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1. 4 End Figure 4 2 Power On Auto Hematology Analyzer Service Manual V1 1 Hydraulic System 4 4 2 Whole Blood Count Ree Blood Count v Read HGB blank 50ul syringe aspirate 13ul blood sample v Rinse and drain WBC bath Dispense diluent prefill the WBC bath 1 269 dilution in WBC bath v ske Rinse and drain RBC bath y 50ul syringe aspirate 15 6ul blood dilution Dispense diluent prefill the RBC bath Add 0 5ml lyse reagent to the WBC bath v v 1 308 dilution in WBC bath 1 44833 dilution in RBC bath WBC Count v MI RBC PLT Count Read HGB sample v Rinse WBC bath v Rinse RBC bath v Zap the apertures v C End 5 si Figure 4 3 Whole Blood Count Auto Hematology Analyzer Service Manual V1 1 4 5 Hydraulic System 4 4 3 Prediluted Count Well mixed 1 80 prediluted sample k RE EJ ars Prediluted Mode Count v v 10ml syringe aspirate 0 7ml 1 80 prediluted sample Read HGB blank v Rinse and drain WBC bath Dispense diluent prefill the WBC bath v Add 0 36ml lyse reagent to the WBC bath v 1 407 dilution in WBC bath WBC Count v Read HGB sample
2. o N x V S mo Figure 4 10 Inspire Sample and Diluent Prediluted Mode 4 12 Auto Hematology Analyzer Service Manual V1 1 Hydraulic System 4 5 3 WBC Injection amp Lyse Preparation 17 10 iy 18 TA S 16 15 14 13 11 Figure 4 11 WBC Injection amp Lyse Preparation Auto Hematology Analyzer Service Manual V1 1 4 13 Hydraulic System 4 5 4 RBC amp Lyse Injection 17 10 iy 18 Lf TA 4 K 16 15 14 13 12 Figure 4 12 RBC 8 Lyse Injection 4 14 Auto Hematology Analyzer Service Manual V1 1 Hydraulic System 4 5 5 Mixture ee O a 1 a a 17 18 TA LL 14 13 Figure 4 13 Mixture Auto Hematology Analyzer Service Manual V1 1 4 15 Hydraulic System 4 5 6 Count Cycle 4 16 10 D a N a Nad A XA Ni N ne N Z ni gt 4 v a V VP lt Figure 4 14 Count Cycle Auto Hematology Analyzer Service Manual V1 1 16 12 14 iy 13 Hydraulic System 4 5 7 Cleaning Figure 4 15 Cleaning Auto Hematology Analyzer Service Manual V1 1 4 17 Hydraulic System 4 5 8 Flush 4 18 17 lt 9 m X N N 5 gt CD ilo to V Y Figure 4 16 Flush Auto Hematology Analyzer Service Manual V1 1 15 16 12 AT 13 4 5 9 Empty Tube 18 Hydraulic System 10 iy Vi 16 13 Figure 4 17 Empty
3. ENTER to ext Figure 10 39 Auto Hematology Analyzer Service Manual V1 1 10 13 Password and Upgrade Software Chapter 11 Password and Upgrade software 11 1 Password HEMATOLOGY ANALYZER software system set more than one password to realize different functions Password Functions Modify the Count Time in Setup menu Modify the Auto Clean Time in Setup menu 3000 Modify the Gain in Setup menu FON gt Control the rotatory motor and fluctuating motor to replace probe wipe block Run and print the factory calibration a Calibrate in Service System Status Test the running status of the motor in Service System Test 1 The functions of the expanded keys are valid allowing 1 All functions of password 3000 3210 2 Display pulse graph of sample data 3 System software upgrade Shift F8 2 Disable functions of the expanded keys after the password set to 3333 1 Disable functions of the expanded keys 3333 2 Enable functions of the expanded keys after the password changes to 3210 Auto Hematology Analyzer Service Manual V1 1 11 1 Password and Upgrade Software 11 2 Upgrade System Software The system software has been set up in the hematology analyzer After turning on the instrument you can use this method to upgrade the system software in the Count screen In the process of upgrading program make sure not to power o
4. Parameter Units Type System Stats Palete Valve Test Prepare to Ship lij Error Message O Calibration Manual Calibraton Help Auto Calibration Shutdown Troubleshooting Chapter 2 Troubleshooting 2 1 Check Procedure Check the instrument according to the check procedure below Measurement operation check Was the quantity of each reagent sufficient for measurement Were the correct or recommended reagents used Refer to Check Items Was the operating temperature proper before Instrument Check Was the supplied main power voltage correct After turning on the instrument Did the instrument fail to generate an alarm NO DILUENT Refer to HARDWARE FAIL I gt Troubleshooting and Alarm Background noise measurement Did the instrument fail to generate an alarm Was the result within the specification Control measurement Did the instrument fail to generate an alarm Was the result reproducibility good for HGB RBC WBC PLT HCT Was the obtained data within the range END Auto Hematology Analyzer Service Manual V1 1 2 1 Troubleshooting 2 2 Check Items before Instrument Check Check items before Instrument check Sampled Whole Blood Handling Check Use the instrument and diluent under the following operating conditions Around instrument Diluent temperature 15 to 35 C temperature 15 to 30 C humidity 10 to 85 atmospheric pressure 860 to 1060hPa W
5. The CPU system is the core of the drive board Its task is to receive the data from the host and generate lattice messages after calculation using a specified algorithm These messages are then sent to the thermal head to be printed out The CPU system can simultaneously collect data from both thermal head and drive board and display data sent to the host 3 14 Auto Hematology Analyzer Service Manual V1 1 Hardware Power conversion The recorder requires the system to provide two voltages 12 V and 5 V The 5 V is directly driven by the logic and analog circuit of the drive board and the thermal head Its current is less than 150 mA The 12 V is converted into 8 V by the DC DC on the board to drive the thermal head and he motor The current required is determined by the printing content and ranges from 0 5 A to 2A Motor drive Asmall motor is used to control the paper movement at the thermal head The processor on the drive board uses two motor drives IC LB1843 V to control and drive the motor These two IC s use constant current to control and drive the motor Status detection To correctly and safely control and drive the thermal head and the motor the drive board must use the sensor inside the thermal head to detect the following signals the position of the chart paper if the paper is installed and if the temperature of the thermal head has exceeded the limit Key Test Points NO NAME LOCATION FUNCTION 1 12 V J
6. a 48V Monitor Module 120VAC 48V Monitor hl n d 12V Monitor I 12V Monitor L HGB Signal Module Temperature E ej pg Monitor Module Figure 3 3 Auto Hematology Analyzer Service Manual V1 1 3 7 Hardware WBC and RBC PLT AMP Module The circuits of three AMP channels WBC RBC and PLT are basically the same except difference on small points RBC and PLT channel uses the first two stages of AMP circuit and is separated from each other since the third stage The following description uses WBC channel as the example The input signal is AC signal from uV to mV grade Therefore using capacitors to filter direct current signal and connect the AMP channel at the preliminary stage The preliminary stage AMP uses U14 to amplify the signal At the end of this stage passive RC circuit is applied to realize high pass filter The second stage AMP uses U15 Its feedback loop is the same as the first stage It uses parallel connected capacitors to realize low pass filter The third stage AMP uses U10 Its feedback loop adopts electronic potentiometer U19 which is controlled by CPU board so that circuit of this stage can provide different AMP multiples and realize the adjustable program controlled gain Besides a voltage regulating tube is connected to the circuit of this stage to protect U19 from being damaged due to too high voltage A passive RC circuit is connected at the end
7. a FDD 2 33 i 5 Interface JP2 8 a JPI g JI J2 5 7 J22 J17 J21 J8 z Ji z o a z 8 5 ui ies me Pes 2 START P4 P7 P8 amp a key JI 2 58 he Power HGB Sensor J6 Supply Linear Board A nalog Signal Board JI Teansto time PI Temp Sensor J5 JA 33 Power Switch WBC RBC Auto Hematology Analyzer Service Manual V1 1 A 1 Appendix Hydraulic Diagram of BC 3000PLUS 8 7 N 2 Ma 3 SR 1 18 17 w Ti 6 Ja PE X S 9 10 A ti 15 12 16 14 2 Ay A 2 Auto Hematology Analyzer Service Manual V1 1 P N 3001 20 18543
8. v Rinse WBC bath 1 366 dilution in WBC bath v 50ul syringe aspirate 24 8ul blood dilution Rinse and drain RBC bath Dispense diluent prefill the RBC bath v 1 44274 dilution in RBC bath v RBC PLT Count v Rinse RBC bath v Zap the apertures K End D 2 Figure 4 4 Prediluted Count 4 6 Auto Hematology Analyzer Service Manual V1 1 4 4 4 Startup S Startup D Clean WBC bath Clean RBC bath Hydraulic System Create positive pressure in vacuum chamber Flush WBC and RBC apertures Clean WBC bath Clean RBC bath Restore the pressure to normal state in vacuum chamber Clean WBC bath Clean RBC bath Check background End Figure 4 5 Startup Auto Hematology Analyzer Service Manual V1 1 4 7 Hydraulic System 4 4 5 Flush Apertures GE Apertures Empty WBC bath Empty RBC bath Create positive pressure in vacuum chamber Flush WBC and RBC apertures v Clean WBC bath Clean RBC bath 4 8 Adjust pressure in vacuum chamber CO Figure 4 6 Flush Aperture A
9. 5 10 Auto Hematology Analyzer Service Manual V1 1 System Structure Figure 5 24 Figure 5 25 10 Press ENTER to close dialog and sample probe returns to ready position Auto Hematology Analyzer Service Manual V1 1 5 11 System Structure 5 1 3 Replace Sample Probe Wipe Block Avoid directly contacting with the patient blood ZEN samples 1 Push the switch and open the right side door of the machine Figure 5 26 2 Lift up the switch and open the front panel Figure 5 27 Auto Hematology Analyzer Service Manual V1 1 System Structure 3 Figure 5 28 shows the machine with the front panel opened Figure 5 28 4 In Setup Password screen enter the password 3000 A Count Maintenance Uy Sample Mode System Status Review Valve Test si fe Quality Control System Test s gt Setup Prepare to Ship s Error Message Z a Calibration e Help db Shutdown imenu Figure 5 29 In menu operation move the cursor to Service System Test item and press ENTER to access the System Test screen Press 1 1 to move the cursor to Fluctuating Motor Press ENTER key to pop up the dialog box Auto Hematology Analyzer Service Manual V1 1 System Structure System Test WAIT WHORE tem Resut tern Resit WECAD Staus 25m amp S0ul Motor RBC AD Status 10eni Motor PLT A Statu
10. C The temperature sensor is abnormal Background Abnormal R o Nm AID The count baths or apertures are dirty There are bubbles in the tubing system The reagents are polluted or exceed their Exp date There is clog or bubbles error when test the background WBC Clog mo ume 8 problem 9 The WBC aperture is clog or dirty Foreign object has clogged the WBC bath WEC reference count time is set up improperly Optical couplers on the volumetric metering board are damaged or the values of potentiometers are set improperly The liquid cannot flow in the tubing smoothly pressed bends or clogged by foreign object Inadequate reagent Can not form stable surface in WBC metering glass tube The tubing has leaks or the vacuum system has The sample has problem such as the type and proportion of anticoagulant is selected improperly or 2 28 Auto Hematology Analyzer Service Manual V1 1 Troubleshooting there is blood clots WBC Bubbles WEC reference count time is set up improperly Optical couplers on the volumetric metering board are damaged or the values of potentiometers are set improperly Inadequate reagent not form stable surface in WBC metering glass tube tubing has leaks or the vacuum system has problem RBC Clog FON gt RW The RBC aperture is clog or dirty Foreign object has clogged the RBC bath RBC reference count time is set up improperly Optic
11. Figure 5 70 2 Disconnect the ground wire and data cable Figure 5 71 7 Remove this Cover ea lt lt Disconnect the cable and shield wire Figure 5 70 Figure 5 71 3 Open the unit s front pane and screw off the joint and ground wire Figure 5 72 4 Disconnect the cable to keyboard then lift the front panel and take it out Figure 5 73 Lift the front panel and take it out Figure 5 72 Figure 5 73 5 Screw off the 7pcs screws that fix the TFT bracket disconnect the backlight cable Figure 5 74 6 Screw off the 4pcs screw caps that fix the TFT screen disconnect all cables Figure 5 75 Auto Hematology Analyzer Service Manual V1 1 5 33 System Structure screw off the four adi screw caps to remove the TFT screen Disconnect the background connecting cable Figure 5 74 Figure 5 75 7 If the TFT background with bad brightness or contrast after replace TFT screen please refer to followed picture to adjust Figure 5 76 amp Figure 5 77 TFT backlight adjustable resistor Fi TFT screen constrast g adjustable resistor On ips CPU board Figure 5 76 Figure 5 77 5 34 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 1 11 Replace Recorder Paper Open the door of the recorder Figure 5 78 Pull the bar up and insert the record paper into the recorder box Figure 5 79 Auto Hematology Analyzer Service Manual V1 1 5 35 System Structure P
12. Press 1 1 to move the cursor to Fluctuating Motor Press ENTER to pop up the dialog box 5 8 Auto Hematology Analyzer Service Manual V1 1 System Structure System Test WAT WHOLE p Ji tern T Resit tem Resut f WEC AD Status 1 f 2 5rri amp SOul Motor RBC AD Status Tmi Motor PLT A Status Rotatory Motor WBC AD INT 447 Po xetusong Motor RBC AD INT Prt PLT AD INT Vacuum WEC Trne s Pressure RBC Trne s Pressure WEC Aperture V Vacuum Filter RBC Aperture V et TITS select tem ENTER to test tem Figure 5 18 Figure 5 19 5 Press 1 to move the sample probe to the upper position WI E b J FA NA E Figure 5 20 6 Loosen the nut of the fixing screw of fixing the sample probe Auto Hematology Analyzer Service Manual V1 1 5 9 System Structure Figure 5 21 7 Pull off the tubing connected to the top end of the sample probe Pull the sample probe out Replace the sample probe ee 7 Figure 5 22 8 When installing the sample probe first connect the tubing and install the sample probe as 5 23 Insert the localizer of the sampler probe from the bottom end of the sample probe wipe block Hold the localizer to make it cling closely to the sample probe wipe block until the bottom end of the sample probe contacts the localizer Figure 5 23 9 Fasten the fixing screw of the sample probe
13. RTC provides calendar and clock WDT is designed to protect the system in case the software fails to function It will generate reset signal once the software fails to function Super I O provides external interfaces including one parallel port two serial ports one keyboard interface and one floppy disk drive interface A D amp I O Module A D8I O module use FPGA and CPLD as the core and include other peripheral circuits like FIFO ADC and I O The A D circuit can convert the analog signal pre processed by the analog signal board and then send it to CPU via FIFO CPU uses I O interfaces to manage interrupts control valves and pumps control the gain of analog signal board and zap function and HGB light Display Module The display module uses FPGA as the core and includes other peripheral circuits like SRAM display interface I O Display module is designed to display the information on the LCD screen Block Diagram of CPU Board 3 2 Auto Hematology Analyzer Service Manual V1 1 Hardware Figure 3 1 shows the block diagram of CPU board Conputer systemof CPU Board RTC WDT SDRAM Flash Superl O hi li i 1 v v v v CPU v v Dspl ay nodule AD collection I O port FPCA FPCA PLD AD amp I O Figure3 1 Computer System Computer system includes CPU U33 SDRAM U1 U2 F
14. The specified diluent and Use the correct reagent hemolysing reagent were not used Cyanide in the hemolysing Replace the hemolysing reagent with a new reagent has been dissolved by sunlight or heat reagent and tighten the cap of the reagent bottle The HGB 2 5 mL syringe exists bubbles Remove the bubbles from the syringe The light axis of the HGB LED is deviated Adjust the light position Replace the light Auto Hematology Analyzer Service Manual V1 1 Troubleshooting The light of HGB is aging Replace the HGB unit The diuent syringe exists bubbles Remove the bubbles from the syringe Faulty the Analog board Replace the analog board Poor Reproducibility for WBC Possible Cause Countermeasure The background data on WBC is high Refer to Background Noise Check of this chapter Dirty WBC measurement bath Clean the WBC bath with E Z cleanser or probe cleanser Dirty measuring tube Clean the tube below the WBC bath Dirty wipe block Clean the wipe block Replace a new wipe block The sample probe position is not correct Adjust the probe position using localizer The 3 way valve SV12 cannot drain liquid empty Replace the 3 way valve SV12 The 3 way valve SV12 is dirty Clean the valves Replace this valve The lyse reagent has been dissolved by sunlight or heat Replace the lyse reagent with a new re
15. This is because the RBC membrane s resistance against hemolysing 2 2 Auto Hematology Analyzer Service Manual V1 1 Capillary Blood Handing Check Troubleshooting reagent is increased insufficient hemolysing and it will cause an increase of the WBC count when the blood is measured with the hematology analyzer Furthermore the bilirubin and WBC in the blood may affect the hemoglobin concentration in the measurement A cautionA In the capillary blood mode the instrument aspirates the diluted sample of 20uL In this mode if the venous blood is incorrectly aspirated instead of capillary blood there is a high possibility that the fluid path is clogged or the background noise is not easily decreased Most causes of data error using capillary blood are due to incorrect technique for the capillary blood sampling and diluting Therefore take care the following notes and make a capillary blood sample A noTeA Dilute the sampled capillary blood correctly the first time because it is difficult to sample the blood twice from the capillary Auto Hematology Analyzer Service Manual V1 1 2 3 Troubleshooting 2 3 How to Check Sample Data Background Noise Check 2 4 This check is used to make sure that the counted and calculated data of a diluent sample is not affected by background noise If the background checking value exceeds the tolerable dilute data shown in the table below the diluent data counted and
16. alarm It is forbidden to adjust the vacuum value discretionarily in order to meet the reguirement for count time of WBC and RBC The reference count time should be reset in the following status 1 The volumetric metering board or the volumetric glass tube has been changed 2 The count baths or the apertures have been changed 3 The analog signal board has been changed or the system vacuum has been adjusted 4 The system vacuums are excursion because the parts are aging Set up the reference count time 1 Confirm that vacuum error does not occur 2 Execute Probe Cleanser Cleaning in Service Maintenance 3 Execute 6 blank counts and write down the WBC count time and RBC count time of each time 4 Calculate the average time of the 6 WBC counts and RBC counts Enter 3000 in the Setup Password screen 6 14 Auto Hematology Analyzer Service Manual V1 1 Adjustment 6 Set the WBC count time and RBC count time in the Setup Count Time screen to the average time above 7 Enter 0000 in the Setup Password screen Auto Hematology Analyzer Service Manual V1 1 6 15 Adjustment 6 10 Adjust Auto Clean Time The Hematology analyzer should be cleaned after measure 4 hours or 50 samples it s better for maintenance and making the equipment under good working condition 1 Refer to Figure 6 13 amp Figure 6 14 input password 3000 2 Move the cursor to Auto Clean Time item pr
17. Baths Flush Apertures Emety Tung Probe Cleanser Cleaning Wipe Block Clearing Emety WSC and RBC baths and add Ottuert Buen Press A T Elio select tem Torun Figure 5 64 TT Maintenance nas i Diluent Prime E Z Cleanser Cleaning Rinse Prime Lyse Test Lyse Pome Clean Baths 67 EEEN Empty Tubing Zap Apertures Plush Apertures Probe Cleanser Clearing Wipe Block Ciraning Emptying WSC and RBC baths m mio o o PI Figure 5 65 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 1 9 Clean V11 or V12 1 Remove the valve from the instrument as above 2 Use T9 screwdriver in kit box to unscrew the four fixing screws on the valve bonnet open the bonnet note the position and direction of NO valve port 3 Use distilled water to clean the valve membrane inside the bonnet do not let liquid flow into the valve seat 4 Dispose the foreign object in the valve then mount the valve bonnet Use T9 screwdriver to fasten the screws Valve bonnet Screw Valve seat Figure 5 66 7 F Valve bonnet Valve seat Figure 5 67 Auto Hematology Analyzer Service Manual V1 1 5 31 System Structure ice Valve membrane Figure 5 68 Screw C port NO port Figure 5 69 5 32 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 1 10 Replace TFT Screen 1 Open the left side door then screw off the 3pcs screws on the shield cover
18. Explanation 1 GND 12Vp power Ground 2 PGND 12Vp power Ground 3 PGND 12Vp power Ground 4 12VP 12 VP 5 12VP 12 VP 6 12VP 12 VP J12 30V Connector PIN Defined Explanation 1 30GPGND 30V power Ground 2 30GPGND 30V power Ground 3 30VP 30VP 4 30VP 30VP Auto Hematology Analyzer Service Manual V1 1 System Structure 5 4 3 Analog Board s connectors defined J1 Connect to CPU Board PIN defined Explanation 1 AWBC WBC Signal 2 12VP MON II 12VP Monitor signal 3 ARBC RBC Signal 4 DVCC MON DVCC Monitor signal 5 APLT PLT Signal 6 AGND AGND 7 AGND AGND 8 12VA 12VA ADC 9 AGND AGND 10 12VA 12VA ADC J2 A D Control Signal PIN Defined Explanation 1 V5P0 5V DDC 2 GND Digital Ground 3 XCHS4 4 DGAIN2 5 XCHS3 6 DGAIN1 7 XCHS2 8 DGAINO 9 XCHS1 10 DBURNO Zap Control 0 11 XCHSO 12 DBURN1 Zap Control 1 13 HGB LIGHT1 HGB light Control 14 DCONSTO Constant Current control J38J4 RBC 8 WBC Sensors J5 TEMP Sensor connector PIN Defined Explanation 1 RT ENVIR1 2 RT ENVIR2 3 RT REAGENT1 4 RT REAGENT2 J6 HGB Connector PIN Defined Explanation 1 GND AGND Auto Hematology Analyzer Service Manual V1 1 System Structure 2 HGB Constant Current 3 GND AGND 4 HGB IN HGB Signal 5
19. General Chapter 1 General 1 1 Introduction A cauTIonA To maintain the instrument in normal condition the user must perform the periodic maintenance Refer to the user manual This service manual provides useful information to help service personnel to understand troubleshoot service maintain and repair the Hematology Analyzer All replaceable parts or units of this instrument and its optional units are clearly list with exploded illustration to help you locate the parts quickly The maintenance must be periodically performed because the instrument has fluid paths and precision parts Accordingly the user is responsible for performing the periodic maintenance The maintenance chapter in this service manual describes the maintenance that should be performed by the qualified service personnel The maintenance chapter in the user manual describes the maintenance that can be performed by the user A noTeA If the instrument has a problem and there has been no periodic maintenance the instrument will usually be normal again by cleaning the fluid paths or replacing a consumable with a new one The information in the user manual is primarily for the user However it is important for service personnel to thoroughly read the user manual and service manual before starting to troubleshoot service maintain or repair this instrument This is because service personnel needs to understand the operation of the instrument
20. HGBt Constant Current J7 AC Power Connecter PIN Defined Explanation 1 120VAC2 120VAC 2 120VAC1 120VAC 3 53VAC2 53VAC 4 53VAC1 53VAC J8 5V Power PIN Defined Explanation 1 5VGND 5VGND 2 5VGND 5VGND 3 5VGND 5VGND 4 5VPOW 5V 5 5VPOW 5V Auto Hematology Analyzer Service Manual V1 1 System Structure 5 4 4 Keyboard Connectors defined J1 Connect to CPU board PIN Defined Explanation 1 7 10 16 Keyboard Scan signal input 8 7 Buzzer control signal 9 12 00 03 Keyboard Scan signal input 13 5V 5V 14 DGND DGND 15 DGND DGND 16 PGND Power GND 17 PGND Power GND 18 12V 12V 19 12V 12V 20 LCDBCTL LCD backlight brightness control J2 LCD backlight brightness control PIN Defined Explanation 1 12V 12V 2 PGND PGND 3 CTL Brightness Control 4 VBACK LCD Brightness adjust 5 NC J3 Connect to Indicator Board PIN Defined Explanation 1 DGND DGND 2 5V 5V 3 LCDBCTL LCD Brightness control Signal 4 MCUBCTL LCD Brightness control Signal J4 Contrast Adjustment Connector PIN Defined Explanation 1 DGND DGND 2 Vcon Contrast Adjust 5 54 Auto Hematology Analyzer Service Manual V1 1 5 4 5 Indicator Board Connector Defined System Structure
21. Light Show hematology analyzer work status 5 Start key Press to aspirate the sample and start counting 6 Sample Probe Aspirate the sample Dispenses the diluent when in capillary blood mode Keypad 1 START In Count screen QC Count screen and Auto Calibration screen press it to count In the status of Adding Diluent press it to add Diluent 2 MENU Press this key to switch between window operation and menu operation 3 PRINT Press this key to print using either recorder or printer 4 FEED Press this key to feed paper of the recorder Release it to stop the operation 5 MUTE Mute the alarm and clear some of the error messages 6 DEL Delete the selected data in Review screen Delete error message in Error Message screen Delete reference data and running control data in QC Edit screen Call default value in Normal Range screen 7 0 9 Enter numbers Auto Hematology Analyzer Service Manual V1 1 1 7 General 8 T I I Move the cursor in the window area or menu area 9 ID Enter the ID number of the sample 10 DILUENT In the Count screen of Prediluted mode press this key to enter the Adding Diluent status 11 PgUp PgDn Scroll the screen up or down page by page 12 ENTER Confirm 13 STARTUP Clean the tubing baths and sample probe then check the background 14 FLUSH Press the key to execute the Flush operation to remove the clogs 1 8 Auto Hematology Analyz
22. Printer Power PIN defined Explanation 1 12VP 12V 2 PGND Power GND 3 Vprn Printer Power 4 12VP 12V 5 PGND Power GND 6 Vprn Printer Power J14 HD Connector 5 44 Auto Hematology Analyzer Service Manual V1 1 J15 HD Power Supply System Structure PIN defined Explanation 1 12VP 12V 2 PGND Power GND 3 V5P0 5V 4 GND Digital GND J16 PIN defined Explanation 1 DGND Digital GND 2 LQ1 3 DGND Digital GND 4 LQ2 5 DGND Digital GND 6 LQ3 7 DGND Digital GND 8 LQ5 J17 A D Input PIN defined Explanation 1 AWBC WBC Signal 2 12VP MON II 12VP Monitor signal 3 ARBC RBC Signal 4 DVCC MON DVCC Monitor signal 5 APLT PLT Signal 6 AGND AGND 7 AGND AGND 8 12VA O 12VA ADC 9 AGND AGND 10 12VA 12VA ADC J18 MTB Board connector PIN defined Explanation 1 V5P0 5V 2 START 1 WBC Start 3 GND Digital GND 4 STOP1 WBC Stop 5 NC NC 6 START2 RBC Start 7 PGND Power GND 8 STOP2 RBC Stop 9 12VP 12V 10 HGB_LIGHT HGB Light control Auto Hematology Analyzer Service Manual V1 1 System Structure J19 BDM Connector PIN Defined Explanation 1 NC 2 BKPT 3 GND Digital Ground 4 DSCLK 5 GND Digital Ground 6 NC 7 HR 8 DSI 9 JU3 2 3 3V 5V
23. The power supply board is abnormal The analog signal board is abnormal 48V Power Error The power supply part is abnormal The analog signal board is abnormal WBC A D Error The CPU board is abnormal RBC A D Error The CPU board is abnormal PLT A D Error The CPU board is abnormal WBC Interrupt Error a N ID ID jo The CPU board is abnormal Auto Hematology Analyzer Service Manual V1 1 Troubleshooting RBC Interrupt Error The CPU board is abnormal PLT Interrupt Error The CPU board is abnormal HGB Error The HGB LED loosens The HGB unit is bedabbled The HGB LED is damaged WBC bath is dirty Diluent is polluted or exceeds its Exp date HGB Adjust The HGB LED loosens The HGB unit is bedabbled Light intensity of the HGB LED is set incorrectly WBC bath is dirty Diluent is polluted or exceeds its Exp date Vacuum Filter Error The filter is clogged by dirt Vacuum Low WON SF FIA PON R O BRON S o A Vacuum pump is damaged Tubing or vacuum chamber has leaks Tubing connecting vacuum chamber to sensor loosens or falls off The valve connecting the vacuum chamber is damaged The waste container is placed over normal position or the waste tubing is too thin or too long or the waste tubing cannot drain the liquid smoothly Envir Temp Abnormal The environment temperature is over the range 15 35
24. Tube Auto Hematology Analyzer Service Manual V1 1 4 19 System Structure Chapter 5 System Structure 5 1 Disassemble Replace Parts and Components 5 1 1 Disassemble Syringe and Replace Piston AA ZN waRNING N Potential biohazard It is unnecessary to remove the syringe assembly from the instrument Push the switch as shown in figure 5 1 and open the right side door of the machine Figure 5 1 Figures 5 2 through 5 8 show how to remove the 10ml syringe and replace the piston 1 Take out the screw on the baffle Figure 5 2 2 Remove the baffle Auto Hematology Analyzer Service Manual V1 1 5 1 System Structure Figure 5 3 3 Take out the fixing screw between the 10ml motor lever and white metal connector Figure 5 4 4 Rotate the step motor lever out from the metal connector Figure 5 5 5 Remove the syringe and metal connector 5 2 Auto Hematology Analyzer Service Manual V1 1 System Structure Figure 5 6 6 Remove the bottom of the syringe Figure 5 7 7 Replace the piston s Figure 5 8 Install the syringe by following the above steps in reserve order Auto Hematology Analyzer Service Manual V1 1 5 3 System Structure If to replace the syringe you need to rotate the original syringe out from the metal connector Figures 5 9 through 5 13 show how to remove the 2 5ml syringe and replace the piston 1 Take out the
25. assay sheet Run the control again replace a new control to try again if the results are out of range After that if the result are still unacceptable recalibrate the instrument with the following procedures 5 When the condition temperaare range is out of 20 to 26 C control results maybe out of limits Auto Hematology Analyzer Service Manual V1 1 Troubleshooting Parameter Data Check with Hematology Control Check that the obtained sample data for each parameter is within the range of the assay values on the assay sheet A notesA If the data for any parameter is out of range calibrate the instrument according to Calibration of the operator s manual To calibrate the instrument for more accuracy refer to the following Procedure for Instrument Fine Calibration of this chapter Procedure for Instrument Fine Calibration The instrument allows the user to input the factors manually with the range between 75 and 125 The procedures of manual are 1 Confirm the sample mode 2 Run the calibrator in Count screen for at least five times The reproducibility of WBC RBC HGB MCV and PLT must satisfy following limits Parameter CV WBC lt 2 5 RBC lt 2 0 HGB lt 1 5 MCV lt 0 5 PLT lt 5 3 Calculate the new calibration factors 4 Enter the new calibration factors Calculate the New Calibration Factors Use the below formula to calculate the new calibration fac
26. because WBC count result is less than a certain value WBC lt 0 5 Auto Hematology Analyzer Service Manual V1 1 10 1 Histograms and Pulse Graphs 0 50 100 150 200 250 300 fL WBC 0 4 x 1097 Figure 10 3 4 No differential result because the peak of WBC histogram lies in the middle of the histogram and thus cannot identify the type of peak cells 0 50 100 150 200 250 300 fL Figure 10 4 5 Increased nucleated erythrocytes or interference or inadequate hemolysis 0 50 100 150 200 250 300 fL Figure 10 5 6 Severe interference in WBC channel identifying if it is interfered by observing the pulse graph 0 50 100 150 200 250 300 fL Figure 10 6 7 No lyse reagent or poor hemolysis 10 2 Auto Hematology Analyzer Service Manual V1 1 Histograms and Pulse Graphs O 50 100 150 200 250 300 fL Figure 10 7 8 Increased neutrophilic granulocytes Yo NN O 50 100 150 200 250 3001 Figure 10 8 9 Increased lymphocytes O 50 100 150 200 250 3008 Figure 10 9 10 Tumor patient R4 EN O 50 100 150 200 250 3001 Figure 10 10 11 Increased mid sized cells 0 50 100 150 200 250 300 fl Figure 10 11 Auto Hematology Analyzer Service Manual V1 1 10 3 Histograms and Pulse Graphs 10 2 Pulse Graphs After each count the system can save the original sampling pulses of this time We can analyze the reason leading to the fault by viewing these original data Enter passwo
27. calculated before background noise is reduced erroneous In the table below each diluent data is defined as follows Recommended diluent data This data is best for acquiring accurate data of the sample Acceptable dilunt data This data is the minimum value for acquiring accurate data of the sample Recommended diluent Data Acceptable diluent Data WBC 0 0 WBC 0 3x10 RBC 0 00 RBC 0 03x10 7 HGB 0 HGB 1g L or 0 1g dL HCT 0 0 HCT 0 5 PLT 3 PLT 10x10 Refer to Troubleshooting Erroneous Data of this chapter for the possible causes of background noise and how to reduce it Check Procedure 1 Press the start key to count and calculate the diluent There is no need to aspirate the diluent from the sampling probe 2 Make sure the counted and calculated data is less than or equal to the acceptable diluent data as shown upper If they are out of range decrease the background noise Parameter Data Check with Diluent Check that the background values are less than or equal to the data in the previous table Discard the other parameter values because they are not affected by noise Especially check the data for the PLT parameters When the diluent includes the particles of dust smaller Auto Hematology Analyzer Service Manual V1 1 Troubleshooting than WBC and RBC parameters the data of them is not affected by the dust but the data for the PLT parameter increases because the volum
28. of 5 25mA CPU board generates signal to control the optical couplers This signal is isolated from Q1 by an optical coupler e Circuit for Detecting Signal of Optical Couplers The detecting signal is output from the receive terminal of the optical coupler Use potentiometer to adjust the output voltage so that the output voltage of the detecting signal of the optical coupler can be 2 5V Circuit for Outputting Comparative Signal Connect U11 with the comparator to form hysteresis comparator with the hysteresis difference set to 0 25V Moreover a LED is added into this part to serve as the indicator LED lighting on means the optical coupler has detected liquid Auto Hematology Analyzer Service Manual V1 1 3 17 Hardware 3 7 Power Supply Board General This power supply is designed for use only on BC 3000PLUS hematology analyzer There are two types of power supply board one input power is AC220V 20 and another input power is AC110V 20 The outputs of rating load are 5V amp 3Amax 12V amp 3Amax and 432V81A The outputs of maximum load are 5V amp 7Amax 12V amp 6 5Amax and 32V amp 3A peak The outputs of the three ways are isolated from each other in order to reduce the noise interference Working Principle The two types power supply boards have the same working principle The power supply board filters and rectifies the input AC supply in order to obtain a smooth DC voltage with the amplitude about 1 2 times of the i
29. screw on the baffle Figure 5 9 2 Then take out the fixing screw Figure 5 10 3 Remove the syringe 5 4 Auto Hematology Analyzer Service Manual V1 1 System Structure SY Figure 5 11 4 Remove the bottom of the syringe Figure 5 12 5 Replace the piston Figure 5 13 Install the syringe by following the above steps in reserve order Auto Hematology Analyzer Service Manual V1 1 5 5 System Structure Replace the piston of 50ul syringe in the same way as that for replacing the piston of 2 5ml syringe 5 6 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 1 2 Replace Sample Probe 1 Push the switch and open the right side door of the machine 9777 Figure 5 14 2 Lift up the switch and open the front panel Figure 5 15 3 Figure 5 16 shows the machine with the front panel opened Auto Hematology Analyzer Service Manual V1 1 5 7 System Structure Figure 5 16 4 In Setup Password screen enter the password 3000 A Count Maintenance Ul Sample Mode System Status AF Calibration e Help ch Shutdown E Review Valve Test a fe Quality Control PASTE GUMEE i CF Setup Prepare to Ship E Rh Service Ad Error Message a Ld imenu Figure 5 17 In menu operation move the cursor to Service System Test item and press ENTER to access the System Test screen
30. the RBC bath RBC always clog 1 RBC bath is dirty 2 The count time is a little longer than the setting time 3 One of the sensor which on the MTB board is broken 1 Clean the bath using E Z cleanser or probe cleanser 2 Re set the count time 3 Replace the MTB board RBC bath is leakage RBC bath is broken with Replace the RBC bath cranny RBC background and value is RBC bath is broken with Replace the RBC bath too high cranny RBC no result Analog board is broken Replace board the analog S Stability is bad and PLT over 1000 sometime There are some bubbles in Diluent syringe and make the injected diluent not enough Replace the SV3 valve Sample probe leakage SV4 valve is leakage Replace the SV4 valve Show 8002 error code The disk on module is broken Replace the disk on module Show 48V is low The transformer or analog Replace transformer Auto Hematology Analyzer Service Manual V1 1 2 25 Troubleshooting board is broken or analog board T TEMP is low The environment Increase the temperature is low environment TEMP V Vacuum is low Air filters are so dirty Waste liquid tube is kink Tube is leakage Vacuum pump is leakage Replace the filters Loose the tube Replace one new tube Replace a new pump Vacuum chamber is leakage Replace
31. the sample probe wipe block Hold the localizer to make it cling closely to the sample probe wipe block until the bottom end of the sample probe contacts the localizer Figure 5 36 11 Fasten the fixing screw of the sample probe Figure 5 37 5 16 Auto Hematology Analyzer Service Manual V1 1 System Structure Po ge Figure 5 38 12 Press ENTER to close dialog and sample probe returns to ready position Auto Hematology Analyzer Service Manual V1 1 5 17 System Structure 5 1 4 Replace Vacuum Chamber ZN WARNING Potential biohazard Pull off the tubing connected to the outlet of the vacuum chamber Remove the fixing screw of the vacuum chamber so as to remove the clip washer and vacuum chamber in turn see figures 5 39 through 5 40 Then install the chamber by following the above steps in reserve order Figures 5 40 5 18 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 1 5 Replace Pump ZN WARNING Potential biohazard 1 Pull off the tubing connected to the corresponding pumps Remove the two screws used to fixing the bracket Figures 5 41 Figures 5 42 2 Use hand to tilt the bracket and the pump outward as shown in figures If to replace Auto Hematology Analyzer Service Manual V1 1 5 19 System Structure the pressure pump just cut off the white tie around the pump then remove and replace the pump If to replace the
32. three modules which U21 controls the rotatory and fluctuating motor in order to control the sample probe assembly The rotatory motor uses constant voltage full step driver way while the fluctuating motor adopts constant current half step driver way In addition U26 and U27 are used to control the motors of 10ml syringe and 2 5ml amp 50ul syringe by means of constant current half step drive The driving circuit of rotatory motor uses optical coupler isolation and is driven by U9 U15 acts to drive the fluctuating motor and syringe motors Its output can be 2A per phase Current limiting circuit and voltage protective circuit are realized respectively by L6506D and UC3610 The setup limit current is 1A The driving voltage of motor shouldn t exceed 50V Power Supply Provide 5V for power board U29 is used to realize the constant voltage output and Q2 for amplifying the current The maximum current that can be provided is 4 5A The internal control supply of the power board is realized by LM7805 Auto Hematology Analyzer Service Manual V1 1 3 4 Keypad Functions and Modules Hardware The keypad circuit may not only support the input via man machine interface but also control the buzzer and LCD backlight The main circuit modules are 1 Key matrix the key matrix circuit IO 16 OOQ 03 make up of 7x4 key matrix There are totally 23 keys on the keypad When single key is pressed only a unique pair between 10 16 and O0 O3
33. unique Variable resistance on the CPU board You can adjust it to modify the display brightness real time 6 12 Auto Hematology Analyzer Service Manual V1 1 Adjustment 6 8 Adjust Vacuum and Pressure VR1 controls vacuum and VR2 controls reference pressure atmospheric pressure VR5 controls pressure and VR6 controls reference pressure atmospheric pressure Vacuum should be at least lower 22Kpa than atmospheric pressure Auto Hematology Analyzer Service Manual V1 1 6 13 Adjustment 6 9 Adjust Count time Count time is indicating the time of that liquid surface inside the glass tube of the volumetric unit flow from the upper optical coupler to the lower optical coupler Under a certain vacuum the count time is decided by the following factors 1 Diameter of the aperture 2 Thickness of the aperture 3 Inside diameter of the glass tube 4 Length between the upper and lower optical couplers For HEMATOLOGY ANALYZER when the vacuum negative pressure is 230mBar the count time of WBC is between 11 5 and 15 seconds and the flow time of RBC is between 14 and 19 seconds After each count the system will compare the count time of WBC and RBC with the reference count time pre defined in the system If the real count time is 2 seconds longer than the reference count time the system will give Clog alarm If the real count time is 2 seconds shorter than the reference count time the system will give Bubbles
34. 0 valve 5 22 valve 7 Auto Hematology Analyzer Service Manual V1 1 General 1 4 5 Left side view without the door 4 1 Mi Figure 1 5 1 hard disk Module on disk 2 vacuum pump 3 pressure pump 4 pressure chamber Auto Hematology Analyzer Service Manual V1 1 1 13 General 1 5 Menu Structure Chart ie L included items not sub menu items can be viewed Only after input the corresponding password Auto Hematology Analyzer Service Manual V1 1 Count Ls Whale Blood Review Sample Review FA E E E TT Sample Histogram Review Quality Control gt Commerical QC Edit HN File 1 Control OCComt File 1 Hie QC Graph Ee mes C OCT J EL Bile ES Fite X B Analysis 1 limit File 2 Hle j Me Tes l Sample Batch File 3 File 4 File 5 File6 Star Stop ere Es O Hey emcee EE ED es Deeg poo ED ee s n pes r Count Time se Print p o File 9 Select Password General Pant moar RBC Comt Time NE _Date 6 Time CM Mid Mix Wim Gan Neonate Version Auto Clean Time Reagent Exp Date Language Print Caption
35. 0 5 HGB s0 5 PLT lt 1 Linearity Run the test in prediluted mode Select one extra high concentration sample each for WBC RBC PLT and HGB Individually analyze WBC RBC PLT and HGB according to the linear concentrations of 100 80 60 40 20 and 10 Analyze the sample of each concentration for 2 times Calculate the average value and use it as the result Respectively make the linear regression analysis of WBC JRBC JPLT and HGB using two way analysis of variance Linearity Limits 9 2 Difference Parameter Linearity range Unit f whichever is greater WBC 0 3 0 99 9 x10 L 0 3 or 5 RBC 0 20 D 9 99 x10 2 L 0 05 or 45 HGB o O 300 g L 2 or 3 PLT 10 999 x10 L 10 or 10 Auto Hematology Analyzer Service Manual V1 1 Histograms and Pulse Graphs Chapter 10 Histograms and Pulse Graphs 10 1 Histograms This section demonstrates some usual WBC histograms 1 Normal histogram O 50 100 150 200 250 300 fL Figure 10 1 A noTeA Blood cells lain between the first and the second discriminators are lymphocyte those between the second and the third discriminators are mid sized cells those between the third and the fourth discriminators are granulocyte The fourth discriminator is the fixed line 2 No differential result because the WBC histogram is over narrowly compressed 0 50 100 150 200 250 300 fL Figure 10 2 3 No differential result
36. 0ml syringe dispenses diluent into the RBC bath to prefill it The 2 5ml syringe sends 0 5ml lyse reagent to the WBC bath for a final 1 308 dilution while the 10ml and 50ul syringes dispense 15 6ul of the 1 269 dilution and additional diluent into the RBC bath for a final RBC PLT dilution of 1 44833 Mixing bubbles enter the baths to mix the bath contents The vacuum chamber drains Both dilutions WBC and RBC PLT are drawn through the apertures via regulated vacuum The instrument counts 500ul dilution for WBCs and counts 300ul dilution for RBCs and PLTs After counting finishes the flow ends The system takes an HGB sample reading The system analyzes the data while the WBC and RBC baths drains and rinses The system zaps the apertures and the probe moves to the aspirating position The system displays results on the screen The system is ready for the next sample Auto Hematology Analyzer Service Manual V1 1 4 3 Hydraulic System 4 4 Flow Charts of Main Procedures 4 4 1 Power on Abnormal nformation about last shutdown Startup sequence of abnormal shutdown Startup sequence of normal shutdown Startup sequence after empty tubing v v v Background Check Pass Count time lt 2 Y Ready to count Abnormal background error Ready to count Clog Clog moving sequence Bubbles Cleaning sequence
37. 1 Histograms and Pulse Graphs whe length 2379108 current ponton 2376704 error Trees to ov nder m gt 80 serol page TILI to scrol 30 pages ENTER Vo ext Figure 10 34 Interference in RBC channel from tubing Feature data length increases the base line of signal is not stabile turrent porton 1152000 Trees Z to show nder Figure 10 35 e Insufficient liquid in RBC bath during count the length 163886 currert poston 179712 A Prose E to show rater A to serol 1 page 11 4 10 serol 50 pages ENTER to ext Figure 10 36 e Interference in PLT channel from tubing 10 12 Auto Hematology Analyzer Service Manual V1 1 Histograms and Pulse Graphs Feature data length increases the base line of signal is not stabile p length 2048000 current postion 1510400 Wry A a na na udi Ro OAZA pel hay ZNE 4 Presa 3 vo show ruler fee to scroll 1 page 111 to scrot 0 pages ENTER to ent Figure 10 37 e Insufficient liquid in RBC bath during count ph kagi 239929 arent portion 204800 4 a Hi le OVI fji to sarot 1 page TY1 to sarot 30 pages ENTER to ezt Figure 10 38 Interference in WBC channel from tubing Feature data length increases the base line of signal is not stabile pit lengi 2221232 Curent porton 91920 Prose 8 to dam ruler es Ee 40 pero pega TU to ser 30 panes
38. 10 Replace TET Seren ives iccs 2cccyedeceeisdide cupuseshandededbesesoaethendeiecueshdseethdededaciwangagts 5 33 b l 14 lt Repla e Recorder Papeleria i ees 5 35 5 1 12 Replace Recorder Module nn 5 37 5 2 Disassemble assemble Circuit Boards cececcceeeseeeeeeeeeeeeeeseneeeeeeeneaeeeeenaeeeeee 5 38 5 3 Connect Power Supply e szeszes sie Banat ed nee ices ei 5 39 5 4 Connect Circuit BOardS arer saa a feeder tees a tented nineteen 5 41 5 4 1 CPU board connectors defined 5 43 5 4 2 Power Driver Board Connectors Defined eceeeeeesneeeeeneeeeeeeneaeeeeeeeneeeeeeaas 5 48 5 4 3 Analog Board s connectors defined 5 52 5 4 4 Keyboard Connectors defined 5 54 5 4 5 Indicator Board Connector Defined 00 0 0 ee eeceeeeeee ene eeeeeeeeeeeeeeeeeeeeeneaeeeessaaaees 5 55 5 4 6 MTB Connector Defined eeeeeceeeeseececeeesneceeneeeeceeeeeaeeenecsaneeneseseaeereesaseners 5 55 Chapter 6 Adjustment sis sce ecssceeee secs ceveterseatarsateeteuds st eko tweens sveduenwenscebateuseween nes 6 1 6 1 General nire aare kezdenek VRE bend dies enn eee dini 6 1 B Z Adjusted proc dure Sacin AI ej veka ORA Mesa aa aden dhe tua 6 1 6 3 Gain of WBC whole blood and prediluted Channel 6 4 6 4 Gainof RBC Channel Sakini a ein ine ea eR 6 6 6 5 Gain of PLT Channel si ccicncni nisi dasi nei s narr A legi eleszi ein szd sien et 6 10 6 6 Gain of HGB Channel 6 11 6 7 Adjust Displa
39. 2 3 2003404 mev 100 100 0 200204 KEZI se Figure 6 17 Select Yes the system will save the new calibrating factor 3 Use control data to confirm gain In whole blood mode count twice in the Count screen by using control Calculate the average value of MCV M RBC factor RAN a RT ei leti 1 MCV measured value As shown in figure 6 4 press 1 to move the cursor onto RBC option press lt to modify the factor to make it the same as the calculated result of formula 1 Auto Hematology Analyzer Service Manual V1 1 6 9 Adjustment 6 5 Gain of PLT Channel After confirming the gain of RBC channel set up the gain of PLT channel As shown in figure 6 4 press f to move the cursor to PLT item press d to modify the current value of PLT to make it the same as the current value of RBC 6 10 Auto Hematology Analyzer Service Manual V1 1 Adjustment 6 6 Gain of HGB Channel 1 As shown in figure 6 4 press t to move the cursor to HGB item press to modify the background voltage value of HGB within the range of 4 3 4 5V 2 Adjust the VR4 and VR3 that on the analog board to adjust HGB background voltage VR4 controls HGB base point voltage VR3 controls HGB background voltage Auto Hematology Analyzer Service Manual V1 1 6 11 Adjustment 6 7 Adjust Display Brightness Remove the top cover of the Hematology analyzer there is a
40. 3 1 44274 Cell Counting Aperture Size WBC 100um RBC 70um Throughput more than 60 samples hour Check Diluent Rinse and Lyse The applied volume of each reagent is Diluent Rinse Lyse E Z Normal Startup 42ml 10ml Prepare a sample 25 4ml 6ml 0 5ml whole blood Prepare a sample 25 1ml 6ml 0 26ml prediluted Normal Shutdown 32ml 10ml 1 6ml Performance Specifications Imprecision General Imprecision is based on replicate determinations of the same sample The first Auto Hematology Analyzer Service Manual V1 1 1 3 General result is not used in the calculation Imprecision Specifications Parameter Level Units CV WBC 7 0 15 0 10 7 L lt 25 RBC 3 5 6 0 10 L lt 2 HGB 110 180 g L lt 15 MCV 80 0 110 0 fL lt 0 5 PLT 200 500 109 L lt s 5 Operating Range Parameter Range Units WBC 0 0 999 9 10 L RBC 0 00 9 99 10 L HGB 0 300 g L MCV 0 250 fL PLT 0 3000 10 L Linearity Parameter Linearity Range Units i A whichever is greater WBC 0 3 99 9 10 L 0 3 or 45 RBC 0 20 9 99 10 L 0 05 or 45 HGB 0 300 g L 2 or 13 PLT 10 999 109 L 10 or 10 Display Liquid Crystal Display LCD resolution 640 x 480 Input Output Two RS232 C serial ports One printer port One keyboard interface Built in Thermal Recorder Auto Hematology Analyzer Service Manual V1 1 General Printer optional E
41. A Mite ad szek eg szt eee 2 21 A SI N E AAA EEE EPE A NE EO EOR ENE EEA EPO PER RE ZN 2 27 Chapter 3 Hardware szeszes ses kek EN A S SE EIN ES SAPE SLA sil 3 1 9 1 CPU Boatdii ct ns ai je ai ie avi a el ni A bk deveti Melika 3 2 3 2 Analog Signal BOATA e a ana ae ae Ae tees alate a dla Wate Ehre u DA 3 6 9 9 Powel DIVO Boal isis iii rare SA reki eri been be Mrb ave i 3 10 3 4 Key Pad ies cient odje neni AW adil iva ea dedov e share 3 13 3 5 Recorder B ards sss a e ek ee liki kel JE Pad uct ves Ak Elek stak 3 14 3 6 Volumetric Metering Board 3 16 3 POWer Supply BOALA is sava ei titer ate ete ee 3 18 Chapter 4 Hydraulic System ee 4 1 4 1 Hydraulic System Block Diagram 4 1 4 2 Units of Hy drauliG Sy SE e r para eden V ete eet eee Beaded 4 2 4 3 Whole Blood Count Cycle 4 3 4 4 Flow Charts of Main Procedures nana 4 4 4 4 1 POWEONI iii za ei HR MA lai eki ii iji 4 4 4 42 Whole Blood COUNT ss sane dije aed eee AA eki 4 5 4 4 3 Predilut d GO T szeszet seine A odi s T SE E a a keszeg ve di dovina deine 4 6 4 44 Stal lunin eli pivi eed ebe ge even eki le Eaa EE REEE 4 7 474 5 m AWAN OTETA EE sn NA RE E E en eats 4 8 4 4 6 DISPENSE DilUONIt epe e a a a a ar neee z legye 4 9 4 47 Shut DOWN sss en jeva lech daa i a Ve bebe vine ta 4 10 Auto Hematology Analyzer Service Manual V1 1 1 Content 4 5 Hydraulic System Flow Diagram ne 4 11 4 5 1 Inspire Sample and Dilu
42. BC 3000 Plus Auto Hematology Analyzer Service Manual Copyright SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD 2003 Statement SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD hereinafter called Mindray owns all rights to this unpublished work and intends to maintain this work as confidential Mindray may also seek to maintain this work as an unpublished copyright This publication is to be used solely for the purposes of reference operation maintenance or repair of Mindray equipment No part of this can be disseminated for other purposes In the event of inadvertent or deliberate publication Mindray intends to enforce its rights to this work under copyright laws as a published work Those having access to this work may not copy use or disclose the information in this work unless expressly authorized by Mindray to do so All information contained in this publication is believed to be correct Mindray shall not be liable for errors contained herein nor for incidental or consequential damages in connection with the furnishing performance or use of this material This publication may refer to information and protected by copyrights or patents and does not convey any license under the patent rights of Mindray nor the rights of others Mindray does not assume any liability arising out of any infringements of patents or other rights of third parties Content of this manual is subject to changes without prior notice PROPERTY
43. BW Press E N1 21 to select tem to run Figure 5 58 2 Press ENTER to empty baths Mantenance war once 00 53 Otuent Pime E Z Cleanser Cleaning Rinse Prime Lyse Test Lyse Pome Clean Baths Zap Apertures ui Empty Batns Flush Apertures Empty Tubing Probe Cleanser Clearwng Wipe Block Cleaning Emptying WSC and RBC baths m me Figure 5 59 3 Remove the three fixing screws of the top plate and then remove the top plate Auto Hematology Analyzer Service Manual V1 1 5 27 System Structure fixing screws Figure 5 60 4 Disconnect the connecting tubing of V11 or V12 Record the connecting positions of tubing disconnect the connector wire of V11 or V12 a Valveii Valvel2 Figure 5 61 5 Remove the two fixing screws of the valve to take out V11 or V12 5 28 Auto Hematology Analyzer Service Manual V1 1 Figure 5 63 6 Replace the old valve with new one Connect the connector of driving wire of valve Connect the tubing to the valve Install the valve to instrument 7 After that press ENTER to prime the diluent into baths Auto Hematology Analyzer Service Manual V1 1 System Structure 5 29 System Structure 9 Test V11 or V12 by selecting Service Valve Test and check if the valve can work normally 5 30 Martensece Wat WHORE E Dikent Prime E Z Cleanser Cleaning Rinse Pome Lyse Test Lyse Prime Go Ciean Baths Zap Apertures Errgty
44. Hematology Analyzer Service Manual V1 1 5 23 System Structure When replacing the WBC bath you must replace the tubing connected to the metal junction Figures 5 50 Remove HGB assembly Figures 5 51 5 24 Auto Hematology Analyzer Service Manual V1 1 System Structure Figures 5 52 Remove the WBC aperture Remove the two screws and then take out the aperture between two washers Figures 5 53 Figures 5 54 A noteA The concave side of the aperture must face to the front bath Auto Hematology Analyzer Service Manual V1 1 5 25 System Structure 5 1 7 Clean or Replace V11 or V12 Valve ZN WARNINGA Potential biohazard Open the front panel 1 Push the switch and open the right side door of the machine Figure 5 55 2 Lift up the switch and open the front panel Figure 5 56 3 Figure 5 61 shows the machine with the front panel opened Figure 5 57 5 26 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 1 8 Replace V11 or V12 1 In Menu operation move the cursor to the Service Maintenance item press ENTER to access the Maintenance screen Press lt 1 gt to move the cursor to Empty Baths RITI Mairtensece war ca 00 53 Osuert Prime E Z Cleanser Cleaning Rinse Pnme Lyse Test Lyse Prime Clean Baths Zap Apertures GF Errety Baths Flush Apertures Errgty Tuteng Probe Cleanser Cleaning Wipe Block Clearing Empty WBC and RBC baths
45. O Valve Drive 4 Q_VAL1 Valve Drive 6 Q_VAL2 Valve Drive 8 Q_VAL3 Valve Drive 10 Q_VAL4 Valve Drive 12 Q_VAL5 Valve Drive 14 Q_VAL6 Valve Drive 16 Q_VAL7 Valve Drive 18 Q_VAL8 Valve Drive 20 O VAL9 Valve Drive 22 O VAL10 Valve Drive 24 O VAL11 Valve Drive 26 O VAL12 Valve Drive 28 O VAL13 Valve Drive 30 O VAL14 Valve Drive 32 O VAL15 Valve Drive 34 O VAL16 Valve Drive 36 O VAL17 Valve Drive 38 O VAL18 Valve Drive 40 O VAL19 Valve Drive 42 O VAL20 Valve Drive 44 O VAL21 Valve Drive 46 O VAL22 Valve Drive 48 O VAL23 Valve Drive 50 PGND GND 1 3 49 12VP 12V J2 Pump Drive PIN Defined Explanation 1 12VP 12V 2 O PUMPO Pump Drive 3 12VP 12V 4 O PUMP1 Pump Drive 5 12VP 12V 6 Q_PUMP2 Pump Drive 7 12VP 12V 8 Q_PUMP3 Pump Drive 5 48 Auto Hematology Analyzer Service Manual V1 1 J3 Pumps amp Valves Control System Structure PIN Defined Explanation 1 VAL17 Valve Control 2 VALO Valve Control 3 VAL18 Valve Control 4 VAL1 Valve Control 5 VAL19 Valve Control 6 VAL2 Valve Control 7 VAL20 Valve Control 8 VAL3 Valve Control 9 VAL21 Valve Control 10 VAL4 Valve Control 11 VAL22 Valve Control 12 VAL5 Valve Control 13 VAL23 Valve Control 14 VAL6 Valve Control 15 DVCC Digital GND 16 VAL7 Valve Control 17 DVCC Digital GND 18 VAL8 Valve Control 19 DVCC Digi
46. OF SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD ALL RIGHTS RESERVED Responsibility on the manufacturer party Mindray is responsible for safety reliability and performance of this equipment only in the condition that Auto Hematology Analyzer Service Manual V1 1 i e all installation expansion change modification and repair of this equipment are conducted by Mindray qualified personnel e applied electrical appliance is in compliance with relevant National Standards e the instrument is operated under strict observance of this manual A Note A This equipment is not intended for family usage This equipment must be operated by skilled trained clinical personnel A Warning A It is important for the hospital or organization that employs this equipment to carry out a reasonable maintenance schedule Neglect of this may result in machine breakdown or injury of human health Upon request Mindray may provide with compensation necessary circuit diagrams calibration illustration list and other information to help qualified technician to maintain and repair some parts which Mindray may define as user serviceable ii Auto Hematology Analyzer Service Manual V1 1 Warranty Workmanship amp Materials Mindray guarantees new equipment other than accessories to be free from defects in workmanship and materials for a period of one year from date of shipment under normal use and service Mindray s obligation under this
47. P3 1 Power input range 10 18 V 2 GND JP3 2 Power and signal ground Power supply for heating thermal head and drive motor 7 8 V 8 4 V 4 VCC U1 14 5 V supply 4 75 5 25 V CPU reset signal At high level gt 2 4 V 5 RESET U3 10 after power on 3 VPP U7 8 Auto Hematology Analyzer Service Manual V1 1 3 15 Hardware 3 6 Volumetric Metering Board Functions and Modules The volumetric metering board can calculate liquid volume with the help of volumetric tube and optical coupler It is used to ensure the accuracy of WBC and RBC PLT count The volumetric metering board has two channels WBC channel and RBC PLT channel Each channel consists of one volumetric tube and two optical couplers When the system starts counting the liquid starts flowing in the volumetric tube When the liquid is passing through the first optical coupler the comparator outputs signal of starting count After the liquid is passing through the second optical coupler the comparator outputs the signal of stopping count In this way we can know the time when the system starts and stops counting process The circuit has four parts 1 Circuit for driving optical coupler provide driving current for the four optical couplers Besides its activity is controlled by CPU board in order to decide whether the optical coupler works 2 Circuit for detecting signal of optical couplers this circuit collects the signal of voltage variation wh
48. PIN Defined Explanation 1 GND 3 3VGND 2 VDD 3 3V 3 LED LED Indicator Control 4 NC 5 4 6 MTB Connector Defined J1 MTB Board Connector PIN Defined Explanation 1 5V 0 5V 2 WBC_START WBC Start Count 3 DGND DGND 4 WBC_STOP WBC Stop Count 5 NC NC 6 RBC_START RBC Start Count 7 PGND PGND 8 RBC_STOP RBC Stop Count 9 12V 12VP 10 CTL CNT Detect sensor Auto Hematology Analyzer Service Manual V1 1 Adjustment Chapter 6 Adjustment This section introduces how to adjust the gain of the channels 6 1 General Adjust the Gain of the Channel in the Following Situations Replace count bath RBC count bath RBC PLT WBC count bath WBC whole blood WBC prediluted and HGB Replace aperture RBC aperture RBC PLT WBC aperture WBC whole blood WBC prediluted Replace analog signal board WBC whole blood WBC prediluted RBC HGB and PLT Re install system software HGB Replace HGB unit HGB 6 2 Adjusted procedures Access the Gain Screen Access the Password screen and set the password to 3210 Pree Court Time Patert Linets Trremeson dj Core Dan amp Tine Ul Semple More Gyn Aan Cien Tine gt Rasp ibp Oxe id Pre Capon Figure 6 1 Auto Hematology Analyzer Service Manual V1 1 6 1 Adjustment Password READY who VU 15 48 Password even Buoj Press omoes Figure 6 2 Press MENU key to access
49. PSON LX 300 EPSON LX 300 Scanner optional TYSSO CCD 82 Reagents Required DILUENT M 30D DILUENT RINSE M 30R RINSE LYSE M 30L LYSE E Z CLEANSER Enzyme cleanser M 30E CLEANSER PROBE CLEANSER M 30P CLEANSER Power Input AC 220V 10 AC 110V 10 50 60 1 Hz 50 60 1 Hz Consumption 180 VA 180 VA Fuse 2A 4A Ambient Temperature and Humidity Temperature 15 C 35 C 590 950 Humidity 10 85 without condensation Dimensions Height Width Depth 46cm 39cm 40cm Weight 25KG Recommended Anticoagulant A salt of K EDTA with the proper proportion of blood to anticoagulant as specified by the tube manufacturer Sample Identification An 8 digit identification number is mandatory sample identification Auto Hematology Analyzer Service Manual V1 1 1 5 General Results Output The system can transmit sample and control data to an external computer Sample results screen shows sample identification number sample mode sample results and any sample result flags The system provides a printout of all data 1 6 Auto Hematology Analyzer Service Manual V1 1 General 1 4 Panel Description 1 4 1 Front Panel and Keys RJ Ji Figure 1 1 No Name Description 1 Display Screen Display various messages measured data and histograms 2 Keypad Touch key all of the description refer to under lists 23 buttons 3 Recorder Print out measured result 4 Power
50. Select jumper 10 DSO 11 GND Digital Ground 12 PST3 13 PST2 14 PST1 15 PSTO 16 GND Digital Ground 17 JU3 2 18 PSTCLK 19 GND Digital Ground 20 ITA J20 Keyboard Connector 5 46 Auto Hematology Analyzer Service Manual V1 1 PIN Defined Explanation 1 KEYINO 2 KEYIN1 3 KEYIN2 4 KEYIN3 5 KEYIN4 6 KEYIN5 7 KEYIN6 8 BUZZER 9 KOUTO 10 KOUT1 11 KOUT2 12 KOUT3 13 V5P0 5V 14 GND Digital Ground 15 GND Digital Ground 16 PGND Power GND 17 PGND Power GND 18 12VP 12V System Structure 19 12VP 12V 20 LCDBCTL LCD Background brightness control J21 A D Control Signal J22 Start Key PIN Defined Explanation 1 V5P0 5V DDC 2 GND Digital Ground 3 XCHS4 4 DGAIN2 5 XCHS3 6 DGAIN1 7 XCHS2 8 DGAINO 9 XCHS1 10 DBURNO Zap Control 0 11 XCHSO 12 DBURN1 Zap Control 1 13 HGB_LIGHT1 HGB light Control 14 DCONSTO Constant Current control PIN Defined Explanation 1 KEYIN5 KEYINO 2 KEYIN5 3 KEYOUT3 4 KEYOUT3 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 4 2 Power Driver Board Connectors Defined Ji Valves Drive PIN Defined Explanation 2 Q_VAL
51. U41 U35 and U36 are 8 bit A D used respectively to detect PLT and RBC The sampled data are separately saved into U39 and U38 U38 U41 are 8 bit and 2K FIFO When FIFO is half full it sends interrupt to CPU CPU can read the data from FIFO via FPGA U42 Besides U42 is used to control the analog signal board including gain control zap control HGB light and constant current source control Display Module The display circuit is made up of FPGA U43 and VRAM U47 U49 CPU writes the display data into VRAM via FPGA FPGA can generate LCD driving timing CPU reads data from VRAM in terms of the driving timing and displays them on LCD PCB Layout of CPU Board 3 4 Auto Hematology Analyzer Service Manual V1 1 Hardware c ZA se 3 Wire Serial Port mp rive Valve and Pum D Power Supply cr na Recorder RA Kevboard RS 232C RS 232C y Serial Port Serial Port Parallel Port PS 2 1 2 A A IL E Ki te Q 3 85 IAU ACI za a S on a Josuag pinbrT J9BJJAUJ GOT bo E s lt JOPIOIOY pedioy 11215 Pai 0 ey 4 Figure 3 2 3 5 Auto Hematology Analyzer Service Manual V1 1 Hardware 3 2 Analog Signal Board Functions and Modules The main functions of the analog signal board are 1 Process the original s
52. VP Auto Hematology Analyzer Service Manual V1 1 System Structure 5 4 Connect Circuit Boards RI Ny NU Figure 5 87 Auto Hematology Analyzer Service Manual V1 1 5 41 System Structure 39 36 37 s Figure 5 88 No Socket Plug No Socket Plug 1 J1 07094 21 Shield Wire 2 J2 07075 22 J5 07100 J13 07095 23 J6 07102 From Pressure 4 J4 07076 24 U34 Chamber From Vacuum 5 J6 07078 25 U26 Chamber 6 J7 10ml 26 J18 3001 21 07098X 7 J8 50ul 27 J22 07085 8 J12 28 J6 07091 9 JP1 Jumper 29 J16 07087 10 J10 07087 30 J4 3001 21 07093X 11 J8 5V 31 J9 07092 12 J20 3001 21 07116C 32 J11 07092 13 J21 07115 33 J3 3001 21 07093X 14 J3 3001 21 07108X 34 J9 07091 15 J8 5V 35 J15 19459A 16 J7 From Transformer 36 J14 C 3001 21 07105 17 J2 3001 21 07114X 37 J1 C 3001 20 18452 RBC DiskOnModule Disk 18 3001 21 07103X 38 J14 Socket 19459A WBC 19 3001 21 07103X Socket 20 J1 3001 21 07114X 5 42 Auto Hematology Analyzer Service Manual V1 1 5 4 1 CPU board connectors defined J2 No use J3 FDD connector J4 Valves and pumps signal connector Figure 5 89 PIN defined Explanation 1 ALVE17 Value s drive signal 2 IVALVEO Value s
53. a new chamber W WBC always clog 1 WBC bath is dirty 1 Clean the bath 2 The count time is a little longer than the setting time 3 One of the sensor which on the MTB board is broken using E Z cleanser or probe cleanser 2 Re set the count time 3 Replace the MTB board WBC bath leakage WBC bath is broken with Replace the WBC bath cranny WBC background and value is WBC bath is broken with Replace the WBC bath too high cranny WBC bath full and liquid The V12 valve doesn t work Clean or replace the overflows or dirty or doesn t work well V12 valve WBC result is not stable WBC bath is broken with Replace the WBC bath cranny Wipe block is leakage Wipe block is old Replace wipe block WBC value is too high and Mis use the Rinse and Exchange the two RBC is zero or diluent reagents WBC clog no count time WBC aperture clog Clean the aperture using probe cleanser WBC differential part is abnormal and its end point at 200fl WBC channel gain is not correct Adjust WBC channel gain WBC bubbles during counting SV8 valve is leakage Replace the SV8 valve WBC no result Analog board is broken Replace the analog board 2 26 Auto Hematology Analyzer Service Manual V1 1 2 6 Alarm Troubleshooting Error Message Possible Cause Com Error The transmission settings between BC 3000PLUS and the external co
54. agent and tighten the cap of the reagent bottle The constant current is not Change the transformer or analog board stable The diuent syringe exists Remove the bubbles from the syringe bubbles Faulty the Analog board Replace the analog board Poor Reproducibility for RBC Possible Cause Countermeasure The background data on RBC is high Refer to Background Noise Check of this chapter Dirty RBC measurement bath Clean the RBC bath with E Z cleanser or probe cleanser Dirty measuring tube Clean the tube below the RBC bath Dirty wipe block Clean the wipe block Replace a new wipe block The sample probe position is not correct Adjust the probe position using localizer The 3 way valve SV11 cannot drain liquid empty Replace the 3 way valve SV11 2 20 Auto Hematology Analyzer Service Manual V1 1 Troubleshooting The 3 way valve SV11 is dirty Clean the valves Replace this valve The constant current is not stable Change the transformer or analog board The diuent syringe exists bubbles Remove the bubbles from the syringe Faulty the Analog board Replace the analog board Auto Hematology Analyzer Service Manual V1 1 Troubleshooting 2 5 Troubleshooting B Burn fuse when power on unit AC input power is not stable Using a manostat Power supply boar
55. al couplers on the volumetric metering board are damaged or the values of potentiometers are improperly set The liquid cannot flow in the tubing smoothly pressed bends or clogged by foreign object Inadequate reagent Can not form stable surface in RBC metering glass tube The tubing has leaks or the vacuum system has problem The sample has problem such as the type and proportion of anticoagulant is selected improperly or there is blood clots RBC Bubbles RBC reference count time is set up improperly Optical couplers on the volumetric metering board are damaged or the values of potentiometers are improperly set Inadequate reagent Can not form stable surface in RBC metering glass tube The tubing has leaks or the vacuum system has problem Diluent Empty There are no diluent in the diluent container The liquid sensor is not connected correctly The liquid sensor is damaged Rinse Empty Lyse Empty Pressure1 Low 0 There is no rinse in the rinse container The liguid sensor is not connected correcily The liguid sensor is damaged There are no lyse in the lyse container The liguid sensor is not connected correcily The liguid sensor is damaged Pressure pump has fault Tubing vacuum chamber has leaks Auto Hematology Analyzer Service Manual V1 1 2 29 Troubleshooting 11 12 Tubing connecting vacuum chamber to sensor loosens or falls off The valve connec
56. are connected through to identify the key function 2 Circuit for controlling LCD backlight this circuit first uses a piece of 7805 to obtain the constant 5V supply Then it can acquire the voltage used to control the backlight brightness through using 2 resistors with fixed resistance and an adjustable resistor The voltage division range is between 1V 3V LCDBCTL signal is used to control the existence of backlight 3 Buzzer use control signal I7 and a dynatron to control the buzzer Block Diagram of Keypad IA I6 Key Matrix 00 03 Circuit for controlling Basverc nuit LCD backlight Figure 3 5 Auto Hematology Analyzer Service Manual V1 1 3 13 Hardware 3 5 Recorder Board Functions and Modules The main function of the recorder board is to receive the data from the CPU board via serial port process them and send them to the thermal head At the same time it drives the motor of the thermal head to feed paper so that the received data can be printed out in the form of characters or graphs Block Diagram of Recorder Board Thermal Head Motor CPLD Status Driver 9536 Detection DC DC 12V gt 8V CPU Signal amp 5V Figure 3 6 Thermal head The thermal head the core component in the recorder is the PTMBL1306A thermal head manufactured by the ALPS company CPU system
57. cimal point Press ENTER to exit edit status and access the Count status 2 12 Auto Hematology Analyzer Service Manual V1 1 Troubleshooting Whole Blood Cakbrason day ero 05 22 1 Edt anoj TO Fol to enter XI to select tem X to move cursor in tem ENTER ext ect Figure 2 5 Run Calibrator Procedure ZN WaRNINGA Potential biohazard ZN WaARNINGA Avoid contacting with the sample probe 1 Place the well mixed calibrator to the probe so that the tip is well into the tube and press the START to run The process of run is the same as that of count After running the screen displays as shown in below figure FF lt a Whole Sood Calibration Eso wate 09 40 m Corte resuts 1 7 646 Yes Exp Date 2003 a ias a ow ras 10 2 0 1 4 12 12 129 1 Ede gems Figure 2 6 Select Yes to validate the results Auto Hematology Analyzer Service Manual V1 1 2 13 Troubleshooting Select Cancel to invalidate the results The below figure shows an example of the results after five times of running Whole Blood Cal raton READY wna UTO9 46 1 Ede Suen Auto Calibration Finished Figure 2 7 The average value of calibration and the new calibration factors automatically calculated by the system are displayed in the right side of the screen refers to invalid results which means that the average value of the parameter and the calibration fac
58. cursor in tem Figure 6 11 After entering the new calibrating factor press MENU key to return to menu operation The dialog box as shown below will pop up TUT Factory Whole Blood Galibeation nab wnour 17 40 102 2 2003 04 26 105 7 20030426 105 1 20030426 100 0 20030426 1086 20080426 KSEE ove povp AA Figure 6 12 Select Yes the system will save the new calibrating factors 2 Set the user calibrating factor of MCV to 100 Access the Password screen and set the password to 0000 Auto Hematology Analyzer Service Manual V1 1 6 7 Adjustment Figure 6 13 Password 0000 Baw Pessi I lomoecnen o o SS S Figure 6 14 Access the Calibration Manual Calibration screen set the calibrating factor of MCV to 100 Count Wy Sample Mode Review Quality Control KF Setup A Service Calibration MINDRAY CO LTD v v v T a Shutdown imenu Figure 6 15 O Whole Blood Castration mor w 0A Factor Time 102 3 2003 04 26 100 0 2003 04 26 100 0 0030426 100 0 20080426 100 0 20030426 uno Press 111 1 to select tem X to move cursor in tem Figure 6 16 Auto Hematology Analyzer Service Manual V1 1 Adjustment After entering the new calibrating factor press MENU key to return to menu operation The dialog box as shown below will pop up Whale Blood Calibration rawr oro TOs 497 wec 100 10
59. d Board Indicator Board Sample Probe Assembly Syringe Assembly CAP Component For Lyse CAP Component For Diluent CAP Component For Rinse RBC Bath WBC Bath Sample Probe Wipe Block Mini Switch Assembly Vacuum Chamber Power Supply Board 220V Power Supply Board 110V Recorder Board TR60 D Recorder Board Analog Signal Board Pressure Pump Auto Hematology Analyzer Service Manual V1 1 8 1 Spare Part list 8 2 M90 100032 3001 30 07175 900E 10 04913 2000 10 03061 3001 10 07149 59BR 10 08830 M30 000015 A30 000001 3001 10 07068 M90 100035 M90 100071 0000 10 10828 3001 20 07247 A22 000005 2 5ml Syringe HGB Unit Inverter CXA L0612 VJL TDK Inverter CXA L0612 VMR TDK Thermal head THERNAL EPL2001S2 Thermal head ALPS PTMBL1306A Thermal Print Paper 58mm Width Thermal Print Paper 50mm Width Tubing lD1 16 OD1 8 Tubing ID0 02 ODO 06 Tubing ID3 32 OD5 32 DiskOnModule Disk 64M Localizer Sample Cup Auto Hematology Analyzer Service Manual V1 1 Performance Test Chapter 9 Performance Test After disassembling or replacing parts or troubleshooting the following testing must be carried out in order to ensure that the instrument can operate correctly Do not use any results reported by HEMATOLOGY ANALYZER for medical and clinical diagnosis before all performance items are satisfied requirement Background Check The background result must satisfy the specificatio
60. d is short voltage regulator circuit Replace the power supply board Bubbles Bubbles in the sample Remove the bubbles Regents are not enough Re prime the regents Setting count time is long Re set the count time The aperture is broken Change a new aperture Counting channel is leakage Replace the leakage parts Background testing is Regents are dirty Change new regents abnormal Electronic noise Connect ground well Dirty bath Clean the bath Dirty valves Clean the valves C Counting time is sometimes The CPU board has some Replace the CPU too short and sometimes problem board normal Clog Big cells or debris in the Remove the debris sample from the sample Setting count time is short Re set the count time The aperture is blocked Clean the aperture Diluent is not enough Check the diluent syringe and diluent Volumetric board is broken Replace the volumetric board D Diluent injection time becomes longer and make WBC bath full SV3 valve doesn t close properly Replace the SV3 valve Display is not clear Backlight is too bright or too dark LCD screen is old Adjust the unique resistor on the CPU board Replace screen the LCD F Fluctuating and rotatory motor error The environment temperature is low Tubing which on the sample Increase environment TEMP and add UPS Loose the tubing Auto Hematology Analyzer Service Manual V1 1 Troubleshooti
61. drive signal 3 IVALVE18 Value s drive signal 4 NALVE1 Value s drive signal 5 IVALVE19 Value s drive signal 6 NBLVE2 Value s drive signal 7 IVALVE20 Value s drive signal 8 NBLVE3 Value s drive signal 9 NALVE21 Value s drive signal 10 NBLVE4 Value s drive signal 11 NALVE22 Value s drive signal 12 IVALVE5 Value s drive signal 13 NALVE23 Value s drive signal 14 VALVE 6 Value s drive signal 15 DVCC power 16 IVALVE 7 value 7 control 17 DVCC power 18 NALVE8 value 8 control 19 DVCC power 20 IVALVE9 Value s drive signal 21 DVCC power 22 IVALVE10 Value s drive signal 23 PUMPO Pump 0 control Auto Hematology Analyzer Service Manual V1 1 System Structure 24 IVALVE11 Value s drive signal 25 PUMP1 Pump 1 control 26 NALVE12 Value s drive signal 27 PUMP2 Pump 2 control 28 NALVE13 Value s drive signal 29 PUMP3 Pump 3 control 30 NALVE14 Value s drive signal 31 DGND Signal GND 32 NBLVE15 Value s drive signal 33 DGND Signal GND 34 IVALVE16 Value s drive signal J5 Serial Port PIN defined Explanation 1 RXD3 Receive DGND Digital GND 3 TXD3 Transmit J6 Serial Port PIN defined Explanation 1 SIN Receive DGND Digital GND 3 SOUT Transmit J8 5V Power Supply PIN defined Explanation 1 GND Digital GND 2 GND Digital GND 3 V5P0 5V 4 V5P0 5V J9 12V 8
62. e baths and cannot be removed easily 5 Electronic noise affecting the counting calculation circuit AC line backlight converter peripheral equipment such as microwave treatment machine motors in the instrument Mechanical noise affecting the counting calculation circuit Vibration from motor in or around the instrument such as a centrifuge 7 Tubing noise affecting the counting calculation circuit Liquid flow with some remained electric charges As a countermeasure to the above 7 causes take the following actions Cause 1 or 2 Replace the diluent with diluent from a sealed container Cause 3 or 4 Clean the baths with E Z cleanser Clean the baths with probe cleanser Auto Hematology Analyzer Service Manual V1 1 Troubleshooting Clean valves with distilled water Exchange the other same model valves to try Cause 5 Securely ground the instrument including any optional units such as external printer Cause 6 Keep the instrument away from the vibration source Cause 7 Securely ground the tubing Auto Hematology Analyzer Service Manual V1 1 2 17 Troubleshooting This subsection describes the cases when the reproducibility for the following measuring parameter or calculated parameter is poor PLT HGB WBC RBC Data other than red cell indexes MCV MCH and MCHC RBC and PLT coefficient related parameters HCT and MCV For Reproduc
63. e for the PLT parameter is smaller than the WBC and RBC parameters f the data for the PLT parameter exceeds 10X10 L do the action described below to reduce the background noise Reducing Background Noise To reduce the background noise when the background check value exceeds the acceptable diluent data shown in the previous table perform the following 1 Make sure connecting grounding well 2 Execute the clean Bath program which in the service menu If this does not reduce the background noise perform the following steps 3 Execute the E Z cleanser cleaning and Diuent Prime program 4 Perform the background check to make sure that the background noise is reduced If the data of the background check is still outside the acceptable diluent data values shown in the previous table replace the diluent with diluent from a new sealed container A noTteA When the instrument is used every day and the background noise rarely exceeds the lower limit for the diluent data the instrument is not severely contaminated However this contamination builds up in the instrument and cannot be easily removed if the instrument is not cleaned periodically Auto Hematology Analyzer Service Manual V1 1 2 5 Troubleshooting Reproducibility Check 2 6 This check is used to check reproducibility of the instrument using pintout data value of a diluted sample from the same hematology control When the values are o
64. e volumetric glass tubes 10 Cover the metal shield box of the volumetric unit 6 18 Auto Hematology Analyzer Service Manual V1 1 Adjustment 6 12 Re calibrating Instrument After setting up the gain calibrate the instrument again Refer to the operator s manual of the instrument to obtain the information about calibration Auto Hematology Analyzer Service Manual V1 1 6 19 Maintenance Chapter 7 Maintenance 7 1 Daily maintenance If the instrument works day and night or the sample gt 100 day the user should execute probe cleanser cleaning operation once every day The step is Press MENU button B Service Probe cleanser cleaning If quantity of samples more than 50pcs the user should at least set the Auto clean time less than 8hours It means the user should execute this program once everyday The step is Press MENU button gt Setup B Auto clean time If the user turns off the instrument every day the user should execute probe cleanser cleaning operation once every three days The step is Press MENU button B Service gt Probe cleanser cleaning Use E Z cleanser to execute shutdown program Refer to instruction of shutdown program Dispose the waste liguid Clean the instrument and TFT screen with wet soft cloth Clean the TFT screen only water or distill water are available otherwise will damage the screen Check the rest reagent
65. ed to aspirate and dilute the sample and clean the system Mm Volumetric Unit This part implements the function of measuring the volume we can calculate the concentration of blood cells with the number of pulses and the value of volume This part is mainly made up of glass tubes and optical couplers M Vacuum Unit Vacuum is the impetus to drive the counting process of hematology analyzer It is made up of vacuum chamber valves and vacuum pump M Pressure Unit The pressure of this part is provided by pressure pump It has the function of mix up dilution M Auxiliary Unit This part mainly refers to the connecting tube connectors and valves which are mainly used for connection 4 2 Auto Hematology Analyzer Service Manual V1 1 Hydraulic System 4 3 Whole Blood Count Cycle In whole blood mode press the Start key the system transmits Start Count request to CPU which will feedback the response signal 1 10 11 The 50ul syringe aspirates 13ul of EDTA K2 anticoagulant whole blood into the probe The instrument reads HGB blank The WBC bath rinses and drains The 10ml syringe dispenses diluent into the WBC bath to prefill it The probe moves to the WBC bath and the 10ml and 50ul syringes dispense the sample 13ul and diluent into the WBC bath making a 1 269 dilution The 50ul syringe aspirates 15 6ul of the 1 269 dilution into the probe for the RBC PLT dilution The RBC bath rinses and drains The 1
66. em software if the upgrade process is failed and the instrument can t start up again Auto Hematology Analyzer Service Manual V1 1 A ppeitig Appendix Hardware Diagram of BC 3000PLUS Rotat Fluctuati 10m1 2 5ml amp 50ul Positi VAL 1 23 PUMP 0 2 Motor Motor Syringe Syringe osso Motor Motor aje Ji J2 JA J6 I7 18 J10 a Valve Pump X Y 10mL 2 5m amp 50uL Sensors LCD Display Valve amp Pump COM Power of Recorder Pow er Drive Board 12V Power Ji J3 J9 J14 J13 Liquid Fani e Z 32 Volumetric JA J6 Jo Sensors G M etering Valve amp Pump COM2 Power of Recorder J16 a Jl Board yj JP2 JI IDE J15 Interface JP5 J18 g CPU Board Z 5 oar a JP4 J J20 w L
67. en the liquid is flowing through the optical coupler and then transmit the signal to the subsequent circuit 3 Circuit for generating comparative level generate the comparative threshold level of the comparator 4 Circuit for outputting comparative signal compare the voltage output from the circuit for detecting signal of optical coupler with the comparative threshold and accordingly output start count or stop count signal Block Diagram of Volumetric Metering Board Detect circuit for WBC start count optical coupler Circuit for generating comparative voltage Circuit for outputting comparative signal Circuit Detect circuit for WBC end Circuit for generating Circuit for outputting for count optical coupler comparative voltage comparative signal driving optical Detect circuit for RBC PLT Circuit for generating Circuit for outputting couplers start count optical coupler comparative voltage comparative signal Detect circuit for RBC PLT Circuit for generating Circuit for outputting end count optical coupler comparative voltage comparative signal Figure 3 7 3 16 Auto Hematology Analyzer Service Manual V1 1 Hardware e Circuit for Driving Optical Couplers To obtain the same light intensity of the four optical couplers we need to use uniform constant current sources The source is made up of U3 and resistor with the output range
68. ent Whole Blood Mode 4 11 4 5 2 Inspire Sample and Diluent Prediluted Mode 4 12 4 5 3 WBC Injection 8 Lyse Preparation nn 4 13 4 5 4 RBC 8 Lyse MECON rii ies cece cessive je AE PEN de da EaP ayeee epatit 4 14 4 MAMO sa oe ie A OE eat alkil bie AKA Dee oak ean i eae 4 15 4 5 6 Count Ae e sei see ne Zaza ksm denih veliki laki io pni s s ikes babes 4 16 ASofCIGANING sti Rn mia au nn nd lab fee muho 4 17 4 5 6 FU Sai S en evade aaa ee Nee lee a beda 4 18 4 519 Empty TUDO i iin viii useen textes Silvey Hea ta ie a a a lial EE iis 4 19 Chapter 5 System SUUCIUIC csccccscwesebadixcecdsneastisyeenecseeyiguehenetenspusurseederecagengexbenss 5 1 5 1 Disassemble Replace Parts and Components ceceeeeseeeeeeeeeeeeeeeneeeeeeeeneeeeeeeas 5 1 5 1 1 Disassemble Syringe and Replace Piston e 5 1 5 1 2 Replace Sample Probe rrii iania nena 5 7 5 1 3 Replace Sample Probe Wipe Block ee t es 5 12 5 1 4 Replace Vacuum Chamber 5 18 5 185 Replace P MP errea eesti aes bei ee ai kA Lae ete ede 5 19 5 Te Replace Count Bath sea ae aa A Se ee et 5 22 5 1 7 Clean or Replace V11 or V12 Valve 5 26 5 1 8 Replace VIT Or V2 cece asec anit ies dic esnek Aves OE veze kej z dedek Pa odk l ro epika 5 27 5129 Glean VAT Or V1 2 sis ni aii eaves ol eat fia shave e Ni obese 5 31 5 1
69. er Service Manual V1 1 1 4 2 Rear Panel 11 10 General 95 8 7 Figure 1 2 No Name Description 1 Keyboard Interface Connect the standard keyboard 2 RS 232C Serial Port 1 Connect computer and transfer data to computer A cauTionA In order to avoid any safety hazard only coonect personal computer which are approved to IEC950 The instrument should only be connected to an external instrument which complies with the CISPR 11 Second Edition 1990 09 Group 1 and Class B standard RS 232C Serial Port 2 Connect a bar code scanner Printer Interface Connect the external printer LX 300 LX 300 DILUENT Tubing Connector Inlet for diluent Connect one end of the tube standard accessory to the diluent inlet and attach the other end of the tube to the diluent 6 BNC socket for DILUENT connector for diluent Connect one end of the connector of the sensor cable 7 BNC socket for RINSE connector for rinse Connect one end of the connector of the sensor cable Auto Hematology Analyzer Service Manual V1 1 1 9 General WASTE Tubing Connector Inlet for waste Connect one end of the tube standard accessory to the waste inlet and attach the other end of the tube to the waste RINSE Tubing Connector Inlet for rinse Connect one end of the tube standard accessory to the rinse inlet and attach the other end of the tube to the rinse 10 Equipotential ground t
70. erminal Connects the ground lead to the Equipotential ground terminal on the wall for earth grounding 11 Power switch AC source Fuse holder Turns power on or off Connects the AC power cord to supply the AC power to the instrument Contains the time lag fuse T 2A for 220V or T 4A for 110v ZA cayTION N Fuses cut the power off when an abnormality occurs in the hematology analyzer Remove the malfunction before replacing the fuse Before replacing a fuse turn the power off anf disconnect the AC power cord from the instrument Fuse replacement should be done by a gualified person Auto Hematology Analyzer Service Manual V1 1 General 1 4 3 Front review without front panel Figure 1 3 1 Fluctuating Motor 2 Sample Probe 3 Sample Probe Wipe Block 4 WBC unit shield 5 RBC PLT unit shield 6 Start key 7 Valve 11 8 Valve 12 9 Floppy Disk Driver Auto Hematology Analyzer Service Manual V1 1 1 11 General 1 4 4 Right side view without the door 23 Figure 1 4 1 valve 8 3 vacuum chamber 5 valve 16 7 valve 13 9 valve 2 11 2 5ml and 50ul motor 13 2 5ml syringe 15 10ml syringe 17 valve 3 19 valve 6 21 valve 17 23 valve 18 22 21 20 19 14 11 18 17 16 15 13 12 2 volumetric unit 4 valve 15 6 valve 14 8 valve 10 10 valve 9 12 10ml motor 14 50ul syringe 16 valve 4 18 valve 1 2
71. ess to modify the auto clean time 6 16 Auto Hematology Analyzer Service Manual V1 1 6 11 Adjust Volumetric Metering Board TEST TEST3 Glass tube TEST 5 TEST 6 TEST 7 TEST 2 Optical coupler Figure 6 18 The volumetric metering board and two glass tubes construct the volumetric unit The Adjustment D1 Indicating light p3 D1 D2 D3 D4 TEST 4 DGND VRI Potentiometer VRI VR2 VR3 VR4 YRS volumetric metering board consists of four optical couplers five potentiometers and four indicating lights The four indicating lights correspond respectively to the four optical couplers When there is no liguid inside the glass tubes the lights are off When the liguid surface passes through the optical coupler the corresponding light will light on Optical Coupler Indicating Light Potentiometer Test Point WBC Start D1 VR1 TEST1 WBC Stop D2 VR2 TEST2 RBC Start D3 VR3 TEST 3 RBC Stop D4 VR4 TEST4 The volumetric metering board should be adjusted in the following status 1 The volumetric metering board has been changed 2 The parts of the volumetric metering board have been changed 3 The optical couplers status can t correspond to the liguid surface in the glass tubes because the parts are aging Tools Auto Hematology Analyzer Service Manual V1 1 6 17 Adjustment 1 2 digital multimeter slotting screwdriver The procedures of adjusting t
72. f most blood samples between 320 350fl through adjusting the digital potentiometer of WBC whole blood channel See figure 6 5 W wW B B c Lely O 50 100 150 200 250 300 fL 6 50 100 150 200 250 300 fl W B c o 50 100 150 200 250 300 ra O 50 100 150 200 250 300 fL W W B B c B LCI ZER H 0 50 100 150 200 250 300 fL O 50 100 150 200 250 300 fL Figure 6 5 W B c A i i 0 50 100 150 200 250 300 fL O 50 100 150 200 250 300 fL Figure 6 6 Ww Ww B B Cilj ce i 0 50 100 150 200 250 300 fL O 50 100 150 200 250 300 fL Figure 6 7 See figure 6 4 press 1 to move the cursor onto WBC whole blood option press to change its value The greater the current value is the larger the channel gain will be Analyze more than 5 fresh EDTA Kz anticoagulant venous blood specimens of healthy persons in whole blood mode Controls or calibrators cannot replace the blood specimens 6 4 Auto Hematology Analyzer Service Manual V1 1 Adjustment After confirming the gain of WBC whole blood channel set up the value of WBC prediluted channel so as to make it the same as the value of WBC whole blood channel Analyze more than 5 fresh capillary blood specimens of healthy persons in preduiluted mode If most of the WBC histograms are satisfied the gain of WBC prediluted
73. ff the system otherwise the whole system program may be destroyed and the system will not start up again The procedures are as the following 1 Turn on the hematology analyzer and wait until the system accesses the Count screen In menu operation move the cursor onto Setup Password and press Enter key to access Password screen Enter 3210 and then return to Count screen Insert the floppy disk with HEMATOLOGY ANALYZER upgrade software into the floppy disk driver Press Shift F8 pressing Shift key and F8 key on the keyboard at the same time the HEMATOLOGY ANALYZER system software will read the floppy disk and upgrade the program automatically After the upgrade process has finished the system will start up automatically If the upgrade software on the floppy disk cannot be read or found the system will report error and return Count screen automatically In the process of upgrading the files if Floppy open error is reported please check if the floppy disk has been inserted and if the floppy disk driver connection is correct If Read floppy disk error or No update files is reported please replace the floppy disk with correct data and execute the operation again If the upgrade process is failed the system will automatically restore the software with original version report error and return Count screen automatically The DiskOnModule disk must be replaced by a new one with the syst
74. gn TU 19 serol 30 pogo ENTER 10 et Figure 10 17 10 2 2 Abnormal Pulse Graphs Severe interference in WBC channel Data length increases obviously background whe length 1577312 current potibon 1434976 Prose E to show nder r Jir to scroll page TJU to seret 30 pages ENTER to est Figure 10 18 Severe interference in WBC channel 10 6 Auto Hematology Analyzer Service Manual V1 1 Histograms and Pulse Graphs Data length increases obviously normal sample whe length 7205314 current postion 35840 i f a Press to show ruber fhi so scrot page TICL so scrot 30 pages ENTER to Figure 10 19 Severe interference in RBC channel Data length increases obviously background current pore 13824 o JJ vere Press K to show ruler m jin to serol page T 1 to serol 30 pages ENTER to exit Figure 10 20 Severe interference in RBC channel Data length increases obviously normal sample rbe length 1123968 Current posmom 7680 IM WO Press 3 to show ruler f Y to sil i page XI to smol 10 pages ENTER to ezt Figure 10 21 Severe interference in PLT channel Data length increases obviously background Auto Hematology Analyzer Service Manual V1 1 10 7 Histograms and Pulse Graphs pit length 2048000 Current pornon 120 E TEE IN Prese Ej to show rate feje to scroll 1 page T1 to scr
75. gy Analyzer Service Manual V1 1 10 9 Histograms and Pulse Graphs abe length 41953 careat pornon 233984 fji to scrot 1 page T 1 to sarot 30 pages ENTER to ent Figure 10 28 Sample of too dense concentration in RBC channel Does not occur in normal situation rhe length 1024000 current portion 8192 Monk m m vesna oo Eto show nare e to sero poga TBL to Perot 39 pages ENTER to ext Figure 10 29 e Slight interference in PLT channel Data length does not increase obviously normal sample current postion 179200 Press ff to show rder frji to scroll 1 page TN to serol 30 pages ENTER to ext Figure 10 30 Sample of too dense concentration in PLT channel Does not occur in normal situation 10 10 Auto Hematology Analyzer Service Manual V1 1 Histograms and Pulse Graphs Figure 10 31 Interference in WBC channel caused by inverter Feature sine wave with cycle of 20 26us Current poston 564224 Proda T to chow nder fe Jie to serol 1 page 1 4 to scroll 30 pages ENTER to ext Figure 10 32 Measuring interference from inverter whe length 382976 Cutest poston 370688 pterral an tagh 100 125 to select ruter to bade rules i to move epoce TILL to move 10 specon ENTER to ext Figure 10 33 e Insufficient liquid in WBC bath during count Auto Hematology Analyzer Service Manual V1 1 10 1
76. he volumetric metering board 1 Remaining the instrument running 2 Remove the metal shield box of the volumetric unit 3 Use the DC 20V level of the digital multimeter connect the red pen toTEST6 and the black pen to TEST7 Adjust potentiometer of VR5 to make the voltage is 0 55 0 02V 4 Execute the Rinse Prime in Service Maintenance screen to fill the liquid into the glass tubes Use the DC 20V level of the digital multimeter connect the black pen to DGND and use the red pen to measure TEST1 TEST2 TEST3 and TEST4 The voltage of each point should be higher than 3 0V 5 If any voltage is lower than 3 0V adjust the corresponding potentiometer of VR1 VR2 VR3 or VR4 to make the voltage higher than 3 0V 6 Press the support rods of valve V7 and valve V17 to empty the liquid in the glass tubes Use the DC 20V level of the digital multimeter connect the black pen to DGND and use the red pen to measure TEST1 TEST2 TEST3 and TEST4 The voltage of each point should be lower than 2 0V 7 If any voltage is higher than 2 0V adjust the corresponding potentiometer of VR1 VR2 VR3 or VR4 to make the voltage lower than 2 0V 8 Repeat procedures 4 through 7 until that if there is liquid in the glass tubes the four indicating lights are on and if there is no liquid in the glass tubes the four indicating lights are off 9 Run background count affirm that the count start time and stop time correspond to the liquid surface in th
77. ibility 2 18 Auto Hematology Analyzer Service Manual V1 1 Troubleshooting Poor Reproducibility for PLT Possible Cause Countermeasure The background data on PLT is high Refer to Background Noise Check of this chapter Dirty RBC aperture Clean the aperture Dirty RBC bath Clean the bath Refer to the Maintenance of the operator s manual Dirty measuring tube Clean the tube below the RBC bath Dirty wipe block Clean the wipe block Replace a new wipe block The sample probe position is not correct Adjust the probe position using localizer The 3 way valve SV11 is dirty Clean the valves Replace this valve The 3 way valve SV11 cannot drain liquid empty Replace the 3 way valve SV11 The diuent syringe exists bubbles Remove the bubbles from the syringe Faulty circuit Replace the ANALOG board Poor Reproducibility for HGB Possible Cause Countermeasure Dirty WBC measurement bath Clean the WBC bath with E Z cleanser or probe cleanser The voltage output from the HGB sensor is not optimal Adjust the HGB voltage Refer to Adjust Gain of this manual Adjust the adjustable resistance VR3 8 VR4 to adjust the output voltage of HGB to 4 4v 4 6v The 3 way valve SV12 cannot drain liguid empty Replace the 3 way valve SV12 The 3 way valve SV12 is dirty Clean or replace the valves
78. ifier to amplify the signal The circuits for measuring pressure and temperature 3 8 Auto Hematology Analyzer Service Manual V1 1 Hardware are basically the same The following description uses pressure measuring circuit as the example The circuit uses U25 to construct the constant current source to power the pressure sensor The pressure sensor has bridge circuit Its output signal is transmitted into U20 by means of difference U20 can adjust the AMP multiple via the adjustable resistor at its Rg pin so as to amplify and output the signal Additionally an adjustable resistor is connected to the output of the pressure measuring circuit to adjust the zero output of the pressure sensor Power Supply Monitor The 12V voltage powering the analog circuit is converted from 5V voltage This part is realized by using DC DC module U30 Add a LC filter circuit at the end output of the power supply to reduce its ripple On this board relay is used to control ON OFF of the 120V AC so as to zap the apertures 48V voltage is converted from the 53V AC voltage This part first uses DB104 to implement DC conversion then use U29 to obtain the 48V DC voltage Optical coupler is used to control ON OFF of the supply The monitor circuit adopts the form of voltage division with resistor Its output is transmitted to the CPU board by a follower The power supplies to be monitored are 48V and 12V Auto Hematology Analyzer Service Manua
79. ignal into the situation matching the requirement of A D conversion 2 Amplify the weak signal of WBC and RBC PLT channels to the value between 0 2V 5V 3 Amplify HGB signal 4 Monitor the environmental temperature 5 Monitor the pressure and vacuum in chambers 6 Monitor the accessorial power supplies The analog signal board includes the following modules 1 RBC PLT and WBC amplification circuit blood cells count channel including WBC unit and RBC PLT unit This circuit can amplify the WBC and RBC PLT signals by using multistage AMP and band pass filters Every channel in this circuit uses digital potentiometer to adjust the AMP multiple There is voltage limited protective output at the end stage of output circuit 2 HGB measuring circuit amplify HGB signal via using current voltage conversion and voltage AMP circuit At the same time it acts as the constant current driver circuit for the LED when measuring HGB The output current of this circuit is adjustable between 5 25mA ON OFF of the constant current source is controlled by optical coupler This circuit uses digital potentiometer to adjust the AMP multiple 3 Pressure measuring circuit This circuit can transfer the changes of vacuum and pressure into voltage signal by pressure sensor then amplify this signal and send it to A D converter on CPU board to acquire digital signal The AMP circuit uses AD620 The adjustable resistors are placed at the output and the feedback
80. in order to effectively use the instrument in order to effectively use the information in the service manual Auto Hematology Analyzer Service Manual V1 1 1 1 General 1 2 Service Policy A cauTIONA Be careful not to directly touch any place where blood is or may spread to Wear rubber gloves to protect yourself from infection before doing maintenance Our company s basic policy for technical service is to replace faulty units printed circuit boards or parts We do not support component level repair of boards and units outside the factory A noTeA When ordering parts or accessories from your nearest distributor please quote the part number and part name which is listed in the service manual and the name or model of the unit in which the required part is located This will help us to promptly attend to your needs Always use parts and accessories recommended or supplied by our company to assure maximum performance from your instrument 1 2 Auto Hematology Analyzer Service Manual V1 1 1 3 Specification Hemoglobin Analysis Wavelength 525nm Sampling Features Volumes Required for Each Analysis Whole Blood Mode vein blood Prediluted Mode capillary blood Aspirated volumes 500uL of lyse first dilution per cycle for WBC measurement 13uL 20uL 300uL of second dilution per cycle for RBC and PLT measurement Dilution Ratios Whole Blood Prediluted WBC HGB 1 308 1 407 RBC PLT 1 4483
81. ine on the screen CPU board is broken if the line is a dot line and the position is fixed LCD screen is faulty if the line is a continuous black or light line Replace the CPU board Replace the LCD screen Auto Hematology Analyzer Service Manual V1 1 Troubleshooting LCD screen is dark The color palette setting is not correct The backlight is too dark Re set the color palette to 8 color Adjust the unique resistor on the CPU board sometime normal Leakage Vacuum chamber is broken Change the vacuum chamber waste liquid tubing is kink Loose the waste liquid tubing tubing leakage Check the default point Analog board is broken Replace a new analog board The air filters are dirty Replace this two fiters M mid size cells percent is Putthe sample to longtime Count the sample in sometime too high and Add too much anti coagulant 30mins Reduce the value of anti coagulant N No diluent injected Diluent is empty The piston falls off Change a new bottle of diluent Re fix the piston No sample inspired and no diluent injected SV4 valve doesn t work Replace the SV4 valve sensor is broken or plastic washer falls off No initialization but exist The CPU board is broken Replace the CPU backlight and a moment it board becomes black the indicators flash like saver No rinse diluent lyse alarm The rinse diluent lyse Rep
82. ive Signal J9 Serial Port PIN Defined Explanation 1 TXD PC Transmit 2 DGND Digital GND 3 RXD PC Receive J10 Position Sensors Connector PIN Defined Explanation 1 P1 803 Rotatory motor s position 1 2 P2 803 Rotatory motor s position 2 30 4 GND GND 5 SD1 Rotatory motor s position 1 EN Signal 6 SD2 Rotatory motor s position 2 EN Signal 7 SK1 Rotatory motor s sensor 1 Drive 8 SK2 Rotatory motor s sensor 2 Drive Auto Hematology Analyzer Service Manual V1 1 System Structure 9 P1 851 Rotatory motor s position 1 10 P2 851 Rotatory motor s position 2 11 12 GND GND 13 SD3 Fluctuating motor s position 1 EN Signal 14 SD4 Fluctuating motor s position 2 EN Signal 15 SK3 Fluctuating motor s sensor 1 Drive 16 SK4 Fluctuating motor s sensor 2 Drive 17 P L1 10ml motor s position 18 P_L2 2 5ml 50ul motor s position 199 20 GND GND 21 SD5 10ml motor s position EN Signal 22 SD6 2 5ml 50ul motor s position EN Signal 23 SK5 10ml motor s sensor Drive 24 SK6 2 5ml 50ul motor s sensor Drive J14 Power Connector PIN Defined Explanation 1 12VP CPU board Power supply 2 PGND PGND 3 7 2V Printer power 4 12VP CPU board Power supply 5 PGND PGND 6 7 2V Printer power J13 12V Connector PIN Defined
83. l V1 1 3 9 Hardware 3 3 Power Drive Board Functions and Modules The power drive board is designed to control the rotatory motor fluctuating motor and syringe motors Besides it also receives the switch signal from the host to control the pump and valve The power board has three modules ON OFF control module motor control module and power supply module The ON OFF control module consists of optical coupler isolation circuit valve driving circuit and pump driving circuit Optical coupler isolation circuit isolate the parallel ON OFF signal coming from the host eliminate the interference of large current signal to the front end control circuit Valve driver circuit realize power driving function for 18 way valves the driving current is 1 5A and the driving voltage is 12V Pump driver circuit realize power driving function for 4 way pumps the driving current of three ways is 1 5A and the driving current of the other way is 3A The driving voltage is 12V Motor control circuit it consists of serial port communication circuit program control circuit current limiting circuit motor driving circuit and protective circuit Serial port communication circuit realize serial port communication with the host the signal level is compliable with RS232 serial port According to the requirement for isolation the communication between the host and the module is isolated by using optical coupler Besides communication management ci
84. lace the rinse diluent lyse sensor or re fix the washer No backlight The inverter is broken Replace the inverter P PLT RBC s result shows Test the RBC aperture voltage is not stability the range is 4 5 11 0V Replace the RBC bath PLT result is always over 1000 The RBC bath is dirty Clean or replace the RBC bath Power on is normal but the screen is black suddenly after Power supply board and CPU board are broken Replace the power supply board and CPU 2 24 Auto Hematology Analyzer Service Manual V1 1 Troubleshooting initiation board Power on is normal but no The CPU board is broken Replace the CPU display no initiation no board response PLT background is abnormal Connecting ground is not Reconnect ground proper or with high voltage wire Pressure error Pressure pump leakage or Replace pump or tubing broken tubing Pressure filters are blocked Replace new filters R Rotatory motor error The tubing connecting sample probe is loose and liquid enters the motor to make the resistance higher Replace mosule the proble RBC bath overflows full and liquid The V11 valve doesn t work or dirty or doesn t work well Clean or replace the V11 valve RBC PLT s result shows Test the RBC aperture voltage is not stability the range is 4 5 11 0V Replace
85. lash U3 RTC U7 WDT U6 and Super I O U30 CPU SDRAM and Flash make up of the basic computer system Flash serves to store BIOS and FPGA configuration data During initialization CPU can read the FPGA configuration data from the Flash in order to set up FPGA By executing the startup program in BIOS CPU can transfer the main program on the DiskOnModule disk into SDRAM and accordingly run the program RTC and WDT together use PC bus CPU may get the current time by accessing RTC RTC has backup battery therefore it can run normally even in the shutdown state WDT is adopted to provide protective function It can forcibly reset CPU when software fails to function Super I O provides external interfaces The parallel port is for connecting printer Serial port 1 may connect bar code scanner Serial port 2 may realize the Auto Hematology Analyzer Service Manual V1 1 3 3 Hardware communication with external computer The keyboard interface is designed to connect standard keyboard A D amp l O Circuit of this part includes A D collection and I O A D sample this board has three pieces of A D chips U37 is a 12 bit A D to sample voltages of WBC HGB and WBC apertures as well as RBC aperture vacuum pressure 5V supply 12V 12V and 48V supplies of analog signal board and 12V supply of power board CPU select channel by using the analog switch on the analog signal board and saves the data sampled by U37 into U40 and
86. loop of the AMP circuit to adjust the zero point and full scale output range of pressure measurement 4 Temperature measuring circuit use thermistor to monitor the environmental temperature The changes of the thermal sensitive resistor is amplified by AD620 and then output to CPU board 3 6 Auto Hematology Analyzer Service Manual V1 1 Hardware 5 Power supply circuit provide 48V DC 120V AC and 12V supplies The 12V supply is realized by using the DC DC module with 5V input The 48V circuit provides constant current source necessary for RBC PLT measurement The 120V AC circuit provides the necessary voltage to zap the apertures 6 Multiplexer circuit supply the function that multiple way signals can be transmit through the same A D channel Use multiplexer to control the sequence of multiple way output signals to CPU board The signal controlling the analog switch comes from CPU board 7 Supply monitoring circuit monitor 48V and 12V voltages Obtain the desired amplitude of the output signal by using resistor to divide voltages Block Diagram of Analog Signal Board The block diagram of analog signal board is shown in figure 3 3 Pressure Module gt gt Vacuum Signal en eee Pressure Signal Hematocyte Signal Module m WBC Signal e u Power Supply RBC Signal Module PLT Signal K 2 WBC HOLE Signal S 12V 12V It RBC HOLE Signal 2
87. menu operation Move the cursor onto Setup Gain option press ENTER to access the Gain screen Print Count Time Password Patient Limits gt Transmission A Count Date amp Time i Sample Mode Gain 2 E Review Auto Clean Time E e Quality Control Reagent Exp Date s ur Setup tg Print Caption pa Ap Service 74 47 Calibration z e Help CD Shutdown Figure 6 3 SR D Gain WNT WHOLE 44 Value Range Factor HGA Blark 120 0 255 100 120 0 255 100 53 0 255 100 43 0 255 4 50V 128 0 255 100 0 seve Erer password to mod y Figure 6 4 Value value of the digital potentiometer of the current channel Range adjustable range of the digital potentiometer Factor rate of change related to the channel gain amplifying multiple when accessing the screen 6 2 Auto Hematology Analyzer Service Manual V1 1 Adjustment WBC whole blood gain of WBC channel in whole blood mode WBC prediluted gain of WBC channel in prediluted mode RBC gain of RBC channel HGB gain of HGB channel PLT gain of PLT channel Auto Hematology Analyzer Service Manual V1 1 6 3 Adjustment 6 3 Gain of WBC whole blood and prediluted Channel Use normal volunteers fresh EDTA K anticoagulant venous blood to set up the gain of WBC whole blood channel and gain of WBC prediluted channel In Count screen analyze anticoagulant venous blood specimens and make the end position of histograms o
88. mputer are different 2 5ml 8 50ul Motor Error Pon gt 91 The position optical coupler is abnormal The driving motor is abnormal The communication wire of the motor is bad connected The 2 5ml syringe or and 50ul syringe is damaged or the resistance of it has increased The 2 5ml syringe or and 50ul syringe is not mounted to the right position 10ml Motor Error mo oboo The position optical coupler is abnormal The driving motor is abnormal The communication wire of the motor is bad connected The 10ml syringe is damaged or the resistance of it has increased The 10ml syringe is not mounted to the right position Rotatory Motor Error AVN Aa 6 The tubing connecting the wipe block is over tightly fastened The position optical coupler is abnormal The driving motor is abnormal The communication wire of the motor is bad connected The motor components loosen or mounted to the wrong position The pin clip loosens if the tubing connecting the sample probe moves randomly or touches the wall when the motor operates Fluctuating Motor Error 1 Ron The position of the optical coupler is abnormal The driving motor is abnormal The communication wire of the motor is bad connected The motor components loosen or mounted to the wrong position The screw lever is not enough smooth DC DC Error 12V Power Error The DC DC component is abnormal The analog signal board is abnormal
89. n requirement Parameter Background Range Unit WBC lt 0 3 x 10 L RBC lt 0 03 x10 L HGB lt 1 g L HCT lt 0 5 PLT lt 10 x10 L Calibration The instrument must be calibrated after disassembling or replacing parts or troubleshooting Further information and procedures are given in HEMATOLOGY ANALYZER Operator s Manual Section 5 Calibration Imprecision Two samples from normal healthy donors were obtained Precision was evaluated by performing 11 consecutive measurements on these blood samples One sample is tested in whole blood mode and another is tested in prediluted mode Calculate the mean coefficient of variation CV of the 10 results except the first sample for the parameters WBC HGB PLT RBC and MCV imprecision Specifications Parameter Level Unit CV WBC 7 0 15 0 10 L lt 2 5 RBC 3 5 6 0 to lt 2 HGB 110 180 g L lt 15 MCV 80 0 110 0 fL lt 0 5 PLT 200 500 109 L lt 5 Auto Hematology Analyzer Service Manual V1 1 9 1 Performance Test Carryover Carryover was determined for the following parameters WBC RBC HGB and PLT Analyze the high level controls for three consecutive times i1 i2 i3 on the HEMATOLOGY ANALYZER and then immediately run background test for three consecutive times j1 j2 j3 Carryover was calculated with the formula Carryover j1 j3 i3 j3 X 100 Carryover Parameter Carryover Unit WBC s0 5 RBC s
90. need not be adjusted Otherwise adjust the value according the WBC histograms until confirming the gain of WBC prediluted Auto Hematology Analyzer Service Manual V1 1 6 5 Adjustment 6 4 Gain of RBC Channel Set the factory calibrating factor and user calibrating factor of MCV to 100 Adjust the digital potentiometer of RBC channel so as to make the difference between the MCV value obtained from controls measurement and assay less than 2 in the Count screen 1 Set the factory calibrating factor of MCV to 100 Access the Password screen and set the password to 5678 Pree Come Time Patari Livets Tranamzzon fi Count Dam Tire Mi Symp Mode Gyn A Review Am Clean Time H Ge Cusity Corts Razg fap Cote 8 Mr ss id Pre Capon V Seite Na Calbraten Ho D Shin renj Figure 6 8 Password Rex woe Wl 15 48 Password s even Press mecen SSA Access the Calibration Manual 100 MINDRAY CO LTD Figure 6 9 Calibration screen set the calibrating factor of MCV to A Count Ui Sample Mode Review fe Quality Control IG Setup Service Calibration e Help b Shutdown id v v v v Manual Calibration Auto Calibration imenu Figure 6 10 Auto Hematology Analyzer Service Manual V1 1 Adjustment roy Wik Brod Catton RF ROE NATTA 200240426 20020426 2002 04 26 2002 04 26 2002 04 26 wo Press 1111 to select tem X to move
91. ng probe assembly is too tight The detectors are broken Replace the two detectors H Hang during initiation Software has some problem Re install the software Initiation stop Power broken supply board is or replace a new Moduleondisk Replace power supply board HGB always alarm WBC bath is dirty Clean WBC bath HGB error and WBC clog The 10ml syringe s piston falls off Re fix the piston Hematology analyzer leakage and vacuum is abnormal The tubing of waste assembly is kink Release the tubing and empty the liquid HGB background voltage is abnormal no chance to make it down WBC bath is dirty or HBG gain is not correct Dip in the WBC bath and adjust HGB gain HGB error HGB background voltage is abnormal 0 3 2v or 4 9 5 0v it s out of acceptable range Adjust the HGB gain or the resistor VR3 amp VR4 which on the analog board to 4 2V 4 6V HGB adjust error HGB background voltage is Adjust the HGB gain abnormal 3 2 3 4v or or the resistor VR3 4 8 4 9v its out of amp VR4 which on the acceptable range analog board to 4 2V 4 6V Initiation time is too long disk on module is broken Replace hard disk disk on module K Keyboard some buttons no response CPU board or keyboard is broken Replace the CPU board or keyboard L LCD screen there is a l
92. nput AC voltage At the same time the input AC supply provides a startup voltage for U2 control chip to control the supply converter U2 controls the on off of the main switch through monitoring the voltage and current of the 12 5V and 5V feedback circuits in order to stabilize the output of 12 8V and 5V In addition the turn ratio of the transformer is used to stabilize the 30V output At the same time this power supply has over voltage and under voltage protecting functions for 12 8V 5V and 30V On the primary side after the power supply becomes stable the transformer will provide the VCC necessary for U2 Block Diagram of Power Supply Board DC DC isolation and conversion circuit 12 8V filter 12V output and output AC input filter and rectification De 5 12V output 30Vfilter and output and conversion circuit DC DC isolation 3 RR 45 V filter and i y output Startup circuit and gt UCC3810 PWM auxiliary power P control circuit v supply I 12 8V feedback zoo zi and over under voltage detection v 5 V feedback and 30V feedback over under voltage and over under Shutdown detection voltage circuit detection Figure 3 8 Auto Hematology Analyzer Service Manual V1 1 Hardware Difference Bet
93. of this stage to finish high pass filter After amplifying the signal to the value within the required range use U11 to realize active RC filter circuit in order to realize low pass filter as well as limit the band width of the signal within the required range At last the circuit outputs the signal that has passing circuit of removing negative pulse and buffer circuit HGB Measuring Module The input signal of HGB circuit is current signal Before amplifying the signal the current signal shall be first converted into voltage signal OP AMP U27 is adopted to construct this circuit The circuit uses classical current voltage converting circuit Resistor is connected into the feedback loop in order to convert current signal into voltage signal A capacitor is parallel connected into the feedback loop to realize low pass filter After converting the current signal into voltage signal input the voltage signal into the voltage AMP stage U28 is used to realize the AMP circuit Adjustable resistor is connected into the feedback loop in order to adjust the AMP multiple Besides a zero adjusting circuit is connected to the input end via an add circuit in order to ensure that when the input is zero the input signal of the circuit has positive voltage An adjustable resistor is used to control the output of zero point Pressure Monitor Module and Temperature Monitor Module Both pressure measuring and temperature measuring circuits use instrumentation ampl
94. oll 30 pages to ext Figure 10 22 Severe interference in PLT channel Data length increases obviously normal sample curet potion 128000 Prese T to show rater r Ji to scroll page TII 10 scroll 50 pages ENTER to ext Figure 10 23 Interference occurs because gain of PLT channel is too large Data length increases background count curent poston 342016 ee oe ea Presa 2 to show ruber f Ji te sorot 1 page TY1 to pmo 30 pages ENTER to ez Figure 10 24 e Interference occurs because gain of PLT channel is too large Data length increases normal sample 10 8 Auto Hematology Analyzer Service Manual V1 1 Histograms and Pulse Graphs p length 1595098 Current posia 239516 Prose 2 so chow rder foJis 10 serol 1 page T1 10 scroll 30 pages ENTER to ext Figure 10 25 e Slight interference in WBC channel Data length does not increase obviously normal sample whe length 420038 curer postion 7168 Press 2 to show ruler femjles to scrot 1 page TI to scroll J pages ENTER to et Figure 10 26 Inadequate or no hemolysis in WBC channel Data length increases whe lengh 4096000 eurrert postna 3072 TUT Frees Z to show ndn 8 seron page Ni 49 96108 50 pages ENTER to ext Figure 10 27 e Slight interference in RBC channel Data length does not increase obviously normal sample Auto Hematolo
95. ood Cattraten Rraov wn TOS 487 Defaut Factor Tene 00 0 1 1 HGS 100 4 100 0 100 0 1 00 1 FI K ma ke EI E OIRIS Figure 2 2 Select Yes store the new factors Select Cancel reserve the old factors Verification After entering the new factors run the calibrator in Count screen Verify that the results are within the specified range eThe Automatic Calibration procedures are 1 Set up the sample mode to Whole Blood or Prediluted 2 Enter the reference value of the calibrator 3 Run the calibrator 4 Confirm the calibration factors Auto Hematology Analyzer Service Manual V1 1 2 11 Troubleshooting A Count Uy Sample Mode Review fe Quality Control I Setup Ap Service amp Calibration Manual Calibration ge Help Auto Calibration A Shutdown m MENU v v v v v MINDRAY CO LTD Figure 2 3 In menu operation move the cursor to Calibration Auto Calibration press ENTER key to access Auto Calibration screen Whole Blood Calitraton wat wou AAOS 27 x 1 Eat Sue No reference data cannot calibraton Figure 2 4 Edit the reference Press 1 to access Edit Reference status Enter the reference values of the calibrator Press T to select the item and gt to move the cursor within the item Press 0 9 to enter numbers The fixed decimal format is adopted so that the user need not enter the de
96. orking If the temperature is less than 15 C it slows the reaction rate from hemoglobin to cyanmethemoglobin This may result in increase of the hemoglobin data It may also result in increase of the WBC count because the RBCs are not sufficiently hemolysed due to the lower temperature Insufficiently hemolyzed RBCs will be included in the WBC count as RBC ghosts Storage for Blood Sample Measure all required parameters soon after sampling the whole blood from a patient As time elapses after blood sampling the blood cells volume and density change The ratios of the volume and density variations depend on the environmental conditions and patient If the blood sample isleft in an air conditioned room for a long time the volume of the red blood cell increases and the MCV RDW and MPV will be affected and moreover the PLT will be easily aggregated WBC part differential To get high reliability on the acquired data measure the blood samples within 6 hours after sampling the whole blood If the blood sample is left in an air conditioned room for a long time geerally the WBC membrane s resistance against hemolysing reagent is decreased Therefore the WBC histogram of the correct shape cannot be obtained Blood Sample from a Patient with Specified Conditions To measure a blood from a patient who has hepatopathy certain special treatments or is a neonate it may be necessary to use a method other than the hematology Analyzer
97. quired CV values are out of the CV specifications the reproducibility of the instrument is poor To troubleshoot the instrument refer to Troubleshooting Erroneous Data of this chapter Auto Hematology Analyzer Service Manual V1 1 Troubleshooting lt Data example and CV specifications gt N X CV CV Specification WBC 10 10 0 1 05 2 5 or less RBC 10 4 23 1 22 2 or less HGB 10 130 0 77 1 5 or less PLT 10 201 3 25 5 or less Lymph 10 140 1 47 Lymph 10 41 0 1 28 Gran 10 4 9 1 77 Gran 10 48 2 1 15 HCT 10 36 1 1 28 MCV 10 86 9 0 24 0 5 or less MCH 10 31 6 1 02 MCHC 10 1353 1 08 N Number of samples for each parameter X Mean of sample data for each parameter CV Standard deviation divided by mean X Note Normally the hematology analyzer counts approx 4 0X10 blood cells for 16 seconds per one RBC counting The reproducibility for the hematology analyzer is statistically determined by the number of blood cells aspirated through the aperture The reproducibility is better as the number of counted blood cells increases the reproducibility is worse as the number of counted blood cells decreases That is the acguired data has more deviation when a blood sample of lower concentration is counted The following explanation and diagram show what the CV values mean for data of each parameter For e
98. rcuit is used to realize multi machine communication management Program control circuit control the rotatory motor fluctuating motor and 10ml syringe motor and 2 5ml amp 50ul syringe motor according to the command from the host Three 53 series CPUs are used to realize the control At the same time receives information of received command and returns the executed results to the host Current limiting circuit use constant current to control the syringe motor and the 3 10 Auto Hematology Analyzer Service Manual V1 1 Hardware fluctuating motor in order to obtain good results Motor driver circuit drive the step motor The driving current of each phase should be 1A Protective circuit Because the motor is inductive load protective circuit should supply continuous current for it Power supply module provide 7 2V and 5V supplies to respectively power of the recorder and the power board Block Diagram of Power Drive Board T v a miler gt o Valve driver gt t v iy Tat gt olation Pimp driver gt t ignal MO board utroll iz purp mt plex gt lat jon Driver reuit A p urrat limitim river R ircult poteet ing b x ning mot IH 3 F CPU of fluctuat ing ts tial port Posi t ton ampimtin am 4 ami rotatory mangement coit notars sensor circuit a a 11 iz Driver gt mui lni protect tne mtor circuit gt PU of 10m1 Posi t bon motor Sensor cirnumn urrent imi tit Driving k gt circuit p
99. rd 3210 after a count you can view the WBC pulse graph of this count by pressing 1 and RBC pulse graph by pressing 2 and PLT pulse graph by pressing 3 Presses ENTER to exit When the instrument is working normally the length of pulse data is related to the concentration of the blood sample The length of the pulse data should be within a limit range For general samples the range should be WBC lt 1M RBC lt 600K PLT lt 1M Data length of abnormal sample will not lie in this range Length of normal level controls data should be WBC 400 700K RBC 250 450K PLT 300 600K 10 2 1 Normal Pulse Graphs e WEC pulse graph of normal sample Figure 10 12 Pulse graph of normal WBC background 10 4 Auto Hematology Analyzer Service Manual V1 1 Histograms and Pulse Graphs whe lengh 14208 current posta 3072 Press 3 to show ruler to smili Figure 10 13 RBC pulse graph of normal sample roc length 319549 Curent ponbon 7680 Figure 10 14 Pulse graph of normal RBC background Figure 10 15 PLT pulse graph of normal sample Auto Hematology Analyzer Service Manual V1 1 10 5 Histograms and Pulse Graphs prleagh soe current postea 1792 UM Trees Z to show nder e to serol page TILI to serol 50 pages ENTER to ext Figure 10 16 Pulse graph of normal PLT background Prece 8 to chow rier eM to perot pa
100. ress down the bar Figure 5 80 Close the door of the recorder Figure 5 81 5 36 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 1 12 Replace Recorder Module Open the door of the recorder Figure 5 82 Screw off the two screws that fix the recorder module The two screws fix the recorder module Figure 5 83 Remove the recorder module using small slotting screwdriver to OT Unclench the two latches to recorder box direction Figure 5 84 Auto Hematology Analyzer Service Manual V1 1 5 37 System Structure 5 2 Disassemble assemble Circuit Boards Figure 5 85 5 38 Auto Hematology Analyzer Service Manual V1 1 System Structure 5 3 Connect Power Supply DE 2 Z J 3 4 5 Figure 5 86 No Socket Plug 1 P7 070950 12V 2 P8 070960 30V 3 P4 071100 FDD 4 P2 07094 5V0 5 P1 07106 AC input Auto Hematology Analyzer Service Manual V1 1 5 39 System Structure P7 12V Connector PIN Defined Explanation 1 GND 12Vp power Ground 2 PGND 12Vp power Ground 3 PGND 12Vp power Ground 4 12VP 12 VP 5 12VP 12 VP 6 12VP 12 VP P8 30V Connector PIN Defined Explanation 1 30GPGND 30V power Ground 2 30GPGND 30V power Ground 3 30VP 30VP 4 30VP 30
101. rotect ing ola 1 mot cirit ct sa nr osi t ton gt 2 Salul n sensor circu BOTOI Figure 3 4 Switch Control Module The control signals of 18 ways valves and 4 ways pumps are transmit to the power board through parallel port 7 optical couplers are used to isolate the digital supply from the driving supply in order to avoid the interference of big current on the Auto Hematology Analyzer Service Manual V1 1 3 11 Hardware front end circuit The circuit driving the valve uses ULN2068B as the driver chip Its driving voltage is 12V The maximum driving current of each valve is 1 5A Similar to the circuit driving the valve the circuit driving the pump also uses ULN2068B as the driver chip Their difference lies in that a pump with big power consumption uses transistor Q1 to amply the current in order to obtain 3A driver current The control logic level is TTL which is valid at low level It means when the input signal is 0 the corresponding pump or valve is connected through Motor Control Module Receive the command from the host via serial port under the control of serial port communication and management circuit For serial port communication circuit the optical coupler isolation OP7 and OP8 as well as level transition U10 are adopted to realize isolation and level transition Besides U28 is applied to manage the communication circuit so as to realize multi machine communication The 4 step motors are controlled by
102. s if the remained regents are not enough the user should change a new one Check the regents expiration date 7 2 Monthly maintenance Use probe cleanser to clean the sample probe wipe block The step is Press MENU button gt Service gt Wipe block cleaning Use sample probe localizer to correct the position of the sample probe The step is 1 Press MENU button B Setup P Password Input password 3210 Service B System test gt Fluctuating Motor to make the sample probe moving up 2 Loose the screw that fix the sample then use localizer to adjust the position of sample probe Auto Hematology Analyzer Service Manual V1 1 7 1 Maintenance Use E Z cleanser or probe cleanser to clean the baths The step is Press MENU button gt Service gt Clean baths Calibrate the unit Refer to Section 5 Calibration of operation manual 7 3 Half year maintenance Replace vacuum filters if necessary should replace the pressure filters Refer to the item of Replace Filter in section 8 Maintenance of operation manual Delete the old stored data The step is Press MENU button B Review 3B Sample Review BPress the button of 5 to delete all of the stored data Clean the valves especially clean the V11 and V12 All of the disassembling and cleaning procedures please refer to the chapter 5 System Structure Clean the two baths and apertures af
103. s Rotatory Motor WBC AD INT Fiuctuabng Motor RBC AD INT Pret PLT AD INT Vacuum WEC Time s Pressure RBC Trne s Pressure WEC Aperture V Vacuum Filter RBC Aperture V Reni sec tem ENTER esem JJ Figure 5 30 1 Direction ep Figure 5 31 5 Press 1 to move the sample probe to the upper position Figure 5 32 5 14 Auto Hematology Analyzer Service Manual V1 1 System Structure 6 Loosen the nut of the fixing screw of fixing the sample probe Figure 5 33 7 Pull up the sample probe until it leaves the sample probe wipe block see figure 5 34 Figure 5 34 8 Then pull off the two inlet tubing of the sample probe wipe block take out the sample probe wipe block and replace it with a new one see figure 5 35 Figure 5 35 Auto Hematology Analyzer Service Manual V1 1 5 15 System Structure 9 When replacing the sample probe wipe block insert the two inlet tubing and clip the sample probe wipe block into the hold plate Pay attention to the corresponding relationship between the tubing and the tubing connector Connect the tubing with flag to the tubing connector at the bottom of the probe wipe block Install the probe wipe block to the original position Connect the inlet tubing with mark to the lower end 10 Insert the sample probe into the sample probe wipe block Insert the localizer of the sampler probe from the bottom end of
104. t malfunction instrument failure damage to the instrument A NOTE A Note provides specific information in the form of recommendations pre requirements alternative goods or supplemental information A WARNING A Potential biohazard 2 A A WARNING ZN ZEN Avoid contacting with the sample probe Auto Hematology Analyzer Service Manual V1 1 i Content Content Ghapteri I Gener alec ss eiessizs isa rec pile see tai odd ja a repki ega 1 1 Tel INtROGUCTION n sszege PA ee ies Secon ves Pole de Radia Moen eee 1 1 1 2 S61VICE POM Y sinji ne r Aig ee sie ed thse Re Mid Aa aa Abeer eis 1 2 153 Spo ITICAUO a EEEE ina iv nebi deli al la Aba 1 3 4 Panel Description zna iska piran an ten an ag Bite ee ude 1 7 1 4 1 Front Panel and Keysi nicit tried wines ines eg s r valo s ge v l ezt dili even 1 7 1 42 Rear Paneli ccivaieie iiss PE EVI een ee eee oan iis 1 9 1 4 3 Front review without front panel 1 11 1 4 4 Right side view without the door 1 12 1 4 5 Left side view without the door 1 13 1 5 Menu Structure Chatt sc scectivscecctet cle censae becca chee aveve ge a bet cece ei eaa eaaa 1 14 Chapter 2 Troubleshooting ee 2 1 2 1 Che k PrO edUTE iss sivine kev kakav del ee igre daha eile eae 2 1 2 2 Check Items before Instrument Check 2 2 2 3 How to Check Sample Data 2 4 2 4 Troubleshooting Erroneous Data 2 16 2 5 TFOUDIESMOOLING E ez se iii ase ej ed PERE A digas RAK
105. tal GND 20 VAL9 Valve Control 21 DVCC Digital GND 22 VAL10 Valve Control 23 PUMPO Valve Control 24 VAL11 Valve Control 25 PUMP1 Valve Control 26 VAL12 Valve Control 27 PUMP2 Valve Control 28 VAL13 Valve Control 29 PUMP3 Valve Control 30 VAL14 Valve Control 31 DGND Digital GND 32 VAL15 Valve Control 33 DGND Digital GND 34 VAL16 Valve Control J4 Rotatory Motor Drive PIN Defined Explanation 1 803 ORANGE Rotatory Motor Drive Signal 2 803 BLUE Rotatory Motor Drive Signal 3 803 YELLOW Rotatory Motor Drive Signal 4 803 BROWN Rotatory Motor Drive Signal Auto Hematology Analyzer Service Manual V1 1 System Structure 803_RED Rotatory Motor Drive Signal 803 BLACK Rotatory Motor Drive Signal J6 Fluctuating Motor Drive PIN Defined Explanation 1 851_WHITE Fluctuating Motor Drive Signal 2 851 YELLOW Fluctuating Motor Drive Signal 3 851_BLUE Fluctuating Motor Drive Signal 4 851_RED Fluctuating Motor Drive Signal J7 10ml Motor Drive PIN Defined Explanation 1 L1_D 10 ml Motor Drive Signal 2 L1 C 10 ml Motor Drive Signal 3 L1 B 10 mi Motor Drive Signal 4 L1 A 10 ml Motor Drive Signal J8 50ul 2 5ml Motor Drive PIN Defined Explanation 1 L2 D 50Ul 2 5ml Motor Drive Signal 2 L2 C 50Ul 2 5ml Motor Drive Signal 3 L2 B 50Ul 2 5ml Motor Drive Signal 4 L2 A 50UI 2 5ml Motor Dr
106. ter disassemble them Please refer to the chapter 5 System Structure Check the stability of HGB background voltage The step is Press MENU button gt Setup Password B Input Password 3210 B Gain B Adjust the HGB value Check the stability of vacuum The step is Press MENU button gt Service gt System Status or System test Vacuum Test all valves work status The step is Press MENU button Service B Valves test Check the status of syringe Clean inside of the eguipment 7 2 Auto Hematology Analyzer Service Manual V1 1 P N 3001 10 07046 3001 10 07047 3001 10 18499 3001 10 13054 3001 10 07050 3001 10 07059 3001 10 07252 3001 20 06898 3001 20 07274 3001 30 18451 3001 20 07072 3001 20 07245 3001 20 07246 3001 30 06860 3001 30 06862 3001 30 06864 3001 30 06866 3001 30 06870 3001 30 06880 3001 30 06889 3001 30 06923 3001 30 06924 3001 30 06925 3001 30 06930 3001 30 06931 3001 30 06957 3001 30 07000 3001 30 07021 3001 30 07131 3001 30 18473 TR6D 30 16662 3001 30 07154 3001 30 07156 530B 10 05275 Spare Part list Chapter 8 Spare Part List ITEM 50ul Syringe 10ml Syringe Rotatory Motor 4S420 12048 Fluctuating Motor 2542Q 05640 Syringe Motor Sample Probe Vacuum Pump START Key Keypad Panel English Display assembly Transformer 3 Way Valve 2 Way Valve CPU Board Power Drive Board Volumetric Metering Board Keypa
107. ting the vacuum chamber is damaged Pressure2 Low 13 14 15 16 Pressure pump has fault Tubing pressure chamber has leaks Tubing connecting pressure chamber to sensor loosens or falls off The valve connecting the pressure chamber is damaged Recorder Out of Paper There are no papers in the recorder box The sensor is abnormal Recorder Too Hot The thermal head is too hot The sensor is abnormal File Error Bar Code Invalid P PMO ID The system software is destroyed The DiskOnModule disk has bad pars The format of bar code inputted is invalid for BC 3000PLUS Bar Code Com Error There are some errors when the bar code scanner communicates with BC 3000PLUS host Printer Error The printer is not connected correctly There are no papers in the printer Wrong type printer which BC 3000PLUS does not support Diluent Expiry Diluent exceeds its Exp date The diluent Exp data inputted is incorrect Rinse Expiry Rinse exceeds its Exp date The rinse Exp data inputted is incorrect Lyse Expiry N rp alpo a Lyse exceeds its Exp date The Lyse Exp data inputted is incorrect Trouble Possible Cause Poor repeatability o Operation is not normalized sample is not completely mixed up Sample is heavily polluted Sample is improperly selected such as the sample has blood clots Wipe block is inaccuratel
108. to return a unit to Mindray the following procedure should be followed 1 Obtain return authorization Contact the Mindray Service Department and obtain a Customer Service Authorization Mindray number The Mindray number must appear on the outside of the shipping container Return shipments will not be accepted if the Mindray number is not clearly visible Please provide the model number serial number and a brief description of the reason for return 2 Freight policy The customer is responsible for freight charges when equipment is shipped to Mindray for service this includes customs charges Company Contact Address Mindray Building Keji 12th Road South Hi tech Industrial Park Nanshan Shenzhen P R China Phone 86 755 26582888 Fax 86 755 26582680 Authorized Representative Name Shanghai International Holding Corp GmbH Europe Address Eiffestrasse 80 D 20537 Hamburg Germany Phone 49 40 2513175 Fax 49 40 255726 iv Auto Hematology Analyzer Service Manual V1 1 Conventions Used in This Manual and Instrument Warnings Cautions and Notes Warnings cautions and notes are used in this manual to alert or signal the reader to specific information A WARNING A Warning alerts the user to the possible injury or death associated with the use or misuse of the instrument A CAUTION Caution alerts the user to possible injury or problems with the instrument associated with its use or problem such as instrumen
109. tor are invalid Maximum 5 samples can be counted for auto calibration The auto calibration result will be displayed only after 3 samples are counted If the calibration factor of a parameter is out the range of 75 125 it will not be displayed find the reason If necessary contact the Mindray Customer Service Department or the distributor Confirm the New Calibration Factors Press MENU to return to the menu operation The dialog box pops up as shown in below figure 2 14 Auto Hematology Analyzer Service Manual V1 1 Troubleshooting EI s Figure 2 8 Select Yes save the new calibration factors Select Cancel keep the original calibration factors Verification When the new calibration factors are adopted count the calibrator in the Count screen and verify that the result is within the specified range Auto Hematology Analyzer Service Manual V1 1 2 15 Troubleshooting 2 4 Troubleshooting Erroneous Data Background Noise When the count data of a container filled with diluent just exceeds the tolerable value of the parameter shown in the table in Background Noise Check of this chapter the seven possible causes of error are 1 2 Dirty diluent container 3 Dirty baths 4 Dirty valves Dirty diluent 1 2 3 4 A noteA Before storing the instrument for a long time clean and empty all fluid tubings in the instrument with distilled water Otherwise the diluent salt adheres to th
110. tors old factor x reference value new factor average of test value Auto Hematology Analyzer Service Manual V1 1 2 9 Troubleshooting Example Reference value of WBC 8 4 In whole blood mode three running values of WBC are 8 1 8 0 8 1 8 1 and 8 3 The mean value of WBC is 8 12 Old calibration factor in the whole blood mode is 98 9 Xx Mean E 8 12 n CV lt 2 E zeke old factor x reference value 98 9 x8 4 _ 102 3 average of test value 8 12 Enter the Calibration Factors In the menu operation move the cursor to the Calibration Manual Calibration and press ENTER to access the manual calibration screen as shown in below figure Press ENTER to access the Edit Parameter state Whole Blood Calbrabon READY WHOLE 08 49 Y y Dis v r lollus 4 l l l T gt O manj Press 1X 1 to select item to move cursor in tern Figure 2 1 Press T 1 to select the item and gt to move the cursor within the item Press 0 9 to enter numbers The fixed decimal format is adopted so that the user need not enter the decimal point 2 10 Auto Hematology Analyzer Service Manual V1 1 Troubleshooting The factor should be within the range of 75 125 Confirm the New Calibration Factors After entering the new factors press MENU key to return to menu operation then the dialog box pops up as shown in below figure Mo Ve Bl
111. ut the specification range the reproducibility of the instrument is poor If the reproducibility is found to be poor this printed result is used to troubleshoot the instrument as described in Troubleshooting Erroneous Data of this chapter Check Procedure 1 Reduce the background noise Refer to Background Noise Check of this chapter 2 Access Quality Control normal level Controls QC Edit File X to set a new control file and input each parameter s specification 3 Access Quality Control Commercial Controls QC Count Count a diluted sample from the same sufficiently mixed hematology control 10 times 4 Access Quality Control Commercial Controls gt QC Graph gt File X to review the result and CV value 5 If you want to print out the displayed values with an built in thermo printer unit press the print key which on the keypad directly in the graph screen or table screen Data Check with Hematology Control The CV Coefficient of Variation indicates the data reproducibility on each parameter A lower CV value for a parameter indicates better reproducibility for the parameter i e each sample data for the parameter deviates less Check the CV value for each parameter by comparing it with the CV specifications as shown in the next page described in the brochure You get the CV value by counting a normal concentration hematology control 10 times consecutively If the ac
112. uto Hematology Analyzer Service Manual V1 1 Hydraulic System 4 4 6 Dispense Diluent a Dispense Diluent 10ml syringe aspirates 1 6ml diluent N 10ml syringe primes 1 6ml diluent to the cup Exit dispense diluent Y y Clean the sample probe CD Figure 4 7 Dispense Diluent Auto Hematology Analyzer Service Manual V1 1 4 9 Hydraulic System 4 4 7 Shut Down Shutdown 10ml syringe aspirates 1 6m E Z cleanser by sample probe from vial Empty WBC and RBC baths Add diluent and 0 8ml E Z cleanser from sample probe to WEC bath Add diluent and 0 8ml E Z cleanser from sample probe to RBC bath Prime the volumetric glass tubes with E Z cleanser from the WBC and RBC baths Add diluent to WBC bath Add diluent to RBC bath 10ml syringe moves to the end position Figure 4 8 Shut Down 4 10 Auto Hematology Analyzer Service Manual V1 1 Hydraulic System 4 5 Hydraulic System Flow Diagram 4 5 1 Inspire Sample and Diluent Whole Blood Mode 13 Figure 4 9 Inspire Sample and Diluent Whole Blood Mode Auto Hematology Analyzer Service Manual V1 1 4 11 Hydraulic System 4 5 2 Inspire Sample and Diluent Prediluted Mode 5 a F a co a y a Cs gt co N zi N gi N S hS VA r T E m a si
113. vacuum pump remove the four fixing screws on the back of the pump and then remove the pump Follow the reversed steps to install the new pump Figures 5 43 Figures 5 44 5 20 Auto Hematology Analyzer Service Manual V1 1 System Structure Figures 5 45 Auto Hematology Analyzer Service Manual V1 1 5 21 System Structure 5 1 6 Replace Count Bath ZN WARNING Potential biohazard Remove RBC bath Remove the fixing screw on the shielding box of the RBC bath Then remove the shielding box Take out the RBC bath assembly from the clips Pull off the tubing connected to the RBC bath and disconnect the connector of the RBC bath inside the enclosure remove the RBC bath assembly Figures 5 46 Figures 5 47 5 22 Auto Hematology Analyzer Service Manual V1 1 System Structure When replacing the RBC bath you must replace the tubing connected to the metal junction Use a nipper to remove the RBC PLT aperture Remove the two screws and then take out the aperture between two washers Figures 5 48 Figures 5 49 A noteA The concave side of the aperture must face to the front bath Remove WBC bath Remove the fixing screw on the shielding box of the WBC bath Then remove the shielding box Take out the WBC bath assembly from the clips Pull off the tubing connected to the WBC bath and disconnect the connector of the WBC bath inside the enclosure remove the WBC bath assembly Auto
114. warranty is limited to repairing at Mindray s option any part which upon Mindray s examination proves defective THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE Exemptions Mindray s obligation or liability under this warranty does not include any transportation or other charges or liability for direct indirect or consequential damages or delay resulting from the improper use or application of the product or the substitution upon it of parts or accessories not approved by Mindray or repaired by anyone other than a Mindray authorized representative This warranty shall not extend to any instrument which has been subjected to misuse negligence or accident any instrument from which Mindray s original serial number tag or product identification markings have been altered or removed or any product of any other manufacturer Safety Reliability and Performance Mindray is not responsible for the effects on safety reliability and performance of the BC 3000plus Hematology Analyzer if MM assembly operations extensions re adjusts modifications or repairs are carried out by persons other than those authorized by Mindray m Personnel unauthorized by Mindray repairs or modifies the instrument Auto Hematology Analyzer Service Manual V1 1 iii Return Policy Return Procedure In the event that it becomes necessary
115. ween Two Types Power Supply Board The difference between the two types power board is the rectification circuit The figure 3 9 is the 220V input rectification circuit and the figure 3 10 is the 110V input rectification circuit The difference on PCB layout is if there is a connection wire between BD1 and the joint of C5 and C6 as shown in figure 3 10 BD1 Ll cs ce E x S0UIOOV JE KBUSK Ra S 2 o 1 R5 2K2W RIH 22KW Figure 3 9 Connection Wire pa OKAWO 360K L 2W ta R14 maka Figure 3 10 Do not confuse the type of the power supply board otherwise lead to the instrument damage or body hazard Auto Hematology Analyzer Service Manual V1 1 3 19 Hydraulic System Chapter 4 Hydraulic System 4 1 Hydraulic System Block Diagram 18 Figure 4 1 Hydraulic System Block Diagram Auto Hematology Analyzer Service Manual V1 1 4 1 Hydraulic System 4 2 Units of Hydraulic System M Sensor Unit This part is made up of front bath aperture rear bath electrodes and seal washer This part is the most important for the whole instrument Simply speaking this part is a sensor Most measuring results are generated by this part Mm HGB Unit This part is used to measure the hemoglobin concentration M Dilute Unit This part consists of 4 step motors rotatory motor fluctuating motor and two syringe driving motors 2 syringes 10ml and 50ul wipe block and sample probe It is us
116. xample when noting the RBC data on the printout see the previous table the mean of the RBC data is 4 43 and CV is 0 8 0 8 of 4 43 is 0 035 Therefore the range is 4 395 to 4 465 4 430 035 This means that six of the ten acguired RBC data are within the range Auto Hematology Analyzer Service Manual V1 1 2 7 Troubleshooting Accuracy Check 2 8 2 data 4 4 395 4 465 _ gt 4 37 4 39 14 40 4 43 4 44 445 445 4 45 4 461 4 47 4 48 6 data 2 data This check is used to check the accuracy of the measurement by comparing the actually measured data of the hematology control with expected value on the assay sheet of the hematology control If there is a large difference between them calibrate the instrument by resetting the calibration coefficient for each parameter Check Procedure 1 Gently take the hematology control out of the refrigerator and place it in a normal temperature environment for a while to raise it to room temperature The hematology control must be within the expiration data 2 Confirm that the hematology control is not hemolysed Normally the hematology control is separated into blood plasma and blood serum of the hematology control may be mixed Also if the hematology control is frozen it is hemolysed 3 Measure each parameter with the hematology control 4 Check that the obtained sample data for each parameter is within the range between the lower and upper expected values on the
117. y Brightness nena 6 12 6 8 Adjust Vacuum and Pressure nn 6 13 2 Auto Hematology Analyzer Service Manual V1 1 Content 6 9 Adjust Gount time wits veiet celica din cis anaes dade 6 14 6 10 Adjust Auto Clean Time 6 16 6 11 Adjust Volumetric Metering Board n 6 17 6 12 Re calibrating Instrument 6 19 Chapter 7 Maintenance en 7 1 7 1 Daily MAINCOMNANCE iiceoe neniani andaa aa aiaa ana ia Ea e aa aa iaaa aaa a iaaii 7 1 72 Monthly maintenance munh nite eee inde Senet 7 1 7 3Half year maintenance 7 2 Ghapter 8 Spare Part LIST ii ccd ee ned ae dir pe e KE value jo 8 1 Chapter 9 Performance Test ea 9 1 Chapter 10 Histograms and Pulse Graphs 10 1 LOS AFISTOGFAMS A tati ana na NA ta lds ete Viki 10 1 10 2 PulSe Grapns sei esi esaat re EEA ei ETRE meinen 10 4 10 2 1 Normal Pulse Grapns iria e a akie 10 4 10 2 2 Abnormal Pulse Graphs sssesesssssssrrrnssrrnrssrrnnnsrnrnnarannternnnsentnntnennnnttenanaennnne 10 6 Chapter 11 Password and Upgrade software 11 1 4 1 o ELE OLAS MPE EEE E ATA EE eaves 11 1 11 2 Upgrade System Software 11 2 Appendix fied kt eee iw ede en A a e een A SEL A 1 Hardware Diagram of BC 3000PLUS nn A 1 Hydraulic Diagram of BO 3000PLUS e na A 2 Auto Hematology Analyzer Service Manual V1 1 3
118. y positioned Instrument has interference Reagents are polluted or exceed their Exp date Severe interference ro Os Shielding box of sample bath is not well mounted Shielding wire of analog signal board is not connected correctly Auto Hematology Analyzer Service Manual V1 1 Troubleshooting Valve V11 or V12 connecting count bath is not well closed or has dirt The gain is set incorrectly The input power is unstable Power off during running a Unstable input power it exceeds the protective range Auto Hematology Analyzer Service Manual V1 1 2 31 Chapter 3 Hardware The instrument has the following hardware CPU Board Power Drive Board Analog Signal Board Keypad Recorder Board Volumetric Metering Board Power Supply Board Display Screen Linear Transformer Hardware Auto Hematology Analyzer Service Manual V1 1 3 1 Hardware 3 1 CPU Board Function and Modules The board consists of three modules Computer System Module With CPU as the core the computer system module also includes some peripheral circuits like RTC WDT SDRAM Flash and Super I O The super I O circuit uses Super I O chip as the core and includes other sub circuits like keyboard serial port parallel port floppy disk drive interface and IDE interface CPU SDRAM and Flash construct the basic computer system and the basic environment for the operation of software

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