抗体精製用レジン - プロテイン・エクスプレス
Contents
1. 732 6204 3 1 PBS PBS 0 1 M Glysine pH2 5 Bipo Resin ProteinA Weak Acid 0 1M Citrate pH3 5 4 0 1 0 M Tris pH9 0 2 500uL 100uL 3 000 x g 1 400uL 2 PBS 400uL 2 3 3 1 5 Resins f2. scFv SDS PAGE IgG scFv SDS PAGE 1 2 3 4 5 6 7 8 9 10 11 12 18 1 marker IgG from Bipo Resin Protein A Alkaline Resistance IgG from Bipo Resin Protein A Weak Acid IgG from competitor ProtennA Resin marker IgG from Bipo Resin Protein L Alkaline Resistance IgG from competitor ProtennL Resin 8 scHv from Bipo Resin Protein L Alkaline Resistance 9 scEv from compettor ProtennL Resnn 10 marker 11 IgG from Bipo Resin Protein G Alkaline Resistance 12 lgG from competitor ProteinG Resin 13 marker 3 20 0 ES 2 9 2 CIP CIP 10096 Bipo Resin Protein A Alkaline Resistance 1M NaOH 10 Bipo Resin Protein L Alkaline Resistance 15 mM NaOH 10 Bipo Resin Protein G Alkaline Resistance 50 mM NaOH 10 1 YNS 1 CO CO CO 1 U U 1 U lt e Bipo Resin ProteinG AR
3. 20 Bipo Resin ProteinA AR Bipo Resin ProteinL AR competitor A 20 40 60 80 0 10 20 CIP cycle CIP cycle CIP cycle Competitor A Competitor A ee O 1 E m lt Residual Binding Capacity Residual Binding Capacity 9 6 Resins for antibody purification 3 Bipo Resin Protein A Weak Acid pH4 5 Bipo Resin Protein A Weak Acidg BB A IgG pH4 5 IgG 100 80 60 40 IgG Recovery pH4 5 20 Bipo Resin ProteinA WA competitor B 7 Protein A i code AAR P Alkaline Resistance Protein A code AWA P Weak Acid Protein L code LAR P Alkaline Resistance Protein G code GAR P Alkaline Resistance 8 23 26 Resins for antibody purification ee 9
4. URL http www proteinexpresS COJD 260 0856 1 8 15 TEL 043 202 5755 FAX 043 202 5756 E mail service proteinexpresS COJD
5. IgG L x Fab fragment antigen binding scFv single chain variable fragment mg mL resin Bipo Resin Protein A 28 human IgG Alkaline Resistance Bipo Resin Protein A 31 human IgG Weak Acid Bipo Resin Protein L 13 human 1gG Alkaline Resistance 1 2 scFV Bipo Resin Protein G 26 human IgG Alkaline Resista
6. lt ProteinExpress Version 1 1 Bipo Resin Protenn A Codej AAR 025 Alkaline Resistance Bipo Resin Protein A Code AWA 025 Weak Acid Bipo Resin Protein L Code LAR 025 Alkaline Resistance Bipo Resin Protein G Code GAR 025 Alkaline Resistance URL httD www proteinexDreSsS CO D 260 0856 1 8 15 TEL 043 202 5755 FAX 043 202 5756 E mail service proteinexDreSsS CO D Resins for antibody purification 1 Staphylococcus aureus A fFinegoldia magna Peptostreptococcus magnus L group G streptococci G A G lgG
7. nce 1 Bipo Resin Protein A Alkaline Resistance Bipo Resin Protein G Alkaline Resistance Bipo Resin Protein L Alkaline Resistance A L G L G Resins for antibody purification Bipo Resin Protein A Weak Acid pH3 5 4 5 A 2 Fab scFv 25mL resin in 20 EtOH 50 slurry 60um 4C
8. or antibody purification 3 2 3 3 PBS 400uL 3 3 4 100uL 1 lt 2 5uL 2 4 Bipo Resin Protein A AIkaline Resistance 100 mM NaOH 10 Bipo Resin Protein A Weak Acid 100 mM NaOH 10 Bipo Resin Protein L Alkaline Resistance 15 mM NaOH 10 Bipo Resin Protein G Alkaline Resistance 50 mM NaOH 10 20 4 C 5 ELISA Resins for antibody purification 6 1
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