DNA/RNA調製法 実験ガイド
Contents
1. RNA RNase RNase OFF 9037 RNA RNA DNA RNA 1209 RNA RNA RNA RNA ma RN2. PCR DNA PCR 1 A NN 12 000 15 000 rpm 3 B 100 500z1 TE 10 mM Tris HC1 0 1 mM EDTA pH8 0 C DNA RNA 1209 2 Chelex A 200 1 10 Chelex B 95C 10 C Vortex D 12 000 15 000 rpm 1 DNA Chelex 100 Resin Bio Rad 142 1253 10 Chelex
3. 3 A 100 1 250 U ml in TE buffer B 387 55C 10 15 C 100 1 10 Chelex D 95C 10 E Vortex E 12 000 15 000 rpm 1 DNA 014 09661 DNA 026 O111 0157 0515 8 0 1 ml 10 000xXg 10 50 mM NaOH 85 1 100C 10
4. DNA DNA DNA 100 500z1 TE 10 mM Tris HC1 0 1 mM EDTA pH8 0 95 C 10 12 000 15 000 rpm 1 DNA 501 DNA RNA 1209 100 mM NaOH 50x 1 95C 10 1M Tris HC1 pH7 0 11 1 12 000 15 000 rpm 1 DNA OO SimplePrep reagent for DNA
5. 1M Tris HC1 pH7 0 15 1 2 000 10 000Xg 10 DNA DNA RNA 1209 4 DNA DNA DNA NucleoSpin Tissue DNA 740952 50 NucleoSpin Tissue XS DNA 740901 50 NucleoSpin Soil DNA PCR 740780 50 5 RNA RNA RNA DNA RNase
6. 9180 100z1 0 2 ml PCR SimplePrep reagent for DNA Reagent A 201 Reagent B 41 20 1 387 C 6 95C 8 80x 1 DNA 3 DNA 1 3 ml 1 000 rpm 1 1 0 1 2 ml 12 000 15 000 rpm 3 100 500 1 TE 10 mM Tris HC1 0 1 mM EDTA pH8 0 95C 10 12 000 15 000 rpm 1 DNA PCR
7. A II total RNA 740955 50 NucleoSpin RNA XS total RNA 740902 50 NucleoSpin RNAY Plant total RNA 740949 50 DNA RNA 1209
8. DNA RNA PCR DNA RNA RNA DNA DNA DNA RNA O1 OO MN DNA RNA 1209 1 20 200 1000 1 15ml Vortex 1 5 ml 2 DNA
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