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Viable Salmonella Selection Kit for PCR

Contents

1. V 1 2 Solution B sal DNA DNA 3 Solution A sal http www takara bio coJp 5 7711 VI 1 EMA 1 EMA
2. EMA b 2 EMA EMA 5 3 http www takara bio coJp 1 1 7711 VII A PCR EMA DNA PCR
3. Viable Bacteria Selection Kit for PCR DNA EMA DNA PCR EMA DNA PCR EMA http www takara bio coJp 2 7711 50 1 Solution A sal 250 ul Xx 2 2 Solution B sal 200 ul x 4 3 Protease Solution 1 150 ul 4 Protease Solution 2 500 wl i Cc Solution A sal EMA DNA Solution B sal EMA
4. DNA PCR DNA Viable Sa monella Selection Kit for PCR EMA DNA EMA DNA Cycleave PCR 5g 77o7e a Detection Kit Ver 2 0 CY205 PCR DNA DNA EMA oe Ss ey W Wi i W ea PCR DNA 1 EMA PCR Control Test Kit Viable Bacteria Selection CY290 Viable Bacteria Selection Kit for PCR EMA
5. 100 ul 40 ul B EMA 4 EMA 40 B 6 C NucleoSpin Tissue XS DNA EMA 2 5 9 45 ml 9 10 109 1 ml 10 ml 10 3 000 Xg 4 5 1 ml Protease Solution 1 3 ul
6. 12 1 5 ml 13 Buffer BE 20 u 11 000 X g 1 DNA 14 DNA 20 ul 1 Proteinase K 740901 50 50 Proteinase K 20 mg 1 vial Proteinase Buffer 1 ml Proteinase K 20 2 Buffer B5 Wash Buffer B5 concentrate 2 ml 8 ml D PCR C 13 EMA EMA DNA CycleavePCR Salmonella Detection Kit Ver 2 0 PCR CycleavePCR Salmonella Detection Kit Ver 2 0 VIII VII 2 E EMA EMA Control Test Kit Viable Bacteria Selection DNA
7. Cycleave Thermal Cycler Dice Iaf4R4 Tel 077 543 6116 Fax 077 543 1977 http www takara bio coJjp v201408Da
8. EMA t ACt EMA EMA At 2 4 EMA EMA DNA 3 ACt 4 EMA Ct ACt EMA ACt 8 15 EMA 3 103 cfu Ct
9. EMA B EMA 1 EMA 2 EMA 1 3 C DNA 95 5 DNA TT D E EMA PCR Control Test Kit EMA 4 3 EMA 3 lt EMA 1 week http www takara bio co jp 10 7711 1 1 EMA DNA EMA
10. EMA EMA EMA EMA EMA EMA EMA EMA EMA LED Crosslinker 12 EM200 LED Crosslinker 1 5 ml 40 ul
11. 4 5 5 15 2 6 95 5 A 4 40 ul 15 ul 6 1 2 EMA 3 lt 5 5 15 Ce gt PRS500W 20 cm 5 5 http www takara bio coJp 13 7711 C NucleoSpin Tissue XS DNA 1 B 6
12. DNA gt 2 2 EMA DNA gt 3 3 EMA EMA a EMA EMA b EMA EMA EMA EMA a HS AAs ee i a a EMA b 1
13. 1 2 4 x 107 lt 4 X 10 lt EMA lt EMA gt lt EMA gt 3 10 102 10 EMA lt EMA gt lt EMA gt 107 EMA http www takara bio coJp 15 7711 IX Xl 1 Nogva HK Dromtorp SM Nissen H Rudi K Ethidium monoazide for DNA based differentiation of viable and dead bacteria by 5 nucl
14. Protease Solution 1 Protease Solution 2 Protease Solution 1 20 http www takara bio coJp 3 7711 Il 1 5 ml EMA DNA LED Crosslinker 12 EM200 LED Crosslinker 2 95 1 5 ml 3 DNA NucleoSpin Tissue XS 740901 10 50 250 4 99 56 70 PCR CycleavePCR Salmonella Detection Kit Ver 2 0 CY205 PCR Thermal Cycler Dice Real Time System
15. CycleavePCR Sa monella Detection Kit Ver 2 0 PCR DNA DNA EMA DNA 121 20 2 59 EMA
16. 1 lahaha Viable Salmonella Selection Kit for PCR v201408Da PCR DNA Viable Bacteria Selection Kit for PCR EMA PCR DNA EMA ethidium monoazide EMA DNA EMA DNA PCR DNA PCR
17. Solution A sal 10 ul Solution B sal 5 a 15 ON 95 5 oO un Ru 1 VII 2 EMA EMA 3 5 EY 5 15 http www takara bio co jp 6 7711 2 EMA EMA 1 2 3 EMA EMA 5
18. t At 12 3 log 4 log VIII 2 10 EMA EMA 3 EMA 1 3 EMA 1 2 2 3 EMA 2 http www takara bio coJp 8 7711 Gt Ct EMA AC t 103 105 107 cfu EMA AC t EE 0 103 10 5 107 Cfu 103 cfu EMA 1 3
19. 55 65 ul Buffer T1 160 ll 2 Proteinase K 16 5 X 2 3 56 10 4 Buffer B3 160 5 X 2 CS 5 70C 5 6 96 100 160 ul 5 X 2 7 NucleoSpin Tissue XS Column Collection Tube 2 ml 8 6 11 000 Xx 9 1 9 Collection Tube 2 ml 10 Buffer B5 2 50 11 000 X 9 1 Collection Tube 11 Buffer B5 2 50 11 000 X 9 2
20. TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Thermal Cycler Dice Real Time System Software Applied Biosystems 7500 Fast RealTime PCR System Life Technologies 1 PRS500W 2 3 DNA LoBind 1 5 ml PCR clean Eppendorf Code 95295 1 5 mI SARSTEDT Code 72 692 100 4 NucleoSpin Soil 740780 10 50 250 http www takara bio coJp 4 7711 IV EMA
21. 10 cfu EMA 2 3 Ct 3 http wwwr takara bio coJp 7711 2 1 1 EMA EMA EMA EMA EMA EMA EMA Control Test Kit EMA 2 EMA EMA A EMA
22. 15 A 104 106 108 cfu ml 104 106 108 cfu ml B EMA 1 EMA 2 EMA 1 3 3 1 2 3 C DNA 95 5 DNA D PCR 2 http www takara bio coJp 7 7711 3 EMA EMA 3 EMA t EMA t ACt
23. EMA qPCR EMA Control Test Kit Viable Bacteria Selection VIlI 95 5 EMA NucleoSpin Tissue XS DNA DNA CycleavePCR Sa monella Detection Kit Ver 2 0 PCR http www takara bio coJp 14 7711 1 3 Xx 107 3 x 102 EMA lt EMA gt lt EMA gt 6 ee 15 Pe ie E ea HH EE Or rr rr hr
24. Protease Solution 2 10 ul 37 1 5 3 3 000 Xx g 4 5 100 100 ul 40 ul B EMA 4 40 15 25 ul B EMA 6 C NucleoSpin Tissue XS DNA http www takara bio coJp 12 7711 B EMA EMA VI EMA EMA LED Crosslinker 12 EM200 LED Crosslinker 1 1 5 ml A 40 ul 2 Solution A sal 10 ul 3 Solution B sal 5
25. ease PCR Biotechnigques 2003 Apr 34 4 804 8 810 812 3 LED Crosslinker12 EM200 Control Test Kit Viable Bacteria Selection CY290 Cycleave PCR Salmonella Detection Kit Ver 2 0 CY205 NucleoSpin Tissue XS 740901 10 50 250 NucleoSpin Soil 740780 10 50 250 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Viable enterohemorrhagic co Selection Kit for PCR 7712 Viable Campylobacter Selection Kit for PCR 7713 Viable Legionella Selection Kit for PCR Ver 2 0 7714 Viable Bacteria Selection Kit for PCR Gram Negative 7700 Viable Bacteria Selection Kit for PCR Gram Positive 7705

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