シカジーニアスRNA抽出試薬 取扱説明書
Contents
1. 6 1 d lt DNA 20C 2 a b b 1 10 RNA 1 2 T103 0022 2 2 1 T541 0048 2 1 T812 0007 2 l 1 RNA a ul b L000 7 RT PCR 1 RNA 100 200 102. RNA 65C 10 RNA 50 RNA DNA RT PCR DNA 7 RNA 80 C 8 6 03 6214 1090 5 06 6231 1672 22 3 092 414 9361 URL http www kanto co jp e mail reag info gms kanto co jp T7407 1 24 2 Cat No 08211 96
3. 1 2 3 65C 6 94 C 3 4 10 000 X amp 5 5 5 DNA 5 RNase 45 ul 6 37 C 15 75 5 RNA 2 8 1 1 3 RNA 2 10 3 RNA 4
4. RNA RNA RNA RT PCR 16S rRNA 1 e a b 1 10 RNA NN i e c d NN DNA RNA 1 1 65C 6 100 94C 3 e SCOD 37 1 RNA 10 000 Xg 5 s 10 000xg 5 5 5 7 Y RNase 45 DNA 5 RNase 45 ei RNA RT 37 15 PCR
5. CE RNA 5 0 ul 5x PrimeScript BuHer 2 0 ul PrmeScript RT Enzyme Mix 1 0 5 u M 1 2 Primer Reverse 2 pmol ul 0 5 100 bp DNA 2 0 Ladder 10 0 1 RNA RNA 42C 15 85 C 5 2 1 PrnmeScript RT Enzyme Mix I PCR 2 0 ul AptaTaq DNA Master 5x conc 4 0 ul Primer Forward 10 pmol ul 1 0 16S rRNA 324 bp Primer Reverse 10 pmol ul 1 0 12 0 McKillip JL et al 1998 4pz Environ Microbiol 64 20 0 4264 4268 PCR 94 30 54C 90 72C 60 X30 3 TBE 30 72C 7 103 0022 2 2 1
6. 03 6214 1090 F 00 2 5 1 06 6222 2796 a 812 0007 2 22 3 092 414 9361 s URL http 0 kanto co jp e mail reag info gms kanto co jp
7. T7407 1 Cat No 08211 96 24 2 RNA 1 RNA RNA RT PCR RNA RNA Cica Geneus RNA Extraction Reagent 1 2 ml X 1 12 0 ml Xx 1 X 1 630 X 1 4 RNA a b RNA DNA c d DNA RT PCR RNA RT PCR 5
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