Research Pro User Manual 1999
Contents
1. Direction Aspirating dispensing Speed Battery symbol Flashes when the battery is low Left hand display field Operating mode Appears during editing Number of fixed volume FIX or program Program Press briefly to enter exit program level If PROG is in display Speed Option Volume are frozen Speed Display of speeds ated and in by pressing key repeatedly Change by pressing EZ Select rocker This switch can be pressed upwards or downwards Selection of basic modes Ejector Actuate key PIP Pipetting Sec 5 1 5 7 eee ieee DIS Dispensing Sec 5 9 dispensing FIX Fixed volume liquid for pipetting Sec 5 8 After E Selection in RE the program level Sec 5 10 po Eom To switch on Press any key firmly To switch off Automatically after 10 min of non use Right hand display field Options Appears when the pipette is callbrated by the user Appears when Reset is requested RESET key AN Liquid can be dispensed during a Reset When the piston is moving Emergency stop Option For PIP and FIX the options are selected by pressing the key repeatedly Display blank Standard operation Sec 5 3 BLO BLOW separate blow out Sec 5 4 RNS RINSE Rinsing mixing Sec 5 5 RP REVERSE PIPETTING Sec 5 6 MAN Manual Sec 5 7 Volume rocker for changing the volume For FIX To select the fixed volume The rocker can be pressed upward2. Care and maintenance Do not clean the display or any of the labeling using acetone or aggressive solutions Do not allow any liquid to enter the device Repairs may be carried out by authorized service personnel only Use original spare parts and accessories battery pipette tips only Battery Charge up the battery before operating the device for the first time see Sec 3 Charge the battery in the pipette only using the charging adapter or the charging stand and the original power unit Ifa flashing battery symbol appears in the display stop dispensing and charge the battery When changing the battery do not allow the battery to come into contact with any metallic surfaces Dispose of used batteries as special waste in accordance with legal stipulations to this effect Never charge the Research pro without a battery Before storing the Research pro for a long period be sure to remove the battery 2 Technical data Volume range Step size Color Pipette tip Test volume Inaccuracy Imprecision ul ul code pl Single channel 0 5 10 0 01 gray 20 u 1 2 5 lt 1 8 10 1 0 lt 0 4 5 100 0 1 yellow 200 u 10 2 0 lt 1 0 100 0 8 lt 0 2 20 300 0 5 yellow 300 u 30 2 5 lt 0 7 300 0 6 lt 0 2 50 1000 1 blue 1000 p 100 3 0 lt 0 6 1000 0 6 lt 0 2 100 5000 10 violet 5m 500 3 0 lt 0 6 5000 0 6 lt 0 15 Multi channel 0 5 10 0 01 gray
3. 5 6 Reverse Pipetting RP RP is recommended for solutions with a high viscosity or with a slightly higher vapor pressure Reverse Pipetting is liquid aspiration with a blow out The liquid is dispensed without a blow out If Filtertips are used please observe the volume restriction Sec 5 11 Programming Select p om e S DOR LC 1 Option Option tion q SS r Procedure O O Aa Qu e ME EOL If PROG appears in the display Press the 225 key briefly until PROG disappears Volume selection here 800 ul The volume rocker has two pressure points for up and down If necessary press the Option key several times RP is selected Aspiration of liquid with blow out If appears in the display Press the key briefly Liquid is dispensed Blow out is discarded If necessary replace the tip Pipette is once again ready to aspirate liquid with blow out 5 Operation 5 7 Pipetting with the MAN option Note The MAN manual option is the equivalent of operation with a mechanical piston stroke pipette Liquid is aspirated and dispensed only when the Actuate key is held down The volume display increases the number of applications available in comparison to a mechanical pipette 1 Aspirating Measuring a small unknown amount of liquid via aspiration into the pipette tip If necessary dilute the liquid to the defined end volume by aspirating additional liquids Liquids can be separat
4. Como programa la dispensaci n est protegida contra posibles cambios Por favor respete las limitaciones de volumen para Filtertips ver cap 5 11 Programming Programaci n Program PROG in the display PROG en el visualizador Select Select the memory slot Seleccionar el puesto de memoria Program Hold down the key until EDIT O icon Ape Pulsar largo la tecla hasta que aparezca EDIT Select lala Select DIS ENTER O Seleccionar DIS Part B Programs Parte B Programas or o Select the dispensing volume Volume Select Tc ENTER The max number of dispensing BIS a A Ea RS steps appears in the right hand side of the display Seleccionar el volumen de dosificaci n El n mero m x de pasos de dosificaci n aparece a la derecha del visualizador Select Select the speeds em I Anu 0 eleccionar las velocidades 42 PROG 12345 Reduction of dispensing steps Is 10 ENTER A Capri Reducci n de los pasos de dispensaci n Select The dispensing procedure is described in Sec 5 9 El procedimiento de dispensaci n se describe en el cap 5 9 IV SD Sequential Dispensing Dispensaci n secuencial This program can be used when different volumes of a solution have to be dispensed consecu tively Up to 20 dispensing steps can be defined If the sum of the dispensing steps is larger than the volume of the pipette ti
5. Instruction Manual Mode d emploi Manual de Instrucciones Contents Sommeire Indice Contents Sommaire Indice Part A Section A Parte A Instruction Mill to aan ion AAEREN R ESANEAN hM Ra Rak dreh gae 2 Mode dela A ad 30 Manual de Instrucciones cuina e e A 58 Part B Section B Parte B Programming fProgramas an nnnn ann REACER in dada SATER NN 86 Ordering Information Informaciones para pedido urssernennnnnnnneennnennnnnnnen cnn ccnn nana 100 eppendorf and eppendorf Research are registered trademarks Registered trademarks are not marked in all cases with TM or in this manual Research pro pipettes are manufactured under U S Patent No 4 671 123 4 905 526 5 187 990 No part of this publication may be reproduced without the prior permission of the copyright owner Copyright 1999 Eppendorf Netheler Hinz GmbH Hamburg Part A Contents 3 1 3 2 3 3 4 1 4 2 5 1 5 2 5 3 5 4 5 5 5 6 5 7 5 8 5 9 5 10 5 10 1 5 11 5 11 1 5 11 2 6 1 6 2 Safety precautions and applicational limitations cciicccic2 eee 3 Handling riva oa 3 Care and maintenance aT Delivery package akei Inserting the A NN 5 Charging the battery nenn anne Operating principle Display and keypad Unit s functions Ejector with extension Hook on the hand resSt Contact surface on the rear of the pipette Acoustic
6. The dispensing liquid is aspirated into the pipette tip When the 200 ul tips are used together with the 300 ul pipette and when Filtertips are used the volume restriction option must be activated in the device parameters Sec 5 11 uo The liquid which is to be aspirated is taken from a suitable vessel When multi channel pipettes are used the reagent reservoir vessel is recommended Before commencing pipetting activities with multi channel pipettes turn the adapter so that it is facing in the same direction as the arrows on the adapter During rotation a small click indicates that the adapter is positioned correctly In addition to the description found in the subsequent sections 5 3 5 11 the following general procedure takes place Attach the pipette tip from the rack Slight force may be used if necessary With multi channel pipettes the pressure on the outer pipette tips is increased by tilting the pipette slightly When aspirating liquid immerse the pipette tip vertically and as little as possible into the liquid Following aspiration remove the pipette tip from the liquid after the acoustic signal has been emitted If air bubbles have been aspirated this process must be repeated Never lay the pipette down when the tip is filled To dispense liquid position the pipette tip against the side of the aspirating vessel as shown in the illustration Depending on the type of problem carryover contaminati
7. an internal correction of the volume is possible The actual volume can be checked via weighing see Sec 2 Technical data Mean value of weighings Actual volume _ _ ______ Density of liquid at weighing temperature 1 If the volume selected display volume is the same as the actual volume no correction is necessary If there is a difference between the actual volume and the display volume the following questions must be answered with no to enable calibration to take place Does the pipette leak s there a temperature gradient between pipette and solution e Was a pipetting option chosen which was not optimal s the balance used to weigh the sample exposed to vibrations drafts and similar influences Is the value for density at weighing temperature incorrect Were tips used which were not original eppendorf pipette tips If the place where the pipette is used is at extremely high altitude an adjustment must be made in line with the ambient air pressure At 1 000 m above sea level there is a volume error of approx 0 4 5 Operation The internal volume correction of the Research pro uses the following formula Actual volume m Stroke movement b 2 m is the definition of the gradient and b is the definition of the axis section of this equation 2 When the device is delivered m 1 000 and b 0 0 ul If this data is changed the CAL symbol appears in the bottom
8. is also permitted N Do not allow any liquid to enter the pipette After that only the lower part of the pipette should be rinsed with distilled water and dried The pipette may be wiped clean with 60 Isopropanol The visible O rings in the lower part of the multi channel Research pro should be lubricated lightly with silicone grease and then wiped with a lint free cloth The procedure for replacing defective O rings when the pipette tips fit incorrectly is described in Sec 6 3 If the pipette is severely contaminated or if very aggressive chemicals are dispensed the lower halfofthe Research pro should be disassembled see Sec 6 2 forthe single channel model and Part B Ordering information for the multi channel model The individual parts are rinsed in distilled water and then dried The piston is then lightly lubricated using silicone grease After opening the pipette please check that the piston is correctly positioned A motor reset must also be executed The motor reset is triggered by holding down the Reset key until R appears in the display 6 2 Sterilization Only the lower half of the pipette can be steam autoclaved 121 C 1 bar 20 minutes The autoclaved parts must be allowed to dry at room temperature The entire lower part of the multi channel model can be autoclaved as one unit With the single channel model the individual parts of the lower part must be disassembled and autoclaved as separate pieces Lower par
9. 20 u 1 5 0 lt 3 0 10 2 0 lt 0 8 5 100 0 1 yellow 200 u 10 2 0 lt 2 0 100 0 8 lt 0 25 20 300 0 5 yellow 300 u 30 2 5 lt 1 0 300 0 6 lt 0 25 50 1200 5 green 1 25 m 120 6 0 lt 0 9 1200 1 2 lt 0 3 The technical data is valid only when the Research pro is used with Eppendorf pipette tips The multi channel data is valid for eight and twelve channel pipettes The 50 1 200 model is available as an eight channel version only Test conditions in accordance with DIN 12650 for piston stroke pipettes with an air cushion by means of a standardized fine balance with a moisture trap Number of determinations 10 degassed distilled water 20 C 25 C 0 5 C maximum speed standard operation prewetted pipette tip dispensing onto inside of tube due to possible evaporation check volumes lt 10 ul immediately after aspiration Battery Nickel metal hydride battery pack 1 200 mAh 2 4 V with reversible overcurrent and overtemperature protection Charging time approx 9 5 hours for a fully discharged battery Overcharging protection with the pipette Power unit Input voltage country specific 230 V 10 50 60 Hz 115 V 10 60 Hz 100 V 10 50 60 Hz Output voltage 9 V DC 200 mA 1 8 VA Technical specifications subject to change N exp PpIUpeL 3 1 Delivery package The Research pro is supplied as a single channel eight channel or twelve channel pipette for different vol
10. 96 480 unid 10 ul 2249 030 3 100 ul 2249 032 0 250 ul 2249 035 4 1000 ul 2249 034 6 Part B Ordering information Parte B Informaciones para pedido Additional accessories Otros accesorios Spare Ni MH battery 1 200 mAh Repuesto bater a Ni MH 1 200 mAh 2246 701 8 Silicone grease Grasa de silicona 2234 851 5 Reagent reservoir autoclavable multi channel reagent attachment 1 set 10 tubs 10 lids 2226 580 6 Dep sito para reactivo toma reactivo autoclavable multicanal 1 set 10 tubos 10 tapas Lid of battery compartment Tapa compartimento de pilas 2246 702 6 Wrench not for 5 000 ul Llave no para 5 000 ul 2247 658 1 Disassembly aid 5 000 ul Ayuda para desmontaje 5 000 ul 2247 660 2 Tools for multi channel model Herramientas para el modelo multicanal 0 5 10ul 2245 689 0 5 100 yl 2246 703 4 20 300 ul 2245 691 1 50 1200 ul 2246 704 2 Spare parts for single channel pipettes Piezas de repuesto para pipetas monocanal The disassembly procedure can be found in Sec 6 2 Para el desmontaje mirar el cap 6 2 Ejection sleeve Eyector Piston Pist n 0 5 10 pl 2247 649 1 0 5 10H 2246 705 1 5 100 yl 2247 649 1 5 100u 2246 706 9 20 300 ul 2247 650 5 20 300u 2246 707 7 50 1000 ul 2247 652 1 50 1000 u 2246 708 5 100 5000 ul 2247 654 8 100 5000 u 2246 709 3 1 Lower part incl seal Sealing inside lower part See next page Change service parts see next page 1 Parte inferior incl junta J u
11. SIGN S csssnreseeseenineeikennnnunen Operation Mode of operation Essential operating information Aspirating and dispensing speeds Loading Gelson oris ak Pipetting in the standard MOMVEL icicc cici iiic ciiiiiiiiiiiic ricin narrar rar rr 10 Pipetting with separate blow out Blow BLO iiiiiiiiiiiiiiiiiiiiiiii isine 11 Pipetting with separate rinsing Rinse RNS Reverse Pipetting RP certain een Pipetting with the MAN 0ptiof saan ra dnem 0 R tienhaara n Pipetting with a preselected fixed volume FIX DISPENSING DIS ae idad Notes onthe prog MSc wana euren Example for programming pipetting in the program level Device parameters nennen ek Changing the calibration CAL cece eee eet rara r rar r inici ani rra Information on initialization INI Sterilization Lower part of multi channel model usssernsnnnnnnnneennnnnnnnnnennnnnnnnnnneennnnnnnnn nen Lower part of Single channel model useersennnennnnennennnnnnnnnnennnnnnnnnnne ernennen Maintenance m Troubleshooting accionar rr Safety precautions and applicational limitations 1 Safety precautions and applicational limitations Before using the Research pro for the first time please read the entire operating manual To guarantee problem free safe operation of the pipette it is essential to observe the following points Handling Use the Research pro only when a pipett
12. a as zug CS If PROG appears in the display Press the 2257 key briefly until PROG disappears Volume selection here 800 ul The volume rocker has two pressure points for up and down If necessary press the Option key several times BLO is selected for blow out Liquid is aspirated If O appears in the display Press the key briefly Liquid is dispensed Blow out Delayed dispensing of residual liquid The pipette is now once again ready to aspirate 5 5 Pipetting with separate rinsing Rinse RNS Rinsing RNS is recommended for sample volumes below 10 ul RNS is suitable for mixing the dispensing volume and the specimen liquid when large volumes are used It is strongly recommended to use the max speed setting Programming If PROG appears in the display Press the 25 key briefly until 2 PROG disappears Volume Volume selection here 5 ul The volume rocker has two pressure points for up and down If necessary press the Option Option eb ation m PP sf key several times RNS is selected for rinsing Procedure 4 Liquid is aspirated If appears in the display Press the key briefly 4 Rinsing mixing takes place three O times after liquid has been 14 dispensed pressing the key when device is Y Return to basic position by not immersed in the liquid The pipette is now once again ready to aspirate 12 uonesedo Operation 5 Operation
13. dispensaci n Volume of the last dispensing step Volumen ltimo paso de dispensaci n Select the speed for all dispensings Seleccionar la velocidad de todas las dispensaciones Programming is complete EDIT disappears La programaci n est completa EDIT desaparece Part B Programs Parte B Programas Procedure Ejecuci n O ej oj O v N PROG 12348 A v N 2 a PROG 12543 A o e Supers 1 F The necessary or largest possible amount of liquid is aspirated If appears in the display press D Absorci n de la cantidad de l quido necesario o m ximo posible Si aparece en el visualizador pulsar lt The first step is dispensed Dispensaci n del ler paso The second step is dispensed Dispensaci n del 2 paso Blank step with special acoustic signal Paso vac o con se al ac stica especial Blow out is discarded No dispensing step Aspiration then occurs for the next dispensing steps or for repeating the first dispensing step Descarga de la carrera excesiva Sin paso dispensador Absorci n para los pasos siguientes de dispensaci n o para repetir el ler paso dispensador 92 apod Programs 93 Part B Programs Parte B Programas V ADS Automatic Dispensing Dispensaci n autom tica When microtiter plates are filled a specific amount of liquid must often be dispensed in rapid succe
14. n de los pasos de dispensaci n Part B Programs Parte B Programas Select Procedure Ejecuci n O O O PROG 12 AIS PROG 12345 A HE O ENTER Dispensing frequency Delays between dispensings 0 1 to 10 seconds Frecuencia de dispensaci n Retardo entre las dispensa ciones 0 1 a 10 segundos Programming is complete La programaci n est completa Aspiration of liquid If appears in the display press Absorci n de l quido Si aparece en el visualizador pulsar Dispensing is carried out while the Actuate key held down An acoustic signal is emitted after the dispensing step Interruption Release the Actuate key Dispensaci n manteniendo pulsada la tecla liberadora Se emite una se al ac stica despu s del paso de dis pensaci n Interrupci n Soltar la tecla liberadora Dispensing is complete La dispensaci n est completa Blank step with special acoustic signal Paso vac o con se al especial ac stica 94 apod Programs Part B Programs Parte B Programas o el VI DIL Diluting Diluir Blow out is discarded No dispensing step Descarga de la carrera excesiva Sin paso dispensador In this case the reagent or diluent and the sample are aspirated together into a pipette tip A pipetting option can be used for dispensing both liquids Aqu se absorben el reactivo o
15. ul 100 2500 50 150 2350 4850 pipeta 1200 pl 50 600 12 36 480 1138 El volumen m ximo de 1000 ul 50 500 10 30 470 940 muestra s lo puede 300 pl 20 150 3 9 141 277 Usarse con el volumen 100 ul 5 50 1 3 47 94 minimo de diluyente y 10 ul 0 5 5 0 1 0 3 4 7 9 4 viceversa VII SDI Serial Dilution Diluir en serie In this program pipetting is linked up to a specified number of mixing cycles The program is used with a specimen liquid The pipetting liquid sample is mixed together with the specimen liquid En este programa el pipeteo va unido a un n mero espec fico de ciclos de mezcla El programa se usa con un l quido ya preparado El l quido a pipetear muestra se mezcla con el l quido ya preparado Programming Programaci n Program p PROG in the display Y SN D PROG en el visualizador Select ZA Select the memory slot ES Seleccionar el puesto de memoria Program Hold down the key until EDIT Pulsar largo la tecla hasta que aparezca EDIT Part B Programs Parte B Programas Select or o Select Volume E Select Select Select Select Procedure Ejecuci n O O PROG 12345 SII 250 PROG 12345 5 0 BLO PROG 12345 Select SDI Seleccionar SDI Volume of sample Volumen de la muestra Aspirating and dispensing speed Select a high speed Velocidad de absorci n
16. users to work rapidly and with a low electricity consumption the highest possible speed for liquid aspirating should be used Depending on the task at hand gels can be loaded in the Standard mode Sec 5 3 or by using the BLO Sec 5 4 or MAN Sec 5 7 options 5 Operation 5 3 Pipetting in the standard mode In the Standard mode blow out is executed automatically when liquid is dispensed The standard mode is recommended for rapid series pipetting with aqueous solutions Programming Volume Option DD ation Pipetting procedure O O uope Bdo If PROG appears in the display Press the 228 key briefly until PROG disappears Volume selection here 800 ul The volume rocker has two pressure points for up and down If necessary press the Option key several times In the Standard mode the display remains blank in this case Liquid is aspirated If appears in the display Press the key briefly Liquid is dispensed with blow out The pipette is now once again ready to aspirate 10 Operation 5 4 Pipetting with separate blow out Blow BLO BLO is recommended for use with liquids with a high wetting power or with liquids which are prone to the formation of foam e g solutions containing protein The residual liquid is dispensed by pressing the Actuate key a second time Programming Select E Volume Option S stion Procedure O O O 11
17. Select rocker PROG appears in the display by pressing the Program key You are now in the second level Using the Select rocker select from the five available program slots Sec 5 10 This level can be quit by pressing the Program key briefly PROG disappears from the display Please refer to the following illustrations when going through each operation AN An Actuate key which has An Actuate key which has been O been pressed briefly is light e held down is dark If the piston movements appear in the display without any volumes this indicates a process which is not connected to liquid aspiration or dispensing Aspirating and dispensing speeds The selected speed can be viewed by pressing the Speed key several times The speed is changed as follows Speed Select Display and selection of D ZOM aspirating speed Speed Select Speedx k Display and selection of O PO dispensing speed This procedure does not have to be completed using the Speed key Any key with the exception of Reset may be used Atthe same time pressing the key executes the respective key function The direction of the arrow for speed flashes in this input field The speed can be altered before every dispensing or aspiration Important High speeds amauuemeas have a much lower current consumption than low speeds Loading gels With the 10 ul and 100 ul Research pro the lowest speeds for liquid dispensing w are ideal for loading gels To enable
18. absorci n del diluyente Sum of sample diluent Selection of dispensing speed Suma de muestra diluyente Selecci n de velocidad de dispensaci n Pipetting options BLO RNS or Standard Opciones BLO RNS o est ndar Diluent is aspirated If appears in the display press D Absorci n del diluyente Si aparece en el visualizador pulsar Air bubble is aspirated The volume is calculated by the program Se ha absorbido una burbuja de aire El programa calcula el volumen Sample is aspirated Se absorbe la muestra Sample air diluent are dispensed Please observe the pipetting option Dispensaci n de muestra aire diluyente Por favor observar la opci n de pipeteo 96 apod Programs Part B Programs Parte B Programas Volume table Diluting DIL Sample ul Air ul Diluent ul The total volume of the Pipette min max min max min max pipette cannot be 5000 ul 100 2500 50 150 2350 4850 exceeded 1200 ul 50 600 12 36 480 1138 The maximum sample 1000 ul 50 500 10 30 470 940 volume can only be used 300 ul 20 150 3 9 141 277 with the minimum diluent 100 ul 5 50 1 3 47 94 volume and vice versa 10 ul 0 5 5 0 1 0 3 47 9 4 Tabla de vol menes Diluci n DIL Muestra ul Aire ul Diluyente ul No puede excederse el Pipeta min max min max min max volumen total de la 5000
19. ain a Stop the recharging process FHT Error 1 battery immediately and insert a battery into the pipette Pipette was inserted into or LUN removed from the charging adapter or the charging stand Display is dark Pipette is in the Sleep mode The pipette does not contain a battery The battery is completely run down The battery also runs down during periods of non use Press any key to switch on the pipette Insert the battery into the pipette Recharge the battery The piston movement was stopped by pressing the ED key emergency stop A request to reset appears in the display Eject the pipette tip When the key is pressed a second time the piston returns to its basic position R O The piston in the pipette must be moved to the basic position requested Press the key Caution As the piston is moving liquid may be dispensed VOL Appears when Actuate key is pressed The volume cannot be aspirated because volume restriction is active Select the volume within a restricted range Filtertips 100 ul tip Alternatively cancel the volume restriction Sec 5 11 Any other error message which appears in the display is only for error diagnosis by the Service Department If the error message does not disappear after a long Reset Motor reset or after the battery has been removed and reinserted see Sec 3 2 ple
20. ase contact the Service Department Additional information When the battery is inserted the program version and the data record 28 appear in the display of the Research pro 4860 ng Troubl 29 7 Troubleshooting Error Cause Solution Pipette is dripping the volume dispensed is incorrect The tip may be loose There may be foreign matter between the pipette and the tip The piston nose cone and cylinder may be damaged or contaminated The lower part may be loose Attach the tip firmly Make sure that an Eppendorf tip is used Wipe the pipette Ensure that the tip is protected from dust Treat the lower half of the pipette as described in Sec 6 and Part B Ordering Information For multi channel model service parts The O ring may be damaged Residual liquid is in the tip See above See above dispensing is incomplete The dispensing speed may The procedure for be too high checking the function is The incorrect pipetting option may have been selected described in Sec 5 Operation Motor stops during the dispensing procedure The battery may be run down The pipette may be heavily contaminated Discard the dispensing as it is incorrect Recharge the battery Sec 3 3 or treat the pipette as described in Sec 6 Part B Contents Parte B Indice PrograMS une nnn I ntroduction ansseseeneae nenn ESCO R anden ll SP Sequential Pi
21. cy and the life of the pipette We do not honor any warranty or accept any responsibility for damage resulting from such action Avisos importantes a las informaciones para pedido S lo se suministran piezas dotadas de n mero de pedido Por favor usar s lo los accesorios originales de Eppendorf La imprecisi n la incorrecci n y la vida de la pipeta puede verse reducida con el uso de otras piezas que no est n recomendadas por nosotros Declinamos todo tipo de garant a y de responsabilidad por los da os que resulten de ello 104 oppad erd SSUODELLUIOJU UORPLULIO JU G6ULBPRIO ing information Informaciones para pedido 105 Part B Ordering information Parte B Informaciones para pedido Change Service parts multi channel Cambio piezas de servicio multicanal Pressure piece Pisador Piston seals J unta del pist n 1 Using the screwdriver press in the catch and pull off the housing 2 Using the tool lever off the metal clips from the safety hook This part is under spring tension Hold the part as shown in the diagram and loosen the metal clip on the other side 3 Pull off the tip holder nose cones Caution The uneven loading of the spring in the pistons is deliberate Note the spring loading and observe these notes during reassembly Before cleaning the piston with 60 isopro panol pull off the springs Lightly lubricate the piston using silicone grease 4 Loos
22. diluyente y la muestra junto con una boquilla de pipeta Puede usarse una opci n de pipeteo para dispensar los dos l quidos Programming Programaci n Program a a lt PROG12345 Select p Program PROG 12345 Select an Eom PROG 12343 or o Select OPA pl PROG Select o GPL 200 PROG 12345 95 Te O ENTER ENTER ENTER PROG in the display PROG en el visualizador Select the memory slot Seleccionar el puesto de memoria Hold down the key until EDIT appears Pulsar largo la tecla hasta que aparezca EDIT Select DIL Seleccionar DIL Sample volume Max 50 of the tip volume Volumen de muestra Max 50 del volumen de la boquilla Aspirating speed for sample Velocidad de absorci n de la muestra Part B Programs Parte B Programas or o Select Volume Select Select Select Procedure Ejecuci n el e eje PROG lt Ea NL 608 d PROG 12345 um SIM BOD vt PROG 12345 Min JIL 6084 HIR ep PROG 12345 SAL 200 PROG 12345 lt SLM BOD PROG 12345 O ENTER O ENTER O ENTER SLM 80 F e ENTER EDIT PROG 12345 A Diluent volume Min 47 of the tip volume Volumen de diluyente M n 47 del volumen de la boquilla Aspirating speed of diluent Velocidad de
23. e next line appears in the display If a device parameter is quit using Reset the setting which was made prior to change is valid Device parameters are called up as follows a With the pipette switched on Speed Option IE on press both keys briefly at the d same time Select E To switch off on acoustic signal S ER Os oem p Select To switch on off on second 4 O ENTER acoustic signal 22 on 23 5 Operation a To view calibration data CAL 00 b O ENTER here standard values No CAL appears in the display CAL 1000 O ENTER Forchanges see Sec 5 11 1 Select Smaller volume for Filtertips nen O ENTER here changing from 10 ul to gt 8 ul for Filtertips 300 ul pipette with 200 pl tip restriction of 200 pl After changes have been made down to the smaller volume a reminder to make the necessary changes for volume programming is also issued by means of the error message VOL 2 To exit the device parameters INP O ENTER For information on the INI procedure see 5 11 2 5 11 1 Changing the calibration CAL The Research pro is ideal for pipetting solutions with a density viscosity surface tension and vapor pressure which are not considerably different to that of water It is not necessary to alter the calibration when such solutions are used If due to the physical properties of the solution a pipetting volume is produced which is different to the volume displayed
24. e tip is attached Do not lay down the Research pro when a filled pipette tip is attached After liquid has been aspirated press the Actuate key only when you are sure that the liquid shall be dispensed correctly When the pipette is switched on and when the Reset key is pressed the piston may move Please ensure beforehand that the liquid in the pipette tip shall be dispensed correctly Emergency stop A moving piston can be stopped by pressing the Reset key When using infectious radioactive toxic and other solutions which may pose health risks please observe the safety precautions laid down for your country Do not use the Research pro in a potentially explosive environment or with potentially explosive chemicals When using organic solvents and aggressive chemicals please check their compatibility with the pipette tip PP and pipette When using solutions with physical properties which deviate greatly from those of water carry out the procedure for checking the dispensing volume as described in Sec 5 11 1 When using multi channel models of the Research pro rotate the multi channel adapter in the permitted direction of the arrow only see Sec 6 Maintenance Operate the Research pro at temperatures between 15 C and 40 C only and at a humidity of max 80 at a temperature up to 31 C When using Filtertips or 200 ul tips with the 300 ul pipette activate the volume restriction option see Sec 5 11
25. ecla hasta que aparezca EDIT Select SP Seleccionar SP Selection of the pipetting sequences After ENTER Select 1 for the first sequence in the display Selecci n de las secuencias de pipeteo Tras ENTER Seleccionar 1 para la primera secuencia en el visualizador Select the first volume and store Seleccionar el ler volumen y memorizar Select the speeds Seleccionar velocidades Select the option Seleccionar la opci n Select the second volume Seleccionar el 2 volumen Programming is complete EDIT disappears La programaci n est completa EDIT desaparece 88 apod Programs 89 Part B Programs Parte B Programas Procedure Ejecuci n _ Sample is aspirated If O 5P tae pl appears in the display press Se absorbe la muestra Si aparece en el visualizador pulsar D How you now proceed depends on the pipetting option selected and on the number of sequences The procedure carried out according to the option is identical to that described in Sec 5 3 to Sec 5 7 El procedimiento siguiente depende de la opci n de pipeteo seleccionada y del n mero de secuencias La ejecuci n seg n la opci n es id ntico al descrito en el cap 5 3 al cap 5 7 Ill DIS Dispensing as a program DIS Dispensar como programa As a program dispensing is protected from any accidental alterations Please observe the volume restriction for Filtertips see Sec 5 11
26. ed A special acoustic signal is emitted Blow out is not executed Blow out can be actuated by pressing the amp key Press the key again to aspirate liquid The direction can also be changed during dispensing The volume display changes after the Actuate key has been pressed 5 Operation 5 8 Pipetting with a preselected fixed volume FIX FIX is used to rapidly set and recall five frequently used pipetting volumes Five FIX volumes are predefined in the pipette FIX volumes can be changed via programming Programming FIX fixed volumes Select Sr Program Co ii ul on o o o 2 el Ti EINE gt lt 3 o 5 Ti T A gt lt ES D Program p FIX 300w 3 or uo If PROG appears in the display Press the eo key briefly Hold down the Program key until EDIT appears The volume flashes Select the memory slot using the Select rocker Using the Volume rocker select the volume for the memory slot here 5 Select the next memory slot for purposes of changing the volume Define the next volume here memory slot 3 The program is ended by pressing the S key EDIT and the flashing volume disappear from the display The selected volume is aspirated Programming is ended EDIT and the flashing volume disappear from the display If appears in the display Press the key briefly 16 Operation 17 5 Operation Selecting a fixed volume
27. ed by aspirating air bubbles The display shows the total volume uo 2 Dispensing Titration is carried out The amount of liquid dispensed appears in the display There is no blow out Setting lower speeds is strongly recommended Programming Select y If PROG appears in the display Program A Prone Press the key briefly LOE Volume Volume selection here 1 000 ul The volume rocker has two pressure points for up and down Option Option ation 7 lt q If necessary press the Option De PIP 1008 key several times MAN is za selected Carrying out liquid aspiration Liquid is aspirated The aspirated liquid appears in the display If appears in the display and no volume is shown Press the D key briefly The direction is changed using the volume rocker eal ia Se Ea EG 4 e g for removing an air bubble Volume correction Continued on next page 14 Operation O O 00 O Note Volume y 15 The direction is changed again using the volume rocker This change of direction can be repeated without restriction A defined end volume has been reached A special acoustic signal is emitted Dispensing is carried out by pressing the key again Aspirate the defined total volume by holding down the Actuate key When the key is pressed briefly The amount of liquid dispensed appears in the display Total amount of liquid is dispens
28. ejector sleeve 1 Force may be required Unscrew the lower part 2 using the key 3 During disassembly do not damage the piston Unscrew the piston 4 If necessary first loosen the piston at the upper end using the flat nose pliers The piston is moved into ts lowest position For 5 000 pl A 4 2 1 Hold down the ejector and pull off the ejector sleeve 1 Force may be required U nscrew the cylinder 2 The piston 3 is held in place magnetically During disassembly do not damage the piston Pull the piston 3 out of the upper part Using the disassembly tool in the accessories package 4 unscrew the cylinder bearing During removal the spigots of the disassembly tool are inserted into the openings on the cylinder bearing Assemble in reverse order Engage the magnetic coupling of the 5 000 ul piston as shown in the diagram Check that the dispensing function of the pipette is working correctly 6 3 Maintenance Apart from general care and occasional discharging when the battery symbol starts flashing and recharging of the pipette no special maintenance is necessary if the pipette is used correctly The lower part of the pipette may be replaced completely How to disassemble the pipette is described in Sec 6 2 The process for replacing the seals in the lower part is described in Part B Ordering information service parts Maintenance on the Research pro may be carried
29. en the pressure piece using the punch B on the tool The pressure piece is under spring tension Hold the piece using your forefinger Pull out the springs and the sealing ring using the tool 4a Reassemble in reverse order The parts are placed onto the tool 4b Move piston in upper position After assembling hold down the Reset key in order to trigger the motor reset and check that the device is functioning correctly 1 Con el destornillador apretar en la muesca y sacar el cuerpo 2 Con la herramienta apalancar las pinzas met licas del gancho de seguridad Esta pieza se encuentra bajo tensi n el stica Sujetar la pieza como se muestra en el diagrama y aflojar la pinza met lica por el otro lado 3 Sacar el portaboquillas conos de trabajo Precauci n El equipamiento irregular de los resortes de los pistones es deliberado Anotar el equipamiento de resortes y observar durante el montaje Antes de limpiar el pist n con 60 isopropanol sacar los resortes Lubricar ligeramente el pist n con grasa de silicona 4 Aflojar el pisador con el mandril B en la herramienta El pisador se encuentra bajo tensi n el stica Sujetar la pieza con los dedos ndice Sacar los resortes y el anillo obturador con la herramienta 4a Remontaje en sentido inverso Las piezas se colocan en la herramienta 4b Llevar el pist n a la posici n superior Despu s del montaje pulsar largo la tecla Reset para provocar un reset de motor y controlar e
30. evel by using the Select rocker or by using the Option or Speed key Descriptions of how to use the Option and Speed keys can be found in Secs 5 2 5 9 We recommend that you read these sections before you start programming The following section on programming for pipetting contains only a description of operation using the rockers Programming and execution of the other programs is contained in Part B of this manual which is printed in English and Spanish 20 21 5 10 1 Example for programming pipetting in the program level Programming Program a D e Select eS 5 Program gt PROG 12345 Select Ga s PROG 12345 or Volume Select PI IP PROG 12775 Select PN 400 ol Eni proc 12348 Select a PIP sg PROG 1235 A PROG 12345 Press the key briefly until PROG appears in the display Select the memory slot Hold down the key until EDIT appears The input field flashes Select PIP Press the Actuate key The input field flashes Select the volume and store Select the aspirating and dispensing speed and store The direction arrow flashes The option is selected and stored The magnifying glass shows the standard mode Programming is now complete here standard mode no option EDIT disappears Programming can be ended prematurely and then stored by pressing the eo key EDIT disappears from the display If EDIT is ended with
31. ew enables the hook on the rear of the pipette to be adjusted to suit all hand sizes This can be done easily with a small Phillips screwdriver Contact surface on the rear of the pipette Both upper surfaces are required for charging the nickel metal hydride battery The lower surfaces are the data interfaces for the Service Department The contact to the charging adapter or the charging stand cannot be confused with any other contacts Acoustic signals Certain signals assist the user for different operations Short signal to acknowledge that the keypad has been pressed Slightly higher tone to indicate that the function for which the key has been pressed cannot be executed Louder tone to indicate that a piston movement has ended Longer tone to indicate that a specific sequence of steps e g Dispensing has ended Louder tone to indicate an error message The acoustic signals are a great help when you are familiarizing yourself with the operating procedure of the Research pro They can also be switched off if required see Sec 5 11 5 Operation 5 1 Mode of operation If the display of the Research pro is dark the pipette is switched on by pressing any key firmly If the pipette has not been used for a long period a motor reset is executed after it has been switched on Attach an Eppendorf pipette tip to the Research pro The color coding of the Actuate key corresponds to the color code of the racks for pipette tips
32. iiiiii iiic ini sen nene nenet Cambio unidad de volumen 50 1200 Ul cece tr tee inet retn cnn iii rr Ennn 0 unn nens Avisos importantes a las informaciones para pedido Cambio piezas de servicio multicanal 86 SONY E ed MPO E ed Programs 87 Part B Programs Parte B Programas I Introduction Introducci n This section contains a complete description of the additional six program sequences see Sec 5 10 During programming EDIT and PROG in display the Speed and Option keys and the Volume rocker can also be used This enables users to skip backwards and forwards during the program sequence When the programs are executed only PROG is in the display the Speed and Option keys and the Volume rocker are blocked If only parts of the program have to be changed programming can be ended prematurely by pressing the Program key briefly If a memory slot is quit using Reset before programming has been completed the changes which have been carried out for this memory slot are not carried out EDIT disappears from the display Esta parte contiene una descripci n completa de los otros 6 desarrollos de programa adicionales ver cap 5 10 Durante la programaci n EDIT y PROG en el visualizador pueden utilizarse tambi n las teclas Speed y Option y el selector de volumen Esto permite a los usuarios saltar hacia delante y atr s durante la secuencia de programa Al ejecutar lo
33. illing of the pipette tip here 20 1 000 ul pipette If necessary the number of dispensing steps can be minimized The dispensing steps are flashing during Select Display and selection of aspirating speed Display and selection of dispensing speed Aspiration of total amount of liquid here gt 600 ul If appears in the display Press the key briefly First dispensing step is executed Second dispensing step is executed 18 on 19 v 4 Last dispensing step is ms 50 executed Here special acoustic signal is emitted Blow out is discarded q 3 No dispensing step If the Actuate key is held down during the final blow out the device does not return a to the basic position until the key is released Blank step with special acoustic O WS Soe signal y Pipette is once again ready to 5 59e P aspirate liquid 5 10 Notes on the programs Program To reach the programming level the _ key must be pressed briefly PROG appears in the display together with a memory slot number from 1 5 Select With the aid of the rocker five programs can be shown in the left hand side of the display After programming has been completed the procedure can no longer be changed by repeatedly pressing the keys or the volume rocker To program the five memory slots it is possible to select from seven different program sequences PIP Pipetting As per PIP outside the progra
34. irst time 3 3 Charging the battery The battery is charged in the pipette Charging may only be carried out using the charging adapter supplied orthe charging stand with the original power unit Do not try to recharge a pipette if the battery is not installed A new battery does not attain its full capacity until two or three complete charging discharging cycles have been completed Before recharging please compare your voltage requirements with the voltage specifications on the plug in unit For charging purposes the charging adapter is placed upon the Research pro Alternatively the Research pro can be placed in the charging stand During the start and termination of the charging process CON appears briefly on the left hand side of the display The display is switched on during the charging process The battery symbol appears in a rolling form During charging the Dispensing function is frozen When the charging process has finished the battery symbol appears in the display without moving When the pipette has been fully charged it may remain in the charging stand To increase the charging capacity the battery should occasionally be discharged until the flashing battery symbol appears To avoid a total discharge the battery should be removed from the Research pro during extended periods of non use e g several months In this case all data remains stored 4 Operating principle 4 1 Display and keypad Volume and unit
35. l charging unit con adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga Eppendorf Research pro 8 channel 8 canales incl charging unit con adaptador de carga incl charging unit con adaptador de carga incl charging unit con adaptador de carga incl charging unit con adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga o o Eppendorf Research pro 12 channel 12 canales incl charging unit con adaptador de carga incl charging unit con adaptador de carga incl charging unit con adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga without charging unit sin adaptador de carga Pipettes sold without a charging unit must be charged in the optional charging stand Pipetas vendidas sin adaptador de carga tienen que cargarse con el cargador opci nal Charging stand Cargador for one pipette para una pipeta for four pipettes para cuatro pipetas 05 10u 5 1004 20 300 u 50 1000 u 100 5000 ul 05 10u 5 100 pl 20 300 u 50 1000 u 100 5000
36. l funcionamiento correcto del dispositivo r C TA Door s cr A EG Konformitatserklarung EC Conformity Declaration Eppendorf Netheler Hinz GmbH Barkhausenweg 1 22339 Hamburg Germany Dias tmpeici nato Ger t entapricht den sinectiagigen yundegeden Anioedenmngen der agihan DG Tchtieken und Nornen Bei cheer font mit une agentes Ancenung clas Gardies werhert devo Erkl rung hre Girighet The devine named belos Aide the rievani fundamental recumrenments of me EC chectives and stancerce Exted In Pe case of unauthorised Mods 10 Ma device thes dectaration becomes rabo Tolerance Check Protocole de mesure Protocollo de medic n eppendorf In touch with life B 4860 900 020 00 1299 Printed in Germany Printed on chlorine free bleached paper eppendorf is a registered trademark Brinkmann Instruments Inc One Cantiague Road P O Box 1019 Westbury New York 11590 0207 USA Phone 800 645 3050 Fax 516 334 7506 e mail info brinkmann com Brinkmann Instruments Ltd 6670 Campobello Road Mississauga Ont L5N 2L8 Canada Phone 800 263 8715 Fax 905 826 5424 e mail brinkmann on aibn com
37. m level The complete sequence with volume option and speeds is programmed in one memory slot SP Sequential Pipetting Up to five different pipettings can be linked up to each other Volume option and speeds are defined for each pipetting sequence DIS Dispensing As per DIS outside the program level The complete procedure is programmed in one memory slot with dispensing volume dispensing steps and speeds 5 Operation SD Sequential Dispensing Up to 20 dispensing steps can be defined Each dispensing step may have a different partial volume The total volume may exceed the filling level of a pipette tip This means that new liquid for the pipette tip must be aspirated during the dispensing procedure Only one aspirating and dispensing speed is defined for all dispensing steps ADS Automatic Dispensing With the Actuate key held down all dispensings are executed automatically with the same volume and in a user defined rhythm 0 1 10 0 seconds Automatic dispensing may be interrupted by releasing the Actuate key Apart from this the procedure is identical to the DIS function DIL Diluting During the diluting process a diluent then an air bubble and finally a sample are aspirated into the pipette tip The diluent volume and the sample volume are defined by the user The air bubble is dependent on the sample volume and is always specified by the program The entire contents of the pipette tip can be dispensed by selecting a
38. ntaje ver cap 6 3 5 100 ul and y 20 300 pl 50 1200 yl 8 pieces 8 piezas 2245 635 1 2246 713 1 Lower parts cpl 8 channel Piezas inferiores cpl 8 canales 0 5 10ul 2246 714 0 5 100 ul 2246 715 8 20 300 ul 2246 716 6 50 1200 ul 2246 717 4 Lower parts cpl 12 channel Piezas inferiores cpl 12 canales 0 5 10ul 2246 718 2 5 100 pl 2246 719 1 20 300 pl 2246 720 4 Important notes regarding ordering information Piston seals for multi channel model Set of piston seals Change service parts see below and next page Juntas de pist n para multicanal Set de juntas de pist n Cambio piezas de servicio ver abajo y p g siguiente 0 5 10yul 2245 653 9 5 100 ul 2246 721 2 20 300 ul 2245 655 5 Volume unit Unidad de volumen 50 1200 ul 2246 722 1 Change volume unit 50 1200 pl Loosen the srews in the housing lower part and lift the top half of the lower part Press down the volume unit below which is to be exchanged so that the upper part is lifted out of the groove Cambio unidad de volumen 50 1200 pl Aflojar los tornillos de la semicarcasa inferior y levantar la mitad superior de sta Bascular la unidad de volumen a cambiar apret ndola abajo de modo que la parte superior salga fuera de la ranura Only parts with order numbers are available Please only use the accessories recommended by Eppendorf Using disposables which we have not recommended can reduce the imprecision inaccura
39. ntas en la pieza inferior V ase p g siguiente Cambio piezas de servicio v ase p g siguiente 0 5 10 Ul 2247 665 3 3 Sealing set 5 100 yl 2247 669 6 3 juego de juntas 20 300 ul 2246 710 7 5 100pl 2247 528 2 50 1000 ul 2247 672 6 20 300ul 2246 712 3 100 5000 pl 2246 711 5 50 1000 ul 2247 646 7 Cylinder and cylinder attachment for100 5000 pl Cilindro y toma del cilindro para 100 5000 ul 5 Reducing tube 5 pieces 1 wire punch 5 Tubo de carga 5 piezas 1 mandril expulsor 5 1004 20 300 yl 2247 655 6 2247 656 4 102 oppad erd SSUODELUIOJUI UORPLULIO JU G6ULBRIO ing information Informaciones para pedido 103 Part B Ordering information Parte B Informaciones para pedido Change Service parts single channel Cambio piezas de servicio monocanal 0 1 0 0010 Ir 5 100 pl 20 300 pl 50 1000 pl The seals in the lower part 1 are unscrewed from the lower part which has been separated from the pipette see Sec 6 2 using the wrench 2 The lower parts for 0 5 10 ul and 100 5 000 ul do not contain any seals With the 10 100 ul pipette side B of the wrench 2 is used for screwing unscrewing Parts of the seal are then pulled out using side C 3 With the other pipettes the seals are unscrewed using side C of the wrench or are tapped out after having been loosened For mounting the parts are placed onto the wrench 4 When screwing t
40. ogether do not tighten too much The filling tube 5 is delivered as a spare parts pack together with a wire punch After assembling hold down the Reset key in order to trigger the motor reset and check that the device is functioning correctly Las juntas en la parte inferior 1 se desenroscan de la parte inferior de la que se han separado de la pipeta ver cap 6 2 con la llave 2 Las partes inferiores para vol menes 0 5 10 uly 100 5 000 ul no llevan juntas Con la pipeta de 10 100 ul se usa el lado B de la llave 2 para enroscar desenroscar Despu s se sacan las partes de la junta con el lado C 3 Con otras pipetas las juntas se desenroscan con el lado C de la llave o golpeando hacia afuera despu s de haber aflojado Para el montaje se colocan las partes en la llave 4 Al enroscar no apretar demasiado El tubo de carga 5 se entrega como paquete de pieza de repuesto equipado con un mandril expulsor Despu s del montaje pulsar largo la tecla Reset para provocar un reset de motor y controlar el funcionamiento correcto del dispositivo Part B Ordering information Parte B Informaciones para pedido Spare parts for multi channel model Piezas de repuesto para modelo multicanal The disassembly procedure can be found in Section 6 3 Para el procedimiento de desmontaje leer el cap 6 3 O rings for nose cone 12 pieces incl mounting aid see Sec 6 3 J untas t ricas para conos de trabajo 12 piezas con ayuda de mo
41. on discard the pipette tip after that by pressing the Eject key attach a new tip and reaspirate liquid y If necessary carefully remove any external wetting from the pipette tip 5 2 Essential operating information The following sections contain step by step explanations of the operating procedure It is essential to work through these sections with the pipette in your hand The volume shown in the display information contained in the operating manual does not necessarily correspond to the volume range of your pipette Reset Ifa Reset is requested in the display this Reset is always accompanied by a piston movement For this reason please ensure that any residual liquid in the pipette tip is d dispensed beforehand A Reset can also be used to empty the pipette tip and to end an operating process If you quit an input field during programming e g changing the speed using Reset the changes which have been made are not stored The Reset button can also act as an emergency stop function on 5 Operation Motor If the Reset key R is held down until the piston movement begins and R appears in reset the display a motor reset is carried out This motor reset guarantees that the piston movement is correct This reset routine lasts for approximately 10 seconds Program The Research pro has two operating levels The basic modes for pipetting PIP and lt lt FIX and dispensing DIS are accessed in the first level by pressing the
42. out by the Service Department Contact Brinkmann Instruments at 800 645 3050 Ext 404 In the case of the lower parts of the eight and twelve channel models it may be necessary to replace the O rings seals for pipette tips which means that the lower part must be unscrewed A tool for replacing the O rings is contained in the accessories package Press opening A of the tool over the nose cone The A cee 2 sharp edge in the opening of the tool fits together with T the O ring oe To cut the O ring press the tool heavily against the nose cone The tool and the O ring are then removed Put the assembly aid a shortened pipette tip onto the nose cone and slide the new O ring onto the nose cone 7 Troubleshooting d Display information Cause Solution v NL Middle segment The battery is run down Complete pipetting and a flashes recharge the battery see IN Sec 3 3 NSi All segments flash The battery is severely run down Stop pipetting immediately 1 and recharge the battery see Sec 3 3 4 Segments roll The charging process is Charge the pipette until the SunooyssmmoiL I ough the display underway battery symbol appears in the N A All dispensing functions are display without moving blocked 74 ar ments appear Charging process is finished Pipette is ready to use again In the alspla without mound The pipette does not cont
43. pears in the display Hold down the Program key until the number flashes Set the desired number using the Volume or Select key The CAL symbol appears when b 0 0 Confirm the number selected by pressing ENTER The entry for m flashes This can also be changed by pressing Volume or Select Entries are stored by pressing ENTER The ranges for m and b differ slightly according to the volume size of the pipette Note Following programming the calculated data must then be checked via weighing and by using formula 1 Hint In the case of mechanical pipettes only axis section b can be corrected 24 on 25 5 Operation 5 11 2 Information on initialization INI If the Research pro is to be used at another workstation the INI function returns the pipette to the original factory calibration If all volumes and programs defined by the user should no longer be used the pipette can be initialized as per delivery package by means of INI Following initialization data previously programmed by the user can no longer be activated Initialization is carried out as follows Using ENTER access INI in the device parameters Hold down the Program key Initialization is completed when the device parameter INI disappears from the display 6 Care sterilization and maintenance 6 1 Care The outside of the pipette can be wiped with a moist cloth The use of water with wetting agent
44. petting Il DIS Dispensing AS a program zoms dah rr 89 IV SD Sequential DISPENSING r userssennneneennennnennnnnnnnennnnnnnnnnnnnnnnnnnsnnnnnnnnnnnnsnnnnnnnnn nenn 90 V ADS Automatic Dispensing VI DIL Biluingan aaa en Maniatis MIS DT Senat DMN Gi anat ira Ordering information cei eee ee niin nine rr 100 Research PrO erso iooi ak 100 Pipette COS torio nannte 101 Additional accessories 102 Spare parts for single channel pipettes 102 Change Service parts single channe eee 103 Spare parts for multi channel model es 104 Change volume unit 50 1200 Ul ee iasi 104 Important notes regarding ordering information RR 104 Change Service parts multi channel cece eee te ieee cnn rin 105 Programas l Introducci n r ll SP A 6590960065 87 Il DIS Dispensar como programa suasccasinnan du N rr 89 IV SD Dispensaci n secuencial V ADS Dispensaci n autom tica Mi DIL DilUiticwntacic chen sen a Aine ans PNA eek Lande VIL SD DIlUir en SONG i sctcs settee icine kenn Informaci n para pedidoS cummins eerste d rr rr 100 Research Dl ech as Boquillas para pipeta in Otros ACCESOS EAS Piezas de repuesto para pipetas MONOCANA ueerssernsennnnensennnnnnnnnnnnnennnnnnnnnnnnn rar 102 Cambio piezas de servicio monocanal sis ta Piezas de repuesto para pipetas multicanal iiiiiiiiiiiiiiiiiiiiii
45. pipetting option Depending on the pipette used the dilution ratio is between approximately 1 2 and approximately 1 20 SDI Serial Diluting For serial diluting a defined volume of liquid is aspirated into the pipette tip This liquid is dispensed and then mixed with a specific liquid volume by means of a programmable mixing process volume mixing cycles The mixing procedure MIX is started by pressing the Actuate key separately EDIT If PROG is in the display the programming level can be opened by holding down the Program key The Program key must be held down until the word EDIT appears in the display in front of PROG 1 5 Step by step programming is explained in the following sub section If the programming of a program sequence is quit by pressing RESET the entries for the program sequence are not stored uo The same program sequence with for examp in several memory slots It would therefore be p stored permanently in the program level Program sequences can be overwritten at any ti e different volumes or options can be stored ossible for different pipetting sequences to be ime under EDIT Hold down the Program key During the programming process the entry which is to be defined flashes in the display Selections are made using the Select rocker Volumes are selected using the Volume rocker Data is stored by pressing the Actuate key ENTER function Options and speeds can be selected in the programming l
46. ps the procedure for normal dispensing is applicable when a liquid is aspirated repeatedly Please observe the volume restriction for Filtertips see Sec 5 11 Este programa puede usarse cuando se tienen que dispensar de forma consecutiva diferentes vol menes de una soluci n Pueden definirse hasta 20 pasos de dispensaci n Si la suma de los vol menes de los pasos de dispensaci n es mayor que el volumen de las boquillas de pipeta se aplica para la absorci n repetida de un l quido el mismo procedimiento como para una dispensaci n normal Por favor observar los l mites de volumen para Filtertips ver cap 5 11 Programming Programaci n Program p PROG in the display N O e PROG en el visualizador Select Select the memory slot ES Seleccionar el puesto de memoria 90 apod Programs 91 Part B Programs Parte B Programas Program Qo Select Select or o Volume Select or o Volume Select Il Select Il amp PROG 12345 51 PROG 12345 5 SEH ET proc 17 Em proa 17 5 De co Eat Proc 12345 ej oj o ele ENTER ENTER ENTER ENTER ENTER Hold down the key until EDIT appears Pulsar largo la tecla hasta que aparezca EDIT Select SD Seleccionar SD Determine the dispensing steps 1 20 Determinar los pasos de dispensaci n 1 20 Volume of the first dispensing step Volumen ler paso de
47. right of the display If the device is reset to the original values the CAL symbol disappears If the CAL symbol appears in the display the stroke movement is converted by the pipette using m and or b which is selected by the user in order to ensure that the actual volume of the liquid used corresponds to the display volume uo It is essential to inform all users of the changes made to the calibration data We recommend labeling the pipette as follows example only Pipette calibrated only for use of xy with option ZZZ Examples for volume correction 1 An actual volume of 98 ul was calculated using formula 1 Balance in mg density in mg ul g ml The display volume is 100 ul In this case the following formula should be used Display volume 100 ul 2102 Actual volume 98 pl The value 1 02 is entered under m This means that 100 ul appears in the display although the piston makes a stroke movement which is larger by a factor of 1 02 so that 100 ul of this liquid can be aspirated 2 For corrections across a large volume range the actual volumes for at least two greatly differing volumes should be determined Via linear regression calculator y mx b see formula 2 the data for m and b is calculated by entering the actual volume x and the display volume y The calibration data can be changed as follows Call up the device parameters see 5 11 Proceed using ENTER until CAL 0 0 UL b ap
48. s or downwards Pressed briefly Individual changes Held down Rapid changes 2nd pressure point High speed Automatic stop for min max volume and for overflow obtained by releasing and then pressing the rocker again needo puud Su ad h ng principle 4 Operating principle 4 2 Unit s functions The Research pro is a microprocessor controlled pipette which executes the piston movement with the aid of a stepper motor The power supply is a rechargeable nickel metal hydride battery Upper end position gt eee Basic position I End of blow out For sample aspiration the Return to the basic position Residual liquid piston moves upwards from via short ssinale st discarded via the basic position ne ort single steps blow out BLOW The sample is aspirated into u Bpensing FOREN the pipette tip entire path Pipetting Dispensing and Reverse Pipetting require a different basic position than that for Pipetting The request to change the basic position is indicated in the display by Ejector with extension NS The force translation of the ejector for pipette tips ensures that tips are ejected A A very easily The extension can be individually adjusted to accommodate left and right handed users as well as different hand sizes Select the desired setting by simply sliding the extension into the position as required Ejector Extension Hook on the hand rest Loosening the scr
49. s programas s lo PROG en el visualizador las teclas Speed y Option y el selector de volumen est n bloqueadas Si se deben cambiar solamente partes del programa la programaci n puede finalizarse prematuramente pulsando brevemente la tecla Programa Si se sale de un puesto de memoria con Reset antes de haber finalizado la programaci n no se asumen los cambios realizados para este puesto de memoria EDIT desaparece del visualiza dor Il SP Sequential Pipetting Pipeteo secuencial This program is suitable for sequences in which different volumes have to be pipetted consecutively on a regular basis It is possible to program up to five volumes with option and speed Este programa es adecuado para secuencias en las que deben pipetearse de forma consecutiva diferentes vol menes regularmente Pueden programarse hasta 5 vol menes con opci n y velocidad Programming Programaci n Program ae PROG in the display PROG en el visualizador Part B Programs Parte B Programas Program Select Select Select or o Volume Select Select Select Proc O PROG 12345 EDIT PROG 12345 Sen ERIT proc 12778 IE 20 PROG 1 O ENTER ENTER O ENTER ENTER ENTER OO O Select the memory slot Selecci n del puesto de memoria Hold down the key until EDIT appears Pulsar largo la t
50. ssion The automatic dispensing function allows a single volume to be dispensed repetitively with a predetermined time interval of between 0 and 10 seconds This program is recommended for such an application Please observe the volume restriction for Filtertips see Sec 5 11 Al llenar placas de microtitraje tiene que dispensarse una cantidad espec fica de l quido en sucesi n r pida La funci n de dispensaci n autom tica permite dispensar un volumen sencillo repetidamente con un intervalo predeterminado de tiempo entre 0 y 10 segundos Este programa se recomienda para esta aplicaci n Por favor observar los l mites de volumen para Filtertips ver cap 5 11 Programming Programaci n 7 PROG 12345 Select A PROG in the display PROG en el visualizador Program n Qo Select the memory slot Seleccionar el puesto de memoria Il Program Hold down the key until EDIT cx a DIS Pulsar largo la tecla hasta que aparezca EDIT Select Select ADS a ENTER Seleccionar ADS or o Select the dispensing volume Select Volume AIS ay ENTER and store Eei Seleccionar el volumen de rw dispensaci n y memorizar Select Select the aspirating and O ENTER dispensing speed L Hise Seleccionar la velocidad de absorci n y de dosificaci n Select 5 Reduction of the dispensing ADS O O ENTER steps EDT PROG 12345 A Reducci
51. t ndar en bolsa 1 000 unid 254 2235 142 7 20 u 2235 156 7 200 u 2235 130 3 300 u 2235 141 9 1000 p 2235 090 1 1 25 m 2226 570 9 5 ml 500 unid 2235 081 1 Enviroset 1 Envirobox autoclavable plus 7 x 96 Envirotips in racks Enviroset 1 Envirobox autoclavable m s 7 x 96 Envirotips en racks 2 5 ul 2249 049 4 20 ul 2249 050 8 200 ul 2249 055 9 300 ul 2249 057 5 1000 ul 2249 060 5 1 25 ml 2249 065 6 Envirotips tip refills 10 x 96 960 tips Envirotips en racks 10 x 96 960 unid 2 5 ul 2249 039 7 20 ul 2249 040 1 200 ul 2249 042 7 300 ul 2249 043 5 1000 ul 2249 044 3 1 25 ml 2249 045 1 Enviroboxes 1 Envirobox incl 96 tips Enviroboxes 1 Envirobox plus 96 unid 2 54 2249 079 6 20 u 2249 080 0 200 u 2249 085 1 300 u 2249 087 7 1000 u 2249 090 7 1 25 m 2249 095 8 5 ml 28 tips unid 2249 105 9 Eppendorf Biopur pipette tips colorless sterile pyrogen free DNA free RNase free ATP free in boxes 5 x 96 480 tips Boquillas de pipeta Eppendorf Biopur incoloras est riles sin pir geno sin DNA sin RNasa sin ATP en cajas 5 x 96 480 unid 20 ul 2249 000 1 200 ul 2249 002 8 300 ul 2249 003 6 1000 ul 2249 006 1 Eppendorf Biopur pipette tips individually wrapped 1 set 100 tips Boquillas de pipetas Eppendorf Biopur envase individual 1 juego 100 unid 200 ul 2249 004 4 1000 ul 2249 008 7 Filtertips sterile in boxes 5 x 96 480 tips Filtertips est riles en cajas 5 x
52. t of multi channel model To loosen the lower part of the multi channel pipette rotate Cl 0 x 1 it in the arrow direction which is normally not permitted Ne A Pull the lower part downwards slowly The multi channel Opening for piston which is held in place magnetically jerks sharply and metal pin then comes off Piston When reassembling please observe the following Hold down the ejector for pipette tips see Sec 4 1 Position the lower part in such a way as to enable the metal pin on the ejector to enter the corresponding opening on the lower part The magnetic coupling of the piston of the upper and lower part is indicated by a clicking noise Do nothold down the ejector any longer and push the lower part firmly onto the metal pin of the ejector Rotate the lower part in the permitted direction When the lower part is rotated a low click indicates that the safety coupling has connected and the lower part is firmly in place After assembling the pipette check that the ejector is working correctly After assembling the pipette actuate the motor reset by holding down the Reset key Check that the dispensing function of the pipette is working correctly 26 SOUBUBIUIELU pue UORPZILSE Qar Care sterilization and 27 6 Care sterilization and maintenance Lower part of single channel model All volumes except 5 000 pl 4 Hold down the ejector and pull off the
53. the E key before completion of programming for the memory slot the changes are not transferred emergency exit 5 Operation Procedure The sample is aspirated O PIP Sog PROG 12345 The remaining procedure depends on the pipetting option selected Depending on the option the procedure is identical to Sections 5 3 to 5 7 uo When Prog 1 5 are carried out the Option and Speed keys as well as the Volume rocker are all disabled 5 11 Device parameters Device parameters may be changed only after thorough inspection and only by persons who are trained to do so Device parameters are made up of the following BE 1 To switch on off the acoustic signal for the keypad short beep BE 2 To switch on off the second acoustic signal for the end position of the dispensing piston for warning and for confirmation different beeps CAL To alter the calibration data ul axle section b and gradient m OPT Option for volume restriction When the 200 ul tips are used with 300 ul pipettes limit 200 ul With RP and DIS when Filtertips are used Volume restriction ensures that no liquid comes into contact with the filter during RP and DIS It also guarantees that no liquid enters the pipette when 200 ul tips are used INI To initialize the original pipette data upon delivery The device parameters can all be found on one list The Actuate key functions as an Enter key After Enter has been pressed th
54. u 0 5 10u 5 100u 20 300 u 50 1200 u 0 5 10u 5 100 pl 20 300 u 50 1200 u 05 10 5 100u 20 300 u 05 10yu 5 100 pl 20 300 u If the delivery address is not in the country in which the device shall be used When placing your order please specify the country in which the device is to be used and the voltage used in that country Only then is it possible for us to deliver the power unit of the charging unit stand with the necessary input voltage Si la direcci n de entrega no se encuentra en el pa s destino 2246 130 3 2246 131 1 2246 132 0 2246 133 8 2246 134 6 2246 135 4 2246 136 2 2246 137 1 2246 138 9 2246 139 7 2246 140 1 2246 141 9 2246 142 7 2246 143 5 2246 144 3 2246 145 1 2246 146 0 2246 147 8 2246 148 6 2246 149 4 2246 150 8 2246 151 6 2246 152 4 2246 153 2 2246 154 1 2246 155 9 Por favor indicar en el pedido el pa s destino especificando la tensi n all usada S lo as podemos entregar la fuente de alimentaci n del adaptador de carga cargador con la tensi n de entrada necesaria 100 oppad erd sSU0DEULIOJU UO0RULIOJU BULSPUO ing information Informaciones para pedido 101 Part B Ordering information Parte B Informaciones para pedido Pipette tips Boquillas de pipeta The packing units stated represent the minimum order quantity Las cantidades indicadas representan la cantidad m nima de pedido Bulk packaged tips in bags 1 000 tips Boquillas es
55. ume ranges The pipette is delivered with or without a charging adapter depending on the order number If the pipette is ordered without an adapter a charging stand for one or four pipettes is required If this charging stand is not already available in the lab it must be ordered separately According to the type of order the delivery package contains the following Charging adapter with connected power unit or if necessary charging stand with separate power unit Special tool s according to the pipette type Operating manual with measuring protocol and CE conformity assurance document Ni MH battery pack For multi channel pipettes Reagent reservoir for liquid aspiration e o o o 3 2 Inserting the battery N Using your thumb and forefinger take hold of the lid of the battery popuadda compartment on the rear of the pipette and remove it by tilting it to the side a i and pulling it upwards Insert the battery pack see diagram Close the lid of the battery compartment If the battery has been inserted sw incorrectly the lid cannot be closed properly The positive and negative poles of the battery should not come into contact with any metallic objects outside the pipette When the battery is inserted the Research pro carries Program Speed Option ZZ 23 25 outa test routine This takes approximately 15 20 seconds The battery must be fully charged before the device is used for the f
56. with option and speed Select p If PROG appears in the display Program f Press the 222 key briefly Volume Select from the five FIX volumes FISO Press the Option key repeatedly 300 until the desired option appears Rum BLO RNS RP MAN or nothing Speed Select Display and selection of FI eo aspirating speed Option Option tion r COD FI Display and selection of dispensing speed Speed Select Speed gt H Note The option and speeds selected are applicable for all fixed volumes It is possible to store the pipetting volume with speed and option by using Prog Sec 5 10 Procedure The procedure depends on the option selected Information on the procedure according to the option selected is contained in Sections 5 3 to 5 7 5 9 Dispensing DIS During this procedure the aspirated liquid is dispensed in defined partial steps As with Reverse Pipetting slightly more liquid is aspirated than is necessary for the sum of the dispensing steps When Filtertips are used please observe the volume restriction Sec 5 11 Programming Select Volume E E Option Select Speed Select ED Speed Select a gt Procedure O Option Speed gt s E 17 A y Es FS AKD Es FS uone pdo If PROG appears in the display Press the oe key briefly Select the volume per dispensing step In the right hand side max dispensing steps per f
57. y dispensaci n iSeleccionar una velocidad alta Stroke for mixing the specimen liquid and the sample Carrera para mezclar el l quido ya preparado y la muestra Mixing cycles max 20 Ciclos de mezcla m x 20 BLO pipetting option or standard operation Opci n de pipeteo BLO o modo est ndar Aspiration of sample If appears in the display press the key Absorci n de muestra Si aparece en el visualizador pulsar la tecla D Sample is dispensed Dispensaci n de la muestra 98 apod Programs Part B Programs Parte B Programas UN lt 4 With BLO O Blow out is executed Con BLO Ejecuci n carrera excesiva Start of the mixing cycles O Mn 1004 6 here 6 The counter shows JO g the number of cycles 14 remaining Inicio de los ciclos de mezcla aqu 6 El contador muestra el n mero de ciclos a realizar PA 5 4 The pipette returns to the basic O M gt AU Y position when the key is aki pressed when the pipette is not immersed in the liquid Al pulsar la tecla la pipeta retorna a la posici n b sica si se encuentra fuera del l quido Part B Ordering information Parte B Informaciones para pedido Eppendorf Research pro single channel monocanal incl charging unit con adaptador de carga incl charging unit con adaptador de carga incl charging unit con adaptador de carga incl charging unit con adaptador de carga inc
Download Pdf Manuals
Related Search