2009年7月作製
Contents
1.2. lt lgG lt lgG lt
3. 20 25C 2
4. 242277 AP AP AP AP AO lgG IFN 7OOG Laermmli Sample Buffer 2 Electrophoresis Power Supply EPS 1001 GE 2 203 2 SeeBlue Plus2 Pre Stained StandardlInvitrogen BIO RAD STC 8SOS Q PAGE mini 12 5 1 0mmx12well Tris BES
5. 30 2 15 V 1 IV 1 1 4C 2 1 3 3 4 30 5 3 5 20 5 3 6 100 mM Tris pH 85 15
6. 1 2 7 L pp 10 g mL 1g mL O 5g mL 20L 3 1 6O mA 1 cm TT 1 2 30
7. 50O 10 2 Owg g 10ug g 10 g g 90 14 11 6 1106OO1 FASPEK O g g 10prg g ie 1e ie7ie ie71e ie 16 er1e CO MN
8. 2 A A B B C 1 1 1 17 A A 3 Tris BES 10Ox 10 40Ox 400 4 1Ox Tris Glycine 7 2 1 1Ox TBS 10 Tween 2O OO5 6 O 1 7 E
9. 7 10 8 1 5 9 MW 5O OOO Oug g 110g g 8
10. gt 6O mA 15 V 1 1 4C 1 5 3 30 5 3 20 5 3 100 mM Tris pH 9 5 15 3 10 15 2 8C 2
11. 1OO 8 VECTASTAIN ABC AP Rabbit lgG kit lgG 1O OOO 9 VECTASTAIN ABC AP Rabbit lgG kit A B 10 mL 1 30 1 0 Alkaline Phosphatase Substrate Kit IM lt BCIP NBT gt 1 2 3 100 mM Tris pH 9 5 10 mL 2 1 1 1 1 g 50 mL 19 mL 2
12. 12 GO 1 10 3 cm 3 pH pH 60C8O pH 4 3 OOO Xg 20 5 1 2 5 2 1 1 2 5 2 D 10ug mL 1g mL 0 5ug mL 1 TIT
13. 1Ox 40Ox SD BIO RAD Hybond P PVDF GE BIO RAD 1Ox Tris Glycine BIO RAD 10x TBS BIO RAD Tween 20 VECTASTAIN ABC AP Rabbit lgG kit VECTOR Alkaline Phosphatase Substrate Kit IMV lt BCP NBT gt VECTOR 100 mM Tris pH 9 5 1 2 GE 2 4 VECTOR LABORA TORIES Inc 1 Laemmli Sample Buffer 2 19 1
14. MORINAGA 2010 3 3 FASPEK MIoBS 2 1 16 236 0003 http www miobs com PN 20 7 4O 55mL 1 1O g mLD 2 4 OOOL 1 20 1 14 11 6 1106OO1 FASPEK
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