EpiScope® Promoter qPCR Array (Human)
Contents
1. http www takara bio co jp 5 5301 IV Thermal Cycler Dice Real Time System VI Appendix 10 1 EpiScope Promoter qPCR Array 20 qPCR Array EpiScope Promoter qPCR Array 4 2 PCR 22. TakaRa Tel 077 543 6116 Fax 077 543 197 http wWW taKara DiO CO jp 201203Da
3. http wwwr takara bio coJp 7 5301 3 PCR PCR PCR PCR 1 PCR 2 PCR 1 3 PCR 95 30 2 Step PCR 95 5 60 30 40 4 PCR A A Ct EpiScope Promoter qPCR Array Human AA t
4. 96 2 1 3 Input Bound 1 3 Unbound Bound EpiScope Promoter qPCR Array Human URL http www takara bio coJp qpcr array_tool HeLa S3 A549 DNA 1 5 ug EpiXplore Methylated DNA Enrichment Kit DNA 45 FT EL 1 1 ul PCR EbiScope Promoter qPCR Array Human SYBR Premix Ex 78g GC Perfect Real Time PCR 1 http wwwr takara bio coJp 9 5301 HeLa S3 A549 0 20 40 60 80 100 0 20 40 60 80 100 FT Em EL FT mm EL RB SFRP VHL WT LINE GAPDH 1 2 DNA
5. 5 EpiScope Promoter qPCR Array Human 1 2005 3 4 PCR 120 126 2 2008 PCR 3 1 RT PCR 39 43 CN 2 SYBR Molecular Probe Inc RA RR
6. 1 PCR 2 1 DNA SYBR Premix Ex Tag GC Perfect Real Time PCR 1 DNA PCR 1 40 SYBR Premix Ex Tag GC 2 Xx 12 5 ul 500 wl DNA 1 ul 40 ul dH20 9 5 ul 380 wl Total 23 ul 920 ul DNA DNA 1 20 100 ng 2 PCR 23 ul 8 1 9 207 ul 2 8 PCR 23 ul
7. A A 1 PCR Ct A 2 Ct Qty 2 CO A 3 gt Qty Qtby x 100 9 5E 07 http www takara bio co jp 8 5301 V B B 1 PCR Ct B 2 Ct Qty 2 B 3 Oty Ai 5 9 Ob Qpb 09 Qty 100 Qty Qty EpiScope Promoter qPCR Array Analysis Tool Ct
8. EpiXplore FT EL DNA A549 CDKN2A CDKN2B VI Appendix PCR gt IV ABI PRISM 7000 Applied Biosystems 7500 RealTime PCR System Life Technologies 1 DNA PCR 1 40 SYBR Premix Ex Tag GC 2 Xx 25 ul 1 000 wl ROX Reference Dye or Dye ll 50 x 1 1 ul 40 wl DNA 2 ul 2 80 wl dH20 18 ul 2 720 ul Total 46 ul 1 840 wl 1 Applied Biosystems 7500 Real Time PCR System ROX Reference Dyell ABI PRISM
9. PCR 1 PCR 2 3 PCR 10 DNA SYBR Premix Ex Tag GC Perfect Real Time PCR PCR 5 8 EpiScope Promoter qPCR Array PCR EpiScope Promoter qPCR Array Human PCR Y http www takara bio coJp 2 5301 PCR 1 2 PCR PCR J 9 8 4 2 1 9 09 0 2 4 8 10 123 14 1 18 20 822 2M 26 326 30 32 HH MH 38 40 Cydes 1 50 ul X30 x 3 50 lx 2
10. 5301 IfdH4 EpiScope Promoter qPCR Array Human vV201203Da EpiScope Promoter qPCR Array Human EpiXplore Methylated DNA Enrichment Kit 631963 631962 DNA DNA DNA PCR qPCR 25 30 LINE 1 DNA GAPDH SYBR Premix Ex 7ag GC Perfect Real Time RR071A B
11. OOOOOOOOO OO OO OO OOOOOOOO DOOOOOOO OO X Replicate 1 1 2 1 2 3 PCR Sealing Film Plate Sealing Pad Sealing Film PCR Sealing Film PCR PCR 4 24 4 qPCR Array qPCR Array
12. PCR 18 4 ul 3 2 EpiScope Promoter qPCR Array 1 6 ul 4 PCR 95 30 2 Step PCR 95 10 60 30 40 http wwwr takara bio coJp 11 5301 VII VIII SYBR Premix Ex Tag GC Perfect Real Time RR071A B Thermal Cycler Dice Real Time System TP900 TP960 Thermal Cycler Dice Real Time System Single TP850 TP870 96well Hi Plate for Real Time NJ400 Sealing Film for Real Time NJ500 NucleoSpin Tissue 740952 10 50 250 EpiXplore Methylated DNA EnrichmentKit 631963 631962 EpiScope Methylated HCT116 gDNA 3522 EpiScope Unmethylated HCT116 DKO gDNA 3521 EpiScope Promoter qPCR Array Human Primer Set ID
13. PCR SYBR Premix Ex 7gg GC Perfect Real Time RR071A B lt DNA gt DNA NucleoSpin Tissue 740952 10 50 250 DNA EpiXplore Methylated DNA Enrichment Kit 631963 631962 lll 20C 1 1 4 EpiScope Promoter qPCR Array 20 qPCR Array EpiScope Promoter qPCR Array 4
14. 7000 ROX Reference Dye 2 DNA DNA 1 20 100 ng http wwwr takara bio coJp 10 5301 2 PCR 46 ul 3 2 EpiScope Promoter qPCR Array 4 ul 4 PCR 95 30 2 Step PCR 95 C 5 60C 31 or34 3 40 3 7000 31 7500 34 Applied Biosystems 7500 Fast RealTime PCR System Life Technologies 1 DNA PCR 1 40 SYBR Premix Ex Tag GC 2 X 10 ul 400 ul ROX Reference Dyell 50 Xx 04 wl 16 ul DNA 08 ul 32 wl dH2O 7 2 ul 288 wl Total 18 4 wl 736 ul DNA DNA 1 20 100 ng 2
15. OOOOSSS S 1 1 2 http www takara bio co jp 6 5301 2 2 PCR EpiScope Promoter qPCR Array 1 qPCR Array qPCR Array IV 1 2 qPCR Array PCR 2 uu EpiScope Promoter qPCR Array Human PCR
16. ie nooo Fmt 2059 MOOO25 3 ceGz umm no mm 92 Neo 0 m eeers csm 2950 mo gm 9 eeot9 IO 4015 NOO237 im cPG um 9 nn Mewr 4555 Mo242 im 22 GO010022 PTGS2 5743 NM_000963 95 4 Heeg24 msi inee Mwoe2 5 em mAssn me Maio2 1 Hewee7 mm 55 m0 iw 29 L 1 GO10028 SsFRP1 6422 NM_003012 29 HG010029 7428 NM_000551 93 CpG 5 UTR Hr laeesn um 1 1 1 ml CpG CpG 500 bp http wwwr takara bio coJp 4 5301 PCR Thermal Cycler Dice Real Time System TP900 TP960 PCR S
17. x 3 Forward amp Reverse primer mix 2 5 uM 25 ul 1 2 ul lt gt El5lBlalelslslzl2 5 2 LelMI2llelMl2 16 el2 3 7 sl23lml ulslelztlzlsliel2 1219lel2el3z EE i EE EE Replicate 1 Replicate 2 Replicate 3 http www takara bio co jp 3 5301 gt No Primer SetID Symbol GenelD RefSeq neooi Mc 324 WM 001127510 157 cpG 3 na Mec 24 moo ie GPG js eneo4 mee 2 Mwo77 io nas ep 3705 Mooo5es7 0 1 s ns cewwi oo Need gw 7 meeo7 cwi seo WMoommee mees cez 4 mo 7 1 no om 9 Mwooaeo im Lo ero coewA io5 mn 3 0 Cp 1 na mB io Mooese 8 no gw 3 WMooneis4 5 1 no pei ie2 Mwooesm im ns peg 2 mo im
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