pBApo-EF1α Neo DNA - Clontech Laboratories, Inc.
Contents
1. 2 AIRES BamHI EcoRI Hindili 1 Cgl EcoRV 2 Sees Gee ene DNA v201003Da TaKaRa B943433972. vitro in vivo Adenovirus Expression Vector Kit Dual Version Ver 2 6170 BamH xbal pBApo EF1a Neo 5 167 bp 10mM Tris HCLpH8 0 pBApo EF1 a Neo DNA 3243 ImM EDTA ORF 20 C 2 _ NE ee OHR 5 167 bp f aaa R 7 a 1 dideoxy
3. Vector map for pBApo EF1 a Neo DNA EcoRV Cla SV40 polyA EFla promoter BamH xbal pBApo EF1 a Neo 5 167 bp SV40 promoter Neomycin resistant gene as a selection marker of pBApo EF1 a Neo DNA Cat 3243 Note This product is intended to be used for research purpose only They are not to be used for drug or diagnostic purposes nor are they in tended for human use They shall not to be used products as food cosmetics or utensils etc Takara products may not be resold or transfered modified for resale or transfer or used to manufacture commercial products without writ ten approval from TAKARA BIO INC If you require licenses for other use please call at 81 77 543 7247 or contact from our website at www takara bio com Users must contact TAKARA when they plan to use this product for purposes other than research use The viral supernatants produced by adenovirus vector could contain potentially hazardous recombinant virus depending on an insert gene Due caution must be exercised in this production and handling of recombinant adenovirus Please follow the guideline for experi ments using recombinant DNA issued by the relevant authorities and the safety committee of your organization or your country in using this product Takara is not liable for any accident or damage caused by the use of this product Produced by TAKARA BIOTECHNOLOGY DALIAN CO LTD v201003Da pBApo EF1 a Neo DNA Code No 324
4. 3 Shipping at 20 C Size 20 ug Stored at 20 C Lot No pBApo EF1a NeoDNA EcoRV chal EcoRI SV40 polyA EF1 a promoter pBApo EF1 a Neo DNA EF1g promoter A ORF microRNA ORF A SV40promoter
5. pBApo EF1 a Neo DNA Code No 3243 Size 20 ug Shipping at 20 C Stored at 20 C Lot No Concentration 0 5 ug ul Volume 40 ul Regarding protocol please refer to the product manual supplied with this vector Description pBApo EF1 a Neo DNA is a simple expression vector for mammalian cells This vector has a promoter from human elongation factor 1 a gene EF1 a promoter PolyA signal from Thymidine Kinase of Herpes Simple Virus and neomycin resistant gene as a selection marker By inserting the ORF of target gene into MCS the expression plasmid for the target gene can be constructed This vector can be also used to express the transcript of pri microRNA In addition it is easy to transfer the cassette of promoter ORF PolyA signal from this vector to adenovirus vector especially to pAxcwit supplied in Adenovirus Expression Vector Kit Dual Version Ver 2 Cat 6170 Since adenovirus vector has high infection efficiency and wide target cell spectrum it is suitable for in vitro and in vivo gene transduction Form 10mM Tris HCl pH8 0 1mM EDTA Preparation Purified by ion exchange column Shelf Life 2 years from date of receipt under proper storage conditions Chain length 5 167 bp Purity 1 Confirmed to maintain the promoter region and Cloning Sites by dideoxy sequencing method 2 Shown to be cleaved at a single site by BamH EcoR and Hind Ill and at two sites by C a I and EcoRV
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