マニュアル AKTApurifier UNICORN 5.0
Contents
1. OFF TEL 03 5331 9336 EA 9 00 17 302. 60 S ON TEEI 9
3. 18 1132 75 13 3 3 2 1 2
4. 18 1154 17 1 2 3 61 12 1 2HD DOS V 1 44MB 1 A 2 Windows My Computer P y ompa 3 3 1 2 Floppy A
5. AKTA 0 35 MPa HiPrep 0 15 MPa FR 902 0 2 MPa HiTrap AKTA HiTrap 0 5 MPa HiTrap 0 3 MPa FR 902 0 2 MPa 4 4 1 Manual Pump PumpwashPurifier ON OFF ON OFF gt 1 A1 2
6. 1 2 Pause Continue 3 4
7. 2 3 3 0 00MPa 0 05MPa No Yes Check 3 Act 1 0 15 MPa 2 SystemControl System Calibrate P 900 Press Calibrate Act 1 Gradient ConcB O0 Flo
8. System Control System Settings Specials FracParameters kiem Miale baira rikar FI p mew F mpa Paa Tie F p a hi iie aan Riga D I muni baraa S D i Timige 1 TI mem W mfike i DER a a FT Accumulator pHCell pHCel 0 25 mm 238 ul A 0 5 mm Bu SAWM 0 25 mm 88 ul 0 5 mm 232 l 354 Hl PEEK pH Cell PEEK mm ul 10 cm HR PEEK 0 25 4 91 PEEK 0 5 19 6 AKTApurifier 29 6 2 Frac 900 6 2 1
9. 2 6 18 1112 51 Superloop 150 ml 50 ml 10 ml i L Superloop 10 ml 50 ml 4 MPa Superloop 150 ml 2 MPa 21 sssi amp Frac 950 Frac 900 6 1 Frac 950 6 1 1 DropSync
10. AKTA 2 5 4 4 e 9 7 sa 10 20 108 Evaluation 98 AKTA 108 EE 2 1 UNICORN AKTA 2 1 1 CU 950 PC 4
11. On hne Help 1 1 AKTApurifier INV 907 Box 900 2 pH C 900 PV 908 UV 900 s M 925 P 903 W1 W2 and W3 A B UV SV 903 SV 903 180 mm 130 mm 4 D G5 L o sus CONDp am Me G6 s G9 H9 HG x L Juv H8 A G B G GA A3 B3 D a NC NO NC NO A2 Al B2 B1 W2 Wi NS W3 gc6 J CU JK L 4 F3 AKTApurifier J P 903 2 0 001 10ml min 25MPa UV 900 190 700nm UV Vis
12. Inject 2 Waste PumpWash Column Column Column Sample syringe Waste Waste Load V1 2 6 10 nl 100 il 1 ml 150 ml 10 ml 50 ml 150 ml 3 FR 902 0 2 MPa FR 904 0 4 MPa 2 FR 902
13. Auto Fixed 3 Adjust retention zero to injection number 0 min ml 4 OK m 54 9 2 3 1 2 3 4 Page Down Page Up 5 6 Reset zoom 33 9 2 4 1
14. 3 Column Load 3 3 4 4 3 4 43 8 3 Frac 950 6 1 1 Frac 900 _ Outletvalve F3 F3
15. 1 Fie Print Print Chromatograms Arini Le TinPY ama LLP Pe Ei iB Er F Ep Kikash r pek j 2 Print Format 3 Landscape 4 Preview Customise Report Exit 5 OK m 56 I 2 20 10 1 RY TAF System Control 1 Manual Pump PumpWash Purifier ON OFF ON OFF
16. Rename a 3 0OK Check 1 AKTApurifier Pause Continue lt lt No Yes Act 2 Check 2 GRIE 1 Manual Pump 1 PumpWashPurifier A1 B1 gt Execute 2 Manual Pump Flow gt 5 ml min gt Execute 5 END 67 AKTApurifier A 2 1
17. Add Text 2 abc 3 abc 4 text gt text gt 5 amp 1 Edit Text Mode 2 3 9 3
18. 4 New Group 5 OK 8 7 2 Curves M7 I Li 8 s ltr j T F TE j A peN sd A F i i k I H Fa kI p k Propri nagF kk Ei laa Fop Mg 1 KE SD I E 1 Curves 2 Properties Curves Fira parli Curs This asaj Dulu Tin mh Fiam Lim ea Rnb Daka Lur 3 Select All 4 OK 1 Curves Marker 2 3
19. Flowthrough Fractionation Frac 950 OutletValveF3 OFF Frac 950 33 Fraction Volume Tube Type 18 mm 30 mm Start at Next tube First tube 1 Fraction Volume 50 ml Tube Type 30 mm Start at First Tube Next gt Elution Fractionation Frac 950 Frac 950 OFF OutletValve OutletValve Frac 950 Frac 950 3 Fixed Volume and Peak Fractionation Fixed Volume Fractionation Peak Fractionation liz Fixed Volume Fractionation Fraction Volume Tube Type 18 mm
20. 1 5 5 mm Lock knob 6 PEEK 5 mm T Tutung hude Ei 27 7 PEEK 8 1 LGI Ta Lock Knob 5 mm
21. 2 nm 1 ml mn 0 35 MPa 2M NaOH 18 1120 94 2 N N TA 11 4 11 0004 14 1 5 Frac 900 gt 19 6067 02 lt 11 6
22. FR 902 FR 904 2 2 nm 2M NaOH 18 1120 94 2 67 11 3 1 2 AKTApurifier amp O 500 ml ARAR T LIESEL 20
23. aa 8 4 1 System Control File Run Mam np IEE SFF Bm Tp Feie TIA merie Fe EEE meii HI meri Fa TED Meal Fan Li Mep Fi LT ehej Ai 2 OK 3 Next gt 4 Frac 950 Rack Fraction order LE 5 Start Next gt Stat i m 44 8 5 1 END
24. waste W1 15 3 6 1 UV G5 2 Manual Pump Flow 5mMmin gt m um s 5 UV Cond Pressure 3 END gt gt Load gt UMV gt W3 G5 gt Pi 4 1 AKTAdesiszn 1 16 HHiTrap FPLC HR M6 2004 10 HiTrap
25. 10 2 1 Pressure limit Manual Alarms amp Mon Alarm_Pressure High Alarm 2 Manual Pump Flow gt 3 3 UV Cond Pressure END 4 UV G5 5 Manual Flowpath Injection Valve Inject Manual Pump Flow 5ml min gt 6 5 END 10 3 lt Frac 950 gt 1 Manual Pump gt Flow 2mMmin gt 2 Manual Flowpath OutletValve gt F2 gt 3 Accumulator Accumulator Manual 1 Frac AccumulatorWash Strokes 5 4 Accumulator Manual Flowpath OutletValve gt F3 gt 5 3
26. 3 30 mm Eppendorf tube holder for Tube Rack 175X12 mm 0 1 00 6 2 2 pH System Control System Settings Specials FracParameters_900 kaien S bale iriri rikar IEm e q SUL aen hinallas i LSID mun ID IEEE A Fm arsusa HHI n Pa Pen N ukumi Fm C Tiba Ei Egip Egej F ae T Ghj U asi iha pH Cell pH Cell 0 25mm 88 ul 0 5mm 232 lI 354 yl
27. 2 mMmin Accumulator accumulator 2 mMmin Accumulator Tube DropSync Accumulator OFF System Control System Settings Specials FracParameters ms a mam gi keim M brair LE kari Ira p ipiri Fri eee arma Cam Fm x Disa oiii vai r w e C LEO ad mam m sa Fii _ h r Eai L E ma T iacF paria l Ve miiie T ruan re TD muni re CE ml mt TL el Le II em Fm or i 6 12 V4 Frac 950 E 2 ml min DropSync Tube 1 OutletVvalve F2 Accumulator A 2 5 mm C
28. HiTrap 1 16 PEEK AKTApurifier 0 5 mm i d PEEK 0 25 mm id F PEEK LiT Tricorn RHESOURCE 1 716 M6 1 16 18 3858 01 p M6 18 1112 55 G i 18 1112 58 1 16 M6 18 111255 AKTAdesign B 18 1112 57 18 1112 55 HiPrep HiTrap HiLoad FPLC HR M6 18 3858 01 _ 18 1112 58 18 1112 57 18 1112 55 18 1112 55 py a HiTrap Union M6 female 1 16 male PEEK 5 18 3858 01 Union 1 16 female M6 male PEEK 6 18 1112 57 Union 1 16 male M6 female PEEK 8 18 1112 58 Union 1 16 male Union 1 16 male PEEK 2 18 1120 93 Fing
29. Reset Zoom 49 8 7 3 1 View 1 Panes 2 Flow Scheme 3 amp 4 8 7 4 Logbook 1 View Panes 2 Logbook 3 amp 4 Resultl i
30. Method a I s m aw w wama m p s bomn ba deee ma m m m 8 System Control 8 1 0 45 nm pH 8 2 1 2 6 2 Load 3
31. 4 3 209 1 Pressure limit Manual Alarms amp Mon Alarm_Pressure High Alarm 2 209 Manual Pump gt Flow gt 1 2 1 4 3 3 UV Cond Pressure END HiPrep AKTA HiPrep 0 15MPa
32. Instant Run F Logon Logoff UNICORN Logon Logoff Method Queue Method Queue Editor Display running Method Queue 1 1 2 2 2 1 C y UNICORN Manager e Method Editor UNTIT QP System Control 1 4 Evaluation 2 Ru open Rename COPY Move Delete Copy to External 3 ClosSe BEIE 2 a 4 5 5 1 Manual Manual 5 2
33. Wizard Fraction order 4 Gradient gt min ml CV Te w et Ee w m FD wap E m EEk EEF E al i a aa hFa l mam Eere r ae I Emer eki Iiei me 1_ mm 41 7 12 File Save As Method Name Technique OK 001 Result 7 13 UNICORN Manageer LINICORN Manager e Method Editor LINTIT a System Control 1 amp Evaluation
34. End Method 2 w save partial result 0K 8 6 i Fa Tisi a wasa mama sa m uma l View Panes 2 Rundata Curves OK Lin ET d animu 3 amp 45 8 7 8 7 1 Run Data 1 Run Data 2 Properties Run Data Groups Fi Fr in Cire bhi anadi Daka i kam Z aan Fa Di Dn pn Fiar ua Caia Cura Wian Dre eoar S r er w Han niia pus iha H kii Akan Ursi Vima A Tiea Eiai F e Fim lt EnEn lm ja UP TE Emuss 1 mH TE Li FT E T a a i PC 3
35. 12mm 18mm amp 30mm _96 L EL EL LE LLL LL EE LI L LILLLTTYEITITTI 1 30mm i 18mm amp 30mm 2 mlmin DropSync 2 mlmin Accumulator Rack A 18mm 15ml Rack B 12mm 5ml Rack C 96 2 Rack D 30mm 50 ml 1 2 Greiner high 780201 Nunc low 156545 Greiner PP Masterblack 2ml 96well 780270 6 15 Accumulator pH
36. Next gt Segment 1 2 Elution Segment 1 2 Page 2 2 m a L w a Segment 1 Wash rm Flush i L Wash Flush Segment 1 Wash Inlet A A PumpWash 1 37 Wash Inlet B B PumpWash 1 Flush System with Set Concentration B 5ml 1
37. Execute F Fuga IH g m u b m g pm s m C EEE iay Fa ku mim 1 1 E amamni i aasan QJB I xE EE sh k 2m 1 r i kum im L pe ria p eo TI NH FT SNP Qd aa QaQunuma n Km rumi d 51 9 1 1 Evaluation 2 File Open Result 3 Open Resut OK PE RW Piere N loirg Bapuk 2 cpp P za i IJE campis P esutDO4 E xampa He Wi 9 2 1 2 Properties 3 Chromatogram Layout 9 2 1 1 Chromatogram Layout Curves 2
38. Pump A Inlet A A1 Pump B Inlet B B1 Next gt r k jl 280 nm 254 nm 215nm 7 4 Equilibration x Start Concentration B B SEE 0 B A Equilibration Volume CV wass i 3 5 CV Watch Equilibration Conductivity pH UV OFF Next gt 7 5 a Injection Technique Manual Pana em System Pump
39. 5 OK m 48 X XX Time min Volume ml 1 Curves Properties 2 X Axls 3 Time min Volume ml 4 Total Window Total Window 5 OK Ab 1 2 3 4 Undo Zoom 5
40. Systems system 1 9 OK Copy from External zip Copy zip 65 12 5 1 UNICORN Manager Methods Results 2 Ctrl 3 Delete 4 OK 12 6 Rename 1 UNICORN Manager Methods Results 2
41. A1 A2 B1 B2 4 1 4 A1 BI 2 A 3 6 re En Ea mx Bs c EFFE I Sus EL 10 ml A 2 lt 4 A1 s 4 B 2 A2 B2 Manual Flowpath PumpAlnlet gt A2 gt A1 A2
42. bl 45 GIZ S eiieeii EEE EE EENE n EEE Are 46 8 88 _ 51 9 FJM 2U gt F722E5F 52 9 1 6a a 52 Je DA eaa EE AA AR 53 99 0 ED oi 56 10 ZZA7 ZTCVhA WT aie ee a 57 Eo 57 10 2 JAT Ad 407 25 09 ee lt F O3 222 752225 ADA h u am osa ER 57 10 4 59 Ll 2227 SA E E E 60 tiel ZAT LOT RN A isa 60 11 2 i 60 NS ALL AN 61 DAA DVIA 61 LLS ae 61 11 G FE u ieta ina 61 12 62 12 20VE Z02237 x uu uum orassyscropapupkaphupa 62 12 2 Copy to External 63 12 3 UNICORNmO 2 Z CDIC3TE 3 25 63 12 4 Copy from External Rt ki la 65 12 5 2741 mGOGHBIBR r 66 12 6 Rename creciera rreren 66 KE zuo AKTApurifier
43. FE 4 Format 5 Stat 6 OK 7 Format complete OK 8 Close 9 My Computer X 12 12 2 Copy to External 1 UNICORN Manager Methods Results 2 Copy to External Epy to litini gs ls h ani i gn Fa e TRT Ci cit eri nj bii Cee 3 Copy 3 1 2 Floppy A Save Copy to External Result zip zip Copy from External 12 3 UNICORN CD 1 CD
44. i Gu kamaq PE Es siwa m o j g z e CN S B y3 OnE HP ORENA O EAE OL NE O u lt fra AT
45. 4 B B2 Manual Flowpath PumpBInlet gt B2 5 A A2 Manual Pump Gradient Targeet0 B Length 0 min Manual Flowpath PumpAlnlet A2 gt 6 End A1 A2 B1 B2 0 05MPa 3 5 PumpWash 1 System Control 2 5 Manual Pump PumpWash Purifier ON ON ON ON gt L ma um ma i L PumpWash Continue Pause Continue PumpWash
46. 3 10mm 8pl 2mm 24 EMITT aAa IFT pH C 900 PH 6 Frac 900 95 10 18 mm 175 12 mm 40 30 mm 3 Frac 950 XY 4 A 120 18 amp 30 mm B 240 12 mm C 96 4 30 mm 8 D 45 30 mm Frac 900 Frac 950 V1 3 7 Load
47. 3 Accumulator PEEK A E 2 mlmin Accumulator 1 OutletVvalve F2 Accumulator B 2 25 cm PEEK 5 mm C TREE ERARI REEE RRN im A ehh UAU EELEEEEFPLET LELKE I s in L 6 13 Keying guide Knurled screw T t 23 6 1 4
48. 3 Pump Wash Manual Pump Flow 5ml min gt 4 5 END 11 2 1 209 2 3 4 13 11 3
49. A1 B1 BufferPrep A1 Buffer A2 Acid Base B1 Water B2 Salt 19 Column Column Sample syringe 5 1 1 2 lt lt 6 2 3 3 4 5 0 7mm 90 5 2
50. Column RESOURCE S 1 ml L Flexible Flow Rates 3 Variables Flow Regulation of the System Pump Flexible Flow Rate BufferPrep p ON OFF k Next gt al 7 3 OR w A WaveLength UV1 1 1 WaveLength UV2 2 pm WaveLength UV3 3
51. 30 mm Start at Fraction Volume 1 ml Tube Type 18 mm Start at First Tube Next gt 7 6 2 Frac 900 Wash Out Unbound Sample C Wash Out unbound Sample 2 CV 2 CV Variables 7 10 Flowthrough Fractionation Frac 900 OFF OutletValveF3 La e OutletValveF3 Frac 900 Fraction Volume Next gt Elution Fractionation Frac 900 Frac 900 OFF OutletValve O
52. X Y 4 X Y 5 Marker 47 1 Curves Properties 2 Curve Style and Color Fk Dua Dama ii Tipi raga Dia Diura um Hii r Caia Tea pema Du Daia LO j w 3 4 OK Y Y 1 Curves Properties 2 Y Axls 3 Fixed 4
53. Fraction Volume 0 First Tube Segment Segment1 First Tube OFF 10 ON UV Cond pH Frac G00 Wizard Elution Technique Gradient Segmented G
54. ConcB 5 Fraction Volume Segment 7 6 1 7 6 2 Fraction Volume Wash Inlet A Wash Inlet B Flush System with Set Concentration B 5ml Fraction Volume 1ml Next gt 7 9 Segment 2 Segment 2 1 Segment 2 2 um rl F Jam Elution Segment 2 2 Page 1 2 0 Step Pump Inlet A and B A1 B1 Target Concentration B 100 Length of Gradient 3CV Next gt Elution Segment 2 2 Page 2 2 Wash Inlet A Wash Inl
55. Flowpath Manual Flowpath 5 3 Pump A Inlet Manual Flowpath Pump A inlet 5 4 A2 Manual Flowpath Pump A Inlet A2 5 5 Execute B Eaa a mi LP pa dl E Damme am p ae S T roma mm s w k System Control Manual 3 1 W1 5 W2 4 W3 1 F3 PEEK 4 F3 3 2 20
56. wa hF Ta 3 OK E Bi mr lir pu ni a Pus lam 93 9 2 2 Y 1 Chromatogram Layout Y Axis pl m L PP pE mm HI hi si sa Pea amam Emi TT Fi Em ma H a Ll OM oan i e o T ET t Le rem Ini fir he EE bl Pi r m Ii pami F T F E FE Lr ai e T m H IT ii a pal B was Fl eF Fe jrr T mi ep bh Felis Hi Fuss a j EE a k Dwe Djs ha ja iama EE LE 1 pal Tra Fyr Macmum e m O Ege is s e Kina J Phis 2 3 Auto Fixed 4 3 UV All with this unit 5 OK Xh 1 Chromatogram Layout X Axis mp al iare Thin rei lam LE masa F Bs 5 1 a s sm amami rm am Fea aa Ea F m mis E Dol mik i wi g i ww i ml TI T m er Kuwa Fest 2 X
57. Manual Flowpath PumpAlnlet gt B2 gt B1 B2 8 2 4 A2 B2 1 A1 3 4 UV 1 System Control Run Data Curves 2 A A1 Manual 1 Pump Flow gt 5mMmin gt Pressure 3 B B1 Manual Pump Gradient Target 100 B Length 0 min eee Pressure
58. System Control Run data Instruments Ready CU 950 LED Power PC System 3 2 22 UNICORN 2 2 1 UNCORN UNICORN 4 UNICORN Manager Method Editor System Control Evaluation 6 A UNICORN Mariager e Method Editor UNTIT QP System Control 1 4 Evaluation F UNICORN Manape UNICORN Manager Copy Rename Delete Move MethodEditor System Control Evaluation iian
59. 4 ml min 100 HI 100 ul Segmented Gradient Advanced 50 B 10 CV 280 nm 254 nm Frac 950 Frac 900 7 1 Wizard Method Editor File New New Method Wizard OK 30 7 2 UT waw I Main Selection ZT ZIT TQ SK uM Affinity Anion Exchange Cation Exchange _ Chromatofocusing CIP HIC Installation test RPC Size Exclusion a Cation Exchange
60. PEEK pH Cell PEEK a mm ul 10 cm HR PEEK 0 25 4 91 PEEK 0 5 19 6 AKTApurifier 29 Wizard 1 Wizard 2 Wizard RESOURCE S 1 ml
61. END o7 Frac 900 gt 1 Manual Pump Flow 2mMmin gt 2 3 Manual 1 Frac Fractionation 900 FracSize 2ml 4 Manual Frac Fractionation Stop 900 5 Manual Flowpath OutletValve gt F3 gt 6 3 END 10 4 J 2 UNICORN Manager File Quit program UNICORN Manager YES System Control Leave Control of System1 OK Windows Start Turn off Computer Turn off Turn off computer
62. 1 CU 950 2 AKTA ON PC OFF ON 3 ON Login Windows 4 Windows UNICORN 5 Log9on User name default Password default 6 AKTA UNICORN Manager 6 AKTA
63. CD 2 OK EEA EATA UEP S H QB su Rumi H HKG T sama LB 3 CD g G Delete temporary files 63 4 My Computer CD UNICORN Result C UNICORN Local Fil CD amp C UNICORN LocalFiMDefaut Result Manual Runs ieina hl Pi DI PPT Mirr Yum ma i h r mi rr rar TI 7 Finish onmi BL mm g cfmvimwn Tran C PT rm Hi rt i EE ri Mi E PEEM M p T u E nE SE m Nre rE mmp 1 T S D jema Te mem mmm
64. Direct Loading Manual Empty Loop With 2 3 1 2 1 Next gt 7 6 7 6 1 Frac 950 Frac 900 7 6 2 Frac 950 Setting Flowthrough Fractionation rr SAN m Frac 950 Rack Framak ta 18 amp 30 mm Tube _ Wash Out unbound Sample 2 CV mea gt 2 CV Variables 7 10
65. Err 12 12 4 Copy from External 1 UNICORN Manager Methods Results 2 3 Copy from External 4 Copy 3 1 2 Floppy A RI iaie nal Copy g iH Pmr i 1RTF ma D EE D a se Paf bie om a CCORI DES FE T ml Ed Teri Ekm Oo tees 5 OK 6 Result Results 7 Method Method System Connection H RE ET E TE EE fd F sa He i S Ha mW pas h pe bw J 8 Method file
66. Gradient Advanced Gradient Segments k 2 Reequilibration after Elution 5CV 5 CV k Next gt 7 8 Segment 1 1 2 Segment 1 1 Elution Segment 1 2 Page 1 2 a Step Gradient usai Gradient J Pump Inlet A and B A B A1 B1 Target Concentration B Segment B 100 i Gss Length of Gradient Segment CV 20CV w RE SH Gradient ini Pump Inlet Aand B A1 B1 Buffer Valve A Inlet A11 AA Target Concentration B 50 Length of Gradient 10CV EAI LE Ta
67. Page http www gehealthcare co jp lifesciences 06 10 5 CGP GE Healthcare r gt N FO C A di H gt O H ell JS lc UZ 6565SNSIB OIEE lt 2E C BEEK ADIBE Os DR win usu ORAAL JALAN
68. gt OFF En ESE OFF ESA 39 IE 11 1 2 20 209 20 pH pH 1 209 2 System Control Manual Pump PumpWash Purifier ON OFF ON OFF
69. 5 5 y UNICORN I KTA UNICORN B xW QSX z KTA qAq 65 gt CU 950w Powerq PCz System bU n 5 Input 24 32V 1A SM wm TM OtmM o y Frac 920 w M Oxz Frac 901z 900 q pb k v Frac 901z 900 w MXi M B6S amp AM GB 1 0 y Frac 920 xop pV iha lt t agQ OM mM oM Uz qw k U ist A tx Setup and Check 9q E b AKTA zpw UNICORN T iw M Q sb Qm 70 KTA sk O Pw 0000 AB VMfgt WW CDM tfp gt o Properties 6 R amp Display Properties q U g bwpz Screen Saver o Power 66 Bt Power Options Properties 4 EU wp Plugged in W lt w System standby 6 Never tIAB 0 M whg qU K Bf UPC 9004 AKTApurifier UPC 10 100f6M wM A fi WR 1y hM WR BI 280 nm p 254 nm p I 2y Hg v Y gt Qz 280 nmq 254 nm xB W 1 q 36 fi GE bfwE UV w OFF tb 1 yaW UPC 900 wm8Astg 5 5 2y Lamp ON OFF w U ph m OK 6z OFF R omw OK 6B Lamp on Lamp on on off UPC 900 wm UpVsM xz r System Control System Settings Specials gt Keyboard Modep Open t OK 0 b ee GE 169 0073 3 25 1 ISO 9001 2000 TEL 03 5331 9336 FAX 03 5331 9370 e mail Tech JP ge com Home
70. GE Healthcare AKTApurifier xa S wM A GE imagination at work UNICORN Ver 5 0 UNICORN Ver 5 0 PumpWash Purifier Continue PumpWash Pause Continue Frac 950 Frac 950 2 Manual Flowpath OutletValvei F2 Manual Frac Man_Fractionation gt gt Frac 950 Wizard COX Y w NERIS Elution Fractionation Start at First Tube amp L Segment1 Fraction Volume
71. a waq mw awa 22 S a C D E E E E N E E E N E 26 255 ua EE A A E 30 7 1 X v FfERk WizardIBIIBIlOSSR 3O 7 2 J03 P2374 RE DILERE 31 7 3 32 7 4 Equilibration 32 Z5 eiri 2 uu ul Q haaat 393 Z6 JIJ 2a IJV 2 QDBSE sassssssssasassss a 33 Z ADDA Elution OGG 36 7 83 Segment 1 37 7 9 Segment 2 39 10 VariablesIB i8 COAJA B El 40 7 11 Run Setup AT 7 12 XJ Y FORT siiryi niran aa ANNEN 42 9 ROAY YTO R er NN 42 8 s assssasss 43 4 22 saus a saasakasashanaycuqpayqaq han ETEN EANAN ANNENS 43 8 2 L 2 G 35 2 J DSBIR uu uuu uuu nais 43 8 3 E KRKKKMKNKNNK W VCIlrir 44 Pad E E E Eo 44 SIT 45 8 6 KEKKRKKOKEKE EE
72. ertight connector 1 16 male PEEK 10 18 1112 55 Stop plug 1 16 male PEEK 5 18 1112 52 Union Luer female 1 16 male PEEK 2 18 1112 51 SRTC 2 M6 female 1 5 mm i d PEEK 5 19 2143 01 Tubing 0 25 mm id blue PEEK 2m 18 1120 95 Tubing 0 50 mm id orange PEEK 2m 18 1113 68 Tubing 0 75 mm id green PEEK 2m 18 1112 53 Tubing 1 0 mm id beige PEEK 2m 18 1115 83 17 4 2 HiTrap HiPrep UNICORN Manual Alarms amp Mon Alarm_Pressure High Alarm 1 Manual Pump Flow 0 5 ml min gt 2 UV 3 END
73. et B FIIL Flush System with Set Concentration B 5ml Fraction Volume 1ml Finish 39 7 10 Variables op Ena m l mmm Ea Cama A Fi Wizard Variables Value Variables gt Wash Out Unbound Sample Reequilibration Flexible Flow Rates amp Variables Show details 7 11 Run Setup Frac 950 gt amr a Foam all sopes mE kam zmier HFF UE T e ia berer r er Mea ETT iE bhes Bing ka pre a I Err BE H rhe Rack
74. ig ram f ml mm P prse N FK rena am in e a gsl F phg J rum Ec Pharma or em Ee i hm UI me aa p i ma Le i J ar Mion i E E i a E i rik TY mam masqa K TRay Aq 8 IF Hek Fimi rl L Im kii m F 8 8 System Control Run Hold Continue Pause Continue Pause Continue Hold Pause End 1 Manual
75. radient Advanced Segmented Gradient Advanced Contents 1 ADT o 4 1 1 AKTApurrifier 4 12 AKTApurifier 8 oe 9 2 1 UNICORN 9 2 2 UNICORNOJSMETE S asas tt 11 13 S 41 J amp I S 2085 EE 13 SE CRE ii 13 59 MAIDAN CI IS eaer Tii 14 24 TP ETIIGO TS u i EE TEEDE i 15 35 Jh2Z 5 s PUPRDWGSKD oo humuqasqisqa 15 oe 16 J 3 L O IK IT uuu usus a CE 17 Al J mj l22 J Ei 17 4 2 18 43 JI LOKEM ie 18 aA J u 25 COAN uu u uu ulus ils buQassuass 19 2O 5 1 20 5 2 21 i 22 O l PI C O O i puk
76. utletValve Frac 900 Frac 900 3 Fixed Volume and Peak Fractionation Fixed Volume Fractionation Peak Fractionation Fixed Volume Fractionation Fraction Volume 1 ml Next gt 39 7 7 Elution Elution Technique lsocratic lsocratic lsocratic with Delayed Fractionation Linear Gradient 1 Segmented Gradient Advanced 9 Step Gradient StepWize Gradient Segmented Gradient Basic 9 Gradient Segmented
77. w ml min Execute 0 15 0 35MPa va VAT LERE UL No 0 15MPa F Check 2 0 35MPa Act 2 Gradient Conc B O0 InjectionValve Waste Execute 0 10MPa 0 InjectionValve Yes InjectionValve Act 3 11 3 69 CU 950 0 PC B n sM y CU 950 q Pw USB oX i M 6 CU 950 w9 8U6 IT 8gMfOCoXi M System n sM y Uninet z i AU CU 950 w Test UniNet 1 tAODM f 818704 HZbM wp 6 Froction Collector Frac 920 0 Network p x s X fi CU 950 w CU 950 x AKTA w f w fk iqU pV B 1 h KTAU ThttKTtx Pt 60 9wt t 1 y Uninet A KTA CU 950 6 Uninet N 2 4x KTA q CU 950 w oxWBb 2y USB CU 950t USB AM00 Pw USB fUW USB Mio6B1 3 y CU 950 w oO Zo CU 950 o 5 o t 5 3E CU 950w Power nz PCq System x b KTA T P o AoM T xz w p lt t Bi 4y KTA q W oO KTA w oWOB Zo90 W 2oWO5I Windows UY4Biq0B CU 950w Powerq PC nz System x B PC n sM x BI
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