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1. MKe7 2012 vor 8 PCR cDNA ReverTra Ace qPCR RT Master Mi DINA PCRH cDNA ReverTra Ace qPCR RT Master Mi with gDNA Remover Be S0 6of 5 1 1 KRT10 S018 1 16 5018 3 30 TNF z ReverTra Ace qPCR RT Master Mix ReverTra Ace 0 Bover2. CAI 30 OFF FAX No 06 6348 3833 03 6422 4951 2012 3 30 1 2012 3 30 CAI Total Kit
3. PCR 50 PCR SS Plus CT SMAC CT RMAC 37C 20 3 5 3 1 2 1 1 2 10096 12 1 99 9596 1987 1988
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5. RNA DNA PCR 2g0C RNA CDNA no RT Control EE Oligo dT Random Primer RNA EE PCH 2 PCR
6. PCR 1 1 M Takagi et al App Environ Microbiol 63 4504 4510 1997 PCR PCR PCR Mouse TBP PCR grade water 11 ul Primer 1 CAGTTGCTACTGCCTGCTGTTGTT SxPCR Buffer for KOD FX Neo ob ul Primer 2 GCTAGGATTAAAGACGTGCCACCA mM dNTPs 10 gl
7. PCR 5 cDNA ReverTra Ace qPCR RT Master Mix Code No FSQ 412o ACTB 20 1 S 100 5 RA HeLa total RNA 100ng 10 60 2 RE 20 PCR 8 1 THUNDERBIRD SYBR qPCR Mix Code No QPS 201 ReverTra Ace A B cDNA 2 0 109 Pe HE 6 Master Mix Target 5 Applied Biosystems 7900HT E RNA RNA HeLa total RNA 2pg 2 g 8 PCRH 5 5
8. NOTICE TO PURCHASER LIMITED LICENSE PCH www toyobo co jp bio ISO 14001 JAB JIC Quality Assurance Ltd IS014001 EBEST RE JA No E004 S0 14001 CMO02 IS01400 1 14
9. RNA cDNA ReverTra Ace qPCR RT Master Mix Code No FSQ 201 HeLa total RNA 2pg 2ug P0I 36 r 34 PCR 32 THUNDERBIRD SYBR qPCR Mix Code No QPS 20 1 30 cDNA2 ul 20 109 Target House Keeping Gene 5 28 TF Applied Biosystems 7900HT RF _ 24 22 2 0 301 33 76 31 16 32 89 32 54 20 1 301 31 43 30 73 27 70 30 05 28 65 20 200 2 301 28 64 27 29 24 44 26 72 25 22 18 L 2 000 3 301 2541 2879 gl2 92391 gl98 20 000 4 301 21 86 20 43 17 69 19 88 18 42 16 200 000 5 301 18 65 17 09 14 14 16 59 15 10 Nl 1 000 000 6 000 16 03 15 03 11 63 14 37 13 09 2 000 000 12 10 1 2 3 4 5 6 7 LOG RNA EE ERY gDNA Remover DNA gDNA Remover DNA Actin PCR
10. lt 3 7 PA RR 3 CytofecH i F PMC LIPmis TNIF IFP al HLr Hf EMHEPCREE KOD Fx Neo L PC F ep FO Fe ah 4
11. DNA DNA DNA DNA BioMasher DNA PCR KOD FX 3 DNA PCR
12. 2096 PCR mRNA 5 2 DNA ReverTra Acey qPCR RT Master Mix with gDNA Remover DNA 20 DNA cDNA ReverTra Ace PCH cDNA a ReverTra Ace qPCR RT ReverTra Ace qPCR RT Master Mix ReverTra Ace qPCR RT Kit Master Mix with gDNA Remover Code No FSQ 101 Code No FSQ 201 Code No FSQ 301 0 Gene Specific Primer no RT Control a por RT buffer 3 mr Master Mix gDNA ee Master Mix Enzyme Mix RT 37C Smn gDNA Remover RT Primer Mix 0 88C 5min 37C 5min S70CTSmn 5xRT Master Mix I 98 0 5min 7 5 15min 98C 5min dPoR lt i dPoR lt MKe7 2012 vor 8 1 cDNA cDNA HeLa total RNA100ng
13. PCR cDNA ReverTra Ace qPCR RT Master Mix with gDNA Remover 2012 1 16 2012 3 30 PCR cDNA ReverTra Ace qPCR RT Master Mix Code No FSQ 20 1 ReverTra Ace PCR 5X RNA PCR cDNA PCR cDNA ReverTra Ace qPCR RT Master Mix with gDNA Femover Code No FSQ 30 1 DNA gDNA Remover RNA DNA cDNA
14. 2 3 www toyobo co jp bio 2012 1 23 2012 3 3 httDp www toyobo co jp seihin xr lifescience tech reader quiz index html 530 8230 2 TEL 06 6348 3786 FAX 06 6348 38393 E mail order lifescience toyobo jp 141 8633 10 TEL 03 6422 4819 FAX 03 642 495 1 E mail order lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 182 00 13 00 17 00 E mail tech_osaka toyODO jp URL http www toyobo co jp bio A4660K 2012 71 21 000 1
15. TA UPLOAD 8 2012 vor 98 HOT PCR PCR PCR PCRH PCR PCR PCR PCR i a ft hE a Ft DNA nh a a PCH 3 310bp DNA 1
16. qPCR RT Master Mix with gDNA Remover cDNA ReverTra Ace qPCR RT Master Mix with gDNA Remover Code No FSQ 30 1 HeLa total RNA 0 1pg 10pg 100pg 1ng 10ng 100ng 1ug gDNA 100ng 20 I PCR THUNDERBIRD SYBR qPCR Mix Code No QPS 201 cDNA 2 17 20 10 Target ACTB 188bp Applied Biosystems 7900HT ReverTra Ace qPCR RT Kit 5xRT Buffer Enzyme Mix 00 10AI Primer Mix Nuclease free Water DNA FSQ 101 38 000 ReverTra Ace qPCR RT Master Mix 5XRHT Master Mix 5xRT Master Mix no RHT Control 00 10AI Nuclease free Water FSQ 0 1 38 000 ReverTra Ace qPCR RT Master Mix with gDNA Remover gDNA Remover 4XDN Master Mix 5XRHT Master Mix I 00 10AI 5xRHT Master Mix I no RT Control Nuclease free Water ReverTra Acee qPCR RT Master Mix Code No FSQ 201 5xRT Master Mix 5xRT Master Mix no RT Control ReverTrg Ace qPCR RT Master Mix with gDNA Remover Code No FSQ 301 5xRT Master Mix I 5xRT Master Mix
17. DNA KOD FX Neo PCR KOD FX Neo PCH PCR KOD FX A Neo PCH PC M 1 2 3 M 1 2 3 M 1 2 3 M 50 ul 4 6 1mm eo my KOD FX Neo 200 bp DNA a _ Mouse TATA box binding protein TBP 0 5 kb
18. ISO 8001 Restriction EndonucleaseS Modifying Enzymes
19. KOD FX DNA 30 40 Ke7 VOL 98 PCR KOD FX Neo PCR KOD DNA polymerase KOD FX PCR KOD FX PCR 65030 gt PCH KOD FX Neo KOD FX gt
20. 4 0 05 0 0296 0 0019 10096 99 95 94 PCHR 1 mlX5 1mlx1 00 1 XPCR Master Mix 10XPrimer Mix UNG Uracil DNA Glycosylase 500 PCR 500 50 5 000 PCR 4 FIK 101 150 000 taku_arakawa toyobo jp 10 MKe7 2012 vor 8 Cell Applications INC CAI
21. cyte 4 cell 5 cell 6 7 8 cell 9 cell SIN Y FIBRO _ VISUAL OOCYTE ADIPOSE HEPATIC EPIDERM CHONDRO AUDITORY LEUCOCYTE OSTEOCYTE DENDRITIC EPITHELIAL f MELANOCYTE LYMPHOCYTE ERYTHROCYTE ENDOTHELIAL MESENCHYMAL KERATINOCYTE SPERMATOCYTE UPLOAD 5 2 000
22. Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit CD4 T Total Kit CD19 BB Total Kit CD14 Total Kit Aortic Endothelial Cells HAOEC Cardiac Fibroblast HCF Internal Thoracic Artery Endothelial Cells HITAEC Coronary Artery Smooth Muscle Cells HCASMC Subclavian Artery Smooth Muscle HScASMC Brachiocephalic Artery Smooth Muscle Cell HBcASMC Carotid Artery Smooth Muscle Cell HCtASMC Pulmonary Artery Smooth Muscle Cells HPASMC Aortic Smooth Muscle Cells HAOSMC Internal Thoracic Artery Smooth Muscle Cells HITASMC Mammary Epithelial Cells HMEpC Astrocytes HA Neural Stem Cells HNSC Bronchial Epithelial Cells HBEpC Tracheal Epithelial Cells HTEpC Lung Fibroblasts HLF Lung Microvascular Endothelial Cells HLMVEC Mononuclear Cells Peripheral Blood HMNC PB Mononuclear Cells Bone Marrow HMNC BM Human Periphery Blood CD4 T Cells HPBTC Human Periphery Blood CD19 B Cells HPBBC Human Periphery Blood CD14 Monocyte cells HPBM To
23. GAPDH 60 PCR Ct 4Ct 4 THUNDERBIRD Probe qPCR Mix 1mlx1 40 P0C QPS 101T 8 500 OR 1 67mix3 200 20C QPS 101 29 000 50X ROX reference dye 1 67mlX3 200 P0C QPS 101 9 000 1 67mlx3 x5 1000 20C QPS 101X5 133 000 THUNDERBIRDY SYBR qPCR Mix 1mlx1 40 QPS 201T 8 500 THUNDERBIRD SYBR qPCR Mix 50X HOX reference dye 57mlx3 00 QPS 0 1 9 000 1 67mlX3 x5 1000 QPS 201X5 133 000 THUNDERBIRD Probe qPCR RT Set QPS101 THUNDERBIRD Probe qPCR Mix 200 1 63 000 ReverTra Ace qPCR RT Kit 200 FSH1U1 THUNDERBIRD Y SYBR qPCR RT Set THUNDERBIRD SYBR qPCR Mix 200 1 RN 63 000 ReverTra Acee qPCR RT Kit 200 FSQ101 50xX ROX reference dye 50 Il qPCR Mix QPS 101X5 QPS 20 1 X5
24. PCR Mouse transferrin receptor Tfrc 1 5 kb PCR 50l 20 mg ml Proteinase K 10 Mouse membrane glycoprotein Thy 1 2 6 kb 2012 3 30 C6648Ne EE KOD EX Neo 00Ux 1 200 0C KFX 201 35 000 KOD FX Neo 1 0U ul 200UX1 x5 1 000 KFX 20 1X5 140 000 XPCR Buffer for KOD FX Neo SmM dNTP 200Ux1 x10 2 000 KFX 201X10 260 000 50I Code No TA KOD TArget Clone Plus 20C TAK 201 16 000 KOD FX Neo DNA TA TArget Clone Plus Code No TAK 201 A
25. PCHR THUNDERBIRD qPCR Mix Taq DNA polymerase PCR x PCR ROX SYBR Green TaqaMan ABIPRISM 7000 ABI PRISM 7700 Applied Biosystems 7300 App jeg Applied Biosystems 7500 THUNDERBIRD SYBR qPCR Mix A Biosystems Applied Biosystems 7500 Fast Applied Biosystems 7900HT Hm en Applied Biosystems StepOne M Applied Biosystems StepOnePlus M LightCy
26. Total Kit Total Kit Total Kit Kit 2012 3 30 Total Kit 4C 20C 80C 4C 20C CodeNo Total Kit Synoviocytes HFLS adult CA408K05a 169 000 131 000 Total Kit Synoviocytes Osteo Arthritis HFLS OA adult CA408OAK05a 169 000 131 000 Total Kit Synoviocytes Rheumatoid Arthritis HFLS RA adult CA408RAK05a 169 000 131 000 Total Kit Chondrocytes HC adult CA402K05a 141 000 115 000 fetal CA402KO05f 144 000 121 000 Total Kit Chondrocytes Osteo Arthritis HC OA adult CA402OAK05a 141 000 115 000 Total Kit Chondrocytes Rheumatoid Arthritis HC RA adult
27. gDNA Remover gt DNA cDNA ReverTra Acee qPCR RT Master Mix with gDNA Remover Code No FSQ 301 HeLa total RNA 0 5 g 10 I gDNA Hemover 4XDN Master Mix gDNA 5xRT Master Mix HI 5xRHT Master Mix I no RT Control PCR THUNDERBIRD SYBR qPCR Mix Code No QPS 201 CDNA 20 1076 Target ACTB 188bp i Applied Biosystems 7900HT MKe7 2 2012 VOL 9 8 Ey DNA ga gd HeLa total RNA DNA DNA ReverTra Acee qPCR RT Master Mix with gDNA Remover DNA
28. 10 80 4 DNA PCR 5 PCR DMY DM domain gene on the Y chromosome DMRT 1 DM related transcription factor 1 Taq DNA Pol KOD FX DMRT1 1 300 bp a DMRT1 1 800 bp DMY 1 000 b a 2 Pp Pp 94C 1 mn 98C 10 sec a 55C 1 min 55C 30 seC DMY F 5 CCGGGTGCCCAAGTGCTCCCGCTGS Oe mw NN a EE MR8 DMY R 5 GATCGTCCCTCCACAGAGAAGAGA 72C 7 min 68C 5 min DW Gb xBuffer 5 mM dNTPs 10 1 2 3 4 M 10 pmol ml Primer F T 5 1 500 DMRT1 1 000 10 pmol ml Primer R T 5 DMY a 1 KOD FX KOD FX 2 KOD FX Sample 100 3 Taq DNA polymerase 4 Tag DNA pol Total 50 M100 Bed aes DNA DNA
29. QPS 10 1 QPS 20 1 5 TaqdMan RHoche Molecular Systems Inc MKe7 2012 vor 8 USERS NOTE KOD FX KOD FX 2 DNA Oryzias latipes 1 1 50mg LN BioMasher J 92 3 50 ul phosphate buffered saline 1 2 G PCR 8 1mM NaaHPO04 1 5mM KHaP04 2 7mM KCI AO
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31. gt 4 1 BL Turapical crclone W ll amUIOOHIU FUE esteverys hurnicane NR amn sll Ka PC fnOFF THUNIDERBIRDFE RgrafTra ee PCR RT Kt 2 i 5 a Ine tla 3 a n WT J Co IM5EnenF 3 CE a Re 4 1 1 1 F i 1 PF i 3 Ty i i F FF Fr Pe _ Wk A 1 Fr er i i PP 2 Ipk p W 3 gt y a WE a 6 ss ft 1 a 1 1 w wm gt Mu
32. 112 000 NEW Total Kit Cryopreserved Preadipocytes HPAd adult heart CA802hK05a 125 000 100 000 adult subcutaneous CA802sK05a 100 000 74 000 Total Kit Epidermal Keratinocytes HEK adult CA102K05a 77 000 52 000 fetal CA102K05f 95 000 68 000 neonatal CA102K05n 9 000 55 000 Total Kit Epidermal Melanocytes HEM neonatal black donor CA104BK05n 96 000 68 000 adult CA104K05a 105 000 79 000 neonatal CA104K05n 92 000 64 000 Total Kit Fibroblasts HDF adult CA106K05a 63 000 35 000 fetal CA106K05f 88 000 60 000 neonatal CA106K05n 80 000 52 000 Total Kit Dermal Microvascular Endothelial Cells CADMEC adult CA100K05a 161 000 115 000 neonatal CA100KO05n 157 000 110 000 Total Kit Dermal Lymphatic Microvascular Endothelial Cels HDLMVEC adult CA100LK05a 197 000 145 000 Total Kit Hair Follicle Outer Root Sheath Cell HORSC adult CA606K05a 166 000 133 000 Total Kit Umbilical Vein Endothelial Cells HUVEC neonatal CA200K05n 63 000 36 000 neonatal pooled CA200pKO05n 60 000 33 000 Total Kit Umbilical Artery Endothelial Cells HUAEC neonatal
33. Mouse Tfrc 10pmol ul Primer 1 lS Primer 1 TGTGGAGGGTCAACGTGGTAGTT TOpmol ul Primer 2 lgul Primer 2 GTGACATTCTCAGGTGGCAGCTT KOD FX Neo Tl ul Mouse Thy 1 Total reaction volume 50 gl Primer 1 CCACAGAATCCAAGTCGGAACTCTTG Primer 2 GTAGCAGTGGTGGTATTATACATGGTG 94C min 9g8C 10sec PCR 68C Amin kb _ 35 cycles PCR KOD FX Neo 4C Hold 35 A B FX 0 PCR PCR M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 M KOD FX TI a 200 bp DNA Mouse TATA box binding protein TBP 0 5 kb Mouse transferrin receptor Tfrc 1 5 kb Mouse membrane glycoprotein Thy 1 2 6 kb PCR 296 5 KOD FX KOD FX Neo KOD FX KOD FX Neo KOD FX Neo
34. CA202K05n 116 000 77 000 Total Kit Umbilical Vein Smooth Muscle Cells HUVSMC neonatal CA250K05n 135 000 99 000 Total Kit Umbilical Artery Smooth Muscle Cells HUASMC neonatal CA252K05n 109 000 73 000 Total Kit Coronary Artery Endothelial Cells HCAEC adult CA300K05a 157 000 123 000 Total Kit Subclavian Artery Endothelial Cell HScAEC adult CA3010K05a 177 000 138 000 Total Kit Brachiocephalic Artery Endothelial Cell HBcAEC adult CA3012K05a 171 200 139 000 Total Kit Carotid Artery Endothelial Cell HCtAEC adult CA3014K05a 173 000 139 000 Total Kit Pulmonary Artery Endothelial Cells HPAEC adult CA302K05a 136 000 109 000 UPLOAD 0 vo 98 1 1 Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit Total Kit
35. 4x102 1 100 4x103 _ 4x104 PCR 1 PCR 2 12 8 4 1 988 6 000 2 1 50 PCHR
36. CA402RAK05a 141 000 115 000 Total Kit Osteoblasts HOb adult CA406K05a 154 000 120 000 fetal CA406K05f 160 000 129 000 Total Kit Osteoblasts Osteo Arthritis HOb OA adult CA406OAK05a 159 000 124 000 Total Kit Osteoblasts Rheumatoid Arthritis Hob RA adult CA406RAK05a 159 000 124 000 Total Kit Skeletal Muscle Cells HSkMC adult CA150K05a 168 000 137 000 fetal CA150K05f 157 000 118 000 Total Kit Placental Epithelial Cells HPIEpC fetal CA230K05f 143 000 113 000 neonatal CA230K05n 143 000 113 000 Total Kit Human Cervical Epithelial Cells HCvEpC adult CA232K05a 179 000 144 000 NEW Total Kit Human Renal Proximal Tubular Epihelial Cels HRPTEpC adult CA930K05a 140 000 105 000 EW fetal CA930K05f 140 000 105 000 NEW neonatal CA930K05n 140 000 105 000 NEW Total Kit Human Prostate Epithelial Cells HPrEpC adult CA934K05a 149 000 115 000 NEW Total Kit Human Bladder Epithelial Cells HBIEpC adult CA938K05a 141 000 106 000 NEW Total Kit Human Urethra Epithelial Cells HUEpC adult CA9310K05a 147 000
37. Goyogo T 0 Y 0 B 0 0 C H E M frand Ne item PCH cDNA ReverTra Ace qPCR RT Master Mix DNA PCR cDNA ReverTra Ace qPCR RT Master Mix ReverTra Ace qPCR RT Master Mix with gDNA Remover ReverTra Ace qPCR RT Master Mix with gDNA Remover p 1 3 HOT ITEM 4 PCR THUNDERBIRDY qPCR Mix USER S NOTE 6 KOD FX TECHNICAL REVIEW 7 PCR TKOD FX Neo PCR HOT ITEM 9 PCR PCR CAMPAIGN Total Kit 12 INFORMATION 13 6 6 6 060 0999999099039 14 14 PCR cDNA ReverTra Ace qPCR RT Master Mix 5 Ore DNA
38. I no RT Control THUNDERBIRD qPCR Mix p 4 THUNDERBIRD Probe qPCR RT Set I THUNDERBIRD Probe qPCR Mix 200 ReverTra Acee qPCR RT Master Mix 200 FSQ 301 QPS101 FSQ 0 1 40 000 63 000 THUNDERBIRD SYBR qPCR RT Set I THUNDERBIRD SYBR qPCR Mix 200 ReverTra Ace qPCR RT Master Mix 200 THUNDERBIRD Probe qPCR RT Set THUNDERBIRD Probe qPCR Mix 200 ReverTra Ace qPCR RT Master Mix with gDNA Remover 200 THUNDERBIRD SYBR qPCR RT Set I THUNDERBIRD SYBR qPCR Mix 200 ReverTra Ace qPCR RT Master Mix with gDNA Remover 200 THUNDERBIRD 50I SYBR Molecular Probes Inc MKe7 2012 VOL 9 8 3 QPS201 FSQ 01 QPS101 FSQ301 QPS201 FSQ301 63 000 65 000 65 000 HOT 4 THUNDERBIRD qPCR Mix 20 1 3 30
39. O _ PCR a PCR a EHEC 0157 cfu g 0 Ax AKT TAK AROR SN EC PCR 50 2 cu g 1 10 20 40 80 70 80 80 100 7x105 a 2X108 a x1042 ET MIO FE ce EHEC 0157 EHEC 0157 7x103 LU EE bu
40. PCH NU NU pn __ PY EHEC 0 1 5 BCR 100 PCRH PCR 100 PCR 1 cfu g 0 XOXO ZN00 2XN00 Z
41. Tra Aces PCR qPCR RT Master Mix A B 5 x RSNA HeLa total RNA 100ng PCR cDNA PCR 5 PCR cDNA 1 Photoreceptor cell cell 2 ic cell al growth factor 3
42. cler 1 x LightCycler 2 0 LightCycler 480 Bio Rad MJ iCycleriQ Mx3000P Stratagene Mx3005P Mx4000 TaKaRa Thermal Cycler Dice BioFlux Line Gene Roche Diagnositics ie BeerEvEh mismm re mmwesmali3p PCHR SYBR Green I THUNDERBIRD qPCR _ Mix PCHR PCH PCHR 1 THUNDERBIRD SYBR qPCR Mix A Total_ RNA ReverTra Ace
43. qPCER RT Kit cDNA G3PDH PCR A MKe7 4 2012 voL 98 THUNDERBIRD qPCR Mix B 1 C 100 6 SYBR Green I GI cDNA 3 TaqMan GAPDH cDNA Roche Diagnostics LightCycler 1 1 Roche Diagnostics LightCycler 1 1 mf lo Pl END Eri 24 5 24 0 Ls slope 3 26 1 00W 23 5 RSQ 0 9956 23 0 22 5 2 1 EE 22 0 21 5 ee i Li mm Cyele 20 5 1 00 0 90 0 80 0 70 0 60 0 50 0 40 0 30 0 20 0 10 0 00 Delectar SYBR PatlaRnys_Cycm Thmesholn3n Log 2 1 414 4 SYBR Green I cDNA 29 Applied Biosystems 7900HT HeLa Total RNA CDNA 295 1 414 7 N 4
44. tal Kit 4 ni Ui 4 A 7 adult adult fetal adult adult adult adult adult adult adult fetal adult adult fetal fetal adult adult adult fetal adult adult fetal adult adult adult Code No CA304K05a CA306K05a CA306K05f CA308K05a CA350K05a CA3510K05a CAS3512K05a CA3514K05a CAS352K05a CA354K05a CAS354K05f CA358K05a CA830K05a CA882K05f CAHS820K20f CA502K05a CA504K05a CA506K05a CA506KO05f CA540K05a CA690PBK100a CA690BMK50f CA6902K100a CA6904K20a CA6906K50a 144 000 167 000 167 000 177 000 152 000 168 000 169 000 169 000 144 000 169 000 169 000 173 000 149 000 145 000 163 000 161 000 161 000 145 000 145 000 174 000 57 000 63 000 6 000 109 000 101 000 116 000 132 000 132 000 138 000 116 000 133 000 132 000 132 000 105 000 132 000 132 000 138 000 115 000 115 000 142 000 126 000 126 000 110 000 110 000 130 000 45 000 52 000 NEW 65 000 NEW 100 000 NEW 92 000 NEW CAI Lot
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