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1. Gallios 0ne Day Gallios Gallios Ga2. 1 3 5 EE Ge Ph a ec Taibo PR Tentr allien FIL ans FR 10 Gallios 4 10 1 0
3. Gallios DRAQ7 CD25 PE a 3 La CD3 PC7 CD8 BV51 g A Tn RE 1 a L 1 L fia i 1 ME i td ft EE A A EN 4 be Pr n FE L t Ja 1 F i O LE a F i 90 PE WW CD62L BV42 1 CD4 FITC D Gate 71 9 1 CD62L BV42 1 CD62L BV421 6 FL1 CD4 FITC FL2 CD25 PE FL5 CD3 PC FL DRAQ FL8 CD6 L Blliagnt Violet 421 FL10 CD8 Blliant Violet 510 DRAQ7 CDG CD25 Treg WM CD4 CD8 4 CD62L ET _ G2Phase Sa 22 5 FE i EE a CN lt EE amp oS Single Cell M Phase 5 26 7 i 7 AAD PEAK 7 AAD Jurkat
4. NN 10X10 10X10 Gallios 1 Thermo Electric Controller 5
5. Gallios Quick Comp PRTIDaRTWWiPm RN a a Fr Le i a Ei RIwmtcetiwiiew _ III FL2 INT LOG FL2 INT LOG FL1 INT LOG FL1 INT LOG QuickCOMP QuickCOMP APCAF7DO APCAPY5D Pu mf Fa i ai 5 sg F a 1 4 True View True View LMD Quick COMP oe
6. Gallios iss iam NM Woekc Mr 2 Cytometer Task ee hd Cytometer Task eo yea Galliosg ek Hm 3 Gallios 32 3
7. Ti BBW E LE lt Microsoft Excel PDF Microsoft _ PowerPoint ECA LOLETER 2013 320 ee 0120 826 77 7 03 6745 4705 9 00 lt 17 30 GMP GLP
8. G TrueView QuickComp Singlets 1 0 61 Singlets J 0 45 Singlets Singlets 1 H 071 a CL oO 3 2 8 3 9 A A ER 1 RE FS A FL10 DAPI Log FL1 CD38 FITC FL2 CD133PE FL1 CD38 FITC LinP LinP CD84F Clean CD3 io E 90 06 G 0 72 EE 6 D 0 07 F 95 56 EE 9 8 FS A FL8 CD45AO7 FL3 CD34ECD FS A FL1 CD38 FITC CD45N Ln LinN CD3 L L et M2 100 8 Ni 16 o 01 98 ss i L 85 56 mW Il fa a 8 W 8 1 1 XY c i M3 0 00 M4 0 00 LL M3 74 84 M4 6 69 03 0 00 M4 0 00 FL3 CD34ECD FS A FL1 CD38 FITC FL2 CD133PE FL5_VEGFR2PC7 Channel Fluorochrome Specificity ei FITC CD38 Committed Heme Progenitor w CD34 FL2 CD133 Heme Progenitor Subset FL3 ECD CD34 Heme Progenitor CD14 33 20 3 8 FL4 PC5 GIyA Heme Lineage 5 BS PCZ7 VEGFR2 Endothelial Progenitor ES APC CD31 Mature Endothelial FL7 APCA700 CD146 Endothelial Progenitor FL8 APCC7 CD45 Leukocyte FL9 DAPI Lin DNA Cell cycle 0 DAPI Log DNA Debris Circulating Endothelial Cells
9. GalliosS ADC 4 000 Gallios G5 000eps Gallios ADC 20 40MHz 60 096 Enor 0 40 30 18 10Mz O m 20 SE ON 10 0 40Mz DSP Log Decade Display A Gallios 18Ew wk TOMz DSPOO 0 40Mz DSP DPS Gallios 20 ADC 596
10. Panel A Panel B Panel C E coli Cherry rm 1 fm 1 1 F rm Im 1 FL2 582 15 FL2 582 15 FL3 620730 Panel D Panel Fr we i FL2 582 15 FL3 620 30 488 561 nm A D mCherry dsRHed mKate dTomato Gallios FL 1 FL E FoxP3 mHFP blue Gallios FL FL9 Gallios TEC kawa9 27 cellcycle Pl stnd 01 A FL3 Lin HST Cell Number i PlLin DNA Content Jurkat PI Gallios MultiCycle for Windows
11. W2 3 1 8 8 198 1 8 Wa 8 19 1 19 3 ca This Dimen ER i a of Lens ni er Plal Rd Photo Sensitive Devices Ge Si Sing In nly o Focal Length of LL Lens 1 unit New 561nm 56 1nm PE PE mCherry DsRed dTomato mKate 56 1nm PE DsRed 56 1nnm New 56 1nm 56 1nm 488nm 809 561nm 488nm M APC APCAF700
12. 25 000 Gallios GallioS TEC Gallios AutoBeam AutoBeam
13. EE PS AE a EE 6 or a0 CO lt Cick to add bars gt 2 4 Tree 4 1 6 Tree 1 Cowunt 3 es Gallios 4 10
14. 1 BB ee Ee Pacific Blue 488nm 3 638nm 1 405nm 1 1096 5 5 1096 4 Gallios 10x10 QuickComp 4 TrueView
15. lsolation and functional Droperties of murine hematopoietic stem cells that are replicating in vivo Goodell MA et al J Exp Med 1986 183 1797 1806 Side population analysis using a violet excited cell permeable DNA binding dye William G et al Stem Cells 200 25 1029 1036 Gallios 3 Gallios 1 10 4 0 10 W em emm SS PEAK mCherry DsRed dTomato G Gallios 56 1 nm
16. Gallios FCM A FCM B Gallios Blue loss Red loss MM CE YellOW IOSS Blue power Red power Violet power Gallios Gallios TEC Thermo Electric Controller Gallios Gallios TEC
17. MD Fi D1 FE OD I 1 FS FL PL2 PL3 FL4 FL5 FL6 FL7 FS FL9 FL10 TEC 3 Gallios 1 19 1 8 W 8 19 3 8 40 m W 0 5 10 m Gallios Flow Cell 0 5um NOISE SS 0 4 m FS 0 4 0 5um Gallios 0 4 m 0 1um
18. Gallios 18 Boulevard Gallios ADC 20 40MHZ TS 2 18 Boulevard 77 eps 5 100 eps J ll MM 1 FL1 Int Log FL1 Int Log FL1 Int Log 10 239 eps Count Count Count 30 6 eps Count Gallios 4 000
19. Cyclin A GO M G1 S G2 AAD Cyclin A2 FS DNA Cyclin A2 10 11 Kaluza Kaluza Tree
20. A 135 0063 3 5 7 TOC g 0120 566 730 http www beckmancoulter co jp CE9904 2013 12 2000 E Gallios NEW MODEL Gallios 4 Laser System Powerftul Versatile Performance Fast Irack to Success WE A New Frontier tn Flow Cytometry alIIOS High End Flow Cytometer ios 1 0 3alli 8
21. 405nm 56 1nm 561nm 150X480 m N A 1 8 0 4 m Boulevard FITC 112 MESF PE 78 MESF PC5 15 MESF APC 75MESF 25 000 25 000 40MHz 0 025 20 1 048 576 3 amp si sip 50 ge WW rir Reser 2 0 se i EE 32 4 10 Windows Vista 953 W x701 D X605 H mm
22. Boulevard Ferrm has A et I I Ln Ln Lg Ln i rr mmml es me mmm smsm mmm mm mmmi mmmr mm Fe 1 EE 1 mm ss _ Ok cm me 16 Gallios Am 2 9 mm ae FITC 488nm 4 638nm
23. 10 Albert D Donnenberg Ph D Professor of Medicine Director UPCI Flow Cytometry Facility UPMC HSCLab SP SF Gallios 405nm Vybrant _ Dye Cycle Violet SP m m We SD gt SD SS SS gt gt JO a me gt gt a TR LE TA TARY A A EE DyeCycle Violet Red DyeCycle Violet Red SP SP Side population Hoechst 33342 Hoechst 33342 UV 405nm Vybrant Dye Cycle Violet Life Technologies SP 9 Dye Cycle Violet 5uM 37C 90 Dye Cycle Violet 5uM Reserpine 10uM 37C 90
24. 104Kg 724 W x495 D x298 H mm 30 39Kg 100V AC 15A 50 60Hz 100V AC 10A 50 60Hz 16 32 30 80 Gallios Beckman Coulter Beckman Coulter Gallios Beckman Coulter Inc
25. APCAF750 HTC PE ECD 1 PC7 Pacific Blue Krome Orange f A 1 488 561nm GFP mcCherry PerCP PC7 j Ff Gallios 488 nm ro rs rans ne mg re no Fuo PC5 APC APC Padic Fluorescet dlorrsto Fixabe Protgirs GFP nkale meny eFluor amp Viability dsRed 2 780 Gallios Gallios 18 Boulevard Gallios Gallios Gallios ND 4
26. llios 20 Web FCM WeD http www bc cytometry com Galios z Gallios lt gt 488nm 22mw 561nm 81 5mw 6538nm 25mw 405nm 40mw 488nm 638nm
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