1. PCR PCR JJBio21 305 0045 2 1 13 3F TEL 029 856 7527 _ FAX 029 856 7526 E mail jb info nske co jp 2. PCR PCR 1 1 DNA 2 DNA 10 20 ng 2 PCR LightCycler 480 gt Temperature Time Cycles Hot start 95C 10 min 1 95C 10 sec PCR 60C 20 sec 40 50 72C 10 sec 95C 10 sec Melting Curve UU a 1 02 C sec 95C 1 sec 3. 3 JPrnhe Melting Curves I It 3 1 QP C Melting Peaks QProbe QProbe 1 d 7 V s C a TA we 1G1 3 QP SNP m QProbe 2 QP SNP QProbe QProbe QProbe nm nm QProbe Y 520 555 QProbe R 550 580 QProbe 20 C 4. 1 DNA 5 3 PCR dNTP dUTP LightCycler480 N UNG PCR DNA 2 3 PCR LishtCycler 480 PCR QProbe 5. 0 1 0 6uM QProbe QProbe 3 0 15UM 0 5uM QProbe 0 2 0 4uM 1 2 3 4 DNA negative control DNA 6. 1 QProbe Tm PCR QProbe QProbe Tm 3 1 2 DNA negative control positive control PCR 1 2 DNA 7. QProbe SNP QP QProbe 1 SNP QProbe SNP 2 DNA SNP PCR QProbe SNP QProbe QProbe