Molecular Analyst /Macintosh
Contents
1. File Edit Object Analysis Filter Profile Regression MU Windows S M M AR OH profile 3 592 692 792 89 2 mm untitled 3 7 Profile Area Name i From To Width Height Location Area 96 of Total j Detection i 7 items mm I mm I mm 1 0D i mm i 0D mm 9 profile 2 area 2 3 1079e 0 Manual profile 2 area 3 2 6077e 0 Manual profile 2 area 4 3 4633e 0 Manual profile 2 area 5 1 7372e 0 Manual a profile 2 area 6 2 0294e 0 Manual profile 2 area 7 2 9693e 2 28 Profle PeakCritera Profle Find Peaks File Edit Object Analysis Filter Bite Regression MU Windows untit Average Profiles z Smooth Options Smooth Profiles 3M cos tes Use As Baseline Show Baseline Subtract Baseline Subtract Profile Point To Point Peak Criteria Find Peaks Hide Peaks List Peaks 292. Edit Select Al File Edit Object Analysis Filter Profile Regression MIB Windows untitled 2 y CIM profile 2 CIM profile 3 Name Standard Lane Color P File untitled 2 Selected Yes Image Example Image Shown Yes Origin 20 8 mm Type Profile Front 90 9 mm Points 702 Min Max increment Created Mon Jul 18 1994 12 49 09 PM Modified Mon Jul 18 1994 1 18 39 PM Comments 25 Profile 5 Smooth Profles File Edit Object Analysis Filter Il regression MIU Window Average Profiles m smooth Options untiti Smooth Profiles 30M Use Rs Baseline Hide Baseline Subtract Baseline Subtract Profile
3. Option Selection Tool Edit Object Analysis Filter Profile Regression MU Windows Example Image oe Nad RE ET CLA TD HEC Sra iE ts 1 e Kem ua A maya Soon rw A eae A Edit Select All Analysis Auto Integrate 7 i Integrate Now Results Window Object Analysis Filter Profile peg SEEL MU Windows Find Spots 36 F Spot Parameters Get Overlay Info XI File Edit
4. 116 0014 5 7 18 305 0031 1 15 1 222 0033 2 7 3 T465 0093 3 121 1 T 532 0025 1 14 11 812 0013 2 18 30 http discover bio rad cojp TEL 03 5811 6270 TEL 029 852 0835 TEL 045 476 0351 TEL 052 702 2358 TEL 06 6308 6568 TEL 092 475 4856 TEL 03 5811 6271 FAX 03 5811 6272 FAX 029 852 0829 FAX 045 467 0350 FAX 052 702 2812 FAX 06 6308 3064 FAX 092 475 4858 FAX 03 5811 6272 M3263 0506A
5. Adj Volume File Edit GP NESE Filter Profile Find Spots sr EB Spot Parameters Get Overlay Info 3l Object Auto Integrate S integrate Now ME ey Local Background EE T cue DEUM transformation XT EEE Extract Profiles st File Edit Object Analysis Filter Profile Regression MU Pr CC TT Example Image Be S G lt j Fr Joe n a eee Type Shape Rectangle X Center 298 35 mm Mean 2026 59 PD Y Center 275 70 mm Area 19 53 mm mm Width 6 70 mm Minimum 84 00 PD Length 3 40 mm Maximum 4227 00 PD Aspect Ratio 0 51 Porosity 0 14 Volume Volume 39579 27 PD mm mm Adj Volume 25075 06 PD mm mm Volume 36 16 Calibration Concentration Molecular Weight pl Comments 16 ype Standard
6. RAD VDL PUI TALLER FI TUR 38 Object Analysis Filter Profile untitled 2 r File Edit TETT p O4 Tus OM profile 3 SEP n cl o P E P M as e NN T Rud AN NE PS LEE m a b aA SEE STE aru ut e e PPI b el EC 3 3 3 eS D clos ont XC tata eS aal i d EE X 3 CS fetta ur we ud 0 PP a e DU e Pa Wawra Wey H EE er P alc s 4 00000 POX LESS GL one av Cr TROR e AAAS y EK A 10774 3638 1760 kilobases untitled 9 To Width I Height I Location 00 mm 0 0 mm 0 78 2990 3 5436e 0 0 78 3580 3 0656e 0 0 77 40 90 2 4659e 0 077 47 40 3 4317e 0 071 6040 1 7226e 0 61 80 68 30 650 0 74 6330 2 1291e 0 ze uan Unknown Lanzar 4 90 9020 200 es en Profile Area Name i From Area i 1 i 96 18 09 15 65 12 59 17 52 mm 32 80 38 50 43 80 53 80 61 70 i mm 10 00 5 60 5 20 9 90 5 90 7 items mm 22 80 32 90 38 60 43 90 55 80 Unknown Lane ar J Unknown Lane ar Unknown Lane ar Unknown Lane ar Unknown L ane ar g Unknown Lane ar rd Results Window Excel
7. oo of Total Detection Automatic Autornatic Automatic Automatic Automatic Automatic Regression MIU Windows Calculated MW kilobases 9 31244 10071 6521 4406 2388 Autamatic 5 A M DeL eee LES em Ls Preferences Rile Preferences Print image on single page ae pc e o Preferences ERE Saving pee 3 Print image on single page Printing A mS Imager ok Print image on single page Saving Filter Printing Print image single page Image Filter 39
8. Profle HList Peaks 30 File Edit Object Analysis Filter Bint Regression MU Windows untiti Average Profiles gt gt gt Smooth Options Smooth Profiles 36M Use fis Baseline Show Baseline Subtract Baseline Subtract Profile Point To Point 2 DM profile 3 0 90 0 75 0 60 0 D Peak Criteria Find Peaks 36 F Hide Peaks List Peaks SS OGA KR 0 00 AS SA Scaling s KY sf 192 292 39 od Get Profile Info 29361 untitled 4 Width Height Location Area of Total j Detection mm 0D I mm i 0D mm W 0 45 0 30 Profile Area Name From To 1 7 items mm i mm profile 2 area 2 profile 2 area 3 profile 2 area 4 profile 2 area 5 profile 2 area 6 profile 2 area 7 3 0656e 0 2 4659e 0 3 431 7e 0 1 7226e 0 2 1291e 0 2 6750e 0 Automatic Automatic Automatic Automatic Automatic Automatic File Edit Object finalysis Filter Profile Regression MI Windows profile 2 area 9 Info Name profile 2 area 9 Profile profile 2 File untitled 2 RES OSV Ar Location
9. DP CE X Je Pr Pe oP K P gt um g ERU A 3 gt he eS KES Grid Preferences Mi Windows ession Object File Edi Filter Profile Regt Analysis Q I heg I EI E oi EI EE ae A ET lede rR Me Me ak Ree ie We Or t E ee aTa ee ee del ae X d ae tme Nadie roer Core rre had had TETTE TIC Cer meterle Kant ea Eg EM Se Sn Tie ra VEM TA I EU TY SC Ec e SR gi erar a ctor en aen ed Sean CAN Se DAL DE ME WC den SOR E eaa Cm eam i RR 12 6 File Edi f Windows MLI dd DM deal n rh an a ay Ate 1 Profile Regression Analysis Filter Example Image Object er ae ih Pe i rad EA BAUR Pi de 1 FE OA dde tata 13 14
10. File Edit Object Analysis Filter Profile Regression MU Windows n Preview r Data Range Minimum 0 Maximum 4227 Transformation Values Minimum lo Maximum 10 Color Palette File Edit Object Co 4d A La Analysis Filter Profile Regresston MIU E22 eee Color Palette Paint E Red v Palette qs fus ter Weighted Noise Reduction Filter Size 5 X 5 Boost Factor 1 OK Smoothing Noise Reduction Weighted Noise Reduction m nM Sharpen Sharpen In Place Convolve BW MTM ORPA ANR Edit Undo 11 Volume Analysis A Object KI SS EN kam
11. Calibration Concentration Molecular Weight pl Edit Object Analysis Filter Profile Regression MU Windows Example Imag Name Standard 1 Type Standard w Shape Rectangle X Center 298 35 mm Mean 2026 59 PD Y Center 275 70 mm Area 19 53 mm mm Width 6 70 mm Minimum 84 00 PD Length 2 40 mm Maximum 4227 00 CPD Aspect Ratio 0 51 Porosity 0 14 Volume Volume 39579 27 PD mm mm Adj Volume 25075 06 PD mm mm Volume 36 16 Calibration Concentration Molecular Weight pl Comments 17 Object Size a Ge a File Edit lad Analysis Filter Profile Regression MID Windows 10 Point 12 Point 14 Point 18 Point Grid Parameters 24Point it wats MERE 36 Point 48 Point 42 Point A Other x E E o uc nd ge ad 100 ag lt E Get Ru i Hs HRS Rep 1 SUSUR Nhe Eph JH uit inus fb Window Regression v Regression Curve Analysis Filter Profile Regression MI BIIUTDIBDITIES EE i Example Image Palette NSR SD Prepie
12. Point To Point Peak Criteria Find Peaks HF Hide Peaks List Peaks Profile Use as Baseline File Edit Object Analysis Filter AXXUIIJM Regression MID Window Smooth Options Smooth Profiles 3M Use As Baseline Hide Baseline Subtract Baseline Subtract Profile Point To Point KI profile HIME profile 2 Peak Criteria Find Peaks Hide Peaks List Peaks Get Profile Info 2l 26 lt Shift gt Profile Subtract Baseline File Edit Object Analysis Filter MIELE Regression MU Example Image Average Profiles MR E nntitl Smooth Options Smooth Profiles 36M Unuse As Baseline Hide Baseline Subtract Baseline Subtract Profile Point To Point Window ip MOE i PETI XM profile 3 Peak Criteria Find Peaks 3sF Hide Peaks List Peaks 21
13. 5 6 7 amp 9 1011 1213 14 15 16 17 18 19 20 2122 23 24 23 Rcquire i cm C Show Grid FRirssysdsassurAag NknsaPuarsantevquedereuuenasrecenane run FraasYsdat t capsaus ZitETNAnausuaETcsuI GR4CA4A PI SR RH un eta aua B D E F G H I 7 K L M N o P Q R T kent 4 94 EC MA File Size P bengh 0 04 Me h 3 Status Idle es M Preview 100zm 200 um Resolution Acquire Untitled hmnagee M M A M 8 Huc us tu cu Ut B 525 Molecular Imager FSR y Units Avi V eg PE aksent ME 2 5 4567 9 1011121314 15 16 17 18 19 20 2122 23 24 23 C quadrant O cm Show Grid att a Resolution 10 Good 800 pm C Better 200 pm Cm Kop erre Left Top E Right 14 50 Bottom M width e o m y mo m b d Doc m File Size P Length 0 02 MB eer eer eee errr rec Trees ae gli ae aa Status Idle o Ne 2 1 B c D E E H 1 K L M N o P Q R T HE VRD AV NERO v 7vMoSSXSd4UCOEX S
14. Format TIEF PC Format File Export E a i Example image MB i3 trample image PR Export Image As File Type TIFF PC Format 2 Raw Data owe NE vm PA EP a neun pn NE CODO D o o Gr RO P Cn a t BB AO DNI ARE Manipulating Images eut v 7 AT ao w Edit Extract Crop PR a UR yw pels od at ye Tad E 6 OUT rks wads eae Pa TE ee i n E ER dS UE SNP EO eh ud 7 p p rea he wey LT 3 ed ne m Example Image Horizantal ip Vertical Selected ip Duplicate Select All Bestore Fi FI 3 L E p c A A eo how en QJ c e 3 c PM a sax bin p E e c aa sje e e q Li od ad cr 1 z e as y TRIAL tSave As Save Ores dog File Analysis Transformation Preview Preview Se DS Che CE WS
15. Rf MW Calculated MW I Residual Error S 7 items i mm i kilobases i kilobases kilobases 3 Er 1 Fragment 1 23130 00 24776 93 1646 93 2368 22 Fragment 2 Fragment 3 B Fragment 4 Fragment 5 Marker 6 9416 00 6557 00 361 00 2322 00 2027 00 546 00 9645 78 6321 42 4245 12 2373 25 2104 56 458 00 229 78 233 98 115 88 51 25 77 06 88 00 2368 22 2368 22 2368 22 2368 22 2368 22 2368 22 File Prmt 36 VA Sea gy ue beoe Se BAO Jose se 5 e File Edit Object Analysis Filter Profile Regression MW Windows x 194 kilob 10774 3638 1760 lt gt X E x MM File Edit Object Analysis Filter Profile Regression MU Windows untitled 2 DE DIT at ane thro Veg 315 BIO G I SO Sa x Y FE kd TN CIM profile 3 a X 2317kilob 10774 3638 1760 Y 0070D v 06000 cilebeses 37 Profile 5 Find Peaks Profle List Peaks Results Window
16. f 77v50rvy7T ate lL MIT TC RF xry TAZ ESTC 35 X 43cm Preview Units mee 8 4 ere 2 4 6 8 10 12 14 16 18 20 22 24 26 28 32 34 Acquire Require JM Q em gt Show Grid p LI x e Resolution Cod 800 Wm i Better 200 pm info i Best 100 um i I Section esos toe li Right 26 11 Bottom x e xm UO dg M File Size Lengthy 0 14 MB lt lt 4H cc Oo Zz Status idle Z Lolo cc f 168 525 Molecular Imager 7 RE amp Preview O dean 2 4 6 9 10 12 14 16 18 20 22 24 28 28 30 32 34 quadran Require Require Q om E Show Grid i Resolution j Good 800 um Better 200 pm O Best 100 um rSection Left Z Top Right 922 22 Bottom vw Wig File Size Leagh 0 04 MB Untitled Image FHile Save MG o object Analysis Filter Profile Regression MW Windows Save As Revert to Saved Acquire Import Export Get Image Info Preferences Page Setup Print Save Bio Rad
17. reset a me CC m e AAN det EN Lid e sae oa v atra Pi was A PE eee ret Et e debt qr a V dele Hb m wot em deb vidc gi dei Pe ie ar a ap ee ale fj e em a p r fa P E or Yam dea E d le Mehta Remit i eke bs ien C rede nite VES ve or Rel Siete ee a SAP LPS T Apr rS ona eere ide rendi Soc eai Mes Lr T did race rev mcn TOS ad vi ull cH FP M OO AS Pb GEAR SA E aet eR 5 TI Ger EEE HUME T LETTET ee eee cee oes oa untitled 3 Volume Name Type Mean Area Min Max Volume Adj Volume 96 Volume Concentration ens 1 PD mm mm PD PD PD mm mm PD mm mm Unknown 586 03 2295 00 1 1445e 4 9 4873e 3 Unknown 1133 24 3212 00 2 2132e 4 1 8066e 4 Unknown 1289 08 4058 00 2 5176e 4 1 6724e 4 Unknown 2026 59 4227 00 3 957 9e 4 2 5075e 4 LER E D UO 15 Analysis Local Backeround Auto Imtegrate Background Auto Imtegrate Analysis 5 Auto Integrate Integrate Now
18. 2 mm BANTIITyYTENRNIYTLT FUOITANORALRACREUTSC ESL File Edit Object Analysis Filter Profile Regression MID Windows e untitled 2 E CW profile 2 OG profile 3 592 639 2 mm 32 OK File Edit Object Analysis Filter Profile Regression MID Windows untitled 2 AE DIS EP Ga 1 20 AI IW Standard L 1 05 OW profile 2 OM profile 3 0 90 0 75 2 060 0 45 0 30 0 15 0 00 x 192 292 392 Y Y EM ae AG mm Name MW Units d m i MW Marker 1 Info MY 100000 00 Units kilobases Ruler untitled 6 Created Mon Jul 18 1994 1 41 47 PM Modified Mon Jul 18 1994 1 41 51 PM Comments 33 lt gt X amp amp mm File Edit Object Analysis Filter Profile Regression MID Windows m untitled 2 Hc CIN profile 2 OB profile 3 24296 15327 File Edit Object Analysis Filter Profile Regression MU BUTT
19. 35 8 mm Height 0 780D Area 5 07 O0 D mm of Total 15 65 95 TRO XR EXC BOOS RS M eee KKN a ai S 24 re E 32 9 mm To 38 5 mm From Detection Automatic X Created Mon Jul 18 1994 1 26 PM Modified Mon Jul 18 1994 1 26 PM H R Comments Profile r Area of Total Detection 14 items DD mm 0656e 0 Automatic profile 2 area 10 38 60 4380 5 20 0 77 40 90 2 4659e 0 12 59 Automatic profile 2 area11 43 90 53 80 9 90 0 77 47 40 3 431 7e 0 17 52 Automatic profile 2 area 12 35 80 61 70 5 90 071 60 40 1 226e 0 8 79 Automatic profile 2 area 13 61 80 68 30 650 0 74 63 30 21291e 0 10 87 Automatic 2 6750e 0 Automatic profile 2 area 14 File Save Prmnt EH MTT Results File Export Print 31 Molecular Weight Determination MW New Ruler File Edit Object S8nalysis Filter Profile Regression MUI Windows SSS untitled 2 New Ruler Open Set Save Set As profile 2 Show Ruler OOM profile 3 List 492 59
20. Molecular Analyst Macintosh 4S 1999 Molecular Analyst Macintosh PDF PDF H4 6 db 5 2 E HO x23 CARS TOS VN 2005 F 6 H Multi Resolution Scanning Manipulating Images Volume Analysis Profile Analysis Molecular Weight Determination Preferences 12 21 32 39 Multi Resolution Scanning Molecular Analyst Rppiet B Audio Player Automated Tasks Calculator c Chooser Control Panels 2 Find File amp Graphing Calculator Al Jigsa
21. b MILLE detect et ET rman poe Teo kn La T ie ibm lt Option gt Sh lt Delete gt File Edit Object Analysis Filter Profile Eaz Example Image 5 23 Edit SelectAll Analysis Extract Profiles Profle Window File Edit Object BNR Filter Profile Spot Parameters Get Overlay Info 1 Auto Integrate Integrate Now Local Background Transformation amp T Extract Profiles To Wo x 24 untitled A XM profile DIM profile 2 PP Boratile 3
22. i OD mm rom OD mm mm Save Save As Revert to Saved 4 1844e 4 3 5315e 4 2 5673e 4 2 4272e 4 1 6822e 4 1 0732e 4 3 8163e 3 2 3156e 3 1 0000e 2 6 0000e 1 8642e 1 1 0000e 1 ficquire import Get Image Info Preferences Page Setup Lm Print JP M Quit 38Q a PExcel Sh Excel Edit Paste File ui Formula uus Data Options mairo windon aia 098 WE _Stndad 058 Ala 05 08 243804 132803 887 100801 MN NONE nine NUN MEN ae eu k 20 Profile Analysis dw 160 JD Pont to Point 3 File Edit Object Analysis Filter Profile Regression MW Windows b pt ai ae iyi 21 22 File Edit Object Anaiysis Filter Profile Example image R
23. untitled 2 Palette P D Preview 1 20 Regression 4 p Dom HEZPEINN 1 05 untitled 2 OW profile 2 Example Image CIIM profile 3 0 90 0 75 0 60 0 45 10777 3638 1760 kilobases 34 Object Analysis Filter Profile BLUES MW Windows untitled Rei MIU vs Rf Lag MIU vs Rf Molecular Weight Regression fd 10 Linear Quadratic Cubi Fragm nt 7 PT m 08 Logistic c Volume c Ab lume d 06 s lJa e o 2 gt 0 4 a 0 2 o Fragment 1 0 0 5 00e 03 2 00e 03 3 00e 03 1 60e 04 2 30e 04 3 DOe 04 Molecular Weight Logistic Y 8 84e 0 1 8 32e 02 1 x 2 47e 03 1 32e 00 9 32e 02 Regression Linear Quadratic Cubic Logistc Results Window 35 MW List File Edit Object Analysis Filter Profile Regression III Windows 2 yntitied 2 New Ruler 3R Ke l ES Go Open Set 1 20 A AA Save Set As Stand e 1 05 Hide Ruler List x gt BE Reset H Axis Ts et OW profile 2 CIM profile 3 0 90 0 05 o 0 60 0 45 i 0 30 HE 0 15 5 0 00 Br 10777 3638 1760 pus o 2n untitled jj Standards Name Index j Mobility
24. w Regression Example Image untitled 18 Regression Linear Quadratic Cubic Logistic Analysis Auto Integrate Results Window Object Analysis Filter Example Ima Profile ROLURI MU Windows eRMU sar peas Log MW vs Rf Linear Quadratic Cubic Logistic hopet Ss ane Concentration Regression for Overla 5 00e 04 4 10e 04 Volume Abs Volume Show Names Show Errors 3 20e 04 2 30e 04 1 40e 04 5 00e 03 0 00e 00 2 20e 01 4 40e 01 6 60e 01 8 80e401 1 10e 02 Concentration Quadratic y 8 78e 03 2 68e 02 x 4 95e O1 xA2 r 2 1 0000 Data Type a Concentration O MY Q p Eile Pnnt REIile Export Excel Pnnt FT Edit Object Analysis Filter Profile Regression MW Windows Sl untitled 3 Area Min Max Volume Adj Volume Volume Concentration Cram mm OD
25. w Puzzle laj Key Caps Note Pad Recent Applications 5 Recent Documents Scrapbook W Stickies Ej Shut Down Eile Acquire Molecular Imager BW Close Saue Save As Object Revert to Saved Acquire Import Export Get Image Inf Preferences Page Setup Print gt 0 Analysis imagina Densitometer Molecular Imager xp MM PO Profile ies EST ML Windows Color Palette s Hit Hu HAT ROPTE TT FRATA ASONO P i trii ay delays oa L XP POEM Stater PEE HM PORE UHR Ly Palette Grey Scale Y esti Ge A Preview 800 m Resolution M RD Color Palette h FORE TER ore as ee ee Se SM D TIER EA rut zd inge ee em core i GS 525 Molecular Imager nud pun gt Units Tm t E mm T Sn 3 4
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