登録検査機関の登録に関する説明会配布資料（PDF：942KB）
Contents
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111. 2 DNA DNA 3 3 PCR 1 PCR DNA PCR 2 2 400 2 400 1 200 2 PCR DNA E DNA DNA PCR DNA DNA DNase
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113. 3 4 5 6 7 8 9 24 3 1 1 2 3
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130. 1 2 3 4 1 2 3 4 5 6 7 8 9 20 1 1
131. 170bp PCR DNA PCR 1 PCR DNA 157bp PCR PCR 171bp CBH351 2 DNA 3 DNA Zein
132. Bt 1 0 eli TEL 03 3502 8111 03 3502 8097 3160 3170 3171 16 OBt 11 tt 10 Bt 1 1 B t 1 0 Bt1 1
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140. 500 QIAshredder spin colum 10 000Xg 4 15mL 1 5 AP3 500zL mini spin colum 10 000Xg 1 500 prL mini spin column AW 500L 10 000Xg 1 3 mini spin colum 10 000Xg 20 mini spin colummn 10 65 70L 5 10 000Xg 1 DNA
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145. 9 11 11 21 90 16 2 19 AIA 2 15 6
146. DNA DNA Zein PCR buffer II Bt10 F primer JSF3 5 CAC R primer JSR3 5 GGG Taq DNA 0 ACA GGA GAT TAT TAT AGG G 3 AAT AAG GGC GAC ACG G 3 AmpliTaq Gold DNA 16 4 PCR GeneAmp PCR System 9700 5 LA Zein 1 F primer Zein n 5 5 CCT ATA GCT TCC CTT CTT R primer Zein n 3 5 TGC TGT AAT AGG GCT GAT GA 3 2 3
147. 3 2 3 5 30 6 2 9 cp4 epsps Medicago sativa Q163 OECD UI MON 00163 7 lt 3 23 5 30 6 29 cp epsps X cp4 epsps Medicago sativa L JI101 x J163 OECD UI MON 00101 8 x MON 00163 7 3 23 5 30 6 29 cp epsps Gossypium hirsutum L MON88913 OECD UI MON 88913 8 6 9 7 8 8 8 mEPSPS Zea mays subsp mays L Iltis GA21 OECD UI MON 00021 9 7 2 1 8 30 9 29 gt pat crylAb Zea mays subsp mays L Iltis 125xMONS810 OECD UI ACS ZM003 2x MON 00810 6
148. 9 T45 OECD UI ACS BN008 2 11M 97 4 2 3 94 RF3 OECD UI ACS BN003 6 410y7 2 4 9 MS8RF3 OECD hy UI ACS BN005 8 Xx ACS BN003 6 11 5 94 RT73 OECD UI MON 00073 7 6 9 RT200 OECD UI MON 89249 2 7 9 Topas19 2 OECD UI ACS BN007 1 HCN92 O II 141 7 4 HCN10 8 2 MSIRF2 OECD O 7 UI ACS BN004 7 x ACS BN002 5 PGS 2 PHY36 PHY23 9 MSIRF1 OECD O O 141 97 4 UI ACS BN004 7 Xx ACS BN001 4 PGS1 PHY14 PHY35 10 ht 4 OXY 235 OECD UI ACS BN011 5 O I
149. 1 7 2 2 4 3 1 6 9 0 8 4 17 2 23 1 5 9 7 1 8 3 1 9 4 1 5 1
150. Bt10 F primer Bt11 3 5 R primer Bt11 3 3 3 5 AAA AGA CCA CAA CAA GCC GC 3 5 CAA TGC GTT CTC CAC CAA GTA CT 3 Bt10 PCR Bt10 DNA Btll Bt10 Bt10 EE Bt11 17 1 2 1 1 5 9 7 3 1 1 3
151. PCR DNA 1 1 3 4 1 3 4 4 PCR Zein 3 Zein H Bt DNA 1 10 PCR 127bp H HH 157bp PCR 130bp PCR PCR Bt10 HH Bt10 2 DNA gt
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155. A7 Bt10 19 Q8 A8 Bt10 Bt10 Bt10 Bt10
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157. 2 1 CBH351 Bt10 2 1 0 0 gt 100kg
158. PCR DNA B DNA DNA CBH351 Zein PCR DNA PCR PCR PCR buffer II CBH351 F primer CaM03 5 5 CCT TCG CAA GAC CCT TCC TCT ATA 3 R primer CBHO2 3 5 GTA GCT GTC GGT GTA GTC CTC GT 3 Taq DNA AmpliTaq Gold DNA PCR 1
159. DNA AP1 QIAGEN DNeasy Plant Mini Kit AP2 QIAGEN DNeasy Plant Mini Kit AP3 QIAGEN DNeasy Plant Mini Kit AP3 AP3 4 96 100 1 2 AP3 6 mini spin column
160. 0 5mL 1 5mL hAgri Screen R Cry9C 2 2 10 2 1 1 2 400 1 1 10 PCR DNA 2 400 2 400 2 3 1 DNA
161. CTAB CTAB PCR DNA DNA DNA PCR DNA CTAB QIAGEN DNeasy Plant Mini Kit QIAGEN DNeasy Plant Maxi Kit Promega Wizard DNA Clean up System DNA
162. 16 cp 74c OossypZ7 O1O 2004 hirsutum L 531 OECD UI MON 00531 6 11 22 2001 2003 531 17 11 733 DFR Diathus O O 2004 caryophyllus L OECD UI FLO 07442 4 12 10 5 7 18 11363 7733 DFR Diathus O 2004 caryophyllus L OECD UI FLO 11363 1 12 10 59 7 19 123 2 38 73 5H DFR Diathus 2004 caryophyllus L OECD UI FLO 40644 4 12 10 5 7 20 123 8 8 7733 DFR Diathus O O 2004 caryophyllus L OECD UI FLO 40683 1 12 10 7579 21 2004 cp4 epsps Agrostis stolonifera L ASR368 12 10 OECD UI SMG 36800 2 2004 12 10 2003 11 30 22 99 7 9 2004 2003 2003 ery3Bbl cp4 epsps Zea mays subsp mays L O1O1IO 12 10 MON863 Xx Iltis MON86
163. 3 1 1 v y 24 1 v v TE TE 10mmo1 L Tris pH8 0 1 mmo1 L EDTA pH8 00 3 1 2 QIAGEN DNeasy Plant Mini Kit 2 g 50mL 65 AP1 10mL RNase A 20L 65C 15 2 3 AP2 3 250 7L 10 4 000Xg 4 20
164. gt 2 i O O 3 1 RE 1 2 2 F
165. 2 2 4 F 1
166. GeneAmp PCR System 9700 13 5 Zein F primer Zein n 5 5 CCT ATA GCT TCC CTT CTT CC 3 R primer Zein n 3 5 TGC TGT AAT AGG GCT GAT GA 3 3 4 PCR DNA 3 4 1 TAE 100mL 5 gL 10mg mL 50 DNA
167. P CR 1 1 5 2 18 3 1 3 18 3 2 2 4 18 3 3 5 4 9 2
168. 3 9 Bt11 OECD UI SYN BTO11 1 Btll O Bt11 2 4 9 GA21 OECD UI MON 00021 9 5 y f3 hyEn03y CBH351 O A 2 9 6 Eh T14 OECD UI ACS ZM002 1 O O 141 2 y7 X 7 7 AMANO x MON810 O OECD UI MON 00021 9 x MON 00810 6 8 9 Event176 OECD UI O SYN EV176 9 9 39 y hyE03y DBT418 OECD UL O DKB 89614 9 10 T25 x MON810 O OECD UI ACS ZM003 2 x MON 00810 6 1 7 4 MS8 OBCDUI ACS BN003 8 11 2 97 4 2
169. PCR RO Mil11i Q 17MQ cm 121C 15 DNA DNase JAS 2 3 1 DNA 2 400 3 2 DNA DNA DNA CTAB
170. CBH351 F primer Cry9C 5 5 TAC TAC ATC GAC CGC ATC GA 3 R primer 35Ster 3 5 CCT AAT TCC CTT ATC TGG GA 3 15 i2 a EPE A Eee 2 Ed 2 1 2 3 Bt10 PCR PCR 1 PCR 1 1 DNA PCR 2 PCR 1 1 3 2 400
171. 1 GIPSA CSL TSTA 2 3 14 1 2
172. 2 1 2 1 2 3 1 1
173. 5 1 2 6 1 2 SN 3 1 4
174. ee RO 2 1 lt Trait gt Bt9 Corn Grain 5 Minute Test Kit Part 7000012 2 1 1 800 500mL 1 25 1020 mL Trait Bt19 0 5mL 1 5mL 2 8 2 Ed 1 2
175. 0 ha 10 ha 7 10 ha P 0 5 ha 99 20 2 3 ha 5 ha 120 ha 9 tH 742 1 620 ha 7 5 ha 500 ha NEO03 10 ha 1 4 50 ha 9 h 30 ha 2004 2w wl s tt 16 479 100 0 seml eo coml so il ce ha
176. 1 4 25 2005 425 2007 3 31 35 cp epsps Glycine 2005 2001 2003 max L Merr 40 3 2 OECD UI 5 25 MON 04032 6 36 7Cm 2005 Opzg sg vg L 7 Crp 1 1 0 5 2 2005 5 25 2007 12 31 37 DEF Oryza 2005 sg yg L AD4 1 25 2005 5 25 2006 10 31 38 DEF Omxza 2005 sativa L AD4 8 5 2 2005 5 25 2006 10 31 39 DEF Omxza 2005 sg yg L ADS 1 25 2005 5 25 a 2006 10 31 40 DEF QOryza 2005 sg yg L AD7 7 25
177. 3 x NK603 OECD NK603 UI MON 00863 S x MON 00603 6 23 9 72 cy74c cry24b Gossypium O O 2004 2002 2003 hirsutum L 15985 OECD UI MON 15985 7 12 10 15985 24 79 cp4 O1O 2004 2003 2003 epsps crylAc Gossypium hirsutum L 1445 X 531 12 10 1445 Xx OECD UI MON 01445 2 x MON 00531 6 531 25 y NyE03y O1OIO 2003 2002 2002 crylF pat Zea mays subsp mays L Iltis 3 2 B t CrylF maize line 1507 OECD UI DAS 01507 1 gt 26 7 O O 2005 2003 2003 crylAC cry24b cp4 epsps Gossypium hirsutum L 3 2 15985 x 15985 X 1445 OECD UI MON 15985 7 x 1445 MON 01445 2 27 9 OOIO 2005 2004 2003 crylF 3 25 pat cp4 epsps Zea mays subsp mays LL Iltis 1507xNK603 OECD UI DAS 01507 1x MON 00603 6
178. 4mL 50mL 10 3 4mL 50mL 5 1mL 11 AP3 Et OH 10 DNeasy Spin Column colorless 3 000Xg 5 AW 12mL 3 000Xg 15 50mL 65C 1 mL 5 3 000Xg 10 2m 10 5 12 000Xg 4 C 15
179. I 11 y7 1 gt 1 1 2 55 1 O 7 3 1 OECD UI OECD Unique Idetifier OECD 2 OECD UI 1 3 12
180. Xx MON 00863 3 MONS863 5 0 cp4 epsypy Gossypium O 2004 hirsutum L MON88913 6 11 2004 6 11 2004 12 31 6 39 7 oO 2004 crylF pat Zea mays L TC6275 OECD UI 6 11 DAS 06273 8 2004 6 11 2005 3 31 7 a O4547 Oryza sativa L O 6 11 2004 6 11 2003 7 30 8 OQ4S4ID Oryza sativa L O 2 6 11 2004 6 11 2003 7 30 9 OsG42ox7 Oryza sativa G 3 3 22 2004 6 11 2004 6 11 2003 3 31 10 4 OsgA77 Oryza sativa L 2004 B 4 1 18 6 11 2004 6 11 2003 3 31 11 cpZ epsp
181. s oo 2004 2001 Zea mays subsp mays L Iltis NK603 OECD UI 11 22 NK603 2003 MON 00603 6 12 cz2 epsps O1O 2004 Gossypium hirsutum L 1445 OECD UI 11 22 2001 2003 MON 01443 2 1443 13 pg7 Zeg O1OIO 2004 mays subsp mays L Iltis T25 O0BECD 11 22 2001 2003 UI ACS ZM003 2 T23 11 2 7 14 O1OIO 2004 cp epsps crylAb Zea mays subsp 11 22 2003 2002 mays L ts NK603 X MON810 OECD UL NK603 x MON 00603 6 X MON 00810 6 MON810 15 9 O1OIO 2004 cy3P 7 crylAb cp epsps Zea mays 11 22 2004 2004 subsp mays L Ilis MON863 Xx MON810 x NK603 MON863 Xx OECD UI MON 00863 5 Xx MON 00810 6 Xx MONS810 x MON 00603 6 NK603
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