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1. PC 17 QO2. 1 a UV B 2 m 3 3
3. O C jejuni Cco7 2 IIA 2 NS 17 Kt ss m Lo u ol LAMP eg ER BE O DNase RNase
4. LA 320C 11 O LA 320C O 8 8 u z _ Ser 11 LA 320C 32
5. a 1 JE DNA a y C Kt Don dee E 2m Zar D u ol Loopamp LP La Tara nr ar aH cn act OS LAMPIE AR A e E Me nA EEE SMR fe E DE 6 L E T 3 Loopamp au 43 Loopamp LAMP
6. a a 1 0 55 RERHR o Ze 1 B 100 400nm UV A 315 400nm UV B 280 315nm UV C 100 280nm T UV UV C 240 280nm DNA DNA a U
7. 8 E a re 8 ne E a
8. 8 8 B Z LoopampW J L A PA Hk u Sana Min C B N c FM J D LAMP 6 LAMP D RAIM D F 7 DNA LAMP 3 Loopompr Sa L LA S H 4 T A 7 ie ar Fe T RESIITIL JA IV ER UV
9. a I DNase RNase R RNase DNase RNase a 8 8 RNA a DEPC DNase RNase a RNA
10. 1 621 LAMP DNA 1 18 19 C 8 age B
11. A BC D D CB A 2DNase RNase E DNase RNase zz K gt AT Zur 1 Notomi T et al Nucleic Acids Research 28 No 12 e63 2000 2 Nagamine K et al Clin Chem 47 No 9 1742 1743 2001 3 ZR 23 2000 4 Mori Y et al Biochem Biophys Res Commun 289 No 1 150 154 2001 5 TH h 26 EIHA ST4HYWF F SHEICI D BERGE 2003 6 Kohker S et al Infect Immun 58 No 6 1943 50 1990
12. B tiD e pj D 18 LAMP BREI A 20 A E B B C Rg Rg C D B 8 A B C D
13. 3 aa UV a a c DNase RNase 3 E REET RNA
14. SH YE dm Je VI ARTE D O 13 NN8 0 IV C E DS FALLIR LAMPA Iai a LA 320C 14 a Abs a a
15. o a u i LC 3 8 8 E 3 8 8 E g RA C gt A Ii I
16. 1 25g 100mL 42 C 24 2 DC O IAAI Extraction Solution for Foods EX F 95C 5 1M Tris HCI LAMP Loopamp S LAMPRIS HS J UGA BE LoopampW LAMP 65C 1 NT 80C 2 L 25g 100mL CEM 42 C 24 50 u L LAMP 1 2xReaction Mix 12 5uL Primer Mix Cam 2 5uLl Distilled Water Aul Bst DNA Polymerase TulL EX F 50L 95C 5
17. T a P 21 LAMP 5L H a O
18. Loopamp X LAMP Loop mediated Isothermal Amplification 2 24 2 O Campylobacter jejuni Campylobacter coli LAMP 1 O 1 e Loopamp 1 1
19. RT 160C 12 O D O Start Heatl 16 k en ee 12 RT 1 ee 2 BIF
20. EREU ISE DI J gt M lt Mn DNase RNase 3 lt IL P 22 6 7 A 8 B 8 a 3 C a B
21. F u N RAI VAA rat IV 0 559 v y E 1 lt DNA RNA
22. 2 BSL2 P 21 10 P 28 D a 5 11 000 gm L 1 10gL QO 2 gt 20 u L 20 200 u L 200 1 000 10 4 4 D 20 u L 200 u L 1000 u L PE XY hic O 1 5mL R Loopamp8 LAMP 8 12 8 0 5 1 5mLH
23. UV 1 a UV a UV lt DNA RNA DNA RNA a DNA RNA a DNA RNA DNA RNA UV
24. 1 2 XReaction Mix RM 12 5 u L Primer Mix Cam PM Cam 2 5 u L Distilled Water DW 4u L Bst DNA Polymerase 1 u L a 20 21 1 X3 T lt 22V 10 A DO Positive Control Cam PC Cam Distilled Water DW D LoopampW 20 L C 2
25. Aa D a lt 477 BE B C I a a ERAGE P 22 ne V 8 O a 0
26. 4 5 O 4 6 O 1 5 O EO Kt Eh 5 N EM 1 5mL 5 9 O O Ra 7 i A EN 0 2mL 8 5 O 8 5 40 O 5 O OF 5 4 O O OX Ila 5 43 O O O NR 11 12 e AMP 3 TE I P 21 3 Loopamp I me ER oe De se iD II 4 D
27. D C B A a Loopamp LAMP 2006 4 a MIE 7 15272 ZA EH Ae NTARE Ii C DNA RNA DNase RNase UV a 22 L Lb A a a A B C D R 3 DA EL D C B A Bun PHIX DB D 1 A
28. RT 160C 15 e Abs 3 2 CT 8 a LAMP 0 1 2 o _o_ lt 50 60 0 10 20 3040 50 60 0 10 20 30 40 13
29. 504 PMN n RAF IIALEHY TCHHP G 18 5116 Z 6 L CZE D 95C 5 1M Tris HC1 pH7 0 Tris 0 2 000Xg 60 9 LAMP 6 2 Syl 2 ge va ri LI g TR A D 20C D 1 5mL
30. ARE ET Are R ef ir BD iR 132 At 22 mdi 22 VP D O 12 1 2 Campylobacter jejuni VA F C jejund Campylobacter coli VAF C col C jejuni EC coli C jejuni 5 LAMP C jejuni nn gene C coli i R FF BH ZT Aspartate kinase gene 2 C jejuni C co7 7
31. u O PAR TC Fa 7 WEE 3 LAMP 8 aE SpF
32. 2006 4 hR l f J dr Sue gt Jis SN L ma Z th H L EWES I 9 B B un 7a 7 _ D 25g 2 100mL 100mL O 10mL 42C 24 sta 42C 24 B HOF 50 ER 7 1 5mL Extraction Solution for Foods EX F 50
33. a a A B C D tn J D C B A a P Dn E 2 20 u N 20 a A a T 2 4 lt RE tak LT Extraction Solution A QA Extraction Solution WG AAIE TER
34. 14 LA 320C V d RA F R 1 G 23 E K a 000000 08 15 RT 160C ee 57 st 142 m ni a En 2 aid 7 FIT SE an 1 IR MARAI 1 lt dar HEL JE SE g FR CEM 100mL O 10mL O 42 C 24 10mL Lk E B 50uL ol Ester Solution for Foods EX F 50 u L gt LT MM E O _ O 95 5min ltest F _ 2xReaction Mix RM 12 5 u L C f AM Cam 25 uL cq OL 1M Tris HCI pH7 0 Tris a o e IN O Bst DNA Polymerase TuL O RH 7 a D 01 SE 204 L tube C mid 2 000xg 60sec Loopamp s A o Sch gt O gt 14 5 L tube 5ul tubbe 5 u L tube Positive Control Cam PC Cam
35. da DEPC RNase a RNA 8 DNA RNA UV WEE
36. 0 275 v v 2 750ppm 5 g DNA 5 g yeast tRNA 10 0 55 v V 5 500ppm 30 DNA PCR 12 DNA RNA L ZEN rear ehr zH TE ML T ARERI TOO EER u S N I E 5 500 ppm 0 55
37. a E 8 8 8 gt P 21 D a 3 NN Z Le PH P v2 d ia 9F IV LAMP DNA
38. FRAR 1 8 OQ n Extracti a Solution Foods a J RE ehren i a a
39. QO DNase RNase O au o nenn EZL Atta yT R ENL ET O ROF TIS 27 7 H U7 L CE H PHELES DNase RNase D O P 3 B CD ee a 2DNase RNase 2DNase RNase
40. MM LAMP Li O D i gt er PP E ae y EA EFXIBLL EFA I 4 0 5596 DNA aB DNA RNA E DNA
41. 20 lt 200 L 5 0 O EO Te Y 1 2 X 10 EEE r200 1000 11 BED PB 5 2 O O O Be 1 OD 5 O O DNA o 1 LAMP Loopamp 5 9 O m m 1 50 O 4 Loopamp 5 O E 5 6 O EO EO 5 O O O 4 O 4 3 O
42. 5 u L tube Distilled Water DW LAMP 25 L Loopamp O O 65C E L C LAMP 65 C 60min 80C 2min 110L f NUN Y R ms o E 777 L A M P EE x IE pamp WEE VJ GE P at 20064A LS Loopomp rr j l DATE ASSAY Profile Lot No SHEET No B5 B6 D5 D6 Position Profile D7 D8 EE gt gt gt D gt gt u B J ww 0 NIO OO PR 10 N gt 00 CO w 1059 01142103 N J SI 3 3 18 3Loopamp LAMPE MARAG S E Mita MH Ah de 6 gt D C T M f E MO B L ET Loopamp LOPID Ta Ai oe rn ot rend ao Arte CORTA Aa Eea fE S R T 1 L Gr gt UPA aa oz u ul 16
43. C 3 Loopamgp Yia F zu z Iy e O a 2 u ir eh rer iira k BASED A gt MP 4 r L a 73 7 PREE NEA A AIR BER 7 H E 8 1 4 O Bu _ _ 4 4 O C MORBI 4 D O 1 10 u L 5 OX 1 5L A x1 2 20 gL 5 D O ED O BE
44. 8 8 RNA a DEPC RNase RNA 1 DNase RNase 2 DNase RNase a a
45. Sa LAMPI ER Hn T 4 hT H1S a e ra pr 4 AV ven dury ETTET da a 19UL urat u win 1 lt RA A MP 7 o LP La Tara nr ar ah cn act Od LAMPE HEN AS FE BIS M A ae 74 PEE DE R L T l IT IIT IV LAMP Belegen oc BAER H IIASO B4SFHH 777 BEOBBR 77 77 LAMP Loopamp LOPID Ta Ai oe rn ot rend ao LAMPIE HN DE EMTA MAA lt ER IE TE Z VE B L T AMP TP AKO See
46. e 1M Tris HCI 10 u L 2 000 x g 60 Total en 20 u L BEZ 2200 5 2 LAMP 20 5 u L 65 C 1 ERRSE 80 C 22H 1 LAMP Loopamp a IL CEM LAMP LoopampB 2 O Loopamp8Q 1 Extraction Solution for Foods EXF 1 8mL X 2tubes 48 2 1M Tris HCI pH7 0 Tris 3 2xReaction Mix RM 4 Primer Mix Cam PM Cam 5 Distilled Water DW 6 Bst DNA Polymerase Bst DNA Polymerase 7 Positive Control Cam PC Cam 0 1mL X Itube 3 1 Extraction Solution for Foods 2 1M Tris HC1 pH7 0 B E 3 2 X
47. a a 3 et EEE a E
48. 7 Wuenscher MD et al J Bacteriol 175 No 11 3491 3501 1993 8 Yoshino M et al American Society for Microbiology The 104th General Meeting 2004 9 25 p22 2004 10 54 No 3 667 715 1999 11 Aslanzadeh J Annals of Clinical Laboratory Science 34 No 4 389 396 2004 12 Prince AM Et al Biotechniques 12 No 3 358 360 1992 13 11275 98 103 2000 Let Don dee De 2m ar m Lo u al Jloopamp LAMPE IFAN CME Ta Miia M Ae E L ET
49. Reaction Mix 4 Primer Mix Cam 5 Distilled Water 6 Bst DNA Polymerase O A 8 5 Distilled Water A C amp 7 Positive Control Cam C 20C a 1 O K O a 1 0mL X1tube 0 6mL X1tube 0 12mL X 1tube 1 0mL X1tube 60uL X Itube 2 5 NT rd 2 N9 m TR gt dleopamf met lt s T a C gt io N re Ei da RR C A Distilled Water C Positive Control Cam
50. 0 EIFEL LT DA Mas EI IST LS FT FF 8 Berta D O 8 7 P 350 4A SQ e d S EVEN SET IV BET J Jb FERO AS u L 2 5 u L u L l1uL 2 x Reaction Mix 12 5uL x _ MI Primer Mix Cam Distilled Water Bst DNA Polymerase 10 LS Loopamp EE LAMP V LAMP LAMP D LAMP LAMPF 65 C60 m 80 2 20 SL
51. 20 u L 20 200 uL 200 1 000uL RE ARHKES1 I 15FYT7 20 u L 200 u L 1000 ug L 1 5mL 0 5 1 5mLH 8 1 5mL s n J Bee a maagi 73 I is F H er m m gt r xte 7id 80 3 7 MRI y ER 4 8 5 LAMP 3 Loopamp 5 DD a IIA Cjejuni C coli
52. A a 2 Reaction Mix non ARMS CRF Ast DNA Polymerase g A B COD RM przyne Nx irr Zo er BEL BD CBSA DEMIREL E a Bet DNA Pol merae RAI Per A B CD Enzyme Mix A M Distilled Water A D C B A N Primer Mix DNA RE B AI A DNA RNA UV FR HATE A DNA RNA
53. RNA DNA RNA UV E IDNase RNase DNase RNase DNase RNase
54. V 8 UV UV UV 1 UV UV UV 2
55. n a 10 25mL n LET a AP D a 5 BD a m IIA Cg co7 2 BSL2 10 P 28 E gt P 21 5 1 1 000L O 2
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