pBAsi-mU6 Pur DNA
Contents
1. v201007Da 077 543 6116 Ee abe TaKaRa Fe 8223490162. pBAsi pBAsi mU6 Pur DNA EcoRV Ca1 EcoRI promoter mouse U6 BamHI Xbal sse83871 Hind Ill __ EcoRV Cal Pur pBAsi mU6 Pur 3 919 bp SV40 promoter 10 mM Tris HCl pH8 0 1mM EDTA OHN 3919bp OnE 1 dideoxy 2 g77H EcoR 1 1 Cg l EcoRV 2 1 Brummelkamp et a 2002 Science 296 550 553 2 Lee etal 2002 Nat Biotech 20 500 505 3 Paddison et a 2002 Genes and Dev 16 948 958 4 Paul et a 2002 Nat Biotech 20 505 508 5 Sui et a 2002 Proc Natl Acad Sci USA 99 5515 5520 6 Kawasaki et al 2003 Nucleic Acids Res 31 700 707 7 Tuschl T etal 2003 Nat Biotech 20 446 44 8 Shen C et al 2003 FEBS Lett 537 111 114
3. 20 C Store at 20 C 2 Lot No pBAsi mU6 Pur DNA RNA polymerase III pol Ill mouse U6 SIRNA BRATS AS EA pol lll RNA DNA siRNA RNAi siRNA RNA Adenovirus Expression Vector Kit Dual Version Ver 2 6170
4. 9 bp SV40 promoter Form 10mM Tris HCl pH8 0 1mM EDTA Preparation Purified by ion exchange column treatment Chain length 3 919 bp Purity 1 Confirmed to maintain promoter and cloning site by dideoxy sequencing method 2 Confirmed to be cleaved at a single site by restriction enzymes BamH and EcoRI and at two sites by restriction enzymes C a and EcoR V Reference 1 Brummelkamp et a 2002 Science 296 550 553 2 Lee et al 2002 Nat Biotech 20 500 505 3 Paddison et a 2002 Genes and Dev 16 948 958 4 Paul et al 2002 Nat Biotech 20 505 508 5 Sui et al 2002 Proc Natl Acad Sci USA 99 5515 5520 6 Kawasaki et al 2003 Nucleic Acids Res 31 700 707 7 Tuschl T etal 2003 Nat Biotech 20 446 44 8 Shen C et al 2003 FEBS Lett 537 111 114 Note This product is for research use only It is not intended for use in therapeutic or diagnostic procedures for humans or animals Also do not use this product as food cosmetic or household item etc Takara products may not be resold or transferred modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please contact us by phone at 81 77 543 7247 or from our website at www takara bio com Produced by TAKARA BIOTECHNOLOGY DALIAN CO LTD v201007Da pBAsi mU6 Pur DNA Code No 3225 Size 20 ug Shipping at
5. pBAsi mU6 Pur DNA Code No 3225 Size 20 ug Shipping at 20 C Store at 20 C 2 years from date of receipt under proper storage conditions Lot No Concentration g l Volume ul Regarding protocol please refer to the product manual supplied with this vector Description pBAsi mU6 Pur DNA is a plasmid vector containing a RNA polymerase III pol Ill promoter mouse U6 for expression of siRNA and a puromycin resistant gene By inserting synthesized oligo DNA sequence for hairpin RNA into the downstream of the pol lll promoter siRNA expression vector is constructed The obtained plasmid is transfected into cells to perform RNAi experiment By selecting puromycin resistant cells siRNA stable expression cells can be established Moreover the region composed of the promoter and hairpin RNA sequence can be excised and be easily put on adenovirus vector plasmid for the construction of siRNA expression recombinant adenovirus Adenovirus Expression Vector Kit Dual Version Ver 2 Cat 6170 can be used for this purpose Several kinds of plasmid vector are available in pBAsi vector series Each carries each defferent promoter and antibiotic resistant gene A customer can choose an appropriate vector depending ona kind of target cell and an experimental purpose Restriction enzyme map EcoRV chal EcoRI promoter mouse U6 BamH Xbal a Sse83871 Hind Ill __ EcoRV C 1 pBAsi mU6 Pur 3 91
Download Pdf Manuals
Related Search