"取扱説明書"
Contents
1.2. 2 0 3 5
3. 51 3 3 1 q 8
4. i op 43 2 2 1
5. 45 1 pH ml g dry gel Da mm Sephadex G 10 2 3 700 55 165 2 13 Sephadex G 25 4 6 1000 5000 38 235 2 13 Sephadex G 50 9 11 1500 10000 38 235 2 10 Sephadex G 75 12 15 3000 80000 59 285 2 10 Sephadex G 100 15 20 4000 150000 100 310 2 10
6. SDS PAGE SDS PAGE 3
7. 2 0 30 46
8. 41 1 3 g 5 1 12 x rn lt x 10 nhn rpm revolutions per minute cm
9. 2 2 NH CNBr Sepharose4B Amersham 0 pH8 10 4C
10. 1 B C18 B Si CH CH CH 1 A B C 2 1 2 1
11. E 44 pH pH
12. 2 NR 3 3 3 Kb DNA
13. LC J HPLC 10kg cm 1 FPLC E
14. 2 48 2 3 1 A Si OH
15. Gel filtration
16. pH 53 l 0 C C1H C1 1
17. 1mm 1 3 2
18. pH A E 3 E pH 0 pH H OH NaOH OH HT 3 OH
19. 52 fY Or Davis 1 2 pH 6 7 pH 8 9
20. discontinuous discontinuo us SDS PAGE lt RNA DNA
21. 1 1 2 3 57
22. FPLC HPLC 10mM pH6 200 500mM Cu Zn T Ni Ca His Cys Trp
23. 2 A 2 B A B YY ON NT 2
24. C18 HPLC m m A HPLC
25. HPLC 1 1 50 HPLC HPLC 2
26. dd _ KD 500 NO 3 1 2 SDS PAGE 3 4 10B UV 3
27. A
28. SDS SDS SDS 54 SDS PAGE 0
29. pH8 3 7 ii HCI pH6 7 pH8 9 1 2 A C1 pH6 7
30. C1 A B i NN CT Pro 1 Pro 2 Pro 3 Cl WN ws OO 2 Pro 1 2 3 8 9 ES 2 B pH EL Px
31. 55 SDS PAGE A B A 1 A 2 B 1 B 2 B 3 0 DNA ON Ot A ON
32. pH HH pH E GO 1 pH H pH
33. pH pH pH pH pH PH pH pH
34. K Ka lt VJ V V V V KJ V V DNP pH 0 2M SDS
35. 1 2 5 3
36. H Y qH q 2 1 EA E qgH F F Tiselius
37. 15 000 rpm 50 m 500 mL rpm 150 000 rpm 1 2 2
38. 49 3 A 3 B 3
39. pH pH Ni N His His 2 2 2 1 1 C DN
40. RI 9 0 SN 56 RI SDS DNA RNA
41. cm g rpm a 5000 50000 7000 700000 6000 600000 4500 45000 5000 500000 4000 40000 4000 400000 3000 300000 3500 ssooo 2000 200000 3000 30000 1500 150000 1000 100000 2500 25000 800 80000 700 70000 600 60000 s0000 2000 20000 40000 30000 1500 15000 1400 14000 1300 13000 1200 12000 1100 11000 1000 10000 9000 8000 42 1 4
42. HPLC mm 15Okg cm 280nm pH H
43. pH 10 100mM CM Carboxymethy1 0 CH COOH SP Sulfopropy1 0 C H SO D DEAE Diethylaminoethy1 0 C H N C H 2 QAE Quaternized aminoethy or Diethy1 2 hydroxypropy aminoethy1 0 C H N C2Hs CH CH OH CH pH4 5 pH7 8 5
44. C 47 ATP NAD Ca PO OH 2 Ca PO4 1 lt
45. 15 2 ECH Sepharose4B Amersham pH4 5 6 4C 1 5 24 CH Sepharose4B Amersham pH5 10 4C 1 4 COOH EAH Sepharose4B NHS Sepharose4FF Amersham SH Thiopropy1 Sepharose6B Amersham Thio1 Sepharose4B Amersham pH6 5 9 4C 1 Po to 5 2 pH4 8 4 3 16 pH4 8 4 3 16 Amersham 10 pH4 5 6 4C 1 5 24 NH SH OH Epoxy Sepharose6B Amersham 12 pH9 13 20 40C 16 3 Con A Concanavalin A Cibacron Blue Procion Red Heparin A Frotein A Streptavidin Lectins 3 NAD NADP NADP IgG anhydrotripsin
46. 5 1 1 1 50 5 000 rpm 15 000 rpm 2 mm
47. P G 50 DNP C G 50 5ml 2ml G 50 5ml 2ml 5ml 0 2ml 0 2m1l 2 5ml
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