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1. 1 0 05molL pH7 6 2 4 100 87 4 100 49 6 90 1 104 29 2 9 18 4 25 92 6 79 30 33 3 3 10 CV 4 59 4 0 a CD56 C5 9 y 0 9821x RPE 99975 0 3358
2. 1 HBV HIV 2 3 4 5 6 1 2 8 C 2
3. 12 24 PBS PBS 13 4 1 2 14 15 OO NO 1 4 1 1 A ER En RE NE BB 3 PBS 4 BSA
4. nunts a 1 9 101 1 1 3 10t CSErRPE Log Cormp 5 M1 M2 M2 10 35 e 488nm 570nm 2 1 gate 2 3 4 5
5. FCS 100L 100L 5 10gL a 6 NacCl 7 8 0 9 2 4 C 30 10 Vortex 11 3 2mL 12 13 4 300xg 5 14 5 50L 15 2 16 6 29 BSA FCS 0 01mol L PBS 3mL Vortex 7 300xg 5 _ 8 50L 93 9 PBS 196 0 3mL
6. 7 1 2 121C 20 1vol96 3 2 8 C 2 O Code No CD56 C5 9 RPE R7251 1mL 100test Code No lgG2b Mouse RPE X0951 1mL 100test 1 Poggi A CD56 CD Guide In Mason D Andre P Bensussan A Buckley C Civin C Clark E et al editors Leucocyte typing VII White cell differen
7. 4 5 6 10 11
8. Vortex 1 3mL SS o 2 PBS 2 10 3 6mL 2 RE 1 4 1500xg 5 5 a ne eG 6 19 6BSA 196FCS PBS 7 1500xg 5 0 2 8 2 10 L 9 6 8 2 6 6 10 PBS 11 10x10 mL 10gL 3 2 4 C 30 _ 3 2
9. 1030817 0 2009 2 1 877449 21800AMY10100000 NK CD56 C5 9 RPE 1 2 3 4 5 6 Clone lg Type RPE CD56 C5 9 C
10. BSA FCS 0 01mol L PBS 2 4 296BSA FCS 0 01mol L PBS 5 0 5mol L NaCI 1mL 6 1030817 0 1 1 2 0 54 128 192 25B FS Lin 3 Entyhe nrtrn 1n9 1n1 10 1 1 RPE Ln Comp 4 B A
11. D56 C5 9 RPE C5 9 lgG2b z CD56 1 1 CD56 NK CD56 C5 9 R phycoerythrin RPE 1 2 3
12. tiation antigens Proceedings of the 7 International Workshop and Conference 2000 Jun 19 23 Harogate United Kingdom New York Oxford University Press Inc 2002 p 805 806 2 Cost P NK4 CD56 workshop panel report In Kishimoto T Kikutani H von dem Bome AEG Goyert SM Mason DY Miyasaka M et al editors Leucosyte typing VI White cell differentiation antigens Proceedings of the 6 International Workshop and Conference 1996 Nov 10 14 Kobe Japan New York London Garland Publishing Inc 1997 p 271 2 3 Lanier LL Testi R Bindl J Phillips JH ldentity of leu 19 CD56 leukosyte differentiation antigen and neural cell adhesion molecule J Exp med 1989 169 2233 8 4 Saalmuller A et all Summary of the animal homologue section of HLDA8 Cellular Immunology 236 2005 51 58 600 8493 18 TEL 075 211 3674 600 8493 18 TEL 075 211 3675 FAX 075 211 1755
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