高効率リアルタイムPCR用マスターミックス「KOD SYBR® qPCR Mix」を
Contents
1. PCR illumina 1 RNA seq A D total RNA TruSed Standard mRNA illumina DNA PhiX Control Kit v3 illumina 375bp 1 RNA sed Primer 1 AAT GAT ACG GCG ACC ACC GAG AT 23bp Primer 2 CAA GCA GAA GAC GGC ATA CGA 1DDp PCRH DW 92 95C 10 min KOD SYBR qPCR Mix 104 1Opmol ul Primer 1 0 4 95C 10 sec 60C 30 sec x40cycles 10pmol ul Primer 2 0 44 50XRHOX reference dye 0 04 Melting DNA YA Data collectlon total 0 DNA 20pM 0 196Tween 0 Applied Biosyst2. PCR KOD SYBR qPCR Mix NGS PCR 200Dbp PCHR KOD SYBR qPCR Mix KOD DNA polymerase KOD SYBR Green I PCR 3 5 KOD exo DNA polymerase KOD 809 GC kb
3. BR qPCR Mix 50xROX reference dye 1 67mlx3 200 QKD 20 1 000 1 67mIlX3 5 1 000 QKD 20 1X 5 147 000 50xROX reference dye 50I KOD SYBR qPCR Mix SYBR Applied Biosystems Life Technologies Corporation illumina MiSeq TruSeq illumina Inc
4. C mm i FEELmP_ mn s et 4 1 RNA seq DNA KOD SYBR qPCR Mix A PCHR PCH 200bp 860bp Taq PCR 4 illumina illumina MiSeq A B Read 1 Read 1 a Dencity Cluster PF Phas Prephas Reads el ES Dencity ClusterPF Phas Prephas Reads mm2 M K mm2 M 1 38 1059 19 95 13 0 43 0 182 0 078 26 07 1 38 1157 33 93 89 0 73 0 161 0 092 28 32 Read 2 Read 2
5. Lane Ti Dencity Cluster PF Phas Prephas Reads Lanel Tl Dencity ClusterPF Phas Prephas Reads 0 M WW2 M 1 38 1059 19 95 13 0 43 0 186 0 073 26 07 1 38 1157 33 93 89 0 73 0 154 0 071 28 32 KOD SYBR qPCR Mix A B NGS Density Cluster PF 800 1300k mm 8096 KOD SYBR qPCR Mix NGS KOD SYBR qPCR Mix GC KOD SYBR qPCR Mix KOD SYBR qPCR Mix 1mlX1 40 QKD 201T 9 800 KOD SY
6. ems 7500 Fast MuItilNA DNA RNA DNA 4pM dPCR PCH PCR PCH ROX 1 DNA A PCR Taq KOD SYBR qPCR Mix B NGS 4 20pM ly 20pM 20pM 03125py 03125pM 033125pM li i li 901JKe7 2014 102 RNA seq 4pM RNA seq A G60Dp A PCH Taq KOD SYBR qPCR Mix B NGS imi tr gmhmnm FT i d
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