IO テスト FITC 標識抗体 CD5 IOT1a 形状・構造等（キットの構成） 操作
Contents
1. 2 3 4 5 6 7 8 CD5 IOT1a A08932 100 2mL 1 Reinherz EL et al2. a 2 a 2 VS FITC LumphocutesS Leukocytes Fk 1 10 i100 1 B FITC CYTO TROL 6604248 Fc IO Test CD45 FITC CD14 PE A07738 I IM1201 Fc
3. EDTA 6
4. PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 59 0 1 PBS VersaLyse PBS 1mL IOTest 3 A07800 I IM3515 12 5L 0 19 PBS VersaLyse PBS 1mL IOTest 3 A07800 IM3515 67 5uL 0 5 PBS A09777 IM3648 VersaLyse IM1400 OptiLyse B A11895 A11894 OptiLyse C
5. 5 PBS 5 10 x10 mm 1 12mm x 75mm 2 100L 3 20wL IOTest lgG1 FITC 20L 4 15 5 6 PBS 2mL 7 400 xg 3 8 7 8 9 0 5 1mL 0 19 0 59 PBS 10
6. 24 3 Ficoll Paque 1 PBS L 1 30 8 400 x g 8 PBS 8C 400 x 9 4 4 9 2 1 10 PBS 5x 10 mm 5 x 10 mL 11 90 1 12mm x 75mm 2 Ficoll Paque 5x10 5 x
7. IO Test T B NE
8. A07799 I IM3514 IOTest 3 Hn Bn Bn dh dh IO Test lgG2a FITC A12689 I A12690 100 2mL Ficoll Paque Phamacia 17 0840 03 2 1 100 L 5x 10 mm 10 x10 mm PBS 3x 10 mm 1 25C 500 x g 5 2 3 4 LH700
9. 2005 10 A08932 2004 10 20200EZY00112000 T IO FITC CD5 IOT1a 1 2 3 4 1 IO FITC CD5 IOT1a FITC IgG 20L 50 ux g mL IO FITC FITC Fluorescei cyanate 100L 1 SI
10. IO CD5 HPB ALL 1 JURKAT 2 95 CD5 KM3 3 0 5 1 HPB ALL T cell Leukemia 2 JURKAT T cell Leukemia 3 KM3 Non T Non B Leukemia PBS 4 CD5 HPB ALL JURKAT 3 10 3 5 5 E AH 1 0 1
11. CD5 67kD CD5 T B B CLL CD5 B BB CD72 T Fluorescein isSothiocyanate 485 509nm 504 541nm CD5 CD5 CD5 FITC lol ri 4 6 FR 10 11 IO
12. 22 Nadler LM Anderson KC et al 198 244 250 23 Polk B Fox R et al 1987 New Engl JJMed 316 61 66 24 Rebai N Maissen B et al 1982 Eur J Immunol 13 106 111 25 Reinherz EL and Schlossman SF Cell 19 821 827 26 Reynolds CW and do JR 1987 Immunology Today 8 172 174 27 Rouas Freiss oc Natl Acad Sci USA 94 mmunol 15 88 91 J Immunol 135 973 979 a HI et al 1984 Cell Diff 14 223 230 al 1991 Eur J Immunol 1981 11 18 M et al 1991 Immunology 72 150 157 et al 1983 New Engl J Med 309 1275 1281 987 Immunology Today 8 308 315 105 0001 3 5 1 di IMMUNOTECH A EE RMAAN COULTER COMPANY 130 avenue de Lattre de Tassigny B P 177 13276 Marseille Cedex 9 France 105 0001 3 5 1 oe COULTER
13. ed 1986 Leukocyte a Typing I Vol 1 SDrin9er Verlad pp 303 313 2 McMichael AJ ed 1987 Leukocyte a Typing I Oxford University Press pp 144 147 210 214 234 238 239 241 480 484 579 581 889 895 3 Knapp W et al ed 1989 Leukocyte a Typing IV Oxford University Press pp 36 38 274 277 295 342 343 4 Schlossman SF et al ed 1995 Leukocyte a Typing V Oxford University PresSs pp 507 509 515 518 790 794 795 798 1503 1504 5 Beverly PCL and Callard RE 1981 Eur J Immunol 11 329 334 6 Bryan CF Newman JT et al 1987 Trans Proc 19 4340 4344 7 Clark P Normansell DE et al 1987 Blood 67 1600 1606 8 Clevers H Dunlap S and Terhost C 1988 Eur J Immunol 18 705 710 9 Denning SM et al 1988 J Immunol 141 2980 2985 10 Freedman AS and Nadler LM 1987 Semin Oncol 14 193 212 11 Foon KA and Todd RF 1986 Blood 68 1 31 12 Hannet l et al 1992 Immunology Today 13 215 218 13 Haziot A et al 1993 J Immunol 150 5556 5565 14 Keizer G et al 1985 Eur J Immunol 15 1142 15 Kishimoto TK et al 1990 Proc Natl Acad Sci USA 87 2244 2248 16 Koepke JA and Landay AL 1989 Clin Immunol Immunopathol 52 19 27 17 Kung P Goldstein G et al 1979 Science 206 18 Laffon A et al 1991 J Clin Invest 88 546 552 19 Lanier LL Le AM et al 1983 J Immunol 20 Lum LG 1987 Blood 69 369 380 21 Maissen B Rebai N et al 1982 Eu
14. 10 mm 100 L 3 20 L IO Test lgG1 FITC 20L 4 2 8 30 5 PBS 2mL 2 8 400 x g 5 6 5 7 0 5 1mL 0 59 PBS 8 24 FS 90
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