TaKaRa ノロウイルスGI/GII検出キット
Contents
1. 3 1 2 3 PCR PCR2. 1105001 25 10 22 1022 1 RT PCR RNA One Step RT PCR PCR TaqMan 1 Cl GII Gl GII RT FAM ROX Cyanine5 1 TTTT 2
3. NL i 3 ww efs N 1 TaqMan http www takara bio co jp 2 RR255A 100 1 Lysis Buffer NV 1mlx 2 2 PCR Buffer NV 1 950 ulX 2 3 Enzyme Mix NV 2 100 wl 4 NVPrimer Probe Mix 3 250 ul Oe 5 NVPositive Control DNA 50 ul 20 1 dNTP Mixture Mg 2 PrimeScript RTase RNase Inhibitor 7242 Ex 7ag HS 3 ll 20 200 l 20 ll 10 ul 90 5 PCR LAM ROX Cy5 Thermal Cycle
4. 2 10 000 15 000 rpm 5 http www takara bio coJp 4 RR255A 2 1 Lysis Buffer 5 Ul Lysis Buffer NV 20 ul Total 25 ul 2 90 5 3 RT PCR 1 1 go PCR 22 5 ul 2 1 PCR Buffer NV 190 Enzyme Mix NV 1 0 ul NVPrimer Probe Mix 2 5 Total 22 5 ul 2 NV Positive Control DNA 2 5 ul 3 3 Thermal Cycler Dice Real Time System Applied Biosystems 7500 Fast Real Time PCR System
5. 121 20 http www takara bio coJp 3 RR255A V VI Enzyme Mix NV 50
6. PCR PCR Manual LR PBS RT PCR NV Positive Control DNA 2 1 PBS 5 10 PBS
7. Gll GI FAM Gl ROX G FAM GI ROX nt http www takara bio co jp 7 RR255A VIIl Thermal Cycler Dice Real Time System Ill Cy5 with PC TP990 0 1 ml 8 strip tube individual Flat Caps NJ902 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 Filter Unit Cy5 for Thermal Cycler Dice Real Time System TP803 Filter Unit Cy5 for LED TP703 0 2 ml 8 strip tube individual Flat Caps NJ600 TaKaRa qPCR Norovirus GI GII Typing Kit RR250A B IX
8. lt gt 42 C 5 gt 95 30 lt PCR 40 gt 95 5 56 C 1 FAM ROX Cy5 Thermal Cycler Dice Real Time System Il TP990 Speed Normal Thermal Cycler Dice Real Time System TP900 77e TP700 Speed Fast CFX96 Touch ReaLTime PCR Detection System gt 42 5 gt 95 30 lt PCR 40 gt 95 5 56 C 1 20 FAM ROX Cy5 http www takara bio coJp 5 RR255A 4 I I GI FAM ROX IC Cy5 eeAw cuoo GD a G
9. Thermal Cycler Dice 7aKaRa 797 TaqMan Roche Diagnostics GmbH PrimeScript Idf4R4 Tel 077 565 6999 Fax 07 565 6995 http www takara bio coJjp v201510Da
10. gs RR255A IdRaRa TaKaRa GI GII v201510Da 37 38 1 RNA PCR PCR Genogroup gt V 5 Genogroup GI II GII PCR GI GII GI ll
11. l GI PCR Gl Gll 0 em EE ES ECT T G GII IC Wefe Gl Gll IC IC LUF IC PCR RNA http www takara bio coJp 6 RR255A VII GI FAM Gll ROX IC Cy5 1 GI FAM GI GICFAM GII ROX
12. r Dice Real Time System Ill Cy5 with PC TP990 Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Lite TP700 TP760 TP900 TP960 TP700 TP760 Cy5 Filter Unit Cy5 for Thermal Cycler Dice Real Time System TP803 Filter Unit Cy5 for LED TP703 CFX96 Touch Real Time PCR Detection System BIO RAD Applied Biosystems 7500 Fast Rea Time PCR System Life Technologies Applied Biosystems StepOnePlus RealTime PCR System IV 1 2 3
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