J5-FITC
Contents
1. 5 6 Ekik CD10 7 FITC RD1 PE FITC RD1 PE2. a gt 10 x 10 mm 10 20 x 10 2 20 30 x 10 3 30 40 x10 5 40 60 x 10 6 60 100 x 10 10 100 200 x10 20 b 3 x 10 mm 1 25 500 xg 2 IOR FRATO 3 4 LH700 5 3 10 x10 Emm Q Prep
3. 3 4 3 4 5 6 7 8
4. CD10 5 11 10 000 uL E Ri CDO 3 CD10 CD10 149 CD10 CCD10 3 CD10 CD10 1496 ERM CDI 3 CD10 596 4 IE RAIRE CD10 3 CD10 59 CD10 CCD10
5. CD10 B B J5 CALLA T ALL BALB cJ NS 1 AG4 Ig lgG2a FITC Fluorescein Isothyocyanate FITC 3 gt 10 468 509nm 504 541nm 1 4 CD10 488nm 525nm
6. 1 2 859 3 EDTA 6 24 2 4 909 5 10 000 mm 10
7. CYTO STATCOULTER CLONE MSIgG2a FITC 1 29 29 non T
8. 12mm x 75mm 2 100L 3 10uxL CYTO STATCOULTER CLONE MSlgG2a FITC 10L 4 10 TQ Prep Q Prep 1 0 5mL 2 11 2 2 2 8 C
9. 11 PBS 3mL 400 450 xg 5 PBS CO 10 uz 1 7546999 300 TQ Prep Multi Q Prep 7546946 100 Q Prep FB a A b B c C 6603152
10. 2009 11 6604107 2007 2 21800AMY10098000 CALLA J5 FITC 1 2 3 4 1 CD10 J5 0 69 u gr AF FITC 1 0 5mL BSA 0 296 0 01M NaCl 0 15M NaN 0 1 CD10 100kDa CD10
11. non T ALL CD10 CD10 Common Acute Lymphoblastic Leukemia Antigen CALLA 100kDa II ZH RS E EA C CD10 B CD10 B CALLA non T T J5 CD10 CD10 CD10 CD10
12. J5 FITC 2 8 C 18 J5 FITC 6604107 50 0 5mL 1 Ritz J Pesando JM Notis McConarty J Lazarus H Schlossman SF 1980 A monoclonal antibody to human acute lymphoblastic leukemia antigen Nature 283 583 2 Ritz J Nadler LM Bhan AJ Notis McConarty J Pesando JM Schlossman SF 1981 Expression of common acute lymphoblastic leukemia antigen CALLA by lymphomas of B cell and T cell lineage Blood 48 648 3 Clavell LA Lipton JM Blast RC Kudish M Pesando JM Schlossman SF Ritz J 1981 Absence of common ALL antigen on normal bipotent myeloid erythroid and granulocytic progenitors Blood 58 333 4 Sallan SE Ritz J Pesando JM Gelber R O Brien C Hitchcock S Coral FS Schlossman SF 1980 Cell surface antigens Prognostic implication in childhood acute lymphoblastic leukemia Blood 55 395 5 Greaves MF 1981 Monoclonal antibodies as probes for leukemic heterogeneity and hemopoetic differentiation In Leukemia Markers ed W Knapp Academic Press p 19 6 Greaves MF 1981 Biological basis of differential prognosis in immunologically defined sub classes of acute lymphoblastic leukemia In Leukemia markers ed W Knapp Academic Press p 463 7 Nadler LM Ritz J Todd RFIII Griffin J Reinherz EL Schlossman SF 1981 Diagnosis and treatment of human leuk
13. 3 CD10 596 CD10 3 CD10 CV 5 2 100 CD10 y 1 004x 6 416 r 0 984 n 50 c u LO 73 2 ALL ALL 1 2
14. CD10 n 20 CD10 2 0 0 396 1 2 Ekik CD10 lt 3 FITC RD1 1 RD1 J5 RD1 J5 FITC 4
15. 24 C1 O 2 FS 90 SS 3 FS 3 FITC Log 1 2 4 EPICS XL FS SS FITC FITC Fi ngabed ZO176EOLMD SUFE FTHLmmhl Z0 7630 LMD FL1 LOG F1 IMHC ZD01 7680 LID FL1 LOG ui Pu J mne f a Lymph E E 1023 1 Tpi TE WW amp 8 8 JB FTTE FITE L 87 154 i den CE J5 FITC
16. 2 PBS PBS 6603369 1 500mL pH 7 22 0 2 3 CYTO STAT COULTER CLONE MslgG2a FITC 6603855 50 0 5mL EDTA 1 100 L 1 1 2mL 3 10x10 48 mm 20 25 6
17. 000 mm 6 7 9 CYTO STAT COULTER CLONE MSIgG2a FITC 10
18. 15 Taki T Ida K Bessho F Hanada H Kikuchi A Yamamoto K Sako M Tsuchida M Seto M Ueda R Hayashi Y 1996 Frequency and clinical significance of the MLL gene rearrangemants in infant acute leukemia Leukemia 10 1303 1307 16 Heerema NA Maben KD Bernstein J Breitfeld PP Neiman RS Vance GH 1996 Dicentric 9 20 p11 q11 identified by fluorescence in situ hybridization in four pediatric acute lymphoblastic leukemia patients Cancer Genet Cytogenet 92 1 11 115 17 Ganju RK Shpektor RG Brenner DG Shipp MA 1996 CD10 neutral endopeptidase 24 11 is phosphorylated by casein kinase Il and coassociates other phosphoproteins including the lyn src related kinase Blood 88 4159 4165 135 0063 5 7 TOC TEL 0120 566 730 _ FAX 03 5530 2460 Bl Ro E 135 0063 5 7 TOC oe COULTER 4 4 6604107 0911
19. emia and lymphomas utilizing monoclonal antibodies Progress in Hematology ed E Brown Grune and Stratton Inc NY p 187 8 Cossman J Neckers LM Leonard WJ Greene WC 1983 Polymorphonuclear neutrophils express the common acute lymphoblastic leukemia antigen J Exp Med 157 1064 9 Hokland P Ritz J Schlossman SF Nadler LM 1985 Orderly expression of B cell antigens during the in vitro differentiation of nonmalignant human pre B cells J Immunol 135 1746 10 Boucheix C Perrot JY Mirshahi M Giannoni M Billard F Bernadou A Rosenfeld C 1985 A new set of monoclonal antibodies against acute lymphoblastic leukemia Leuk res 9 5 597 604 11 Boucheix C Perrot JY Mirshahi M Bernadou A Rosenfeld C 1983 A rapid method for detection of membrane amtigens by Fluorescent microscopy and its application to screening hybridoma antibodies J Immunol Methods 57 145 150 12 Lebien WT Mac Cormach RT 1989 The common acute lymphoblastic leukemia antigen CD10 Emancipation from a functional Enigma Blood 73 3 625 635 13 Penchansky L Pirrotta V Kaplan SS 1993 Flow cytometric study of the expression of neutral endopeptidase CD10 CALLA on the surface of newborn granulocytes Mod Pathol 6 414 418 14 Connelly JC Chambless R Holiday D Chittenden K Johnson AR 1993 Up regulation of neutral endopeptidase CALLA in human neutrophils by granulocyte macrophage colony stimulating factor J Leukoc Biol 53 685 690
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