B1-FITC
Contents
1. gt PBS PBS 2 8 C NBS 2 BSA 0 19 1 0 19 B1 FITC gt B1 FITC 1 500rL 100 000 xg 10 gt 1 5 L B1 FITC 5 L 195gL PBS2. 7 Fc 10 15 1mg mL 8 9 10
3. 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 0 196 1 2 1 6603152 300 PBS 24mL 1mL 9 2596 2 1L 8 26g 1 0g EDTA4Na 2Na 37m9 pH 7 2 7 4 1 1 2
4. Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3 Pan T Pan B T B pan B CD20
5. B1 CD20 488nm FITC FS 907 SS 1 EDTA 6
6. 3 96CV 79 1 0 196 3 4 5 6
7. MSlgG2a FITC 200L 4 45 5 a b a 2mL 5 15 b PBS 2 3mL 400 450 x g 5 PBS 25 1mL 30 2 250L 6 PBS 2mL 7 400 450 x g 5 8 6 7 9 0 5 1mL
8. 16 100 859 6 gt PBS PBS PBS 2 lt 8 NBS 2 BSA 0 19 0 19 PBS PBS 0 1 2 lt 8 gt 1 1 2 100L 3 200L
9. PBS 20 25 C 6 a gt 10 x 10 mm 10 20 x103 2 20 30 x10 3 30 40 x10 5 40 60 x10 6 60 100 x103 10 100 200 x10 20 b lt 3 x 10 mm 1 25 C 500 x g 5 2 3 4 LH 700 5 10 x 10 mm 1 100L 2 Ficoll Paque 1
10. 2 90 3 859 4 Ficol Paque Ficoll Paque 5 5 Ficoll Paque 6
11. IN 1 2 8 C 2 B1 FITC 2 8 6 0 20 6 70C 12 20 25 C B1 FITC ww BI1 FITC 6602381 100 0 5mL Stashenko _ PNadler LM Hardy R and Schlossman SF 1980 Characterization of a huma
12. 30 2 10 15 20 30 3 MslgG2a FITC B1 FITC 6602906 100 0 5mL 4 Propidium lodide Calbiochem 53705 0 01mg mL 0 05mg mL 5 Acridine orange Baker A366 3 0 005mg mL 6 Ficoll Paque Pharmacia 17 0840 03 EDTA 1 1 100 L 3 10 x 10 mm 10 x 10 mm3 3 x1097mm
13. CD20 B Fc B1 H299 CD20 B BALB cJ P3 NS1 AG4 Id lgG2aH zL B1 FITC FITC Fluorescein lsothiocyanate FITC 5 10 468 509nm 504 541nm 1 0 5mL B1 FITC 0 29 0 01M 0 15M 0 19 B B B1 CD20 B
14. 11 12 B B lgG 13 B1 FITC B CD20 14 15 16
15. 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 C 400 lt 450 xg 30 4 Ficol Paque 2 8 C PBS 2 8 C 400450 xg 8 6 PBS 7 2 lt 8C 400 lt 450 x g 4 8 PBS 9 2 8 C 400 450xg 3 10 PBS 1x 10 mm 1 x 10 mL 11 a b 9096 5
16. 9 EPICS Cytomics 3 Ficoll Paque 1 1 x10 200L Ficoll Paque U 96 200L 2 2 8 C 400 450 x g 5 3 4 5 200L MSlgG2a FITC
17. 2007 2 6602381 1999 2 20200EZY00025000 877429 B B1 FITC IN ww FITC Fluorescein lsothicyanate 1 2 5 g B1 CD20 30 35kD CD20 B T
18. B1 B T B B1 CD20 B B1 CD20 B1 B Pokeweed mitogen PWM B1
19. PBS 10 EPICS Cytomics 2 Ficoll Paque 1 12mm x75mm 2 Ficoll Paque 1 x 10 1 x 10 mm 1nL 3 2 8 C 400 lt 450 x g 4 4 200wL MSlgG2a FITC 200L 5 2 8 C 30 6 PBS 1mL 2 8 C 400 450 x g 4 7 5 2 8 0 5 1mL PBS
20. CD20 MSIgGAn FITC BI FITC CYTO TROL 6604248 MSlgG2a FITC 29 29 T T
21. 200L 6 2 8 C 30 7 2 8 C 400 450 x g 5 8 3 4 9 PBS 200L 2 8 C 400 lt 450 xg 5 10 3 4 11 PBS 200L EPICS Cytomics 1 2 Ficoll Paque 3 FITC Log 1
22. a 12 1 x 10 1 x 10 mm 1mL 13 2 8 C PBS 2 8 C 400 450 x g 4 14 0 05mg mL Propidium lodide 3 1 15 PBS 2 8 C 400 450 xg 4 16 15 17 85 b 12 25 000 1 x 10 L 25L 13 Propidium lodide 0 01mg mL 10L 14 30 Acridine orange 0 005mg mL 10L 3 15
23. n B lymphocyte specific antigen J Immunol 125 1678 1680 Reinherz EL Haynes BF NadleOr LM and Bemstein ID eds 1986 Leukocyte Typing II New York Springer Verlag Vol 2 p 8 20 Morimoto C Reinherz EL Nadier LM Distaso JA Steinberg AD and Shlossman SF 1982 Comparison of T and B cell markers in patients with Sjogren s syndrome and systemic lupus erythematosus Clin Immunol Immunopathol 22 270 Koepke JA and Landay AL 1989 Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 52 19 27 IO CO 135 0063 5 7 COULTER COULTER CLONE CYTO STAT EPICS FLOW COUNT Q Prep Multi Q Prep TQ Prep XL Beckman Coulter Inc 135 0063 5 7 Wa COULTER TCY036 07 09
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