PDFファイル - 医薬品医療機器総合機構
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1. 7 8 2 8C 12 IO PE CD19 IOB4a A07769 100 2mL 1 Doody G M Dempsey P W Fearon D T Activation of B Ilymphocytes integrating signals from CD19 CD22 and FcyRIlb1 1996 Cur Opin Immunol 8 378 382 2 Bradbuy2. 5 Ficoll Paque 8 9
3. 1 2 11 1 20L 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 BSA 0 5 0 1 2 PBS VersaLyse PBS 1mL IOTest 3 A07800 12 5L 0 1 PBS VersaLyse PBS 1mL IOTest 3 A07800 67 5 L 0 5
4. o 95 20 40 CD14 Raji 1 0 5 1 Raji Burkitt lyphoma PBS 4 3 10 3 5 5 1 0 196
5. PBS 2 8 C 400xg 3 10 PBS 5x103 mm3 5x106 mL 11 90 1 12mmex75mm 2 Ficoll Paque 5x10 5x10 mL 100uL 3 20uL IOTest Mouse lgG1 PE 20uL 4 2 8 30 5 PBS 2mL 2 8 C 400xg 5 6 5 7 0 5 1mL 0 5 PBS
6. PBS 3 A09777 VersaLyse IM1400 OptiLyse B A11895 OptiLyse C A07799 IOTest 3 4 IOTest Mouse IgG1 PE A07796 100 2mL 5 Ficol Paque Phamacia 17 0840 03 2 1 100uL 5x103 mm3 10x103 mm3 PBS 3x103 mm3 1 25C 500xg 5
7. 2 3 4 DxH800 5 PBS 5 10x103 mm3 1 12mmex75mm 2 100uL 3 20uL IOTest Mouse lgG1 PE 20uL 4 15 5 6 PBS 2mL
8. 7 400xg 3 8 6 7 9 0 5 1mL 0 5 PBS 10 24 3 Ficoll Paque 1 3 4mL PBS 2 Ficoll Paque 4mL 1 3 2 8 C 400xg 30 4 Ficol Paque PBS 28 C 400xg 8 PBS 2 8 C 400xg 4
9. 8 24 FS 90 SS PE Log 29
10. 29 EPICS XL vs PE Lumphocutes Leukocutes w Ee a S lt ee 1 1 19 199 1 99 1 1 19 199 1888 RD1 RD1 CYTO TROL 6604248 Fc IOTest CD45 FITC CD14 PE A07738 12100 02282 3 0 8
11. T NK CD19 B J4 119 J3 119 IgG1 SKLY18 PE Phycoerythrin 486 575nm 560 590nm CD19 CD19 PE 1 IO 2 EDTA 3 6 4
12. 2012 10 1 20200EZY00111000 A07769 Wa COULTER I B IO PE CD19 IOB4a 1 2 3 4 IO PE CD19 IOB4a PE IgG CD19 95kD CD19 B pan B
13. L E Kansas G S Levy S Evans R L Tedder T F The CD19 CD21 signal transducing complex of human B Iymphocytes includes the target of antiproliferative antibody 1 and Leu 13 molecules 1992 J Immunol 9 149 2841 2850 3 Carter R H Doody G M Bolen J B Fearon D T Membrane lgM induced tyrosine phosphorylation of CD19 requires a CD19 domain that mediates association with components of the B cell antigen receptor complex 1997 J Immunol 158 3062 3069 4 Tedder T F Zhou L J Engel P The CD19 CD21 signal transduction complex of B lymphocytes 1994 Immunol Today 9 15 437 442 5 Tedder F Isaacs C M Penta A Cloning and structure of CD19 a member of the immunoglobulin superfamily Use of transfected cells to examine the workshop antibodies 1989 Leucocyte Typing IV White Cell Differentiation Antigens W Knapp et al Eds Oxford University Press 36 38 Foon KA and Todd RF 1986 Blood 68 1 31 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460 135 0063 5 7 TOC wm oo A07769_1210
14. T B NK 1O
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