Install the ESD strap M ove the power lock across the power inlet ESD strap 2 If required unscrew and remove the interface board Place the board on the ESD kit 3 If required insert the interface board and fix the screws Next steps Remove the ESD strap Reinstall the module into the stack 150 1200 Series Variable W avelength Detector Service M anual Maintenance 11 Replacing the Detector s Firmware When required If new version solves problems of currently installed version or after exchange of the detector main board VW M the version on board is older than previous installed one Tools required LAN RS 232 Firmware Update Tool or Instant Pilot G4208A or Control Module G1323B Parts required Firmware tools and documentation from Agilent web site Preparations Read update documentation provided with the Fi
6. Using the Control M odule G1323B Connect the control module to the detector Turn ON the detector On the control module press System F5 then Records F4 Using the up down arrows make sure that the detector is highlighted Press FW Update F5 then m This will display a box which says Update Enter Serial Press Enter This will display the box labeled Serial 1200 Series Variable Wavelength Detector Service M anual 171 12 Repair Letters and numbers are created using the up and down arrows Into the box labeled Serial enter the 10 character serial number for the detector When the 10 character serial number is entered press Enter to highlight the complete serial number Then press Done F6 Turn the detector OFF then ON again The Records screen should display the correct serial number for this module If a Agilent ChemStation is also connected restart the Agilent ChemStation now as well To change the product number go to the System screen 1 2 3 4 Press Tests F3 and select the detector and press Enter While in the Tests screen press m m m dot m From the box now displayed select the Command and press Enter Into the box labeled Nester instruction enter the command TYPE XXXXX Letters and numbers are created using the up and down arrows XXXXX is the 5 character product number of the module being changed There must be a space between the word TYPE and the product number Examp
7. 1200 Series Variable Wavelength Detector Service M anual 179 12 Repair 2 Locate the source lens and filter assembly 3 Using a tweezers counter hold the filter assembly Unscrew and remove the top part of the filter assembly Source lens Filter assembly ce assembly ae TOT Ky iM ian a G JT 8 yhe __ Y 2 ee m o oo fe ieee ice Il Al mir APS IL 4 Loosen the setscrew and slide the lens assembly out of Note mSNOleh Unscrew the insert and place the assembly as shown The lens cannot be removed It is cleanable with alcohol from the outside Do not scratch the lens nor get contaminations onto it 180 1200 Series Variable Wavelength Detector Service M anual Repair 12 5 Slide the lens assembly into its position Center the marker on the lens assembly Fix it the setscrew 6 Observe the orientation of the filter assembly top part Install the assembly and fix the screws Next steps Replace the bumpers in the top foam so that they are positioned on the optical unit Reinstall the front cover top cover and top foam section see Installing the Foam and the Top Cover on page 225 Replace the front cover Replace the detector into the
8. Install the assembly and fix the screws 1200 Series Variable Wavelength Detector Service M anual 183 12 Repair 5 Replace the cover of the optical unit Next steps Replace the bumpers in the top foam so that they are positioned on the optical unit Reinstall the front cover top cover and top foam section see Installing the Foam and the Top Cover on page 225 Replace the front cover Replace the detector into the stack Reconnect the cables and capillaries and turn ON the detector 184 1200 Series Variable Wavelength Detector Service M anual Repair 12 Installing the Test Slit When required This procedure has to be carried out at the beginning of all replacement procedures Tools required Screwdriver POZI 1 PT3 Wrench 1 4 1 5 inch A pointed pair of pliers Parts required Test Slit part of Slit Kit 79853 68746 Preparation for this procedure are 1 Remove the top cover of the optical unit Take care for the reference aperture handle Turn OFF the module Disconnect the power cable Disconnect capillaries Remove module from stack and place it on the working bench Remove the front cover top cover and top foam section front part 1200 Series Variable Wavelength Detector Service M anual 185 12 Repair 3 Remove the standard slit from the entrance slit holder using a pair of tweezers and place it safe 2 Locat
9. Leak Sensor Open 88 Leak Sensor Short 89 Compensation Sensor Open 90 Compensation Sensor Short 91 Fan Failed 92 Open Cover 93 Detector Error Messages 94 Lamp Current Missing 94 Lamp Voltage Missing 95 Lamp Ignition Failed 96 Heater Current Missing 97 Calibration Failed 98 Holmium Oxide Test Failed 99 Grating Filter M otor Test Failed 100 Wavelength Check Failed 101 Filter Check Failed 102 8 Test Functions Available Tests versus Interfaces 104 Wavelength Verification Calibration 105 When to Calibrate the Detector 106 Zero Order Calibration 106 656 nm Wavelength Calibration 106 Lamp on Routine 107 Checking the Photocurrent 108 Holmium Oxide Test 109 Intensity Test 112 Dark Current Test 114 DAC Test 116 Grating Motor Test 117 1200 Series Variable Wavelength Detector Service M anual Filter M otor Test 118 Test Chromatogram 119 9 Diagnosis Signals Diagnostic Signal Output 122 Service Dialog 124 Signal Descriptions 126 10 Maintenance and Repair 11 Maintenance Introduction to M aintenance and Repair 130 Simple Repairs Maintenance 130 Exchanging Internal Parts Repair 130 Warnings and Cautions 131 Cleaning the Detector 132 Using the ESD Strap 133 Overview of Maintenance 136 ExchangingaLamp 137 Exchanging a Flow Cell 139 Repairing the Flow Cells 142 Using the Cuvette Holder 145 Correcting Leaks 148 Replacing Leak Handling System Parts 149 Replacing the Interface Board 150 Replacing the D
10. Repairs in the Bottom Section Following parts can be replaced within the bottom section of the optical unit e Filter Motor Drive parts e Grating Drive parts e Pre amplifier boards Grating Drive Assembly Filter Drive Assembly I o Figure 53 Location of Replaceable Parts in the Optical Unit Bottom Section 1200 Series Variable Wavelength Detector Service M anual 201 12 Repair Removing the Optical Unit When required When other assemblies have to be removed or when defective Tools required Screwdriver POZI 1 PT3 Parts required Optical unit G1314 69060 exchange part Preparation for this procedure are Turn OFF the detector Disconnect the power cable Disconnect capillaries Remove the detector from stack and place it on the working bench see Removing the Top Cover and Top Foams on page 163 Remove the front cover top cover and top foam section 1 Keep the bumpers safe 202 1200 Series Variable Wavelength Detector Service M anual Repair 12 2 Remove the lamp cover and the plate above the flow cell 3 Disconnect the deuterium lamp connector and all cables coming from the optical unit to the main board 1200 Series Variable Wavelength Detector Service M anual 203 12 Repair Exchanging a Photo Diode and or ADC Board Assembly When required The sample or reference diode ADC board assembly might be replaced when e
11. e introcduction and specifications e installation e using and optimizing e troubleshooting and diagnose e maintenance and repair e parts identification e hardware information e safety and related information Agilent Technologies 2006 Printed in Germany Edition 02 2006 GH tA 80110 G1314 90110 EE Agilent Technologies
12. Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting 1200 Series Variable Wavelength Detector Service M anual 305 A Appendix Safety Symbols Table 58 shows safety symbols used on the instrument and in the manuals Table 58 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to AN protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal A w Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product amp WARNING A warning alerts you to situations that could cause physical injury or damage to the equipment Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution alerts you to situations that could cause a possible loss of data Do not proceed beyond a caution until you have fully understood and met the indicated conditions 306 1200 Series Variable Wavelength Detector Service M anual Appendix A Lithium Batteries Information Danger of explosion if battery is incorrectly replaced Replace only with the same or equivalent type recommended by the equipment manufacturer Lithium batteries may not be disposed off into the domestic waste Transportation of d
13. V Check the degasser module for an error condition Refer to the Reference Manual for the Agilent 1200 Series vacuum degasser 84 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Remote Timeout A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable Causes e Not ready condition in one of the instruments connected to the remote line e Defective remote cable e Defective components in the instrument showing the not ready condition Suggested Actions V Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis V Exchange the remote cable V Check the instrument for defects refer to the instrument s reference documentation 1200 Series Variable Wavelength Detector Service Manual 85 7 86 Error Information Synchronization Lost During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system
14. a 3 a 5 Figure 11 Turning on the HPLC Module 50 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 5 Purge the pump For more information see Priming and Purging the System on page 44 6 Allow the detector to warm up of at least 60 minutes to provide a stable baseline see example in Figure 12 For reproducible chromatography the detector and lamp should be on for at least one hour Otherwise the detector baseline may still drift depending on the environment VWI A Wavelangtha2S4 am LAMPONIVA_LAMPON D man o2 a Bia a a ee ee Stee IOS 9 kad x s o min Figure 12 Stabilization of Baseline 1200 Series Variable Wavelength Detector Service M anual 51 4 Using the Detector 7 For the isocratic pump fill the solvent bottle with the mixture of HPLC grade bi distilled water 80 and acetonitrile 70 For binary and quaternary pumps you can use separate bottles 8 Click on the Load Method button and select DEF_LC M and press OK Alternative double click on the method in the method window The default LC method parameters are transferred into the 1200 modules Load Method System z 22 None Fe D DEF LEM DEMUCALI M DEMOCAL M OGALSTST M OGCALAS M OGCALOQ1M OGCALOQ2M Method Sequence View Abert Hep sammes OB h cerae Q enoc QI oasasrstm occas W oxmogm 5 Y caog U oxncgsm caog W Onn
15. 1200 Series Variable Wavelength Detector Service M anual Troubleshooting and Diagnostics 6 User Interfaces Depending on the user interface the available tests vary All test descriptions are based on the Agilent ChemStation as user interface Some descriptions are only available in the Service Manual Table 13 Test Functions available vs User Interface Test ChemStation Instant Pilot Control M odule G4208A G1323B Selftest Yes No No Filter Yes No No Slit Yes No Yes D A Converter Yes No No Test Chromatogram Yes C No Yes Wavelength Calibration Yes Yes M Yes Lamp Intensity Yes Yes D Yes Holmium Yes Yes D Yes Cell Yes Yes D No Dark Current Yes Yes D No C via command M section M aintenance D section Diagnose The Agilent Control M odule G1323B does not do any calculations So there will be no reports generated with passed failed information 1200 Series Variable Wavelength Detector Service M anual 79 6 Troubleshooting and Diagnostics Agilent LC Diagnostic Software 80 The Agilent LC diagnostic software is an application independent tool that provides troubleshooting capabilities for the Agilent 1200 Series modules It provides for all 1200 Series LC the possibility of a first guided diagnostic for typical HPLC symptoms and a status report stored as Adobe Acrobat pdf or as a printable file to assist users evaluating the instrument state At the introduction following modules will be
16. 190 to 600 nm e optional flow cell cartridges standard 10 mm 14 ul high pressure 10 mm 14 ul micro 5 mm 1 ul semi micro 6 mm 5 ul are available and can be used depending on the application needs e easy front access to lamp and flow cell for fast replacement and e built in holmium oxide filter for fast wavelength accuracy verification For specifications Performance Specifications on page 29 Two version of the Agilent 1200 Series variable wavelength detector are available G1314B VWD 1200 Series Variable Wavelength Detector standard version G1314C VWD SL 1200 Series Variable Wavelength Detector SL high data rates for fast HPLC The G1314C VW D SL can be operated with a G1323B Control Module just in standard mode as G1314B no higher data rate selection is available 14 1200 Series Variable Wavelength Detector Service M anual Introduction to the Variable Wavelength Detector 1 Optical System Overview The optical system of the detector is shown in Figure 1 Its radiation source is a deuterium arc discharge lamp for the ultraviolet UV wavelength range from 190 to 600 nm The light beam from the deuterium lamp passes through a lens a filter assembly in position none cut off or holmium oxide an entrance slit a spherical mirror M1 a grating a second spherical mirror M2 a beam splitter and finally through a flow cell to the sample diode The beam through the flow cell is absorbed depending on the solutions in
17. 8 data bit with no parity Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnose and repair log books which will not be erased If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 55 Forced Cold Start Settings Mode Select 1 2 3 4 5 6 7 8 TEST BOOT 1 1 0 0 0 0 0 1 To return to normal operation set switches back to your GPIB or RS 232 configuration settings 1200 Series Variable Wavelength Detector Service M anual Stay Resident Settings Hardware Information 16 Firmware update procedures may require this mode in case of firmware loading errors Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it is not operable as a detector It only uses basic functions of the operating system for example for communication Table 56 Stay Resident Settings Mode Select 1 2 TEST
18. BOOT 1 1 To return to normal operation set switches back to your GPIB or RS 232C configuration settings 1200 Series Variable Wavelength Detector Service M anual 299 16 Hardware Information Main Power Supply Assembly The main power supply comprises a closed assembly no on site repair possibility The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting MPS 36V 36V not used supply line 24V gt primary o voltage circuits 424V 45A 5V switching gt 45V 3A regulator linear voltage gt V A regulators gt 15V 3A Figure 80 Main Power Supply MPS Blockdiagram WARNING To disconnect the instrument from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned OFF 300 1200 Series Variable W avelength Detector Service M anual Hardware Information 16 No accessible hardware fuse is needed because the main power supply is safe against any short circuits or overload conditions on the output lines When overload conditions occur the power supply turns off all output voltages Turning the line power off and on again resets the power su
19. General Safety Information 304 The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards This instrument is designed and certified as a general purpose laboratory instrument for research and routine application only It is not certified for in vitro or medical applications Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure
20. No RS 232C Yes Yes Yes Yes Yes Yes No Remote Yes Yes Yes Yes Yes Yes Yes Analog Yes No 2x 2 x 1x No Yes Interface board Yes Yes Yes No Yes No No LAN BCD Ext The vacuum degasser will have a special connector for specific use For details see description of main board 290 e CAN connectors as interface to other Agilent 1200 Series modules e GPIB connector as interface to the Agilent ChemStation e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e analog output connector s for signal output and e interface slot for specific interfacing external contacts BCD LAN and so on For identification and location of the connectors see Figure 8 on page 38 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations see on page 238 1200 Series Variable W avelength Detector Service M anual Analog Signal Output Hardware Information 16 The analog signal output can be distributed to a recording device For details refer to the description of the main board of the module GPIB Interface The GPIB connector is used to connect the module with a computer The address and control switches next to the GPIB connector determine the GPIB address of your module The switches are preset to a default address and recognized by the operating software from Agilent Technologies Table
21. Place the detector in the stack or on the bench in a horizontal position 3 Ensure the line power switch at the front of the detector is OFF Status indicator green yellow red Line power switch with green light Figure7 Front View of Detector The figure above shows the flow cell already installed The flow cell area is closed with a metal cover The flow cell has to be installed as described in Flow Connections to the Detector on page 40 1200 Series Variable Wavelength Detector Service M anual 37 3 Installing the Detector 4 Connect the power cable to the power connector at the rear of the detector 5 Connect the CAN cable to other Agilent 1200 Series modules 6 Ifa Agilent ChemStation is the controller connect either the LAN connection to the LAN interface board in the detector If a Agilent 1200 DAD M W D FLD is in the system the LAN should be connected to the DAD MWD FLD due to higher data load 7 Connect the analog cable optional 8 Connect the APG remote cable optional for non Agilent 1200 Series instruments 9 Turn ON power by pushing the button at the lower left hand side of the detector The status LED should be green Security lever Interface board Analog signals APG remote SS o lf RS 232C CAN GPIB Power Configuration switch Figure8 Rear View of Detector 38 1200 Series Variable Wavelength Detector Service M anual Installing the Detector 3 The detecto
22. Variable Wavelength Detector Service M anual Identifying Cables 15 Agilent 1200 to HP 1090 LC or Signal Distribution M odule Connector Pin Pin SignalName Active 01046 60202 HP 1090 Agilent 1200 TTL 1 1 White Digital ground NC 2 Brown Prepare run Low 2 8 4 3 Gray Start Low 7 LA 6 7 4 Blue Shut down Low S 4 45 8 5 Pink Not connected a i NC 6 Yellow Power on High 3 7 Red Ready High 5 Key 6 8 Green Stop Low NC 9 Black Start request Low Agilent 1200 to General Purpose Connector Pin Pin SignalName Active 01046 60201 Universal Agilent 1200 TTL 1 White Digital ground o 2 Brown Prepare run Low A il zE 3 Gray Start Low OO BE a 4 Blue Shut down Low lt oo m 5 Pink Not connected o0 D5 6 Yellow Poweron High oO Sloj 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 1200 Series Variable Wavelength Detector Service M anual 267 15 Identifying Cables BCD Cables One end of these cables provides a 15 pin BCD connector to be connected to the Agilent 1200 Series modules The other end depends on the instrument to be connected to Agilent 1200 to General Purpose Connector Wire Color Pin SignalName BCD Digit G1351 81600 Agilent 1200 Green 1 BCD 5 20 Violet 2 BCD 7 80 I Blue 3 BCD 6 40 EZ Yellow 4 BCD 4 10 Eo o Black 5 BCD 0 1 SS Orange 6 BCD 3 8 Red 7 BCD 2
23. and other parameters C isthe concentration of the absorbing species usually in g or mg l and d is the path length of the cell used for the measurement Therefore flow cells with longer path lengths yield higher signals Although noise usually increases little with increasing path length there is a gain in signal to noise ratio For example in Figure 27 on page 72 the noise increased by less than 10 but a 70 increase in signal intensity was achieved by increasing the path length from 6 mm to 10 mm When increasing the path length the cell volume usually increases in our example from 5 13 ul Typically this causes more peak dispersion As Figure 27 on page 72 demonstrates this did not affect the resolution in the gradient separation in our example As a rule of thumb the flow cell volume should be about 1 3 of the peak volume at half height To determine the volume of your peaks take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3 1200 Series Variable Wavelength Detector Service M anual 71 5 How to optimize the detector Analysis of pesticide standard 6 mm optical path length 10 mm optical path length Time min Figure 27 Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards To check photometric accuracy of the Agilent 1200 S
24. and stop time according to Table 15 2 Select the function Enable test chromatogram in Tests VWD and press Execute 3 Select Plot in the System screen and press Setup 4 Select as Source the Signal VWD change the Y range to 10 to 300 mAU fit the Time Range to match Table 15 and press Done 1200 Series Variable Wavelength Detector Service M anual 119 8 Test Functions 120 5 If required connect a recording device for example Agilent 3396A with settings ATTN 9 CHSP 2 ZERO 10 AR REJ 106 6 Press Start to start the run On completion of the run or when the run is stopped the test chromatogram is deactivated ADC1 A Analog Output TEST TESTCR2S D mV 350 4 300 4 250 4 200 4 gt 1501 150 2 g 8 g S EOY ka 100 4 N N SI fp F Nay oe AON 50 4 Figure 35 Test Chromatogram on Integrator response time 2 seconds Agilent ChemStation 1 Select the response time and stop time according to Table 15 on page 119 2 Activate the test chromatogram by typing into the command line or as pre run command PRINT SENDMODULE LVWD SIMU DFLT 3 Start the run On completion of the run or when the run is stopped the test chromatogram is deactivate VWD1 A Wavelength 254 nm TEST TESTCR2S D 250 4 200 4 150 4 100 4 1478 J 2 846 T T 15 2 25 3 min Figure 36 Test Chromatogram on Agilent ChemStation re
25. and the Online Spectra window see Online Spectra on page 60 displays the result Sample Scan minus Blank Scan 62 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 Analog Output Settings 1 To change the Output Range of the analog outputs select VWD Control 2 To change the offset and the attenuation select VWD Signal More E Set up YO Ser QE Set up ignal WD Control System 2 x 6 Analog Output Range can be of Control plam Error Method set to either 100 mV or 1 V full Eu Online Spectra J 2a ion J Take current method scale Not Ready Information P k RARES S C off e Zero Offset can be set to either pp Analog Output Range 100 mV or 1 V full scale SM DIY Attenuation Limits 0 98 to 4000 mAU at discrete values for either 100 mV or 1 V full scale At Power On m Lamp Type T Tum lamp on a131 4 60100 m Automatic Turn On I Tum lamp on at lt dd rim ypyp gt lt hhemmiss gt OK Cancel Help of Control m Signal Time gt Analog Output fy Online Spectra r wavelength Stoptime Zero Offset Not Ready Information 254 as Pump SS mi 5 x Help mn no Limit 2 M 5 Peakwidth Responsetime Posttime Attenuation gt 01min 2s ot f min 1000 7 mau Figure 23 Analog Output Settings 3 Change the values if required 1200 Series Variable Wavelength De
26. aos DEFAULT O W oxurs u W oscerrst M W PSIM OFTST W carcut m owe W osetest m Q ootreetst m W awit m E data Yoon 51 168 nee Yoron pa og z 33 z338 Figure 13 Loading Default LC M ethod 52 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 9 Click on the module icons Figure 14 and open the Setup of these modules Figure 15 on page 54 shows the detector settings do not change the detector parameters at this time OEFAULT D gt TSet up VWD Signal of Control fy Online Spectra Q Data Curves of Control 8 Not Ready Information B Not Ready Information Help Help Figure 14 Open the module menu 10 Enter the pump parameters mentioned under Conditions on page 47 1200 Series Variable Wavelength Detector Service Manual 53 4 Using the Detector xl m Signal r Time Wavelength Stoptime 254 nm as Pump min no Limit Peakwidth Responsetime Posttime gt O1min 2s Off 4 min m Timetable Table Graphic i x r Analog Output Cancel Help Zero Offset 5 E Attenuation 1000 mAU r Store additionally T Signal w o Reference J Reference only m Autobalance IV Prerun J Postrun m Special Setpoints Setup 1 signal with individual wavelength setting stop and post time can be set if
27. beta emission line at 486 nm and the three holmium lines are used for the complete wavelength calibration process These holmium lines are at 360 8 nm 418 5 nm and 536 4 nm WD Wavelength Calibration System 2 xj i Deviation MMEREEPEEEEE 12nm Zero order 12nm Max Deviation UUNEEE per nt 12nm Reference lines 12nm Calibration history Deviation Zero order Ref lines Time Date 8 8nm 0 8mm 21 55 49 07 02 1970 0 0nm O1nm 11 40 47 04 01 2006 Zero order deviation is 0 0nm Max deviation reference lines 0 0nm Adjust OK Cancel Calibration settings equal to measured one no calibration necessary Figure 29 Wavelength Verification Calibration The wavelength verification calibration takes about 2 5 minutes and is disabled within the first 10 minutes after ignition of the lamp because initial drift may distort the measurement When the lamp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically see Lamp on Routine on page 107 1200 Series Variable Wavelength Detector Service M anual 105 8 106 Test Functions When to Calibrate the Detector The detector is calibrated at the factory and under normal operating conditions should not require recalibration However it is advisable to recalibrate e after maintenance flow cell or lamp e after repair of components in the optical unit e after exchange of the optical unit or VWM board e ata regu
28. converter 5061 3378 1090 liquid chromatographs 01046 60202 Signal distribution module 01046 60202 258 1200 Series Variable Wavelength Detector Service M anual Table 39 Cab les Overview continued Identifying Cables 15 Type Description Part Number BCD 3396 integrator 03396 60560 cables General purpose spade Lugs G1351 81600 Auxiliary Agilent 1200 Series vacuum degasser G1322 61600 CAN Agilent 1200 module to module 0 5 m 5181 1516 cables Agilent 1200 module to module 1 m 5181 1519 Agilent 1200 module to control module 61323 81600 External Agilent 1200 Series interface board to general purpose G1103 61611 contacts GPIB Agilent 1200 module to Agilent ChemStation 1 m 10833A cable Agilent 1200 module to Agilent ChemStation 2 m 10833B RS 232 Agilent 1200 module to a computer 34398A cable This kit contains a 9 pin female to 9 pin female Null M odem printer cable and one adapter LAN cable Cross over network cable shielded 3 m long 5023 0203 for point to point connection Twisted pair network cable shielded 7 m long 5023 0202 for hub connections 1200 Series Variable Wavelength Detector Service M anual 259 15 260 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent 1200 Series modules The other end depends on the instrument to which connection is being made Agilent 1200 to 3390 2 3 Integrators
29. detector and disconnect the cables Remove the detector from the stack and place it on the working bench PrP WN Pe Remove the front cover top cover and top foam section see Removing the Top Cover and Top Foams on page 163 Remove the processor board see Exchanging the Main Board on page 166 vi Remove the fan assembly see Exchanging the Fan on page 173 7 Remove the optical unit see Removing the Optical Unit on page 202 218 1200 Series Variable Wavelength Detector Service M anual Repair 12 8 Carefully remove the bottom foam part by sliding it out 9 Unscrew the power supply at the rear of the detector towards the rear 000000 10 Press down the power switch light pipe to remove it from 11 Remove the power supply completely Reuse the coupler the coupler on the new power supply Power switch light pipe NOTE The repair level of the power supply assembly is exchanging of the complete assembly No serviceable parts are inside 1200 Series Variable Wavelength Detector Service M anual 219 12 Repair 12 Insert the power supply into its location and fix it with the screw at the rear panel 13 Press down and clip in the power switch light pipe into the power supply Power switch light pipe drain 15 Route the lamp cable and the leak sensor cable
30. foam 1200 Series Variable Wavelength Detector Service Manual 93 7 Error Information Detector Error Messages These errors are detector specific Lamp Current Missing The lamp anode current is missing The processor continually monitors the anode current drawn by the lamp during operation If the anode current falls below the lower current limit the error message is generated Probable Causes e Lamp disconnected e Top foam removed while lamp is on e Defective lamp or non Agilent lamp e Defective VWM board e Defective power supply Suggested Actions V Ensure the lamp connector is seated firmly V Replace the top foam and turn ON the lamp V Exchange the lamp V Exchange the VWM board V Exchange the power supply 94 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Lamp Voltage Missing The lamp anode voltage is missing The processor continually monitors the anode voltage across the lamp during operation If the anode voltage falls below the lower limit the error message is generated Probable Causes e Defective or non Agilent lamp e Defective VWM board e Defective power supply Suggested Actions V Exchange the lamp V Exchange the VWM board V Exchange the power supply 1200 Series Variable Wavelength Detector Service Manual 95 7 96 Error Information Lamp Ignition Failed The lamp failed to ignite The processor monitors the lamp current during
31. grating on the shaft with the screw 3 Perform a wavelength calibration 4 Enter the Service Dialog see Service Dialog on page 124 and press Zero order Check the position of the image on the reference slit ws 5 If not correct Optimize the sample readings using the setscrew of mirror 2 through the hole in the optical unit cover see Optimizing The Sample Readings on page 196 adjust image horizontally by rotating the beam splitter If necessary unlock the reference aperture see Unlocking the Reference Aperture on page 197 1200 Series Variable Wavelength Detector Service M anual 191 12 Repair 6 Optimize the reference readings with the reference slit Install the standard slit and perform electronic see Optimizing the Reference Readings on page 198 calibrations see Installing the Standard Slit on page 199 TAA e Carefully replace the cover of the optical unit ae N A 192 1200 Series Variable Wavelength Detector Service M anual Repair 12 Replacing the Beam Splitter When required The beam splitter assembly might be replaced when the transmission of light is reduced and a lamp exchange a new lamp house lens or mirrors and grating do not improve the light throughput Tools required Screwdriver POZI 1 PT3 Wrench 1 4 1 5 inch Hexagonal wrench 1 5 mm A pointed pair of pliers Parts
32. is part of a complete Agilent 1200 Series system you can ensure optimum performance by installing the following configuration This configuration optimizes the system flow path ensuring minimum delay volume 34 1200 Series Variable Wavelength Detector Service M anual Installing the Detector 3 Solvent cabinet Vacuum degasser Pump Local User Interface Autosampler Column compartment Detector Figure5 Recommended Stack Configuration Front View 1200 Series Variable Wavelength Detector Service Manual 35 3 Installing the Detector Remote cable CAN Bus cable to local user interface CAN Bus cable Analog detector signal 1 or 2 outputs per detector LAN to LC ChemStation location depends on detector Figure6 Recommended Stack Configuration Rear View 36 1200 Series Variable Wavelength Detector Service M anual Installing the Detector 3 Installing the Detector Preparations Locate bench space Provide power connections Unpack the detector Parts required Detector Power cord for other cables see text below and on page 238 ChemStation and or Control Module G1323B 1 Install the LAN interface board in the detector if required see Replacing the Interface Board on page 150 2
33. limit the error message is generated Probable Causes e Leak sensor not connected to the VWM board e Defective leak sensor Suggested Actions V Ensure the leak sensor is connected correctly Y Exchange the leak sensor 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Leak Sensor Short The leak sensor in the detector has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable Causes e Defective leak sensor Suggested Actions V Exchange the leak sensor 1200 Series Variable Wavelength Detector Service M anual 89 7 Error Information Compensation Sensor Open The ambient compensation sensor NTC on the VWM board in the detector has failed open circuit The resistance across the temperature compensation sensor NTC on the VWM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable Causes e Defective VWM board Suggested Actions V Exchange the VWM board 90 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Com
34. micro Flow Cell Table 22 Semi micro Flow Cell Assembly Item Description Part Number Semi micro flow cell assembly 6 mm 5 ul 40 bar G1314 60083 1 Cell screw 79853 27200 Semi micro cell kit consisting of G1314 65056 two windows two 1 standard gaskets one 1 semi micro gasket and one 2 semi micro gasket 2 Conical springs pack of 10 79853 29100 3 Ring SST pack of 2 79853 22500 4 PTFE 1 standard gasket pack of 10 79853 68743 5 Quartz window pack of 2 79853 68742 6 Semi micro 1 gasket PTFE pack of 10 G1314 65057 7 Semi micro 2 gasket PTFE pack of 10 G1314 65058 Inlet capillary 400 mm long 0 12 mm i d 5021 1823 The semi micro 1 and 2 gaskets items 6 and 7 look very similar Do not mix them up 1200 Series Variable Wavelength Detector Service M anual 233 13 Parts and Materials for Maintenance NYO BR WNP 234 Cell screw Conical springs Ring SST Gasket 1 Quartz window Semi micro gasket 1 Semi micro gasket 2 Figure 56 Semi micro Flow Cell 1200 Series Variable Wavelength Detector Service M anual Parts and Materials for Maintenance 13 High Pressure Flow Cell Table 23 High Pressure Flow Cell Assembly Item Description Part Number High pressure flow cell 10 mm 14 ul 400 bar 61314 60082 Capillary column detector SST 380 mm lg 0 17 i d G1315 87311 one side not assembled 1 Cell screw 79853 27200 Cell kit Agilent comprises G1314 650
35. on Y Run the filter motor test to determine if the grating motor assembly is defective Exchange the filter motor assembly V Run the grating motor test to determine if the grating assembly is defective Exchange the grating motor assembly if required 1200 Series Variable Wavelength Detector Service Manual 99 7 Error Information Grating Filter M otor Test Failed The motor test has failed Test 0 Failed Filter motor Test 1 Failed Grating motor During the motor tests the detector moves the motor to the end position while monitoring the end position sensor If the end position is not found the error message is generated Probable Causes e Defective motor assembly Suggested Actions V Run the motor test again to confirm the motor assembly is defective Exchange the grating motor assembly if required 100 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Wavelength Check Failed The automatic wavelength check after lamp ignition has failed When the lamp is switched on the detector waits 1 minute to warm up the lamp Then a check of the deuterium emission line 656 nm via the reference diode is performed If the emission line is more than 3 nm away from 656 nm the error message is generated Probable Causes e Calibration incorrect Suggested Actions Y Recalibrate the detector 1200 Series Variable Wavelength Detector Service M anual 101 7 102 Error Information Fi
36. place it on the working bench Remove the front cover top cover and top foam section front part Install the Test Slit see Installing the Test Slit on page 185 Turn lamp ON g fal oo 1200 Series Variable Wavelength Detector Service M anual 187 12 Repair 2 Replace mirror 1 or 2 one at a time Do not fix the screws yet The mirrors must not be cleaned The surface will be damaged 3 Enter the Service Dialog see Service Dialog on page 124 and press Zero order The grating is moved into the zero order position 4 Locate the lamp image onto the reference slit hole a Sa SAN X reference D U 1 L 5 Position the lamp image onto the reference slit hole horizontally by rotating the mirror assembly and vertically using the setscrew on the mirror Fix the mirror 188 1200 Series Variable Wavelength Detector Service M anual Repair 12 6 Perform a wavelength calibration 8 Install the Standard Slit Installing the Standard Slit on 7 Do sample and reference optimization see Optimizing page 199 The Sample Readings on page 196 and see Optimizing the Reference Readings on page 198 1200 Series Variable Wavel
37. required Beam Splitter Assembly G1314 65013 includes test slit and seals for holes in cover Preparation for this procedure are 1 Remove the cover of the optical unit Locate the beam splitter assembly entrance slit holder and reference slit Turn OFF the lamp P y Switch OFF the module and disconnect the cables Remove the module from stack and place it on the working bench Remove the front cover top cover and top foam section front part Install the Test Slit see Installing the Test Slit on page 185 Turn lamp ON i F A Wf 1200 Series Variable Wavelength Detector Service M anual 193 12 Repair 2 Perform a wavelength calibration 6 Center the reference slit on the white image by moving the aperture up or down The image diameter is nearly equal 3 Carefully remove the cover of the optical unit Take care for ween P LETNE E ONET E es Pngaru as to the reference slit diameter the reference aperture handle 4 Enter the Service Dialog see Service Dialog on page 124 and press Zero order 5 Unlock the reference aperture see Unlocking the Reference Aperture on page 26 __ tefere
38. specification refer also to Performance Specifications on page 29 is based on these conditions Larger ambient temperature changes will result in larger drift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C hour 1 8 F hour Turbulences around one minute or less can be ignored Do not store ship or use your detector under conditions where temperature fluctuations could cause condensation within the detector Condensation will damage the system electronics If your detector was shipped in cold weather leave it in its box and allow it to warm up slowly to room temperature to avoid condensation 1200 Series Variable Wavelength Detector Service M anual 27 2 Site Requirements and Specifications Physical Specifications Table 2 Physical Specifications Type Specification Comments Weight 11 kg 25 Ibs Dimensions 140 x 345 x 435 mm height x width x depth 5 5 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 85 W 290 BTU Maximum Ambient operating temperature 0 55 C 32 131 F Ambient non operating temperature 40 70 C 4 158 F Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For st
39. stack Reconnect the cables and capillaries and turn ON the detector 1200 Series Variable Wavelength Detector Service M anual 181 12 Repair Exchanging the Filter Assembly When required The filter assembly may be replaced when the transmission of light is reduced and a lamp or source lens exchange does not improve the light throughput Tools required Screwdriver POZI 1 PT3 Parts required Filter assembly G1314 65010 Preparation for this procedure are Turn OFF the lamp Switch OFF the detector and disconnect the cables Remove the detector from stack and place it on the working bench Remove the front cover top cover and top foam section see Removing the Top Cover and Top Foams on page 163 1 Remove the top cover of the optical unit 182 1200 Series Variable Wavelength Detector Service M anual Repair 12 2 Locate the source lens and filter assembly Source lens Filter assembly assembly ANN di 3 Using a tweezers counter hold the filter assembly Unscrew and remove the top part of the filter assembly Note The filter may be cleaned with alcohol Do not scratch the filter nor get contaminations onto it 4 Observe the orientation of the filter assembly top part
40. status the upper right indicates the detector status green yellow red ml Line power switch with green light Figure 28 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 1200 Series Variable Wavelength Detector Service M anual 77 6 78 Troubleshooting and Diagnostics Detector Status Indicator The detector status indicator indicates one of four possible detector conditions When the status indicator is OFF and power switch light is on the detector is in a prerun condition and is ready to begin an analysis A green status indicator indicates the detector is performing an analysis run mode A yellow indicator indicates a not ready condition The detector is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the detector has detected an internal problem which affects correct operation of the detector Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis A red blinking indicator indicates that the module is in resident mode e g during update of main firmware
41. that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided 1200 Series Variable Wavelength Detector Service M anual Appendix A The operator of this instrument is advised that if the equipment is used in a manner not Specified in this manual the protection provided by the equipment may be impaired Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply
42. the bottom section of the optical unit see Repairs in the Bottom Section on page 201 Grating Assembly Beam Splitter Assembly Mirror 1 Filter Assembly Source Lens Entrance Slit Lamp Photo Diode SAM PLE Mirror 2 ADC SAM PLE ANC REFERENCE Figure 49 Location of Replaceable Parts in the Optical Unit Top Section 178 1200 Series Variable W avelength Detector Service M anual Repair 12 Exchanging the Source Lens Assembly When required The lens assembly may be replaced when the transmission of light is reduced and a lamp exchange does not improve the light throughput Tools required Screwdriver POZI 1 PT3 Hexagonal wrench 1 5 mm Parts required Source lens assembly G1314 65008 Preparation for this procedure are Turn OFF the lamp Switch OFF the detector and disconnect the cables Remove the detector from stack and place it on the working bench Remove the front cover top cover and top foam section see Removing the Top Cover and Top Foams on page 163 1 Remove the top cover of the optical unit
43. the cell compartment and all capillary connections Insert the flow cell Perform Wavelength Verification Calibration on page 155 to check the correct positioning of the flow cell Replace the front cover 1200 Series Variable Wavelength Detector Service M anual 141 11 Maintenance Repairing the Flow Cells When required Tools required Parts required Preparations If the flow cell needs repair due to leaks or contaminations Wrench 1 4 inch for capillary connections Hexagonal wrench 4mm Tooth picks See Standard Flow Cell on page 231 See Micro Flow Cell on page 232 See Semi micro Flow Cell on page 233 See High Pressure Flow Cell on page 235 Turn off the flow Remove the front cover Remove the flow cell see Exchanging a Flow Cell on page 139 The shown cell parts will differ depending upon the flow cell type For detailed parts schematics refer to above mentioned pages 1 Cell Screw 1 2 Conical Springs 3 Ring 1 PEEK 4 Gasket 1 small hole 5 Window Quartz 6 Gasket 2 large hole 7 Cell cover assembly 8 Ring 2 PEEK Figure 40 Standard Flow Cell 142 8 2 an 1 1200 Series Variable Wavelength Detector Service M anual Maintenance 11 Disassembling 1 Unscrew the cell screw using a 4 mm hexagonal wrench the Flow Cell 2 Remove the SST rings using a pair of tweezers 3 Use adhesive tape to remove the peek ring the w
44. the error message is generated Probable Causes e CAN cable disconnected e Defective CAN cable Defective main board in another module Suggested Actions V Ensure all the CAN cables are connected correctly V Switch off the system Restart the system and determine which module or modules are not recognized by the system V Ensure all CAN cables are installed correctly 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Leak A leak was detected in the detector The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the VWM board Probable Causes e Loose fittings e Broken capillary e Leaking flow cell Suggested Actions Y Ensure all fittings are tight V Exchange defective capillaries V Exchange flow cell components 1200 Series Variable Wavelength Detector Service M anual 87 7 88 Error Information Leak Sensor Open The leak sensor in the detector has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower
45. 0 TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low po 15 3 3 Gray Start Low x NC 4 Blue Shut down Low 2 NC 5 Pink Not connected T NC 6 Yellow Poweron High o 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1200 to 3396 Series II 3395A Integrators Use the cable 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1200 Series Variable Wavelength Detector Service M anual 265 15 Identifying Cables Agilent 1200 to 3396 Series III 3395B Integrators Connector Pin Pin SignalName Active 03396 61010 33XX Agilent 1200 TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low 15 3 3 Gray Start Low i NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Poweron High O0 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1200 to HP 1050 HP 1046A or Agilent 35900 A D Converters Connector Pin Pin Signal Name Active 5061 3378 HP 1050 Agilent 1200 TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 266 1200 Series
46. 062 8535 Fitting male PEEK quantity 1 0100 1516 Hex key 1 5 mm 8710 2393 Hex key 4mm 8710 2392 Wrench open end 1 4 5 16 inch 8710 0510 Wrench open end 4mm 8710 1534 238 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual Pa 6 ee 14 Parts for Repairs Overview of MainAssemblies 240 Optical Unit and Fan Assembly 242 Source Lens and Filter Assembly 245 Grating Assembly 246 Filter Assembly 248 Lens Assembly 250 Sheet Metal Kit 251 Plastic Parts 252 Leak Parts 253 Foam Parts 254 Power and Status Light Pipes 255 This chapter provides information on parts for repair ra Agilent Technologies 239 14 Parts for Repairs Overview of Main Assemblies Figure 60 Overview of Main Assemblies 1200 Series Variable Wavelength Detector Service M anual 240 Parts for Repairs 14 Table 27 Main Assemblies Item Description Part Number 1 Main board VWM for G1314C VWD SL 61314 66531 1 Main board VWM for G1314C VW D SL exchange part 61314 66531 1 Main board VWM for G1314B VWD G1314 66525 1 Main board VWM for G1314B VWD exchange part G1314 69525 Cable CAN assembly 5181 1516 Hexagonal nut for GPIB connector 0380 0643 Hexagonal nut for RS 232C connector 1251 7788 Nut for analog connector 2940 0256 Washer for analog connector 2190 0699 2 Power supply assembly additional power and status light parts see page 255 0950 2528 3 Interface board
47. 1 1 GB hee eSB 8 SBA8B8 Window VWD 4 Absorbance at 254 nm x axis 15 B min Type acquired y axis range 1 mAU J draw zero line T auto y adjust Offset 10 B Yo Fraction Colector p Method Settings IF Show Fraction collection ticks J Use method settings Apply to Method OK Cancel Help Figure 16 Edit Signal Plot Window 1200 Series Variable Wavelength Detector Service M anual 55 4 Using the Detector The Online Plot Figure 17 on page 56 shows both the pump pressure and the detector absorbance signals Pressing Adjust the signals can be reset to the offset value and Balance would do a balance on the detector Figure 17 Online Plot Window 13 If both baselines are stable set the Y range for the detector signal to 100 mAU NOTE If you start with a new UV lamp for the first time the lamp may show initial drift for some time burn in effect 56 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 14 Select the menu item RunControl gt Sample Info and enter information about this application Figure 18 on page 57 Press OK to leave this screen ASLAM M Sample Info x Offline Data Analysis Operator Name wolfgang r Data File Path JE CHEMSTATION Z DATAS x Subdirectory ISOTEST _ s Resume Injection Run Sequence F6 Pause Sequence Resume Sequence Manual Filename Stop Runji
48. 20 bit digital data direct photo current digitalization The data is transferred to the detector main board VWM The photo diode ADC boards VWAs are located close to the photo diodes 1200 Series Variable Wavelength Detector Service M anual Flow Cell Assembly Hardware Information 16 A variety of flow cell cartridges can be inserted using the same quick and simple mounting system Figure 74 Cartridge Type Flow Cell Table 41 Flow Cell Data STD Semi micro High Pressure Micro Maximum pressure 40 4 40 4 400 40 40 4 bar M Pa Path length 10 conical 6 conical 10 conical 5 mm Volume 14 5 14 1 ul Inlet i d 0 17 0 17 0 17 0 1 mm Inlet length 750 750 750 555 mm Outlet i d 0 25 0 25 0 25 0 25 mm Outlet length 120 120 120 120 mm Materials in contact with SST quartz SST quartz SST quartz SST quartz solvent PTFE PEEK PTFE Kapton PTFE 1200 Series Variable Wavelength Detector Service M anual 279 16 Hardware Information Detector M ain Board VWM This board controls all information and activities of all assemblies within the detector module The operator enters parameters changes modes and controls the module through interfaces CAN GPIB G1314B only or RS 232C connected to the user interfaces Depending on the version of the detector there are two main boards available G1314B VWD VWM board G1314 66525 G1314C VWD SL VWM board G1314 66531 high data rates requires firmware A 06 02 or ab
49. 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 not connected 14 not connected 15 5V Low 268 1200 Series Variable Wavelength Detector Service M anual Identifying Cables 15 Agilent 1200 to 3396 Integrators Connector Pin Pin SignalName BCD Digit 03396 60560 3392 3 Agilent 1200 1 1 BCD5 20 ea 2 2 BCD7 80 0 3 3 BCD 6 40 to 4 4 BCD 4 10 5 5 BCDO 1 s 6 6 BCD 3 8 ae 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 1200 Series Variable Wavelength Detector Service M anual 269 15 270 Identifying Cables Auxiliary Cable One end of this cable provides a modular plug to be connected to the Agilent 1200 Series vacuum degasser The other end is for general purpose Agilent 1200 Series Degasser to general purposes Connector Color Pin Signal Name G1322 61600 Agilent 1200 White 1 Ground Brown 2 Pressure signal Green 3 n Yellow 4 P Tm Grey 5 DC 5VIN Pink 6 Vent 1200 Series Variable Wavelength Detector Service M anual Identifying Cables 15 CAN Cable Both ends of this cable provide a modular plug to be connected to Agilent 1200 Series module s CAN bus connectors Agilent 1200 module to module 0 5 m 5181 1516 Agilent 1200 module to module 1 m 5181 1519 Agilent 1200 module to control module G1323 81600 1200 Series Variable Wavelength Detector Service M an
50. 46 Default Addresses Autosampler 28 Pump 22 FLD 23 VWD 24 Agilent 8453A 25 DAD MWD 26 Column Compartment 27 Autosampler RID Autosampler HP 1050 Pump HP 1050 VW D HP 1050 DAD HP 1050 28 29 18 10 17 CAN Interface The CAN is intermodule communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement 1200 Series Variable Wavelength Detector Service M anual 291 16 Hardware Information 292 Remote Interface The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective li
51. 5 Electrical Connections 19 Instrument Layout 21 Early Maintenance Feedback EMF 22 EMF Counter 22 Using the EMF Counters 22 2 Site Requirements and Specifications Site Requirements 26 Physical Specifications 28 Performance Specifications 29 3 Installing the Detector Unpacking the Detector 32 Optimizing the Stack Configuration 34 Installing the Detector 37 Flow Connections to the Detector 40 4 Using the Detector Setting up an Analysis 44 Before Using the System 44 Requirements and Conditions 46 1200 Series Variable Wavelength Detector Service M anual Optimization of the System 48 Preparing the HPLC System 49 Running the Sample and Verifying the Results 58 Special Settings of the Detector 59 Control Settings 59 Online Spectra 60 Scanning withthe VWD 61 Analog Output Settings 63 Special Setpoints 64 Peakwidth Settings 65 Optimizing the Detector 67 5 How to optimize the detector Optimizing the Detector Performance 70 Match the Flow Cell to the Column 70 Set the Detector Parameters 73 6 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 76 Status Indicators 77 Power Supply Indicator 77 Detector Status Indicator 78 User Interfaces 79 Agilent LC Diagnostic Software 80 7 Error Information What Are Error Messages 82 General Error Messages 83 Timeout 83 Shutdown 84 Remote Timeout 85 1200 Series Variable Wavelength Detector Service M anual Synchronization Lost 86 Leak 87
52. 54 two windows two KAPTON gaskets and two PEEK rings 2 Ring PEEK kit quantity 2 79853 68739 3 Window quartz kit quantity 2 79853 68734 4 Gasket kit KAPTON quantity 10 G1314 65055 1 Cell screw 1 2 Ring PEEK 3 Window quartz 4 Gasket KAPTON 5 Cell Cover Figure 57 High Pressure Flow Cell 1200 Series Variable Wavelength Detector Service M anual 235 13 Parts and Materials for Maintenance Cuvette Holder Table 24 Cuvette Holder Item Description Part Number Cuvette Holder G1314 60200 For information the use of the cuvette holder refer to Using the Cuvette Holder on page 145 Figure58 Cuvette Holder 236 1200 Series Variable Wavelength Detector Service M anual Parts and Materials for Maintenance 13 Leak Parts Table 25 Leak Parts Item Description Part Number 3 Leak funnel 5041 8388 Leak funnel holder 5041 8389 5 Clip 5041 8387 6 Corrugated tubing 120 mm lg re order 5 m 5062 2463 7 Corrugated tubing 1200 mm lg re order 5 m 5062 2463 Figure 59 Leak Parts 1200 Series Variable Wavelength Detector Service M anual 237 13 Parts and Materials for Maintenance Accessory Kit This kit contains some accessories and tools needed for the installation and repair of the detector Table 26 Accessory Kit Parts Description Part Number Accessory kit G1314 68705 Corrugated tubing to waste re order 5 m 5062 2463 Peek outlet capillary kit i d is 0 25 mm PEEK 5
53. Agilent 1200 Series Variable Wavelength Detector G1314B G1314C SL Service M anual Eee Agilent Technologies Notices Agilent Technologies Inc 2006 No part of this manual may be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G1314 90110 Edition Edition 02 2006 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany Manual Structure The Service M anual G1314 90110 English contains the complete information about the Agilent 1200 Series Variable Wavelength Detector It is available as Adobe Reader file PDF only The User Manual G1314 90010 English and its localized versions contain a subset of the Service M anual and is shipped with the detector in printed matter Latest versions of the manuals can be obtained from the Agilent web Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of
54. BCD External Contacts G1351 68701 LAN Communication Interface board G1369A G1369A or G1369 60001 4 Fan assembly additional parts see page 242 G1314 65004 5 Optical unit exchange part additional optical unit parts see page 242 G1314 69060 6 Deuterium lamp 61314 60100 8 Standard flow cell 10 mm 14 ul additional flow cell parts see page 231 G1314 60086 Micro flow cell 5mm 1 ul additional flow cell parts see page 232 61314 60081 High pressure flow cell 10 mm 14 ul additional flow cell parts see page 235 G1314 60082 Semi micro flow cell 6 mm 5 ul additional flow cell parts see page 233 G1314 60083 9 Leak sensor assembly 5061 3356 10 Leak handling parts see page 253 11 Plastic parts housing see page 252 12 Sheet metal parts see page 251 13 Foam parts see page 254 14 Power and status light parts see page 255 1200 Series Variable Wavelength Detector Service M anual 241 14 Parts for Repairs Optical Unit and Fan Assembly Certain repairs in the optical unit require special knowledge see Removing the Optical Unit on page 202 Table 28 Optical Unit and Fan Assembly Item Description Part Number 1 Optical unit exchange part 61314 69060 2 Deuterium lamp G1314 60100 3 Fan assembly without grille G1314 65004 Fan Grille not shown 3160 0397 Pin to clip the grille into fan used instead of screws 5042 8916 For lens assembly and filter top assembly see Source Lens and Filter Assembly on page 245 4 Bum
55. EST BOOT 1 1 RSVD SYS RSVD RSVD FC Switches 1 and 2 define which set of parameters for example for GPIB RS 232C and so on will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory 1200 Series Variable Wavelength Detector Service M anual 295 16 Hardware Information In the non volatile memory the parameters are kept regardless of whether you turn the instrument OFF and ON again They will be kept until the same set of parameters is changed and the power is reset All other previously stored configuration settings will still remain in the non volatile memory In this way you can store more than one set of parameters using the same 8 bit configuration switch twice for example for both GPIB and RS 232C GPIB Default Addresses If you just want to change the GPIB address and need a detailed procedure refer to the Installing Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses Table 50 Default Addresses for Agilent Series 1200 M odules Module Address Binary Address Pump 22 00010110 FLD 23 00010111 VWD 24 00011000 Agilent 8453A 25 00011101 DAD MWD 26 00011010 Column compartment 27 00011011 Autosampler 28 00011100 RID 29 00011101 where 0 means that the switch is down and 1 means that the switch is up 296 1200 Series Variable Wavelength Detector Service
56. G4208A Control M odule G1323B Wavelength Verification Re calibration Tests Lamp Intensity Tests Holmium Test Tests Cell Test Tests D A Converter Test Tests Dark Current Test Tests Filter Grating M otor Test Tests Test Chromatogram from command line Spectrum Blank Sample Holmium n a Service Dialog Tests Maintenance Diagnosis Diagnosis n a n a n a n a n a n a n a Tests Tests Tests n a n a n a n a Tests Tests Test m key interface provides passed fail information or a plot With the introduction of the Agilent 1200 Series of HPLC modules a user interface independent Diagnostic Software is available that can be used from any PC via LAN or RS 232 104 1200 Series Variable Wavelength Detector Service M anual Test Functions 8 Wavelength Verification Calibration Wavelength calibration of the detector is done using the zero order position and 656 nm emission line position of the deuterium lamp The calibration procedure involves two steps First the grating is calibrated on the zero order position The stepper motor step position where the zero order maximum is detected is stored in the detector Next the grating is calibrated against the deuterium emission line at 656 nm and the motor position at which the maximum occurs is stored in the detector In addition to the zero order and 656 nm alpha emission line calibration the
57. ION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met 1200 Series Variable Wavelength Detector Service M anual In This Manual This manual covers the Agilent 1200 Series Variable Wavelength Detectors G1314B Agilent 1200 Series VWD G1314C Agilent 1200 Series VWD SL Introduction to the Variable Wavelength Detector This chapter gives an introduction to the detector instrument overview and internal connectors Site Requirements and Specifications This chapter gives information on environmental requirements physical and performance specifications Installing the Detector This chapter describes the installation of the detector Using the Detector This chapter provides information on how to set up the detector for an analysis and explains the basic settings How to optimize the detector This chapter gives hints on how to select the detector parameters and the flow ce
58. Interface Board One board is required per Agilent 1200 stack It is recommended to add the LAN board to the detector with highest data rate The following cards can be used with the Agilent 1200 Series modules Table 44 LAN Boards Type Vendor Supported networks G1369A Agilent Technologies Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX 61369 60001 recommended for re ordering J 4106A Hewlett Packard Ethernet 802 3 RJ 45 10Base T J 4105A Hewlett Packard Token Ring 802 5 DB9 RJ 45 10Base T J 4100A Hewlett Packard Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 These cards may be no longer orderable M inimum firmware of these Hewlett Packard J etDirect cards is A 05 05 Recommended Cables Cross over network cable shielded 3 m long 5023 0203 for point to point connection Twisted pair network cable shielded 7 m long 5023 0202 for hub connections 1200 Series Variable Wavelength Detector Service M anual 289 16 Hardware Information Agilent 1200 Series Interfaces The Agilent 1200 Series modules provide the following interfaces Table 45 Agilent 1200 Series Interfaces Interface Type Pumps Autosampler DA Detector DA Detector VW Detector Thermostatted Vacuum MW Detector MW Detector RI Detector Column Degasse FL Detector G1315C G1365C Compartment r CAN Yes Yes Yes Yes Yes Yes No LAN on board No No No Yes No No No GPIB Yes Yes Yes No Yes No
59. J 12 RS 232C O O J 15 REMOTE S1 ie OM J2 ji J12 J15 J 208 J 17 GPIB J 150 Power supply J 201 Grating motor 203 Fan assembly J 204 Leak sensor J 205 Grating position J 207 Filter motor J 208 Analog output J 209 Filter position J 400 ADC board Sample J 401 ADC Board Reference J 502 Deuterium lamp J 203 J 502 S1 Configuration switch Q00 J17 mo H RR ii B Battery Figure 75 Board Layout and Connectors on VWM Board 284 1200 Series Variable Wavelength Detector Service M anual Hardware Information 16 Firmware Description The firmware of the instrument consists of two independent sections anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical in all Agilent 1200 Series modules Its properties are e the complete communication capabilities GPIB CAN LAN and RS 232C e memory management and e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities GPIB CAN LAN and RS 232C e memory management and e ability to update the firmware of the resident system In addition the main system comprises the instrument functions th
60. M anual Communication Settings for RS 232C Communication Hardware Information 16 The communication protocol used in this instrument supports only hardware handshake CTS RTS Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory Table 51 Communication Settings for RS 232C Communication Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baud rate Data Parity Bits Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Table 52 Baud Rate Settings Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 53 Data Bit Settings Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication 1200 Series Variable Wavelength Detector Service M anual 297 16 Hardware Information Forced Cold Start Settings 298 Table 54 Parity Settings Switches Parity 7 8 0 0 No Parity 1 0 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud
61. O1min 2s Figure 25 Peakwidth Setting 1200 Series Variable Wavelength Detector Service Manual 65 Using the Detector Table9 Peak Width Response Time Data Rate G1314B VW D Peak Width min Response Time sec Data Rate Hz lt 0 005 0 12 13 74 gt 0 005 0 12 13 74 gt 0 01 0 25 13 74 gt 0 025 0 5 13 74 gt 0 05 1 0 6 87 gt 0 10 2 0 3 43 gt 0 20 4 0 1 72 gt 0 40 8 0 0 86 Table 10 Peak Width Response Time Data Rate G1314C VWD SL Peak Width min Response Time sec Data Rate Hz lt 0 00125 lt 0 031 55 gt 0 00125 0 031 27 5 gt 0 0025 0 062 13 74 gt 0 005 0 12 13 74 gt 0 01 0 25 13 74 gt 0 025 0 5 13 74 gt 0 05 1 0 6 87 gt 0 10 2 0 3 43 gt 0 20 4 0 1 72 gt 0 40 8 0 0 86 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 Optimizing the Detector Additional theoretical information can be found in chapter How to optimize the detector on page 69 1200 Series Variable Wavelength Detector Service M anual 67 4 Using the Detector 68 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual U1 How to optimize the detector Optimizing the Detector Performance 70 This chapter gives hints on how to select the detector parameters and the flow cell ot Agilent Technologies 69 5 How to optimize the detector Optimizing the Detector Pe
62. Suggested Actions V Exchange the grating motor V Exchange the grating position sensor V Exchange the VWM board 1200 Series Variable Wavelength Detector Service M anual 117 8 Test Functions Filter M otor Test This control function is used to check the motion of second order cut off filter The cut off filter returns to original position at the end of this control function When to do the Test e if the holmium test fails Grating M otor Test Failed Probable Causes e Defective filter motor e Defective filter position sensor e Defective VWM board Suggested Actions Y Exchange the filter motor V Exchange the filter position sensor V Exchange the VWM board 118 1200 Series Variable Wavelength Detector Service M anual Test Functions 8 Test Chromatogram A pre defined test chromatogram on the VWM board is processed through ADC like normal signals from the diodes and can be used to check the ADC and the data handling system The signal is available at the analog output and on the GPIB The run time of the chromatogram is depending on the setting for response time peak width If no stop time is set the chromatogram will repeat continuously Table 15 Test Chromatogram Settings Response time Set Run Time 0 06 sec 0 8 min 0 12 sec 0 8 min 0 25 sec 0 8 min 0 50 sec 0 8 min 1 00 sec 1 6 min 2 00 sec 3 2 min 4 00 sec 6 4 min 8 00 sec 12 8 min Control M odule G1323B 1 Select the response time
63. Variable Wavelength Detector Service M anual Diagnosis Signals 9 Table 16 Diagnostic Signals on Analog Output continued Signal Analog Out Display 1V is equal to Analog output test zero offset 10 uV step Counts Sample diode current 220 nA 298 nA Reference diode current 220 nA 2927 nA Board Temperature 102 4 C displayed value C minus zero offset 1200 Series Variable Wavelength Detector Service M anual 123 9 Diagnosis Signals Service Dialog Available on Agilent ChemStation and G1323B Control Module This dialog interface displays the values on the screen only The analog signal will not be affected Some of the signals can be routed to the analog output selectable in the VWD Settings Analog screen On the Control Module G1323B it can be accessed from System Tests VWD Then press m menu and select Service Selection list of Actual values of signal 1 and 2 signal land 2 Position grating on zero order line YWD Service Dialog Signal 1 Absorbance Units 0 001044 Signal 2 Absorbance Units 0 001 043 Grating Filter Gain Sample Gain Reference Position fieaa7 Position otf Actual ea Actual jie rer co g Step Width Position of grating Position of filter Actual gain setting Actual gain setting drive drive for sample for reference Figure 37 Service Dialog 124 1200 Series Variable Wavelength Detector Service M anual Table 17 Diagnosti
64. Wavelength Detector Service M anual Maintenance 11 Using the Cuvette Holder When required If your own standard should be used to checkout the instrument Tools required None Parts required Cuvette Holder G1314 60200 Cuvette with the standard e g NIST certified holmium oxide sample This cuvette holder can be placed instead of a flow cell in the variable wavelength detector Standard cuvettes with standards in it for example National Institute of Standards amp Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications Figure 41 Cuvette Holder 1200 Series Variable Wavelength Detector Service M anual 145 11 Maintenance Preparation for this procedure are Remove the normal flow cell e Have cuvette with standard available IL Locate the cuvette holder on the desk 2 Unscrew the bracket 3 Insert the cuvette with the sample into the holder The clear side of the cuvette must be visible LC LIU So v4 7 Light path Clear side Next steps Turn the lamp ON e J e Give the lamp more than 10 minutes to warm up e Reset the lamp counter as described in the User Interface documentation Perform Wavelength Verification Calibration on page 155 to check the correct positioning of the lamp 146 1200 Series Variable Wavelength Detector Service M anual Maintenance 11 4 Replace the b
65. agnosis VWD Details view Temperature ER hod Array Detector lemecretuer Melony Pal xj Tem petatens C ad detector ON lamp turned off for a moment IN detector ON lamp turned ON detector ON lamp OFF 2 a a 0 Tes Oo Figure 38 Board Temperature internal detector buffer 1200 Series Variable Wavelength Detector Service M anual 127 9 Diagnosis Signals Leak Status Provides status of PTC and NTC sensors See Table 17 on page 125 PTC or NTC voltage current The actual voltage current of the PTC leak sensor or NTC reference sensor is provided 128 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector o Service M anual e e e 10 Maintenance and Repair e e j Introduction to Maintenance and Repair 130 e Warnings and Cautions 131 Cleaning the Detector 132 Using the ESD Strap 133 This chapter provides general information on maintenance and repair of the detector ot Agilent Technologies 129 10 Maintenance and Repair Introduction to Maintenance and Repair Simple Repairs Maintenance The detector is designed for easy repair The most frequent repairs such as lamp change and flow cell change can be done from the front of the detector with the detector in place in the system stack These repairs are described in Maintenance on page 135 part of the User Manual and Service Manual Exchanging Internal P
66. al and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 1200 Series Variable Wavelength Detector Service M anual 21 1 Introduction to the Variable Wavelength Detector Early Maintenance Feedback EM F 22 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should b
67. allows to add an interface board to the modules Table 42 Optional Interface Boards Description Part Number BCD Board G1351 68701 Fuse 250 mA four are on the board 2110 0004 LAN Communication Interface Board G1369A or G1369 60001 BCD Board The BCD board provides a BCD output for the bottle number of the Agilent 1200 Series autosampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused Board identification 2 Processor BCD register BN 12 BCD lt T interface i connector Line driver 250mA External External contact contacts 4x connector Figure 77 Block Diagram BCD Board 1200 Series Variable Wavelength Detector Service M anual 287 16 Hardware Information There are general purpose cables available to connect the BCD output see BCD Cables on page 268 and the external outputs see External Contact Cable on page 272 to external devices Table 43 Detailed connector layout 1200 Pin Signal name BCD digit 1 BCD5 20 2 BCD7 80 3 BCD 6 40 4 BCD 4 10 5 BCDO0 1 6 BCD 3 8 7 BCD 2 4 8 BCD 1 2 9 Digital ground 10 BCD 11 800 11 BCD 10 400 12 BCD 9 200 13 BCD 8 100 15 5V Low 288 1200 Series Variable Wavelength Detector Service M anual Hardware Information 16 LAN Communication
68. als additional diagnostics 54 diagnostic 76 122 site requirements 26 bench space 27 environment 27 power considerations 26 power cords 26 solvent information 311 sound emission 309 source lens assembly 17 276 special setpoints 54 64 special settings 59 specifications performance 29 physical 28 spectra online 60 stable baseline 51 stack configuration 34 36 rear view 36 standards 28 Status indicators 76 78 stepper motor 278 stop time post time 54 stop flow condition 60 switch configuration settings 295 safety 283 T test functions 76 103 tests 656 nm calibration 106 DAC digital to analog 116 dark current 114 diagnostic signals 122 125 grating motor 117 holmium oxide 109 holmium oxide holmium oxide test 156 intensity of deuterium lamp 112 intensity of lamp 153 photocurrent readings 108 test chromatogram 119 wavelength calibration 105 155 zero order calibration 106 time table 54 troubleshooting Agilent LC diagnostic software 80 available tests vs interface 104 diagnostic signals 76 error messages 76 82 overview 76 status indicator 78 status indicators 76 77 test functions 76 103 user interfaces 79 turn on automatic 59 U unpacking 32 user interfaces ChemStation Control M odule Instant Pilot 79 using additional signals diagnostics 54 Agilent ChemStation 49 analog output range 59 analog output settings 63 atpoweron 59 at
69. als in contact with SST quartz SST quartz SST quartz SST quartz solvent PTFE PEEK PTFE Kapton PTFE 16 1200 Series Variable Wavelength Detector Service M anual Introduction to the Variable Wavelength Detector 1 Lamp The light source for the UV wavelength range is a deuterium lamp As a result of plasma discharge in a low pressure deuterium gas the lamp emits light over the 190 to 600 nm wavelength range Source Lens Assembly The source lens receives the light from the deuterium lamp and focuses it onto the entrance slit Entrance Slit Assembly The entrance slit assembly has an exchangeable slit The standard one has a 1 mm slit For replacement and calibration purposes to optimize the alignment a slit with a hole is needed Filter Assembly The filter assembly is electromechanically actuated During wavelength calibrations it moves into the light path The filter assembly has two filters installed and is processor controlled OPEN nothing in light path CUTOFF cut off filter in light path at gt 370 nm HOLMIUM holmium oxide filter for wavelength check A photo sensor determines the correct position Cutoff filter L Holmium oxide filter with cutoff filter Figure3 Filter Assembly 1200 Series Variable Wavelength Detector Service M anual 17 1 18 Introduction to the Variable Wavelength Detector Mirror Assemblies M1 and M2 The instrument contains two spherical mirrors M1 and M2 The beam adju
70. aning of 82 remote timeout 85 shutdown 84 synchronization lost 86 timeout 83 wavelength check failed 101 1200 Series Variable Wavelength Detector Service M anual method error 59 load 52 mirror assemblies 18 277 contaminated 177 M PS main power supply 300 negative absorbance margins 64 noise short term 29 0 online plot 56 online spectra 60 operating temperature 28 optical unit and fan parts 242 beam splitter 278 beam splitter assembly 18 components 276 cutoff filter 277 deuterium lamp 276 entrance slit assembly 17 277 filter 17 filter assembly 17 flow cell 16 279 grating assembly 18 278 holmium oxide filter 277 lamp 17 layout of components 276 mirrors 18 277 photodiode assemblies 18 278 photodiode boards 18 278 source lens assembly 17 276 stepper motor 278 optimization detector performance 70 of the system 48 overview optical path 15 276 optical system 15 276 system overview 15 276 P parameters detector 53 parts and materials for maintenance 229 for repair 239 parts cable 257 analog 260 APG remote 263 auxiliary 270 BCD 268 CAN 271 external contact 272 LAN 274 overview 258 RS 232C 273 parts for maintenance accessory kit 238 cuvette holder 236 high pressure flow cell 235 leak panels 237 micro flow cell 232 overview of maintenance parts 230 semimicro flow cell 233 standard flow cell 231 parts for repair filter assemb
71. anual Repair 12 Contaminated Mirrors and Grating Under some specific environmental conditions it might happen that the efficiency of the mirrors and the grating is decreasing faster than normal This results in low photo current readings on sample and reference side Contaminated mirrors and the grating should be replaced As a temporary solution the following procedure might be performed for mirrors only until new mirrors are available 1 Note the photo current values of reference and sample side 2 Open the optical unit and observe the mirrors and the grating 3 Ifthe mirror or grating shows a clouded surface contaminations you can try to clean the surface carefully within the instrument Use HPLC grade water isopropanol or ethanol might depend on the contaminations and a lint free tissue 4 Do not bring to much stress onto the mirror surface to avoid scratches or misalignment 5 Take care that the mirror surface has no deposits left from the cleaning process 6 Close the optical unit and perform zero order and wavelength calibration 7 Note the photo current values of reference and sample side and compare with step 1 1200 Series Variable Wavelength Detector Service M anual 177 12 Repair Repairs in the Top Section Following parts can be replaced in the top section e Mirror 1 and 2 e Grating Assembly e Filter Assembly e Beam Splitter Assembly e Photo diodes e Lens Assembly For replacements in
72. arts Repair Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the detector from the stack removing the covers and disassembling the detector The security lever at the power input socket prevents that the detector cover is taken off when line power is still connected These repairs are described in Repair on page 159 part of the Service Manual 130 1200 Series Variable Wavelength Detector Service M anual Maintenance and Repair 10 Warnings and Cautions WARNING To prevent personal injury the power cable must be removed from the instrument before opening the detector cover Do not connect the power cable to the detector while the covers are removed WARNING To prevent personal injury be careful when getting in contact with sharp metal areas WARNING When working with solvents please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD In order to prevent damage always use an ESD protection for example the ESD wrist strap from the accessory kit when handling electronic boards and components see Using the ESD Strap on page 133 Eye damage may result from direc
73. as options with the detector The female end of the power cords is identical It plugs into the power input socket at the rear of the detector The male end of each power cord is different and designed to match the wall socket of a particular country or region Never operate your instrumentation from a power outlet that has no ground connection Never use a power cord other than the Agilent Technologies power cord designed for your region 26 1200 Series Variable Wavelength Detector Service M anual Site Requirements and Specifications 2 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Bench Space The detector dimensions and weight see Table 2 on page 28 allow to place the instrument on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches in the rear for air circulation and electric connections If the bench should carry a Agilent 1200 Series system make sure that the bench is designed to bear the weight of all modules The detector should be operated in a horizontal position Environment Your detector will work within specifications at ambient temperatures and relative humidity as described in Table 2 on page 28 ASTM drift tests require a temperature change below 2 C hour 3 6 F hour measured over one hour period Our published drift
74. at are divided into common functions like e run synchronization through APG remote e error handling e diagnostic functions and so on or module specific functions like e internal events such as lamp control grating and filter movements e raw data collection and conversion to absorbance and so on 1200 Series Variable Wavelength Detector Service M anual 285 16 Hardware Information Firmware Updates Firmware updates can be done using your user interface e instant pilot G4208A with files from a USB memory stick or e handheld control module G1323 with files from a PC card or e aPC Firmware Update Tool with files from hard disk or CD ROM The file naming conventions are 1314B_A602_zz dlb where xxxxx is the product number e g 1314B for the G1314B VWD and vvvv is the revision number for example A602 is revision A 06 02 and ZZ is the build number of the firmware For instructions refer to the documentation provided with the Firmware Upgrade Tools provided on the Agilent web Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main FW update Resident System Main System Resident FW update Figure 76 Firmware Update M echanism 286 1200 Series Variable Wavelength Detector Service M anual Hardware Information 16 Optional Interface Boards The Agilent 1200 Series modules have one optional board slot that
75. at the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents e g Acetonitrile or Methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents e Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on e High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl13 Og 2COClg 2HCI1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol 1200 Series Variable Wavelength Detector Service M anual 311 A Appendix e Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid
76. ation Calibration Wavelength calibration of the detector is done using the zero order position and 656 nm emission line position of the deuterium lamp The calibration procedure involves two steps First the grating is calibrated on the zero order position The stepper motor step position where the zero order maximum is detected is stored in the detector Next the grating is calibrated against the deuterium emission line at 656 nm and the motor position at which the maximum occurs is stored in the detector In addition to the zero order and 656 nm alpha emission line calibration the beta emission line at 486 nm and the three holmium lines are used for the complete wavelength calibration process These holmium lines are at 360 8 nm 418 5 nm and 536 4 nm WD Wavelength Calibration System 2 Calibration history Deviation Zero order Ref lines Time Date 8 8nm 0 8mm 21 55 49 07 02 1970 Deviation Hitit 0 0nm Olnm 11 40 47 04 01 2006 12nm Zero order 12nm Max Deviation UNE EE prrert 12nm Reference lines 12nm Zero order deviation is 0 0nm Max deviation reference lines 0 0nm OK Cancel Calibration settings equal to measured one no calibration necessary Figure 45 Wavelength Verification Calibration The wavelength verification calibration takes about 2 5 minutes and is disabled within the first 10 minutes after ignition of the lamp because initial drift may distort the measurement When the la
77. c Signals Diagnosis Signals 9 Signal Display Analog Output Absorbance AU 1LAU V Sample signal without reference AU 1LAU V Reference signal without sample AU 1AU V Sample raw data Counts 271 counts V unfiltered and not logarithmic Reference raw data Counts 221 counts V unfiltered and not logarithmic Analog output test Counts zero offset 10 uV step Sample diode current nA 220 nA 298 Reference diode current nA 220 nA 298 Sample preamplifier gain gain0 5 Reference preamplifier gain gain 0 5 Lamp anode voltage V Lamp anode current mA Lamp heater voltage V Lamp heater current A Grating motor steps Steps Grating position sensor ON OFF Filter motor position 0 4 Filter position sensor ON OFF Board Temperature 2 102 4 C displayed value minus zero offset PTC current mA Leak status 1 0 5 PTC leak sensor voltage mV NTC reference sensor voltage V 1200 Series Variable Wavelength Detector Service M anual 125 9 126 Diagnosis Signals for more information see Diagnostic Signal Output on page 122 0 in Sensor position 1 OFF lt 370 nm 2 cutoff filter in 3 unused 4 holmium filter in 1 warm up 0 OK 1 leak 2 NTC short 3 NTC open 4 PTC short 5 PTC open Signal Descriptions Constant Zero Offset This sets the output signal to zero 0 mV Absorbance This is the normal signal sample plus reference defined by user wavel
78. ces 290 Analog Signal Output 291 GPIB Interface 291 CAN Interface 291 Remote Interface 292 RS 232C 293 Setting the 8 bit Configuration Switch 295 GPIB Default Addresses 296 Communication Settings for RS 232C Communication 297 Forced Cold Start Settings 298 Stay Resident Settings 299 Main Power Supply Assembly 300 General Safety Information 304 Lithium Batteries Information 307 Radio Interference 308 Sound Emission 309 UV Radiation 310 Solvent Information 311 Declaration of Conformity for HOX2 Filter 313 Agilent Technologies on Internet 314 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 1 Introduction to the Variable Wavelength Detector Introduction to the Detector 14 Optical System Overview 15 Flow Cell 16 Electrical Connections 19 Instrument Layout 21 Early Maintenance Feedback EMF 22 This chapter gives an introduction to the detector instrument overview and internal connectors a Agilent Technologies 13 1 Introduction to the Variable Wavelength Detector Introduction to the Detector The Agilent 1200 Series variable wavelength detector is designed for highest optical performance GLP compliance and easy maintenance with e higher data rate 27 55Hz for fast HPLC with G1314C VWD SL see Set the Detector Parameters on page 73 e deuterium lamp for highest intensity and lowest detection limit over a wavelength range of
79. ctor 4 Scanning with the VW D j eF Access to the scan feature is only possible during run 1 Set up a run Start a run 3 While running on the baseline select from the menu Instrument More VWD Blank Scan see Figure 22 on page 61 A background scan is stored in the memory Set up Injector Navigation Bar Set up Column Thermostat Set up VWD Signal EK C CHEMS More T AFCDE More Injector SE BATCH More Column Thermostat EE DEF LEAT Step 1 Blank Scan scan of the background Gi DeNor SB nsrpe Snapshot S Loapt System On ROBUS System Off solvent is stored in the memory Sample Scan Blank Scan Step 2 Sample Scan scan of the peak of interest is taken while the peak stays in the l a wal Sern eh oe flow cell stop flow condition seqsu Columns Online Spectrum Sample Scan minus ER stati Blank Scan Configure 1100 1200 Access Figure 22 Taking Online Spectra 4 When the peak of interest enters the flow cell stop the flow set flow rate to zero or open the purge valve and wait a few moments to stabilize the concentration 1200 Series Variable Wavelength Detector Service M anual 61 4 Using the Detector Turning off the pump would stop the run and no access to the sample scan is possible 5 Select from the menu Instrument More VWD Sample Scan A sample scan is taken in the range defined under Special Setpoints on page 64
80. d e Defective VWM board Suggested Actions V Exchange the reference or sample diode V Exchange sample or reference VWA board V Exchange the VWM board 1200 Series Variable Wavelength Detector Service M anual 115 8 Test Functions DAC Test This test determines correct operation of the digital analog converter DAC The test applies an AC voltage 10 uV to the DC output of the DAC The DAC output is connected to the analog output connector at the rear of the detector ADC1 A ADC CHANNEL A 6131 4A DACOO000 D 76 583 76 585 76 584 76 582 N ODS an SA SOARS GARR RL GR GRA eee eee ane 0 2 0 4 0 6 os 4 1 2 1 4 16 18 mir Figure 34 DAC Test When to do the Test e if the detector signal is noisy or missing Interpreting the Results The noise on the step should be less than 3 uV Probable Causes e Defective VWM board Suggested Actions V Exchange the VWM board 116 1200 Series Variable Wavelength Detector Service M anual Test Functions 8 Grating M otor Test This test moves the grating motor to the end position and displays the motor position step difference This checks the movement of the grating drive mechanism When to do the Test e if recalibration cannot be done successfully or e if the holmium test fails Interpreting the Results Grating M otor Test Failed Probable Causes e Defective grating motor e Defective grating position sensor e Defective VWM board
81. detector att Agilent Technologies 31 3 Installing the Detector Unpacking the Detector Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the detector may have been damaged during shipment CAUTION If there are signs of damage please do not attempt to install the detector Delivery Checklist Ensure all parts and materials have been delivered with the detector The delivery checklist is shown below Please report missing or damaged parts to your local Agilent Technologies sales and service office Table 4 Variable Wavelength Detector Checklist Description Quantity Variable wavelength detector 1 Power cable 1 Flow cell As ordered User M anual 1 Accessory kit see Table 5 on page 33 1 32 1200 Series Variable Wavelength Detector Service M anual Installing the Detector 3 Detector Accessory Kit Contents Table5 Accessory Kit Contents Description Part Number Quantity Accessory kit G1314 68705 CAN cable 0 5m 5181 1516 1 PEEK outlet capillary kit 5062 8535 1 Fitting male PEEK 0100 1516 1 Hex key 1 5 mm 8710 2393 1 Hex key 4mm 8710 2392 1 Wrench open end 1 4 5 16 inch 8710 0510 1 Wrench open end 4mm 8710 1534 1 1200 Series Variable Wavelength Detector Service Manual 33 3 Installing the Detector Optimizing the Stack Configuration If your detector
82. ds 13 74 Hz G1314B G1314C 0 05 minutes 1 second 6 87 Hz G1314B G1314C 0 1 minutes 2 seconds 3 43 Hz G1314B G1314C 0 2 minutes 4 seconds 1 72 Hz G1314B G1314C 0 4 minutes 8 seconds 0 86 Hz G1314B G1314C 2 Choose the sample wavelength at a longer wavelength than the cut off wavelength of the mobile phase at a wavelength where the analytes have strong absorptivity if you want to get the lowest possible detection limit at a wavelength with moderate absorptivity if you work with high concentrations and preferably where the spectrum is flat for better linearity 3 Consider to use time programming to further optimization The G1314C VW D SL can be operated with a G1323B just in standard mode as G1314B no higher data rate selection is available 1200 Series Variable Wavelength Detector Service M anual 73 5 How to optimize the detector 74 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 6 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 76 Status Indicators 77 User Interfaces 79 Agilent LC Diagnostic Software 80 Overview about the troubleshooting and diagnostic features ot Agilent Technologies 75 6 Troubleshooting and Diagnostics Overview of the Detector s Indicators and Test Functions 76 Status Indicators The detector is provided with two status indicators which indicate
83. e scheduled EMF Counter The detector module provides a EMF counter for the lamp The counter increments with lamp use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded The counter can be reset to zero after the lamp is exchanged The detector provides the following EMF counters e Deuterium Lamp On Time Using the EMF Counters The user settable EMF limits for the EMF counter enables the early maintenance feedback to be adapted to specific user requirements The useful lamp burn time is dependent on the requirements for the analysis high or low sensitivity analysis wavelength and so on therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EM F Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary take note of the values displayed by lamp counters Enter these values or values slightly less than the displayed values 1200 Series Variable Wavelength Detector Service M anual Introduction to the Variable Wavelength Detector 1 as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1200 S
84. e the entrance slit holder 5 Return to the procedure you are coming from 4 Carefully insert the test slit with round hole into the entrance slit holder starting at the arrow side first The slit must sit flat on the holder with the white side towards the incoming light 1200 Series Variable Wavelength Detector Service M anual 186 Repair 12 Exchanging the Mirror 1 or 2 Assembly When required The mirror assemblies might be replaced when the transmission of light is reduced and a lamp exchange or new lamp house lens do not improve the light throughput Tools required Screwdriver POZI 1 PT3 Wrench 1 4 1 5inch Hexagonal wrench 1 5 mm A pointed pair of pliers Parts required Mirror 1 Assembly G1314 65012 Mirror 2 Assembly same as Mirror 1 If mirrors show signs of contaminations read Contaminated M irrors and Grating on page 177 first Do exchange only one mirror at a time Preparation for this procedure are Locate the mirror 1 or 2 entrance slit holder and Tum OFF the lamp reference slit Switch OFF the module and disconnect the cables Remove the module from stack and
85. e within 3 nm This measurement is taken at the reference side only to have no influence from flow cell condition If not correct then a message for recalibration is displayed Intensity check at 250 nm without cutoff filter value 1 at 250 nm with cutoff filter value 2 The result should be value 2 lt value 1 16 If not OK the cutoff filter does not work correctly Restore the set wavelength position The detector should now be ready condition 1200 Series Variable Wavelength Detector Service M anual 107 8 108 Test Functions Checking the Photocurrent To identify problems with the flow cell contaminated windows air bubbles and so on you can use raw data from the sample and reference photodiode unfiltered and not logarithmic The screen of the VW D Tests Control M odule G1323B on the control module in Figure 30 shows the wavelength and photo current information Lamp ice 0 OORT Ready VW Tesis RY Function Sqr ER ete wee Wavelength Raw Sample 3604 counts 254 nm Raw Reference 4044 1 2 counts actual wavelength actual intensity counts raw data Figure 30 Photocurrent Readings on Control Module With a clean cell the counts for sample and reference are in the same range If like in this example the sample side shows much less the flow cell might have a problem due to Y dirty windows V absorbing solvents compounds Y not correctly mounted flow cell or Y a
86. ed for internal Agilent 1200 Series module data transfer and synchronization e One analog output provides signals for integrators or data handling systems e The interface board slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch see Communication Settings for RS 232C Communication on page 297 See your software documentation for further information e The power input socket accepts a line voltage of 100 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power consumption is 220 VA There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents the module cover from being taken off when line power is still connected WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Se
87. embly 210 1200 Series Variable Wavelength Detector Service M anual Repair 12 10 Route the cables through the openings 11 Replace the cover 12 Place the optical unit in normal position and replace the 13 Continue as described in Exchanging the Grating grating Assembly on page 190 1200 Series Variable Wavelength Detector Service M anual 211 12 Repair Replacing the Filter M otor or Position Sensor When required The filter motor or the position sensor might be replaced when the motor does not turn or the filter position is not sensed correctly Tools required Screwdriver POZI 1 PT3 Wrench 1 4 1 5inch Hexagonal wrench 1 5 mm a pointed pair of pliers Parts required Filter M otor Assembly G1314 65011 includes plate and filter bottom Filter Position Sensor G1314 65006 Preparation for this procedure are 1 Place the optical unit on its top and remove the cover to have access to the bottom parts Install the Test Slit see Installing the Test Slit on page 185 Remove the optical unit from the instrument see Removing the Optical Unit on page 202 The top cover of the optical unit is removed 212 1200 Series Variable Wavelength Detecto
88. ength Sample signal without reference This is the normal signal defined by user wavelength without the reference No influence from the reference diode Reference signal without sample This is the normal signal defined by user wavelength without the sample No influence from the sample diode Sample or reference raw data The sample or reference diode s data is processed without filtering and take the logarithm Analog output test This test adds to the used zero offset setting a DC voltages of 10 uV in cycles of 12 seconds This 10 uV step is equal to 1 x 10 AU and can be used to check the noise on a recording device See DAC Test on page 116 Sample or reference diode current Shows the diodes current 1200 Series Variable W avelength Detector Service M anual Diagnosis Signals 9 Sample or reference gain This function shows the current the gain setting Lamp anode heater voltages and currents Provides the information of actual voltages and currents of the lamp s anode and filament Grating or Filter M otor motor steps Provides the step numbers of grating or filter motor settings Grating or Filter position sensor Checks the movement of grating or filter Board Temperature An on board temperature sensor on the VWM board provides the actual temperature on the board This information is running continuously into a buffer from which it can be retrieved as last 12 hours plot Agilent ChemStation Di
89. ength Detector Service M anual 189 12 Repair Exchanging the Grating Assembly When required The grating assembly might be replaced when the transmission of light is reduced and a lamp exchange or new lens or new mirrors 1 2 do not improve the light throughput Tools required Screwdriver POZI 1 PT3 Wrench 1 4 1 5inch Hexagonal wrench 1 5 mm A pointed pair of pliers Parts required Grating Assembly G1314 65007 includes test slit and seals for holes in cover If grating shows signs of contaminations read Contaminated Mirrors and Grating on page 177 first Preparation for this procedure are 1 Remove the cover of the optical unit Locate the grating assembly entrance slit holder and reference slit Turn OFF the lamp Switch OFF the module and disconnect the cables Remove the module from stack and place it on the working bench Remove the front cover top cover and top foam section front part see Reference Handbook Install the Test Slit see Installing the Test Slit on page 185 Turn lamp ON 190 1200 Series Variable Wavelength Detector Service M anual Repair 12 2 Remove the grating and reassemble new grating Fix the
90. equired Screwdriver POZI 1 PT3 Parts required Deuterium lamp G1314 60100 If you want to use the Agilent DAD lamp instead of the VW D lamp you have to change the lamp settings in the VW D Configuration to lamp type 2140 0590 This ensures that the DAD lamp s filament heating is operated like in the DAD The instrument specifications are based on the VWD lamp If the detector has been in use the lamp may be hot If so wait five minutes for lamp to cool down Turn the lamp OFF Preparations for this procedure 1 Press the release buttons and remove the front cover to have access to the lamp area 1200 Series Variable Wavelength Detector Service M anual 137 11 Maintenance 4 Unscrew the lamp cover and remove it 5 Unscrew disconnect and replace the lamp Insert fix and reconnect the lamp id n F i J TTT d 7 Replace the front cover Next steps e e Turn the lamp ON Give the lamp more than 10 minutes to warm up e Reset the lamp counter as described in the User Interface documentation Perform Wavelength Verification Calibration on page 155 to check the correct positioning of the lamp 138 1200 Series Variable Wavelength Detector Service M anual Maintena
91. er top cover and top foam section see Removing the Top Cover and Top Foams on page 163 5 Remove the optical unit see Removing the Optical Unit on page 202 6 Remove the leak base from the cabinet bottom by 7 Disconnect the leak sensor assembly on the processor unlocking it with a flat blade on the right and left side board and remove it from the leak panel 216 1200 Series Variable W avelength Detector Service M anual Repair 12 8 Replace the leak sensor assembly into the leak panel and route the cable through the cabinet and foam channel Reconnect it to the main board Lh D 9 Replace the leak base by pressing it into the bottom of the cabinet 6 Replace the optical unit see Installing the Optical Unit on page 223 7 Replace the front cover top cover and top foam section see Installing the Foam and the Top Cover on page 225 8 Replace the detector into the stack 9 Reconnect the cables and capillaries 10 Turn ON the detector 1200 Series Variable Wavelength Detector Service M anual 217 12 Repair Exchanging the Power Supply When required If defective Tools required Screwdriver POZI 1 PT3 Wrench 1 4 inch Wrench 5 mm 7 mm and 15 mm Parts required Power supply 0950 2528 The repair level of the power supply assembly is exchanging the complete assembly No serviceable parts are inside Turn OFF the lamp Switch OFF the
92. er 193 Exchanging the Grating Assembly 190 Optimizing The Sample Readings 196 Unlocking the Reference Aperture 197 Optimizing the Reference Readings 198 Installing the Standard Slit 199 Repairs inthe Bottom Section 201 Removing the Optical Unit 202 Exchanging a Photo Diode and or ADC Board Assembly 204 Replacing the Grating Motor or Position Sensor 208 Replacing the Filter M otor or Position Sensor 212 Exchanging the Leak Sensor 216 25 Agilent Technologies 159 12 Repair Exchanging the Power Supply 218 Replacing Status Light Pipe 222 Installing the Optical Unit 223 Installing the Foam and the Top Cover 225 Assembling the Main Cover 227 This chapter gives instructions on how to repair the detector 160 1200 Series Variable Wavelength Detector Service M anual Repair 12 Cautions and Warnings The following procedures require opening the main cover of the detector Alw ays ensure the detector is disconnected from the line power when the main cover is removed The security lever at the power input socket prevents the detector cover from being taken off when line power is still connected To disconnect the detector from line unplug the power cord The power supply still uses some power even if the switch on the front panel is turned off When working with solvents please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safe
93. eries VWD it is necessary to have more precise information on path lengths of the VWD flow cells The correct response is expected response correction factor Please find below the details of the Agilent 1200 Series VWD flow cells Table 11 Correction factors for Agilent 1200 Series VWD flow cells Flow cell type Cell Part number Path length Path length Correction volume nominal actual factor Standard flow cell 14ul G1314 60086 10mm 10 15 0 19 mm 10 10 15 Semi micro flow cell 5 ul G1314 60083 6mm 6 10 0 19 mm 6 6 10 Micro flow cell lul G1314 60081 5mm 4 80 0 19 mm 5 4 8 High Pressure flow cell 14 ul 61314 60082 10 mm 10 00 0 19 mm 6 5 75 However you have to be aware that there are additional tolerance of gasket thickness and its compression ratio which is supposed to be very small in comparison with the machining tolerance 72 1200 Series Variable Wavelength Detector Service M anual How to optimize the detector 5 Set the Detector Parameters 1 Set peakwidth as close as possible to the width at half height of a narrow peak of interest Table 12 Peakwidth Settings Peakwidth at half height Rise time 10 90 Data rate Module lt 0 00125 minutes lt 0 031 seconds 54 96 Hz G1314C 0 00125 minutes 0 031 seconds 27 48 Hz G1314C 0 0025 minutes 0 062 seconds 13 74 Hz G1314C 0 005 minutes 0 125 seconds 13 74 Hz G1314B G1314C 0 01 minutes 0 25 seconds 13 74 Hz G1314B G1314C 0 025 minutes 0 50 secon
94. eries Variable Wavelength Detector Service M anual 23 1 24 Introduction to the Variable Wavelength Detector 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 2 Site Requirements and Specifications Site Requirements 26 Physical Specifications 28 Performance Specifications 29 This chapter gives information on environmental requirements physical and performance specifications ee Agilent Technologies 25 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the detector Power Consideration The detector power supply has wide ranging capabilities see Table 2 on page 28 It accepts any line voltage in the above mentioned range Consequently there is no voltage selector in the rear of the detector There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply To disconnect the detector from line unplug the power cord The power supply still uses some power even if the power switch ON the front panel is turned OFF Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified Make sure to have easy access to the power cable of the instrument in order to disconnect the instrument from line Power Cords Different power cords are offered
95. etector s Firmware 151 Tests amp Calibrations 152 Intensity Test 153 1200 Series Variable Wavelength Detector Service M anual Wavelength Verification Calibration 155 Holmium Oxide Test 156 12 Repair Cautions and Warnings 161 Removing the Top Cover and Top Foams 163 Exchanging the Main Board 166 Changing the Type and Serial Number 169 Using the Agilent ChemStation 169 Using the Instant Pilot G2408A 170 Using the Control Module G1323B 171 Exchanging the Fan 173 Repairs in the Optical Unit 175 Special Notes 176 Contaminated Mirrors and Grating 177 Repairs inthe TopSection 178 Exchanging the Source Lens Assembly 179 Exchanging the Filter Assembly 182 Installing the Test Slit 185 Exchanging the Mirror 1 or 2 Assembly 187 Exchanging the Grating Assembly 190 Replacing the Beam Splitter 193 Optimizing The Sample Readings 196 Unlocking the Reference Aperture 197 Optimizing the Reference Readings 198 Installing the Standard Slit 199 Repairs inthe Bottom Section 201 Removing the Optical Unit 202 Exchanging a Photo Diode and or ADC Board Assembly 204 Replacing the Grating Motor or Position Sensor 208 Replacing the Filter Motor or Position Sensor 212 1200 Series Variable Wavelength Detector Service M anual Exchanging the Leak Sensor 216 Exchanging the Power Supply 218 Replacing Status Light Pipe 222 Installing the Optical Unit 223 Installing the Foam andthe Top Cover 225 Assembling the Main Cover 227 13 Parts and Materials
96. eviation from wavelength 2 418 Deviation from wavelength 3 536 4 nm Figure 46 Holmium Test Report Holmium Oxide Test Failed Probable Causes e Detector not calibrated Dirty or defective flow cell Dirty or defective holmium oxide filter e Optical misalignment 1200 Series Variable Wavelength Detector Service M anual 1 1 1 1 l l nm nm nm Measured Result 0 0 nm Passed 0 1 nm Passed 0 0 nm Passed 11 Maintenance Suggested Actions Y Re calibrate the detector V Repeat the test with the flow cell removed If the test is OK exchange the flow cell components V Run the holmium oxide filter test If the test fails exchange the filter assembly Y Realign the optical components 158 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 12 Repair Cautions and Warnings 161 Removing the Top Cover and Top Foams 163 Exchanging the Main Board 166 Changing the Type and Serial Number 169 Using the Agilent ChemStation 169 Using the Instant Pilot G2408A 170 Using the Control Module G1323B 171 Exchanging the Fan 173 Repairs in the Optical Unit 175 Contaminated Mirrors and Grating 177 Repairs inthe Top Section 178 Exchanging the Source Lens Assembly 179 Exchanging the Filter Assembly 182 Installing the Test Slit 185 Exchanging the Mirror 1 or 2 Assembly 187 Exchanging the Grating Assembly 190 Replacing the Beam Splitt
97. exchanging the leak sensor 216 for parts see parts for repair 239 inside optical unit 175 installing foams and top cover 225 installing optical unit 223 installing the standard slit 199 installing the test slit 185 introduction 129 Index of internal parts 130 optical unit bottom section 201 optical unit top section 178 optimizing sample readings 196 optimizing the reference readings 198 overview of simple repairs 136 removing optical unit 202 removing the optical unit 202 removing top cover and foam 163 replacing filter motor or position sensor 212 replacing grating motor or position sensor 208 replacing the beam splitter 193 simple maintenance 130 simple repairs see maintenance 135 unlocking the reference aperture 197 using the ESD strap 133 replacing firmware 151 parts see repairs 159 response time 66 73 RS 232C cable parts 273 connection table 293 interface 293 settings 297 running the sample 58 S safety class instrument 304 general information 304 lithium batteries 307 Switch 283 symbols 306 sample info 57 scan range step 64 scanning 61 serial number type entered on ChemStation 169 entered on control module 171 entered on instant pilot 170 service dialog 124 setting up an analysis 44 settings analog output settings 63 peakwidth 65 setup of detector 53 sheet metal kit parts 251 signal plot 55 polarity 64 sign
98. filtered dark current noise contribution of the front end electronics When the test is run the signal is output to the detector analog output When to do the Test e if the detector signal is noisy How to do it with the Agilent ChemStation On the Agilent ChemStation the test is evaluated automatically and checked against the limits Figure 33 Dark Current Test Report How to do it with the Control M odule G1323B On the Control Module the dark current counts are displayed as follows 1 Enable the Dark Current Test 2 Activate the Service Dialog m key Service 3 Select Signal 1 Raw Sample 4 Select Signal 2 Raw Reference 1200 Series Variable Wavelength Detector Service M anual Test Functions 8 5 Observe the count readings for signal 1 and signal 2 The displayed values should be less than 7900 counts The dark current signal can be displayed directly on the screen 1 Select in VWD Settings Analog Output as Raw Sample or Raw Reference 2 Press Plot and select the Analog signal in Setup 3 Return to Plot screen by pressing Done Interpreting the Results The output signal of the ADC noise corresponds to AD counts with 1 V or 0 1 V full scale setting The ADC noise must be taken over a time of 10 minutes for the results to be accepted Average counts should be less than 7900 counts Dark Current Test Failed Probable Causes e Defective sample or reference diode e Defective sample or reference VWA boar
99. for Maintenance Overview of Maintenance Parts 230 Standard Flow Cell 231 Micro Flow Cell 232 Semi micro Flow Cell 233 High Pressure Flow Cell 235 Cuvette Holder 236 Leak Parts 237 Accessory Kit 238 14 Parts for Repairs Overview of Main Assemblies 240 Optical Unit and Fan Assembly 242 Optical Unit Assembly 243 Source Lens and Filter Assembly 245 Grating Assembly 246 Filter Assembly 248 Lens Assembly 250 Sheet M etal Kit 251 Plastic Parts 252 1200 Series Variable Wavelength Detector Service M anual Leak Parts 253 Foam Parts 254 Power and Status Light Pipes 255 15 Identifying Cables Cable Overview 258 Analog Cables 260 Remote Cables 263 BCD Cables 268 Auxiliary Cable 270 CAN Cable 271 External Contact Cable 272 RS 232 Cable Kit 273 LAN Cables 274 16 Hardware Information Optical System Components 276 Detector Main Board VWM 280 Main features 280 Firmware 281 Leak Converter 281 Fan Drive 281 Filter Motor Driver 281 Grating Motor Driver 281 On board Battery 282 Interfaces 282 Deuterium Lamp Filament Control 282 Deuterium Lamp Current Control 282 Igniting the Deuterium Lamp 282 Diagnostic A D Converter 283 Safety Switch 283 Board Layout and Connectors 284 1200 Series Variable Wavelength Detector Service M anual 11 12 A Appendix Index Firmware Description 285 Firmware Updates 286 Optional Interface Boards 287 BCD Board 287 LAN Communication Interface Board 289 Agilent 1200 Series Interfa
100. formation on how to set up the detector for an analysis and explains the basic settings ee Agilent Technologies 43 4 Using the Detector Setting up an Analysis This chapter can be used for e preparing the system e to learn the set up of an HPLC analysis and e to use it as an instrument check to demonstrate that all modules of the system are correctly installed and connected It is not a test of the instrument performance e Learn about special settings Before Using the System Solvent Information Observe recommendations on the use of solvents in chapter Solvents in the pump s reference manual Priming and Purging the System When the solvents have been exchanged or the pumping system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel between the solvent reservoir vacuum degasser when available in the system and the pump Solvents containing volatile ingredients will slightly lose these Therefore priming of the pumping system is required before starting an application 44 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 Table 6 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system When switching between reverse Isopropanol phase and normal phase both times Best solvent to flush air out of the system After an in
101. fully supported by the software including module tests and calibrations as well as injector steps and maintenance positions e Agilent 1200 Series binary pump SL G1312B e Agilent 1200 Series high performance autosampler SL G1367B e Agilent 1200 Series thermostatted column compartment SL G1316B e Agilent 1200 Series diode array detector SL G1315C With further releases of the diagnostic software all Agilent 1200 Series HPLC modules will be fully supported This diagnostic software provides tests and diagnostic features that may differ from the descriptions in this manual For details refer to the help files provided with the diagnostic software 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual ee 7 A Error Information What Are Error Messages 82 General Error Messages 83 Detector Error Messages 94 This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from error conditions a Agilent Technologies i 7 Error Information What Are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indica
102. g Position Sensor G1314 65005 Preparation for this procedure are 1 Locate the grating unscrew and remove it Place it safe Install the Test Slit see Installing the Test Slit on page 185 Remove the optical unit from the instrument see Removing the Optical Unit on page 202 The top cover of the optical unit is removed 208 1200 Series Variable Wavelength Detector Service M anual Repair 12 2 Place the optical unit in normal position and remove the 3 Place the optical unit on its top and remove the cover to grating see Exchanging the Grating Assembly on have access to the bottom parts page 190 1200 Series Variable Wavelength Detector Service M anual 209 12 Repair 6 To replace the sensor unscrew it and replace it by the new one 7 To replace the motor remove the cover and unscrew the heat exchanger position sensor grating motor assembly sealing eat exchanger assembly cover 8 Replace the heat exchanger on the new motor assembly Then replace the cover 9 Assure that all three spacers are in place and replace the complete motor ass
103. h UV detector Optimization of the System The settings used for this analysis are specific for this purpose For other applications the system can be optimized in various ways Please refer to the section Optimizing the Detector on page 67 or Optimizing in the your module s Reference Manual 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 Preparing the HPLC System 1 Turn on the Agilent ChemStation PC and the monitor 2 Turn on the 1200 series HPLC modules 3 Start the Agilent ChemStation software B 02 01 If the pump autosampler thermostatted column compartment and detector are found the ChemStation screen should look like shown in Figure 10 The System status is red Not Ready System status Latte o wren Bag oer e s EME axa No ATE O E eT DEFAULT D ro Dotai d oseeeee2sss C eeta 1 1 GB bee a On line plot window details window Figure 10 Initial ChemStation screen M ethod and Run Control 1200 Series Variable Wavelength Detector Service M anual 49 4 Using the Detector 4 Turn on the detector lamp pump and autosampler by clicking the System On button or the buttons below the module icons on the graphical user interface GUI After some time the pump thermostatted column compartment and detector module will turn to green ER eee sure Si aD 2 DEFALAT D no Detal z ott ee sess B gt
104. he detector Unclip and remove the waste funnel assembly Disconnect the power cable Disconnect capillaries Remove detector from stack and place it on the working bench 1200 Series Variable Wavelength Detector Service M anual 163 12 Repair 2 Install the ESD strap M ove the power lock across the 3 If installed unscrew and remove the interface board power inlet Place the board on the ESD kit ESD strap NUL WW i 5 Unscrew the screws at the rear of the top plate slide the plate towards the front and remove it 164 1200 Series Variable Wavelength Detector Service M anual Repair 12 6 Carefully remove the top foams from the detector first Note pate Mel Rorke Do not connect a power plug to module after removing the top covers A safety light switch on the main board will turn off fan immediately and electronic after 30 seconds to avoid the operation with removed covers An error will be generated status lamp lights red and the logbook will show an error message The internal components are now accessible Exchanging specific components are described in the following sections 1200 Series Variable Wavelength Detector Service M anual 165 12 Repair Exchanging the M ain Board nuts Remote GPIB G1314B 166 When required If the detector main board is defective or for repair on other assemblies Tools req
105. hole in the optical unit cover Figure 50 Aligning Mirror 2 6 Optimize the reference readings with the reference aperture Optimizing the Reference Readings on page 198 7 Install the standard slit and perform electronic calibrations Installing the Standard Slit on page 199 196 1200 Series Variable Wavelength Detector Service M anual Repair 12 Unlocking the Reference Aperture Only necessary if required during a replacement procedure For performance reasons the reference aperture is fixed by one screw only and has to be unlocked prior to any replacement calibration within the optical unit if appropriate 1 Use a 2 5 mm hexagonal wrench to loosen the screw 2 Replace the cover of the optical unit 3 Turn on the detector and the lamp 4 Set the wavelength to 254 nm 5 Return to the replacement procedure Screw Figure 51 Unlocking the Reference Aperture 1200 Series Variable Wavelength Detector Service M anual 197 12 Repair Optimizing the Reference Readings a ur WN Install the test slit see Installing the Test Slit on page 185 Replace the cover of the optical unit Enter the Service Dialog see Service Dialog on page 124 Select Raw sample signal for value 1 and Raw reference signal for value 2 Press Zero order Shift reference aperture vertically for maximum reference readings Figure 52 Optimizing the Reference Read
106. ight beam into all its component wavelengths and reflects the light onto mirror 2 The grating has 1200 lines mm and is directly rotated by the grating drive stepper motor depending on the wavelength entry The whole range 190 to 600 nm is equal to 1550 steps 15 5 The stepper motor is controlled and driven by the detector main board VWM The step angle of the stepper motor rotation is electronically reduced by 0 01 per step This system has no mechanical reduction mechanism accordingly the grating is mounted directly onto the motor shaft The stepper motor reference position is determined by a plate fitted onto the motor shaft interrupting the beam of a photo sensor The wavelength calibration of the grating is done at the zero order light position and at 656 nm which is the emission line of the deuterium lamp The grating surface should not be touched or cleaned This will destroy the surface and reduce the light reflection Beam Splitter Assembly The beam splitter splits the light beam One part goes directly to the sample diode The other part of the light beam goes to the reference diode Photo Diodes Assemblies Two photo diode assemblies are installed in the optical unit The sample diode assembly is located on the left side of the optical unit The reference diode assembly is located in the front of the optical unit Photo Diode ADC analog to digital converter The photo diode current is directly converted to
107. in a low pressure deuterium gas the lamp emits light over the 190 to 600 nm wavelength range Source Lens Assembly The source lens receives the light from the deuterium lamp and focuses it onto the entrance slit 276 1200 Series Variable Wavelength Detector Service M anual Hardware Information 16 Entrance Slit Assembly The entrance slit assembly has an exchangeable slit The standard one has a 1 mm slit For replacement and calibration purposes to optimize the alignment a slit with a hole is needed Filter Assembly The filter assembly is electromechanically actuated During wavelength calibrations it moves into the light path The filter assembly has two filters installed and is processor controlled OPEN nothing in light path CUTOFF cut off filter in light path at gt 370 nm HOLMIUM holmium oxide filter for wavelength check A photo sensor determines the correct position Cutoff filter Holmium oxide filter with cutoff filter Figure 73 Filter Assembly Mirror Assemblies M 1 and M2 The instrument contains two spherical mirrors M1 and M2 The beam adjustable is vertically and horizontally Both mirrors are identical The mirrors are coated with magnesium fluoride They should not be touched or cleaned This will destroy the surface and reduce the light reflection 1200 Series Variable Wavelength Detector Service M anual 277 16 Hardware Information 278 Grating Assembly The grating separates the l
108. in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid e Mixtures of carbon tetrachloride with 2 propanol or THF 312 1200 Series Variable Wavelength Detector Service M anual Appendix A Declaration of Conformity for HOX2 Filter Declaration of Conformity We herevrith infomm you that the Holmium Oxide Glass Filter Type Hoya HY 1 Part No 79380 22711 meets the followring specification of absorbance maxima positions mema waga ccc pasa Accura aan La lym i C 79853 1050 gam Srm ee ren 418 Sr gam dron G13144 B C 1100 1200 360 8ren aa lrm 418 5 536 4ron The variation in Measured Wavelength depends on the different Optical Bandwidth Agilent Technologies guarantees the trace ability of the specified absorbance maxima to a National Instinate of Standards amp Technology NIST Holnimm Oxide Sobution Qandard with a lot to lot tolerance of 0 3 rm The wavelength calibration filter built into the Agilent Technologies UV VIS detectors is made of this material and meets these specifications E is therefore suitable for wavelength calibration of these detectors vrithin the specified wavelength accuracy of the respective dete ctor over its vravelength rimge Juary 13 2006 Date P N 89550 90501 Revision E abe Agilent Techaologies TT une 1200 Series Variable Wavelength Detector Service M anual 313 A Ap
109. indow and the gasket CAUTION Do not use tweezers to remove windows as the surfaces can easily be scratched 4 Repeat step 1 through step 3 for the other window keep the parts separate otherwise they could be mixed Cleaning the Flow 5 Pour isopropanol into the cell hole and wipe clean with a piece of lint free Cell Parts cloth 6 Clean the windows with ethanol or methanol Dry it with a piece of lint free cloth Always use new gaskets Reassembling the 7 Hold the flow cell cassette horizontally and place gasket in position Flow Cell Ensure both cell holes can be seen through the holes of gasket The semi micro 1 and 2 gaskets items 6 and 7 Semi micro Flow Cell on page 234 look very similar Do not mix them up 8 Place the window on gasket 9 Place the peek ring on the window 10 Insert the conical springs Make sure the conical springs point towards the window Otherwise tightening the cell screw might break the window 11 Screw the cell screw into the flow cell and tighten the screw 12 Repeat the procedure for the other cell side 1200 Series Variable Wavelength Detector Service M anual 143 11 Maintenance 144 Next steps Reconnect the capillaries see Exchanging a Flow Cell on page 139 Perform a leak test If OK insert the flow cell Perform Wavelength Verification Calibration on page 155 to check the correct positioning of the flow cell Replace the front cover 1200 Series Variable
110. ings 198 Remove the top cover without touching the reference handle Fix the reference slit with the top lock screw of the photo diode assembly Figure 4 Continue with the next step of the procedure of the assembly you are replacing 1200 Series Variable Wavelength Detector Service M anual Repair 12 Installing the Standard Slit When required This procedure has to be carried out at the beginning of all replacement procedures Tools required Screwdriver POZI 1 PT3 Wrench 1 4 1 5 inch A pointed pair of pliers Parts required Standard Slit part of Slit Kit 79853 68746 1 Carefully remove the cover of the optical unit Take care for 2 Remove the test slit from the entrance slit holder using a the reference aperture handle pair of tweezers and place it safe 1200 Series Variable Wavelength Detector Service M anual 199 12 Repair 3 Carefully insert the standard slit into the entrance slit 4 Replace the cover of the optical unit holder starting at the arrow side first The slit must sit plane on the holder 5 Execute Wavelength Calibration to calibrate the grating 6 Fix the screws of the optical unit s cover 7 Turn the instrument OFF 8 Reinstall the front cover top cover and top foam section 9 Replace module into the stack 10 Reconnect the cables and capillaries and turn ON the module 200 1200 Series Variable Wavelength Detector Service M anual Repair 12
111. intenance Overview of Maintenance Parts 230 Standard Flow Cell 231 Micro Flow Cell 232 Semi micro Flow Cell 233 High Pressure Flow Cell 235 Cuvette Holder 236 Leak Parts 237 Accessory Kit 238 This chapter provides information on parts for maintenance ott Agilent Technologies 229 13 Parts and Materials for Maintenance Overview of Maintenance Parts Table 19 Maintenance Parts Item Description Part Number Cable CAN assembly 0 5 m 5181 1516 Cable CAN assembly 1 m 5181 1519 Interface board BCD External Contacts G1351 68701 LAN Communication Interface board G1369A or G1369 60001 Control M odule G1323B Note The G1314C VW D SL can be operated with a G1323B just G1323 67001 in standard mode as G1314B no higher data rate available or Instant Pilot G4208A 64208 67001 Deuterium lamp G1314 60100 Standard flow cell 10 mm 14 ul additional flow cell parts see page 231 G1314 60086 Micro flow cell 5mm 1 ul additional flow cell parts see page 232 G1314 60081 High pressure flow cell 10 mm 14 ul additional flow cell parts see page 235 G1314 60082 Semi micro flow cell 6 mm 5 ul additional flow cell parts see page 233 G1314 60083 Cuvette Holder G1314 60200 Front cover 5065 9982 Leak handling parts see page 237 230 1200 Series Variable Wavelength Detector Service M anual Parts and Materials for Maintenance 13 Standard Flow Cell Table 20 Standard Flow Cell Assembly Item Description Part Numbe
112. ion from wavelength 1 360 8 nm 1 1 nm 0 0 nm Passed Deviation from wavelength 2 418 5 nm 1 1 nm 0 1 nm Passed Deviation from wavelength 3 536 4 nm 1 1 nm 0 0 nm Passed Figure 31 Holmium Test Report Holmium Oxide Test Failed Probable Causes e Detector not calibrated Dirty or defective flow cell Dirty or defective holmium oxide filter e Optical misalignment 110 1200 Series Variable W avelength Detector Service M anual Test Functions Suggested Actions Y Recalibrate the detector V Repeat the test with the flow cell removed If the test is OK exchange the flow cell components V Run the holmium oxide filter test If the test fails exchange the filter assembly Y Realign the optical components 1200 Series Variable Wavelength Detector Service M anual 8 111 8 112 Test Functions Intensity Test The intensity test measures the intensity of the deuterium lamp over the full VWD wavelength range 190 600 nm The test can be used to determine the performance of the lamp and to check for dirty or contaminated flow cell windows When the test is started the gain is set to zero To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The shape of the intensity spectrum is primarily dependent on the lamp grating and diode characteristics Therefore intensity spectra will differ slightly between instruments Figure 32 shows a typical intensity test s
113. ir bubbles 1200 Series Variable Wavelength Detector Service M anual Test Functions 8 Holmium Oxide Test This test verifies the calibration of the detector against the three wavelength maxima of the built in holmium oxide filter The test displays the difference between the expected and measured maxima Figure 31 shows a holmium test spectrum The test uses the following holmium maxima 360 8 nm e 418 5 nm e 536 4 nm See also Declaration of Conformity for HOX2 Filter on page 313 When to do the Test e after recalibration e as part of the Operational Qualification Performance Verification procedure or e after flow cell maintenance or repair Interpreting the Results The test is passed successfully when all three wavelengths are within 1 nm of the expected value This indicates the detector is calibrated correctly The test results are currently available on the Agilent ChemStation only ChemStation revisions below B 01 xx show a limit of 2 nm It should read 1 nm If the test shows a value greater than 1 nm perform a recalibration 1200 Series Variable Wavelength Detector Service M anual 109 8 Test Functions Ins G1314B Ser JP33324886 Ope Wolfgang Dat 03 01 2006 Tim 15 26 41 Fil C CHEM32 2 DIAGNOSE VWD_HOLM DGR N 1 i j 1 1 OS Ones 0 7 oe S ny 200 300 400 500 600 Wavelength nm VWD Holmium Test Results Specification Measured Result Deviat
114. ischarged Lithium batteries through carriers regulated by IATA ICAO ADR RID IM DG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries Lithium batteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1200 Series Variable Wavelength Detector Service M anual 307 A Appendix Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 308 1200 Series Variable Wavelength Detector Service M anual Appendix A Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emi
115. l ground NC 2 Brown Prepare run Low 7 3 Gray Start Low D NC 4 Blue Shut down Low NC 5 Pink Not connected Q NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start request Low 1200 Series Variable Wavelength Detector Service M anual 263 15 Identifying Cables Agilent 1200 to 3392 3 Integrators Connector Pin Pin SignalName Active 01046 60206 3392 3 Agilent 1200 TTL 3 1 White Digital ground NC 2 Brown Prepare run Low ON 11 3 Gray Start Low Q OO NC 4 Blue Shut down Low K NC 5 Pink Not connected NE NC 6 Yellow Poweron High 9 7 Red Ready High 4 Key 1 8 Green Stop Low NC 9 Black Start request Low Agilent 1200 to 3394 Integrators Connector Pin Pin Signal Name Active 01046 60210 3394 Agilent 1200 TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low P 15 3 3 Gray Start Low a NC 4 Blue Shut down Low o NC 5 Pink Not connected ie NC 6 Yellow Power on High ao 5 14 7 Red Ready High 6 8 Green Stop Low 1 9 Black Start request Low 13 15 Not connected 264 1200 Series Variable Wavelength Detector Service M anual Identifying Cables 15 START and STOP are connected via diodes to pin 3 of the 3394 connector Agilent 1200 to 3396A Integrators Connector Pin Pin SignalName Active 03394 60600 3394 Agilent 120
116. lar interval at least once per year for example prior to an Operational Qualification Performance Verification procedure and e when chromatographic results indicate the detector may require recalibration Zero Order Calibration The zero order calibration function is used for the electrical calibration of the zero order beam of the monochromator The grating is driven by the stepper motor while the detector searches for the intensity maximum occurring at the zero order position The zero order calibration values are stored in the instrument The deviation is displayed in nm on the user interface see Figure 29 on page 105 656 nm Wavelength Calibration The wavelength calibration function is used to calibrate the monochrometer against the 656 nm emission line of the deuterium lamp The detector searches for maximum light intensity in the region of the 656 nm emission line The 656 nm calibration values are stored in the instrument The deviation is displayed in nm on the user interface see Figure 29 on page 105 1200 Series Variable W avelength Detector Service M anual Test Functions 8 Lamp on Routine When the lamp is turned on the following routine is implemented During the ignition phase the grating position is reinitialize by moving the grating position lever into the sensor position After ignition a 1 minute warm up phase should stabilize the lamp Check of wavelength setting at 656 nm emission line must b
117. lation of flow connections 40 of module 37 instant pilot 79 instrument layout 21 interface Agilent 1200 Series 290 analog signal output 291 BCD 287 CAN 291 GPIB 291 remote 292 RS 232C 293 internet 314 introduction 14 to optical system 15 276 isocratic test sample 58 L lamp 17 276 current control 282 filament control 282 ignition 282 initial drift 56 intensity test 112 153 turn on routine 107 type 29 59 LAN cables parts 274 communication interface board 289 layout of main board 284 leak converter 281 correcting 148 panels parts 237 253 line power consumption 28 voltage and frequency 28 linearity 29 30 M maintenance 135 cleaning the instrument 132 correcting leaks 148 exchanging flow cell 139 exchanging interface board 150 exchanging lamps 137 exchanging leak handling system 149 for parts see parts for maintenance 229 introduction 129 overview 136 replacing firmware 151 simple repairs 130 standard flow cell 142 using the cuvette holder 145 using the ESD strap 133 message calibration failed 98 calibration lost 101 compensation sensor open 90 compensation sensor short 91 fan failed 92 grating filter motor defective 100 heater current missing 97 holmium oxide test failed 99 ignition without cover 93 lamp current missing 94 lamp ignition failed 96 lamp voltage missing 95 leak 87 leak sensor open 88 leak sensor short 89 me
118. les TYPE G1314B to configure as a VWD TYPE G1314C to configure as a VWD SL To configure the detector as G1314C VW D SL requires the correct main board version If you enter the wrong type your module might not be accessible anymore with the Agilent ChemS tation In such a case re enter the TYPE command correctly 172 Now press the Execute key Below the box a reply line should then say Reply RA 0000 TYPE XXXXX XXXXX is what you just entered Turn the detector off then on again Turn on should be normal In the Records screen the product column should indicate the module you just entered If an Agilent ChemStation is also connected re boot it now 1200 Series Variable Wavelength Detector Service M anual Repair 12 Exchanging the Fan When required If the fan is defective or noisy Tools required Screwdriver POZI 1 PT3 Parts required Fan G1314 65004 Other parts see Optical Unit and Fan Assembly on page 242 CAUTION The fan must be installed in the correct orientation to ensure optimum cooling and operation of the detector With the introduction of the 1200 Series VW D the fan protection cover grille was changed slightly For parts see Optical Unit and Fan Assembly on page 242 It has to Preparations for this procedure l Disconnect the fan from the main board and remove the Turn OFF the lamp fellespembly Switch OFF the detector and disconnect the cables Remove detector from stack and place i
119. ll Troubleshooting and Diagnostics This chapter describes the detector s built in test functions Error Information This chapter describes the meaning of detector error messages and provides information on probable causes and suggested actions how to recover from error conditions 1200 Series Variable Wavelength Detector Service Manual 3 10 11 12 13 14 15 16 Test Functions This chapter describes the detector s built in test functions Diagnosis Signals This chapter describes the detector s built in diagnostics Maintenance and Repair This chapter provides general information on maintenance and repair of the detector Maintenance This chapter describes the maintenance of the detector Repair This chapter gives instructions on how to repair the detector Parts and M aterials for M aintenance This chapter provides information on parts for maintenance Parts for Repairs This chapter provides information on parts for repair Identifying Cables This chapter provides information on cables used with the 1200 series of HPLC modules Hardware Information This chapter describes the detector in more detail on hardware and electronics Appendix This chapter provides addition information on safety legal and web 1200 Series Variable Wavelength Detector Service M anual Contents 1 Introduction to the Variable Wavelength Detector Introduction to the Detector 14 Optical System Overview 1
120. lter Check Failed The automatic filter check after lamp ignition has failed When the lamp is switched on the detector moves the cutoff filter into the light path If the filter is functioning correctly a decrease in lamp intensity is seen If the expected intensity decrease is not detected the error message is generated Probable Causes e Filter motor defective e Defective or missing filter Suggested Actions Y Run the filter motor test to determine if the motor is defective V Exchange the filter assembly 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual Pa ee 8 e Test Functions J Available Tests versus Interfaces 104 Wavelength Verification Calibration 105 When to Calibrate the Detector 106 Zero Order Calibration 106 656 nm Wavelength Calibration 106 Lamp on Routine 107 Checking the Photocurrent 108 Holmium Oxide Test 109 Intensity Test 112 Dark Current Test 114 DAC Test 116 Grating Motor Test 117 Filter M otor Test 118 Test Chromatogram 119 This chapter describes the detector s built in test functions Agg Agilent Technologies 103 8 Test Functions Available Tests versus Interfaces Depending on the user interface the available tests vary All test descriptions are based on the Agilent ChemStation as user interface Table 14 Available Tests versus Interface Interface ChemStation Test Instant Pilot
121. ly 248 foams 254 grating assembly 246 leak panels 253 lens assembly parts 250 main assemblies 240 optical unit and fan 242 plastics 252 power and status 255 sheet metal kit 251 source lens and filter source lens and filter parts 245 peakw idth settings 54 65 73 performance optimization 70 specifications 29 photodiode assemblies 18 278 boards 18 278 readings 108 photometric accuracy 72 physical specifications 28 humidity 28 line voltage and frequency 28 1200 Series Variable Wavelength Detector Service M anual Index operating temperature 28 power consumption 28 safety information 28 weight and dimensions 28 plastics parts 252 power considerations 26 consumption 28 cords 26 power supply description 300 parts 255 preparing the HPLC system 49 R radio interference 308 rear of detector 38 recalibration of wavelength 76 reference conditions 30 remote cables parts 263 interface 292 signal distribution 292 repairs 159 assembling main cover 227 cautions and warnings 161 cleaning the instrument 132 exchanging fan 173 exchanging holmium filter 182 exchanging leak sensor 216 exchanging mirror 1 or 2 assembly 187 exchanging photodiode and or ADC board 204 exchanging power supply 218 exchanging processor board 166 exchanging source lens 179 exchanging status light pipe 222 exchanging the filter assembly 182 exchanging the grating assembly 190
122. m is primarily dependent on the lamp grating and diode characteristics Therefore intensity spectra will differ slightly between instruments Figure 44 shows a typical intensity test spectrum Intensity Test Evaluation Agilent ChemStation only The Agilent ChemStation evaluates three values automatically and displays the limits for each value the average the minimum and the maximum of all data points and passed or failed for each value Test Failed Probable Causes e Absorbing solvent in flow cell e Dirty or contaminated flow cell e Dirty or contaminated optical components source lens mirrors grating e Old or non Agilent lamp Suggested Actions Y Ensure the flow cell is filled with water V Repeat the test with the flow cell removed If the test passes exchange the flow cell windows Y Clean replace optical components V Exchange the lamp 1200 Series Variable Wavelength Detector Service M anual 153 11 Maintenance 154 Instrument G1314B Serial Number JP33324886 Operator Wolfgang Date 03 01 2006 Time 15 07 09 File C CHEM32 2 DIAGNOSE VWD_INT DGR VWD Intensity Spectrum Accumulated lamp on time 94 35 h Highest intensity gt 10000 cts 222615 cts Average intensity gt 000 cts 29734 cts Lowest intensity gt 200 cts 1137 cts Figure 44 Intensity Test Report Passed Passed Passed 1200 Series Variable Wavelength Detector Service M anual Maintenance 11 Wavelength Verific
123. m long 5023 0203 for point to point connection Twisted pair network cable shielded 7 m long 5023 0202 for hub connections 274 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 16 Hardware Information Optical System Components 276 Detector Main Board VWM 280 Firmware Description 285 Optional Interface Boards 287 Agilent 1200 Series Interfaces 290 Setting the 8 bit Configuration Switch 295 Main Power Supply Assembly 300 This chapter describes the detector in more detail on hardware and electronics a Agilent Technologies 275 16 Hardware Information Optical System Components Grating assembly Entrance slit assembly Beam splitter assembly Mirror 1 Filter assembly Sourcelens _ l assembly 1 Lamp Mirror 2 Photo diode and ADC SAMP ADC REF Photo diode REF Flow cell assembly Figure 72 Mechanical Layout of the Optical Unit Lamp The light source for the UV wavelength range is a deuterium lamp As a result of plasma discharge
124. merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Restricted Rights Legend Software and technical data rights granted to federal government customers include only those rights customarily provided to end user Customers of Software Agilent provides this customary commercial license in Software and technical data pursuant to FAR 12 211 Technical Data and FAR 12 212 Computer Software and for Department of Defense purchases DFARS 252 227 7015 Technical Data Commercial Items and DFARS 227 7202 3 Rights in Commercial Computer Software or Computer Software Documentation Ifa federal government or other public sector Customer has a need for rights not conveyed under these terms it must negotiate with Agilent to establish acceptable terms in a written agreement executed by all relevant parties Safety Notices CAUTION A CAUT
125. motor driver controlled by the main processor drives the grating motor The motor driver controls and balances each coil current of the 2 phase stepper motor corrected micro step stepper motor driver circuit The step angle of the stepper motor rotation is reduced electronically into 0 01 per step The data of current control for detent torque correction is programmed on ROM Read Only Memory 1200 Series Variable Wavelength Detector Service M anual 281 16 Hardware Information 282 On board Battery An on board lithium battery buffers the electronic memories when the module is turned OFF For safety information on lithium batteries Lithium Batteries Information on page 307 Interfaces For detailed information on interfaces see Agilent 1200 Series Interfaces on page 290 Deuterium Lamp Filament Control The deuterium lamp filament control circuit provides a constant voltage of 2 5 VDC at approximately 6 A to the filament of the deuterium lamp The deuterium lamp filament control circuit is enabled by the processor Depending on the lamp type the heater voltage is turned off or kept lower after lamp ignition Deuterium Lamp Current Control The deuterium lamp current control circuit comprises two parts One part generates an ignition pulse of 600 VDC for the lamp resulting in lamp ignition After ignition this voltage is disabled The other part is a constant current source of 320 mA at an operating voltage of abou
126. mp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically 1200 Series Variable Wavelength Detector Service M anual 155 11 Maintenance Holmium Oxide Test This test verifies the calibration of the detector against the three wavelength maxima of the built in holmium oxide filter The test displays the difference between the expected and measured maxima Figure 46 shows a holmium test spectrum The test uses the following holmium maxima e 360 8 nm 418 5 nm e 536 4 nm See also Declaration of Conformity for HOX2 Filter on page 313 When to do the Test e after recalibration e as part of the Operational Qualification Performance Verification procedure or e after flow cell maintenance or repair Interpreting the Results The test is passed successfully when all three wavelengths are within 1 nm of the expected value This indicates the detector is calibrated correctly The test results are currently available on the Agilent ChemStation only ChemStation revisions below B 01 xx show a limit of 2 nm It should read 1 nm If the test shows a value greater than 1 nm perform a recalibration 156 1200 Series Variable Wavelength Detector Service M anual Ins G1314B Ser JP33324886 Ope Wolfgang Dat 03 01 2006 Tim 15 26 41 Fil C CHEM32 2 DIAGNOSE N VWD_HOLM DGR Maintenance 11 VWD Holmium Test Results sry Deviation from wavelength 1 D
127. nce 11 Exchanging a Flow Cell When required If application needs a different type of flow cell or the flow cell needs repair Tools required Two 1 4inch wrenches for capillary connections Parts required G1314 60086 10 mm 14 ul 40 bar Micro flow cell 5 mm 1 ul 40 bar G1314 60081 Semimicro flow cell 6mm 5 ul 40 bar G1314 60083 High pressure flow cell 10 mm 14 ul 400 bar G1314 60082 Preparations for this procedure l Press the release buttons and remove the front cover to have access to the flow cell area Turn the lamp OFF 1200 Series Variable Wavelength Detector Service M anual 139 11 Maintenance 2 Disconnect the inlet and outlet capillaries a TREAT gt 3 Unscrew the thumb screws parallel and remove the flow cell 7 so INNS TH gt we Note If you want to maintain flow cell parts see Repairing the Flow Cells on page 142 or the information provided with your flow cell 4 Replace the flow cell and fix the thumb screws Reconnect the inlet and outlet capillaries to the flow cell 140 1200 Series Variable W avelength Detector Service M anual Maintenance 11 5 Replace the front cover Next steps To check for leaks establish a flow and observe the flow cell outside of
128. nce image NA N Sy x ae N gt co d Cid Il 7 Replace the beam splitter assembly 8 Position horizontally the white image center of the beam Splitter onto the reference slit Fix the beam splitter after precise image fit 194 1200 Series Variable Wavelength Detector Service M anual Repair 12 9 Correct new vertical position with reference slit Replace the cover of the optical unit Perform a BALANCE Set 1 254 nm Enter the Service Dialog see Service Dialog on page 124 Select Raw sample signal for value 1 and Raw reference signal for value 2 Press Zero order Optimize the sample readings using the setscrew of mirror 2 through the hole in the optical unit cover see Optimizing The Sample Readings on page 196 Optimize the reference readings see Optimizing the Reference Readings on page 198 Install the standard slit and perform electronic calibrations see Installing the Standard Slit on page 199 1200 Series Variable Wavelength Detector Service M anual 195 12 Repair Optimizing The Sample Readings Install the test slit see Installing the Test Slit on page 185 Set 1 254 nm Enter the Service Dialog see Service Dialog on page 124 Select Raw sample signal for value 1 and Raw reference signal for value 2 ur WN Pe Optimize the sample readings using the setscrew of mirror 2 through the
129. necessary see also Set the Detector Parameters on page 73 1 To change the Peakwidth settings select Setup Detector Signals 2 In the section Peakwidth Responsetime click on the drop down list 3 Change the Peakwidth according to your needs Peakwidth enables you to select the peak width response time of Control for your analysis The peak width is defined as the width of a peak Eu Online Spectra in minutes at half the peak height Set the peak width to the narrowest expected peak in your chromatogram The peak width sets the optimum response time for your detector The peak 8 Not Ready Information Hel detector ignores any peaks that are considerably narrower or wider than the peak width setting The response time is the time Signal between 10 and 90 of the output signal in response to an input step function When the All spectrum storage option is selected Wavelength then spectra are acquired continuously depending on the setting 254 E of the peak width The time specified by the peak width is used as a factor in the acquisition of spectra The acquisition time for one spectrum is slightly less than the peak width divided by 8 see Table 9 on page 66 Peakwidth Responsetime gt 01 min 2s Limits W hen you set the peak width in minutes the corresponding response time is set automatically and the appropriate data rate for signal acquisition is selected as shown in Table 9 on page 66 gt
130. nes In addition PREPARE and START REQUEST may be issued The signal level are defined as e standard TTL levels 0 V is logic true 5 V is false e fan out is 10 e input load is 2 2 kOhm against 5 V and e output are open collector type inputs outputs wired or technique Table 47 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing preanalysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 1200 Series Variable Wavelength Detector Service M anual Hardware Information 16 Table 47 Remote Signal Distribution continued Pin Signal Description 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosam
131. ngaLamp 137 Exchanging a Flow Cell 139 Repairing the Flow Cells 142 Using the Cuvette Holder 145 Correcting Leaks 148 Replacing Leak Handling System Parts 149 Replacing the Interface Board 150 Replacing the Detector s Firmware 151 Tests amp Calibrations 152 Intensity Test 153 Wavelength Verification Calibration 155 Holmium Oxide Test 156 This chapter describes the maintenance of the detector ott Agilent Technologies 135 11 Maintenance Overview of Maintenance The following pages describe maintenance simple repairs of the detector that can be carried out without opening the main cover Table 18 Simple Repairs Procedures Typical Frequency Notes Deuterium lamp If noise and or drift exceeds your application limits or A VWD test should be performed after exchange lamp does not ignite replacement Flow cell exchange If application requires a different flow cell type A VWD test should be performed after replacement Cleaning flow cell parts If leaking or if intensity drops due to contaminated flow A pressure tightness test should be cleaning orexchange cell windows done after repair Leak sensor drying If leak has occurred Check for leaks Leak handling system If broken or corroded Check for leaks replacement 136 1200 Series Variable Wavelength Detector Service M anual Maintenance 11 Exchanging a Lamp When required If noise or drift exceeds application limits or lamp does not ignite Tools r
132. nject Sequence F 180 O1D li Prefix Counter z p QI DGCALAS M rm Sample Parameters Location vial 1 blank run if no entry Sample Name fisocratic test sample Sample Amount 0 Multiplier fi ISTD Amount fo Dilution fi Comment Isocratic test sample 1 ul 30 70 H2O Acetonitrile 1 5 ml min H Run Method OK Cancel Help Figure 18 Sample Information 15 Fill the content of an isocratic standard sample ampoule into a vial and seal the vial with a cap and place the vial into autosampler tray position 1 1200 Series Variable Wavelength Detector Service M anual 57 4 Using the Detector Running the Sample and Verifying the Results 1 To start a run select the menu item RunControl gt Run Method 2 This will start the 1200 modules and the online plot on the Agilent ChemStation will show the resulting chromatogram DADI A Sip 2544 Ref G00 190 0 1101 4 10 100003 D gt ran Figure 19 Chromatogram with Isocratic Test Sample Information about using the Data Analysis functions can be obtained from the Using your ChemStation manual supplied with your system 58 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 Special Settings of the Detector In this chapter special settings of the G1314B VWD and G1314C VWD SL are described based on the Agilent ChemStation B 02 01 Control Settings Set up VWD Signal x e Lamp turn
133. nto the stack and reconnect the cables and capillaries 4Turn ON the detector 222 1200 Series Variable Wavelength Detector Service M anual Repair 12 Installing the Optical Unit When required To continue the installation Tools required Screwdriver POZI 1 PT3 Wrench 1 4 inch Prerequisites The optical unit is already removed All other assemblies are already installed Preparation for this procedure are 1 Place the optical unit into the bottom foam The power supply the fan and the processor board are already installed 1200 Series Variable Wavelength Detector Service M anual 223 12 Repair 2 Reconnect all cables to the main board and the deuterium 3 Replace the lamp cover and the plate that secures the lamp connector optical unit 4 Replace the bumpers in the top foam so that they are Next steps ositioned on the optical unit as shown p p Reinstall the front cover top cover and top foam section see Installing the Foam and the Top Cover on page 225 e Replace the detector into the stack e Reconnect the cables and capillaries and turn ON the detector 224 1200 Series Variable Wavelength Detector Service M anual Repair 12 Installing the Foam and the Top Cover When required When all repairs have been completed Tools required Screwdriver POZI 1 PT3 Prerequisites The detector is open and other procedures have been carried ou
134. on and off of a Control Lamp p Error Method UV lamp fy Online Spectra RA E in T Take current method AtPower On automatic lamp on vot Reaay Information C off at power on Help S r Analog Output Range P Error Method take error method or current method in case of an error Cov 1 At Power On m Lamp Type Analog Output Range can be W pa 6131460100 set to either 100 mV or 1 V full scale see also Analog Output Settings on page 63 gt Automatic Turn On Moe Lamp Type can be set to either Date 29 12 2005 dd rim yyyy G1314 60100 standard VW D Tine PUEDE lt Phriomrrs lamp or 2140 0590 DAD lamps see also Exchanging a Lamp on page 137 Cereal e Automatic Turn On lamps can be programmed detector must be on for this Help online help Figure 20 Detector control settings 1200 Series Variable Wavelength Detector Service Manual 59 4 Using the Detector Online Spectra 1 To view the online spectra select Online Spectra NOTE This online spectrum is taken during a stop flow condition only while the peak is kept in the flow cell see Scanning with the VW D on page 61 2 Not Ready Information al a 100 005 1000 0 Figure 21 Online Spectra Window 2 Change the absorbance and wavelength range according your needs 60 1200 Series Variable Wavelength Detector Service M anual Using the Dete
135. ons Table3 Performance Specifications Agilent 1200 Series Variable Wavelength Detector continued Type Specification Comments Communications Controller area network CAN GPIB for G1314B only GPIB RS 232C APG Remote ready start stop and shut down signals LAN optional Safety and maintenance Extensive diagnostics error detection and display through control module and Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas GLP features Early maintenance feedback EM F for continuous tracking of instrument usage in terms of lamp burn time with user settable limits and feedback messages Electronic records of maintenance and errors Verification of wavelength accuracy with built in holmium oxide filter Housing All materials recyclable ASTM Standard Practice for Variable Wavelength Photometric Detectors Used in Liquid Chromatography Reference conditions cell path length 10 mm response time 2 s flow 1 ml min LC grade methanol Linearity measured with caffeine at 265 nm 30 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 3 Installing the Detector Unpacking the Detector 32 Optimizing the Stack Configuration 34 Installing the Detector 37 Flow Connections to the Detector 40 This chapter describes the installation of the
136. oring the instrument Safety standards IEC CSA UL EN Installation Category Il Pollution Degree 2 For indoor use only 28 1200 Series Variable Wavelength Detector Service M anual Site Requirements and Specifications 2 Performance Specifications Table3 Performance Specifications Agilent 1200 Series Variable Wavelength Detector Type Specification Comments Detection type Light source Wavelength range Short term noise ASTM Drift Linearity Wavelength accuracy Band width Flow cells Control and data evaluation Analog outputs Double beam photometer Deuterium lamp 190 600 nm 0 75 x 10 AU at 254 nm See NOTE on page 30 3 x 10 AU hr at 254 nm See NOTE on page 30 gt 2 AU 5 upper limit See NOTE on page 30 1nm Self calibration with deuterium lines verification with holmium oxide filter 6 5 nm typical Standard 14 ul volume 10 mm Can be repaired on component level cell path length and 40 bar 588 psi pressure maximum High pressure 14 1 volume 10 mm cell path length and 400 bar 5880 psi pressure maximum Micro 1 ul volume 5 mm cell path length and 40 bar 588 psi pressure maximum Semi micro 5 ul volume 6 mm cell path length and 40 bar 588 psi pressure maximum Agilent ChemStation for LC Recorder integrator 100 mV or 1V output range 0 001 2 AU one output 1200 Series Variable Wavelength Detector Service M anual 29 2 Site Requirements and Specificati
137. ove Main features e lamp supply for UV lamp e interface slot for LAN or BCD external contact board e CAN for internal 1200 interfacing e RS 232 for third part control or service e 1 analog output 0 1 V or 0 100 mV full scale e leak sensing flow cell e safety switch turns off high voltages fan battery for parameter backup 280 1200 Series Variable Wavelength Detector Service M anual Hardware Information 16 Firmware For an outline of the firmware see Firmware Description on page 285 Leak Converter This block consists of a PTC resistor with positive temperature coefficient for the leak identification and a NTC resistor with negative temperature coefficient for the ambient temperature measurement This assures that temperature changes are not identified as a leak A leak would cool down the PTC and its change in resistance would produce a leak signal The signals are converted by the diagnostic A D converter Fan Drive The operation of the fan is controlled by the main processor and runs with constant revolution The fan produces a sense signal which is derived from the revolution This sense signal is used for diagnostics If the fan does not turn the deuterium lamp and the grating drive are switched OFF Filter M otor Driver The filter motor driver controlled by the main processor drives the filter motor with constant voltage 2 phase full stepping Grating Motor Driver The grating
138. pectrum Intensity Test Evaluation Agilent ChemStation only The Agilent ChemStation evaluates three values automatically and displays the limits for each value the average the minimum and the maximum of all data points and passed or failed for each value Test Failed Probable Causes e Absorbing solvent in flow cell e Dirty or contaminated flow cell e Dirty or contaminated optical components source lens mirrors grating e Old or non Agilent lamp Suggested Actions Y Ensure the flow cell is filled with water V Repeat the test with the flow cell removed If the test passes exchange the flow cell windows V Clean replace optical components V Exchange the lamp 1200 Series Variable Wavelength Detector Service M anual Instrument G1314B Serial Number JP33324886 Operator Wolfgang Date 03 01 2006 Time 15 07 09 File C CHEM32 2 DIAGNOSE VWD_INT DGR VWD Intensity Spectrum Test Functions 8 Accumulated lamp n time Highest intensity gt 10000 cts Average intensity gt 5000 cts Lowest intensity gt 200 cts Figure 32 Intensity Test Report 1200 Series Variable Wavelength Detector Service M anual 94 35 h 222615 cts Passed 29734 cts Passed 1137 cts Passed 113 8 114 Test Functions Dark Current Test This test determines the dark current noise contribution of the front end electronics During the test the lamp is switched OFF The resulting signal corresponds to the un
139. pendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1200 Series modules for download 314 1200 Series Variable Wavelength Detector Service M anual Index A A D converter 283 absorbance Beer Lambert 71 accessory kit parts 238 address GPIB 291 296 Agilent ChemStation 49 LC diagnostic software 80 on internet 314 Agilent on internet 314 algea information 311 analog cables parts 260 output range 59 63 output settings 63 signal output 291 APG remote connector 292 ASTM environmental conditions 27 reference and conditions 30 attenuation 54 autobalance 54 automatic turn on 59 auxiliary cable parts 270 band width 6 5 nm 29 battery 282 safety information 307 BCD board description 287 cables parts 268 beam splitter 18 278 Beer Lambert law 71 bench space 27 board temperature 127 boards detector main board VWM 280 external contacts 287 HP J etDirect card 289 interface boards BCD 287 LAN communication interface 289 photodiode boards ADC 18 278 VWM layout and connectors 284 C cable connecting APG remote 36 connecting CAN 36 connecting GPIB 36 connecting LAN 36 connecting the analog 38 connecting the APG remote 38 connecting
140. pensation Sensor Short The ambient compensation sensor NTC on the VWM board in the detector has failed short circuit The resistance across the temperature compensation sensor NTC on the VWM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable Causes e Defective VWM board Suggested Actions V Exchange the VWM board 1200 Series Variable Wavelength Detector Service M anual 91 7 92 Error Information Fan Failed The cooling fan in the detector has failed The hall sensor on the fan shaft is used by the VWM board to monitor the fan speed If the fan speed falls below two revolutions second for more than five seconds the error message is generated Probable Causes e Fan cable disconnected e Defective fan Defective VWM board Suggested Actions V Ensure the fan is connected correctly V Exchange fan V Exchange the VWM board 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Open Cover The top foam has been removed The sensor on the VWM board detects when the top foam is in place If the foam is removed during operation the lamp and grating drive power is switched off and the error message is generated Probable Causes e Top foam removed Suggested Actions V Replace the
141. per kit includes 8 for optical unit plus 4 for fan G1314 65021 Figure 61 Optical Unit and Fan Assembly 242 1200 Series Variable Wavelength Detector Service M anual Parts for Repairs 14 Optical Unit Assembly Table 29 Optical Unit Item Description Part Number Optical Unit exchange part 61314 69060 2 Deuterium Lamp 61314 60100 3 Lens Assembly G1314 65008 for details of Lens Assembly see page 245 4 Filter Assembly Top G1314 65010 for details of Filter Assembly see page 245 5 Slit Kit includes Test Slit and Standard Slit 79853 68746 6 Grating Assembly includes Test Slit and Seal for Optical Cover G1314 65007 for details of Grating Assembly see page 246 7 M1 Mirror Assembly same for M1 and M 2 includes G1314 65012 8 M2 Test Slit and Seal for Optical Cover 9 ADC Board Assembly Sample G1314 67503 10 Photodiode Assembly Sample G1314 65014 11 Beam Splitter Assembly includes Test Slit and Seal for Optical G1314 65013 Cover 12 Photodiode Assembly Reference G1314 65015 13 ADC Board Assembly Reference G1314 67504 1200 Series Variable Wavelength Detector Service M anual 243 14 Parts for Repairs Figure 62 244
142. place the sample ADC board unsolder it from the 6 Replace the sample ADC board and solder it to the diode diode Left pin is GND Fix the ADC board ma 7 To replace the reference diode unscrew the ADC board 8 Replace the diode and solder it Upper diode pin is GND and unsolder the wire at the diode and unscrew diode 206 1200 Series Variable Wavelength Detector Service M anual Repair 12 9To replace the reference ADC board unsolder it from the diode 10 Replace the reference ADC board and solder it to the diode Upper pin is GND 12 Replace the optical unit see Installing the Optical Unit on page 223 13 Re align the reference slit see Optimizing the Reference Readings on page 198 1200 Series Variable Wavelength Detector Service M anual 207 12 Repair Replacing the Grating M otor or Position Sensor When required The grating motor or the position sensor might be replaced when the motor does not turn or the grating position is not sensed correctly Tools required Screwdriver POZI 1 PT3 Wrench 1 4 1 5 inch Hexagonal wrench 1 5 mm a pointed pair of pliers Parts required Grating M otor G1314 65009 includes test slit and seals for holes in cover Gratin
143. play a screen where you can enter the product number and or serial number 4 Make your changes using the information from the product label of your detector To configure the detector as G1314C VWD SL requires the correct main board version 1200 Series Variable Wavelength Detector Service M anual Repair 12 If you enter the wrong type your module might not be accessible anymore with the Agilent ChemStation and the Instant Pilot unsupported module In such a case follow the Recover Instructions on page 171 Press OK to highlight the complete command Press Done to transfer the information into the main board s memory Press Cancel quit the process Turn the detector OFF then ON again The Maintenance screen should display the correct serial number for this module If an Agilent ChemStation is also connected restart the Agilent ChemStation now as well Recover Instructions Turn off the detector Change the 8 bit Configuration Switch to Resident see Stay Resident Settings on page 299 3 Turn the detector on Re do steps 2 to 5 of Using the Instant Pilot G2408A on page 170 and correct the type information Enter the product number without R 5 Turn the detector off 6 Change the 8 bit Configuration Switch back to default settings see Setting the 8 bit Configuration Switch on page 295 Turn the detector ON again The Maintenance screen should display the correct type for this module
144. pler RS 232C The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module next to the GPIB connector The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Table 48 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI 1200 Series Variable Wavelength Detector Service M anual 293 16 Hardware Information Instrument PC RX 2 2 RX TX 3 x 3 TX DTR 4 4 DTR eD 5 5 GND DSR 6 6 DSR RTS 7 T 7 RTS CTS 8 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 78 RS 232 Cable 294 1200 Series Variable Wavelength Detector Service M anual Hardware Information 16 Setting the 8 bit Configuration Switch The 8 bit configuration switch is located next to the GPIB connector Switch settings provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures Factory setting is shown for the variable wavelength detector Figure 79 8 bit Configuration Switch Table 49 8 bit Configuration Switch Mode Select 1 2 3 4 5 6 7 8 GPIB 0 0 GPIB address RS 232C 0 1 Baud rate Data Parity bits Reserved 1 0 Reserved T
145. pply to normal operation if the cause of the overload condition has been removed An over temperature sensor in the main power supply is used to turn OFF output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument OFF wait until it is approximately at ambient temperature and turn the instrument on again The following table gives the specifications of the main power supply Table 57 Main Power Supply Specifications Maximum power 130W Continuous output Line input 100 240 volts AC Wide ranging 10 line frequency of 50 60 Hz Output 1 24V 4 5 A maximum Total power consumption of 24 V and 36 V must not exceed 107 W Output 2 36V 2 5 A maximum Output 3 5V 3A Output 4 15V 03A Output 5 15V 03A 1200 Series Variable Wavelength Detector Service M anual 301 16 Hardware Information 302 1200 Series Variable Wavelength Detector Service M anual This chapter provides addition information on safety legal and web Agilent 1200 Series Variable Wavelength Detector Service M anual A Appendix General Safety Information 304 Lithium Batteries Information 307 Radio Interference 308 SoundEmission 309 UV Radiation 310 Solvent Information 311 Declaration of Conformity for HOX2 Filter 313 Agilent Technologies on Internet 314 aa Agilent Technologies 303 A Appendix
146. procedure below 13 Check the firmware revision of the module If the firmware revision is older than the current firmware revision of the module update the firmware using the standard firmware update procedure see Replacing the Detector s Firmware on page 151 14 The detector requires a wavelength recalibration to write down the calibration parameters into the board s memory see Wavelength Verification Calibration on page 105 168 1200 Series Variable Wavelength Detector Service M anual Repair 12 Changing the Type and Serial Number When required If detector main board has been replaced Tools required User interface Parts required none Preparations Turn the detector on e Start the user interface When the main board has to be replaced the new board does not have a serial number For some modules e g pumps or auto samplers the type has to be changed multiple usage boards Use the information from the serial number plate of your module The changes become active after the reboot of the module Using the Agilent ChemStation Module serial numbers are entered by typing specific commands on the command line at the bottom of the main user interface screen 1 To enter a module serial number type the following command into the command line print sendmodule lvwd ser YYYYYYYYYY Where YYYYYYYYYY is the 10 character serial number of the module in question The first two characters are letter
147. r Standard Flow Cell 10 mm 14 ul 40 bar G1314 60086 1 Cell screw kit quantity 2 G1314 65062 2 Conical spring kit quantity 10 79853 29100 3 Ring 1 PEEK kit quantity 2 G1314 65065 4 Gasket 1 small hole KAPTON quantity 10 61314 65063 5 Window quartz kit quantity 2 79853 68742 6 Gasket 2 large hole KAPTON quantity 10 G1314 65064 7 Ring 2 PEEK kit quantity 2 G1314 65066 1 Cell Screw 2 Conical Springs 3 Ring 1 PEEK 4 Gasket 1 small hole 5 Window Quartz 6 Gasket 2 large hole 7 Ring 2 PEEK 7 2 eS 1 Figure 54 Standard Flow Cell 1200 Series Variable Wavelength Detector Service M anual 231 13 Parts and Materials for Maintenance Micro Flow Cell Table 21 Micro Flow Cell Assembly Item Description Part Number Micro flow cell 5mm 1 ul 40 bar 61314 60081 Capillary column detector SST 400 mm lg 0 12 i d 5021 1823 1 Cell screw 79853 27200 Cell kit micro comprises G1314 65052 two windows two gaskets 1 and two gaskets 2 2 Conical spring kit quantity 10 79853 29100 3 Ring SST kit quantity 2 79853 22500 4 Window quartz kit quantity 2 79853 68742 5 Gasket 1 PTFE quantity 10 79853 68743 6 Gasket 2 PTFE quantity 10 G1314 65053 1 Cell Screw 2 Conical Springs 3 Ring SST 4 Gasket 1 5 Window Quartz 6 Gasket 2 Figure55 Micro Flow Cell 232 1200 Series Variable Wavelength Detector Service M anual Parts and Materials for Maintenance 13 Semi
148. r Service M anual Repair 12 3 Unscrew the filter motor assembly and remove it 2 Locate the filter motor 5 To replace the sensor unscrew it and replace it by the new one 4 Unscrew the filter top part and place it safe fe position sensor s7 1200 Series Variable Wavelength Detector Service M anual 213 12 Repair 6 To replace the filter motor assembly unscrew the sensor for reuse on the new filter motor assembly G position C a N sensor T 7 Replace the filter top part and place it safe OT filter top TA WO 3 Ti W os 9 Route the cables through the openings and replace the cover 214 1200 Series Variable Wavelength Detector Service M anual Repair 12 10 Replace the cover I wi W y Continue as described in Exchanging the Filter Assembly on page 182 1200 Series Variable Wavelength Detector Service M anual 215 12 Repair Exchanging the Leak Sensor When required If defective Tools required Screwdriver POZI 1 PT3 Parts required Leak sensor assembly 5061 3356 Turn OFF the lamp Switch OFF the detector and disconnect the cables and capillaries Remove the detector from the stack and place it on the working bench PWN Pe Remove the front cov
149. r is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF WARNING To disconnect the detector from line unplug the power cord The power supply still uses some power even if the power switch at the front panel is turned OFF The detector was shipped with default configuration settings To change these settings Setting the 8 bit Configuration Switch on page 295 1200 Series Variable Wavelength Detector Service Manual 39 3 Flow Connections to the Detector Installing the Detector Preparations Parts required Detector is installed in the LC system Other modules Parts from accessory kit see Accessory Kit Contents on page 33 Two wrenches 1 4 5 16 inch for capillary connections When working with solvents please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when using toxic or hazardous solvents The flow cell is shipped with a filling of isopropanol also recommended when the instrument and or flow cell is shipped to another location This is to avoid breakage due to subambient conditions 40 1Press the release buttons and remove the front cover to have access to the flow cell area 2 Remove the me
150. racket and fix the cuvette 5 Install the cuvette holder in the instrument Next steps Perform your verification 1200 Series Variable Wavelength Detector Service M anual 147 11 Maintenance Correcting Leaks When required If a leakage has occurred in the flow cell area or at the capillary connections Tools required Tissue Two 1 4 inch wrenches for capillary connections Parts required None 1 Remove the front cover 2 Use tissue to dry the leak sensor area 3 Observe the capillary connections and the flow cell area for leaks and correct if required 4 Replace the front cover Waste outlet Leak sensor assembly Figure 42 Drying the Leak Sensor 148 1200 Series Variable Wavelength Detector Service M anual Maintenance 11 Replacing Leak Handling System Parts When required If the parts are corroded or broken Tools required None Parts required Leak funnel 5061 3356 Leak funnel holder 5041 8389 Leak tubing 120 mm 0890 1711 Remove the front cover to have access to the leak handling system Pull the leak funnel out of the leak funnel holder Pull the leak funnel with the tubing out of its location Replace the leak funnel and or the tubing Insert the leak funnel with the tubing in its position Insert the leak funnel into the leak funnel holder y Hm UU FP WwW N Replace the front cover Leak funnel holder p Leak funnel HE zg JS Ou E Leak tubing
151. ration or Control M odule G1323B B 04 02 and above optional for basic operation see note below System should be correctly set up for LAN communication with the Agilent ChemStation Zorbax Eclipse XDB C18 4 6 x 150 mm 5 um Part No 993967 902 or Part No 5063 6600 Part No 01080 68704 0 15 wt dimethylphthalate 0 15 wt diethylphthalate 0 01 wt biphenyl 0 03 wt o terphenyl in methanol The G1314C VWD SL can be operated with a G1323B Control M odule just in standard mode as G1314B no higher data rate selection is available 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 Conditions A single injection of the isocratic test standard is made under the conditions given in Table 8 Table8 Conditions Flow 1 5 ml minute Stoptime 8 minutes Solvent 100 30 water 70 Acetonitrile Temperature Ambient Wavelength sample 254 nm Injection Volume lul 1200 Series Variable Wavelength Detector Service M anual 47 4 48 Using the Detector Typical Chromatogram A typical chromatogram for this analysis is shown in Figure 9 The exact profile of the chromatogram will depend on the chromatographic conditions Variations in solvent quality column packing standard concentration and column temperature will all have a potential effect on peak retention and response DADI A Sigmi 4 ReeetN0 100 9901 8 94003 D mau 4 Figure9 Typical Chromatogram wit
152. required peakwidth depends on the peaks in the chromatogram see page 65 time table for programmable actions during the run Zero Offset Limits 1 to 99 in steps of 1 Attenuation Limits 0 98 to 4000 mAU at discrete values for either 100 mV or 1 V full scale additional signals can be stored with the normal signal for diagnostics autobalance to zero absorbance on the analog output plus offset at begin and or end of run see Special Setpoints on page 64 Figure 15 Detector Settings default 54 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 11 Pump the water acetonitrile 30 70 mobile phase through the column for 10 minutes for equilibration 12 Click the button and select Change to open the Signal Plot information Select the Pump Pressure and the VWD A Signal 254 as signals Change the Y range for the VWD to 1 mAU and the offset to 20 and the pressure offset to 50 The X axis range should be 15 minutes Press OK to exit this screen Sl nnn Sc ao racs G Fl omg Ma ATA O vailable Signals Selected Signals A A A A Edit Signal Plot OEFAULT O Quaternary Pump Pressure Flow uaternary Pump 4 uaternary Pump B uaternary Pump oC waternary Pump D olumn Thermostat Temperature l olumn Thermostat Temperature ri WD B Signal w o Reference at 254 VWD A Absorbance at 254 nm Add gt E data
153. rformance The detector has a variety of parameters that can be used to optimize performance The information below will guide you on how to get the best detector performance Follow these rules as a start for new applications It gives a rule of thumb for optimizing the detector parameters Match the Flow Cell to the Column Figure 26 recommends the flow cell that matches the column used If more than one selection is appropriate use the larger flow cell to get the best detection limit Use the smaller flow cell for best peak resolution Column length Typical peak Recommended flow cell width lt 5cm 0 025 min Semi micro 10cm 0 05 min flow cell 20cm 0 1 min plandary flow cell pzmm joome C a Typical flow rate 0 05 0 2 ml min 0 2 0 4ml min 0 4 0 8 ml min 1 2 ml min Internal column diameter Figure 26 Choosing a Flow Cell 70 1200 Series Variable Wavelength Detector Service M anual How to optimize the detector 5 Flow Cell Path Length Lambert Beer s law shows a linear relationship between the flow cell path length and absorbance I Absorbance logT log C d where T is the transmission defined as the quotient of the intensity of the transmitted light divided by the intensity of the incident light l is the extinction coefficient which is a characteristic of a given substance under a precisely defined set of conditions of wavelength solvent temperature
154. ries Variable Wavelength Detector Service M anual 19 1 Introduction to the Variable Wavelength Detector Security lever Slot for interface _ board 7 CAN GPIB serial number DE manufactured in Germany 6 2006 Configuration switch 01 week of last major change 00130 number of unit product number 613148 VWDSL 613148 VWD SL serial number Serial No Serial No wA Ani i CE ums safety standards He Agilent Technologies Pil X y LASI70 002751 ICES NMB ON oo configuration switch we 1f 2fafe s s e settings referto Setting esma e 1 tesdrate 9 pany the 8 bit Configuration Switch on page 295 tee 120 220 200 v VOltage range mva sa Dower consumption frequency Agilent Vechnotogers 76337 Waldbronn Made in Germany Figure4 Rear View of Detector Electrical Connections and Label NOTE The G1314C VWD SL has no GPIB connector 20 1200 Series Variable Wavelength Detector Service M anual Introduction to the Variable Wavelength Detector 1 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers foam plastic spacers in which the mechanic
155. rmware Update Tool The installation of older firmware might be necessary e to keep all systems on the same validated revision or e if third part control software requires a special version To upgrade downgrade the detector s firmware the following steps have to be performed 1 Download the module s firmware the LAN RS 232 FW Update Tool Version 2 00 or above and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 Load the firmware into the detector as described in the documentation The G1314C VWD SL requires firmware revision A 06 02 or above main and resident 1200 Series Variable Wavelength Detector Service M anual 151 11 Maintenance Tests amp Calibrations The following tests are required after maintenance of lamps and flow cells e Intensity Test on page 153 e Wavelength Verification Calibration on page 155 e Holmium Oxide Test on page 156 152 1200 Series Variable Wavelength Detector Service M anual Intensity Test Maintenance 11 The intensity test measures the intensity of the deuterium lamp over the full VWD wavelength range 190 600 nm The test can be used to determine the performance of the lamp and to check for dirty or contaminated flow cell windows When the test is started the gain is set to zero To eliminate effects due to absorbing solvents the test should be done with water in the flow cell The shape of the intensity spectru
156. s which should be capitalized The reply line will respond with RA 0000 SER followed by the module serial number you just entered To change the type of the module use the following command 1200 Series Variable Wavelength Detector Service M anual 169 12 Repair print sendmodule lvwd TYPE XXXXX Where XXXXX is the 5 character product number of the module e g G1314B To configure the detector as G1314C VW D SL requires the correct main board version WARNING If you enter the wrong type your module will not be accessible anymore In sucha case see Using the Instant Pilot G2408A on page 170 or Using the Control M odule G1323B on page 171 for recovering 170 2 Turn OFF the detector then ON again Then restart the Agilent ChemStation If the serial number you have just entered is different than the original module serial number you will be given the opportunity to edit the configure 1200 access screen during the restart of the Agilent ChemStation 3 After restart the serial number type you have just entered can be seen under the Instrument menu of the main user interface screen Using the Instant Pilot G2408A 1 Connect the Instant Pilot to the detector Turn ON the detector 2 On the Instant Pilot s Welcome screen press More then select Maintenance Using the up down arrows select the detector where you have to change the product number or serial number 3 Press PN SN This will dis
157. should be performed with a clean and bubble free flow cell and with a new deuterium lamp or one in good condition In case of the following problem symptoms call your local Agilent Technologies service representative for help e low intensity readings after replacement of lamp and or cleaning of the flow cell and or source lens assembly e calibration failures zero order holmium oxide lines or 656 nm emission lines e excessive noise and or drift e grating drive failures e filter drive failures and e no intensity readings on one or both the reference and or sample side 1200 Series Variable Wavelength Detector Service M anual 175 12 Repair Special Notes The following procedures describe the replacements of parts separately During these procedures the instrument is turned on Do not touch the processor board nor the lamp cables It is important that only one assembly mirror grating beam splitter is changed and calibrated at a time Otherwise you will lose correct optical assembly alignment during the calibration process Do not remove the Entrance Slit Holder nor loosen it Otherwise the optical unit has to be exchanged completely 176 The photo current readings with test slit installed are much lower than with the standard slit If mirrors or grating show signs of contaminations read Contaminated M irrors and Grating on page 177 first 1200 Series Variable Wavelength Detector Service M
158. sponse time 2 seconds 1200 Series Variable W avelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual Pa 6 ee 9 e Diagnosis Signals Diagnostic Signal Output 122 Service Dialog 124 This chapter describes the detector s built in diagnostics ott Agilent Technologies 121 9 Diagnosis Signals Diagnostic Signal Output 122 The detector provides an analog signal output for various detector signals for diagnostic purposes Selecting and Plotting Signals Some of the signals are available on the analog output only when running specific tests see following sections Only one signal can be plotted at any one time All values in Table 17 on page 125 refer to the following settings on the user interface attenuation 1000 mAU zero offset 5 analog voltage range 1 V full scale The following signals are available on the analog signal output and the control display select Analog Out VWD as source signal in the Plot Setup For signal descriptions see Signal Descriptions on page 126 Table 16 Diagnostic Signals on Analog Output Signal Analog Out Display 1V is equal to Constant zero offset 0 mV Absorbance 1AU AU Sample signal without reference 1AU AU Reference signal without sample 1AU AU Sample raw data 27 counts Counts unfiltered and not logarithmic Reference raw data 221 counts Counts unfiltered and not logarithmic 1200 Series
159. ssembly 1200 Series Variable Wavelength Detector Service M anual 247 14 Parts for Repairs Filter Assembly Do not disassemble the Filter Assembly Its height is factory aligned Table 32 Filter Assembly Item Description Part Number 1 Filter Assembly Top G1314 65010 2 Washer see 3 Screw M3 12 mm lg see 4 Filter M otor Assembly includes items 4 5 and 6 G1314 65011 5 Filter Bottom 6 Plate Miscellaneous Kit includes screws washers and spacers G1314 65022 used for Grating and Filter Assembly 248 1200 Series Variable Wavelength Detector Service M anual Parts for Repairs 14 3 er rr U 2 Figure 65 Filter Assembly 1200 Series Variable Wavelength Detector Service M anual 249 14 Parts for Repairs Lens Assembly Table 33 Lens Assembly Item Description Part Number Lens Assembly complete G1314 65008 1 Lens G1314 65020 2 Lens Screw 3 Lens Holder 3 Figure 66 Lens Assembly 250 1200 Series Variable Wavelength Detector Service M anual Parts for Repairs 14 Sheet M etal Kit Table 34 Sheet Metal Kit Parts Item Description Part Number Sheet metal kit G1314 65017 includes items 1 2 and 3 1 Top cover Case Plate AJW N Cover for lamp G1314 65018 Figure 67 Sheet Metal Kit Parts 1200 Series Variable Wavelength Detector Service M anual 251 14 Parts for Repairs Plastic Parts 252 Table 35 Plastics Parts
160. ssion at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1200 Series Variable Wavelength Detector Service M anual 309 A Appendix UV Radiation 310 Emissions of ultraviolet radiation 200 315 nm from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs Threshold Limit Values according to the American Conference of Governmental Industrial Hygienists Table 59 UV Radiation Limits Exposure day Effective Irradiance 8 hours 0 1 uW cm 10 minutes 5 0 uW cm Typically the radiation values are much smaller than these limits Table 60 UV Radiation Typical Values Position Effective Irradiance Lamp installed 50 cm distance average 0 016 uW cm Lamp installed 50 cm distance maximum 0 14 pW cm 1200 Series Variable Wavelength Detector Service M anual Appendix A Solvent Information Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Prevent any crystallization of buffer solutions This will lead into a blockage damage of the flow cell If the flow cell is transported while temperatures are below 5 degree C it must be assured th
161. stable is vertically and horizontally Both mirrors are identical Grating Assembly The grating separates the light beam into all its component wavelengths and reflects the light onto mirror 2 Beam Splitter Assembly The beam splitter splits the light beam One part goes directly to the sample diode The other part of the light beam goes to the reference diode Photo Diodes Assemblies Two photo diode assemblies are installed in the optical unit The sample diode assembly is located on the left side of the optical unit The reference diode assembly is located in the front of the optical unit Photo Diode ADC analog to digital converter The photo diode current is directly converted to 20 bit digital data direct photo current digitalization The data is transferred to the detector main board VWM The photo diode ADC boards are located close to the photo diodes 1200 Series Variable Wavelength Detector Service M anual Introduction to the Variable Wavelength Detector 1 Electrical Connections The GPIB connector G1314B only is used to connect the detector with a computer The address and control switch module next to the GPIB connector determines the GPIB address of your detector The switches are preset to a default address Table 46 on page 291 or Table 50 on page 296 and is recognized once after power is switched ON e The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are us
162. stallation Ethanol or M ethanol Alternative to Isopropanol second choice if no Isopropanol is available To clean the system when using buffers Bidistilled water Best solvent to re dissolve buffer crystals After a solvent change Bidistilled water Best solvent to re dissolve buffer crystals After the installation of normal phase Hexane 5 Isopropanol Good wetting properties seals P N 0905 1420 The pump should never be used for priming empty tubings never let the pump run dry Use a syringe to draw enough solvent for completely filling the tubings to the pump inlet before continuing to prime with the pump 1 Open the purge valve of your pump by turning it counterclockwise and set flow rate to 3 5 ml min 2 Flush all tubes with at least 30 ml of solvent 3 Set flow to required value of your application and close the purge valve Pump for approximately 10 minutes before starting your application 1200 Series Variable Wavelength Detector Service M anual 45 4 46 Using the Detector Requirements and Conditions What You Will Need Table 7 lists the items you need to have for the set up of the analysis Some of these are optional not required for the basic system Table 7 1200 system Column Standard What you will need Pump plus degassing Autosampler Detector standard flow cell installed Agilent ChemStation B 02 01 and above or Instant Pilot G4208 A 01 01 and above optional for basic ope
163. t Preparations for this procedure l Carefully fit the top foams into the detector All previous repairs have been completed 1200 Series Variable Wavelength Detector Service M anual 225 12 Repair 2 Slide the top plate towards the rear and fix the top plate 3 Replace the cover screws 5 Replace the waste funnel and the front panel Next steps Replace the detector into the stack Reconnect the cables and capillaries and turn ON the detector 226 1200 Series Variable W avelength Detector Service M anual Repair 12 Assembling the M ain Cover When required If cover is broken Tools required None Parts required Plastics kit 5065 9982 includes base top left and right sides The plastics kit contains all parts but it is not assembled WARNING In case you insert the left or right side in the opposite position you may not be able to remove the side from the top part 1 Place the top part on the bench and insert the left and right 2 Replace the cover side into the top part Next steps Replace the detector into the stack and reconnect the cables and capillaries Turn ON the detector 1200 Series Variable Wavelength Detector Service M anual 227 12 Repair 228 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 13 Parts and Materials for Ma
164. t 85 VDC for stable operating conditions and light emission of the deuterium lamp Igniting the Deuterium Lamp The deuterium lamp filament is heated for several seconds prior to ignition The deuterium lamp current control circuit gives an ignition pulse to the lamp resulting in lamp ignition The filament control circuit disables or lowers the filament voltage depending on the lamp type if the lamp was ignited successfully 1200 Series Variable Wavelength Detector Service M anual Hardware Information 16 If the deuterium lamp failed to ignite the whole sequence is repeated after a wait sequence for cooling down If the deuterium lamp still does not ignite an error message occurs Diagnostic A D Converter The diagnostic A D converter senses currents and voltages of the deuterium and tungsten lamps and converts the analog signals into digital values The digital values are transferred via the control bus on the VWM board When values are outside of the normal range an appropriate error message is generated Safety Switch If the rear EPP foam is removed while the instrument is still on the safety light switches are activated and the deuterium lamp the grating drive and the fan are switched OFF 1200 Series Variable Wavelength Detector Service M anual 283 16 Hardware Information Board Layout and Connectors J 1 2 CAN J 3 Interface board J 5 Test connector OTT
165. t on the working bench Remove the front cover top cover and top foam section see Removing the Top Cover and Top Foams on page 163 ww 1200 Series Variable Wavelength Detector Service M anual 17 12 Repair 2 Remove the bumper from the fan 7 Fit the fan assembly into its location Replace and 3 Remove the fan protection grille reconnect the fan assembly to the main board 4 Exchange the fan 5 Refit the fan protection grille newer grilles are just clipped 6 Replace the bumpers Note Next steps The fan must be installed in the correct orientation to Reinstall the top foam section top cover and front ensure optimum cooling and operation of the cover see Installing the Foam and the Top Cover on detector The direction of air flow is from the front page 225 towards the rear Replace detector into the stack Reconnect the cables and turn ON the detector 174 1200 Series Variable W avelength Detector Service M anual Repair 12 Repairs in the Optical Unit Inside the optical unit the following assemblies may be replaced by detector users e the source lens assembly described on page 179 and e the filter assembly described on page 182 All other repairs within the optical unit require special knowledge and training Therefore these repairs and calibrations must be performed by Agilent Technologies trained service engineers Troubleshooting
166. t pipe 5041 8382 2 Status light pipe 5041 8384 3 Power switch button 5041 8381 4 Coupler for power supply actuator 5041 8383 4 Figure 71 Power and Status Light Pipes 1200 Series Variable Wavelength Detector Service M anual 255 14 Parts for Repairs 256 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 15 Identifying Cables Cable Overview 258 Analog Cables 260 Remote Cables 263 BCD Cables 268 Auxiliary Cable 270 CAN Cable 271 External Contact Cable 272 RS 232 Cable Kit 273 LAN Cables 274 This chapter provides information on cables used with the 1200 series of HPLC modules ott Agilent Technologies 257 15 Identifying Cables Cable Overview WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Table 39 Cables Overview Type Description Part Number Analog 3390 2 3 integrators 01040 60101 cables 3394 6 integrators 35900 60750 35900A A D converter 35900 60750 General purpose spade lugs 01046 60105 Remote 3390 integrator 01046 60203 cables 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series integrator 03394 60600 3396 Series II 3395A integrator see page 265 3396 Series III 3395B integrator 03396 61010 Agilent 1200 1050 modules 1046A FLD 5061 3378 1046A FLD 5061 3378 35900A A D
167. tal cover and install the flow cell Tighten the cell screws 1200 Series Variable Wavelength Detector Service M anual Installing the Detector 3 3 Assemble the column detector capillary Depending on the flow cell type it may be a PEEK or SST capillary 4 Connect the newly assembled fitting of the capillary to the inlet connector 5 Connect the other end of the capillary to the column 6 Connect the PEEK waste capillary to the outlet connector 1200 Series Variable Wavelength Detector Service M anual 41 3 Installing the Detector 7 Establish a flow and observe for leakage 8 Replace the front cover 42 The installation of the detector is now complete The detector should be operated with the front cover in place to protect the flow cell area against strong drafts from the outside 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual Pa 6 ee 4 Using the Detector Setting up an Analysis 44 Before Using the System 44 Requirements and Conditions 46 Optimization of the System 48 Preparing the HPLC System 49 Running the Sample and Verifying the Results 58 Special Settings of the Detector 59 Control Settings 59 Online Spectra 60 Scanning with the VWD 61 Analog Output Settings 63 Peakwidth Settings 65 Optimizing the Detector 67 This chapter provides in
168. tector Service M anual 63 4 Using the Detector Special Setpoints set up wWD Signal 5 D of Control Eu Online Spectra 1 To change the offset and the attenuation select VWD Signal More Special Setpoints WD Special Setpoints System 2 x 8 Not Ready Information Help 64 Margin for negative Absorbance Signal Polarity Positive Negative Tl Enable analysis when lamp is off Restore Defaults m U Scan Range fig0 to 400 nm Step j2 nm Cancel Figure 24 Spectra Window Help Margin for negative Absorbance Use this field to modify the detector s signal handling to increase the margin for negative absorbance Use this option if for example your solvent gradient produces a decreasing baseline absorbance and for GPC analyses Limits 100 to 4000 mAU Signal Polarity can be switched to negative if required Enable analysis when lamp is off if the VWD is not used in a dual detector setup lamp off the not ready condition is not stopping the analysis Scan Range Step Used for stop flow scanning see Scanning with the VW D on page 61 Margin for negative Absorbance The higher the value the greater the baseline noise Set this value only if you expect negative absorbance greater than 100 mAU 1200 Series Variable Wavelength Detector Service M anual Using the Detector 4 Peakwidth Settings Do not use peak width shorter than
169. tenuation 54 autobalance 54 automatic turn on 59 data rate 66 detector parameters 53 drift 51 EMF 22 enable analysis when lamp is off 64 error method 59 isocratic test sample 58 lamp type 59 load method 52 negative absorbance margins 64 online plot 56 online spectra 60 peakwidth setting 54 peakwidth settings 65 preparing the HPLC system 49 priming and purging the system 44 requirements and conditions 46 1200 Series Variable Wavelength Detector Service M anual response time 66 running the sample 58 sample info 57 scan range step 64 scanning 61 setting up an analysis 44 setup of detector 53 signal plot 55 signal polarity 64 special setpoints 54 64 special settings 59 stable baseline 51 stop and post time 54 stop flow condition 60 the cuvette holder 145 the detector 43 time table 54 turn on 50 typical chromatogram 48 warmup 51 zero offset 54 UV radiation 310 WwW warm up 51 warnings and cautions 131 161 wavelength accuracy 29 calibration 105 155 range 190 600 nm 29 recalibration 76 weight 28 Z zero offset 54 1200 Series Variable Wavelength Detector Service M anual Index Index 6 1200 Series Variable Wavelength Detector Service M anual www agilent com In This Book This manual contains the technical reference information about the Agilent 1200 Series variable wavelength detector The manual describes the following
170. the operational state prerun run and error states of the detector The status indicators provide a quick visual check of the operation of the detector see page 51 Error Messages In the event of an electronic mechanical or hydraulic failure the detector generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see Error Information on page 81 Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Test Functions on page 103 Wavelength Verification Recalibration Wavelength recalibration is recommended after repair of internal components and on a regular basis to ensure correct operation of the detector The detector uses the deuterium alpha and beta emission lines for wavelength calibration see Wavelength Verification Calibration on page 105 Diagnostic Signals The detector has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing baseline problems see Diagnosis Signals on page 121 1200 Series Variable Wavelength Detector Service M anual Troubleshooting and Diagnostics 6 Status Indicators Two status indicators are located on the front of the detector The lower left indicates the power supply
171. the CAN 38 connecting the ChemStation 36 connecting the LAN 38 connecting the power 36 38 overview and identification 258 calibration 656 nm 106 zero order 106 N cables parts 271 connecting interface 38 interface 291 cautions and warnings 131 161 chromatogram 48 configuration stack 34 configuration switch default settings 295 description and factory setting 295 connector locations at rear 38 contaminated mirrors and grating 177 control module G1323B 14 79 correction factors for flow cells 72 cutoff filter 17 277 cuvette holder 145 D DAC test 116 dark current test 114 data rate 66 73 declaration of conformity 313 default address GPIB 291 296 delivery checklist 32 detection type 29 1200 Series Variable Wavelength Detector Service M anual Index detector error messages 94 detector main board VWM 280 diagnostic A D converter 283 board temperature 127 signal descriptions 126 signals 76 signals overview 122 125 test functions 103 dimensions 28 drift 29 51 initial 56 electrical connections descriptions of 19 EMF early maintenance feedback 22 enable analysis when lamp is off 64 entrance slit assembly 17 277 environment 27 error messages 76 82 calibration failed 98 compensation sensor open 90 compensation sensor short 91 detector 94 fan failed 92 general 83 grating filter motor defective 100 heater current missing 97 holmi
172. the cell in which UV absorption takes place and the intensity is converted to an electrical signal by means of the sample photodiode Part of the light is directed to the reference photodiode by the beam splitter to obtain a reference signal for compensation of intensity fluctuation of the light source A slit in front of the reference photodiode cuts out light of the sample bandwidth Wavelength selection is made by rotating the grating which is driven directly by a stepper motor This configuration allows fast change of the wavelength The cutoff filter is moved into the lightpath above 370 nm to reduce higher order light Deuterium lamp a Filter assembly Lens if Mirror M1 Sample diode l Grating Flow cell Mirror M2 Beam splitter Reference diode Figure1 Optical Path of the Variable Wavelength Detector 1200 Series Variable Wavelength Detector Service M anual 15 1 Introduction to the Variable Wavelength Detector Flow Cell A variety of flow cell cartridges can be inserted using the same quick and simple mounting system Figure 2 Cartridge Type Flow Cell Table1 Flow Cell Data STD Semi micro High Pressure Micro Maximum pressure 40 4 40 4 400 40 40 4 bar M Pa Path length 10 conical 6 conical 10 conical 5 mm Volume 14 5 14 1 ul Inlet i d 0 17 0 17 0 17 0 1 mm Inlet length 750 750 750 555 mm Outlet i d 0 25 0 25 0 25 0 25 mm Outlet length 120 120 120 120 mm Materi
173. the ignition cycle If the lamp current does not rise above the lower limit within 2 5 seconds the error message is generated Probable Causes e Lamp not connected e Defective or non Agilent lamp e Defective VWM board e Defective power supply Suggested Actions V Ensure the lamp is connected V Exchange the lamp V Exchange the VWM board V Exchange the power supply 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Heater Current Missing The lamp heater current in the detector is missing During lamp ignition the processor monitors the heater current If the current does not rise above the lower limit within one second the error message is generated Probable Causes e Lamp not connected e Ignition started without the top foam in place e Defective or non Agilent lamp e Defective VWM board e Defective power supply Suggested Actions V Ensure the lamp is connected V Replace the top foam and turn on the lamp V Exchange the lamp V Exchange the VWM board V Exchange the power supply 1200 Series Variable Wavelength Detector Service M anual 97 7 98 Error Information Calibration Failed The intensity maximum was not found during wavelength calibration Calibration 0 Failed Zero order calibration failed Calibration 1 Failed 656 nm calibration failed During zero order and 656 nm calibration the detector searches for the intensity maximum If the ma
174. tly viewing the light produced by the deuterium lamp used in this product Always turn the deuterium lamp off before removing it 1200 Series Variable Wavelength Detector Service M anual 131 10 Maintenance and Repair Cleaning the Detector The detector case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth allowing liquid to drip into the detector WARNING Do not let liquid drip into the detector It could cause shock hazard and it could damage the detector 132 1200 Series Variable Wavelength Detector Service M anual Maintenance andRepair 10 Using the ESD Strap Electronic boards are sensitive to electrostatic discharge ESD In order to prevent damage always use an ESD strap when handling electronic boards and components 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 39 Using the ESD Strap 1200 Series Variable Wavelength Detector Service M anual 133 10 Maintenance and Repair 134 1200 Series Variable Wavelength Detector Service M anual Agilent 1200 Series Variable Wavelength Detector Service M anual 11 Maintenance Overview of Maintenance 136 Exchangi
175. to the main board QO PEE HHHH TAE a THH a eo 16 Reinstall the processor board see Exchanging the Main Board on page 166 17 Reinstall the Fan Assembly see Exchanging the Fan on page 173 18 Reinstall the optical unit see Replacing Status Light Pipe on page 222 220 1200 Series Variable Wavelength Detector Service M anual Repair 12 19 Reinstall the front cover top cover and top foam section see Installing the Foam and the Top Cover on page 225 20 Replace the detector into the stack 21 Reconnect the power cable and turn ON the detector 1200 Series Variable Wavelength Detector Service M anual 221 12 Repair Replacing Status Light Pipe When required When part is broken Tools required Screwdriver POZI 1 PT3 Parts required Status light pipe 5041 8384 Preparations for this procedure are 1 The status light pipe is clipped into the top cover Remove the front cover and top cover Removing the Top Cover and Top Foams on page 163 2 Replace the foam section the top cover and front cover see Installing the Foam and the Top Cover on page 225 3 Replace the detector i
176. tor at the front of the module is switched on and an entry is written into the instrument logbook 82 1200 Series Variable Wavelength Detector Service M anual Error Information 7 General Error Messages General error messages are generic to all Agilent 1200 Series HPLC modules Timeout The timeout threshold was exceeded Probable Causes e The analysis was completed successfully and the timeOut function switched off the pump as requested e A not ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Suggested Actions V Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required 1200 Series Variable Wavelength Detector Service M anual 83 7 Error Information Shutdown An external instrument has generated a shut down signal on the remote line The detector continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable Causes e Leak detected in an external instrument with a remote connection to the system e Shut down in an external instrument with a remote connection to the system The degasser failed to generate sufficient vacuum for solvent degassing Suggested Actions V Fix the leak in the external instrument before restarting the pump V Check external instruments for a shut down condition
177. ty data sheet by the solvent vendor especially when using toxic or hazardous solvents The electronics of the detector will not allow operation of the detector when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the fan immediately Voltages for the other electronic components will be turned off after 30 seconds The status lamp will light up red and an error will be logged into the logbook of the user interface Always operate the detector with the top covers in place Electronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use an ESD protection for example the ESD wrist strap from the accessory kit when handling electronic boards and components see Using the ESD Strap on page 133 1200 Series Variable Wavelength Detector Service M anual 161 12 Repair WARNING Eye damage may result from directly viewing the light produced by the Deuterium lamp used in this product Always turn off the deuterium lamp before removing it 162 1200 Series Variable Wavelength Detector Service M anual Repair 12 Removing the Top Cover and Top Foams When required For all repairs inside the detector Tools required Screwdriver POZI 1 PT3 Parts required Depends on the work inside and the following procedures Preparations for this procedure 1 Press the release buttons and remove the front cover Tum OFF t
178. ual 271 15 Identifying Cables External Contact Cable O o Ta o 0 o 6 cdo o o ol 950 o o Off O One end of this cable provides a 15 pin plug to be connected to Agilent 1200 Series module s interface board The other end is for general purpose Agilent 1200 Series Interface Board to general purposes Connector Color Pin Signal Name G1103 61611 Agilent 1200 White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 ee Blue 7 EXT 4 S Red 8 EXT4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 272 1200 Series Variable Wavelength Detector Service M anual Identifying Cables 15 RS 232 Cable Kit This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter Use the cable and adapter to connect Aligent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1200 module to PC RS 232 Cable Kit 34398As Instrument PC RX 2 2 RX TX 3 X 3 TX DTR 4 4 DTR GND 5 gt 5 enD DSR 6 6 DSR RTS 7 7 RTS CTS 8 m e 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male 1200 Series Variable Wavelength Detector Service M anual 273 15 Identifying Cables LAN Cables Recommended Cables Table 40 Description Part number Cross over network cable shielded 3
179. uired Screwdriver POZI 1 PT3 Hexagonal wrenches 5mm 7mm and 15 mm Parts required Detector main board VWM exchange assembly G1314 69531 G1314C Detector main board VWM exchange assembly G1314 69525 G1314B The G1314C VWD SL no longer has a GPIB connection Therefore ignore all GPIB information 1 Turn OFF the lamp 2 Switch OFF the detector and disconnect the cables 3 Remove detector from stack and place it on the working bench Analog connector Board recess Board recess Figure 47 Unscrew Connectors From Board 4 Remove the front cover top cover and top foam section see Removing the Top Cover and Top Foams on page 163 1200 Series Variable Wavelength Detector Service M anual Repair 12 5 Use a5 mm and 7 mm wrench to unscrew the REMOTE and the GPIB connector and a 15 mm wrench to unscrew the analog connector nut 6 Disconnect all connectors from the processor board When removing connectors counter hold with one hand on connector J 3 J1 2 CAN J 3 Interface board J 5 Test connector urar EE Ly i J 12 RS 232C L_ of Ho J 15 REMOTE 1 J J2 JI J2 E J 17 GPIB J 150 Power supply J 201 Grating motor i i 203 Fan assembly J 204 Leak sensor J 205 Grating position J 207 Filter motor J5 E J 208 Analog output J 209 Filter position
180. um oxide test failed 99 ignition without cover 93 lamp current missing 94 lamp ignition failed 96 lamp voltage missing 95 leak 87 leak sensor open 88 leak sensor short 89 remote timeout 85 shutdown 84 synchronization lost 86 timeout 83 wavelength check failed 101 ESD electrostatic discharge strap 133 exchange interface board 150 Index exchanging parts see repairs 159 external contact cable parts 272 F factory settings 295 fan drive 281 parts 242 features 14 GLP 30 instrument layout 21 safety and maintenace 30 filter assembly parts 248 filter motor driver 281 firmware description 285 main system 285 resident system 285 updates 151 286 flow cell 16 279 choosing 70 correction factors 72 cuvette holder parts 236 high pressure parts 235 micro parts 232 path length 72 semimicro parts 233 standard parts 231 types and data 16 29 279 foams parts 254 front of detector 37 fuses on BCD board 287 G general error messages 83 GPIB default addresses 296 interface 291 grating assembly 18 278 assembly parts 246 contaminated 177 motor driver 281 motor test 117 H holmium oxide declaration of conformity 313 filter 17 277 test 109 HP J etDirect card 289 information on cuvette holder 145 on lithium batteries 307 on radio interference 308 on solvents 311 on sound emission 309 on UV radiation 310 instal
181. xcessive drift or noise or no response has been identified Tools required Screwdriver POZI 1 PT3 Wrench 1 4 1 5inch Hexagonal wrench 1 5 mm A pointed pair of pliers Soldering iron Parts required Photodiode Assembly Sample G1314 65014 Reference G1314 65015 ADC Board Assembly VWA Sample G1314 67503 Reference G1314 67504 Preparation for this procedure are 1 Locate the sample or the reference diode and ADC boards Install the Test Slit see Installing the Test Slit on page 185 Remove the optical unit from the instrument see Removing the Optical Unit on page 202 The top cover of the optical unit is removed For replacement of ADC boards VWA remove the bottom cover of the optical unit 204 1200 Series Variable Wavelength Detector Service M anual Repair 12 2 Remove the cover of sample or the reference side Reference To replace the reference side parts continue with step 7 3 To replace the sample diode unscrew the ADC board and unsolder the wire at the diode Unscrew the diode 4 Replace the diode and solder it Left diode pin is GND 1200 Series Variable Wavelength Detector Service M anual 205 12 Repair 5 To re
182. ximum is not detected within the scan range the error message is generated Probable Causes e Lamp is OFF Incorrect flow cell installation Flow cell contamination or air bubbles e Intensity too low e Current step value too far from maximum e Misalignment of the grating assembly e Defective grating assembly e Defective VWM board Suggested Actions V Switch on the lamp V Ensure the flow cell are installed correctly V Clean replace flow cell windows or remove air bubbles V Replace lamp Y Enter a different calibration step value different scan range Repeat the calibration Y Realign the grating V Run the grating motor test to determine if the grating assembly is defective Exchange the grating assembly if required V Exchange the VWM board 1200 Series Variable Wavelength Detector Service M anual Error Information 7 Holmium Oxide Test Failed The holmium oxide test in the detector has failed During the holmium test the detector moves the holmium filter into the light path and compares the measured absorbance maxima of the holmium oxide filter with expected maxima If the measured maxima are outside the limits the error message is generated Probable Causes e Defective or dirty flow cell e Defective filter motor assembly e Defective grating motor assembly Suggested Actions V Ensure the flow cell is inserted correctly and is free from contamination cell windows buffers and so

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