ReproFF2TM
Contents
1. 1O IO gt For Research Use Only po ReproCELL A Vitronectin N gt A1 A2 A3 Vitronectin N 1 5mL 120 LL 80 lt 60 mm 4 gt A4 A5 AG A AS 15 mL PBS 8 mL PBS 8 mL 120 uL Vitronectin N 67 60 mm 2 mL 1 4 1 B ReproFF22. Q3 A3 4 Q4 A4 1 1 https www reprocell com E mail info_jpQreprocell com WWW reDrocell com For Research Use Only
3. PBS C6 ReproFF2 2 mL 5 mL 15 mL C7 ReproFF2 2 mL ES iPS 15 mL C8 5 mL 2 5 mL 1 200 300 pm 2 WWW reprocell com For Research Use Only po ReproCELL 5 2 lt
4. ES iPS 60 mm PBS 2 mL PBS GCDR1 mL 37 CO gt 3 GCDR PBS 2 mL PBS ReproFF2 2 mL ES iPS 15 mL ReproFF2 2 mL ES iPS 15 mL 5 mL
5. 0 lrrzAl 1 1 Mc 2 2 MC Mc 3 3 Mc Mc MC 20 2 8 20 2 TE iPS Takahashi K et al Cell 131 861 72 2007
6. WWW reDrocell com pH 2 Mercaptoethanol ReproFF2 ES iPS ReproFF2 5 ng mL bFGF RCHEOT002 003 5 ReproFF2 ES iPS bFGF Gentle Cell Dissociation Reagent GCDR STEM CELL TECHNOLOGIES 07174 Vitronectin N LIFE TECHNOLOGIES REFA14700 PBS Ca Mg T free PBS 60 mm P 1000 GCDR Vitronectin N 4 Laminin 5 RCHEOT004 Corning ES Corning
7. 60 mm B2 B3 B4 B5 B6 B BS 1 ES iPS 30 Fig 1 2 1 2 7 ES iPS 3 500 1000 um 1 ALP A ReproFF2 2 mL
8. 2 5 mL 1 1 200 300 Hm 2 4 2 2 200 300 um 250 um B9 B1 B10 37 CO gt B11 ReproFF2 0 lr l 1 Mc 2 2 Mc Mc 3 3 Mc Mc 4
9. 1 1 2mm MC C 60 mm C1 A ReproFF2 2 mL C2 ReproFF2 PBS 2 mL C3 PBS GCDR 1 mL 37 CO gt 3 C4 C5 GCDR PBS 2 mL
10. C9 C8 5 C1 4 1 2 4 1 3 1 4 C10 37 596CO gt C11 D Q1 3 85 A1 85 Q2 A2
11. a 8 ReproCELL ReproFF2 Cat KCHEMD006 Version 2 0 Gentle Cell Dissociation Reagent GCDR 200 300 um 7 500 1000 Um ReproFF2 4 1 2 bFGF 10 30 ng mL lt ReproFF2
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ReproFF2TM