FSQ301 - 東洋紡ライフサイエンス事業部
Contents
1. gDNA DNA gDNA Remover RNA gDNA RNA gDNA cDNA PCR PCR PCR THUNDERBIRD qPCR Mix Realtime PCR Master Mix 7 1 DNA cDNA DNA 20 DNA gDNA cDNA2. 4x DN Master Mix 220ul gDNA Remover 4 4ul 2 RNA RNA 65 G 5 7 RNA 4x DN Master Mix 3 DNA DNase 4x DN Master Mix gDNA Remover 2 H RNAtemplate 0 5pg 0 5Hhg Nuclease free Water Total 8 HI 37 C 5 4 3 8 HI 5x RT Master Mix 2 ul Total 10 HI
3. no template control PCR HPLC 7 PCR cDNA PCR cDNA ReverTra Ace qPCR RT Kit 200 FSQ 101 PCR cDNA ReverTra Ace qPCR RT Master Mix 200 CD PCR 1ml x 1 40 QPS 101T PCR THUNDERBIRD Probe qPCR Mix 1 67ml x 3 200 1ml x 1 2 QPS 201T QPS 101 SYBRY Green PCR j THUNDERBIRD SYBR qPCR Mix 1 67ml x 3 200 QPS 201 THUNDERBIRD qPCR M
4. RNA RNA DNA RNA RNA RNase RNA RNA RNA RNase RNA RNA 0 5pg 0 5ug RNA 10ul RNA RNA RNA
5. 5x RT Master Mix I 5x RT Master Mix Il no RT Control cDNA 37 C 15min 50 C 5min 98 C 5min ee 4 C hold ReverTra Ace 4 C 20 C PCR PCR 20 PCR _RNA od 65 C 5min od Nuclease free Water 4xDN Master Mix gdDNA Remover 37C 5min 50C 5min 98C 5min cDNA
6. RNA RNA 65 C 5 37 15 50 C 5 RNA PCR PCR PCR 10 50ng DNA 10ul RNA D NA DNase RNA
7. 2 DNA 20 no RT Control 3 RNA PCR cDNA Oligo dT Random Primer RNA 4 PCR PCR 20 PCR mRNA 1 2 10ul 200 10pl 20 5x RT
8. 2 cDNA lt gt cDNA ReverTra Ace qPCR RT Master Mix with gDNA Remover Code No FSQ 301 DNA ReverTra Ace qPCR RT kit Code No FSQ 101 HeLa total RNA 1pg 10pg 100pg 1ng 10ng 100ng 1ug 20ul FSQ 301 DNA 100ng PCR THUNDERBIRD SYBR qPCR Mix Code No QPS 201 cDNA2u 20ul 10 Target GAPDH 65bD Applied Biosystems 7900HT gt y 1 4846Ln x 34 422 R2 0 999 A FSQ 101 a FSQ 301 Ct N 18 y 1 4838Ln x 34 038 16 RS 0 9991 1 10 100 1000 10000 100000 1000000 RNA Quantity pg DNA FSQ 101 RNA 6 PCR Control RNA RNA RNA
9. 5xRT Master Mix II RT 37 C 15min 5 Appendix 1 gDNA lt gt cDNA ReverTra Ace qPCR RT Master Mix with gDNA Remover Code No FSQ 301 HeLa total RNA 0 5ug 10ul gDNA Remover 4x DN Master Mix gDNA 5x RT Master Mix I 5x RT Master Mix Il no RT Control 4x DN Master Mix 5x RT Master Mix a a ER a gDNA Remover RTase es 1 gDNA Remover RTase PCR THUNDERBIRD SYBR qPCR Mix Code No QPS 201 cDNA2pI 20pl 10 Target ACTB 188bDp Applied Biosystems 7900HT gt mplification Plot 5 000 4 000 3 000 2000 i000 gt Ecke EE EE oo00 1 0 5 10 15 20 25 30 35 40 Cycle Detector sYER gt Plot amp Rn ys cycle Threshold 030 DNA 6
10. Oligo dT Primer dNTPs 5x 5x RT Master Mix Il no RT Control 5x RT Master Mix I ReverTra Ace Nuclease free Water Nuclease free DEPC 1 80 10 2 4x DN Master Mix 5x RT Master Mix 4x DN Master Mix gDNA 3 5x RT Master Mix I ReverTra Ace qPCR RT Master Mix Code No FS
11. Master Mix Il no RT Control 20 C gDNA Remover 20 C 10H 4x DN Master Mix 20 C 440H 5x RT Master Mix I 20 C 400Ll 5x RT Master Mix Il no RT Control 20 C 40Ll Nuclease free Water 20 C 1000ul x2 qDNA Remover DNase 4x DN Master Mix 50 1 4x DN Master Mix RNase inhibitor 4x 4x DN Master Mix 440ul 8 8ul 50 1 gDNA Remover gDNA Remover 4x DN Master Mix 20 C 3 4x DN Master Mix 220ul gDNA Remover 4 4ul 5x RT Master Mix ll ReverTra Ace Random Primer
12. Q 201 5x RT Master Mix 5x RT Master Mix I 42 Nuclease free Water 5 Random Primer Oligo dT Primer Gene Specific Primer 3 37 C 50 C 65 C 98 C Nuclease free Water 200 RNA DEPC Nucleas
13. e free Water DEPC DEPC DEPC PCR Nuclease free Water Total RNA Total RNA Total RNA mRNA 1 2 Total RNA ec 4 1 4x DN Master Mix gDNA Remover 4x DN Master Mix 440ul 8 8ul 50 1 gDNA Remover gDNA Remover 4x DN Master Mix 20 C 3
14. ix 50X ROX reference dye 50ul 1000 QPS 101X5 QPS 201X5 QPS 101 QPS 201 5 http www toyobo co jp bio HPCR 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyobo jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order_lifescience toyobo jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
15. ldeas amp Chemistry ReverTra Ace qPCR RT Master Mix with gDNA Remover Code No FSQ 301 TOYOBO CO LTD Life Science Department OSAKA JAPAN A4633K 1 2 3 4 5 Appendix 6 7 1 ReverTra Ace qPCR RT Master Mix with gDNA Remover DNA gDNA PCR TReverTra Ace PCR cDNA RNA AGPC Acid Guanidium Phenol Chloroform Total RNA gDNA
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