Kapa Library Quantification Kit 操作方法ガイド
Contents
1. Template Dilution Factor nM 10x106 P0 I Template Dilution Factor Template Dilution Factor nM x 5x109 D 8 3nM X 20 210xX106 Dilution factor MM 280Xx108 18Lu Template Dilution Factor 457 1 456 18 emPCR2. KapalLibrarVIQOuantification Kit Fon Torrent qPCR KAPA Library Quantification Kits NOrm FIUOrO Std 1 Std 4 StH GE Std5 Std3 Std6 Lib x1000 Lib x2000 Lib x4000 Lib x8000 10 Ct 1 Cycle Step1 qPCR CVOle IIIIHIIIIIIIIHIIIIIIIII Step2 4 0 Log Concentration Step3 qPCR Ct Step4 Ct Kapa Efficiency Calculator Step5
3. 8 8 KapalHibrarVIQuantification Kit MKlon TorrentrW 6 qPCR qPCR qPCR qPCR qPCR Ct 95 C 5 95 C 30 5 60 C 45 Template Dilution Factor 1 3 emPCRH 1 PCR 6280x109 18
4. Ct 3 3 3 4 AF td 00 6 Y 3 36 24T KapalHibrarVIQuantification Kit MKlon TorrentrW 1 qPCH gt 1 qPCR tab txt Ct Dilution factor qPCRH Export Excel Ct 3 Kapa Efficiency Calculator FCREfficjency Exg FCREfficlency alculatnr
5. a gt a 2 1 1 1 2 KapalLibrarVIQuantification Kit MKlon TorrentW 4 STD _ NTC Non emplate Control gt n 3 6 Lib 2 1 3 7 8 9 10 11 12 STD STD STD Lib Lib Lib Lib Lib Lib 1 x500 x500 x500 x500 x500 x500 STD STD STD Lib Lib Lib Lib Lib Lib 2 2 x1000 x1000 x1000 x1000 x1000 x1000 STD STD STD Lib Lib Lib Lib Lib Lib 3 3 x2000 x2000 x2000 x2000 x
6. Export KapalLibrarVIQuantification Kit MKlon TorrentrW 4 Excel Template Dilution Factor qPCR Template Dilution Factor 153bp DNA bp Template Dilution Factor qPCR Template Dilution Factor Template Template Dilution Factor 0 Dilution Factor 3 2 53 A 5 Trg000 YX 000 000 AVg _Avg fragment length_ length Template Dilution Factor
7. 83 reaction efficiency 90 11096 3 3 10 Ct 3 3 3 4 gt Ct 3 reaction efficiency 9096 gt 1109 qPCR reaotion efficiency lt 909 gt 11096 qPCH qPCR
8. 1 gt 2 Shear sample LiDrary Library Library DIN Preparation Amplification Quantification US AUG Library Library Library Quantification of Quantification Shear sample Library Library Library BIN Preparation Amplification Quantification 1 1 emPCR Template Dilution Factor Template Dilution Factor emPCR emPCR 2 g2e http www n genetics com 112 0004
9. KapalLibrarVIQuantification Kit MKlon Torrent 5 qaPCR lt gt KAPA SYBRR FAST qPCR Master Mix e Primer Premix T1241 ROX PCR 4 NTC 4Al 20ul 1 398 18 6 NTO6 45 24 18 NTC3 9 8 86 NIO5 4 69 48 18 NTC3 5 6 60 NIO5 06 0 TU I6 NICO C
10. Web i BE 3 DNA AgilentBioanalyzer 2100 Expert Region Table Average Size bp of Tet hverage Soe pei Soe dsrtutonn CX Wd Conc Mer cey 9 5 95 re a eT ba I NM 3 Kapa Efficiency Calculator reaction efficiency qPCR qPCR Efficiency Calculator gt 10 10 2 2 reaction efficiency Ct 3 Standard Curve to Calculate qPCR efficiency Efficiency of the reaction is 77 8 Slope ofstandard curve is 4 00 y dx 17 Calculate Calculate 1 reaction effclency
11. 1 4 14 18F Tel 03 3813 0961 Fax 03 3813 09862 604 8277 565 GF Tel 075 257 5421 Fax 075 257 5422 10P1306
12. 013 9 6 rev3 KapallIDrarViQOUuantificationlKitl IonalorrentW KK4827 KK4838 KK4847 KK4857 PrimerITA 5 CCATCT CAT CCC TGC GTG TC 3 Primer IT trP1 5 CCT CTC TAT GGG CAG TCG GTG AT 3 qPCR Primer Mix 10 q PCR dSDNA 1 500 qPCR 15 qPCR 30 qPCR N 4 Kapa 1 6 PCR 50ml Tween 50 ml 10mM Tris HCI pH8 0 10ml 1 5ml QPCRH PCR Plate QPCRH 1 qPCR PCR 50ml 1
13. Template Dilution Factor 6 PCR nM Template Dilution Factor nM 610X109 60u Template Dilution Factor Template Dilution Factor nM X 5x109 D 8 3nM X 20 1 210xX108 3 PCR dsDNA ul Template Dilution Factor qPCR 1 3 1 Template dilution factor 2 nM 3 dsDNA otd 1 ie 0 083026943 50 000 000 td 2 0 3 0 008302694 5 000 000 Std 3 0 0G 0 000830269 500 000 Std 4 0 003 8 30269E 05 50 000 Std 5 0 0003 8 30269E 06 5 000 Std 6 0 00003 8 30269E 07 500 Template dilution factor emPCR 6280X108 18 qPCR
14. 0ml 1 C 4 Primer Premix KAPA SYBR FAST qPCR Master Mix 12gul x PCR 4ul x 3 qPCR 2 16 1 NTC 41 20ul
15. 2000 x2000 5 6 4 6 3 STD STD STD Lib Lib Lib Lib Lib Lib 4 4 4 x4000 x4000 x4000 x4000 x4000 x4000 STD STD STD 6 sTD sTp us 6 X 2 500 1000 2000 4000 1 5ml 1 4 SUA EOOLUL e004 8200 lual 800 800l 800l 500 500 1 amp 499 l 500l 200 200 200 l 1 lal Xx500 x1000 x 000 x4000 1000 4000 500 200ul 1000 3 G000 4 4000
16. 5ml X4 q 0ml 0 W 8 1 1 KapalHibrarVIQuantificationKit MKlon TorrentrW 3 1 10mM Tris HCI pH8 0 0 ei 50ml 10mM Tris HCI DH8 0 10ml Tween20 5ul G 8 qPCR a CS 5ml mW TP 6 GE NB ME Instrument ROX Reference dye ABI5700U 7O000 7300 7 700 900HT stepOne StepOnePlus OE NB jj A TM AB 7oOO0 VIIA XY 2 00 stratagene Mx3000P MxSGO0O05PIM Mx40009 ROX 3
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