pCold™ Vector Set - Clontech Laboratories, Inc.
Contents
1. v201110Da 077 543 6116 TaKaRa 077 543 19772. TEE His Factor Xa pcoldIDNA O O O pceldIDNA O O pcoldIIDNA O pcoldVDNA 1 NewJersey 2 3 1 New Jersey Code 3360 3361 3362 3363 3364 3365 3371 2 New Jersey ProteinS Code 3371 4
3. pCold IV 4 pCold 1 TEE His Factor Xa pCold ll TEE His pCold ll tk TEE pCold IV multi cloning site ge site pCold O KIR 10mM Tris HCl pH8 0 1mM EDTA OR pCold I DNA 4 407 bp pCold II DNA 4 392 bp pCold III DNA 4 377 bp pCold IV DNA 4 359 bp 1 dideoxy 2 IRES Ndel Sach Koni Xholi BamHI EcoRI Hindili Sall Pst Xbal 1 cyp4
4. from this restriction controlled In addition 5 untranslated region 5 UTR translation 3 PURCHASER shall not utilize any partial sequences from PRODUCTS for the purpose enhancing element TEE His Tag sequence Facter Xa cleavage site of new plasmid construction using the Cold Shock Expression System 4 PURCHASER shall not transfer or sell copies of PRODUCTS components of ang multi cloning site MCS aE iocated atthe downstream ofthe CPA PRODUCTS derivatives of PRODUCTS and or products obtained through the use promoter As this product utilizes the promoter derived from coli most of PRODUCTS collectively DERIVATIVES to any third parties Notwithstanding E coli strains can be utilized as an expression host oregoing PURCHASER may transfer DERIVATIVES solely to a third party which has There are four kinds of pCold vectors l IV whose arrangements vary in already been granted a license to use PRODUCTS for research purpose through the existence of TEE His Tag sequence and Factor Xa cleavage site purchase of PRODUCTS from TAKARA BIO its subsidiaries or its local distributors provided that PURCHASER shall enter into a prior separate agreement with TAKARA Cold contains TEE His Tag sequence and Factor Xa cleavage site P gseq 3 BIO for the transfer of DERIVATIVES to said third party pCold ll contains TEE and His Tag sequence pCold Ill contains TEE only pCold IV contains no additional sequence at the u
5. Cold Shock expression vector pCold Vector Set Code No 3360 Size 5 u geach x 4 tubes Shipping at 20 C Store at 20 C 2 years from date of receipt under proper storage conditions Lot No Applications This is a vector for protein expression utilizing the promoter of cold shock Concentration amp Volume ee 1 pColdIDNA conc 9 ul vol Product list H 2 pCold II DNA conc ug l vol ul LpcoldiIDNA O O O 3 pCold III DNA conc ug ul pceldIDNA O O vol ul pColamoNna SO J 4 pCold IV DNA conc pg ul L pcoldIVDNA vol yl Regarding protocol please refer to the product manual supplied with this product 1 PRODUCTS are for research or laboratory use only PURCHASER understands and Description agrees that PRODUCTS shall not be administered to humans or animals and shall Cold shock expression vectors pCold DNA are designed to perform not be used for pharmaceutical in vitro diagnostic or any commercial purposes ffici d i ilizi tar derived A other than internal research emcient protein Expression ut iziniga Promoter Cer ved TOM 2p 2 PURCHASER shall not modify pCold Vector DNA sequences located between and gene which is one of the cold shock genes At the downstream of the including the CspA 3 UTR and CspA Promoter The adjacent multi cloning site MCS cspA promoter ac operator is inserted so that the expression is strictly is exempt
6. liance with any applicable licensing requirements described on the product web page It is your responsibility to review understand and adhere to any restrictions imposed by such statements v201110Da pCold Vector Set Sa Shipping at 20 C aesa M geas Store at 20 C x 4 tubes 2 Lot No pCold cspA csp4 5 5 UTR translation enhancing element TEE His Factor Xa multi cloning site acoperator pCold TEE His Factor Xa
7. pstream of MCS So NOTICE TO PURCHASER LIMITED LICENSE the recombinant protein can be expressed directly by using ge site for L13a pCold vectors cloning This product is covered by the claims of U S Patent No 5 981 280 6 686 174 and their foreign counterpart patent claims assigned to the UMDNJ Form This product is covered by the claims of U S Patent No 6 479 260 6 897 042 and 10mM Tris HCI pH8 0 their foreign counterpart patent claims MM EA M9 pCold vectors This product is covered by the claims of U S Patent No 6 479 260 and its foreign Chain length counterpart patent claims pColdIDNA 4407 bp pCold II DNA 4 392 bp Note pCold III DNA 4 377 bp This product is for research use only It is not intended for use in pCold IV DNA 4 359 bp therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Purity Takara products may not be resold or transferred modified for resale ceed u or transfer or used to manufacture commercial products without 1 Confirmed to maintain cloning sites by dideoxy sequencing method written approval from TAKARA BIO INC 2 Shown to be cleaved at a single site by restriction enzymes Nde Sac If you require licenses for other use please contact us by phone at Kpn Xho BamH EcoR Hind Ill Sa Pst and Xba 81 77 543 7247 or from our website at www takara bio com Your use of this product is also subject to comp
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