Ultrospec 6300 pro Spectrophotometer
Contents
1.2. 9 3
3. DNA RNA DNA
4. 3 3 natural cubic
5. gn 44 Fe 3 9 1 2
6. LN 60
7. 4 9
8. print 33 Caqlibration status Fail GLP
9. 8 43 80 2106 16
10. OFF CD TEL 03 5331 9336 ss C VC CN 9 00 17 30
11. dH 10 40 3 13 kg 80 31 40 50 5 cm 90 265 V
12. j 9 AB 1 run run 1
13. BCA 1 Set up Wavelength Curve Type Regression No of stqndard 3 9 No of standard replicates 3 No of sample replicates 3 Units 1 E Bradford Lowry Biuret Interpolation a Spline
14. lt run 100 run mode
15. 13 cDNA ng pl pmol ul nucleotides dye nucleotides dye cDNA ng pl Concentration DyefrProbe DNR ngiul pmolful Nucleotides D ye 12 15 0 505 62 0 ress uny onSamos Bl15 31 con s J220 xs pmol pl 3 Other dye Cy3 Cy5 Fluorescein Set up Other dye 33 nm hmol cm Save Method
16. Tm BCA UV 50 PC Excel GLP 2 Press to reqdd
17. SE 6 Running Samples 7 run 30 function Accessory I Printer
18. GLP PRINT OUT ON GLP E TI U GLP PRINT OUT ON GLP GLP 656 nm
19. set ref load sqmple setting ref running sample SEE 6 Basic Modes Basic Modes Absorbance Transmission Concentration Absorbance run
20. ol OH T 1 1 run 2 run 0 01 99 999 F 3 3 3 Standard ml gt 0 01 99 999 1
21. 2 3 2AT 5 mm X20 mm FST 2 4 46 AL HH
22. Recall mode Methods enter 1 10 11 20 run Clear 3 mode C ethods enter User Methods Recqll Clear 1 10 11 20 P
23. 10 50 mm 80 2109 70 35 80 2106 04 80 2105 49 3 80 2108 01 8 36
24. 17 Graph Max Abs Min Abs 4 Standards Sz Autoscale Y axis post run Regression UV methods lm Dj D 3 1 Protein Impurity 280 nm Yes SE Curve Type
25. NADH LDH NADH H NAD NADH 340 nm LDH 80 340 nm Abs 340 A B C D Time 3 A B B C C D 1 0
26. Run set ref 10 Turning lump on 15 Ou 4 mode function set ref print ec ge 4 PP
27. H DNA RNA Oligo DNA RNA Oli 15 Tmf 0 1 1 2 5 go 1 run lt gt
28. 2 2 Standard Curve 1 Set up Wavelength Kinetics Curve Type No of Standards 3 9 No of Sqmple Replicates 3 No of Standard Replicates 3 Units 2
29. GE SEE 169 0073 0 3 25 1 Ss ISO 9001 2000 Re 5 an Was FE TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com Home Page www gehealthcare co jp lifesciences GE Heqlthcare Fr
30. 1 0 000 0 000 3 4 2 Timing Time units Delay 0 1000 Reaction time 3 Concs Sqve Method mode conc 29 4 Graph LCD 8 Maximum Abs Minimum Abs Autoscqle Y axis post run Yes Y 5 Standards
31. EH 80 2106 05 80 2107 14 80 2106 06 8 10 mm 10 50 mm 100 mm 5 ul 80 2103 68 70 pl 80 2103 69 37 80 2106 09 H 80 2106 10 80 2106 08 80 2106 11 80 2106 13 50ul 80 2076 38 100 mm
32. 7 Concentration Factor Standard 2 1 3 Factor 8 3 a Faqctor stqndard 0 0 1
33. 9 10 11 12 13 9 45 15 CD 750 1
34. on 2 nm Dota Intervql Units 2 8 8 21 enter run Ratio Concentration Sqmple 1
35. 1 Instrument Reqdy Cr 5 31
36. 1 Set up Stort 190 1090 nm End 200 1100 nm Scan Speed Data Intervql Reference scan Sqve Method enter nm min E 8 E run Fast Medium Slow 2 1 0 5 0 2 0 1 nm ast min om zo io eo edum wan za00 io eo 300 150 100 300 200 10 lt 500 nm 0 1 nm
37. 4 Results cDNA Abszeo Abszso 1 5 260 nm cDNA cDNA Absz Abszso Abszso Abszeo pmol ul Abszso Abszao ng 14 Tm Calculation Tm Tm
38. 15 Protein 3 U 3 4 EE UV Bradford BSA
39. Ew enter GLP CALIBRATION FAIL GLP GLP E a GLP Enabled E 3 F 10 8 h Stop3 LCD 49 GLP Enabled UItrospec 6300pro UV Vis Spectrophotometer Lab name Instrument Serial no 81012 Softwore 4197 V1 4 Slave 4194 V1 0 Last serviced 14 0
40. 750 nm 3 5 100 ug 3 3 546 nm 3 9 562 nm 1 2 000 ug ml UV UV 205 280 3 215 225 nm Information 16 Bradford Lowry Biuret BCA
41. GLP GLP CALIBRATION FAIL enter 35 2 EH il E
42. 9 print yes no 3 9 0 del 33 MA cDNA 50 3 CDNA in situ labelling gt 3 PCR lt 4 rm DNA RNA 10 mm 260 nm 1 0 50 40 hg ml 33 hg ml
43. 2 Multiwqve 21 1 2 57 nm 350 Application HH 260 280 nm 80 2103 68 320 nm 10 mm 2 2 5 mm
44. 8 Zeitung 310 384 1941 260 nm mg ml mg ml F 280 Abs a 280 nm 3 Christian Warberg Biochemisceh 1 55X Abs2 gt so 0 76X Abszeo 1XAbszso 2XAbsz o 280 nm
45. 4 Of 1st 2nd 4th Derivative Smooth Enhance Transmission 4 Scale Offset 4 Peak Table on lll 15 nm 4 Multiwave 1 Abs Ratio Absorbqnce Ratio L 9 2 2 AB Factor 1
46. Abszs 260 nm X 260 280 DNA RNA 1 8 2 0 230 nm 230 nm Tris EDTA RNA 260 230 gt 2 0 RNA 230 260 nm
47. 2 Concs 1 Integration Time Save Method run 2 3 0 1 1 2 5 3 0 000 1 RI run mode 9 concs
48. cDNA cDNA 2 CDNA oN Cy3 Cy5 in su 1 Set up Cy3 Cy5 Fluorescein Other dye Other dye Other dye
49. Set up Integration Time 0 1 1 2 5 Sqve Method Yes enter Run run mode 3 Graph Maximum Abs Minimum Abs conc Autoscale Y axis post run Yes Y 4 Standards SE
50. enter 1 Set up nr EE A1 A1 Integration time Default 2 Factors 1 2 enter 2 2 3 Equation 1 3 enter C Enter Stop 4 ECA NI Equation enter C Enable equation Save Method Equation 2 24 Kinetics 9 1 Set up Wovelength E 6 Ou 1
51. PCR Breslquer et al Proc Natl Acad Sci USA 83 3746 1986 DNA RNA RNA T U ug ml ng ul mer 10 mer pmol pl pg ml Tm C Info
52. gt Factor Units Auto set ref 4 yes enterd KR 1 U 2 Timing Mode Serial Parallel Paraqllel Serial TimeUnits Deloy Parallel gt 0 1000 Reaction time 600 Parqllel Interval ii Sqve Method Yes enter
53. GLP PC PC 31 9 Auto print PC Auto print print PC 33 2 Baseline View New Sqve Restore View
54. 51 Beer dA dt dC dt dA dt x 1 7 EL dC dt mol L 1 cm E NADH E 6300 Imolcm dC dt x Vt Vs Vt Vs 2 1 U U 25C 1 1 2 kat 1 1 1IU 1 67 x 10 kat 1kat 6 x 107IU S
55. 80 2110 73 eo7r67 tnt 72 0496 01 Seiko DPU 414 71 1600 04 N 80 2103 68 5 HI 80 2103 69 70 40 SWIFT II SWIFT Tm 8 SWIFT ll METHOD SWIFTI LAB 3 SWIFT Il SPECIALIST 3 TT SFIFTII METHOD 80 2108 31 SFIFT II LAB 80 2108 26 SFIFT Il SPECIALST 80 2111 91
56. CN 3 9 wn 6 7
57. mol IU I kaqtl 01 dA dt IU I Factor pmol l Vtx 10 EXLxVs Vt ml Vs ml E I mol cm L 1cm 0 2 ml 2 20 ml Factor 2 20X10 6300X1X0 2 1746 umol l 52 dA dt vs
58. 1 X Abszso Abs szo 2 X Abs ss AbS 320 320 BB 280 2 0 00 BSA bovine serum qlbumin H 1 1 115 0 0 8 mg ml 18 1 1 55 mg ml 2 0 76 mg ml enter run
59. IU katal 60 IU dA dt X n gt x gt y nzxy ZXxZXx nZX2 _zy lt X 1 Kinetics Std Curve ZX2Zy nzXxy ZxX 2 nzZx2 Zy 2 nzZy2 100 x 53 595 546 562
60. 4 1 Set up Wavelength Curve Type Regression Interpolation Spline Factor Factor Factor Number of standards 3 9 Factor Number of standard replicates 3 Number of sample replicates 3 Units Unit 2 lt 1 3 4 0 000 8 0 000 s 27 2 Concs
61. New Sqve Restore 3 Clock CD 4 Service 77 1 2 2 qg b c 2 A B C
62. cDNA 260 nm FISH 14 12 Cy3 Cy5 430 nm 400 nm 410 nm CDNA RNA ng X 99 999 RNA RNA 11 Scan 12 260 nm
63. 200 lt 350 nm F ADbSsszeo AbSszso H 1 cDNA PCR 850 nm 260 280 nm 320 nm 10 Abszeo Absszo X Abszso Absszo Abszso Absazo 260 280 320 nm
64. Worburg Christign mg ml 1 55 x Abs zao 0 76 X Abs zeo nm 260 nm 280 X Y 260 nm X Warburg and Christian Biochemisches Zeitung 310 384 1941
65. Tm Tm 8 3 PC 5 IBM 486 OS Microsoft Windows 95 98 XP NT 2000 RAM 8MB 500 MB 3 5 CD ROM COMMS 1 Tm SWIFT Tm VGA ou 1 E 41 21 CFR part11 compliant SWIFT II
66. 1 2 Empirical 280 205 nm Method mg ml mg ml 27 120 Aso A2o Scopes R K Anal Biochem 59 277 282 1974 enter run Ratio Concentration 3 Empirical 215 225 nm Method ug ml mg ml 144 xX A215 A225 Waddel W J J Lab Clin Med 48 311 314 1956 enter
67. 10 Parallel gt Function 3 No of samples Active reference 8 Active Reference 7 ot Serigal run SOUND ON Set up user SOUND OFF T SlopeXFactor 5 Parallel run
68. 0 2 nm 10 250 nm SWIFT II 20 2 Graph 4 3 4 8 9 enter 4 3 3 Overlay
69. enter mode 4 a function set ref print PC print 8 Aut0O 32 enter
70. run3 Ratio Concentration Info Aszso mg ml 19 Applications Wavescan 3 1
71. 2 ot 4 3 Equation 1 and Equation 2 Description Equation Units Unit _ WWW BB 2 Enable Equation enter run Equation 1 Equation 2 23 Multiwave Cobalt g l 2 26 xAsr 0 30xAzzo X100 Equation 1 Nickel g l 0 40 xAs 27 41xAzzo X100 Equation 2
72. 3 E 320 nm 1 54 set up 2 260 280 nm 2 260 nm 0 280 nm 2 0 1 BSA 1 1 115 0 lt
73. 10 40 mm H 8 H 2 5 mm 20 49 2 38 80 2106 12 25 30 37 2 Tm 80 2106 14 SWIFT Tm 20 105C DNA RNA
74. 47 Pharmacopoeia Good Laboratory Practice GLP 0 02 v v 20 1 269 nm 266 nm 1 mm 2 0 2 1 The European Pharmacopoeia 1984 V 6 19 2nd Edition
75. oO 2 Abs Diff Absorbance Difference 1 1 2 2 AB Factor1 1 Factor2 2 Units 2 8 enter E run 1 3 3 D 3 point net amniotic d Result 9 Sample Oint net 1 2
76. 546 595 nm 2 000 3 0 0 1 2 0 0 2 6 0 95 1 00 UV 280 nm 3 2Z2 nm
77. U V RADIATION HOT N WARNING U V RADIATION IS HARMFUL TO YOUR EYES 3 LCD 47
78. 3 gt 1 10 ug 5 Lowry H 595 nm 3 10 100 ug E Folin Ciocalteu BSA 1 20 ug Biuret i 3 5 130 mg ml 8 BCA BCA BCA bicinchoninic acid BCA UV methods
79. set ref 9 33 ug m run 9 11 Scan O 200 350 nm RNA 200 nm 3 350 600 nm UV 5 1 1 2 2 70 HI 80 3000 81 750 HI 80 3000 77 200nm lt 220 nm
80. 1 48 OK ibration status visible only ibration status UV only 43 o iled to qlign visible lamp Abs Non Linegr 4
81. 80 2105 49 PC 1 lt 39 FW WW 80 2112 15 80 2106 05 80 2106 06 80 2106 07 80 2106 12 80 2106 13 80 2080 60 80 2105 49 6 80 2106 04 Tm 80 2106 14 80 2112 13 71 0496 01 80 2106 19 CS 80 2080 74 80 2080 60
82. C stop 4 bYW enter stop 32 Auto print Set up print print PC PC 80 2110 73 80 2105 97 Excel
83. 8 C run stop 5 2 q DNA RNA Oligo UV b
84. cDNA T 2 Scan on gt slow medium fqst y medium slow medium cDNA 3 260 nm Cy3 Cy5 488 550 650 nm 2 260 nm
85. Multiwqve 9 9 2 CE ET EEE 4 1 Set up No of A s 9 1 1 Integration Time 0 1 1 2 5 4 2 Factors Dilition Factor C K1 1 C
86. run 2 run 3 run B Methods mode 3 Save 33 Save 50 save 1 10 11 20
87. 3 8 E Factor Units 1 2 3 bilirubin 3 2 8 ca enter DR 22 9 run Height Areq Saqmple 8 1 4 MultiWave
88. 0 000 press to reqd run mode fill TT Transmission
89. 1 2 wy 1 cm 2 000 Pharmacopoeia Instrument Qualifcqtion and PerformaqnceVerifcation Logbook CD ROM CD ROM 48 Good Laboratory Practice Good Laboratory Practice E il F
90. Seiko DPU 414 Displaqy pp high contrast GLP GLP 48 gil GLP Set up 1 User GLP enter GLP
91. 5 Running Samples run G 28 F 10 LP NS IND
92. 21 CFR part11 compliant SWIFT ll FDA 21 CFR Part11 SWIFT Il FDA 21 CFR Part11 PC1 CFR part11 compliant SWIFT II 80 2117 57 Tm CFR 9 PC 21 CFR pqrt11 compliant SWIFT II Microsoft Windows OS NT 4 0 with
93. Too much light Beam blocked IE lt lofip cus 34 Function Accessory
94. AA o Post run 25 3 Graph Moaximum Abs Minimum Abs Autoscale Y axis Y Show Data Points Plot graph when printing results 4 Post Run Parallel Sqmple number Results Initial Abs Final Abs Slope Slope X Factor Result Linearity Stqrt time Auto find end points End time Yes 3 No 26 3 UL H Std Curve
95. Service Pack 6 2000 with Service Pack 2 or 3 XP with Service Pack 1 PC 1 NTFS New echnology Filing System FAT HPFS Ql 42 GLP GMP 1 ISO 9001
96. at 220 nm using Nal and lt 0 025 T at 340 nm using NaNO gt 9 pin serial and centronics parallel 210X140x80 mm 520x370x230 mm 13 kg 90 265 V AC 50 60 Hz 150 VA EN 61010 1 EN 61326 2 3 Generic emissions EN 61000 4 6 Generic immunity part 1 EN 61000 3 2 Designed and manufactured in accordance with an ISO 9001 approved quality system 207049 56 12 Biochrom Ltd 22 Cambridge Science Park Milton Road Cambridge CB4 0FJ UK 5 2007 GE
97. nm BSA BdH 595 nm BCA 562 nm bicinchoninic acid BCA 546 nm BCA
98. press to reqd TR TS TV 15 Turning lamp on 2 FX 0 N WARNING
99. 0 8 mg ml mg ml 1 115 Xx Absza Abszso HiTrap 55 Safety standard EMC emissions EMC immunity Mains harmonics Quality System British design registration No 190 1 100 nm in 0 1 nm data intervals 1 200 lines mm aberration corrected concave grating 4 000 nm min at 2 nm intervals 1 0 nm 0 5 nm 0 2 nm Tungsten halogen and duterium lamps Silicon photodiode 3 000 3 000 A 99999 99999 concentration units 0 1 T 200 T 0 5 or 0 003 A to 3 000 A at 546 nm whichever is the larger Within 0 5 of absorbance value to 3 000 A at 546 nm 0 001 A per hour at 340 nm at 0 A after warm up duterium lamp lt 0 0259 T
100. 8 00 13 36 Instrument state at calibration GLP Caqlibrated 09 10 00 at 12 56 Calibration Full UV Visible Bandwidth 1 3 1 8nm 1 7nm PASS Woavelength 656 1nm 656 0nm PASS Absorbance at 220nm 1 763 1 781AJ 1 772A PASS 340nm 1 633 1 665Aj 1 649A PASS 500nm 1 477 1 491Aj 1 484A PASS Stray light at 220nm lt 0 025 T 0 013 T PASS UV lamp 85 of original energy Vis lgmp 95 of original energy Current instrument state Accessory Eight Position Cell Changer UV lamp installed 14 08 00 13 35 use 5 hours baseline in use 14 08 00 13 38 baseline stored 14 08 00 13 38 Vis lamp installed 14 08 00 13 35 use 5 hours baseline in use 14 08 00 13 38 baseline stored 14 08 00 13 38 GLP Enqbled 50 Reaction Kinetics ALT og
101. GE Healthcare Ultrospec 6300 pro GE imagination qt work Contents ss sse sssss senses sses sssssssssssssssssssssss 2 nN kk 3 4 4 5 yw 82 5 BasiGModes ti 7 EN 9 Nucleic uk 10 Protein LLLLELLE 16 J ES EE 3 2 33 BGA 33 RR No NN 34 eNONes 35 35 ER 37 2 40 SWIEEI LE 41 21 CFR part11 complignt SWIFTII 42 43 43 ER 44 i_ LL 47 48 GRGGBOSIO 48 G69GdiILGDOEGtOIViBEOGH6BIL200AAnn 49 54 9080RC8036325022820005288R2R28 2227260020208R 56
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