Agilent 1260 Infinity Binary LC - System User
Contents
1.2.
3. 4 3
4. 680 pL 6 mL min 10 s
5. 20
6. 2 1 mn i 5 10 pl
7. 80 1260 Infinity LC 89 5 Method and Run Control amp
8. 2
9. OK 1260 Infinity LC 136
10. 1260 Infinity LC Agilent 1260 Infinity LC 1 Agilent 1260 Infinity Binary LC 1260 Infinity Binary LC 2 Agilent 1260 Infinity Binary LC
11. lk 1 2 3
12. Lab Advisor m 78 1260 Infinity LC 4
13. RN A A
14. HPLC
15. A oN 2 before step after step 0 01 min 1260 Infinity
16. 1 C Chem32 Core 1260 Infinity LC 6
17. 1 gt
18. 15 s 6 3 5
19. Not Ready Ready 1260 Infinity LC 85 5 ChemStation DEF_LC M NGC DEF_LC M
20. 1260 Infinity LC 6 2 1
21. 1260 Infinity LC Agilent 1260 Infinity Binary LC 1 1290 Infinity G1316C Agilent 1290 Infinity TCC 10 C 100 C 2 5 ml min 80 C 5 ml min 0 05 C 0 5 C
22. 10 s 10 nL min 52 1260 Infinity LC Agilent 1260 Infinity Binary LC 3 Agilent 2 mL 2 m 5182 0556
23. 7 Oo OK OK 114 1260 Imfinity LC 6
24. 3 5 1260 Infinity 600 bar Agilent ZORBAX RRHT 600 bar
25. i 1 ki 104 1260 Infinity LC 6 0N lt
26. 1260 Infinity LC 61 3 Agilent 1260 Infinity Binary LC 1260 Infinity
27. 10 mm Max Light s 1 0 nL 60 mm Max Light 3 mm 4 6 mm 1260 Imfinity LC Agilent 1260 Infinity Binary LC 3 UV
28. 2 4 6 mm 2 ml min 2 1 mm 2 0 4 mL min 1 2 5 60 1260 Infinity LC Agilent 1260 Infinity Binary LC 3
29. 1 20 min 5 min 700 79 34 3 3 700 20 5 700 700 1 10 58 2 24 700 7400 1570 5830 2 10 2 5 1 27 2 30 2 24 Agilent HPLC 1 8nm UHPLC
30. 1260 Infinity DAD UV 1 1260 Imfinity LC Cat StHD Method lt HPALS HPALS Inieclor Program BinPump gg TCC DAD InstumentCuves BinPump lt v v 4 TCC DAD Show timetable qranh Pressure bar Flow mmin Solvent Ratio amp Solvent Ratio B Left Temperature C Right Temperature C Board Temperature C Dptical Unit Temperature C UV Lamp Anode Woltage Vv HK Apply Cancel Help
31. ADVR Agilent 1260 Infinity 01 ADVR 50 17 Fm DAD1 A Sig 245 10 Ref 360 80 ECHEM32NNDATANNODAMPNOMIX100X21 30000009 D DAD1 A Sig 245 10 Ref 360 80 ECHEM32NNDATANNODAMPNOMIX100X21 30000005 D DAD1 A Sig 245 10 Ref 360 80 ECHEM32NDATAWMIXANDDAMPADVRX21 ADVR30000005D DAD1 A Sig 245 10 Re 360 80 ECHEM32NTNDATA REPROVIAL30 30000023 D mAU 80 PP ADVR I
32. 1260 Infinity LC Agilent 1260 Infinity Binary LC 3 Agilent 1260 Infinity Binary LC
33. oe 5 nm HPLC 1 ml min 4 6 mm 0 2 ml min 2 1 mm 1000 n1 2 1 mm 5 min 600 n1 3 min 1 2 2 um van Deemter NS 3 5 1 MER RE
34. 100 n 1 min lt 2 pL 10 mm 2 ml 7 mm 3 mn
35. 2 CHCls 02 gt 2 COCl2 2 HOM THF 1 EDTA 4 2 THF hr
36. 1 6 6
37. N N N 1 4
38. UV UV Agilent 1260 Infinity G4212B Agilent 1260 Infinity G1314F 80 Hz 64 G4212B DAD G1314F VWD Agilent 1260 Infinity
39. EE gt LOIt MgthodP ITStTHmeTt Check Nethod Secthons to Ed nstsurment Acarisiton Data Ana Bun Time Checklst 23 1260 Infinity LC
40. 2 8 9 2 TCC 1 TCC 1 1 1260 Infinity LC Agilent 1260 Infinity Binary LC 1 1260 Infinity
41. 10 5 mL min Al A1 6 30 mL 7 5 6 8 Off
42. 22 1260 Infinity LC Agilent 1260 Infinity Binary LC 1 1260 Infinity G1312B 1 15 5 kg 34 1bs X 180 x 345 x 435 mm 7 x 13 5 x 17 4 100 240 VAC 10 50 60Hz 5 220 VA 74 W 253 BTU 4 55 C 39 131 F 40 70 C 40 158 FE 95 r h 40 C 104 B 2000 m 6562 ft 4600 m 15091 ft TH 2 TEC CSA UL 1260 Infinity LC 23 1 Agilent 1260 Infinity Binary LC
43. Agilent 1260 Infinity Binary LC NO OpenLAB CDS EZChrom ee ChemStation EZChrom Agilent OpenLAB CDS ChemStation Agilent OpenLAB CDS EZChrom W 78 UV 30 84 1260 Imfinity LC 5
44. 80 Hz 80 80 Hz 1260 Infinity DAD RFID ID ID 20 1260 Imfinity LC Agilent 1260 Infinity Binary LC 1 1200 Infinity Quick Change Agilent 1200 Infinity Quick Changee
45. 1260 Infinity Binary LC 0 12 mm Max light 1 2 3
46. 10 min B 2 lt 95 MW 10 min mi
47. ChemStation ACQ TXT 3 1 10 x 2 ml 4 gt 96 1260 Infinity LC 5 Method and Run Control amp 5
48. 1 Windows 2 LC 3 gt gt Agilent Technologies gt OpenLab gt OpenLab amp ChemStation 4
49. E ChemStation CE EE nn wm T EE 1260 Infinity LC 5 ChemStation 1 2 3 4 1260 Infinity
50. HFIP I 1260 Infinity LC 6 HFIP Al20s A120 HPLC 1260 Infinity LC 6
51. PTFE 1260 Infinity LC 11 1 Agilent 1260 Infinity Binary LC A B A A B 1 12 1260 Infinity LC Agilent 1260 Infinity Binary LC 1
52. OFF
53. amp gt Specify Report Instrument 1 eporting settings Quanttation settings Report mode Use Intelligent Reporting Use Classic Reporting Style Chromatogram output Report style chort n Quantitative result sorted by Signal Potat of pages Time h 00 Sample info on each page Add fraction table and ticks Landscape Add chromatogram output Add summed peaks table Add sample custom fields to sample info Add compound custom fields Response 40 Multi page Landscape Report layout for uncalibrated peaks Pages Separately With calibrated peaks Do not report m a Destination File setting OD Printer File prefix Report Fle Unique PDF file name Screen Cancel Help
54. 69 1260 Infinity Binary LC 10 1260 Imfinity LC Agilent 1260 Infinity Binary LC 1 1260 Infinity G1312B NN CE EN 2 m E 2 1 mm ID 0 1 5 mL min 600 bar
55. 694 GC LC U U U WV Mi MAA MM 5 10 15 20 25 30 35 40 12 BSA ZORBAX RRHT SB C18 2 1 x 150 mm 1 8 m 700 1260 Imfinity LC 2 Power F p F p
56. http www agilent com 112 1260 Imfinity LC 6 ChemStation 1 GUI DEF_LC M
57. HO 10 MPa 100 bar 1260 Infinity LC Agilent 1260 Infinity Binary LC 1 2 Agilent 1260 Infinity GG1312B 1 mL min 600 800 nuL 400 nL 120 nL 0 100 1 1 99 5 nL min 0 15 RSD lt 0 2 1 ml min 0 04 min SD 0 35 2 ml min 10 MPa 100 bar
58. 1 ChemStation amp 2 gt DEF_LC M DEF_LC M Fle RunControl Instrument Method Sequence View Abort Help 9 a NAA N mn nara PS M DEF_LC M
59. 4 6 mm 2 1 mm 4 7 times 1260 Infinity LC 63 3 Agilent 1260 Infinity Binary LC
60. OK gt 6 DAD1 A Sig 254 4 Ref 550 100 DEMO 005 0105 D mu 1960 Infinity LGC 97 5 98 ChemStation
61. 5 1260 Infinity Binary LC ul 120 320 600 800 2 600 bar 2PS 6PT 42 1260 Infinity LC Agilent 1260 Infinity Binary LC 3 2 1 mm
62. 1260 Infinity 1260 Infinity LC 2 1 mm 0 2 um 600 bar 70 x 0 12 mm SS 5067 1551 2 1 1260 Infinity LC 4 6 mm 0 2 um 600 bar 90 x 0 17 mm SS 5067 1553 4 6 mm 3 0 mm 10 5 mL 1260 Infinity LC Agilent 1260 Infinity Binary LC 3 8 2 1 mm
63. 106 1260 Imfinity LC 3 0 4 nm HPLC HPLC Agilent Agilent E 6 7 T
64. 1260 Infinity LC 47 3 Agilent 1260 Infinity Binary LC 5 48 1260 Imfinity LC Agilent 1260 Infinity Binary LC 3 UV 3 0 mm 4 6 mm 5 mL min 4 6 mm 3 0 mm
65. 86 1260 Infinity LC 5 1 View gt Online Signals gt Signal Window 1 Online plot DAD1A DAD Signal A T T r T T 0 E 1 15 2 25 min Change Adjust 2 EGit SignalP lot Available Signals Selected Signals PMP1B BinPump Pressure PMP1C BinPump Flow PMP1D BinPump Solyent Ratio A 3 PMP1E BinPump Solvent RatioB HM1A TCC Left Temperature HMI1B TCC Right Temperature SD DAD1B DAD Siqnal B lt Remove DAD1C DAD Signal C ly Window DAD12 DAD Signal A X axis 3 rn Type acquired axis range 1nn ma drawi zera line auto y adjust Offs
66. 1260 Infinity LC 6 10 20 bar IT 124 1260 Imfinity LC 6 TCC setup Method Adyanced Enable Analysis when front daar open Left Right Not Controled Not Controlled W 400 200 AsDetectorCell AsDetector Cal With any temperature With any temperature a when temperature is within When temperaure is within Column Switching Valve 3 ng ep ea 08 Pot1 gt 2 Stoptime As P
67. DD 1260 Imfinity LC 6 Method of G1367E PPAAN00O05 I gt Injection Mode Injection volume BL Slandard iniection Iniection with needle wash Needle wash Stoptime As Pump No Limit Df 100 lt min mm Advanced lt Auxiliary Draw speed nn mL min Eject speed 1000 mL min Draw position D0 mm Equilibration lime 20 sec Sample flush out Factor 50 times iniection volume Vial wll bottom sensing High throughput FAutomatic delay volume reduction Enable overlapped iniection mple ls Flushed Out sd DF Time 1 n1
68. 3 5 2 1 190 nm 640 nm nn 2 nm
69. A B 4 0 mL min 40 A 60 B 1min 1 min 1 nL Column Eclipse Plus C18 4 6 x 100 mm 3 5 nm 959961 902 Column Poroshel1 120 EC C18 3 0 x 50 mm 2 7 um 699975 302 Column Poroshel1 120 EC C18 4 6 x 50 mm 2 7 um 699975 902 40 C A 250 nm 4 nm 360 nm 100 nm 1260 Infinity LC 91 5 10 A B 0 75 mL min 40 A 60 B 1 2 min 1 min 1 nL SB C18 4 6x 50 mm 1 8 nm 600 bar 827975 902
70. X Y Y Balance 1260 Imfinity LC 5
71. 4 6 3 mm 2 2 1 mm 2 Agilent 1260 Infinity Binary LC AY 1 1260 Infinity LC 37 2 38 1260 Infinity LC 1260 Infinity LC 3 Agilent 1260 Infinity Binary LC
72. 1260 Infinity Binary LC Agilent 01080 68704 Agilent 01080 68704 oc 91 9 92 10 90 1260 Infinity LC 5 9
73. gt amp Method i iniormations Instrument z Method Comments This method runs the test sample to demonstrate how to run an analysis 24 116 gt
74. k te 7 100 A B m DD lll eat 8 k tg 1260 Infinity LC 55 3 56 Agilent 1260 Infinity Binary LC F mL V D B B S 4 5 k 2 5 min F k
75. k k 3 Wr r T 62 1260 Imfinity LC Agilent 1260 Infinity Binary LC 3 S N
76. Zr02 HPLC HPLC PTFE PFA FEP FFKM PTFE PFA FEP FFKM TFE PDD G1322A Agilent
77. 1260 Infinity LC 130 nAU 1260 Infinity DAD 1 100 mAU
78. N 5 nm N 3 5 nm N 1 8 um 12 500 21 000 35 000 8 500 14 000 23 250 6000 10 500 17 500 4 200 7 000 12 000 4 200 6 500 2 100 2 500 11 NM 33 50 67 35 LC MS
79. LAN Configuration E 1260 Infinity LC 4 1260 Infinity LC 73 4 74 74 21 1260 Infinity Binary LC
80. 2 1 mm 43 2 2 A B A2 B1 A1 A2 Bl B2 A B B
81. Agilent Chemstation EZChrom 0L MassHunter Agilent B0 020 00 1 33 mV bar 1 CAN RS 232C APG LAN 1260 Infinity LC 25 1 Agilent 1260 Infinity Binary LC 2 Agilent 1260 Infinity GG1312B GLP Agilent Lab Advisor
82. Quick Change 2D LC 1260 Infinity LC 21 1 Agilent 1260 Infinity Binary LC 1260 Infinity Binary LC 1260 Infinity
83. 76 22 1260 Infinity Binary LC DAD 22 76 1260 Infinity LC 4 ill G1312 87303 ST 0 17 mm x 400 mm S S 1 G1312 87304 SUS 700 mm 0 17 mm 1 32 1 32 pt 2 G1313 87304 ST 0 12 mm x 180 mm S S TCC 2 01090 87610 ST 0 12 mm x 280 mm S S TCC 3 3
84. 6 HiP ALS HiP ALS BinPump TCC DAD O0K RC Net oe ChemStation EZChrom MassHunter
85. Agilent 1290 Infinity Binary LC 0 12 mm Max Lisht 1260 Infinity LC 41 3 Agilent 1260 Infinity Binary LC Agilent 1260 Infinity Binary LC 42 4 42 5 Agilent 1260 Infinity LC 4 1260 Infinity Binary LC 1 120 5 600 800 200 400 270 1 6 3 6 1
86. 0 5 1 1260 Infinity LC 51 3 Agilent 1260 Infinity Binary LC 3 5 Agi1ent 1260 Infinity
87. 1260 Infinity LC 6 11 Y bb i
88. 80 Hz 2 1 3 0 4 6 mm UV 60 mm lt 0 6 nAU cm Agilent Max Light 1100 1200 Agilent 1260 Infinity CO 1100 1200 1260 Infinity 1260 Infinity LC 9 1 Agilent 1260 Infinity Binary LC 1260 Infinity Binary LC
89. 0 002 ADVR
90. 13 2 2 G1312B8 gt 2 sr gt i gt A A B 2 IA 1260 Infinity LC 13 1 Agilent 1260 Infinity Binary LC 1260 Infinity G4225A Agilent 1260 Infinity G4225A
91. RUN M ChemStation GB 02 01 ChemStation OK gt gt gt C Chem32 1 Methods 140 1260
92. LC EMF PC LAN 1260 Infinity LC 71 72
93. Agilent 2003 1 8 nm 2 1 8 nm Agilent 1260 Infinity Binary LC 2 1 2 2 2 STM 2 STM
94. 2 1 2 600 bar 2 2
95. 3 G1316 80002 0 12 mm 1 6 nL 3 G1316 80003 0 12 mm 1 6 nL 4 01090 87611 ST 0 12 mm x 105 mm S S TCC 3 5 G1315 87312 ST 0 12 mm x 150 mm S S DAD 6 5062 2462 PTFE 0 8 mm x 2 m 5 nm DAD 1260 Infinity LC 77 4 on Cb 1 100 mL
96. Agilent Technologies www chem agilent com 1 34 1260 Infinity LC mm 150 100 75 50 30 1960 Infinity LC 3 E 1 LC MS
97. Agilent Agilent Agilent
98. vy 00 T a 120 nl L 13 2 1 mm 1260 Infinity LC 43 3 Agilent 1260 Infinity Binary LC Aux as UV W UV 200 pL 200 mL 5067 1565 0 UV 44
99. 5 10 k k 1260 Infinity LC Agilent 1260 Infinity Binar 3 oN 4 6 mm 2 mL min 60 C 1 5 pL 5 mL min 80 C UV 57
100. 43 13 2 1 mm 2 1 mm UV 200 unL 5067 1565 3 E 3
101. Max Light 10 mm 60 mm Agilent Max Light DAD 190 640 nm 1024 1960 Infinity LC 19 1 Agilent 1260 Infinity Binary LC 4 nm 8
102. 200 bar Ta HPLC gt 1260 Imfinity LC 6 10 SST 1 12 5 pH pH 2 3
103. et ii Me 70 un1 ED PP 1960 Infinity LC
104. CO PP Ww MM 7 T 1260 Infinity LC 79 Lab Advisor EE 1 mL min 2 10 mL 3 1 2 4 5
105. IHStTITTETTIOITVeSIIIHStTITmeTtZ Select curves to nyerlap Instrument Data Curves 34 138 1260 Infinity LC 6 gt RUTTnmMme leCKIiSt mInStTument Z Check Melhod Sections to Hun Data amp cquisition Standard Data Analysis Eustamized Data Analysis Macro Save BLP Data Post Run Command Macro Save Method with Data 35
106. 102 1260 Infinity LC e088 9 6 e 104 107 107 2 115 116 132 139 1260 Infinity LC EE Agilent Technologies 103 6 MNO Agilent
107. 10 5 1260 Infinity LC 4 1 82 8 1 5022 2184 ZDV ZDV 82 8 1 4 2 3
108. 1 119 HiP ALIS setup Method HiP ALS Iniector Piogram BinPump w TCC gg DAD AX Instrument Curves gt Draw default yolume from sample with default speed using default offset uO 0 Legsel 26 OPA FMOC Agilent 1260 Infinity 120 1260 Infinity LC 6 Sampler Sampler Inieclor Program Column Comp 4 DAD FX Instrument Curves
109. DLC HPLC 1260 Infinity LC 110 i02 1290 Infinity pH 12 pH 12 0 pH HPLC
110. Max Light 64 7 7 Max Light Max Light 10 mm V s 1 0 nL G4212 60008 Max Light 10 mm V s 1 0 nL G5615 60018 Max Light 60 mm V s 4 0 nL G4212 60007 Max Light 60 mm V s 4 0 nL G5615 60017 HDR Max Light 3 7 mm V s 0 4 HL G4212 60032 ULD Max Light 10 mm V s 0 6 nL G4212 60038 Max Light G4212 60011 70 bar 1015 psi MOP 150 bar 2175 psi MIP lt pH 1 0 12 5 1 MOP 2 MIP Max Light
111. Quick Change 18 4 18 4 RFID 1 25 C 40 C 0 2 5 mL 1260 Infinity LC 17 1 18 Agilent 1260 Infinity Binary LC 4 TCC TCC 3 8
112. G4225A 14 1260 Infinity G1367E 15 1290 Infinity G1316C 1260 Infinity G4212B 19 1200 Infinity Quick Change 1 299 1260 Infinity Binary LC EE Agilent Technologies 7 1 Agilent 1260 Infinity Binary LC Agilent 1260 Infinity Binary LC 1260 Infinity Binary LC HPLC HPLC UHPLC 2 STM 1260 Infinity Binary LC ZORBAX RRHT
113. K i A i H et 0 0 i 2 CP 3 7 Y 1260 Infinity LC 6
114. Agilent 1200 Agilent 1260 Infinity LC 46 1260 Infinity LC Agilent 1260 Infinity Binary LC 3 Agilent 1260 Infinity 100 pL G1313 68711 400 pL 1400 unL 100 pL 1260 Infinity 500 pL 1500 pL
115. 15 U 45 1260 Infinity LC 3 Agilent i Binary LC 5 lt Agilent 1100 1200 LC 1260 Infinity Binary LC 46 16 2 Agilent 1200 LC Agilent 1260 Infinity af mAU Paracetamol RT shift lt 1 4 Imp J Agilent 1200 Series Imp Agilent 1260 Infinity EE EE EE EE EL EE CEEED ES FL El FE RE EE EL Fl CE CE ES EL FE ET Cs EE A ERT ENE FOE EEL EE ED i EE hE 0 5 10 15 20 25 30 35 min 16 1
116. 63 11 87 14 25 44 43 141 45 49 HPLC 31 63 31 63 61 54 47 64 2 1 mm 43 3 4 mm 45 2 34 17 19 85 72 23 142 23 50 23 23 23 23 23 25 25 24 25 23 14
117. a 1260 Infinity Binary Plmp 4 0 ml min A B B B 60 1 min 1 min b 3 a 1260 Infinity 1 0 n1 6 s Qr 7T Qr TT E OK Qr Qc 94 1260 Imfinity LC 5
118. pH Zorbax SB 90 C 2 2 2 2 10 1 1960 Infinity LC 49 50 3 Agilent 1260 Infinity Binary LC
119. 2 k 2 C18 C8 en 54 1260 Infinity LC Agilent 1260 Infinity Binary LC 3
120. pgH E Agilent i UHPLC 7 F f MA rE 1960 Infinity LC
121. 4 LC 3 4 1 14 1260 Imfinity LC Agilent 1260 Infinity Binary LC 1 1260 Infinity
122. E 5 1 mm 118 1260 Infinity LC ADVR 6
123. Agilent 1260 Infinity 120 nL AN ED CN G1316 68744 0 12 mm 1 6 nL 1 5 nL UV 64
124. 59 19 PW 0 30 80 Hz PW 0 33 PW 0 42 20 Hz PW 0 67 10 Hz PW 1 24 5 0 0 1 0 2 03 04 G 19 UV 60 6 LC 1260 Infinity LC 59 3 Agilent 1260 Infinity Binary LC 6 80 Hz 0 300 2 25 60 40 Hz 0 329 2 05 55 20 Hz 0 416 1 71 45 10 Hz 0 666 1 17 29 5 Hz 1 236 0 67 16 80 Hz 20 Hz 30 30 40 70
125. G1367E 1260 Infinity 600 bar Agilent 1260 Infinity Binary LC 2 384 768 1
126. MN TCC 300 mm 100 mm 2 1 6 n1 TCC 4 HPLC 3 n 1 6 1 TCC 1 2
127. 49 52 54 61 66 69 70 7 83 84 85 Method and Run Control amp 30 98 1260 Infinity LC 6 103 104 107 112 1960 Infinity LGC 113 6 1260 Imfinity LC 1260 Infinity LC e e088 9 1 vee Agilent 1260 Infinity Binary LC 9 Agilent 1260 Infinity Binary LC 8 10 1260 Infinity G1312B 11 1260 Infinity
128. 8 nm nm 1260 Infinity LC 128 nm
129. Flow Adyanced Timetable empty Hm ml min function centric Solvents TimelImin 7 A BE am Lil aa non 1000 nn nno 400 00 B Pressure Limits Min DO0 bar Max 40000 bar Stoptime Posttime As lniector No Limit Off OO min min Add Remove Clearal ClearEmp Cut Copy Paste shitTimes min ki Show timetable graph OK Apply Cancel Help 27 5 MG 4 mL min 1260 Infinity LC 122 2 A B
130. 3 5 ml min 10 4 5 7 5 6 3 5 m min 5 7 1 H 1260 Infinity LC 81 4 8 1 7
131. 1 1260 Infinity LC 53 3 Agilent 1260 Infinity Binary LC 2 1 D C D Pour e Oo Rs R N
132. Max Light HDR G4212A DAD G4212B DAD 2013 3 4 HDR LC MS G4212 68001 Agilent 1200 Infinity 1260 Infinity LC 65 3 Agilent 1260 Infinity Binary LC 2
133. 40 40 Agilent 1260 Infinity Binary LC 42 2 1 mm 02 43 3 4 6 mm 45 47 49 51 52 54 57 61 62 64 66 LC EE Agilent Technologies 39 3 Agilent 1260 Infinity Binary LC 40
134. RM 20 H HX NN 0 2 nm 24 48 h gt 50 mM
135. 0 02 b c 0OK DAD gt IS0 1 M ChemStation DEF_LC M 10 min Qc ran Qr E 0OK 9 4 Qr TT 7 Y Qr TT 1260 Infinity
136. 32 9 J 32 10 TO Et 7 Y 9 R f oy 4 3 f N 2 f k 1 20000 40000 60000 80000 N 1 05 1 10 1 15 1 20 Qa 5 10 15 20 k 10 R 32 1260 Infinity LC UHPLC 2 Poroshe11 E Agilent 140 ZORBAX 1 8 nm RRHT 14 4 6 mm
137. 9 10 8 PE 2 0905 1420 HPLC HPLC 5 2 82 1260 Infinity
138. 3 Agilent 1260 Infinity Binary LC LC 4 5 1260 Infinity Binary LC 6 1260 Infinity LC 3 Agilent 1260 Infinity Binary LC 7 Agilent 1260 Infinity Binary LC 8 10 9 27 28 34 37 Agilent 1260 Infinity Binary LC 39 40 47
139. 30 A B UV amp GUI gt
140. 7 91 9 92 10 10 min 8 1260 Infinity LC 93 5 DEF_LC M 1 2
141. 0 1 ml min 3 0 mm 0 2 mL min 4 6 mm 0 4 mL min 5 9 10 10 1 5 ml 11 00 130 bar A uh 2 oe 6 mm 20 1 8 pm 4 6 2 1 mm 0 2 gm 1260 Tnfinity LC 67 3 Agilent 1260 Infinity Binary LC 68 1260 Infinity
142. 250 nm 8 nm 254 nm 12 nm 1260 Infinity LC 6 SignalDefails instramentz Available Signals DAD1 4 Sig 250 100 Ref oft Insert Row Append How Delete R
143. 0 1 2 3 4 5 6 74 2 17 50 17 2 1 mm 0 6 mL min 1260 Tnfinity LC Agilent 1260 Infinity Binary LC 3
144. Lab Advisor DVD Agilent Lab Advisor ChemStation Agilent Lab Advisor Windows Agilent Lab Advisor Agilent
145. pH 24 23 24 100 100 11 24 14 25 25 25 19 44 47 43 25 40 40 42 40 40 98 25 114 26 14 1 23 1260 Infinity LC 23 23 72 89 24 11 45 23 80 64 58 57 60
146. 59 32 36 98 54 54 23 23 23 32 24 37 11 114 96 94 113 13 81 81 86 1260 Infinity LC 115 11 114 24 24 11 24 24 101 32 143 WWww agilent com Agilent 1260 Infinity AN LC 5 Agilent Technologies 2006 2008 2011 2013 Printed in Germany 02 2013 G1312 96303 Agilent Technologies
147. LC 99 5 2 MP 1 se Calibration mi Signal Purify le Spectrum IAM Repors Stor a EDAD1A Sig 254 MO 005 0105 D AS I Al NY fad a DAD1 A_ Sig 254 4 Ref 550 100 DEMOI005 0105 D mu Melhod ManualEvenls 100 Initial Events For All Signals Integration Events Value Tangent Skim Mode Standard Tail Peak Skim Height Ratio 0 00 eo Front Peak Skim Height Ratio 0 00 Skim Valley Ratio 20 00 Baseline Correction Advanced 40 Peak to Valley Ratio 500 00 1 Specific Events For Signat 5 8 iic Events For Si DAD Delaul gt 5 6 i min R Time Tntegration Events Value Time Area Height Width Area Symmetry Initial Slope Sensitivity 5 0 747 301 105 9 0 0452 29 738 0 718 Initial Peak Width 0 02 1023 275 9 76 8 0 0539 27 257 _0 648 Initial Area Reject 5 3 2 571 178 2 26 9 0 1013 17 604 0 634 Initial Height
148. 100 C 1290 Infinity 1 6 nL 1 5 nL 10 C 100 C 1290 Infinity Quick Change 8 1260 Infinity LC Agilent 1260 Infinity Binary LC 1 EE 0
149. 2 Agilent 1260 Infinity GG1312B 2 yx 24 0 001 5 mL min 0 001 mL min 0 05 5 0 mL min lt 0 07 RSD lt 0 02 min SD 1 10 nL min 0 60 MPa 0 600 bar 0 8700 psi 5 mL min lt 2 1 3 lt 0 3 MPa 3 bar 1 mL min gt 1 MPa 10 bar 147 psi lt 5 2 1 0 12 5 pH lt 2 3
150. 4 5 6 1260 Infinity LC 15 1 16 Agilent 1260 Infinity Binary LC 7 8 9 10 7 8
151. 500 Da Van Deemter STM STM 2 ml min 4 6 mm 0 4 ml min 2 1 mm k
152. LC 95 5 ChemStation 2 ChemStation 1 2 gt
153. 33 PDF 1260 Imfinity LC 100 40 PDF PDF OK 1260 Infinity LC 137 6
154. LC 125 126 5 C 100 C Agilent ZORBAX RRHT StableBond 10 C 0 15 C 12 15 C E TCC 3 2 6 2 2 10 8 9
155. UHPLC UHPLC HPLC 400 bar HPLC i Agilent 1200 bar UHPLC 1290 Infinity LC 28 1260 Infinmity LC HPLC van Deemter 29 6 H HETP d u A B C A B
156. 1 7 nm Poroshel1l 50 2 Poroshe1 3 i ad i Agilent Infinity Z0RBAX 1 8 nm RRHT 3 5 5 um Z0RBAX E 1 8 nm 1960 Infinity LGC LC UHPLC ZORBAX 33 2
157. MDS 1260 Infinity LC 6 Binap Pump Sampler Sampler iniector Program gg Coum Comp DAD Instrument Curves Signals Adyanced Use Wave Band Reference Rieference Spectrum Signal length width Wavelength Bandwidth Store Nane Signal amp V 2540 40 3500 1000 nm Signal B 00 40 vy 3600 1000 nm Riange from 190 0 to 400 0 nm Signal C 2140 40 3600 1000 nm Step m Signal D 2300 40 3600 100 0 nm lt Threshold 10 0 mall Signal E C4 40 vii3600 100 0 nm SignalF 2730 40 3600 100 0 nm Analog Output Signal G BOO 4 0 360 0 10OO 1 nm uput 1 Signal H 250 0 4 0 360 0 100 0 nm Zero Offset Peakwidlh Attenuation 1000 mal gt 010 min 2 s response Ime 2 5 H CO SB Margin for negative Absorbance Stoptime Posttime mu As Pump lniector Off Autobalance Lamps on required for acquisition 100 mn 1 00 mn Frerun 9 UV Lamp Pastrun Timetable empty Show timetable graph 29
158. Vespel Agilent pH1 10 DMS0 THF PE Agilent 1290 Infinity 1260 Infinity UHMW PE PTFE pH1 12 5 THF
159. Z0RBAX 9 Agilent 1260 Infinity Binary LC NE SPP 0 5 nm 2 H 15 150 mm SPP i STM 2 1 3 0 g Poroshe11 EC 5 0 3 D 1
160. 18 18 PCCO 2 1 mm 1260 Infinity LC 57 3 58 Agilent 1260 Infinity Binary LC 4 6 mm 45 15 2 1 mm UV 43 13 44 14
161. i 20 25 C 68 107 PEEK PEEK pH1 12 5 AN THF DMSO gt 1 gt 10 gt 10 i PEEK a Agilent DuPont
162. 1 8 nm 1 8 nm 1 2 1 3 1 2 1 3 A C 0 0045 0 0040 0 0035 0 0030 0 0025 0 0020 HETP cm 0 0015 0 0010 0 0005 0 0000 0 0005 0 0 0 2 0 4 0 6 0 8 1 0 2 1 4 1 6 uo
163. Agilent Technologies Agilent Technologies Inc 2006 2008 2011 2013 Agilent Technologies Inc 61312 96303 02 2013 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Agilent OpenLAB CDS A 01 04 Windows Microsoft Corporation o
164. EMF Early maintenance feedback 500 L min 1 26 1260 Imfinity LC 1260 Infinity LC e088 2 e ee 28 34 37 Agilent 1260 Infinity Binary LC EE Agilent Technologies 27 2
165. 40 C 250 nm 4 nm 360 nm 100 nm 4 1 nm 92 1260 Imfinity LC 5 1 A HPLC B HPLC 2 3 5 mL min 4 5 HPLC 5 min 6
166. Amount Tn Multiplier Dilution ok cacem Hep 2 3 PDF PDF D ChemStation Windows File Explorer 4 ChemStation ChemStation ChemStation
167. LC 6 B 75 95 0 00 min 0 00 min
168. 10 mm V s 1 0 unL G4212 60008 2 1 mm ID x 2uL pw 0 04 min 0 1 mL min 0 04 min x 0 1 mL min 0 004 m 4 nL 64 1260 Infinity LC Agilent 1260 Infinity Binary LC 3 Max Light 60 mm V s 0 nL G4212 60007 kN LOD 3 1 Max Light ULD G4212A DAD G4212B DAD 1290 Infinity 5067 5189 Fr
169. LC 1260 Infinity LC e088 ee 5 84 85 85 86 87 89 Method and Run Control amp 90 90 94 96 98 99 100 101 1260 Infinity Binary LC EE Agilent Technologies 83 5 Agilent 1260 Infinity Binary LC
170. 1 2 3 4 DAD 5 21 1260 Infinity LC 1 G1312 87303 1 G1312 87304 2 G1367 87304 2 01090 87306 3 G1316 87300 4 G1315 87311 5 5062 2462 5 5062 8535 ST 0 17 mm x 400 mm S S SUS 700 mm 0 17 mm 1 32 1 32 ST 0 17 mm x 250 mm S S TCC ST 0 17 mm x 380 nm S S TCC ST 0 17 x 90 mm S S TCC ST 0 17 mm x 380 mm S S DAD PTFE 0 8 mm x 2 m DAD 1960 Infinity LGC 5m 4 75 4
171. 14 2 1 mm UV 320 nl 200 nl 14 UV 2 1 mm 44 1260 Tnfinity LC 600 800 ul Agilent 1260 Infinity Binary LC 3 3 4 6 mm UV 3 mm 4 6 mm 2 1 mm 2 5 4001l V 3 4 6 mm
172. C H 29 6 van Deemter a 40 RIN 1 H nin ue u 6 van Deemter 1260 Infinity LC 29 30 7 van Deemter 3 5 nm 5 0 nm
173. gt Specify Repnrt Instrument 1 1 ings Quantitation settings Report mode Use Intelligent Reporting Use Classic Reporting Style Rieport style Short Huantitative result sorted by Sample info on each page Add chromatogram output Add sample custom fields to sample info Report layout for uncalibrated peaks Separately With calibrated peaks Destination File setting Printer Screen Ei Z Unique PDF fl name File prefix Report Add fraction table and ticks Add summed peaks table Add compound custom fields Do not report Chromatogram output Portrait Landscape Response Multi page Landscape 1 Pages of pages 100 3 Signal gptions 1260 Infinity LC 101 5 Specify Repnrt Instrument 1 Reporting settings Quantitation settings Calculation mode Calculate uaa Based on Area ISTD carreclion Use multiplier and dilution factor with ISTDs Calculalion factars Use sample data From Data File Compaund ISTD Amount
174. 3 ChemStation EZChrom 3D 8 A A
175. cm 7 van Deemter 1260 Infinity LC 2 HPLC pH gH 8 HPLC 1960 Infinity 1C 31 3 3 N a k
176. Infinity LC ADVR 50 D DEF_LC M 113 P pH 24 V van Deemter 29 11 112 24 14 24 24 25 104 104 28 106 Lab Advisor 71 70 70 112 50 87 7 41 40 40 37 37 2 34 49 66 34 11 1260 Infinity LC 62 61 114 116 51 52 84 25
177. LC 1260 Infinity LC e900 ee e 4 e e 8 70 72 72 72 73 78 EE Agilent Technologies 69 4 70 1260 Imfinity LC 4 Agilent Lab Advisor Agilent Lab Advisor
178. Reject 1 4 5 856 257 1 16 9 0 2255 25 401 0 676 Inital Shoulders OFF 2 5 3 5 0 02 6 7 A 8 100 1260 Tnfinity LC 5 1
179. STM Agilent 1260 Infinity Binary LC 1260 Infinity 0 05 5 mL min 600 bar 120 nL 2 1 mm 4 6 mm 1260 Infinity UHPLC HPLC 1260 Infinity 0 1 100 nL
180. A 100 B A B 60 A i A 40 B
181. G4212B 1260 Infinity DAD LC Agilent Max Light Max Light 1024 5 DAD
182. b TCC a 1290 Infinity TCC 40 C b 0OK TCC a 1260 Infinity DAD A A 254 nm 4 nm 550 nm 100 nm
183. et 10 Fraction Gollector Method Settings T Show fraction collertion ticks Use method settings Apply to Method OK Cancel Help 1260 Infinity LC 87 5 3 4 O Apply to Method 90 min
184. ow Signal Description Align Peak1 Peak 2 Align Window 31 1260 Infinity LC 133 134 i 0 gt EE ion A Signal My p TF 3 wg Report Short DAD1 A sig 242 0NE MIX 008 D gt lt GA P Method ManualEyents Initial Events For All Signals Integration Events Value Tangent Skim Mode Standard Tail Peak Skim Height Ratio 0 00 Skim Valley Riatio Peak to Valley Fiatio 500 00 Specific Events For Signal DAD Default 5 Time Integralion Eyents Value Initial Slope Sensitivity 5 Inital Peak width 005 Initial Area Reiect 5 Initial Height Reject 1 Initial Shoulders DFF 32
185. ump Iniector 100 mn 28 RTL TT RS CE 0 6 0 15 cm 2 60 C 1960 Infinity
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