“WAKO BIO WINDOW” No.30(2001.6)
Contents
1. k ES CS m o e iso L1 a rro Ke ENE Ss aH S ko L1 E E E DL mmc o Ou lt E EXE O E a L1 O gt CDD Ort OB ES I L3 a m ES EE UE S E EX a C4 oo r3 T O L o H pg E Au Gei r3 L3 LJ o HL Ei lt equ 2 BD L3 el CO DC Ej z H CQ UE oo E y Se r3 DC o Ed E O a BO emu og a E ES pj a a CO rm L1 E o y D e ko Y o o o z C3 y um a ET G N 51 15 ES 1 15 Ed S E ESI a o o o gg z poc ou o est 3 O cox EB E p Ee E p B n B 0508550 L1 g S Q L3 o o ga E UE L3 L3 O L3 p EH CDe Hi E E E r3 1 al O E 1 EI Q r3 EN LJ CE ex 3 CH r3 Eus a oa a E EI JE E a CD 255 CO o ZC e 1 pt L3 L3 e Q L1 oog CO UB o ois o o o 0 EI o O CG p 5 gt S r3 o m o HEO B Ei r1 o ET o m cr O lt L3 L3 CID O rm COD x r3 EES L2 L3 L3 E C3 ei BOO n c c ri DC o m n Wi V 4 m L3 L3 eo a e E CD A CS OC Oo 20 rn Sch e e e x EEG r 2 tc e y m 7 C3 51 aa aa SR e e H W Wei e 9onnuuunuuunn Guuonnnonnunn PA Zw Yo000000000000000 0000DNa0000000 0 O3000000000000000 AN2. 330 00111 14307 v Amyloid B Proteih Human 1 40 JAntiserum 50u 1 25 000 9 336 00211 14356 v Amyloid B Proteitj Human 34 40 JAntiserum 50u 1 25 0000 333 00221 14357 v Amyloid B Proteitj Human 37 42 JAntiserum 50u 1 25 000 9 330 00231 14359 Amyloid B Proteih Human 1 16 JAntiserum 50u 1 25 0000 B Secretase 0 0 O0 I U D 334 32121 3212 v MOCAc SEVNLDAEFRK Dnp RR NH 1 mg 15 0000 B Secretase 9 339 43781 4378 amp v KTEEISEVN Sta VAEF 1 mg 20 0000 fa PEPTIDE 23 2001 2002 0 UU DETINE 000000000 Bd eiiuuiulllulilltluLl 00000 AA 8 eiuuiilliilillilulLl E D00000000 e unungununn 00000005 HUUU 0000000U5 URL http www peptide co jp WAKO BIO WINDOW E mail biowin wako chemco jp FAX 06 6201 5965 jipgagaggguaugugsuau uuuuu utu O Wako UOUUDUDUUDUOUDUDUDUDUDUDUDUOUDUDUUDUOUDUUI N SNa JUUDUUDUDUDUUDUUDUUDUUDUDUUDUDUUDUUDUUU y UDUDOUUUDUDUDDUUUDUDUDUDDUUUI CHER N DOBO DO DO OO DD ODO OO DD DODo0o0o00L0 is T pUUdgutuluutlttltlu CH ninpi CuHvN NaO S 264 32 DO No 205 15284 WAKO BIO WINDOW No 30 2001 5 DUDDUDUDUDUUDUDUIUU O Wako A OUUU OUOU L ES AAN 1 puupgadguutt ebpePvbrr uttuttlutluttltl 000000 DADO OD ODO DO DO ODO D OD D
3. UUUUDUUUUuuuuuu NunnanadgagngaddnadgbB db B u kepp gt BBpwaunnugagnnggnpnudganmnadgu AA ml P lt Q lt Q 2 a E O a Y a O a Y a Y a Y a O a YA O O a Y a OA a Y a O O O a E WAKO BIO W eld 00D 000000000000 eilllilillilitutliLuttLittLul eililiuiuilliuiuuitutiubliuiiiuttutlitittitiiliil DU uuiulliiilt e Versatainer Y DODBa do Do 000000000000 ICNJI D U B D 000060 I Versatainer Versatainer 0 0 000 HHHP DO DO O HPI HT HI HHH ODO PPU BHI HI D I E Bee H T W KS El Ke J m Ci a e x P 32 Nucleotides Sp Act gt 3000 Ci mmol gt 111 TBq mmol 0000 ATP Sp Act 24000 Ci mmol 2148 TBo mrrol Stabilized aqueous solution i j 19 3 925MBq 25 Ci 12000 ATP y PL 185MBq 0 5001 Ci 16000 Y eA denosine 5 1 riphosphat y PPD 38101X 2x9 25MB4 2x25QCip 18000 T etractriethylammonium Salt 3 MBg Dm 21000 10 mCi mi 370M Bq mi 2x18 5M BA 2x5001Ci 23000 9 3 925MBq 25QiCi 14000 9 dATH a PD 185MBq 500 Ci 18000 eDeoxyadenosine 5 T riphosphat a P 39010X 2x9 25M BA 2x2501 CI 20000 T etratriethylammonium Salt 37MBq UD 1mCig 23000 e10 mCi m 370M Bq mi 2x18 5M BA 2x5001Ci 25000 E 925MBq 0 250 Ci 14000 9 dCTPr a P 185MBg 5001Cig 18000 9 eDeoxycytidine 5 T riphosphat a P 39011X 29 25M BA 2x25
4. 0000000 bMSOm mnn Trifluoroacetate Form neral DO ADOOD DO DD o 240900000 ll O0 O0 104 MI LIU UD UD ugianammauauguagumautgtutddtluttu 200 ugar TEFAL ROU IDOUUUUAR 00024 00000000 0000000000 1 400 POD 00D 0000000000000 UU 4300000000000 0000000 DADO do Bang go o good 000000 lID TFAUJDDDUDDDDDDDDUI DODOD ODO DO DD DO 0000 uuuiuuiuiiLttuL itLubltltlilultll i d an Aa v m AP Be ul Af 1 40 1 TFAL Af 1 40 HCI Y O O O AB 1 4010 HCl 1 AB 1 4010 nn a dg d 0 0 7 UU Dodd 800 00000000000 Af1 1 4000 TFAO IURHCU 00 254 MT O D E U 7 7 7 UI U a3ii ugdag auuduarn tt utut MAP 21 LI LDL U L DUDU 2 336 43791 4379 v Amyloid B Protein Human 1 40 HCl Form 0 5mg 20 000 9 330 43071 4307 v Amyloid B Protein Human 1 40 TFA Form 0 5mg 18 0000 4 WAKO BIO WINDOW No 30 JUN 2001 pad 000 000 334 43091 4309 Armloidf Protein Human 25 350 0 5 mg 4 0000 338 43491 4349v Amyloidg Proteif Human 1 42 0 5 mg 30 0000 337 43581 43587 Sheet Breaker Peptide Af 5 5 mg 16 000 334 43591 4359 v AmyloidB Proteiftj Human 1 160 0 5 mg 10 0000 336 43671 4367 v Pyr Amyloid B Protei Human 3 42 0 5 mg 30 000 333 43701 43 0 Amyloidg Proteiftj Human 1 430 0 5 mg 35 000 9
5. Wako DOBO DO DO DO DO DDD 0000 00Oo DDUDUDUUUDUDUDUDUDUDUUDUDDDUDUDIDI DOBO DO DO OO DO DO OO DDD D0000 DOUUDUDUDUUUDDUDUIDOUUUDUDUDUI DOBO OD DD ODO O 00 0000000000000 DODdD000 BDO DD ODO U E D00000D00 UIDUDUUUDUDUDOUUDUDOUDUDUDOUUDUDUD DDUOUUUDUDUDDUUUUDUDUDOUUUDUDUDUD 043 28191 1 4 200 S SS 0000 e TNT 0000000000000000000000000 000000000000000 No 00000 oos 00 xsi o uguguiuilumuug O E E E E Baur H Neumann HG Carcinogenesis 187V 19800 01717080 Azoxymethane Dn 100mg 13000 223 31 ODO OD DO 00000000 ugna g ugasmympaagoggagngugapnudpuu CH N N CH BL HIH L 26 G DU L L 0 D D Pizzi C Pignata S et al Int J Exp Path 75 30B 1994 CHANOT 74 08 ROOH DER HHH EE BE ss 549 00281 100mg 3 5000 545 00283 500mg 8 5000 A o UU lg 15 000 549 00286 5g 45 0000 HE E E E E E Streptomyces achromogenes DOBO OA O DO DO DD O DO ODO 0D 00000050 OHCH OH uUgalbuliuuttltutlt j A 00000000000000000000 pH450 005M v N N 0000000 508 7 D Oman 0000000 H NCH 0000002100000 EH E E D Rakieten N Rakieten ML Nadkarni MMV Cancer Chemother Rep 2991 1963 CsHis5N5O 265 22 1000006 No 000000 E EE 015 10681 3500 01310682 Alloxan M onohydrate BE
6. 086 00681 lg 1 4006 DOO0D0O000D0000 0000 A L EE 2 309 121 00862 25 4 500 gUDUDDUDUDUUDUDU gt T G RA 0000000000000000 1 E Phenylalanine 1 30g 161 01302 25g 2 0006 JODobDB DD 0000000 pqperolne 1 009 163 04601 lg 1 3006 JUDDDDUUDDDDDUBUDBUDUDDD L Serine 20 80g 199 00402 25g 4 1009 TD pi EFE EL ELTE EE L D i O Threonine 2 30g 202 01323 5 1 450 5 BirTyosine 1 70g 204 03561 5g 9506 poo 0 000 04d 0000 1 L Valine 2 30g 226 00083 5g 1 3506 JUDO OD UD DDD DUU DU i Adenine 5 009 01211512 259 6 0006 LE ET LT L Guanosine 6 70g 077 01112 25g 6 0006 Thymine 1 709 203 01392 259 4 0006 soooooooonoooon Mei mr EE 0DDDBtLD0o0000L000000 1 Succinic Acid 15 00g 190 04332 25g 90060 ammoniumel 7 509 013 02992 259 7506 IIB 0D 0 LO DUDU _SodiumHydroxide 10 809 197 02125 5009 8006 DoOo0oO0000D00D0O0L00000 1 GHPO 105 009 164 04295 5009 1 6009 IA E ELEM HRH HH S Brpoo00 000000 DDDUPLODDODDOODDU emp oroso sa 1308 2 20 A FeSOf 7H O 41 75mg 094 01082 25g 7006 0010000 UU Zo DLO H2504 83i 190 04675 5009 950 50mgll 1 Sictetetetetotetototetototetotetetotetototetotetetotetetotetototetototetotetetotetototetotetetotetetotetetotetetetetetetetotetot 000 No E E 191 12351 250mg 6500 197 12353 Seleno L methionine 185009
7. 13000 02901111 vumm 4 4006 02501113 Benzolalpyrene oomoo 0000005 21000 9 10 Dimethyl 1 2 benzanthracene 1 E E EE E E EE 0000 Misa ds 7 12 Dimethylbeng a Janthracene eer 15 006 039 09691 2 3006 03709692 A Carrageenan ODO D 7500 035 09693 220009 049 00552 ls 4 0006 042 00542 3900 04600545 Dextraf M W 100 000 200 000 EL E HL 336006 14 WAKO BIO WINDOW No 30 JUN 2001 0 00 No Dppnpnnn o o 000000000 147 03421 4Nitroquinoline 1 Oxide somn BE 9 5006 171 00203 Gina m 1500 175 00206 500mL 3 3006 090 01202 Isonicotinohydrazide Hg 3000 039 01276 Carbon T etrachloride ODO 2 15009 204 01202 TS Hr KK 8006 208 01205 e 052 05825 14 Sc SE Cyclophosphamide M onohydrate gogog me a 034 12951 15000 14905882 N Nitrosodimethylamine OD zm 16 000 UD UD HDD OH D HOH 00000000 0000100000 000000009 KTA Jununnmunumununumnunuunnunuumnunnuunununu o 00000000000 O Wako UUguuliiulututLttli DUUDUDUUDUDUDUDUDUDUDUDUDUDUDUU UUUUDUUUDUUDUDUDUDUOUDUOUUUI DU DIIDUDDUDDUUDUDUDUDDDDDUDUDUDDDDU OD DDD ODO OD OD 00000000000 A UBUUBUBUDUDUUDUDUDUDUDUDUDUUD uDUul A OUUUDUDUDOUUDUDUDDOUUUDUDUDUI UUDUDUDUUDUUUDUUUUUDUDUD uDUul 0000000 00 0000000 O Wako go000 00000000 pa0d000 0000001309000 uuuululul Dad gd gg gd 0 od 00d 00 000000000000 II AN uut eilluluuuu uiiliullitLtLitLLtl 30cmx 40cm 0000100000 O 800m x n umuiuuliuuumusui
8. 195 12354 Sol 740009 199 12151 50mg 2 0006 Kol 195 12153 Seleno DL methionine 8 0006 193 12154 48 0006 gt KTA O 1 W A Henarickson J R Horton and D M LeMaster EMBO J 9 166b 1990 2 D M Le Master and F M Richards Biochemistry 24 7263 1988 WAKO BIO WINDOW No 30 JUN 2001 7 genzyme ELISpot Detection System TECHNE DOBO DO DO DO DD DDD DD 000006 DO do god 00000000 0000ELISAJ DOBO DO DO OO DD ODO 00000000 DUDU PVDFIJI II L UIDUDOUUUDUDDUDUUDUDUDUDUUUDUDUDUI DODODODDO DDD 0000 PVDF OO oDDoDoDO DODO DD ODO OO ODO DO 00D 0D0Doo DO DO O O BCIP NBTO DO ADO D 00000000 DODOODDO PVDFIJIUIUIIUIUIUI U UIUUDUDUUUuUupUDUu 0000000000 0 PVOF O OL viliuunungue Ym 00000000 o PVDF 00 mp 0 0111111111111111111111 0 00000000000000 e DDODODOoD eELISA OOOO DO DD 0 00000000000 UU UI umuppnadgdgtutnpPvbprd urn 11 KD DDDDUDDUDUD 1301 1 SDDDDDODDDDDDDDDDDDDODDD 1301 1 12mi 20002 12m 1000000 50m gt BCIP NBT 12m 2000000080000 2ng mi DUU Fe E Li W I a Q z E Human IFN y O PBMC calcium ionomycin PMA 1 D H1 D E D U 0 PBMCO 0 DO D Dag 000000000 UUUJUUUDUUDUUUUUul e 000 No 0000000 a 00000000 509 31111 1403
9. pa Aono G mnmnnnnnnnnnnnnn TN Q9000000000000000000000 quote baccoo000c co 0 0000000000 Dpp0000000 G UJUDDUDUBDUDUDUDUDUUBDUUBUDBUuDJUDUUuDJuupDul O Wako Jjc 000000000000000000000 poo 00000000000 O00000000000000000000000 00 97 0947 T HPLCT puuggagagagaggggggggggggggg Jjc 0000000000000000000000 CH CH DJ aggregetes 0 0 0 0 0000A c D ci 000000000000000000000000 XX 00000 c 00000000000000000 ci ZC Al ci DDDDDDDDD DD D A 20 D 0 0 0 0 Sp St GE C gt H gt ChIN 652 22 No a 00000000 105 00081 55 66 1 etrachloro 1 1 3 3 tetraethylbenzimidazolylcarbocyanine Iodide LU UL ET L 5mg 48 000 KT OUUU 1 CossarizzaA et al Biochem Biophys Res Commun 197 40 1993 2 NuydensR et al J Neurosci Meth 92 153 1999 3 Chanda B and Mathew M K Biochim Biophys Acta 1416 92 1999 poo0o0o000000000 O Wako LE EE EE OH OH D0000000000EcF0000000000 D0001 0MAPpOCO0O0O000000000 H C OH 000000000000000000000000 000000000 11 0000000000000 Hypericum perforatum III 317 00 OH O OH CsoHh kl 504 44 BN po oo fo D DDDDDODI 086 07761 u lmg 100009 Hypericin 1 EL ET F 082 07763 5mg 35000 1 H HI EH PAgostinis et al Biochem Pharmacol 49 1618 1995 KT 00000000000
10. 0 0000000000 0000000 Ys PCROBDO ODO D OB ODO ODO DO DD 000 O E T DNADO0000000000000000000 0000000000000000 DOUE DO BB BOB BD doo oo gt UV VIS 1 1 20000000 PCRODODODO gt 6 3847 0 0 0 000000 e l 340 700nm ell JJ 230 1000nm el jj 400 700nm UIDUIUUUIDIU ei 8 fmol fluorescein well ell 0 0 00000 0 30D ell OOO 04x 10 activity units Alkaline Phosphates JUD UU UI 3400000000000 00000 J LI 000 No Uu ne 506 29891 GENios 176 296 33751 LS Plate Manager 2000 Windows 1700 292 34951 LS Plate Manager 2000 Macintosh 17 00 Y sooooc000000 000000000 000000000000000 0000000000000 00000000000000 MA AS vlt 3w O 46540860591 0000000000010 20 0676203 3741 0 0 4610x030 00000000000 0050 130 28 03B270 857 0 D O ej 0000 0920622 10050 9 el 0 O O O s 082 p85 6381 9 ej 0 0 0 O 0520772 07880 9 ei 0 0 0 s 045 476 2061 9 e 0 0 0 0 0487641 1271 9 e 0 O0 0298 58 2278 e 0 000 0220222 30727 e 0000 011271 02857 0120 5209 p00000000 0120 052 8066 MO 00000 06 6203 2759 03 3270 8124 01 620 5 ot http www wako chem co jp
11. 01 Ci 20000 9 T etracriethylammonium Salt 37MBq ml 230001 10 mCi mi 370M Bq ml 2x185MBd 2x50 Ci 25000 9 3 925MBq 250 Ci 14000 9 185MBq D 5004 Ci 18000 eDeoxyguanosine 5 T riphosphat o P 39012X 2x925MBj 2xX25Eu Ci 20000 T etratriethylammonium Salt 37MBq imci 23000 9 10 mCi mi 370M Bq mi 2x18 5M BA 2x5001Ci 25000 9 3 925MBq 2501CiJ 14000 9 UTPL a uil 185MBg D 5QuCi 18000 eUridine 5 T riphosphat o P 39313X 2x9 25MB4 2x2501 Ci 20000 T etratriethylammonium Salt 37MBq mn 23000 9 10 mCi mi 370M Bol mi 2x18 5M B4 2x5001Ci 25000 9 3 925MBq 254 Ci0 140001 Tile 185MBq SCH 18009 eT hymidine 5 T riphosphat a PQ 39015X 2x9 25M Bd 2x2501Ci 20000 T etratriethylammonium Salt 3 MBg imci 23000 9 10 mCi mi 370M Bol mi 2x18 5M BA 2x5001Ci 25000 9 S 35 Nucleotides Sp Act 21000 Ci mmol 237 TBq mmol T Stabilized aqueous solution dATP a 5S 925MBq 2501 Ci eDeoxyadenosine 5 T riphosphate S 56420H 2x 9 25M BA 2x25Q1Ci e10 mCi mi 370M Bq m 37MBq 10 I WAKO BIO WINDOW No 30 2001 P 33 Nucleotides Sp Act 1000 3000 Ci mmol 37 111 TBo mrrol Stabilized aqueous MII 000000 ne ATP y P 25 0000 eA denosine 5 1 riphosphatef y P 58404 420001 y e10 mCi mi 370M Bq me 58000 dATR o P0 250001 eDeoxyadenosine 5 T
12. 293 56101 Bon EFIE 100r See 299 56103 otel ssay Kapl wako JODODODOOO KM 6 WAKO BIO WINDOW No 30 2001 1 210000800 0 gd 00 0d DO 0 DO 0 dd 00d 00000000000 uummuuuliuuluuluiiiiitibilili Waturel Screnced 00000 80D 000000 0D 0 Dd 0D 00D 0d 0000000000 UUDUUUUUUDUDUIUUUUUUDUDDIUDUUUUU uguagaagaguluuuHuHuudalullutututuiuiutus xtmutt tettlutlutl NMRIJIDDDUDUDDUDUDUDUDDUBUDUDUDUDUUDUDUUXIDDUDUUBGDUUDUUUuUDun DO BDO OO O O DO DO O DO OOO ODO OO DO O ODO Doo ooo ututtisttattii MADU Multi wavelength anomalous Dispersion HUD Do 00000000 uuagautulbluiliil 0 MADI P DAD DO DO DO 000000 UDUUDUOUUDUDUUDUDUUI OOOO O 00D O 0D ib n Dll S DUUDDUDDUDUU IP UIDUDOUUDUDUDUDOUUUDUDUDUUI Apo H IU D LeMaster 10L 2 RR RR RR HUUUDUUDUUDUUDUUDUUDUDUUDDUUDUDUUDUUU DOBO BBB BB 00 II J LeMaster 2 10tg Non ID d 0000000006 0000000000000000 E ro 5 009 010 01042 25g 3 2006 pogo 00000000000 1 rArginin HCI 5 80g 014 04622 25g 1 2006 pg00000800000000D00 _L Asparatic Acid 4 009 013 04832 259 1 0506 Uggdgggagadguguudduu E TfFCystine 0 309 039 05291 5 11009 5 L Glutamic Acid 6 709 070 00502 259 9500 ODO D0D 0000000 1 TFGlutamine 3 309 076 00521 5g 1 0006 JOD0DOD0DD Dc 000000 i Gycne 5 409 07300732 259 9000 00000 L Histidine 0 609
13. 4 Hydrochloride 48000 011 18961 Amyloid B Protein 1421 Hydrochloride OOO Img 52 000 HIHBHHII K TA 1 Iwatsubo T OdakaA Suzuki N et al Neuron 13 48 1991 2 Iwatsubo T Mann D M A OdakaA et al Ann Neurol 37 298 1905 300 040 0040 000000 156200 DOBO OD DO DD ODO ODO OD 0000000 DOBOO DD O DD ODO ODO OD 00000 Ug DODOoDOO Tag god ooo 000000000 UUU UUU UUU UU UUU GU UHUU UHU UUDUDUUDUUUDUDUDUUDUUUUUUDUUDU pugpBaupnBpnupbBagugpiiuButureulttitiDnDubl UUDUDUUDUUUDUDUDUDUUUUUUDUUDU oe JB nlnnnpnnmpmg 54201011 mnm 25008 CAKE GO COO OI DI A DAI ODE O AO NDA ee e ee DADO doo utl pda pg 0000000800 00000000 gABnunuuguppnugpnagnpnaunpnanmagunptu Dgn 0 ogg og DO DO DABO DO 0000000 APP BID o U v d BU D DO D UDUDUUUDUDUDOUUUDUDUDOUUUDUDUDUU UDUDUUUDUDUDOUUUDUDUDOUUUI KTA Wako DU Uudiuetetluttl 9894 LT HPLC O Boc Vali eni gt C NH VaH Je OCH CH E F CaH5NsO F 705 83 i run 190 12321 y Secretase Inhibitor T ypell UU lmg 30000 0 EH HL E EH WOlfe M S er al J Med Chem AL B 19981 K T B WAKO BIO WINDOW No 30 JU PEPTIDE Amyloid B Protein AB 1 40 TFAJ HCIO O O O Amyloid B Protein AB T L1 D
14. D U D D 0000 DO dog Dg o don go odo 000 00004B0 0 poo dp 06 200 Ya 000000000000 DO UABUDDDUDDUDUDUDUDUUDUDUDUDUDDUDDUD DOD ODO Do ABAD Dg o o dodo 00000 Dado gon gogo 0000000000 ACIOO O O ugualiuuuubiiiuiubbliiltiutibitLi tibitLi uguaguugrFrADnugummuaagaguluHitutulul DUUUUDUUUDUIUUDUDUUUDUUUDUID uguHcrmumauggagaaunupPulutull ugaliuiuuliiuililiitiutibilLii tuttLti HBD 0000000 J Neurochem 65 2583 1995 ibid 68 438 1997 0 0 L 0 1567 HHHUHUTHUBUHBBBHUUHDBDUHHIHH 2000 1 0 00000000 0 0 A B 1 400 HCI DO0000000000U0 conformational disease Form 4379 TFA E EH UB BE E HL OE ET E Ll vire uttu AP l IDDUDDUDDDDDDDDDDDDUDDUDDDDUDD uauuuiuuuliuiilililitiutilitiutltL ugurrApnmnmuau auulbuuuiu ulutultluHutut DUUDUUUDUUDUUUUDUUUDUUUID 20 DO000 0 000 0D 00000800000 Methods Enzymol 309 189 1999 JT O0 000000 IDUUUDUUDUUDUUUDUOUDUUDUUUID uguuaduiuiuullutilulititititutLtLililul DOBOADO DO DO BUD DOOLULuLt lt ODO OD DO Do Do AB ugaaiiutuuiitululutl Amyloid Protein Human 1 40 DOdODoODo Doo Bulk Trifluoroacetate Form UUDUDUDUDUDUUUDUIITFA DOAB 1400000000 DMSOD 00000 000000000 4307w00 HPLCO O LI 4307 9 4379 9 uggaeuduH uuHt Amyloidg Protein Human 1 40 Amyloid Protein Human 1 40
15. ELISpot Human IFN y x 76000 506 31121 ELISpot Mouse IFN y 76000 50031141 e openee e 760006 504 31161 1405 ELISpot Mouse TFN a 76 000 GT W No 30 JUN 2001 Ethachinmad jt ign uDnggaupngagagauop UD U D l UUUDUDUUUDUDUDUDUUUUDUUuUUDUupUHnu DNA RNA O 100p 3M Sodium Acetate 3 3H l lt Ethachinmate luz UU OOHUN ei uullllululbLltl 21 2 50 D 00000 eiii unauauuauuuutu a2ecinmutut 000000 eulllulllutltllu 12Kx g l 50 HHDH U UTU e e A A 0 Ethachinmate 0 0 0 0 0 A N E E 0 DNAD O RNAQOOOOOOODOODO DUD D 0 Quocoso oO gd gd nd nd god OAOE ETEO 00 MEROE Y zz Dana 0 T4 DNA Ligase 000000000000000 YN ewe nnnnnnnnnnn Aoo Y anooo00000000000000000 0 AMV Reverse Transcriptasel CONAJO 00000000 D000000 QL00000000DNAO0O0RNADo000000000 T 00040000 Aoo y ng ml n DNA 100base pairs RNA 120 8 DNA Polymerase 0 D ECHT DD D 00D HD DD based I I HHBH HH U 9 26907 0000000000000000 A Aoo 20000000000 BDNA O 10040 0 O O Ethachin mate 1 0 0 D 0 0 00 DNA 0 100 0 0000000 Y sf x O Eu E d doo E ES a L3 E E r3 Eu ES E ES VAN 000000 o0000000000000000000000000000 0 00 E DNAD O O 3004 10 0 U U
16. O Liver DO 00000000000 ubt 1 30800 302 01533 uuagaumulutbuluLuittiuitiuliiLtL tL lkg 116009 308 01611 Liver Porcine JOABO DO DO DO OD OD 0000000000000 4 14 009 O 00 5 8kg0 10 00 1 3kgf D 10 006 301 01721 Lung Bovine Long TIE 0000000000 III 13400 307 01723 Ja ga ooo 0000000000 00 2 5k900 ID 1kg 11 4006 gt 305 01621 Lung Porcine 0 54kg O 10 30 D 88009 304 01571 Pancreas Porcine O Pancreas 0 0000000000 3kg 12 000 1 0 17kg0 0 10 006 303 01541 Spleen Bovine O OO Spleen 0 O0 0000000000000 0000 E 9800 302 01631 Spleen Porcine 1 5k90 0 10 00 0 14k90 0 10 O 10 12 800 301 01581 Stomach Porcine TT Stomach E EIE E ET E E E EI E E ET ET EL Ll 6 112009 000000000000 0 7ko0 0 10 006 30701681 Tendon Bovine I Tendongo0 D 00 D 00000 0 1k900 10 D 2kg 9800 306 01651 Thyroid Porcine Hug ThyroidII duuugdgguidudgduuuu 258 204009 302 01653 50 332009 9 GI DABO ADO DO OO DO DO ODO DD ODO DO D0DO0 0000000 WAKO BIO WINDOW E mail biowinio wako chem co jp FAX 06 6201 5965 WAKO BIO WINDOW No 30 2001 17 20010 00 0000000 Upstate Biotechnology upstate biotechnology UDUOUDUDUDUDUUDUOUDUDUUDUOUI UJUDUDUUDUUuDUUUUDUUUDUDUUUDUuuDUUu DnpnpnpnpnpnpBaDBDBaHrSDDDDODDODODOODU UJUDUDUUDUDUUUUDUUUDUUDUUUUDUupUnu HUHUUDUDUUDUUDDUUDUUDUUUDUUUUBDUU Assay Designs Inc Assay Designs Inc ugnaumluatltBablu csaean C4al D IJ D B D U JUD DD Elcosanoid
17. OD0ODOoDO i TUDUUUIUUUUUUUUUIDUUUUIDUUI en 00000000000000000000D0000 bk 0000 2 0000000000 300p 00000000500 OO 101 15 le uap 3001 OO 100 0 15 A i 30 L 207 0000000 000000001 ODO 0000000000000 06 ono mun e lt I en 000 Guanidine HCI 1M 0 549 ODO DO sp p00000 48000000 Urea 4M 649 PVDEDODOD 10 50 Ammonium Sulfate 1M 1M UD 41 D 100 i KCI 1 Jom 150m NaCI 1M 4M am 3om 150m Mack 0 1M 1M9 60mi 310m Sodium Azide 196 0 2 8 1 0 2m9 m 2004 Im Glycerol 99 Glucose 1M 0 5nM0 Sucrose 50 40 EDTA 0 2M 0 149 IUUDUUDUUDUUDUUUUDUDUDUUD DTT 0 8M 110 000000000PVDFOO0O0O0D0DO0D0O0 PVvDF 1 Mercaptoethanol 1M TUDUUUDUUUIDUUUIDUUUIUUUIUIn Tris 05M 249 p uPvbFrpuuuumsubptatetutltltltluttltLtl HEPES M 0 1M g 6 0nm p000000000000000000 MOPS 1M e 000000000000000 ee SC SE Protein Blot Assay Kit SLS 1999 TUUUUUIDDDUUUUUUUUUUUUUI CTAB 0 0559 00000000000 TE 1e Tween 20 0 196 0 1966 E DUU i Tween 80 0 146 HI Triton X 100 I 0 146 A D i Briji 35 196 Dun O2ug 200g y O O Protein Assay Rapid Kit wako O O DON DU 00 do Protein Blot A ssay Kit wak SCENE dne 29757003 pex CODO BU Msoi 000 DO D 000000000 0000000000 06 1000006
18. Os DODO Dd DDD OOO O DDD OOO DDD DO BHH HU OA DD ODO OO 00000060 uuuuagagdul unagenzuunaugnguun go00000720000000000000000 0000 003000000000 gooo0o0000000000000000000 pooo BODA BBD 00000000 DODO0o 000000 UB uuu ms nn on D DQDDDDUD 305 01741 Adrenal Grand Bovine DO Adrenal Grand 300 01551 Adrenal Grand Porcine O0 DL HH E E Hd HL BE B HE HB EE E DT 7E D 304 01691 Brain Bovine DO Brad JI 0 EHI 0000000000 000000 10 20 400 umagaauuaummuuuabiiuututduiututtuutse O Dd BH U 0 o 3kgf rm 10 inu 0 400000 0000 DO DA Do 0d 0D 00000 IU U po00000000 06 300 01671 Breast Bovine O 00 Breast UD 0000000000000 000 1kg 8600 0000000 0 L kot 10 006 307 01701 Eyeball Bovine O OO Eyebal I B B 00 000000000000 20 23 000 309 01641 Eyeball Porcine iuguubptuiuutiiutdttutttuttLtl AU 17 8006 305 01643 40 28 000 309 01761 Gallbladder Bovine O JU Gallbladder I E O O 6 108009 00000 D 0 3k900 10 00 0 1kg 1 10 00 11 4006 304 01711 Heart Bovine O up Heat DU 000000 0000000000000 114009 301 01601 Heart Porcine uuJgugiad 00000000000 1 7k90 0 1 0 134009 0 0 55kg0 0 10 006 308 01731 Kidney Bovine O Kdne DT 0 LIU ELE E EE EO BE HI DU 2 9 8006 307 01561 Kidney Porcine 0 8kg0 0 10 O0 16kgr 0 10 00000000 00 11400 j 00 LigamentumT I 00000000000 40cm 9800 1000x0 10cm 0 00006 306 01531 Liver Bovine O
19. Steroid goo LU E E UE l DODODO DO O O DO 00000000060 DODODO DO dd O BO 0000000005 uultu BOod 00D 0 0000000000 WAKO BIO WINDOW E mail biowiniwako chem co jp FAX 06 6201 5965 UM O Wako A UUU GUE UU U E A AEO U E O UE GA C DO DO BT E E A E DO DO DD DO DO ODO DDD A OUDUOUUDUOUUDUOUDUDOUDUDUUDUDUUDUDUDUD UIOUUDUOUDUOUDUOUDUUUDUUUDUUUDUU DUUUUDUDUDOUUUDUDUDDUUUI D DD No eamus UDUDOUUDUDUDUDUI pugBugpBgpnunpapngggugmsupelupnpiuiul DUDUDUDUDUDUUUDUUUDUUDUDUDUI 18 046 27743 98 0006 gt s Daidzein from Soybean 0000 EE e 04928073 100mg 298006 ln Glycitin from Soybean 0000 Se 073 04693 100mg 98 000 07004701 10mg 13500 E ame SES 076 04683 100mg 980009 T sme 01018811 6 O A cetylgenistin ugag 150009 01018791 6 0 A cetylglycitin qoop 15000 132 13821 6 0 Malonyldaidzin gt Ug 1mg 150009 13913831 6 0 Malonylglycitin 150009 KTA WAKO BIO WINDOW No 30 JUN 2001 0000000000000000000 ELISAD D D 0 000000 000000 480000 00 OEA 10 130 70 310 oo 00 Kn 00006 0000000 000p gt 000000000000 H gt O00000000 Inteite ABY DO BB ODO 00000000 DD 0000000 DOBODO DO O DD O DD OD BH DDD ODO Doo UH H DO DO DO DO DO DO ODO DO DO DO DODO Doo Di DOB ODO OD ODO DO DO DO OOOO O Doo odo0o 00 UD ecooo0000000000000000000 0000000 evooo0000000 en0000000 miu o
20. U Ethachinmater DADO eyuugggugonbpbbHuBBDDHDOUUHDDDDU 70000 AY PNAD D D 1004 pp00000000 Ethachinmate Ju DD0DDDDDNADDDDDDDDDDDD um DD 9 invia Ethachinmate 0 0 0 0O 300p J 00000 sie Ethachinmate DNAD OU IU 34 0 DH B D D U DT Gy unnn imnvragjnunonnnnunnnnnunnn Ethachinmatej 000000000000000 3M Sodium Acetat Y JA BDO HO 1 BH D B D I O UI D Ethachinmate p0000000000000000 O O O DNAD O 54 0 U Ethachinmate lu 3M Sodium DoOD00000000 Acetate 1 71 DNA 6001 0 Ethachinmate 34 3M Sodium Acetate 19 81 y emer n Lime 00000000 Ethachinmater pp4p48000000000000000 DNAT 00200000080 00000000020 D O O Ethachinmate 00000000000 MA a y ect UHT i i d m o DO OO UO D OD us O Ethachinmate Y 0 0 00 0 00 DNA 4 0 00 ugpBdgdguusuuluttazmmuibptuuuutulutlu ES 000000000000000 900000000 Ethachinmateg 000000000000 Ge A0000 vay LU U U LU LI a e ununauannanuunna nnn q20000000000000 Ethachinmate 0000 f 110000 Y B Pism3200000000000000000000 DS JUDUDDUDUDUUDUDUBDUDUDUDUDUDUDUDUDUDUDUDUDUDUUDUDUUUuu A0000 000 e Ethachinmate 0 I pNAT 0000000000000 e Fthachinmatel 0000000000000000000 21000000 Ethachinmte 000000000000 ez UUDU II UU UUUI VA LU U U LU U LI A WIISST 7008000008000 Safe lockQ 0 D U U d NN 90000000
21. http www wako chem co jp 2001 JUN N 0 30 l L L 0084000000000 0000 pre D D upaed uuu pre WH EIE LE pre v uupnauumnmumiimunt pre 00000 Amyloid B Proteit ABOADOMTFAD 0 HCIO U U 9 pue 2000000 Jer pue 20000 cg gu mg tug gp E EE O O pue SERRE RR IE pre mi 0 genzyme TECHNE ELISpot Detection System 2000 N G 064000000 O pie CND ISOBLUE D T1 B OD pue H EP EH EL BUB ERE U U pad NI 0000 0844000000000 U pue 20000 00000000000 DT e pne 000000 poo e 200 0000 D DABJODO poo e 0000 00000 e BAY 00 9000 2000 Mii a 217 daat mnnn pH e 20000000 D DD D mum EDD 000000 pre 0000000 UBIDO 000 DAssay Desom D D DD DD 200000000 00000 wm0 000000000 00000048000000 D Wako 000000000000 O Wako DOpo0odB A000 0000000000 uggugmugugugagaggugauyugguugugHag DOda gg ono og 0 0000000684000 ec oo0o0o000000000000BA 27 BC 05 I Bo gg oo good 00000000 OOo0o0ARB 40 AB 2000000000000 0 AB 40 AB 4IDOD DO DO DO 0D 00000 uuu DODoDd0 0000 00000001010 20000008400000 140m 0000000000 NoBA21 Jm 2000000840000 1441 dOd0000000 0000 NoBC05 7mi SDUDDDUDDUD DUU 10m 200001980 H IIII 000000000 1m ABCO 0000000000 gagguusuadgllliliBDLgll 10m gt O 90 15
22. m 200000000 50mg AB 42 AB 40 pogo UDUUUI 000060 20000000 200000000200 x20000 ennune SE e 210 ennununne S Le y EST 000000000 0000150 000000 00005000 00000 PBS Tween200 0 050000000000 1504 nnne 500000 20006 000037 1500004 egnnnnune enne 0000000 L9 L9 7 00000 PBS Tween200 0000000000200 00000000000 00000 PBS Tween200 0000198 H DD 0 0 500x2000 00003709 300008 Ag 4000000200000 200x5000 D0000000002000000 o 37 100006 373 100000 ABCO 000 0006 i Le Z AZAR 0 0000 PBS Tween20 ABcnnnannnune 00000PBS Tween20 4 Dap DD 1500000 200x30000 300 0 0 0 0 200 x30100 000201000006 gue DU Dn Ju n junnanmnne 29956701 Amyloid B Protein Immunohistostain Kit LU gH 5010 90 0088 K TA JUN 2001 UgpBagalglililllil UB uult HHH DBUIDBHUHHHHH HUHH HO GOHO IOUUDUOUDUODUDUODUDUUDUDUDUDUDUDUI DUDDDBODDBOP dutttut 1407 0 1421 O HHIH B OUUU BHD HEU E BEEF Li EL EH 000 401421 U L UJUUDDUDUDUDUDUUDUABA2JIIUDUDUDUDUDUDUDA DLL No Wako g 4000000000 po ABpo0 000000800 0000000 IUUDUURUDUDDDDDUDUDUDDUDUUUUUI HHIH DT LH I HII iH THU O00000 A 00000000080 01918761 Amyloid B Proteir 140 T rifluoroacetate 36 006 016 18771 Amyloid B Proteir 1 42 T rifluoroacetate 38000 014 18951 Amyloid B Protein 14
23. o0000000000 0000000000006 00000006 D UUOLUL UJUUDUUDUDUUDUUDUUDUDUUDUUDUDUATIIUUDUUDU Luciferase DOBO BO BB O BBB OOOO 0D00000 000000006 ATPO M9 OO BO D OO DDD OOOO D 0000000000 iu AMP 0xyluciferin P Pi C02 Mg Light Intelrte A8 OT AQ Q1 Intelite AB 00000000000 Q20000000 A1 0 0 0 TNF a Genzyme TECHNE ELISA 2 221 000 Development Kit Mouse TNF a Intelite 48 00 Q3NO0D0DO0O0DO0O00000 0000000 pggdng o 0000000000 InteliteABg poo 4330400000000 0000022000000 Dg go go og Do 0000000000 ol O udguauliuuuiliiiulubiliilibtLiitLlutl ugugaugmumumauaguagmumunuugagauguuuaug OAI UUCMIUUUUDUDUUUDUUUUUUUU HAU U DO Dd OO DO DO O doo 000 00060 Dodo H1 LH Journal of Clinical Ligand Assay 21 358 A4 0H l9G d utastetuttauttt utti CSPD 19981 0980000000000 nteliteAB Y CVO p40000300 1000000200000 O O No n 4 900000000006 302 06871 Intelite AB 1001 10 490 G T 40000001 6 8 9 Jo Seperation Science 2001 0000004 nun BBOOUOUDBOULDLDULD UL L O TECAN JODUDO 00000000000000000000 cell based assay binding assay DNA assay etc y E O UJ gt Q O JUUUDUUUUDUDUUDUDUDUUUUUDUUDuUu N UIDUDUUUDUDUDOUUUDUDUDOUUUDUDUDU A DBgBg ago ono 00 000000000000 l v 384 D0
24. riphosphate a P 58301 420001 e 10 mCi mi 370M Bq mie 58000 SCHEER zm eDeoxycytidine 5 T riphosphate o P0 58430 42000 10 mCi mi 370MBq me 58000 TIME ox 4 eUridine 5 T riphosphate a P J 58313 42000 e10 mCi mi 370M Bq 58000 UMI UIDUDDUUUDDUDUUUUDUDUDOUUDUDUDUDOUUUDUDUDUUUUI UO DH COU OH DD ICN Hl EH D UH BED H D UE 7T DIE UE UUIDUUDUIUUU 111 WA KO BIO WINDOW 1128941 1 H E ELE E E C 000 28 45 E mail biowin wako chem co j p F A X 0120 012895 1 0000 TEL 03 5395 8033 FA X 06 6201 5965 0000 OUUU 0000 D000000000004440000000000 D0000000000000000 ennn DO00000000000000000000000 000 D000000000000000000000000 000 Dn 000 inn HHH UU DHH UUA E LUH LH Du JUJUDUUDUUUUUUUDDDUUUUUuuul 4 0000000000000000000 0000000000 00 No Ey ue 392 01451 000 0 0O00 0 0O00 000000 0000000000 100mix 10 39501441 yodo 0 0001000000000 100mix 10 39901461 0 0 00000 00000 100mix 10 GJ WAKO BIO WIND E E E W y ES n u H O O 1 E l L3 lt 0 L3 Eso al CT Sua lt Q e L3 o 6 L3 O El E SE Oo E E EE E a g E T ue
25. s r3 ES C3 O r3 O Lr o e L1 aa e El a dm d e CC Dnm a ES d NI SH r3 EX E o 2 zm 1 C E Di CT Qu r3 on AE L1 L3 L3 E L1 L1 r3 ra Din bu DO DO E LI Din EE a IF o 0 gt o EL o co ood E E On m a EES L3 L LL L3 ESE 1 1 1 C m Cl o Oo o Lr o EXE L3 r3 L3 E est y es Du c est r3 Ka on El eo O Sa A L3 Zopp L3 ES ES o o0 m Fa Er mm o EES e Ep 28 E Ex 2p A EI a o OD EE r3 N L3 o rn rua E E ES rj 00 o 0 L3 a EJ o Lr ad t EH Be LE Oo Bu rj a O EI oo a p H Dis O CO rn Dr D n CO oo HA o oao o O EE Oo m o o GO E o DD LI L3 1 l c Ld Eu 1 L3 CT EE 1 1 1 1 ie m ca 2 2 12 p H0 E Pl E HH COOC EL 4 E e QS o ao H D LEE It o ODO o o rinm a EE 20 un COOC o o oo EI E pj p CC ti na ES EX s OOO Boo mds t Ee s o 428 E Oc E 1 rc rn rr rc ad L1 CT Ce L3 L3 EE E est CT e L3 LE L3 E L1 L3 est L3 Lr 2 e e e E ud ded EL E EI D D o0 o Dt EE ELE Li geg w es lt e s oy o 5 O E A Sa QQ CT COOC Es a 0 ooa O Sea m Ne E LE EJ EJ EJ L1 COPIE est dis dine A E L1 CO mp SE E jo Np NY e L3 O CIE SS S
26. uuiHtiHlutLtitulitlutl WAKO BIO WINDOW No 30 JUN 2001 15 Wako DABI Peroxidasa PODTI LH E UL ET UU 33 Diaminobenzidine uguguuuudguduaguduagauauuluulluliiil Lil tL 00000 pP7800000000 DOd0b000 000000 0000000000000 DADO DO DO DO DO OO DO DD PTPO DOBO gg BO O DO DO ODO DO 000000000 uggagagulutuiuiuiibABDLDrmtututututututu DODODOODDABOO DO dd Dn Dg 00000000 DO AB ODO DO DO O DO OO 000000005 k No BAJN giu a nna e APRA DAB TRIS Tablet pH7B DA BJ4HCH 1mg M 04327013 pH Img 0 D OUUU 20000 1000000 E 046 27003 100 16 0006 045 22833 100 16500 151 02141 50 5500 50 Rud OPD Tablet 5 Ge 154 02153 gru ugud 9 2006 OPD T ablet 10mg Se 151 02163 gu ugud 9200 158 01671 6000 154 01673 OPD T ablet 13mg 9800 152 01674 2 0001 130 0006 gt 15202171 OPD Tablet OOO K TA IkBa BAY 11 2085 v BAY 11708111 000000000 IK Be 3 D B DU U Ox 20 DO NFKBYOODOO ODO DO DO DO 0D 00000000 SN DU uuiuui iili tLt H C UOUU HO 98941 NMRO CH UuuiullituiutlL C usH NO S 249 33 00 No Em HD HI 000000006 02014331 BASSES 10mg 120009 026 14333 Ud 25mg 2400069 000000 Pierce W et al J Biol Chem 272 21098 1997 ech 16 WAKO BIO WINDOW No 30 2001 0000
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