(O111/O121) Typing Kit
Contents
1. Cycleave Thermal Cycler Dice TaqMan Roche Diagnostics GmbH Idf4R4 Tel 077 565 6999 Fax 07 565 6995 http www takara bio coJjp v201510Da2. PCR 0 B PCR PCR DNA B 0111 0121 PCR Positive Control http www takara bio co jp 15
3. 3 1 2 3 PCR PCR Auto
4. om o21 dH2O 5 hn PCR 5 PCR lec http wwwr takara bio coJp 14 CY238 O111 FAM 0O121 ROX 1 dH20 dH20 0121 O111 B 3 PCR A O111 0121
5. Speed FAM ROX FAM ROX gt http wwwr takara bio coJp 7 CY238 lt gt lt none gt NC Negative ControD PC Positive Control UNKN Unknown 1 lt 1 2 3 2 ji ot nem kr a AWT WI TT I PE g 7 i n 1 17 UMW
6. ROX 0O111 0121 PCR RNA DNA RNase H RNase H RNA 1 G RNase H J 2 RNaseH ws
7. PCR Manual ES http wwwr takara bio coJp 4 CY238 V 1 DNeasy Blood amp Tissue Kit DNA 2 PCR 3 a J PCR 4 V 1 2 DNA DNeasy Blood amp Tissue Kit QIAGEN DNeasy Blood amp Tissue Kit DNeasy Blood amp Tissue Kit NucleoSpin Tissue 740952 10 50 250 V 2
8. FAM ROX target http wwwr takara bio coJp 13 CY238 VI Ct 40 Ct 40 Ct 40 Ct 40 lt A gt O DNA 2 FM EE mm 02 W a B PCR O111 O121 B PCR lt B PCR gt PCR 100 Positive Control DNA lt A gt O111 0121
9. 2 lt A gt 0 DNA B PCR gt PCR 1 100 Positive Control DNA B gt A gt 0O111 0121 VT V 1 PCR B gt 1 Positive Control lt B gt 3 O111 Positive Control 0121 Positive Control EASY Dilution for Real Time PCR 17100 O111 Positive Control 1 O111 Positive Control 5 EASY Dilution 45
10. RNA 1 http wwwr takara bio coJp 2 CY238 20 25 ul 1 2XCycleave Reaction Mixture 2 O111 0121 Primer Probe Mix 3 dH2O 4 O111 Positive Control 5 O121 Positive Control 6 EASY Dilution for Real Time PCR 2 COnC 5 COTC 250 ul 100 ul 1 ml 40 8 40 uI 8 1 ml 1 lt 3 4 6 2 x Cycleave Reaction Mixture PCR Buffer dNTP Mixture 0111 0121 Primer Probe Mix O111 O121 0 O111 FAM 0121 ROX dH20 0111 Positive Control O111
11. 0121 Positive Control O121 EASY Dilution for Real Time PCR Positive Control ll 20 http wwwr takara bio coJp 3 CY238 I PCR Thermal Cycler Dice Real Time System ll with PC TP970 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Applied Biosystems 7500 Fast RealTime PCR System Life Technologies DNeasy Blood amp Tissue Kit QIAGEN 200 ll 20 10 uu IV li 2 PCR
12. 1 dH20 5 ul 1 1 2 x Cycleave Reaction Mixture 12 5 ul 1 x O111 0121 PrimeryProbe Mix 5 ul 1 Xx O111 Positive Control 1 100 0 5 ul 0121 Positive Control 1 100 0 5 ul or dH2O 5 uD dH O 1 5 ul Total 25 ul A gt DNA 3 3 3 PCR 1
13. CY238 Cycleave PCR EHEC 0111 0121 Typing Kit P9 10 P14 Lot AF4P040 AK501Z 20 P3 v201510Da 0O157 H7 026 EHEC EHEC EHEC 0 O O111 0121 O111 0 FAM 0121 O
14. http wwwr takara bio coJp 6 CY238 V 3 PCR 3 PCR 3 a Thermal Cycler Dice Real Time System TP900 Applied Biosystems 7500 Real Time PCR System Applied Biosystems 7500 Fast Real Time PCR System Life Technologies Thermal Cycler Dice Real Time System Quick Manual A lt gt PCR Hold Cycle 1 95 10 3 step PCR Cycle 45 95 5 55 10 72 20 Speed Thermal Cycler Dice Real Time System Il TP970 Normal Thermal Cycler Dice Real Time System TP900 77e TP700 Fast
15. 4 5 ROX FAM yh ts le lt A http www takara bio coJp 9 CY238 2 1 FAM ROX 2 3 14 m mm TF EU http www takara bio co jp 10 CY238 Applied Biosystems 7500 Fast Real Time PCR System Applied Biosystems 7500 Real Time PCR System Life Technologies Advanced Setup Experiment Properties Quantification Standard Curve TaqMan Re adgents PCR Hold Cycle 1 95 10 3 step PCR Cycle 45 95 5 55 10 72 25 Passive Reference none Define Tarqets Target Name 0111 Reporter FAM Quencher none Target Name 0121 Reporter ROX Quencher none Define Samples NTC
16. CY238 VII dgH2O O111 Positive Control 0121 Positive Control V O111 FAM dH20 0111 Positive Control LT rr ee O121 Positive Control ug J Mieati be es re Ci i rT Ll O121 ROX dH20 O111 Positive Control 1 Fa i i i EE 33 3 iM La La O121 Positive Control Ta i Li a 3 3 BB http wwwr takara bio coJp 16 CY238 VIIl IX Cycleave PCR EHEC O157 O26 Typing Kit CY237 Cycleave PCR EHEC O103 O145 Typing Kit CY239 Cycleave PCR EHEC O165 Typing Kit CY241 Thermal Cycler Dice Real Time System ll with PC TP970 Thermal Cy
17. 1710 2 1 1710 5 EASY Dilution 45 17100 O121 Positive Control 1 100 http wwwr takara bio coJp 5 CY238 2 1 A gt o 20 ul 1 dH2O 5 3 O111 0O121 1 2 x Cycleave Reaction Mixture 12 5 ul 1 Xx O111 0121 Primer Probe Mix 5 Ul 1 Xx or 5 ubD O111 Positive Control or O121 Positive Control P or dH20 dH 0 2 5 ul Total 25 ul lt B PCR gt a 20 ul
18. No Template Control Unknown Manual http wwwr takara bio coJp 1 1 CY238 Analysis Amplifcation Plot Threshold Baseline Manual 1 Amplification Plot Options Target O111 0121 Target Threshold Auto Auto Baseline Show Threshold Baseline rm LD get 0 WTmrwshpls aumm nnnni ne asaling mw Tmmwshna or Bair EL Gl EE Tine ci Ee En OE Tig 2 Analysis Setting Analysis Settings http www takara bio co jp 12 CY238 3 Analysis Setting Select a Target 0111 0121 Target Ct Settings for Target Name Threshold Baseline Apply Analysis
19. Settings Threshold Amplifcation Plot Log Baseline Amplification Plot ri ee EE ee TD Oa EHP CT ams Ea er liFhE hs Ia mie He Fm FL RT Ia ii pam a Hr ET ET rt ER Er LT Fh Lh FE TF FE HE PE GN PC GT Fn HT GRE NN TN CN TE Tr Ef Em TT i Cd Sh RT Era Orr TT il Ch as kor O Ti Timi hid Pasa tn a El Cm Et ti Hm Ee Mi o TF LTa ii ETC Th Te ati Wire Er Bnd Ch 1 Emm TE rE PE TE Threshold PFCmmi IO et NE 5 mm ee Ii mn 1 Baseline 1 i Oi i PP 1 TIT WF Li mn 1 FE I LT Bagla femals 1 Tarm O ThFmghgld Alm 13mD0 AUlg 33 Hg ghpmr yl ThnerEhp rl Bagglgm SE Wa Tr ERAGE Ed WI TEE StepOnePlus Real Time PCR System Life Technologies StepOnePlus Real Time PCR System ROX target ROX
20. WMH UM TL LT AA ts kl 1 Em 1 lt none gt 2 NC PC UNKN 3 A B C 1 A http wwwr takara bio coJp 8 CY238 4 W EL Em mames mm 11 AWT LM hm m EM a Lf ity a a ET 1 T Ta as amr ET TI We Jerml 1 CT LE se 1 ngl 3 Tt lt gt 1 1 FAM 2 3 Manual 40
21. cler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 EASY Dilution for Real Time PCR 9160 96well Hi Plate for Real Time NJ400 Sealing Film for Real Time Adhesive NJ501 Sealing Film for Real Time NJ500 Plate Sealing Pads 9090 48 well snap plate NJ700 Flat cap for snap plate NJ720 0 1 ml 8 strip tube amp cap Set NJ903 0 1 ml 8 strip tube individual Flat Caps NJ902 0 2 ml Hi 8 Tube NJ300 0 2 ml Hi 8 Flat Cap NJ302 0 2 ml 8 strip tube individual Flat Caps NJ600 0 1 ml 96 well plate for Real Time NJ900 gt Cycleave PCR O 157 VT gene Screening Kit Ver 2 0 CY217A B EHEC VT gene PCR Screening Set RR120A
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