MightyAmp™ DNA Polymerase
Contents
1. 74 30 10 nmol 1U 10U 06 ug A Hind Ill 74 1 DNA 2 10U 0 6 ug supercoiled pBR322 DNA 74 C 1 DNA Polymerase Chain Reaction PCR DNA PCR MightyAmp DNA Polymerase PCR 3 A 1 PCR T Vector 1 DNA PCR 0 5 2kb 2 DNA PCR 4kb2. on v200904Da TaKaRa 7s Tel 077 543 6116 bx 0778431979
3. units Shipping at 20 C Stored at 20 C Lot No MightyAmp DNA Polymerase 2 kb DNA Polymerase PCR PCR GC AT 98 PCR Ong MightyAmp DNA Polymerase 1 25 U ul 200 ul 2x MA Buffer Mg2 dNTP plus 1ml x 5 2 Mg2 2 x 4mM ae 2 X 800 uM each 50mM Tris HC pH8 2 at 4 100mM Nacl 0 1mM EDTA 1mM DIT 0 1 Tween 20 0 1 Nonidet P 40 50 Glycerol DNA
4. MightyAmp DNA Polymerase Code No R070A Size 250 units Shipping at 20 C Stored at 20 C Components MightyAmp DNA polymerase 2 x MightyAmp Buffer Mg2 dNTP plus Lot No Conc 1 25 units ul Volume 200 ul Expiry Date Description MightyAmp DNA Polymerase is a novel DNA polymerase developed to pursue utmost reactivity for amplification of short length target DNA S about 2 kb especially It is able to amplify a wide range of target DNAs by the polymerase regardless of GC rich or AT rich using crude biological extracts which may contain many inhibitory materials in PCR reaction as templates In addition it is able to amplify excellently a target DNA using a small amount of template by this polymerase This polymerase is a hot start DNA polymerase utilizing a monoclonal anti body suppressible its polymerase activity up to 98 C Storage Buffer 50mM Tris HCl pH8 2 at 4 C 100mM NaCl 0 1mM EDTA 1mM DIT 0 1 Tween 20 0 1 Nonidet P 40 50 Glycerol Unit definition One unit is the amount of the enzyme that will incorpo rate 10 nmol of dNTP into acid insoluble products in 30 minutes at 74 C with activated salmon sperm DNAas the template primer Purity Nicking endonuclease and exonuclease activity were not de tected after incubation of 0 6 u g of supercoiled pBR322 DNA 0 6 ug of A Hind III digest with 10 units of this enzyme for 1 hour at 74 C Application DNA amplifi
5. cation by Polymerase Chain Reaction PCR PCR products As most PCR products amplified with MightyAmp DNA Polymerase have one A added at 3 terminus the obtained PCR product can be directly used for cloning into T vector Also it is possible to clone the product in blunt end vectors after blunting and phosphorylation of the end PCR test 1 Good enzyme performance was confirmed by robust single fragment DNA amplification using human genomic DNA as a template amplified fragments 0 5 2 kb 2 Good enzyme peformance was confirmed by robust single fragment DNA amplification using mouse genomic DNA as a template amplified fragments 4 kb Supplied 2 x MightyAmp Buffer Mg2 dNTP plus Size 1 ml vial X 5 Mg2 concentration 2 X 4mM dNTP concentration 2 X 800 u M each Storage 20 C Regarding general reaction mixture and PCR coditions TM please refer to the product manual supplied with MightyAmp DNA Polymerase NOTICE TO PURCHASER LIMITED LICENSE P1 PCR Notice Use of this product is covered by one or more of the following US patents and corre sponding patent claims outside the US 5 079 352 5 789 224 5 618 711 6 127 155 and claims outside the US corresponding to US Patent No 4 889 818 The purchase of this product includes a limited non transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser s own internal research No right und
6. er any other patent claim such as the patented 5 Nuclease Process claims in US Patents Nos 5 210 015 and 5 487 972 no right to perform an patented method and no right to perform commercial services of any kind includ ing without limitation reporting the results of purchaser s activities for a fee or other commercial consideration is conveyed expressly by implication or by estoppel This product is for research use only Diagnostic uses under Roche patents require a sepa rate license from Roche Further information on purchasing licenses may be obtained by contacting the Director of Licensing Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA L15 Hot Start PCR Licensed under U S Patent No 5 338 671 and 5 587 287 and corresponding patents in other countries Note This product is intended to be used for research purpose only They are not to be used for drug or diagnostic purposes nor are they intended for human use They shall not to be used products as food cosmetics or utensils etc Takara products may not be resold or transfered modified for resale or transfer or used to manufacture commercial products without written approval from TAKARA BIO INC If you require licenses for other use please call at 81 77 543 7247 or contact from our website at www takara bio com Produced by TAKARA BIOTECHNOLOGY DALIAN CO LTD v200904Da MightyAmp DNA Polymerase Code No R070A Size 250
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