SYBR® Premix Ex Taq ™ （Tli RNaseH Plus）
Contents
1. DNA SYBR Green DNA DNA 1 CO Ltt tt ty tt pt S S D O E M 3 http www takara bio co jp 2 RR42LR ll 200 50 ul SYBR Premix Ex Taq 2 xX Tli RNaseH Plus ROX plus 5 ml X 1 x TaKaRa Ex Taq HS dNTP Mixture Mg2 Tli RNaseH SYBR Green ROX Ref erence Dye EGLO IV V 1 PCR authorized instruments 22. SN SYBR oar Taq 2 X Tli RNaseH Plus ROX plus 80 ee 30 en ners mae SYBR Green ROX Reference A TakaRa Ex Taq HS 7ag PCR
3. Smart Cyclere II System http www takara bio co jp 6 RR42LR VIII Appendix 1 PCR PCR SYBR Premix PCR RR8205S A B L W RR82LR WR RR091A B O No Template Control O
4. DNA template 100 ng RT PCR cDNA RT template PCR 10 2 Smart Cyclere Il Smart Cycler ll PCR PCR PCR Tm PCR 3 PCR PCR VIII 1 PCR Re Stage 3 Stage Optics Start End Optics DegiSec 9 i Of Degigecl Templ ecsloptiel BOO 950 Ch1 0 2 95 30 NA 95 0 5 Off Stage 2 PCR NA 60 0 20 lon Repeat 40 times 95 5 60 20 Stage 3 Melt Curve 3
5. eR i gt 95C 5 95C 5 95 C 5 95 C 5 2 oa T R 55C 30 55C 30 ie 72 C 30 72C 1 95 30 DNA 95 1 2 2 http www takara bio co jp 7 RR42LR SYBR Green Assay PCR 3 SYBR Premix Ex Taq Tli RNaseH Plus RR420S A B L W RR42LR WR SYBR Premix Ex Taq II Tli RNaseH Plus RR820S A B L W RR82LR
6. 3 4 PCR 120 126 2 2008 PCR 3 1 RT PCR 39 43 SYBR Molecular Probe Inc http wwwr takara bio coJp 11 RR42LR NOTICE TO
7. Ct PCR 100 0 1 uM 1 0 uM PCR O PCR EPS 95 C 5 95 C 5 60 30 64 C 30 O 3 step PCR PCR
8. 3 PCR 4 5 PCR VIII 2 RT PCR amp Perfect Real Time OCF http www takara bio cojp realtime 80 C 4 6 80 80 20 4 6 VI 1 2 3 4 5
9. BLAST 3 http wwwr takara bio coJp 8 RR42LR 1 OLIGO Primer Analysis Software Molecular Biology Insights 2 Primer3 http www genome wi mit edu ftp distribution software 3 http www ncbi nlm nih gov BLAST Perfect Real Time TEL 03 3271 8553 TEL 077 565 6969 3 RT PCR RT PCR PrimeScript RT reagent Kit Perfect Real Time RR037A B PrimeScript RT Master Mix Perfect Real Time RR036A B PrimeScript RT reagent Kit with gDNA Eraser Perfect Real Time RR047A B PrimeScript RT Master Mix Perfect Real Time RR036A B
10. PCR PCR 95 C 5 15 DOI eres PCR 95 C 30 http wwwr takara bio coJp 3 RR42LR Vil lt StepOnePlus Real Time PCR System gt Life Technologies 1 PCR 1 gt SYBR Premix Ex Taq 2X Tli RNaseH Plus ROX plus 10 ul 1x PCR Forward Primer 10 u M 0 4 ul 0 2 uM 1 PCR Reverse Primer 10 u M 0 4 ul 0 2 uM 1 template lt 100 ng 2 2 ul dH20 7 2 ul total 20 ul 1 0 2 uM 0 1 1 0 uM 2 template
11. gt 1 PCR 1 gt SYBR Premix Ex Taq 2X Tli RNaseH Plus ROX plus 12 5 ul 1x PCR Forward Primer 10 uM 0 5 ul 0 2 uM 1 PCR Reverse Primer 10 uM 0 5 ul 0 2 uM 1 template lt 100ng 2 2 ul dH2O 9 5 ul total 25 ul 3 1 0 2 UM 0 1 1 0 UM 2 template DNA template 100 ng RT PCR cDNA RT template PCR 10 3 25 ul 2 PCR PCR PCR Tm PCR
12. SYBRS Green RT PCR RT PCR PCR P 4 6 80 150 bp 300 bp 17 25 mer 40 60 45 55 Tm Forward primer Reverse primer Tm Tm OLIGO 1 63 68 C Primer3 2 60 65 C GC AT 3 T C polypyrimidine A G polypurine 3 3 GC AT 3 G C 3 T 3 base 3 2 base
13. DimerEraser Perfect Real Time RR091A B SYBR Premix Ex Taq GC Perfect Real Time RRO71A B PrimeScript RT reagent Kit Perfect Real Time RR037A B PrimeScript RT Master Mix Perfect Real Time RR036A B PrimeScript RT reagent Kit with gDNA Eraser Perfect Real Time RR047A B One Step SYBR PrimeScript PLUS RT PCR Kit Perfect Real Time RRO96A B One Step SYBR PrimeScript RT PCR Kit Perfect Real Time RRO66A B One Step SYBR PrimeScript RT PCR Kit II Perfect Real Time RRO86A B Thermal Cycler Dice Real Time System Single TP850 TP870 Thermal Cycler Dice Real Time System Lite TP700 TP760 Smart Cycler Il System SC200N SC210N Perfect Real Time http www takara bio co jp realtime RefSeq RT PCR SYBRS Green RT PCR 1 2005
14. 1 1 gt 5 X PrimeScript RT Master Mix Perfect Real Time 2 ul 1x total RNA x ul RNase Free dH2O up to 10 ul 10 500 ng total RNA CF 2 37 C 15 85 C 5P 4 3 PCR VII http wwwr takara bio coJp 9 RR42LR E StepOnePlus Real Time PCR System Mouse Heart total RNA 1 pg 100 ng PCR Mouse GAPDH Perfect Real Time Snara Curve namn lt ei http www takara bio coJp 10 RR42LR IX X XI SYBR Premix Ex Tag Tli RNaseH Plus RR420S A B L W WR SYBR Premix Ex Taq Tli RNaseH Plus RR820S A B L W LR WR SYBR Premix
15. 3 PCR PCR VIII 1 PCR ee FE Cosree nt Ophors j Hold nas ae r Cycle 1 ELY 95 C 30 C 2 Step PCR sunsa Cycle 40 95 5 60 C 30 Dissociation oe eee 3 Thermal Cycler Dice Real Time System http www takara bio co jp 5 RR42LR lt Smart Cyclere Il System gt 1 PCR 1 gt SYBR Premix Ex Taq 2X Tli RNaseH Plus ROX plus 12 5 ul 1 X PCR Forward Primer 10 u M 0 5 ul 0 2 uM 1 PCR Reverse Primer 10 u M 0 5 ul 0 2 uM 1 template lt 100 ng 2 2 ul dH20 9 5 ul total 25 ul 1 0 2 UM 0 1 1 0 uM 2 template
16. DNA template 100 ng RT PCR cDNA RT template PCR 10 2 PCR PCR PCR Tm PCR 3 PCR PCR VIII 1 PCR e mam Holding Stage Step1 95 C 30 Cycling Stage a Number of Cycles 40 Step1 95 C 5 Step2 60 C 30 Melt Curve Stage 3 StepOnePlus Real Time PCR System http www takara bio co jp 4 RR42LR lt Thermal Cycler Dice Real Time System Single SKU Lite
17. PURCHASER LIMITED LICENSE L11 SYBR Green I This product is covered by the claims of U S Patent No 5 436 134 and 5 658 751 and their foreign counterpart patent claims Takara PCR products containing SYBR Green are sold under license from Molecular Probes Inc only for the usage in Real time PCR for internal research purpose These products are not to be used for the purpose such as providing medical diagnostic or any other testing analysis or screening services or providing clinical information or clinical analysis in return for compensations L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries L46 SYBR Melting Curve Analysis The purchase of this product includes a limited non transferable license for all fields other than human or veterinary in vitro diagnostics under specific claims of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 owned by the University of Utah Research Foundation or Evotec Biosystems GmbH and licensed to Idaho Technology Inc and Roche Diagnostics GmbH to use only the enclosed amount of product according to the specified protocols No right is conveyed expressly by implication or by estoppel to use any instrument or system under any claim of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 other than for the amount of product contained herein L52 Rox Reference Dye Research Field Use of this product is covered by one o
18. WR SYBR Premix Dimer Eraser Perfect Real Time RR091A B SYBR Premix Ex Taq SYBR Premix Ex Taq ll SYBR Premix DimerEraser Tli RNaseH Plus Tli RNaseH Plus RR420 RR42LR RR820 RR82LR RR091 2 PCR RT PCR amp Perfect Real Time http wwwr takara bio coJp realtime RefSeq RT PCR
19. as RR42LR TakaRa SYBR Premix Ex Taq Tli RNaseH Plus ROX plus v201309Da SYBR Premix Ex Taq Tli RNaseH Plus ROX plus SYBR Green PCR 2 SYBR Green ROX Reference Dye RNaseH Tli RNaseH 2 x THEY cDNA mRNA PCR 7ag PCR FABER TaKaRa Ex Taq HS PCR ROX Reference Dye 2 StepOnePlus Real Time PCR System Life Technologies ROX Refer
20. ence Dye Thermal Cycler Dice Real Time System Single TP850 TP870 3 Thermal Cycler Dice Real Time System Lite TP700 TP760 Smart Cycler Il System 4 SC200N SC210N 1 Molecular Probes Inc SYBR Greenl SYBR Molecular Probes Inc 2 ROX Reference Dye II Applied Biosystems 7500 7500 Fast Real Time PCR System Life Technologies ROX Reference Dye SYBR Premix Ex Taq Tli RNaseH Plus Bulk RR420L W LET 3 Thermal Cycler Dice Real Time System TP900 TP960 SYBR Premix Ex Taq ll Tli RNaseH Plus RR820S AB L W RR82LR WR 4 Smart Cycler Cepheid 7aKaRa Ex Tag HS PCR PCR SYBRe Green PCR ABBR TaKaRa Ex Taq HS
21. r more of the following US patents and corresponding patent claims outside the US 5 928 907 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent claim and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA M40 Thermostable RNase H This product is covered by the claims of U S Patent No 7 422 888 and its foreign counterpart patent claims M57 LA Technology This product is covered by the claims 6 16 outside the U S corresponding to the expired U S Patent No 5 436 149 M82 Tli RNaseH Plus This product is the subject of the pending JP patent application TafdR4 Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp SII AA RNS AL v201309Da
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