SDS-PAGE
Contents
1. 22. 9 DONA RNA
3. UM 0A BA 6 1 3
4. 3 7 TEMED 1 0 TE MED 1 0
5. DNA 1 gt 700bp 5 0 0bp Da kDa N N Bis Bi s
6. PAGE 100mA 500V 2
7. 40 60 LL AEF 6530 T12 5L e 12 5 1m AE 1410 ExzRun SDS 20mA 70min AE 1440 EzStandard AE 1340 Stain 40zz CBB AE 7305 PAGE as a ae sh C C12 5L 12 59 0 75mm PAGEL High 30min
8. 5 20 8 SD S SDS PAGE
9. pH DNA RNA 2 0 A G TT C U R C COO N 3 2 1 0 nm 103 109 2 1 5 1
10. TEMED N NN N 2 7 9zsp TN Bi s 3 20
11. SDS PAGE PAGE 2 1 20mA
12. D 9 ATTO z c 2 3306 400 PAGE 2 AE 7350 PAGE c ATTO AE 1340 EzStain AOua AE 1360 EzStain Silver atto PAGE No 2322260 AE 7350 PAGE 1 68 000 2321826 WSE 1020 NR a 1 86 000 C L cr 10
13. C B B n g CBB G 250 R 2 50 1 0 B ee 0 Zu K Ca 9 ee CBB 2 CB B
14. CBB 300mL 100mL 600mL SDS 0 1g 1 29 AH 0 1mL 1 C 0 5M Tris HC1 pH6 8 1mL 50mM 2mL 20 10mL C B B 1 2mg mL 1 _
15. Tfis 18 2g SDS 0 4g HC1 pH 8 8 100mL C 0 5M Tris HC1 0 4 SDS Iris 6 1g SDS 0 4g HC1 pH 6 8 100mL D 10 100mg 1mL Tris 1 5g 25mM SDS 0 5g 0 1 7 2g 192mM 500mnL 1 PAGE 5 HC1 pH BPB BPB lmg 0 1mL 0 9mL CBB CBB lg 300mL 100mL 600mL
16. 10000 90000 200000 es v ATTO ATTO ATTO PAGE R 1 20 20 100 1 5ml ATTO e PAGEL _ ATTO EzStainA4Qur ATTO EzAppy A 30 29 2g N N 0 8g 100mL B 1 5M Tris HC1 0 4 SDS
17. 0 3 0 6 0 LILHMIIHMHMI 2 0 3 0 ex S DS S DS 100 500rzD 3 1 5 1L C C 2 0mA 1 7 0 9 0 PAGE 8 gL 3 0 6 0 2
18. DNA AE 1360 E Stin Silver 8 1
19. 1 SDS SDS 1 KN 10 20 5 SDS PAGE La emm 1 i La emm 1 i i 15 C17 Tris i HC 1 Tri s 1y
20. 2 4 0 mA 1 mm 2 0 mA 2mm 4 0mA 1 2 1 2 C C Constant Current C V Constant Voltage CBB
21. 111 0D41 3 g 2 D3 5827 BH1 5 D3 582 6647 un 08 5827 4873 098 5827 4874 110 0016 8 g B D8 5818 7580 D3 51B 75B3 530 0044 8 1 0E 6136 1421 DB 8356 3625 EURL http ww atta co jp
22. SDS SD S SD S SD S S DS 2 3 SDS 5 Laenml ii SDS SDS PAGE dH6 8 SDS Web er Os born
23. 3 2 La emm 1 i p
24. IEF a sk 7 9 DU _ 02 La emm1l i 4 SDS Laenml SDS SDS PAGE SDS CH CH OSO Na SDS PAGE SDS S DS 1 1 2 1 5
25. 1 17000 19000 2332346 WSE 7020 ExzProteinLadder SDS PAGE 1 20 000 2332330 AE 1430 EzApply SDS PAGE 1 6 800 2332310 AE 1410 EzRun SDS PAGE 1 4 800 2312200 WSC 2400 atto 1 98 000 2332360 AE 1360 EzStain Silver 1 16 000 2332370 AE 1340 EzStain 4 zz 1 9 800 c 2 000 1 7 DNA AE 1430 EzApply WSE 7020 EzProteinLadder AE 1340 EzStainAQua DNA 2015 5 1 vV D NA
26. ICS 2M 7 La emm1 i SDS PAGE Tris gt y SDS N N Bis TEMED 2 OB 2 250 R 250 B PB pu7 e PAGEL TEMED
27. La emm1i La emm 1 i pH8 8 SDS Tris HC1 Tris 1 y C17 SDS p H C1 2
28. 1 0 0 n g R I WAR 0 ENA OI CCD
29. KIT I BIOSCIENCE amp BIOTECHNOLOGY PAGE Polyacrylamide gel Electrophoresis 4 i BIOSCIENCE amp BIOTECHNOLOGY ki DNA RNA
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