L麦g2 主退り“ “麦g“
Contents
1. Ligation high 2 1 e 20C Ligation high 5 10 DNA e DNA Ligation high e 16C 30 e 2 DNA e2. 0 100 200 300 TE buffer NaCI mM 5 e DNA buffer TE buffer DNA 1 117X 109cfu pg pBluescript Il 7 5 Code No LGA 111 DNA 010 Competent high E coli JM109 DNA 900 pUC18 DNA PUC018 T4 DNA Ligase A Hind Ill digest 8 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience bio toyobo cojp 103 8530 17 9 TEL 03 3660 4819 FAX 03 3660 4951 E mail order_lifescience bio toyobo cojp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E
3. 3 pfuhg A ZAP II Ligation high Ligation high T4 DNA ligase 1 1 10 5 16 C x10min 220x10 7 68x10 16 x30min 520x10 8 32x10 16 yeomn 300x10 115x107 16 x16hr 3 21x108 9 35x108 2 15x105 26 x10min 4 80x105 512x10 26 x30min 120x10 320x10 26 x60min 200x10 3 84x10 26 x16hr 119x10 3 56x108 3 09x105 e 16C 26 e 30 5 4 1 nd 500rg 5l 6Gl 2 5u Ligation high 16 e DNA 1 es T4 DNA Ligase 2 Ligation high Ligation high T4 Ligase 1 10 5 C123456CIC123456CIC123456C Control 16 x 5min 4 16C x
4. DNA Liga tion high 2 DNA TE buffer DNA 1 10mM Tris HClpH8 0 1mM EDIA TD 3 Ligation high 1 T4 DNA Ligase 50 2 Ligation high DNA cfulg 10 2 1 0 5 11 12 1 3 T4DNALigase DNA Ligation high 1 DNA Ligation high 3
5. 10 Ligation high 1 1 Ligation high 1 0 5 107 a T4 DNA Ligase 10 5 10 0 100 200 300 400 500 3 2 Ligation high Ligation high T4 DNA Ligase 11 10 5 10 51 57 23 50 54 65 24 100 57 64 38 500 54 76 38 100 1 07 109cfu gg pBluescript 1 4 3 AZAP Il EcoR arm 250ng Test Insert 200ng DNA 5 e DNA 5ul 2 5ul Ligation high 16 26 10 1 e 4 GIGAPACK I Gold E coli XL1 Blue 1T4 DNA Ligase 16hr 2
6. 50 100 75 2 7 TE buffer 2 4 1 1 pBluescript Banll 50ng 25fmol pUC18 7ag1 1 444bp 5 125ng 5 125fmol DNA 5 DNA 5ul 2 5ul Ligation high 16 30 eE coli JM109 2 X Gal IPTG LB T4 DNA Ligase 16hr 2 8 10 Ligation high 1 1 Ligation high 1 0 5 107 3 T4 DNA Ligase 10 Io Ei 5 10
7. 0 1 2 3 4 5 3 2 Ligation high Ligation high T4 DNA Ligase 1 1 105 02 52 63 12 0 5 50 65 21 1 0 56 61 27 50 66 72 39 ee 1 0 1 1 07X109cfu g pBluescript 3 2 e pBluescript I Hinc1l 100ng 50imoD EcoR 1 2 6 130ng 0 5 25pmol DNA 5 eDNA 5h 2 5h Ligation high 16 30 eE coliJM109 2ul X Gal IPTG LB T4 DNA Ligase 16hr 2
8. 05 06 TOYOBO DNA Ligaton Kit Ligation high Code NoLGK 101 TOYOBO CO LTD Life Science Department OSAKA JAPAN A3274K CO ET 2 1 2 3 Ligation high 1 2 3 5 1 DNA
9. 30min 16 x10min 5 16Cx 1hr 16 x20min 6 16C x16hr 4 e Hindlll 5 PEN 0 e e 6 5 1 e pBluescript I AScal 5ng NaCl TE buffer 5 e DNA 5ul 2 5pl Ligation high 16 30 eE coliJM109 2 LB 2 Ligation high 1 1 Ligation high 1 0 5
10. mail techosk bio toyobo cojp URL http7 wwwtoyobo cojp seihin xrlifescience html
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