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1. lt 0 99 5 50 75 100 120 135 V CE UL 5 50V 75V 100V 120V 135V 0 99 gt 1 4 9 MV ap 1 1 A i MyRun N MM 1 WP 7 2 a2. ChIP Chromatrap gt mm 3 N gt a C owa G NE DNA 25 2 lt 3 100 ng 3000 ng IP 7 500 ChIP ChIP ChIP Chromatrap ChIP Chromatrap ChIP Chromatrap
3. DFKZ Material Transfer Agreement MTA Web http www cosmobio coJp supportdocument0105 CLI B pdf Web ERE ID RNA Small RNAs MOR F Sc Molecular Research Center Inc RNA RNAzol RT 7 N es RNAzoIl RT DNA RNA mRNA smallRNA RNA 00 bases 10 40 base miRNA large RNA ol mm EE DNA Ready tO uS OM IM e 1 J RT PCRH qRT PCRH
4. 124x120mm 124 W X120 Dmm 07 Wx60 Dmm 52 Wx60 Dmm _ 26 156 6 RT 5 13 78 6 50 48 8 12 2 2 4 13 18uL 17 Xx2 8 4 MyRun 1 t 1 MR2Z 001 1 unit 35 00U EE 6 Eg 130 4 2015 2
5. 24 ChIP 24 PCR Premium Chromatrap Pro A ChIP Spin Column kit 500115 24 assay 95 000 Prermium Chromatrap Pro G ChIP Spin Column kit 500116 24 assay 95 000 Chromatrap ChIP Chromatrap A G 24 ChIP 24 Standard Chromatrap Pro A ChIP Spin Column kit 500071 24 assay 88 000 Standard Chromatrap Pro G ChIP Spin Column kit 500117 24assay 88 000 96 Chromatrap A G 96 96 ChIP ES Standard C
6. Bioruptor Bioruptor UCD 300 UCD 250HSA UCD 250 UCW 310 a es 200 250 300 W 160 200 250 W 6 TOS 130 200 250 310 W 1 60 0 99 59 1 60 ON 1 99 0 99 99 1 99 OFF 30 99 0 99 99 30 99 0 5Im 6 6 1 5 10 15 mL or 3 50mL 0 5 mL 1 5mL UCW FY A 2 Bioruptor Bioruptor UCD 300
7. ID WWww cosmobio co jp 8 SA 8B gal A SA 8 gal 3 SA gal SA B gal SA B gal 96 Well Cellular Senescence Cellular Senescence Flow Cellular Senescence Staining 2 Activity Assay Cytometry Assay Kit e96 e e e SA gal e pj gal e eSA B gal e 4 eSA B
8. RD NN PP ID Web Y dd i BH A 2 AE6 gal 1 7430 CLS Haca EN BNAGSERDpeso Ee ess MyRunjl 1 El 13671 0 Bs cforoxwo ED 2980 2995 9194 ID lt Gol 000 9 ID
9. a 2199 135 0016 2 2 20 URL http www cosmobio co jp TEL 03 5632 9610 FAX 03 5632 9619 TEL 03 5632 9620
10. poly A RNaese DNase RNA 1 ml RNAZoI RT Reagent 100 mg 50 700 ug large RNA gt 200 bases RNAzol RT Reagent 15 000 8 120 ug small RNA 10 200 bases 6 000 48 000 119 000 Web EER ID 62 i MyRun ll Mn 6 1 eg UV LED ww 156 124 x 120 mm 8 12 L S
11. Bioruptor UCD 250 Bioruptor UCD 250 Bioruptor UCW 310 05 1 5 10 15 50 mL MAT 310 05 MAT 310 15 UCD 300 UCD 250HSA UCD 250 UCW 310 24 X2 0 5 mL or 12 X21 15 10 15 ml or 6 X2 50mL BRAN 0 5 mL 1 5mL NG 310 TOS 1 350 000 1 080 000 900 000 1 800 000 T unit unit 0 5 1 5 mL TOS OS S 10S ml MN GC 1 9 000 NG 310 1 18 000 0 5 mL MAT 05 1 set 12 91 000 MAT 310 05 1set 24 170 000 US
12. DAI 1 Sy EE 9194 FITC Rhodamin Red X MM ww TEXAS RED ALEXA FLUOR DyLight gt _ Cyanine PE DAPI w 1 OK Hoechst33358 Hoechst3334 Propidium lodide ww 4C PEXA FLUORE TEXAS REDS OSClSL PBS Pife Technolo9ies Rhodamine Red DNV Immunoselect Antifading Mounting Medium SCR 3844 7 13 000 Immunoselect Antifading Mounting Medium DAPFI SCR 38448 17 000 Immunoselect Antifading Mounting Medium HI SCR 38449 17 000 ID 2980 015 1
13. qal eX gal e 5X E EE 13 iPS ES EC R 10G iPS ES TRA 1 60 IgM TRA 1 81 IgM EC iPS ES 5D4 BCD4 Nomarski Il TO PRO3 Il R 10G VTRA 1 81 X100 CAC kd X N mAb84 RA1 60 a Thr a 4 a 35 334 4tci V Merge VI
14. 129 133 2013 Anti Keratan Sulfate R 10G RIT MOO 1 el ee 40 000 Mouse R 10G EEE 13153 4 2015 2 3 HaCaT nx CeliLines Service CU SV40 1 oy UV _ HaCaT Human Skin Keratinocyte 300493 SF 1 vial 2x106Cells Vial 304 000 MG 30 DMEM with 4 5 g glucose ml 4 L glutamine and without FBS 8 0U300A S00 S00 1 10 FBS DFKZ
15. Close up view i Fe 1 5 a 1 R 10G jiPS Tic R 10G TRA 1 81 IV V V X400 bars 200 hm VI V TO PRO Life Technologies R 106 IPS ES R 10G iPS Tic SSEA 3 EF ils 4 8 3GalNAc 1 3Gal a 1 4Gal SSEA 4 EC 2102Ep NeuAco2 3Gal 1 3GalNAc TRA 1 60 EC 2102Ep TRA 1 81 EC 2102Ep iPS induced pluriopotent stemcells ES embryonic stemcells EC embryonal carcinoma cells TRA 1 60 TRA 1 81 2 1 Kwabe et al Glycobiology 2013 Mar 23 3 322 36 2 Schopperle et al WM Stem Cells 2007 Mar 25 3 723 30 3 30 10
16. M MAI 15 1 set 6 1 000 MAT 310 15 1set 12 140 000 UCD 300 Power M 30 ON 30 OFF 1 HeLa 1 5 mL 1x 10 n1 mL 15 mL ww 4 8 12 16 4 8 12 16 4 8 12 16 100 bp ladder 1 500 el 1 000 pi 500 pr 100 pa 1 10 11 x fixation solution formaldehyde 1 5 1 10 1 5 M glycine 2 000 rpm 5 4 FACS solution 10 4 2 000 rp
17. hromatrap Pro A high throughput ChIP 96 well Microplate 500161 kit 216 000 Standard Chromatrap Pro G high throughput ChIP 96 well Microplate 500163 kit 216 000 EE 12084 4 2015 2 7 BB DameyaembN ID DAI ai PAP S 4 600 i fer ID Web
18. m 5 4 n1 1 mL Cell lysis buffer on ice 10 1000 g 5 4 n1 200 nL SDS lysis buffer Protease inhibitor 200 pL 1 5 mL 10 Bioruputor 4 DNA 2 GelRed 30 ID AY Il llltelite aWelke 9 9 Chromatrap ChIP Porvair Sciences Ltd PVS 3 A G
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