SYBR® Premix Reagent Trial Pack
Contents
1. DNA template 100 ng RT PCR cDNA RT template PCR 10 3 25 ul 2 PCR gt SYBR Premix Ex Taq SYBR Premix Ex Taq ll 2step PCR Hold 40 Dissociation 95 C 30 95 C 5 60 30 gt SYBR Premix DimerEraser 3step PCR gt Hold 40 Dissociation 95 C 30 95 C 5 55 C 30 72 C 30 3 Thermal Cycler Dice Real Time System Thermal Cycler Dice Real Time System http wwwr takara bio coJp 5 RR489 lt ABI PRISM 7000 Applied Biosystems 75002. SYBR Premix 2 x 10 ul 1x PCR Forward Primer 10 uM x pl y uM PCR Reverse Primer 10 uM x ul y uM ROX Reference Dye Il 50 X 0 4 ul Template 2 ul lt 100ng 2 dH2O up to 20 ul Total 20 ul Primer x Primer y SYBR Premix Ex Taq 0 4 ul 0 2 uM SYBR Premix Ex Taq ll 0 8 ul 0 4 uM SYBR Premix DimerEraser 0 6 ul 0 3 uM Primer 0 1 1 0 uM template DNA template 100 ng RT PCR cDNA RT template PCR 10 1 2 3 20 ul 2 PCR gt SYBR Premix Ex Taq SYBR Premix Ex Taq Il Holding Stage 95 C 30 SYBR Pre
3. 0 1 1 0 uM 2 template DNA template 100 ng RT PCR cDNA RT template PCR 10 3 20 ul 2 PCR gt SYBR Premix Ex Taq SYBR Premix Ex Taq ll 2 step PCR w Holding Stage 40 Melt Curve Stage 95 C 30 95 C 5 60 20 gt SYBR Premix DimerEraser gt 3step PCR Holding Stage 40 Melt Curve Stage 95 C 30 95 C 5 55 C 30 72 C 30 3 PCR
4. PCR SYBR Premix Ex Taq SYBR Premix Ex Taq II SYBR Premix DimerEraser O SYBR Premix Ex Taq http www takara bio co jp 14 RR489 VII SYBR Premix Ex Tag Tli RNaseH Plus RR420A B SYBR Premix Ex Tag Perfect RealTime RRO41A SYBR Premix Ex Tag 5 mL 25 mL RRO41L RRO41W SYBR Premix Ex Tag 5 mL 25 mL ROX plus RR41LR RR41WR SYBR Premix Ex Tag II Tli RNaseH Plus RR820A B SYBR Premix Ex Tag Perfect Real Time RRO81A SYBR Premix Ex Taq 11 5 mL 25 mL RRO81L RRO81W SYBR Premix Ex Taq 11 5 mL 25 mL ROX plus RR81LR RR81WR SYBR Premix DimerEraser Perfect Real Time RRO91A B PrimeScript RT reagent Kit Perfect Real Time RR037A B PrimeScript RT Master Mix Perfect Real Time RR036A B PrimeScript RT reagent Kit with gDNA Eraser Perf
5. RT PCR amp Perfect Real Time http www takara bio co jp realtime Ill 4 6 4 80 20 4 6 http wwwr takara bio coJp 3 RR489 IV 1
6. Perfect Real Time TEL 03 3271 8553 TEL 077 543 7297 http wwwr takara bio coJp 11 RR489 2 RT PCR RT PrimeScript RT RR037A B RR036A B RR047A B PrimeScript RT reagent Kit Perfect RealTime RR037A 1 RNA g RNA 1 gt 5XPrimeScript Buffer for Real Time 2 ul 1x PrimeScript RT Enzyme Mix 0 5 ul Oligo dT Primer 50 uM 0 5 ul 25 pmol Random 6 mers 100 uM 0 5 ul 50 p
7. 3 step PCR 95 C 5 95 5 55 C 30 55 C 30 72 C 30 72 1 http wwwr takara bio coJp 10 RR489 VI Appendix 1 PCR RT PCR amp Perfect Real Time http wwwrtakara bio cojp realtime RefSeq SYBR Green RT PCR
8. PCR 3 SYBRe Premix PCR 80 150 bp 300 bp 3 SYBR Premix PCR No Template Control 2 ADNA 1 fg 10 fg 100 fg 1 pg 10 pg 100 pg amp 1 300 bp Thermal Cycler Dice Real Time System O SYBR Premix Ex Taq http www takara bio co jp 13 RR489 PCR RRO41 mM RRO81 A RRO91 RRO41 RRO81 RR091 PCR gt SYBR Premix Ex Taq 95 SYBR Premix Ex Taq ll 94 SYBR Premix DimerEraser 86 5 0 10 0 Initial Quantity Log2 O SYBR Premix Ex Taq 2 No Template Control 1 SYBR Premix Ex Taq Il SYBR Premix DimerEraser No Template Control O
9. PCR PCR PCR 95 5 15 PCR 95 30 http wwwr takara bio coJp 4 RR489 lt Thermal Cycler Dice Real Time System gt 1 PCR lt 1 gt SYBR Premix 2 Xx 12 5 ul 1x PCR Forward Primer 10 uM xul yuM PCR Reverse Primer 10 uM xul yuM Template 2 ul lt 100ng 2 dH2O up to 25 ul Total 25 ul Primer x Primer y SYBR Premix Ex Taq 0 5 ul 0 2 uM SYBR Premix Ex Taq ll 1 0 ul 0 4 uM SYBR Premix DimerEraser 0 75 ul 0 3 uM 1 Primer 0 1 1 0 uM 2 template
10. SYBR9 Premix 2 X conc 80 gt 2 3 SYBR Green 4 V SYBR Premix Ex Taq Perfect Real Time SYBR Premix Ex Taq Il Perfect Real Time PCR 2 step PCR SYBR Premix Di merEraser Perfect Real Time 3 step PCR TakaRa Ex Taq Hs Tag
11. http wwwr takara bio coJp 15 RR489 NOTICE TO PURCHASER LIMITED LICENSE P5 PCR Notice Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US 5 994 056 and 6 171 785 The purchase of this product includes a limited non transferable im munity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right under any other patent claim and no right to perform commercial services of any kind including without limitation reporting the results of purchaser s activities for a fee or other commer cial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a separate license from Roche Further information on purchas ing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA L11 SYBR Green I This product is covered by the claims of U S Patent No 5 436 134 and 5 658 751 and their foreign counterpart patent claims Takara PCR products containing SYBR Green are sold under license from Molecular Probes Inc only for the us age in Real time PCR for internal research purpose These products are not to be used for the purpose such as providing medical diagnostic or any other testin
12. 10 4 50 2 PCR gt SYBR Premix Ex Taq SYBR Premix Ex Taq ll gt 2step PCR Holding Stage 40 Melt Curve Stage 95 C 30 95 C 5 60 31 or34 gt SYBR Premix DimerEraser gt 3 step PCR Holding Stage 40 Melt Curve Stage 95 C 30 95 C 5 55 30 72 31or34 7000 31 7500 34 3 PCR http wwwr takara bio coJp 6 RR489 lt Applied Biosystems 7500 Fast Real Time System gt Applied Biosystems 7500 Fast Real Time System 1 PCR 1 gt
13. RR489 ll AS SYBR Premix Ex Taq Perfect Real Time 2 X conc 1 1 ml SYBR Premix Ex Taq ll Perfect Real Time 2 X conc 1 1 ml SYBR Premix DimerEraser Perfect Real Time 2 X conc 1 ml ROX Reference Dye 50 X conc 2 200 ul ROX Reference Dye Il 50 X conc 2 200 ul 1 TaKaRa Ex Taq HS dNTP Mixture Mg2 SYBR Green 2 Life Technologies PCR ABI PRISM 7000 ROX Reference Dye 7500 7500 Fast Real Time PCR System ROX Reference Dye ll Thermal Cycler Dice Real Time System LightCycler ERED 1 PCR authorized instruments 2 3 4 5 PCR WERK PCR VI 1
14. 4 8 80 150 bp 300 bp GC 40 60 45 55 Tm OLIGO 1 63 68 C Primer3 2 60 65 C GC AT 3 T C polypyrimidine RE 17 25 mer Tm A G polypurine 3 3 GCC AT 3 G C 3 T 3 base 3 2 base BLAST 3 1 OLIGO Primer Analysis Software Molecular Biology Insights 2 Primer3 http www genome wi mit edu ftp distribution software 3 http www ncbi nlm nih gov BLAST
15. http wwwr takara bio coJp 8 RR489 Ne RS manny SYBR Premix Ex Taq SYBR Premix Ex 7gg 9 step PCR O No Template Control Ct PCR 100 SYBR Premix SYBRS Premix DimerEraser SYBR Premix Ex Taq
16. SYBR Premix Ex Taq SYBR Premix Ex Taq ll SYBR Premix DimerEraser 0 1 uM 1 0 uM w PCR 2 step PCR 3 step PCR O 3 step PCR SYBR Premix DimerEraser
17. 3 step PCR apres 95 C 5 95 5 55 C 30 60 C 30 64 C 30 72 C 30 60 C 30 72 30 95 C 5 95 C 5 http wwwr takara bio coJp 9 RR489 2 step PCR SYBR Premix Ex Tag SYBR Premix Ex Tag 2 step PCR 95 5 95 C 5 60 30 64C 30 O 2 step PCR SYBR Premix Ex Tag SYBR Premix Ex Taq 2 step PCR 95 C 5 95 C 5 60 30 60 1 95C 5 55 C 30 3 step PCR 95C 5 55 30 72 30 72 1 3 step PCR SYBR Premix DimerEraser
18. DNA 3 1 100 PCR 7aKaRa Ex Taq HS 2 DNA SYBR Green DNA DNA 1 F F 3 http www takara bio co jp 2
19. Real Time System gt 1 PCR 1 gt GAs SYBR Premix 2 X 25 ul 1x PCR Forward Primer 10 uM xul yuM PCR Reverse Primer 10 uM x ul yuM ROX Reference Dye or Dyell 50 X 1 ul Los Template 4 ul lt 200g 3 dH2O up to 50 ul Total 50 ul Primer x Primer y SYBR Premix Ex Taq 1 0 ul 0 2 uM SYBR Premix Ex Taq ll 2 0 ul 0 4 uM SYBR Premix DimerEraser 1 5 ul 0 3 uM 1 Primer 0 1 1 0 uM x 2 ABI PRISM 7000 ROX Reference Dye 50 X 7500 Real Time PCR System ROX Reference Dye ll 50 X 3 template 50 ul DNA template 200 ng RT PCR cDNA RT template PCR
20. ect Real Time RR047A B Vill 1 2005 3 4 PCR 120 126 2 2008 PCR R 3 1 RT PCR 39 43 IX SYBR Molecular Probes Inc
21. g analysis or screening services or providing clinical informa tion or clinical analysis in return for compensations L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries L46 SYBR Melting Curve Analysis The purchase of this product includes a limited non transferable license for all fields other than human or vet erinary in vitro diagnostics under specific claims of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 owned by the University of Utah Research Foundation or Evotec Biosystems GmbH and licensed to Idaho Technol ogy Inc and Roche Diagnostics GmbH to use only the enclosed amount of product according to the speci fied protocols No right is conveyed expressly by implication or by estoppel to use any instrument or system under any claim of U S Patent Nos 6 174 670 6 569 627 and 5 871 908 other than for the amount of product contained herein L52 Rox Reference Dye Research Field Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser s own internal research No other patent rights are conveyed ex pressly by implication or by estoppel Further information on purchasing licenses may be obtained by contact ing the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA M57 LA Technology This product is co
22. ma RR469 IdfaR4 SYBR Premix Reagent Trial Pack v201108Da SYBR Green Assay PCR 3 3 9 10 SYBR Premix Ex Taq Perfect Real Time RRO41 SYBR Premix Ex Taq Perfect Real Time RR081 SYBR Premix DimerEraser Perfect Real Time RR091 Thermal Cycler Dice Real Time System ABI PRISM 7000 7500 7500 Fast Real Time PCR System Life Technologies e LightCycler Roche Diagnostics 1 7aKaRa Ex Tag HS PCR PCR SYBR9 Greenl 1 PCR PCR DNA DNA
23. mix DimerEraser Holding Stage 95 C 30 3 2 step PCR 40 95 3 60 30 3 step PCR 95 C 3 55 30 72 30 40 Melt Curve Stage Melt Curve Stage PCR http wwwr takara bio coJp 7 RR489 lt LightCycler gt LightCycler Roche Diagnostics 1 PCR 1 gt SYBR Premix 2 X 10 ul 1x PCR Forward Primer 10 uM x pl y uM PCR Reverse Primer 10 uM x ul y uM Template 2 ul lt 100ng 2 dH2O up to 20 ul Total 20 ul Primer x Primer y SYBR Premix Ex Taq 0 4 ul 0 2 uM SYBR Premix Ex Taq ll 0 8 ul 0 4 uM SYBR Premix DimerEraser 0 6 ul 0 3 uM 1 Primer
24. mol total RNA RNase Free dH20 Total 10 ul 2 1 Oligo dT Primer Random 6 mers mRNA cDNA Gene Specific Primer Oligo dT Primer 50 UM 0 5 ul 25 pmol Random 6 mers 100 uM 0 5 ul 50 pmol Gene Specific Primer 2 uM 0 5 ul 1 pmol K2 10 ULOR 500 ng total RNA 2 37 C 15 3 85 5 4 C 3 Gene Specific Primer 42 15 PCR 50 cDNA V PCR http wwwr takara bio coJp 12 RR489 3 ADNA
25. vered by the claims 6 16 of U S Patent No 5 436 149 and its foreign counterpart patent claims Takaka Tel 077 543 6116 Fax 077 543 1977 http www takara bio co jp SINT ANS 4l 201108Da
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