公表特許公報 特表2015
Contents
1. 1 0 Pseudononas OOOOOO000 do O O0Q00000000000000000000000 ao kordaQOOO0000000002. 0000000000000000000000000 19 0000000000000000000000000 20 30 0000000000000000000000000 4 00000000000000000000000 00DDDD Pseudononas fl uorescens Pseudononas sp O 000000000000 Synechocystis
3. O00000000000000000000000 10 000000000000000000000000 2 00000000000000 30
4. ES mun BRYA 2 7 7 37 7 PERET EL FALE oy AAA AYYYAAFRASFAR oc EY BUND 7 KAER 7 lt 37 4 BALE ayyy BE 39VV41 FR 4 713 OC BYARD bhpenay FADE cOLAERSR UR aZUVVXATAUDRTAR SON Eye BYARD 77 better TAN AAB SLE ADYIRAFR 374 mse wen PEP MADLYENPMGLMG nmm MPPTPAT ATIGAAAAAVIPEHAARZ FPRVVRVNPRSDREPVLZEHH MPPTPAT ATGARAAAVTPERAARS FPRVVRVNPRSDRFPVLSFHR MPPIPTAAAAGAAVARASAAEQAAFRLUVGHRHEVRENPRSDREHTLAFHH MTQITHHTPDTARQADPEP MGHONAAVSEHQHRDDGAASSPGFKLVG FSXPVRENPKSDKERVKREHH MPPTPTTPAATIGAAAAVTPEHARP HRMVREHPRSDREHTLSGFHE MGKE QSEAEILSSNSSNTSPATFELVG FHHPVRANPKSDHEAVKREHH MAPGALLVTSONGRISPLTDSDGYVPAP AALVVGGEVHYRGYHH HAPAADSPTLOPAQPSDLN QYRGYDH IBFASPTPGTLEPIFEIMGETEVATHRSKN VHLYRQGEINLIL VELWCADAASAAGRESFALGAPLAARSDLSTGNSAHASLLLRSGALAPLE VELHCADAASAAGR
5. 20 0000000 0 0 Pseudononas aerugi nosa O Pseudonon as agarici JO OO 000000 30 0000000000000000000000000
6. 0 CAnaranthus 0 0 0 0 0 Abrosi a D 0 D CAnoda r OO OO D D D D D D CAphanes 000 0 CA rtenisi a DL D D B U D CA trip amp 0 00000 B den s D Gaesella ti 0 O00 D Garduus Q 0 U DD 95 CGertaurea 0 0 D D O Chenopodi um OOOO G esi unti D U D O Gonvol vul us O OOOO 000000 Btura g Csnod umG Egg 10 CErysi num 0 0 Euphor bi a 00000000000 leop
7. 70 0000000000000 0000000000000000000000000 2 au 0000000000000000000000000 30 0000000000000000000000000 0000000000000000000000000 0 0000000 variants QDO 00000000000 O00Q00000000000000000000000 4
8. 10 20 Pseudonoanas fl uorescens 38 JP 2015 528310 A 2015 9 28 73 74 10 ooo LJ EJ EI 1 1 EJ 11 1 1 1 E
9. O0000000000000000000000000 50 8 JP 2015 528310 A 2015 9 28 10 Pseudononas fl uorescens 0 0 0 0 Avena sativa g0 0000000000 Streptonyces avermti 5 Arabi dopsi 5 thal i Hordeumvul gare Daucus carota OOO 0 Mycosphaer el a granni col a D D UL 20 3 e ET ER 4 cocoogooooooooooooooooooooooi
10. Gcc co au ids OOOOOOUO0O00000000000000 OOO Gris japonica 0000000000 Aanydononas rei nh 50 6 JP 2015 528310 A 2015 9 28 00000 0 000 00 00000 0 0 1 0 000000000000000
11. 0 0 0 0 50 000 0 26 JP 2015 528310 A 2015 9 28 o i 50 27 JP 2015 528310 A 2015 9 28 51 52 0000000000000000000000000 0000000000000000000000000 i i 10 0000000000000000000000000 i i 0000000000000000000 0 operab i D linked 0000000000000000000 i 20 0000000000000000000 8 8 3 28
12. 30 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 transgenic event 24 8 8 5 3 JP 2015 528310 A 2015 9 28 25 JP 2015 528310 A 2015 9 28 N e 00000 D D OOO 10 8 8
13. 4 0000000000000000000000000 0000000000000000000000 00 50 000000 46 JP 2015 528310 A 2015 9 28 89 90 DU DU DU DU DU Oooo Ol Fea E Eel ESI ESL EE O Gonananas testosteron Hd o 1 EVER a E EZ Ez ESTE EX 7E El TV 20 EI EST ESI To ET ER EST ESTERI LE EI
14. 42 JP 2015 528310 A 2015 9 28 81 82 RUE 0 0 Km uM Kcat s PIHPPD 187 44 1 000000000000000000000 P1G336W n 54 2 000000000000000000000 PHPPDEv037 220 30 9 d 000000000000000000000 e Hd o CO210d10 1181 14 CO212f3 297 0 9 10 C644 232 0 5 11 C645 156 0 8 12 c0216C6 1541 1 2 14 c0213H10 489 2 6 15 PfHPPDEvo41 336 11 16 EL Ee zl NJ e PfHPPDEvo37 0 36 m 3 BUE S 30 CO23E6 0 38 Emu CO24H11 0 01 PfHPPDEvo33 0 25 PfHPPDEvo36 0 82 PfHPPDEvo40 0 42 CO210d10 043 9 o ja e
15. 48 JP 2015 528310 A 2015 9 28 93 94 00000000000000000 0000000000000000000000000 10 D 0 00000000000 D 2 AIO 000000 30
16. 0 10 0000000000000000000000000 20
17. 10 jore ir 2 0 30 4 50 34 JP 2015 528310 A 2015 9 28 65 66
18. 4 50 0000000 4 JP 2015 528310 A 2015 9 28 5 6
19. i 50 14 JP 2015 528310 A 2015 9 28 i i i 50 15 JP 2015 528310 A 2015 9 28 o N e 8 OO EF OO OO OO E OO El E E ENE OO ENE OO 3 EE ooo 16 JP 2015 528310 A 2015 9 28 29 30
20. 4 000000000000x00000x00000x 000000 O0000000000000000000000000 50 19 JP 2015 528310 A 2015 9 28 OOOO
21. EZ DLE EL Ed EJ EI Er B VER AE E 1 1 ESI IET ES REE ENE EI EIL EE EN EEI BED E ELLE E EJ EJ EJ E E EJ EJ EJ EJ EX 2 a ar L3 L3 oo 11 Ea EE SES E ra ETT EST EI EL ESL DES EZ E EE BEES ERE EST EST TEST EI LESE SES E EST EE TESI 1 OESTE L1 E E EIS EI E ey EILIJIL LE Ez At ES CEST ESS TEE st ES EZ EB ES ES LEE Ez 3 EE EI E I ET SET ET ESTEE ES LL E e 155 a LI E T 1
22. 000 0000000 0 000 0000 00 0000000000000000000000000 20 00000000000 0000000000000 0000 0 000000000000000000 30 00000000000000 000000000 0000 0 00 00000000 0 0000 40 0000000000000000000000000 50 7 JP 2015 528310 A 2015 9 28 11 12
23. 10 77777 2 7 PEHPPD 6 4 58 58 2n s O0Q00000000000000000000000 ao 0000000000000000000000000 3 000000 00000 0 4 00000 O0000000000000000000000000 50
24. OQ00000000000000000000000 4 000000 Athrobac ter gobiforms OOOOOOO00 00000000 0 O0000000000000000000000000 50 23 JP 2015 528310 A 2015 9 28 43 44 10 20
25. o 1 1 1 12 E EIE E D E ES EISE ES N e 8 EET E jESE aed eds ij AES ESTE ESL Tet TE EXE ES Es 8 3 35 JP 2015 528310 A 2015 9 28 o N e 8 000000 0000 Aegi ops 0 0000 rosti s EE ER E opyron A opecurus Lol i umD LI 0 Nonochor 8 LU D D D Panicum D D U Paspal D 0 DU Phalaris DDD DO DU Ph eung Poa H1 H DO D CFottboe
26. 18 JP 2015 528310 A 2015 9 28 5 0000000000000000000000000 10 0000000000000000000000000 0000000000000 000 0 0000 2 0000000000000000000000000 O O0O000000000 0 00 000 00 30
27. TEE I E ER LEE tf cr EET ES ERE noaoooo Soon CET EJ ES 57 0000 000000 00 00 HU 3 30 N e 8 LT E JP 2015 528310 A 2015 9 28
28. 10 20 8 L1 E EZ EIS EE ry Ea EI ELIO Ld ee EJ Sea Et ea EVE EHSEJ EMOEM E EJ El Ed E 7 LJ LJ LJ LJ L3 L3 EJ EJ E3 oOo ESTE SEXT ESI CEST ET EST ENE ESI EST EET EET E EE EXE ELT EST ENE a sd EET CEST ESTE 2E ET E ET EST EPE EF ESL ESL LEES Ed DLE EH ES Gd EH L3 L3 L3 L3 Ed L3 L3 L3 L3 EJ oo E EJ EJ EJ E Ed EJ
29. O00 O0000000000000000000000000 50 00000000 17 2015 528310 A 2015 9 28 UJ UJ NJ 0000000000000000000000000 00000000 0 0000000000000 0000000000000000000000000 00 0000000 0 000 0000 000 0000000000000000000000000 0000 0 000000000000000000 0000000000000000000000000 00 000 0 0000 0 000000000 0000000000000000000000000 0 0 00 000 00 000000 0 00 0000000000000000000000000 3 0000000000000000000000000 2 0000000000000000000000000 0000000000000000000000000 3 0000000000000000000000000 0000000000000000000000000 4 0000000000000000000000000 5
30. 2 JP 2015 528310 A 2015 9 28 0000000000000000000000000 10 O00 000 3 0000 30 0000000000000000000000000 0000000000000000000000000 40 0000000000000000000000000 0000000000000000000000000 s 0000000000000000000000000 3 JP 2015 528310 A 2015 9 28
31. 10 JP 2015 528310 A 2015 9 28 3 000000000000000000000 2 0000000000000000000000000 0000000000000000000000000 3 0000000000000000000000000 4 0000000000000000000000000 0000000000000000000000000 0000000000000000000000000 0000000000000000000000000 0000000000000000000000000 0000000000000000000000000 0000000000000000000000000 0000000000000000000000000 000000000000000000000 5 11 JP 2015 528310 A 2015 9 28 19 20 o N e
32. 5 O0Q00000000000000000000000 ao 0000000000000000000000000 30 0000000000000000000000000 0000000000000000000000000 40 0000000000000000000000000 O0000000000000000000000000 50 21 JP 2015 528310 A 2015 9 28 39 40 El EJ EJ OOOO Ol EF EE ES rn 80 ven E ES E33 ES E
33. HPPD plas pln plo TAARIA ay 0000000000000000000000000 HPLC HPLC HPLC RR ER NE ee PfHPPD 49 4 7 47 P G336W 4 6 4 2 4 3 PIHPPDEvo3T 41 36 nd C023E6 4 3 3 5 n d EAE SER SEERE E ae lal m grad ect a 024 ei a pun DTE Op MAH Oa CC S PfHPPDEvo33 52 3 6 nd Li LuLL IZIEgeu o0 UUUU PfHPPDEvo36 52 42 n d 10 nnnnnn PfHPPDEvo40 50 32 nd CO210d10 5 9 3 9 nd CO212f3 48 38 n d 150 HGD 7 4 C644 5 5 4 5 5 1 HRAHB2012 028579 BAMB2012 02857 47 5 1 BBV CHESNAILAHRE 4 OBI CHES SILAS C645 52 c0218A5 5 0 3 7 46 253 F4 278 c0216C6 4 8 3 1 4 2 PfG336W 62 6 6 c0213H10 4 5 3 4 4 3 PfHPPD 6 4 5 9 n d PfHPPDEvo40 5 5 6 1 PfHPPDEvo41 49 34 022869 46 42 42 PfHPPDEvo4l 54 56 C0232D2 n d n d n d C0234A4 5 0 n d nd O Apmmnmmmnmn mn 5 s 2
34. ER cs C0240D12 4 9 n d n d 00000000 0000000 00000000 30 0000000000000000000000000 0 0000 s 0000000000000000000000000 000000 HPPD plso ploo Em Km E FRYT QM 558 40 HGD HGD HGD KOMMEN PfHPPD 6 4 5 8 5 8 187 1 LC UTE AY AOV 5477 EEG e P G336W 6 0 53 55 141 2 UUUUUU PfHPPDEvo33 6 2 46 5 5 581 6 8 PfHPPDEvo40 6 2 42 48 509 PfHPPDEvo41 5 6 42 43 336 16
35. 3 47 JP 2015 528310 A 2015 9 28 91 92 PfG336W Axmi309H Axmi428H Axmi305H PfG336W 100 94 56 53 Axmi309H 94 100 56 54 Axmi428H 56 56 100 54 Axmi305H 53 54 54 100 o 20 Y plso plso plso Km uM FuvBbVAY 72 baby 27 IFY PfG336W 6 4 5 8 5 8 187 30 PfHPPDEvo40 6 2 4 2 4 8 509 PfHPPDEvo41 5 6 42 43 336 Axmi305H 6 7 6 5 6 7 116 Axmi305H Evo40 5 8 55 5 3 721 Axmi305H Evo41 6 8 5 0 5 2 816 Axmi309H 6 4 6 4 6 1 318 Axmi309H Evo40 5 9 4 4 5 2 290 Axmi309H Evo41 6 2 45 4 7 807 Axmi428H 7 2 7 0 6 7 47 Axmi428H Evo40 6 1 5 2 5 5 1490 Axmi428H Evo41 5 6 43 5 gt gt 1000 40 50
36. 31 JP 2015 528310 A 2015 9 28 10 20 8 8 3 32 JP 2015 528310 A 2015 9 28 61 62 o 1 20 OO ES EE FEET E SENER E ED ES EE EIE ET
37. ESI E33 EI ENE 1 E E EJ EJ ES EJ EJ EJ E4SEdEJ EJ EJ EJ EI E EJ Ed EJ EJ EJ E EJ EJ 39 JP 2015 528310 A 2015 9 28 L3 C3 L3 L3 EJ ooo E EE d E EJ Ed ET E EET E3 E E a Ea io Ee Ea Es en 10 EJ EJ E EJ EJ Ed EJ LE 1 ET ESSE ER EST E ET EISE E L3 L3 L3 L3 20 SELE EE OO Eg E EpL T EE eS SE LT Er LIES EI ea ed eats fp Ys ea Peay Ral Ged Ee ed oo Ep AE eus EIE EE EE EI EISE E EIL E 1 1 EST TESTES ESSE TES L
38. 30 0000000000000000000000000 4 000 0000 0000000 0 000000 000 0000 0000000 0 000000 0000000000 000000 0000000 50 5 JP 2015 528310 A 2015 9 28 7 8
39. 4 JP 2015 528310 A 2015 9 28 84 HPPD plso plso Vr baby AY OD OD PfHPPD 25 6 25 6 PfG336W 49 5 3 PfHPPDEvo37 AT 5 3 C023E6 4 4 5 5 C024H11 4 1 4 6 PfHPPDEvo33 4 3 5 1 PfHPPDEvo36 4 5 E n d PfHPPDEvo40 3 5 49 CO210d10 43 5 7 0021289 AT 5 4 C644 5 3 25 6 C645 gt 5 6 gt 5 6 c0218A5 44 5 1 c0216C6 4 0 4 9 c0213H10 4 9 5 2 PfHPPDEvo41 3 4 4 7 C0228G9 5 4 5 7 C0232D2 5 1 5 5 C0234A4 49 gt 5 6 C0235F6 4 7 5 5 C0235E2 4 7 5 4 C0236H7 4 7 5 3 C0236F8 4 9 25 6 C0240D2 49 5 4 C0240D12 4 5 5 0 C0242D4 3 6 4 5 C0244A2 4 7 5 6 C0244F5 4 3 5 3 C0247B6 5 1 gt 5 6 C0247H7 4 4 5 2 C0252F11 2 7 4 6 C0255B12 4 0 5 0 C0255C1 3 6 4 7 C0255C3 4 8 5 5 C0255E6 3 7 4 4 C0255E10 4 8 25 6 C0256B1 5 0 25 6 6025611 4 9 5 5 C0256H4 3 6 4 8 C0257C5 3 8 4 6 C0260E11 4 7 5 4 C0260C6 4 6 5 3 C0262C4 4 8 5 3 C0262F11 5 0 5 5 C0263B7 4 1 4 6 C0263G12 4 9 5 6 C0261H2 4 5 5 3 C0264G5 4 9 5 6 C0264G7 4 1 48 C0266A11 4 0 49 0000 0000 0000 4 JP 2015 528310 A 2015 9 28 85 86
40. o ER pb ee Ek HE ES EF Ee NE TESTE EF Ea EF EST ECT E EST ER EF FE EST EST Seda ERI ESI S EST REST AEST LECT JE SE Oooo 8 Ez E E EF SES E E EZ E ot Uu 8 nooo foooooo Pt Cd MEK OOOO OO OC LJ E LJ et ae Er EFA OOOO OO OC OOOO OO OC iy Ed EN EVE 1 Ea Ez Ea EE EI ET eed 1 1 1 1 EI ECES EE EJ ET OC EN EET LL 6E Ee 3 EJ EJ EJ
41. 50 45 JP 2015 528310 A 2015 9 28 87 88 HPPD mmurmokK H srn DK tY b PfG336W 2248 811 160 7 12 Wigs PfHPPDEvo40 207 60 65 314 LI LI L1 LI LI LP LI LJ LI LI LJ LI LJ 2 LI LI LI LI LJ 03 LI LI LJ PfHPPDEvo41 852 412 279 32 75 PfHPPDEvo36 E 88 47 18 20 45 EE SENE EERE 10
42. E Sy ES 1 el L3 L3 L3 L3 OO EH EJ EJ EJ OOO E dE EE EI Ee E 1 EI Tz BE ED a 82 o 24 EEN EJ 1 EX E EJ E 50 E END S 40 JP 2015 528310 A 2015 9 28 L3 LJ EJ E Cr EH U D Li LI LI LI LI Li LI LI LI LI Li LI LI LI L2 LI LI LI LI LI LI L1 LI LI L3 LI LJ LI LI LI LI LI LI LI 11 LI LI 00000000000000 00000000 0000000000000000000000000 00000000 000000000000000000 000000 000000000000000000000000 000000000 000000000000 000000000000000000000000 0000000000000000000000000 00000 10 0000000000000000000000000 000000009 000000000000000 000000000000x00
43. 8 3 33 JP 2015 528310 A 2015 9 28
44. OOOO OO OC ET a EX I EVER OO OC EPE Lt EJ 1 4 OC EE EST EET 1 Et E Ez CES E E ET EST AEST EST EST ESTE E ESI ET 1 2 EE GE I 9 JP 2015 528310 A 2015 9 28 15 16 000000000000000000000000 10 0000000000000000000000000 000000000000000000000000 20 0000000000000000000000000 3 00 0 00 000 00 000000 0 0 000000000000000000000000 000000000000 000 0 000000 000000000000000000000000 000 0 0000000000 0 00 000 00 000000 00000000 0 000 00 000000 0 000000000000 00000000 0000000 0 000 0 0000000000000000000000000 00 0000000 0 00000000000 0000000000000000000000000 4 0000000000000000000000000 000000000000000000000000 50 0000000000000000000000000
45. AEST LTO ELT TE EF ES EV OE LEE TI EIE Be de Ea SESE LE sea ELEVEN EST ES TEST 2 ES EST EST AES TEST 2 E ELI ES EDI ERI EVEN EST FOER JE Xu HPPD 32 5 79 0 04 W336 152 35 2 49 NT Km HPP uM Vmax Ki TBT uM Axmi428H 52 22 6 1 37 A ET ELC BENENE EST 1 ET S ESI TELLE ELLEVE EE EI Ed EET E EA 1 CIF elie Ee SIS E ESL ke ES EST AEST 1151 L3 L3 oOo SEER Ee Ep Es ESI Coy EEN EIE EI LE DO EE ES EST 3 E L3 L3 EJ oOo oO E EE EIE TE ELT EVE EJ EJ EIE BS E EXCESS E CEFE ERTI EHE EF Ez 3
46. 49 59 95 95 100 74 99 94 98 94 76 BE 143 144 144 109 149 138 144 141 120 134 190 191 191 159 196 185 191 191 170 HOC myy ayy hytesy AVS RRA TAN Vad RFA AAA ayyy mA CREER 74 IPAR ES xaT AhRbWTZbv4 XASYAMRT4UA vu4xtXk AX zwyv 5 AYYIRAFR IFAR 3048 mur bh tnay 2 77 EAS TA SES 49A eee Sil 117 AA OC wur myy b tnay AUT 7 3 u4XTXY PAIR aAZUVUXATAUARTAR SOC xu 7 7 2 7 37 4 33 FAR APYYAAFRAART AA rwAT BYARD hoeoay ABRWZRY4 ASOYAMiT4VA FAL AGGERE 4317413 JP 2015 528310 A 2015 9 28 5 S1YDIDPVYLEG D LPFLPGFERV VERNPVGAGLEVIDHLTHNVYRGRMVYWANFY 179 SPGAVDYGLTRFKOHVVGN VPEMAPVIDYM 231 D LPPLPGFERV SPGAVDYGLIRFDHVVGN VPEMAPVIDYM 212 AG EPFLPGTEGV SPGAADYTGLSRFDHIVCH VPELAPAAAYF 233 G PYLPGYVAAAPIVEPPAHR TFQAIDHCVGNVELGRMNEWVGEY 203 TEESEFLPGFERVEDA 55FP LDYGIRRLDHAVGN VPELGPALTYV 241 D VPFLPGFEGVT HPDAVOTGLIRFDHVVGNH VPELAPAAAYI 226 GLFLPGFEAVEGI ASFPDLDYGIBRLDHAVGN VTELGPVVEYI 234 PFLPOTRSCITVOSAHKFLPPVHLEATDHCVGHQUWDEMSDACDFY 234 GFMPGYRMESHADATZKFLPEVVLERIDHCVGRQDWBEMERVCDYY 217 EKLEHFREARYP DIKGEYTGLTSKAMSAPDGMIRIPLHE ESSKGA 224 KGF
47. 8 8 3 12 JP 2015 528310 A 2015 9 28 DD 2 00000000 1000000000000000000000000 2 0000000000000000000000000 1000000000000000000000000 0000000000000000000000000 O000000000000000000000000 50 13 JP 2015 528310 A 2015 9 28
48. M ME M3 MK W W NA NG N NQ NZ CM PA PE PG PH PL PT QA RO RS RU RWSA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ 74000 100126099 74000 100188651 2000 00000000 2000 2000 000000000 723000 00000000 2000 O000000000 2000 000000000 OO00 00 28030 A05 CA17 48024 CA DOS FAA 4B050 4984 LL10 48064 1 CA19 DA12 48065 ABOl BAO2 CA28 253
49. Synechococcus D D Rhodococcus OOOOOOOO000000000 O00000000 000 0 B ephari 5 0000000000 PicrophilusyQO000000 abi dopsis Q OOOOOO000000 O0Q00000000000000000000000 30 0 0 Aena sat 000000000000000 Brachiaria pl at DU Cenchrus echi 000000000 Lol i umri gi dump 0000000 Festuca arund 4 ined OOOO0000000000000000 000000 Setaria faberi 9 0000000000 00000000000 E eusine indic 0 00000 tSoerehum D D
50. 0000x00 000000000000000000000000 O000x00 0000x00 0000x0 000000000000000000000000 070000x007 0000x0070000 00000000 000000000000000 000x007700000000000000000 000000900000000000000000 0000000000000000000000000 000000002 0000000000000 00000000000000000 00000 20 00 00 20 00 CIT praw gt 56 49 53 000000000000000000000000 00000000000000 n 30 000000000000000 ie Keat s ng 7 72 PfHPPD 187 4 4 0 76 uie S T E ON FURRY AY 77 PfHPPD 4 9 4 7 4 7 PfG336W 4 6 4 2 4 3 000000000000000000000000 000000 000000000000000000000000 0000000000000000000000000 000000009900000000000000 0000000000000000000000000 000 40 D 000 000 0000000000 0000 000000000000000000000000 0000 0000000000 000000000000000 000040 000000000000000000 00000 0000000000000000000
51. 19 a0 LI EI LI LI U 1 2015 528310 OO 5900 270 9 281 2015 9 28 15 09 2006 01 121 2006 01 5 10 2006 01 2 02 2006 01 100 2006 01 Oooo Ez Ez DUO oo Ed 2 ES 2 D 000000 N N M 2015 532063 P2015 532063 257 99 13 2013 9 13 0 0 270 50 120 2015 5 12 PCT US2013 059598 W22014 043435 28 30 201 2014 3 20 OO 617701 037 249 91 149 2012 9 14 Ub 61 766 057 0 0 250 20 189 2013 2 18 08 61790 404 250 30 150 2013 3 15 Ez eo yaana LJ 5 O1 Ex E 7 Oooo EE EJ Ee OOO 1 L 0000p D 0000000 D 000000000 0
52. E 1OL E ESL SE E E11 ET EEN EJUET E ee ELLE ECCLE EJ 111 1 2 EVE EE E E EZ E E E EJ ES EJ EYE ESI ERE ET Ged ES ESL SEE ECT EST E237 EI EST Sead Ea 77 DESI Ed Ee ES EET EE AEST ESL EE al IES eS SE SESSEL RA Cp SES Ed LEE EVE EFE EZ ESTIS DEEST CHEST EST ETE EE ETE EISE SEITE EJ EST EAE 59 5 E E EVE SEK EVE ENE NEED EVE ads ENEDES ENTEN EVE ME EET pm mE ELE EI ER sd et ey ae es ESI ERE 3 Et i ED ESKE G EST LESE EET Ed EST EST LET EIE ES EET EE ET a EST EST TEST E ESI ETE ET 4 1 Ea ESSE ESTE ESSE E SE EYES Ez SE SERIE E Ez EHE Ed EJ EN E EJ DEJES
53. OOOO Ol OOOOH L ET ET OOOO Ol EES 25 Ee Hd o N e 8 8 3 22 JP 2015 528310 A 2015 9 28 OQ00000000000000000000000 10 O000000000000000000000000 O000000000000000000000000 ao O000000000000000000000000 30
54. acid substitution for proline 56 JP 2015 528310 A 2015 9 28 International Application No PCT US2013 059598 FURTHER INFORMATION CONTINUED FROM PCTASA 210 serine histidine alanine glycine or glutamine at a position corresponding to position 335 of SEQ ID NO 1 Use of the polypeptide polynucleotide for the generation of transgenic plants tolerant to an HPPD inhibitor herbicide A commodity product comprising a detectable amount of the nucleic acid or the polypeptide A method of controlling weeds in a field comprising the transgenic plant 4 claims 1 34 partially A recombinant nucleic acid molecule encoding a 4 hydroxyphenylpyruvate dioxygenase HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and having at least an amino acid substitution for serine or tryptophan at a position corresponding to amino acid 336 of SEQ ID NO 1 A vector host cell transgenic plant or transgenic seed comprising said nucleic acid A recombinant polypeptide comprising an HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and comprising an amino acid substitution for serine or tryptophan at a position corresponding to position 336 of SEQ ID NO 1 Use of the polypeptide polynucleotide for the generation of transgenic plants tolerant to an HPPD inhibitor herbicide A commodity product comprising a detectable amount of the nucleic acid or the polypeptide A method of controlling weeds in a field comprising the
55. amino acid sequence s Continuation of Item 1 c of the first sheet With regard to any nucleotide and or amino acid sequence disclosed in the international application and necessary to the claimed invention the international search was carried out on the basis of a means on paper in electronic form in the international application as filed together with the intemational application in electronic form subsequently to this Authority for the purpose of search O In addition in the case that more than one version or copy of a sequence listing and or table relating thereto has been filed or fumished the required statements that the information in the subsequent or additional copies is identical to that in the application as filed or does not go beyond the application as filed as appropriate were furnished Additional comments Form PCT ISA 210 continuation of first sheet 1 July 2009 54 JP 2015 528310 A 2015 9 28 International application No INTERNATIONAL SEARCH REPORT PCT US2013 059598 BoxNo ll Observatlons where certaln clalms were found unsearchable Continuation of Item 2 of first sheet This international search report has not been established in respect of certain claims under Article 17 2 a for the following reasons Claims Nos because they relate to subject matter not requiredto be searched by this Authority namely 2 L Claims Nos because they relate to parts of the international appl
56. of controlling weeds in a field comprising the transgenic plant 58 JP 2015 528310 A 2015 9 28 INTERNATIONAL SEARCH REPORT Information on patent family members International application No ERE cited in search report date insani date EP 2453012 Al 16 05 2012 2453012 Al 16 05 2012 2669369 Al 04 12 2013 2669370 Al 04 12 2013 2669371 Al 04 12 2013 2669372 Al 04 12 2013 2669373 A1 04 12 2013 WO 2009144079 Al 03 12 2009 071316 A1 09 06 2010 C 2724670 Al 03 12 2009 101998993 A 30 03 2011 2268815 Al 05 01 2011 2011039706 A1 17 02 2011 2009144079 A1 03 12 2009 WO 2011053557 05 05 2011 078813 A1 07 12 2011 C 2774441 Al 05 05 2011 2493931 A1 05 09 2012 2011104755 A1 05 05 2011 2011053557 A1 05 05 2011 Form PCT ISA 210 patent family annex April 2005 59 JP 2015 528310 A 2015 9 28 5 Int d OOOO 904 2006 01 9 04 80 AP BWGH GM KE LR LS MJ MZ RWSD SL SZ TZ UG ZM ZW EA AM AZ BY KG KZ RU TJ T M EP AL AT EE BG CH CY CZ EE ES Fl FR R FR HJ I E IS LT LT LU LV M5 MS MF NL NO PL PT ROR S SE Sl SK SM TR ON BF BJ CF G d M CA QN GQ GWKM M M Ne SN TD TG AE AG AL AM AT AU AZ BA BB BG BH BN BR BW BY EZ CA CH CN Q CR CZ EK DM DO EC EE EG ES Fl GB G9 GE H GM GI FN H R HJIDIL INIS J P KE KG KP KR KZ LA LC LK LR LS LT LU LY
57. vector host cell transgenic plant or transgenic seed comprising said nucleic acid A recombinant polypeptide comprising an HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and comprising an amino acid substitution for glutamine alanine valine or glutamic acid at a position corresponding to position 340 of SEQ ID NO 1 Use of the polypeptide polynucleotide for the generation of transgenic plants tolerant to an HPPD inhibitor herbicide A commodity product comprising a detectable amount of the nucleic acid or the polypeptide A method of controlling weeds in a field comprising the transgenic plant 7 claims 1 3 6 17 22 34 all partially A recombinant nucleic acid molecule encoding a 4 hydroxyphenylpyruvate dioxygenase HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and having at least an amino acid substitution for leucine at a position corresponding to amino acid 215 of SEQ ID NO 1 A vector host cell transgenic plant or transgenic seed comprising said nucleic acid A recombinant polypeptide comprising an HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and comprising an amino acid substitution for leucine at a position corresponding to position 215 of SEQ ID NO 1 Use of the polypeptide polynucleotide for the generation of transgenic plants tolerant to an HPPD inhibitor herbicide A commodity product comprising a detectable amount of the nucleic acid or the polypeptide A method
58. 00000 0000000000000000000000000 0000000000000000000000000 000000000000000000000000 8 0 000000007 00000 000000000 000 00000 5 0000000000000000000000000 41 JP 2015 528310 A 2015 9 28 79 80
59. DLE AEST 1 EST EZ EZ ENE EVERETT 1 E T EzJ ESI ed em LIEST Ez EZ ET EE EE AEST EI LJ EST EVEN FIE LES ESI ES EST AEST SES EST AEST AEST an H A GAGO E GA ERr eaa AG GA G G E 3 20 JP 2015 528310 A 2015 9 28 0000000000000000000000000 19 00000000000000 000 0 saturation m tagenesi s 000 0000 pernatati onal nut agen
60. ESFALGAPLAARSDLSTGNSAHASLLLRSGALAFLE VELWCADAASAAGRESEGLGAPLAARSDLSTGHSAHASLLLRSGSLSELF VVFAVGHAKQAA HYSTAFGMOLVAYSGPEHGSRETASYVLTNGSARFVL IEFWCGDATHVARRFSWGLGMRFSAKSDLSTGHHVHASYLLTSGDLRELF VEFWCADAASAAGRFAFALGAPLAARSDLSTGNSAHASQLLRSGSLAFLF IEFWCGDATHTSRRESWGLGMPLVAKSDLSTGNSVHASYLVRGANLSEVE AEWHWVGNAKQVAQFY ITRHMGFEPVAHKGLETGSREEASHVVQRNGVREVE YVHWYVGNAKQAATYYVTPMGFERVAYRGLETGSKAVASHVVRIGHITEIL WHEPHER IASYFAAEHGPSVCGHAFREVEDS TAPYAPPPQEA AT AATASIPZFSADAARTFAAAHGLAVRSVGVRVADA TIAPYAPPPQEA ATAAATASIPSPSADAARTFAAAHGLAVRSVGVRVADA IAPYAH GADAATAALPSFSAAAAPRFAADHGLAVRAVALRVADA TSVIKPATPUG HFLA DHVAERGDGVVDLAIEVPDA TAPYZPSLSAGEIKPITIASIPSFDHGZCRZFFSZHGLGVRAVAIEVEDA TAPYAH GUDAATASLPSFSADAABEFZADHGIAVRSVALRVADA TAPYZPSTTT SSGSAAIPSFSASGEHZFAAKHGLAVRAIALEVADV TSPVRSSARQT LEAAPLADQARL EMYDHLDKRGDGVEDVAFEVDDV TSPLRSVEQAS RPPE DEALLKEIHABHLERHGDGVEKDVAF EVDCV B ot QKAYNRALELGAQP IHIDTGPMELNLPAIKGIGGAPLYLIDRFGEG AEAFRVSVAGGARPAFAPADLG HGFGLAEVELYGDVVLRFVSGYPDET AEAFRVZVAGGARPAFAPADLG HGFGLAEVELYGDVVLREVSYPUET EDAFRASVAAGARPAFGPVDL G RGFRLAEVELYGDVVLRYVSYPUGA RAAHAYAIEHGARSVAEPYELKDEHGTVVLAAIATYGETRHTLVDURTOYD ESAFZISVAHGAIPSSPPIVLH EAVTIAEVELYGDVVURYVSYKAED AEAFRASBRRGARPAPAPVDLG RGFAPAEVELYGDVVLREVSHEDGT AAAFEASVARGARPASAPVELD DQAWLAEVELYGDVVLREVSFGREE LAVTEHAVAHGAESVSSPHTDSCDEGUVISAAIETYGDTTHTEIQRTTYT ESVESAAVRHGAEVVSDVRTVEDEDGQIKMATIRTYGETTHTLIERSGYR
61. IRLKROGLVLOEDFETLKSLDILI RDDOGILLOIFTEPL 385 RDDOGVLLQOIFTKPV 370 RDDOGTLLQIFTKPV 378 FDEGGYLLQLFTEPL 378 FDEBGYLLOLFTKHL 357 MG PVFFEFIQRK GDDGFGEGNFEALFESIERDQ 343 GDRPIFFLEMIQRIGCMEKDEVGQEYOKGGCGGFGNGHEFSELERKOIEDYE 425 GDRPIFFLEMIQRIGCHEKDEVGOEYOKGGCGGFGEGHEFSELFKSIEDYE 426 GDRETLFLELIQRIGCMEEDERGUEYOQKGGCUGFGNGUFSQLFKZIEDYE 427 QDRPTVEPETIERH GSHGFGEGNFEALFEAIEREQ 374 GDRPTIFIELIQRVGCHMMEDEEGKATQSGGCOGFGEGNFSRLFKSIERYE 435 GDRPTLELEHIQRIGCHERDERGEEYQKGGCGGEGKGNFSELFESIEDYE 420 GDRPTLFIEIIQRVGCHMLKDDAGQMYOKGHCOGFGEGNESELEKSIEEYE 428 MORPTVFIELIQRN WFDGFGAGHFESLFEAIEREQ 413 MORPTVEIELIQRN HFSGFGAGNFRALFEAIEREQ 392 VRRGVLTAD ESLEVKQSVVAQKS KSLEVKQSVVAQKS KSLEAKQAARAAAAQGS 444 50 JP 2015 528310 A 2015 9 28 2015528310000001 app 51 JP 2015 528310 A 2015 9 28 INTERNATIONAL SEARCH REPORT PCT US2013 059598 A CLASSIFICATION OF SUBJECT MATTER INV C12N9 02 C12N15 82 ADD Anoording to Intemational Patent Classification IPC or to both national classification and IPC B FIELDS SEARCHED Minimum documentation searched classification system followed by classification symbola C12N Documentation searched other than minimum documentation to the extent that such documents are included in the fielde searched Electronic data base conaulted during the intemational search name of data base and where practicable searc
62. JP 2015 528310 A 2015 9 28 8 10 0 0000 20 0 0o00 0 00 000000 DU 40 0o00 0o00 0o00 O00 0 0 0 D OO 000000 0 D 0 0 0 DU 0 0 0 O00 0 0 0 00 00000000 O00 0 50 D 0 0 00 0000 00000 29 JP 2015 528310 A 2015 9 28 nHonnnnnnnnnnnnonnnnns noone 10 N e 8
63. LGFHEFAEFTAEDVGTITESGLHSVVLAMHSEAVLLPLNEPVHGTKRR 281 KGFLGFHEFAEFTAEDVGTITESGLNSVVLANNGEAVLLPLNEPVHGTKRR 282 AGFTGFHEFAEFTTEDVGTAESGLESMVLAMNGENVLLPLNEPVHGTKRR 283 HEVMGETHMKEFVGDDIAIEYSALMSEVVADGILKVEFFINEFALAKE K 252 AGFTGFHOFAEFTADUVGTAESGLEGAVLASNDEMVLLPIMEPVHGTKRK 291 AGFTGFHEFAEFTAEDVGTTESGLHSVVLAMHSEGVLLPLNEPVHGTERR 276 FGPTGFHEFAEFTARDVGILEZSGLNSVVLANHMEEMVLLPLHEPVYGTERK 284 ERCLGFHRFUSVDDEDICTEFSALESIVMSEPNQUVEMPINEPAHGEK K 287 EKILGFHRFHSVDDEDICTEFSALESIVMASPHDIVEMPINEPAKGKK Q 286 jat 423 Mut GOIEEFLMQFHGEGIQHVAFLIDDLVETRDALKKIG MREMTAPPDT 270 SQIQTYILETHGGPGVONIALASHDVLRTILREMRARTPMGGFEFMAPPQAK 331 SOIQOTYLETHGGPGVOHIALASNDVLRTLREMRARTPMGGFEFMAPPQAK 332 SQIQTFLOHHGGPGYQHMALAHDDVLRTLREMQARSAMGGFEFMAPPTZD 333 SQIDETLEFYGGAGVQHIALNTGDIVETVRIMRAA GVQFLDTP DS 297 SQIQTYLEHMEGAGLOHLALHSEDIPRTLREMBKRSSIGGEDEMPSPEPT 341 BSOIQTPLEHHGGPGVOHIAVASSDVLRTLREMRARZAMGSGEDELPPPLPK 325 SOIQTYLERNEGAGVOHLALVSEDIFRTLREMBKRSCLGGFEFMPSPPPT 334 SQIEEYVDFTNGPGVOHIALRTPHIIEAVSHLRSR GVEFISVP DT 332 SOIKEYVUFYNGAGVQHIALRTNHIIDAITHLEAR GTEFIKVP ET 311 YYEMLEGRLEDHG EPVDQLQARGILLDGSSVEGDKRLLLOIFSETL 316 YYEGVRRIAGOVLS RDUQUVLLOIFTKPV 375 YYEGVRRIAGDVLS RDDQGVLLOIFTKPV 376 YYDOGVRRRAGDVLT EAQLKECQE LGVLVD RDDQGVLLOIFTEPV 377 YYDILGEWVODT RVPVOTLRELKILAD RDEDGYLLQIPTKPV 339 YYQNLEKRVGDVLS DDOIKECEELGILVD YYEGVRRLAGDVLS EAQIKECQELGVLVD YYKHLENRVGDVLS DEQIKECEDLGILVD TYENMRLRLEAAGMELEESFDIIQKLIILID YYEDMK
64. ST FE ELT E E31 Ez EJ LE ooo E EE Ez LI EIE Fis I OO 20 EJ LJ EJ EJ E E I o O ER EI EMEF EET E ES E EVEN EST SERT d OOOO OOOO EN Ea EI E T E EF IE E Es Ee er EF Ep 1 qup le 3 Ho 00 EE LES EE EET EX I SET Ea EJ EI E ooo ooo ooo ooo E EIE ooo Bie El EE Ed ooo ooo ooo ooo ooo oo ooo EJ E ed Ed st St I LJ Cd a EI E EIGEIOEY EE OoOOOFOFOOOOOOOL ad ESI Ea Esa E 11 eat E ET Ee ESTE oEg EX EJ EX EE EZ 1
65. e protest fee was not paid within the time limit specified in the invitation O No protest accompanied the payment of additional search fees Form 210 continuation of first sheet 2 April 2005 55 JP 2015 528310 A 2015 9 28 International Application No PCT US2013 059598 FURTHER INFORMATION CONTINUED FROM PCT ISA 210 This International Searching Authority found multiple groups of inventions in this international application as follows 1 claims 1 34 partially A recombinant nucleic acid molecule encoding a 4 hydroxyphenylpyruvate dioxygenase HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and having at least an amino acid substitution for tryptophan glycine or serine at a position corresponding to amino acid 188 of SEQ ID NO 1 A vector host cell transgenic plant or transgenic seed comprising said nucleic acid A recombinant polypeptide comprising an HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and comprising an amino acid substitution for tryptophan glycine or serine at position corresponding to position 188 of SEQ ID NO 1 Use of the polypeptide polynucleotide for the generation of transgenic plants tolerant to an HPPD inhibitor herbicide A commodity product comprising a detectable amount of the nucleic acid or the polypeptide A method of controlling weeds in a field comprising the transgenic plant 2 claims 1 3 6 17 22 34 all partially A r
66. ecombinant nucleic acid molecule encoding a 4 hydroxyphenylpyruvate dioxygenase HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and having at least an amino acid substitution for serine cysteine or arginine at a position corresponding to amino acid 189 of SEQ ID NO 1 A vector host cell transgenic plant or transgenic seed comprising said nucleic acid A recombinant polypeptide comprising an HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and comprising an amino acid substitution for serine cysteine or arginine at a position corresponding to position 189 of SEQ ID NO 1 Use of the polypeptide polynucleotide for the generation of transgenic plants tolerant to an HPPD inhibitor herbicide A commodity product comprising a detectable amount of the nucleic acid or the polypeptide A method of controlling weeds in a field comprising the transgenic plant 3 claims 1 34 partially A recombinant nucleic acid molecule encoding a 4 hydroxyphenylpyruvate dioxygenase HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and having at least an amino acid substitution for proline serine histidine alanine glycine or glutamine at a position corresponding to amino acid 335 of SEQ ID NO 1 A vector host cell transgenic plant or transgenic seed comprising said nucleic acid A recombinant polypeptide comprising an HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and comprising an amino
67. h terms used EPO Internal BIOSIS EMBASE WPI Data C DOCUMENTS CONSIDERED TO BE RELEVANT Citation of document with indication where appropriate of the relevant passages Relevant to claim No X EP 2 453 012 A1 BAYER CROPSCIENCE AG 1 34 DE 16 May 2012 2012 05 16 abstract paragraph 0055 paragraph 0066 paragraph 0072 paragraph 0073 paragraph 0083 paragraph 0089 paragraph 0090 paragraph 0091 WO 2009 144079 A1 BAYER BIOSCIENCE NV BE BAYER CROPSCIENCE AG DE BUSCH MARCO DE 3 December 2009 2009 12 03 abstract page 5 line 8 line 33 page 13 line 9 line 30 Further documents are listed in the oontinuation of Box C See patent family annex Special categories of cited documents T later dooument published after the international filing date or priority date and not in conflict with the applioation but cited to understand A document defining the general state of the art whioh is not considered the principle or theory underlying the invention be of particular relevance E earlier application or patent but published on or after the international X document of particular relevance the claimed invention cannot be filing dale considered novel or cannot be considered to involve an inventive document which may throw doubts on priority olaim s or whioh is step when the dooument is taken alone cited to establish the publication date of another citation or other Y document of pa
68. ication that do not comply with the prescribed requirements to such an extent that no meaningful international search can be carried out specifically Claims Nos because they are dependent claims and are not drafted in accordance with the second and third sentences of Rule 6 4 a Box No Il Observations where unity of Invention Is lacking Continuation of item 3 of first sheet This International Searching Authority found multiple inventions in this inlemational application as follows see additional sheet s all required additional search fees were timely paid by the applicant this international search report covers all searchable claims s all searchable claims could be searched without effort justifying an additional fees this Authority did not invite payment of additional feea 3 s only some of the required additional search fees were timely paid by the applicant this international search report covers only these claims for which fees were paid specifically claims Nos No required additional search fees were timely paid by the applicant Consequently this international search report is restricted to the invention first mentioned in the claims it is covered by claims Nos 1 34 partially Remark on Protest The additional search fees were accompanied by the applicant s protest and where applicable the payment of a protest fee The additional search fees were accompanied by the applicant s protest but the applicabl
69. iumm OOOO00 u 0000 tica 0 D UU D U D oag OOO 0000 x enthumog 000000 30 0000 0000 0000000 OOOO O 00000 0000 0 000000000000000000 4 O0000000000000000000000000 50 000000 37 JP 2015 528310 A 2015 9 28 71 72
70. jw jv je CO212f3 0 42 1 C644 0 38 11 C645 0 36 1 c0218A5 0 13 13 c0216C6 0 50 E EE EE A AL 14 c0213H10 051 15 EU a Eel E E E E A OOOO E EI ESL 294 LE LE PfHPPDEvo41 0 44 16 C0228G9 0 17 17 40 C0232D2 is E E 033 19 C0235F6 0 53 02352 21 C0236H7 0 36 C0236F8 0 47 23 roo C0240D2 0 32 m C0240D12 0 29 25 C0242D4 0 21 26 C0244A2 0 25 27 C0244F5 0 22 EB CO247B6 0 37 29 83 C0247H7 0 28 B C0252F11 0 26 31 C0255B12 0 21 32 C0255C1 0 17 33 C0255C3 0 19 34 C0255E6 0 15 35 C0255E10 0 24 36 C0256B1 0 31 37 C0256G11 0 33 38 C0256H4 0 19 39 C0257C5 0 3 40 C0260E11 0 79 Al C0260C6 0 51 42 0026204 0 81 43 C0262F11 0 76 M C0263B7 0 43 45 C0263G12 0 77 46 C0261H2 0 42 47 C0264G5 0 71 15 C0264G7 0 35 49 C0266A11 0 33 56 a
71. llia D D a OD Sagi ttaria 0 OO Sci rpus D OO 000 Seta 50 rial 0000 Sorghumg 00000 0o00 pera OO D a Avena O00 0000 Brachia U chrus 0 0 0 0 0 0 Gome i na OO 00000 Gyno don 0 0 dd D Sypeeru n d B D D D D Da ctyl octeni ub tari a 0 Echi n 4 ochoa O00 D E eocharis Q OO 00 B eusi ne Eragrostis OO D Eri ochl o Festuca g Fi nbri styl isO OOO000 00 tcHteranthera 00 npe rata 1 0 0 D D schaenumo 0 0 0 Leptoch 36 JP 2015 528310 A 2015 9 28 69 70 0000000000000000 Awilod000
72. rticular relevance the claimed invention cannot be special reason ae specified conaidered to involve an inventive atep when the document ia dooument referring to an oral disolosure use exhibition or other oombined with one or more other such documents such combination being obvious to a person skilled in the art P document published prior to the intemational filing date but later than the priority date olaimed amp dooument member of the same patent family Date of the actual completion of the intemational search Date of mailing of the intemational search report 21 November 2013 11 02 2014 Name and mailing address of the ISA Authorized officer European Patent Offioe P B 5818 Patentlaan 2 NL 2280 HV Rijewijk Tel 431 70 340 2040 2 Fax 481 70 340 3016 Mundel Christophe Form PCT ISA 210 second aheet April 2005 page 1 of 2 52 JP 2015 528310 A 2015 9 28 INTERNATIONAL SEARCH REPORT International application No PCT US2013 059598 Category Citation of document with indication where appropriate of the relevant passages Relevant to olaim No WO 2011 053557 A1 MS TECHNOLOGIES LLC US SEKAR VAITHILINGAM US HELD BRUCE US CH 5 May 2011 2011 05 05 the whole document Form PCT ISA 210 continuation of aecond aheet April 2005 page 2 of 2 53 JP 2015 528310 A 2015 9 28 International application No INTERNATIONAL SEARCH REPORT PCT US2013 059598 Box No I Nucleotide and or
73. sis DO00000 li nsoga 0 OOO O00 Gli umm 0 D D H bi scus 0000000 Ipornoea 0 0 0 0 00 Kochia D D anum OOOO 000000 Lepidi um 0 Linderni a0 0 Mtri cari a Mentha n a gau Mercuri alis 0 0000 000 0 0 Milugo O00 000d Woseti s D D D Pa paver 00 00 D Pharbitis 0 OO 0 Plantago JOO Pertuaang 20 000000 O00 00 Ranuncul us 0 0 0 0 C Raphanus D OOO 0 Rorippa OOOO0 0 Rotala Q OO00 Rune 0 0 0 0 00 HI CSal sol Senecio 0 000000 0 0 Sesbani a 0 00000 Si dan 000000 S napi SD D DD Solanum D D D D 5 0G Sphenocl ea O St OOOO 0 Taraxacum 0000000 D pnugpnupnugg pauggupnaugd 00 D Thlasp 0 0000000 trifo
74. transgenic plant 5 claims 1 4 6 20 22 34 all partially A recombinant nucleic acid molecule encoding a 4 hydroxyphenylpyruvate dioxygenase HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and having at least an amino acid substitution for threonine alanine or serine at a position corresponding to amino acid 339 of SEQ ID NO 1 A vector host cell transgenic plant or transgenic seed comprising said nucleic acid A recombinant polypeptide comprising an HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and comprising an amino acid substitution for threonine alanine or serine at a position corresponding to position 339 of SEQ ID NO 1 Use of the polypeptide polynucleotide for the generation of transgenic plants tolerant to an HPPD inhibitor herbicide A commodity product comprising a detectable amount of the nucleic acid or the polypeptide A method of controlling weeds in a field comprising the transgenic plant 6 claims 1 4 6 20 22 34 all partially A recombinant nucleic acid molecule encoding a 4 hydroxyphenylpyruvate dioxygenase HPPD enzyme having at least 55 sequence identity to SEQ ID NO 1 and having at least an amino acid substitution for glutamine alanine 57 JP 2015 528310 A 2015 9 28 Intemational Application No PCT US2013 059598 FURTHER INFORMATION CONTINUED FROM PCT ISA 210 valine or glutamic acid at a position corresponding to amino acid 340 of SEQ ID NO 1 A
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