(57)【要約】 （修正有） 【課題】メラニン前駆体の効率的な製造方法の提供
Contents
1. 0 10000000000000 10000 001000 210002. oo 8 5 ZE _ 6 oono amp 5 _ 35 C O
3. 0 1000000000 5 00000 0 1 2 1000000000 301 40 0 50 2100000000000 100 2 80 3 2 100 200 200mM 0 0 pH 3 40 0 50 11000000000 L DOPAJ 0 0 1 0 0000000 0 5 10 10000000 00010 0 00000 50 OD L DOPAJ 000000 x 10 1100000000 10000000000 0 0 L DOPA 1 900000000000000 5 10 0
4. N 7 O O O lt 5 O lt Epi 2
5. 17 2006 158304 2006 6 22 100 5 6 0 0 5 6 2 0 0 5 6 0 0D 0 0 5 6 3 20 5 6 5 6 0 2 0 100 0 11 1000000000000000 2002 1913660 0 0 0 0 0 1000000000000 conp 00000000 Aspergillus oryzae 5 10138 80 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 FERM 1652808 1 590 10000000000000000000 1 5 240 g0
6. 130 0 140 5 1000
7. O 5 3 ILJ lt _ 5 05 5 lt 5 g 1 g 1 2 2 5 4 nN O 5 5 oon 3
8. poog 0 0 L DOPA 00 10 103 0 L DOPA 1191000000 100000000000000000000000 5 10 u 10000000 000 0 2 80 3 200000000000000000000000000000 0 0000000000000000000 0 Aters HPLC 2695 2996 000000000000000 makt Uni son UK 18 4 150mm 100000 1 5 0 99 9 060000 B80000000 B0 0 0 0 0 50 0 0 50 100000000 1 0 0
9. 1995 14 1261 1 151 154 0 0 000000000000 600 20 6 0 1000 10000000000000000000000000 0 00000000 000 000000 000000000060 000 0000000000 00000 30 000 100 0000 100 100 40 0000000000 1000000000000 1000000000000 0000000000000 2 81 3 20 0 000000 000 00000 1000000000000 1000000000000000000000000000000000 50 9 2006 158304 2006 6 22
10. N lt _ _ _ 25 oono 5 go O O g o g g o O g 2 O mm o o O 25 2200 o c oono g O g Oo O TS O O o g N
11. 10 2006 158304 2006 6 22 1000000 177000000 000000 taci 0 0000 0 5 0 5 0 0 T70 0 taco 0 00000 10000170 58001 000000 0 0 0 O0 000 0 0 0 0 O0 O O PHOSO 000000 4140 0000006 AL10 CUP 10000000000000000 000000 AD H1 0000000000 0 800 000000 91 gt 4500 0000000000 0 2001 2243811000 37 25kDa 0 000 0 0 0 0 2651 10 0 0 44 5 18 00 100 000000000 0 0 0 DNAD 52 P1000 0000000000 reese
12. O O O O O O 0 1 O 1 Vo O O O C N
13. 13 2006 158304 2006 6 22 1 1 N 1 1 800 3001 109 100000 5 6 oOo a aA
14. 0000 L L L DOPA L 10 L 000 00000000000 ooog en ama 5 6 40 40 20 000 NH H COOH DOPA PCR Sma H ligation N N N HO 5 6 2 YKL 56 Stu gt lt 2 DNA 0000 1200 6 5 1000 8 800 43 600 27 400 1 200 0 0 0 0 20 3 40 ahii aa oN owo 20 HAED 5 1 5 5 0 10 20 30 2 1000
15. 5 1000000000000000000 100000000000000 5 6 5 6 10 2 0800 000000000000 1000 10000 10000 000 000 10000 N 5
16. 8 gt w 6001 1 90 4 4 L 0 0000000000 0 0000 0000000000 0 000 L 100 21 00 7 90 1 0 03 SO 500 2N K 0000000 0 5 50 L 10000 1 35 127 0140 100
17. 8 2006 158304 2006 6 22 10 2002 1913660 DJ 2002 373069 Mol ar cloni ng sequence of the 5 gene from Aspergillus oryzae expression of yeast 15 5 Act
18. 00000 00000 10 5001 1 0000 0 0 0 50 200 1 DO00000000000000000000000000000000 100000000 O 20 oOo 200 30 1000 00000 2006 158304 2006 6 22 14 10 10
19. Peni ci 1000000000 Aspergillus oryzae 0 000000 8 9 900 1001000 Escherichia 3 2006 158304 2006 6 22 g m
20. 2 12 E 101 0 11 13 8 2 gt 14 0010 1000000000000000000000 130 15 1000000000000000000000000000000000 W 16 10000000000000000000000000 2006 158304 2006 6 22 7
21. 5 1 010190 8001 000000 00000000000 5 000000 lt 10 20 30 40 50 18 2006 158304 2006 6 22 N O pH3 0 w gt
22. _ H N 5 5 o _ 2 N lt 6 ooo gt o0 g O 2o o mm lt 5 o _ 7 1 l O Tn _ l o0 go mm mm 22 21 5 Era A 22 OA 7 o0 g o o lt 22222 z oonan A N 2 oo 1
23. 2006 158304 2006 6 22 19
24. 2006 158304 2006 6 22 19 3982006 158304 2006 158304 43 8 22 2006 6 22 51 Int Cl 12 13 0 2006 01 12 13 0 ZNA 48024 12 15 09 2006 01 12 15 00 48064 20 OL 26 R 21 4 82004 355179 2004 355179 71 000165251 22 16 12 8 2004 12 8 2 4 7 74 100065215 74 38 100076510 Aat 74 IREA 100118382 RF 72 1 24 RRR SMAA 72 REA 24 RER EMAA lt 54 5 AREORA 57 000 10000000000000 000 99000000000000000 1000000 00000000000000 0 0 00000
25. 100000000000000 100000000000000 000
26. 2006 158304 2006 6 22 10 20 30 50 2006 158304 2006 6 22 5
27. 1 12 0 d9 0 0000 10000000 d6 0 00000 9 99 00 0000000 0 0 2002 320477 2003 61659 2003 1642831 0100000000000 0 sodM gl 0 sodM DOPAD 000 0000 0000 0000 L L DOPAD L 0 10 20 30 50 2006 158304 2006 6 22 11
28. m oo EA gt 5 _ gt 5 gt 5 N 5 l l 2 5 2006 158304 2006 6 22 20
29. N 25 27 5 e ____ Eps 7 1 _ H 5 g 2 o 2 o i _ E o 5 5 5 _ 2 _ 2 LO _ _ 5 _ 22 gt 6 2006 158304 2006 6 22 1000000000 100000 1000000000000000 Aspergillus 5 10000 Rhi zomucor 1000000000
30. _ O o O O 7 Q O O o O o O O A Ng i z O 5 c O EN O N EE lt O O E O o o N mm o go 1721 o o g lt O O O 2 5 2006 158304 2006 6 22
31. 2 50 2 350000 300000 250000 200000 150000 100000 27 50000 0 0 15 30 45 99 50 PERZIJE 350000 300000 250000 200000 gt 150000 2 57 50 2 2 1 400000 300000 200000 100000 30 4 2 2006 158304 2006 6 22 5 2 558 4 4 Po 777 0 400000 800000 1200000 HPLC 6 7 IU 7 280 200000 4 16 2 180000 160000 140000 2 8 120000 100000 H 80 80000 U 60000 40000 20000 o gt 4 4 4 5 5 2 5 88 x 55 2 57 4 s g E E R 25 2006 158304 2006 6 22 _ 400000 250000 350000 _ 190000 5 250000 100000 8 00600 50000 56 o DHble W 150000 2 15 30 45 100000 99 50000 o 25
32. 9001000 0000000000000 001000 40 10000000000 5 6 10 0 40 C 5 6 2 0 0 10000000000 0001000 100010100 L 10001000 5 10 0 100 0000000000000 0 1000000000 10 20 30 50 16 2006 158304 2006 6 22
33. 5 T T x E Zi 0 gt 7 ELE h non g 5 _ 2 N _ 5 z 5 28 1 _ 22 5 oo g ooo oono oo lt o e O i 1 oog
34. 0 5 6 2 000000 O 5 6 2 5 6 0 0 1000 2 0 00000000 2 5 1000000000000000 45 5 6 2 0000000000 0000000000000 140 0 0 000000 2 0 0000000000000000 6 3 1150000000 p 15000000000000000 100000000 1 2 22 2006 158304 2006 6 22
35. 8 N 2 250000 X 8 200000 lt 150000 100000 5000 15 3 45 2 25 7 250000 8 300000 amp 250000 200000 150000 100000 50000 0 99 10000 2006158304000001 26 2006 158304 2006 6 22 72 72 72 72 48024 O 12 EAM 4B064 56 9 024 16 20 D0
36. 5 6 30 5 6 10 10 20 240 50 40 30 100 700 300 50 40000000 5 6 100 50 400 60 5 6 15 2006 158304 2006 6 22
37. 000000000 000000 00000000 10000000000000 10 000000000000000 000000 2 2006 158304 2006 6 22 900 1 OD oo 5x 10 mol Aspergi us 00000 Rhi 1000000000000 0 0
38. Tri coder m 100000 1 1000000000000 Aspergillus oryzae Escherichia col 1000000 9001110010 800 0 1000000000000000000 10 900 91000111010 11 1000000000000000000000000000 00000
39. i og Z 1 N F
40. 0 120 90 i dT30 lt Super gt 0 O 0000000000000 lug 1000000000000 0 0 0 Library Construction 00000000 0 0 0 10 Nap O O 50190 0000000 SMART 11 CSD 111 3 00000 141000000 72 210000 0 2u IO 5 5 Strand 1 20m M ODODO 1 10 dNTP 10 0 0 Rrasep 0 0 000 0O 42 cC0 1100 coap 0000000 29 10 00000000 100000001 10 Advantage 2 PCR 2u 50x dNTP 2 O 5 PCR pri mer 5 tcatcaccgg 33 2u 1 O CSD 111 3 PCR 2u 50 Advantage 2 0 0 220 95 2000 95 50 00 68 6 1000000 3000000000000 1 8 00000000 0 00000140 0 0 00 0 0 0 000 0 0 0 2360 0 0000 O O 2003 2651770 cerevisiae 0 0 0 0 0 0 6800 000000200 2001 2243811 000000 Aspergillus 0 0 sodM
41. 0 10u 10000 20m 00000 5 0 100 1 5 8904 100000 455 00000000 100000 20 1000000 0000000 2801 0000000000 00000 10 20 30 50
42. 700 600 500 R 400 300 lt 506 100 0 0 10 20 30 40 RISE mM 5 __ 2 mM E 7 0 19 20 40 EGED 8 R RK o H 5 iow N 2 7 1 g 5 R g 5 85 3 2006 158304 2006 6 22 5 6 20 7 280 280 280 350000 300000 250000 200000 gt 150000 100000 50000 0 4 4 0 15 30 45 59 50 350000 300000 250000 200000 150000 100000 lt 3 50000 15 30 45 FEARI 9 50 350000 300000 250000 200000 150000 lt 100000 50000 4 0 15 30 45 2 i 5 6 08 2 _ 280 280 280 2 280 50 350000 300000 250000 200000 150000 100000 50000 p 0 15 30 45
43. ooon O o N ON N i 1 1 21 2006 158304 2006 000000000000000000000 00000000 O pH6 0 1 5 6 5 6 13
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