“WAKO BIO WINDOW” No.137(2015.06)
Contents
1. 3 cGMP 21 CFR 210 211 1ISO9001 No AF 515 24921 211906 454q 8 100 298147 BD BBL Phytone Peptone 5lb 2 3kg 292450 2 OO 10kg 521 04725 212488 NB 500g 14 700 9 BD Difco Select Soytone O 10ko Saccharomyces cerev2. HeLa HepG2 MDCK COS 7 ES THP 1 RAW264 7 DNA siRNA ScreenFect A x1 Biomaterials 2012 Nov 33 32 8160 6 2012 2 Screenrect A Screen ect
3. a No 039 24155 CultureSure Sterile Water 500ml 2 500 No 032 23981 5 100g 15 000 D 6 3 8 CultureSure D Glucose Animal derived free 500g 40 000 No 037 24031 8 3 100g 15 000 7 039 24035 CultureSure Calcium Chloride Animal derived free 500g 40 000 032 24081 3 100g 15 000 M 034 24085 CultureSure Potassium Chloride Animal derived free 500g 40 000 030 24261 3 5 100g 15 000 5 7 032 24265 CultureSure Manganese I Chloride Tetrahydrate Animal derived free 500g 40 000 035 24191 8 100g 15 000 B 6
4. SDS PAGE DIRECT BLOT BM 80 5 HARP SDS PAGE SDS PAGE SDS PAGE Blotting PAGE
5. p http www siyaku com Ref 2 10 F 20 80 80 I 1 TT I HT MT W 2 2 6 384well plate Hela 100 200 400 800 1600 3200 cells well ATP CT RLU HeLa ATP HeLa CT 15 000 15 000 12 000 12 000 9 000 9 000 ad 6
6. 2 CEP CEPR1 CEPR2 CEP CEP CEPR NRT2 1
7. 1 2 10 10 300 000 ATP 250 000 P C _ 200 000 150 000 aa 100 000 50 000 0 ATP P C 3 ATP ATP ATP jz rn ro ATP 5 JATP HepG2 20k _ ATP HepG2 10k 90 000 ATP HepG2 5k ATP Hela 20k ATP Hela 10k ATP Hela 5k JATP
8. NH SH a NH bp SH 3 9 4 1 50 200ug a m a 50 000 Reducing Agent 5 000 1 OKI My ge T 37 C 10 1 4 To 6 SH reactive reagent Q OKI DS o
9. 83 2 14 17 2015 No 291 77401 5 1 000 65 000 ne New F Fluorospark Kinase ADP Multi Assay Kit 10 000 K W Pp htitp www wako chem co jp siyaku jutaku index htm 21 Products Native form 5 HT1A 5 HT gt c ce Y 5 HT 4A6 5 Hi m 4A6 Wako 5 HTx 2H 6D2 5 HT 5 HT gt c 5 HT G DNA
10. BM 80 DIRECT BLOT 1 998 000 BM 810012 DIRECT BLOT 10 SDS PAGE 10 30 000 M 0O htip www wako chem co jp siyaku jutaku index htm 31 HM Products pofinpo Dojindo Labeling Kits pomao nouzuin amas nc Dojindo Labeling Kits 3
11. 5000 cells medium with FCS EE mm P AN a Zz Gas Control Module O CO 5 200 38 1 000nm OD FRET DNA TR FRET ee amp BRET 1 2 3 lt 30
12. one pot assay 7 amp Z Lox 1 HepG2 S N No 623 01411 MS 9024X PrimeSurface Plate 24F 24 wells 3 4ml 1 10 12 000 631 21031 MS 9096U PrimeSurface 96U Plate U 300 1 20 30 000 627 01431 MS 9096W PrimeSurface 96U White Plate U 300 1 20 40 000 628 01101 MS 9096M PrimeSurface 96M Plate V 2004l 1 20 40 000 625 01091 MS 9096V PrimeSurface 96V Plate V 3004l 1 20 50 000 624 01441 MS 9384U PrimeSurface 384U Plate U 100l 1 20 50 000 621 01451 MS 9384W PrimeSurface 384UWhite Plate U 100l 1 20 62 500 630 28701 MS 9035X PrimeSurface Dish 35mm 5 50 9 000 637 28711 MS 9060X PrimeSurface Dish 60mm
13. 150 100 75 50 EY bt a T 25 n 4 20 SDS PAGE 15 er 10 09 10 Pe PVDF 2 BM 80 SDS PAGE BM 810012 mm 100x 6 6x 50 lt 1 oN 50 9 mm 80 mm W x50 mm H mm 245x 415 5x 170 oy A AC100V 50 60Hz 100VA 10 0 LCD 2 x 16 12 5 35 35 85 RH ESTEE 7 9 ls a AE i e
14. 2 1F BioWindow 6 No 137 3 8 lt II RW
15. Ref 2 10 F 20 80 1 1 TI on 1 1 2 1 2 ELISA 1 2 1 1u9 100ng 10ng 1u9 100ng 10ng 6 0 8 O 805 B a O 0 4 8 lt 0 2 2 BSA TMB 0 1 2 3 4 5 HRP Concentration of Biotinylated BSA ng ml B HRP 1 BSA ELTSA TMB OO 2 HRP
16. 0 2 iPS StemSure hPSC AF Wako StemSure hPSC AF iPS DMSO iPS iPS 1 iPS D PBS Accutase 2 10umol Y 27632 iPS hPSC ROCK
17. ga 5 aa 405nm 488nm 561nm 640nm e 5 sCMOS 2 560x2 160 16 6x14 0mm a XY 0 1 m XY 0 1pm Z 0 1pm 6 2x 4x 10x 20x 40x 20x 40x 10x 20x 16bit TIFF OME TIFF PNG JPEG WMV MP4 FCS CSV ICE 24 CO 79 6 O 4 gt
18. FP001 FP001 FCeM a FP001 FCeM D F FCeM MEM FCeM R FCeM D 4 ly 5 5 EGF TGFowv Epithelial Mesenchymal Transition EMT FP001 FCeM D
19. 6 384 6 2 HD 2CLR Ti HD High Definitton 2 CO gt m Cell able 96 DO ES 9 I a NN 8 Erlotinib 30 nM
20. HPLC 96 896 CseHazNO 1030 29 CAS No 162635 04 3 2 No 203 20131 EE 5mg a J IF Temsirolimus mixture of isomers 25mg _ m mTOR a No 033 21951 1mg 9 000 ES IF Compound 401 Sng 30 000 051 08771 a 5mg 15 000 057 08773 I EO a 25mg 52 000 Ge 115 00881 Ref KU0063794 5mg 25 000 165 24441 Rf PP242 5mg 25 000 184 02531 1mg 20 000 180 02533 IF Rapamycin mixture of isomers 10mg 54 000 188 02534 50mg 180 000 2 9 K C El 540 8605 1 2 TEL 06 6203 1788 5 103 0023 4 1 TEL 03 3270 8243 e TEL 092 622 1005 e TEL 082 285 6381 TEL 052 772 0788 TEL 0466 29 035
21. HRP HRP HRP 347 90911 LKO1 Ref Fluorescein Labeling Kit NH 3 21 600 348 91041 LK14 Ref HiLyte Fluor 555 Labeling Kit NH 3 21 600 345 91051 LK15 Ref HiLyte Fluor 647 Labeling Kit NH 3 21 600 346 91221 LK16 Ref HiLyte Fluor 750 Labeling Kit NH 3 49 000 341 91433 1 20 600 345 91431 LK31 Ref ICG Labeling Kit NH gt 3 46 400 349 90971 LK21 Ref Allophycocyanin Labeling Kit NH 3 44 200 PR 347 91011 LK23 Ref R Phycoerythrin Labeling Kit NH gt 3 44 200 346 90981 LK24 Ref Allophycocyanin Labeling Kit SH 3 42 000 344 91021 LK26 Ref R Phycoerythrin Labeling Kit SH 3 42 000 347 90891 LK03 Ref Biotin Labeling Kit NH 3 12 400 348 90941 LK10 Ref Biotin Labeling Kit SH 3 12 400 348 90821 LK11 Ref Peroxidase Labeling Kit NH 3 17 600 345 90831 LK09 Ref Peroxidase Labeling Kit SH 3 17 600 343 90871 LK12 Ref Alkaline Phosphatase Label
22. mPa s 1 1 LPaj 002 10 1 FCeM D HepG2 HepG2 FCeM FCeM A549 A375 HCT116 HeLa MCF7 MDA MB 231 7 HepG2 A549 A549 MNNG NOS 5 2 AGS MIAPaCa 2 A431 SKOV3 HB EGF HCT116549 HCT116549 2 FCeM A549 HCT116 FCeM FP001 Preparation kit KN B 8 bp hittp www siyaku com Ref 2 10C F 20 80 80 1 1
23. 2 CEP Ba CEP lI CEP LRR Leucine Rich Repeat CEPR CEP1 1 Annu Rev Plant Biol 65 385 2014 2 Plant J 55 152 2008 3 Science 346 343 2014 No 332 44871 7 000 G FJ htip www wako chem co jp siyaku jutaku index htm 35 Products gt Wako mTOR FK506 12 FKBP 12 mTOR
24. 0 oN 0 Ci ND _ a 300nM Ps 2 2 ee A A eve AOIIGD SS GO KS 2 HD 5 gt OR 8 a 3 PC9 SE CC 8 2 wr SN AA 1 3 6 9 43 8 0 sa CellPlayer 96 Well Caspase 3 7 Apoptosis Assay Kit 2 2 5 SM 0 10 20 30 40 50 Si 5 SC DS 69 A 2 nen ccm 3 05um pixel 4X 5 440 480nm 504 544nm 1 22um pixel 10X 565 605nm 625 705nm 0 61um pixel 20X 8 Terabyte 1 392x1 040 5 4 3A 100V 50 60Hz JPEG PNG TIFF RAW LE
25. RNA RNA RNA New OD PT Extraction Buffer ore eheee 30mlx1 RNA PT Binding Buffer oo 40mlx1 PT Wash1 Buffer tt or 40mlx1 PT Wash2 Buffer MM NE 2 dd Nevadaasaseae 40mlx1 DNase I DNase I RNase free eee 2 000 unitsx1 10xDNase I Buffer nn 1mlx1 ddWater RNase free ee 1mlx8 Spin Column eo 50 RNA 0 3 0 25
26. 8 2 2 2 a MM 2 25 280 000 21 500 000 17 150 000 1 2 2 M O htitp www wako chem co jp siyaku jutaku index htm 9 Products 7 9 Ba NII lI 7 10 _ ESSEN IncuCyte Z00M RSEN IncuCyte ZOOM CO gt 6 384 6 CO gt
27. 4D CO 0 9 a 1 FCS CSV ICE 3
28. Pe a a a so y y2 3 a 5 1 PBS 0 1 1 5ug cm 511 E8 a5 E8 1 y1 E8 5 8 2 3 511 o5 1 y1 3 511 E8 amp 681 0 5ug cm 1 05mg 175ugx6 6well plate 36 a 0 5 Non coat a OO 2 EC D iMatrix b iMatr
29. 030 24021 1mg 15 000 036 24023 IF CultureSure Y 27632 5mg 40 000 034 24024 25mg 150 000 199 16051 106units 30 000 F i 7 Te IF StemSure LIFE Mouse recombinant Solution ID 150 000 515 96451 2650 F_ SB590885 TOcris 10mg 63 000 K KA Pp http www siyaku com Ref 2 10 F 20 80 80C 1 TH HI HL HIT 1 2 2 M CultureSure CultureSure WW Wako CultureSure
30. 2 Spacer length Lo a i No A 347 05041 50mg 10 400 343 05043 GO005 Ref GMBS WM 4 Maleimidobutyryloxy succinimide 7 2 8 2 100mg 17 600 344 05051 50mg 10 400 340 05053 E018 Ref EMCS A 6 Maleimidocaproyloxy succinimide 9 8 10 8 100mg 17 600 342 06191 H257 Ref HMCS _ N 8 Maleimidocapryloxy succinimide 12 4 13 4 50mg 18 600 345 06201 K214 Ref KMUS 11 Maleimidoundecanoyloxy succinimide 16 4 17 4 50mg 18 600 347 06021 S025 Ref Sulfo GMBS N 4 Maleimidobutyryloxy sulfosuccinimide Sodium Salt NLS 2 50mg 32 600 Hetero Rsf Sulfo EMCS NH SH 4950 0 6 Maleimidocaproyloxy sulfosuccinimide Sodium Salt AA NY 00 8 Ref Sulfo HMCS EU VD N 8 Maleimidocapryloxy sulfosuccinimide Sodium Salt NH uo SO Ref Sulfo KMUS UA 0 11 Maleimidoundecanoyloxy sulfosuccinimide Sodium Salt 2 al 9 ao 349 09141 S330 IF Sulfo SMCC 8 6 9 6 50mg 27 600 345 08261 S291 Raf SPDP 5 5 100mg 41 200 346 09151 S359 IF Sulfo AC SPDP 12 6 13 6 50mg 66 000 348 09111 B574 Ref BS3 8 7 9 7 50mg 17 000 3
31. ES ES Monkey BMT 10 cell expressed human LIF D MEM L 10 FBS 0 2um ES 1 1 000 1ml 1L ES No 091 07131 IF IM 12 5mg 19 000 162 25291 5mg 17 000 a IF PDO325901 re Mg 234 02741 IF WH 4 023 5mg 32 000 257 00511 1mg 12 000 253 00513 5mg 36 000 251 00514 924582 25mg 140 000 257 00516 1g 014 23961 i 10 g 39 000 23963 IF Activin A Human recombinant Animal derived free 129 05601 1ml 25 000 O 4 7 5 3 IF LIF Human recombinant Culture Supernatant 2 130 000 No
32. pT ce CA i VOV _ ix AST YGT En ES y 720 2111 1711 6 es 8 65 089 i 0480 lesSsapan b No 382 06215 WWP001 IF Fetal Bovine Serum BVD Virus Free 2 500ml G YM htip www wako chem co jp siyaku jutaku index htm 17 Ba lI Products BD BBL Select APS LB BD BBL Selec
33. 2 BLA Ent IP DRN E TE x N SS 2 5 HT mRNA 5 HT 4A6 5 HTi mRNA BLA Ent IP DRN 22 bp itN WWW SIVaKU C0I Ref 2 10 F 20 80 80C T I T 1 1 2 2 3
34. ADP ADP cr 9mlx1 6 6 100ulx1 500ulx1 540nm eeereeeeeeen 400ulx1 te 10mlx1 2 ke 10mmol ATP eee 100ulx1 10mmol ADP ee 100ulx1 FIuOTOSDark Kinase ADP Multi Assay Kit ADP ADP ADP ADP ATP 2 Z factor S B Pp http www siyaku com Ref 210 F 20C 80 80 I 1 T I HT MT W 1
35. 2 EE2 FIuOresDbrite YG 16661 10 0 05um 10ml 77 100 16662 10 0 10um 10ml 52 500 09834 10 0 20um 10ml 52 500 24051 10 0 30um 10ml 52 500 24052 10 0 35um 10ml 52 500 24053 10 0 40um 10ml 52 500 15700 10 0 50um 10ml 52 500 07766 10 0 75um 10ml 52 500 Ref Fluoresbrite YG Carboxylate Microspheres 15702 10 1 00um 10ml 68 600 09719 10 1 50um 10ml 68 600 17687 5 1 75um 5ml 68 600 09847 5 2 00um 5ml 77 600 17147 5 3 00um 5ml 84 500 i 16592 5 4 50um 5ml 99 900 18141 2 6 00um 2ml 77 100 18142 2 10 00um 2ml 77 100 Fluoresbrite YO 19775 10 0 05um 10ml 77 100 18719 10 0 10um 10ml 52 500 19391 10 0 20um 10ml 52 500 18720 10 0 50um 10ml 52 500 18449 10 Ref Fluoresbrite YO Carboxylate Microspheres 1 00um 10
36. Native form 5 HT 5 HT gt c Native form 5 HT 5 HT DNA 5 HT 4A6 5 HT 6D2 NO 4A6 6D2 IgG2b k IgG2a k Es 1 100 gt 1 2 000 1 200 gt 1 10 000 i 1 100 1 1 000 1 100 1 1 000 1 5 HT 4A6 5 HT 6D2 10 12um pH7 0 10 10g ml over night 1 5 HT a 5 HT c mRNA
37. ATP ATP ATP ATP amp 1 ATP amp 1 1 10 T S 3 EE Hela NH 3T3 NH 3T3 CT 2 2 10 ATP
38. 28 10 https Wwww SumiDe CO jD Droduct S Dio Drirmesurface Droteo primesurface 96u index html 5 FU vs TPZ 5 FU s Na DNA RNA 5 FU 5 fluorouracil e DNA e DNA a 1 2 1 4 3 48 72Hrs 600 TPZ 200 400 1 Sb 300 4 O lt 200 s 100 0 EE a HeLa HeDpG2 HeLa TT 5 FU TPZ
39. R amp D Systems Bio Techne Wako BioWindow a NOVUS BIOLOGICALS ANTIBODIES FOR NEUROSCIENCE 2 i 9 J 4 3 2 S ANTIBODIES FOR JIc13NI HLe NOVUS BIOLOGICALS ANTIBODIES FOR CANCER RESEARCH gt FINOVUS INOVUS 3IOLOGICALS 3IOLOGICALS STEM CELLS Y Mu Novus BIQLOWCALS WNT AUTOPHAGY WNT SIGNALING PATHWA DS LIFE SCFNCF BRANDS RDsrsrws NOYUS TOCRIS proeinsimpie Pathways of Neurodegeneration NAYvus in Alzheimer s Disease OS APOPTOSIS wwwr novusbio com novus novusbio com htitp www wako chem co jp siyaku jutaku index htm PATHWAY PROFILING ANTIBODIES amp REAGENTS Apoptosis Necroptosis amp Aufophagy NECROP
40. M M M GFP 5 M F TR FRET 2 F M F F TR FRET amp Humidity Cassette 30 CO gt O gt
41. SC0 SI IF StemSure Freezing Medium 12 000 a 6 CultureSure DMSO BSA 1 1 37 2 2 3 3 4 4 ge 5 80 6 150 80 150 30 80C 24 100 100 2 80 80 60 60 40 gt 40 9 O 20 20 0 0 8 IEA 922 35 Ref C
42. 3 1 000rpm 3 4 hPSC ROCK 5 1 000rpm 3 6 2x10 cells ml 500ul 7 80 150 80 150 iPS 201B7 StemSure hPSC AF A 0 20 40 60 80 100 Cell viability 1 1 hPSC ROCK 2 1 000rpm 3 hPSC ROCK 3 2x10 cells 6cm dish StemSure hPSC AF A 0 0 0 5 1 0 1 5 Relative confluence A 1 0 2
43. 4 5 80 6 150 80 iPS StemSure hPSC AF P11 ES D3 StemSure 150 30 80 24 a A 30 30 80 1 2x1086cells 1ml S co lt 80 1 150 5 S 0 30 pe gt 8 30 80 24 8 3 gt 40 G gt gt 5 37 s 10 20 0 0 0 0 500cells well 6 10 12
44. http screenfect A ead ScreenFect http screenfect jp ScreenFect A Google ScreenFect 1 MCF7 GFP DNA DNA 100ng ScreenFect A plus reagent 0 1pl 1 1 1 1 5 1 2 1 3 B GFP GFP DIC MCF7 7 ET 2 K562 GFP DNA A B A A LL G o m OU OR Ll a 2 K562 No Transfection Reagent Dilution Buffer 293 77101 0 2ml 10ml 0 2ml 12 000 299 77103 Rg ScreenFect A plus 1ml 50ml 1ml 45 000 297 77104 1mlx5 50mlx5 1mlx5 180 000
45. Wako BioWindow E mail biowin wako chem co jp FAX 06 6233 3409 htip www wako chem co jp siyaku jutaku index htm 33 Products 1 SH a Hetero bifunctional Reagents b Homo bifunctional Reagents a Hetero bifunctional Reagents 1 NHS OSu SH FaD SH POD ALP bp Homo bifunctional Reagents 2 NHS
46. SOSIPN Plant RNA 0 2 A SOSPIN Plant RNA 3 015 0 1 0 05 0 220 230 240 250 260 270 280 290 300 310 320 nm 220 230 240 250 260 270 280 290 300 310 320 nm 1 RNA ISOSPIN Plant RNA RNA ISOSPIN ISOSPIN A PlantRNA M A Plant RNA M 1 g 19 Agarose S M RNA Ladder Code No 311 06261 0 5 pg 19 Agarose S M RNA Ladder Code No 311 06261 2 RNA RNA rme ET 310 08171 F Ref ISOSPIN Plant RNA 50 32 500 ISOSPIN No Se PRSRODN ERR Re S
47. A A 1 amp O m a 100 3 a 0 05 Ln 0 05 0 05 1 10 1 000ng ml Bovine AlIbumin ng ml No 2 No 631 04311 AKRAL 120 Ref Lbis Albumin Rat ELISA Kit 96 55 000 634 04301 AKRAL 121 Ref Lbis AlDumin Mouse ELISA Kit 96 55 000 2015 2016 ne SS 2015rJU ID i oe Apolipoprotein B 48 ELISA KIT IILJrI ELISA KIT ELISA KIT
48. No 293 3201 0 2ml 12 000 299 73203 Ref ScreenFect A 1ml 50 000 297 73204 1mlx5 200 000 293 75901 0 2ml 18 000 299875905 Ref ScreenFect mRNA 1ml 76 000 297 75904 1mlx5 320 000 299 75001 0 2ml 14 000 295 75003 Ref Screen ect siRNA 1ml 55 000 293 75004 1mlx5 240 000 K O0Z bp htth www siyaku com Ref 210 F 20C 80 80 1 I TL HI HOI 1 2 1 2 RNA ISOSPIN Plant RNA RNA
49. 2 1 2 factor Fluorospark Kinase 1umol IATP Z factor 0 79 10umol IATP Z factor 0 90 100umol IATP 7Z factor 0 95 5 Conversion ADP 0 05umoM 5S96 Conversion ADP 0 5 mol 080090090090000d 5 a 60000 eee YeeoeoS CV 1 5 2 CV 1 3 50000 eeeeoeoeeeoeoe Conversion ADP 5uimo S B 1 7 ii 7 CV 1 2 S B 3 0 40000 30000 0 Conversion ADP Opmol l 30000 S B 6 1 eeeeeeeeeeee C 2 1 30000 20000 20000 eeeeeeeeeeeeeee 0 Conversion ADP Oumol l 20000 10000 10000 CV 2 6 Cc 10000 deeeeeeeeeeeed 0 Conversion ADP Opmol l CV 1 1 0 0 0 A 1umol IATP Z factor 0 53 10umol IATP Z factor 0 86 100umol IATP Z factor 0 78 4000 35000 we 9 CO ADP 0 05jpumolfl 30000 e 9 rey ADP Sumol 300 3000 e 5 Conversion ADP 0 5umol e e ev 2o000 20000 a S B 5 8 2000 S B 14 5 10000 100 0 Conversion ADP Oumol 1000 eae eeu CV 19 93 0 Conversion ADP Oumol l c000 PSP ae ADP Ojimol l
50. 8 FCeM D ea iPS Re 3 Products np ATP TOYOIN GROUP ATP
51. NAc shell BLA DRN PFC lt 1 4 ES NN NN 1 Ge 10 awt NAc shell RM 12pm es ET a kk mm pH7 0 10 1ug ml over night 3 5 HTzc mRNA 5 HT 6D2 5 HT gt mRNA NAc shell BLA DRN No 016 25981 8W Anti Mouse 5 HT Receptor Rat Monoclonal Antibody 4A6 50ul 30 000 013 25991 F AntiMouse 5 HT Receptor Rat Monoclonal Antibody 6D2 50ul 30 000 No hi 050 08741 5mg
52. I TT HI HL 1 2 R2 siyaku com http www siyaku com 2015 7 28 30
53. gt 1 6 59 SDS PAGE de 125 Nr 93 72 1 2 1 4 1 8 1 16 53 42 SDS PAGE 10 0 PVDF 31 150 10 4 Ee kDa 1 DIRECT BLOT 4kDa 4kDa PAGE a SDS PAGE
54. 014 24441 50mg 20 000 li Ref Azasetron Hydrochloride 200mg 65 000 pp 5 HT 2 5 HT 073 06131 i 5mg 9 000 N Ip lt 2 amp 079 06133 Ref Granisetron Hydrochloride mi y56 000 pp 5 HT 132 13701 Ref 2 Methyl 5 hydroxytryptamine Hydrochloride 10mg 17 000 5 HT 511 31731 2491 10 Xaliproden hydrochloride Tocris 10mg 38 000 yp 5 HT 321 42341 5g 9 500 329 42342 5 Hydroxytryptamine Hydrochloride Serotonin hydrochloride 33 000 yp K SU htitp www wako chem co jp siyaku jutaku index htm 23 Prod
55. EL CultureSure Thiamin Hydrochloride Animal derived free 500g 40 000 030 24381 8 100g MM 032 24385 CultureSure Nicotinic Acid Animal derived free 500g 037 24411 0 100g 12 000 EE a 039 24415 EE CultureSure Calcium pantothenate Animal derived free 500g 40 000 032 24461 CultureSure Folic Acid Animal derived free 100g 40 000 039 24471 100g 1 jp No ic_247o CultureSure Riboflavin Animal derived free 250g I No 034 24421 a 100g 030 24423 CultureSure p Aminobenzoic acid Animal derived free 250g 036 24361 5 3 100g 25 000 7 Eee CultureSure Sodium Pyruvate Animal derived free 500g 70 000 CultureSure DMSO DMSO 0 2um ne TL 031 24051 CultureSureeDMSO DMSO 23 000 K Y
56. BVDV Bovine Viruses Diarrhea Virus FBS BVDV BVDV 1 ee Ss a _ Certificate of Analysis FBS Product code WWP 001 Lot Number 140828 10 i FT1 MD FBS 1 BVDVI Nose KS86 1 BVDVT KZ 91 2 BVD PCR a PP 2 RT PCR BVDV He sm ET 3 RT PCR BVDV 3 BVDV 5 CT _ EN BVDV Nose KS86 1
57. CONTENIS P 04 ATP P 06 PrimeSurface P 08 FCeM P 09 CQ1 P 10 IncuCyte ZOOM P 11 StemSure hPSC AF P 14 ES iPS P 15 CultureSure P 16 iMatrix 511 P 17 Selborne FBS P 17 FBS BVD 2 P 18 BD BDBBL MSelect APS MWLB P 19 BD BDDifco W amp BD BBL W P 20 Fluorospark Kinase ADP Multi Assay Kit P 22 5 HTi 4A6 5 HTzc 6D2 P 24 P 26 Polysciences Fluoresbrite
58. EB _ ES 96 e e e e MS 9096U 0 MS 9096M 6 MS 9096V 96 0 MS 9096W 96 PrimeSurface MS 9096V E 5 a 18 6 Ap PrimeSurface
59. 2 FBS 5ELBURNE 0 Selborne FBS PT Selborne BSE Selborne CGMP EDQM FDA 9CFR 554 04855 FBS 04 IF FBS Fetal Bovine Serum Triple 0 1 Micron Filtered AU Origine 500ml G K BVD BVDV
60. 450Wx470Dx315H mm 20kg WMV AVI 6 2 0 42 5 95 432Wx546Dx140H mm 17 2kg No IncuCyte Zoom 7 I ZOOM HD 2CLR 1 14 930 000 RC 633 24891 4473 ncuCyte OO 2C 2 I TM 2 A 636 24881 4472 IncuCyte ZOOM HD 12 230 000 ES 636 24901 4474 IncuCyte ZOOM Cell Migration Invasion Kit 1 1 792 000 634 24941 9600 0011 IncuCyte ZOOM Angiogenesis Software 1 1 086 000 637 24931 9600 0010 IncuCyte ZOOM NeuroTrack Software 1 1 086 000 1 ZOOM Ch i Es 9600 0015 A 1 427 000 Cell Migration Software Module M 0 bp htth www siyaku com Ref 2 10C F 20 80 80 1 1 TT 1 2 T
61. amp P 28 Screen ect M A Plus P 29 ISOSIPN Plant RNA P 30 TECAN Spark W10M P 31 DIRECT BLOT BM 80 P 32 Dojindo Labeling Kits P 35 CEP1 C Terminally Encoded Peptide 1 P 36 BD BD Bionutrients 2015 2016 Novus P 07 P 18 P 24 P 25 P 33 P 34 27 a 201577 23 24 201577 28 31 38 Ref 2 10 F 20C 80 80 1
62. bp itN WWW SIVaKU C0I Ref 210 F 20C 80 80 1 1 TH I 1 2 1 2 1 1 Kn 1 KC NNIec DAPI SSEA1 K 7 ES D3 4 ALP StemSure ES ES D3 ee e
63. Pp htitp www wako chem co jp siyaku jutaku index htm 15 Products Sh A LD ei New iMatrix 511 511 E8 iPS ES a m PWM i a a 5 a1 a2 a3 o4 G 62 59 ea m CHO S
64. TIA ELTSA KIT ELISA KIT ELISA KIT ELISA KIT Derf Derf Wako BioWindow E mail biowin Qwako chem co jp ELISA FAX 06 6233 3409 G K 24 bp itN WWW SIVaKU C0OI Ref 2 10C F 20 80 80 1 1 HI 1 2 1 2 NOVUS abiatechne brand Novus Biologicals
65. 10 100 40 000 634 28721 MS 9090X PrimeSurface Dish 90mm 10 50 37 500 Pp htip www wako chem co jp siyaku jutaku index htm sMMILON 2014 2015 S BIO Sn Mn e Wako BioWindow E mail biowin wako chem co jp FAX 06 6233 3409 G K Ba lI Products FP001 FCeM FCeM
66. 200 1 000nm ljvy KI 1nm a 230 900nm 280 900nm a 8 1nm 0 04 10 CO2 0 1 20 9 O 300 850 nm 8 1 384 NanoQuant plate CellChip MM 2 28 0 5 ml 1 ml No 2 442 500 Spark 10M 13 005 000 M O 30 bp hitth www siyaku com Ref 2 10C F 20 80 80 1 1 TT 1 2 HT T
67. cE N 5 37 C 30 S S S 7 N ma e l QO 32 bp tt WWW SIVaKU C0OI
68. Oct3 4 K 4 4 iPS 201B7 StemSure hPSC AF 80 2 4 1 2 5 HH 3 Oct3 4 Nanog BC2LCN 4 StemSure hPSC AF iPS Pp htitp www wako chem co jp siyaku jutaku index htm 11 lI Products iPS 201B7 BT Tubulin 5 StemSure hPSC AF 5 iPS 201B7 Tubulin oe SMA AFP Stemsure D ME
69. Y 27632 Lck Lymphocyte specific protein tyrosine kinase ROCK ES iPS Src es FT HPLC 98 0 2 NN 0 HPLC 98 0 DMSO a CAS No 837422 57 8 1 CH CszHseNs04 568 67 l CAS No 331752 47 7 uF a a r 2HCI HO C 4H N3O 2HCI H gt 0 338 27 A LTF TGF B E coli expressed human activin A 0 2um 0 01ng ug EDs 2 0ng ml gt 5x10 units mg MCP 11
70. 1277 1280 25 Clegg et g 1987 Cell Biology 105 2 937 947 13 Katz er g 1984 Nature 310 5977 498 500 26 Denk et g 1990 Science 248 4951 73 76 MES AN 000DUoaauo 7 55ml 1 200500g eTris am Oo EDAC FE 750mg S 50 7 CH O CH CH N oc N CH sN Scho ll CH a C OH CH CH N C N CH aN ScH 3 gt C O NH H p gt O COOH EDAC Active Intermediate Protein Protein Coupled Microparticles on Microparticles to Microparticle 24350 1 IF PolyLink Protein Coupling Kit for COOH Microsparticles 65 500 U TN Pp htip www wako chem co jp siyaku jutaku index htm 27 Products 7 i 7 NII lI 7 28 Screen ect A plus My Wako Screenrect Ap us
71. TL HOT 1 2 1 2 CEP1 C Terminally Encoded Peptide 1 w ME SES C terminally Encoded Peptide 1 CEP1 RG Asp Phe Arg Hyp Thr Asn Pro Gly Asn Ser Hyp Gly Val Gly His CEP1 C Terminally Encoded Peptide 1
72. bp htth www siyaku com Ref 210 F 20C 80 80 I TL HOT 1 2 1 2 Animal Free amp Antibiotic Free BD Difco amp BD BBL BD AF lt AF lt AF BD AF AF
73. MS 9096V ES KhES 1 9 000cells well GMEM KSR NEAA 2ME 20uM Y 27632 5 CO 37 ES a a c I fF 1 Self Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs NakanoT Ando S Takata N Kawada M Muguruma K Sekiguchi K Saito K Yonemura S Eiraku M Sasai Y Ce Stem Cell 10 6 771 785 2012 b http www siyaku com F 20C Ref 2 10 80 80 TI I HL 1 2 PrimeSurface 44
74. T I HI 1 2 1 2 2D 3D 4D CO1 ne Quantitative Image Cytometer YOKOGAWA CQ1 CQ1 3D
75. o_o CVs13 8 LCV 10 4 0 0 5 Conversion Rate 0 Conversion Rate ATP ADP 1 10 100umol 0 5 n 16 Z factor ATP Z factor EI 1 1 Z factor Z factor 1 3xSDi 3xSD Avu Av SD SD high control 5 low control Av Av high control low control 1 HTS 0 5 H 89 PKA 15000 S 5 10000 Ne cAMP dependent protein kinase PKA 5000 2 H 89 i 1C 43 7nM ICso 40nMD a 3 2 1 0 1 2 3 4 5 Log H89 nM 6 PKA Km
76. 000 6 000 3 000 3 000 day ATP Z factor 0 9831 0 8866 0 9349 CT Z factor 0 8793 0 8718 0 7649 5 384well plate 1 2 6 ATP ATP ATP Z factor 2 No 10mlx 1 304 15361 CA10 ATP 100 96well 9 500 300 15363 CA50 MI 500 96well 20 000 ATP 6 ATP 1 4 A1 308 1536 CA100 ATP 1 000 96well 37 000 50mlx20 304 15366 CA1000 ATP 10 00
77. 037 24195 CultureSure Zinc Chloride Animal derived free 500g 40 000 038 24181 8 3 100g 15 000 030 24185 CultureSure Magnesium Sulfate Animal derived free 500g 40 000 i 033 24251 100g 15 000 7 035 24255 CultureSure Cobalt I Chloride Hexahydrate Animal derived free 500g 40 000 038 24321 p 6 100g 15 000 6 030 24325 CultureSure Copper I Chloride Dihydrate Animal derived free 500g 40 000 036 24241 CultureSure Hexaammonium Heptamolybdate Tetrahydrate 100g 15 000 038 24245 Animal derived free 500g 40 000 033 24011 1 100g 15 000 7 035 24015 CultureSure Sodium Chloride Animal derived free 500g 40 000 032 24221 2 100g 15 000 P41 3 7 034 24225 CultureSure iron I Sulfate Heptahydrate Animal derived free 500g 40 000 ga No 035 24331 100g gE a ht a CultureSure myo Inositol Animal derived free 500g 032 24341 6 5 100g 15 000 7 1 CultureSure Pyridoxine Hydrochloride Animal derived free 500g 40 000 i 039 24351 8 8 100g 12 000
78. ATP 0 40nmol 3000 a 20 20umol 2500 10nmol 2000 5umol Km ATP 5 1500 2 5hmol LL gt 1000 1 25umol 8 1umol 8 500 1 W 0 625umol 0 1 0 I 0 10 20 30 40 0 10 20 SU Time min ATP uimol l PKA Kempetide ATP 2xRT 5 PKA Km ATP Km 3 15umol 1 2 1 Hidaka H Watanabe M and Kobayashi K Properties and use of H series compounds as protein kinase inhibitors Methods Enzymol 201 328 39 1991 2 Flockhart D A and Corbin J D Regulatory mechanisms in the control of protein kinases CRC Crit Rev Biochem 12 133 86 1982 3 Kumagai K Kojima H Okabe T and Nagano T Development of a highly sensitive high throughput assay for glycosyItransferase using enzyIme Coupled fluorescence detection Anal Biochem 447 146 155 2014 4 web http www ocdd u tokyo ac jD 5
79. DAP I Rhodamine PA Coumarin Alexa Fluor 532 Cascade Blue y Ll INTENSITY 450 500 550 600 WAVELENGTH nm WAVELENGTH nm YELLOW GREEN YG 441 486 YG FITC Pacific Blue Coumarin BRIGHT BLUE BB 360 nm 407 nm 430 YELLOW GREEN YG 441 nm 486 nm Alexa Fluor 488 YELLOW ORANGE YO 529 nm 546 nm es SYTOX Blue 300 400 500 600 SYIOX Green WAVELENGTH nm EGFP FluOresbrite BB 19773 10 0 05um 10ml 77 100 19774 10 0 10um 10ml 52 500 18339 10 0 50m 10ml 52 500 17458 10 1 00um 10ml 59 900 Ref Fluoresbrite BB Carboxylate Microspheres 17686 5 1 75um 5ml 68 600 5 18340 5 4 50um 5ml 99 900 19102 2 6 00um 2ml 68 600 19103 2 10 00um 2ml 77 100 bp itN WWW SIVaKU C0OI Ref 2 10 F 20 80 80C 1 1 HH 1
80. NIH 20k ATP NH 10k 80 000 ATP NH 5k 70 000 60 000 50 000 40 000 30 000 amp 20 000 10 000 0 0 2 000 4 000 6 000 g 000 10 000 12 000 4 CT 5 C HepG2 20k P C HepG2 10k P C HepG2 5k P C Hela 20k P C Hela 10k P C Hela 5k P C NIH 20k PP C NIH 10k 140 000 P C NIH 5k 160 000 120 000 100 000 80 000 THoH DDDoanno ri me 2 gar 40 000 et 20 000 0 0 2 000 4 000 6 000 8 000 10 000 12 000 96well plate 3 5 ATP
81. ee et 05 Classical LB Broth m Select APS LB Broth Time in Hours No 515 97235 292438 BD BBL Select APS LB Broth 500g 9 800 No eR Ot LB Broth Miller Luria Bertani i 523 04045 240320 500g 9 200 240210 ELISA 2kg 30 300 BD Bionutrients BD Bionutrients BD 7 BD Bionutrients Es e BD Bionutrients BBL Select APS MLB Difco Yeast Nitorogen Base 1 Wako BioWindow E mail biowin wako chem co jp FAX 06 6233 3409 G K 18
82. 0 96well G YM htip www wako chem co jp siyaku jutaku index htm 2 W Products 2 PrinneSurface PrimeSurface 96 V 96 U 96 U 384 U 384 U 24 35mm 60mm 90mm 96 ES
83. 1 TEL 029 858 2278 TEL 022 222 3072 TEL 011 27 1 0285 0120 052 099 0120 052 806 esWako Chemicals USA Inc Wako Chemicals GmbH Europe Office http www wakousa com http www wako chemicals de Head Office Richmond VA Tel 49 2131 311 0 a _ Tel 1 804 714 1920 DM Boston Sales Office MA E mail biowin wako chem co jp Tel 1 617 354 6772 URL http www wako chem co jp 36 15620 0 iTp
84. 14 500 a Ref Eplivanserin Hemifumarate 25mg 53 000 yp 5 HT 5 HT 221 02191 RR 3 8 5mg 18 500 plee IF Volinanserin 25mg 74 000 5 HT T 190 16961 200mg 9 000 196 16963 Sarpogrelate Hydrochloride mixture of isomers 1 y36 000 yp 5 HT A 118 00991 10mg 9 000 4 7 ZOOGG Ref Ketanserin Tartrate 50mg 27 000 5 HT 5 HT 5 HT in VO 018 24841 i 5mg 8 500 IF Agomelatine 25mg 34 000 yp MT1 MT2 5 HT
85. 42 09131 D630 Ref DTSSP 8 7 9 7 50mg 18 000 6 345 09121 D629 Ref DSP 8 7 9 7 1g 30 400 NH NH 347 08601 D539 Ref Dithiobis succinimidyl hexanoate 16 6 17 6 10mg 14 800 344 08591 D538 Ref Dithiobis succinimidyl octanoate 21 8 22 8 10mg 14 800 347 08581 10mg 14 800 343 08583 D537 Ref Dithiobis succinimidyl undecanoate 29 7 30 7 50mq 43 400 Wako BioWindow e HPLC _ Wako BioWindow i 5 E mail biowin wako chem co jp FAX 06 6233 3409 G K 34 pit WWW SIVaKU C0I Ref 210 F 20C 80 80 I
86. Kinase ADP Multi Assay Kit Wako ADP Fluorospark Kinase ADP Multi Assay Kit High throughput screening HTS ADP ATP CoA ADP Z factor amp
87. M SSR 2mol L Glutamine 10mmol 2 Mercaptoethanol 1xNon essential Amino Acids Solution iPS 201B7 197 17831 Rf StemSure hPSC Freezing Medium AF 16 000 10ml No 5 257 00511 1mg 12 000 253 00513 5mg 36 000 251 00514 EA JGAa 25mg 140 000 257 00516 1g StemSure ES iPS maDMSO BSA ES gt 6 1 37 i 2 NR 3 4
88. N MEG 2 HPS es a 2 MHRGK SOLBEMa2oeo 2 a ey 8 M NA Pp http www wako chem co jp siyaku jutaku index htm 29 7 x W lI Products 1 0 SDark 10M SparkiM10M 1 SparkControl Fusion Optics amp amp Gas Control Module m 9 Fusion Optics F
89. TOSIS AUTOPHAGY U TN 25 Products AV p pd Polssiences Inc _ FIuoresbrite WW Polysciences Fluoresbrite DNA 3 8 2 2 26 m mm 1 2 5 YG 1 0um BRIGHT BLUE BB 360 407 YELLOW ORANGE YO 529 546 BB YO
90. Wako a DIO MI VS June 2015 http www wako chem co jp P03 P 04 ATP P 06 PrimeSurface P 08 FCeM P 09 CO1 P 10 IncuCyteTM Z00M Wi P11 P 11 Sten Sure hPSC AF y P 14 ES iPS W w FN SS 1 _ P 15 CultureSure 1 ET vw P 16 IMatrix 511 a 5 SNeie SN P 17 Selborne FBS Si Te SN PY et 17 FBS BVD 4 4 PN8 BD BD BBLTM Select APSTM LB F Lo LN ms ai P19 BD BP NM i a a N W li W ee 4 20 gt R
91. a I N P s s bh 5 1 0 I LT uorospark Kinase ADPIMulti Assay Kit Wi a a 22 S HT1A 4A6 NL a 5 HT2Q 6D2 I LE E24 a ML Pi a a W 1 1 ad 5 2 UL 9 26 BE NT P 26 Pace ea Fluoresbrite gm P 20 nw y i 1 lt gt P28 Sereeh Fect A Plus p29 AN 1 p30 8 1 lt 3 WW 8 I SC Wt A UEC SparMW 10MW al AL ua 2 Pa1 PR Me DIRECTBL OT BM 80s a PE ee i st i 1 nM i Rt es P32 Dk Mabeling Ki ML I OM 2 32 Doji abeling Kits at i 1 WA NN 2 PT 1 1 TY NK 5 lS EE 9 P39 4 P 35 3 CEP1 4 minal Encoder Peptide 1 P36 NN lt 1 GE A 7 Wako
92. ing Kit NH 3 21 600 346 90861 LK13 Ref Alkaline Phosphatase Labeling Kit SH 3 21 600 Wako BioWindow hain Labeling Kits Labeling Kits Biotin Labeling Kits Peroxidase Labeling Kits Alkaline Phosphatase Labeling Kits i ec amp Aa ioG e a Labeling Kits httpewww dollndo coJjp
93. isiae No AF 527 00305 212750 500g 18 200 525 00301 212720 BD Bacto Yeast Extract 0 2kg 69 200 523 00307 212730 10kg 291 200 521 01001 255772 a O 100g 8 500 255771 BD Bacto TC Yeastolate 9 10kg UF Ultra Filtered UF Ultra Filtered MWCO 10kDa 500EU mg No AF Need NN SS 0 5 i OS BD Difco Yeast Extract UF 0 208 0 BD Difco TC Yeastolate UF i AF AF G K Pp htitp www wako chem co jp siyaku jutaku index htm 19 Ba Products 20 ey Fluorospark
94. ix No 385 07361 NBi 892011 175 gx2 28 000 2 381 07363 892012 Ref iMatrix 511 solution 0 5mg ml 175ugx6 2 000 389 07364 892013 175ug 1 Google 16 bp itN WWW SIVaKU C0I Ref 2 10 C F 20 80 80 1 1 T I HI 1 2 1
95. ml 59 900 19392 5 1 75 m 5ml 68 600 19393 5 3 00um 5ml 84 500 19394 5 4 50um 5ml 99 900 19395 2 6 00um 2ml 77 100 1 Sanders et al 1989 Limnol Ocegnog 34 4 673 687 14 Quattrochi et al 1987 Exp Neurology 96 1 219 224 2 Wolf et al 1987 Nucleic Acid Res 15 7 2911 2926 15 Koerten et al 1980 Exp Cell Res 128 2 470 475 3 Kremsky et al 1987 Nucleic Acid Res 15 7 2891 2908 16 Cornwall et al 1988 J Neurosci Methods 24 1 1 9 Ti 4 Corash et al 1989 Exp Hematol 17 3 278 286 17 Wells et al 1988 Infect Immun 56 1 278 282 5 Blume P 1989 Cytometry 10 3 351 353 18 Callebaut et al 1989 Stain Technology 64 2 100 102 6 de Grooth et al 1987 Cytometry 8 6 539 544 19 Pierce et al 1989 Stain Technology 64 5 249 251 7 Stewart et al 1986 Methods in Enzy 132 183 192 20 Herzog et al 1979 Eur J Cell Biology 19 9 203 215 8 Oda et al 1986 J Immunol Methods 88 2 175 183 21 Hampton et al 1987 Anat Rec 219 4 338 346 9 Stewart et al 1986 Methods in Enzy 132 183 192 22 Hale et al 1986 Amer J Physiol 251 4 Pt 2 863 868 10 Steinkamp et al 1982 Science 215 4528 64 66 23 Harvey et al 1989 Environ Sci Technol 23 1 51 56 11 Harmsen er al 1987 J of Luek Bio 41 2 95 103 24 Harmsen er al 1985 Science 230 4731 1277 1280 12 Harmsen et al 1985 Science 230 4731
96. t APS M LB Escherichia coli BSE 1L BSE TSE MK NN 2 5g eu 12 5g ER TD ARR RAR EE 5 0g IM 5 E coli DH5q a I LB 5 E 3 Select APS LB E coli sl 0 DH5a Select APS LB 0 co 5 2
97. ucts ELISA ELISA BSA 0 78v50ng ml 2 30 100l well 5 4 96 1 C V 10 500ng mD ee 200ulx1 3 3 4 u 60mlx1 C V 10 ee 200ulx1 WM oR 12mlx1 1M H2SO4 ee 12mlx1 3 10x tee 100mlx1 Re IN RAR NN RR NI 3
98. ultureSure Freezing Medium 100ml 8 000 K Y Pp htip www wako chem co jp siyaku jutaku index htm 13 Products 9 Ba I FTHHHT 14 ES iPS ES iPS ww Wako IM 12 PD0325901 SB590885 WH 4 023 Y 27632 Activin A hLIF ES ES PD0325901 1uM IM 12 1uM SB590885 0 5 M hLIF 20ng ml _ WH 4 023 1uM Y 27632 10 M Activin A 20ng ml Theunissen T W et al Ce Stem Cell 15 4 471 2014 TM 12 PD0325901 GSK 38 glycogen synthase kinase 38 MAPK oH HPLC 98 0 HPLC 98 0 9 9H Os O lt SN DMSO DMSO a CAS No 112 29669 05 1 CAS No 391210 10 9 C gt gt HzoFN30 gt 377 41 N Ch N C H 4F3IN gt 0 482 19 VH 4 023 aa
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