DS-VS取説 - 株式会社リーゾ
Contents
1. 1 6 DNA 6 1 1 v v 7O TE 10 mM Tris HCL 1 mM EDTA pH 8O pH 8 O 1 1 25 24 1 SIGMA Cat No P20692. 7 3 1 5 m 1 000 200 gl 5 ml DNA DNA 1 VS 1 4C DNA DNA GO ml 4C DNA 1 720 ul DNA VS 8O1 10 11 12 13 1 5 m 400 1
3. DNA VS 30 50 mg 3 400 1 DNA VS 1 1 v Y 500 1 15 OOO rom lt 20 25 C 10 6OO gl 15ml 99 59 48O gl O 8 3 OOO rpm 1 7OO 1 5 ml DNA 7OO gl 15 OOO rpm 10 7O 1 000 gl 15 OOO rpm 4 10 50 100 1
4. 5 7O DNA 18S rRNA 1 146 bp PCR M 1 2 3 4 5 6 Ui 8S 9 1 5 Agarose Mi Marker 100 base pair ladder 1 6 2 7 3 8 4 9 5 50 mg DNA PCR DD Template DNA 1 1 3 10xBuffer 2 dNTP mixture 2 0mM each 2 primer 4 pmol each 1 2 Tag 5units 1 0O 17 HsO Total 20 DNA 2 100 DNA Stratagene Paq5OOO PCR 95C 2 min 94C 30 sec 55C 30 sec 35 cycles 72C 60 sec 72C 7 min
5. DNA TE PCR DNA DNA DNA DNA DNA 4
6. DNA UF 2 9 2 B201 029 852 9351 FAX 020 4623 5611 E mail info rizo co jp http www rizo co jp Copyright e2009 201 1 RIZO Inc All Right Reserved
7. DNA DS OOO4 DNA Ver 1 0 RIZO Inc DNA 0 NN 0 0O 0 O I 7 DNA DNA PCR DNA 85mL 110 2 10 mLX1 DNA 6O ml 4C
8. TE PCR 5 ea DNA V MA VS 1 EAA DNA PCR 1 0001 G6 5 7
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