Qubit® 3.0 Fluorometer アプリケーションノート集(日本語)
Contents
1. Qubit Assay Kit 1 20uL 2 3 gt 2 DNA RNA 15 Qubit Fluorometer Qubit Protein Assay Qubit Fluorometer Qubit Protein Assay T Bio Rad Quick Start Bradford Protein Assay Pierce BCA Protein Assay amp U Pierce Modified Lowry Protein Assay 32. DNA RNA 5 8 Qubit Fluorometer NanoDrop ND 1000 Spectrophotometer 2 Qubit NanoDrop ND 1000 Spectrophotometer UV Qubit Fluorometer NanoDrop ND 1000 Spectrophotometer DNA RN Qubit Fluorometer NanoDrop ND 1000 Spectrophotometer 1 Qubit Fluorometer
3. 500 ng pL 25 ng uL 1 Qubit dsDNA HS Qubit dsDNA BR 1 Qubit 200 400 ng nL 500 ng uL dsDNA HS 25 ng nL 10 20 ng hL 25 ng uL dsDNA BR 25 ng uL RNA BR 25 ng hL RNA HS 25 ng L GlycoBlue 50 ng uL 50 500 ng uL dsDNA HS 25 ng pL 2 5 ng nL 2 5 25 ng L dsDNA BR 25 ng pL RNA BR 25 ng L RNA HS 5 ng bL 8 20
4. 200 L Qubite EN oa 200 L Qubit 5 1 20 LN 1 20 LN 1 20 LM td B41 Qubit 2 0 Fluorometer Qubit Assay Qubit Qubit Fluorometer mix and read DNA RNA 2 M1 DNA RNA Qubit UV Qubit UV
5. UV DNA RNA DNA RNA A 4 610 3 475 2 730 975 804 RE E Qubit dsDNA HS Assay 704 E NanoDrop ND 1000 DNA H rae a ty 304 Q 20 a Wo 10 0 0 01 0 02 003 0 E7 TONA mab 3 Qubit Fluorometer Qubit dsDNA HS Assay DNA NanoDrop ND 1000 Spectrophotometer Qubit D Qubit NanoDrop Spectrophotometer
6. 10nL 15hL 2 10 Qubit Fluorometer Qubit Protein Assay 4 H
7. Qubit Fluorometer Qubit Protein Assay 200 L Qubite 0 oN 200 L eee Qubite 1 20 LN 1 20 LN 1 20 LM 1 Qubit Fluorometer Qubit Protein Assay Qubit Qubit Qubit Fluorometer DNA RNA Qubit Assay Kit
8. H Qubit Protein Assay 12 Qubit dsDNA DNA E YE DNA dsDNA Qubit Fluorometer 2 Qubit dsDNA HS for High Sensitivity Assay Q32851 Qubit dsDNA BR for Broad Range Assay Q32850 1 dsDNA DNA ssDNA Qubit dsDNA dsDNA Qubit dsDNA HS Assay Kit 1 500 ng mL dsDNA Qubit dsDNA BR Assay Kit 0 01 5ug mL dsDNA H
9. NanoDrop Spectrophotometer 1 Glasel JA 1995 Biotechniques 18 62 63 2 Huberman JA 1995 Biotechniques 18 636 3 Manchester KL 1995 Biotechniques 19 208 210 4 Manchester KL 1996 Biotechniques 20 968 970 5 Singer VL Jones LJ Yue ST et al 1997 Anal Biochem 249 228 238 6 Jones LJ Yue ST Cheung CY et al 1998 Anal Biochem 265 368 374 7 LePecq JB 1966 Anal Biochem 17 100 107 8 Kapuscinski J 1995 Biotech Histochem 70 220 233 Qubit Fluorometer Qubit DNA RNA GlycoBlue Glycogen 4 Ibe 8 38 F D HB a 10814010 Qubit RNA BR Qubit RNA HS 4 GlycoBlue A 596 AM9515
10. 1 Bradford MM 1976 Anal Biochem 72 248 254 02 03 04 05 Protein mg mL Concentration determined by assay mg mL 0 8 0 7 4 0 6 4 0 5 4 04 4 0 3 4 0 2 4 0 1 4 0 1 1 Pierce Modified Lowry Protein Assay BSA A Lysozyme HB Histone X lt Bovine pituitary extract 2 Smith PK Krohn RI Hermanson GT et al 1985 Anal Biochem 150 76 85 3 Lowry OH Rosebrough NJ Farr AL et al 1951 J Biol Chem 193 265 275 8 0 1 0 2 0 3 0 4 0 5 Protein mg mL H Qubit Protein Assay 3 A D 3 Qubit Protein Assay Kit BSA Qubit Fluorometer
11. dsDNA DNA 3 fe aH ssDNA dsDNA Qubit Fluorometer DNA ug mL Qubit Fluorometer DNA ug mL 0 10 0 08 0 06 0 04 0 02 0 9 0 8 0 7 0 6 0 5 0 4 0 3 0 2 0 1 E long ssDNA 0 05 g mL dsDNA 0 05 0 05 0 05 0 15 0 05 0 50 dsDNAl long ssDNA yg mL 0 05 0 I oligo DNA E tong ssDNA 0 5 pg mL dsDNA 05 0 05 05 05 15 0 5 5 0 dsDNA long ss or oligo DNA ug mL Qubit Fluorometer DNA ug mL Qubit FLuorometer DNA ug mL I oligo DNA 0 1 g mL dsDNA 0 1 0 0 1 0 1 dsDNAI oligo DNA g mL 0 1 0 3 0 1 1 0 E oligo DNA long ssDNA 5 ug mL dsDNA 5 0 5 5 5 15 5 50 dsDNA long ss or oligo DNA ug mL 3 Qubit Fluorometer Qubit dsDNA BR Assay DNA DNA ssDNA dsDNA 0 1X 3X 10X DNA ssDNA dsDNA 2
12. 20 10uL Qubit Qubit dsDNA HS 1 500 ng mL DNA 50 40 30 20 10 0 GlycoBlue Be Qubite Fluorometer ng mL 1 25ng uL GlycoBlue DNA DNA Qubit dsDNA HS DNA 50ng mL Qubit dsDNA HS 10uL 15nL 3 Qubit dsDNA BR 0 01 5ug mL DNA 0 45 0 40 0 35 0 30 0 25 0 20 0 15 0 10 0 05 Qubit Fluorometer g mL GlycoBlue at 3 25nmg uL
13. 10L 15hL 3 500 400 300 200 100 0 GUycoBlue Qubit Fluorometer ng mL 8 25 ng hL GlycoBlue RNA RNA Qubit RNA HS RNA 500 ng mL Qubit RNA _ HS 25ng uL GlycoBlue RNA 596 10uL 15nL 3 10uL 15nL 3 Qubit RNA HS 25 500 ng mL RNA a T E 45 gE 500
14. 500 ngL GlycoBlue 25 ng uL Qubit dsDNA BR Qubit GlycoBlue Qubit dsDNA HS Qubit RNA BR 3 Qubit RNA HS GlycoBlue 100 ng uL 5 ng uL Qubit DNA RNA DNA RNA Qubit DNA RNA GlycoBlue GlycoBlue
15. 10nL 15uL 25 500 ng mL RNA 50 T E D amp 40 w a 14 30 1J 5 20 3 x 10 g 5 8 0 GlycoBlue 7 25 ng uL GlycoBlue RNA RNA Qubit RNA HS RNA 50ng rmL Qubit RNA HS 596 E 25ng uL GlycoBlue cas ep adieu Qubit Fluorometer Cg mL GlycoBlue 6 25 ngL GUycoBlue RNA RNA Qubit RNA BR RNA 5lg mL Qubit RNA BR
16. dsDNA BR Assay DNA ssDNA dsDNA 3 dsDNA R dsDNA 1096 1 1 Qubit dsDNA HS BR Assay ssDNA ssDNA Qubit dsDNA HS Assay Qubit dsDNA BR Assay 18 mer ssDNA 1 1 ssDNA dsDNA 1 1 ssDNA dsDNA M13mp18 R ssDNA 1 1 ssDNA dsDNA 400 ng mL 1 1 ssDNA dsDNA ssDNA dsDNA 1196 dsDNA 400 ng mL ssDNA dsDNA BR Assay dsDNA HS Assay 15 Ordering information Qubit 3 0 Fluorometer Qubit 3 0 Quantitation Starter Kit Qubit Fluorometer
17. 0 05ug mL A 0 1ng mL B 0 5hg mL C 0 5hg mL D dsDNA 1 1 dsDNA DNA 1196 3 7 BB ve Qubit dsDNA BR Assay 10 mM Tris 1 mM EDTA pH 7 5 buffer dsDNA ssDNA dsDNA dsDNA 1096 dsDNA 0 50ug mL dsDNA 0 50g mL ssDNA dsDNA BR Assay dsDNA 0 52ug mL dsDNA HS Assay dsDNA ssDNA dsDNA dsDNA 8096 400 ng mL dsDNA CTR ssDNA
18. 140 E Qubit dsDNA BR Assay E Qubit RNA BR Assay NanoDrop ND 1000 DNA 100 E UV DNA 1204 l a D c 804 m K 604 PX bx glk 404 204 0 10 0 10 5 10 10 10 20 10 30 10 40 10 50 10 75 10 100 DNA RNA ng uL 2 Qubit Assay UV Qubit dsDNA BR Gubit RNA BR A DNA 10ng pL E coli RNA 0 100 ng pL 3 Qubit Fluorometer NanoDrop ND 1000 Spectrophotometer 3 PerkinElmer Lambda 35 Spectrophotometer 1 Qubit DNA RNA DNA RNA DNA RNA Perkin Elmer Lambda 35 Spectrophotometer 260nm 1 0
19. GUycoBlue DNA DNA Qubit dsDNA BR DNA 0 5hg mL Qubit dsDNA BR 10nL 15nL 2 3 T 2 500 oi oD 400 1G 44 300 1J 3 E 200 5 100 amp 8 0 T T GlycoBlue 2 25ng uL GlycoBlue DNA DNA Qubit dsDNA HS DNA 500ng mL QubitedsDNA HS 10nL 15uL 3 i 4 5 4 0 3 5 3 0 2 5 2 0 1 5 1 0 0 5 0 T T GlycoBlue
20. 2 Bradford Coomassie Brilliant Blue 1 HM 2B BCA E 2 Protein Assay 0 025 mg mL Qubit Pro 2A ein Assay SDS gt 0 01 Tween 20 Triton X 100 1 25 25ug mL 0 25 5hg 2C
21. 2 109 6 ssDNA dsDNA ssDNA dsDNA 1096 A 5004 Qubit DNA HS Assay WE oligo DNA 5 400 4 A long ssDNA o dsDNA cf 5 3004 fae e amp 2004 af w E 104 my 0 0 100 200 300 400 500 Cg mL 2 ssDNA DUT Qubit 18 mer M13 5 TGTAAAACGACGGCCAGT 3 M13mp18 A ssDNA 7 249 bases New England Biolabs N4040S ssDNA A DNA Invitrogen A DNA 25250010 Qubit dsDNA BR Assay Kit Q32850 Qubit dsDNA HS Assay Kit Q32851 B Qubit DNA BR Assay a i VE oligo DNA Do F long ssDNA 22 ER A dsDNA oF E3 4 353Z
22. DNA ng mL Qubit Fluorometer DNA ng mL 5 0 5 5 5 15 5 50 50 0 50 50 50 150 50 500 dsDNA long ss or oligo DNA ng mL dsDNA long ss or oligo DNA ng mL 0 600 500 ng mL dsDNA oligo DNA HB long ssDNA 500 0 500 500 500 1 500 500 5 000 dsDNA long ss or oligo DNA ng mL 500 400 4 300 200 4 Qubit FluorometerG DNA ng mL 100 2 Qubit Fluorometer Qubit dsDNA HS Assay DNA DNA ssDNA dsDNA 0 1X 3X 10X DNA ssDNA dsDNA 2 5 ng mL A 50 ng mL B 500 ng mL C dsDNA Qubit dsDNA HS Assay 10 mM Tris 1 mM EDTA pH 7 5 buffer dsDNA 13 14 Qubit dsDNA BR Assay Kit dsDNA ssDNA Qubit dsDNA BR Assay DNA dsDNA 1 1
23. UV Qubit dsDNA HS Assay Kit Qubit Fluorometer DNA 10 pg uL 1296 3A NanoDrop Spectrophotometer 46 10 ng uL DNA Qubit Fluorometer 196 NanoDrop Spectrophotometer 596 NanoDrop Spectrophotometer 2ng uL Qubit Fluorometer DNA 0 5 ng pL 10 9 6 CV 196 3B NanoDrop Spectrophotometer DNA 4ng uL 9 CV Qubit Fluoromete
24. Qubit Assay Qubit dsDNA HS assay 10 pg uL 100 ng pL Qubit dsDNA BR assay 100 pg uL 1ng uL Qubit ssDNA assay 50 pg uL 200 ng L Qubit RNA assay 250 pg yL 100 ng uL Qubit RNA BR assay 1 ng nL 1pg pL Qubit protein assay 12 5ug mL 5 mg mL Qubit Qubit Qubit Fluorometer DNA RNA Qubit Assay Kit 1 Qubit Assay Kit 1 20uL
25. Qubit Fluorometer Cg mL 4 25ng uL GlycoBlue DNA DNA Qubit dsDNA BR DNA 5Hg mL Qubit dsDNA BR 10nL 15uL 3 Qubit RNA BR 0 1 5ug mL RNA Qubit Fluorometer Cg mL 0 5 0 4 0 3 0 2 0 1 01 GlycoBlue BE 5 25 ng uL GlycoBlue RNA RNA Qubit RNA BR RNA 0 5hg mL Qubit RNA BR 3 Qubit RNA HS
26. dsDNA HS assay 100 assays 1 dsDNA BR assay 100 assays 1 RNAHS assay 100 assays 1 protein assay 100 assays 1 Qubit assay tubes 500 1 Qubit 3 0 NGS Starter Kit Qubit Fluorometer 1 dsDNA HS assay 500 assays 1 Qubit assay tubes 500 1 Qubit dsDNA BR Assay Kit 2 1000 ng Qubit dsDNA BR Assay Kit 2 1000 ng Qubit dsDNA HS Assay Kit 0 2 100 ng Qubit dsDNA HS Assay Kit 0 2 100 ng Qubit ssDNA Assay Kit 1 200 ng Qubit RNA BR Assay Kit 20 1000 ng Qubit RNA BR Assay Kit 20 1000 ng Qubit RNA HS Assay Kit 5 100 ng Qubit RNA HS Assay Kit 5 100 ng Qubit microRNA Assay Kit 1 100ng Qubit microRNA Assay Kit 1 100ng Qubit Protein Assay Kit 0 25 5 ug Qubit Protein Assay Kit 0 25 5 ug Qubit Assay Tubes 100 assays 500 assays 100 assays 500 assays 100 assays 100 assays 500 assays 100 assays 500 assays 100 assays 500 assays 100 assays 500 assays 500 tubes Q33216 195 000 Q33217 215 000 Q33218 210 000 Q32850 10 500 Q32853 35 000 Q32851 10 500 Q32854 35 000 Q10212 10 500 Q10210 10 500 Q10211 35 000 Q32852 10 500 Q32855 35 000 Q32880 10 500 Q32881 35 000 Q33211 10 500 Q33212 35 000 Q32856 6 500 Qub
27. Lowry 3 2D 12 5 5 mg mL CS Qubit A Qubit Protein Assay 0 8 gt 07 4 BSA A Lysozyme Cc 0 6 UH Histone 5 05 1 X Bovine pituitary extract oo TE 044 8 gt g 0 3 Low end of concentration range 5 02 0 0 2 0 15 Ca a 01 0 1 0 05 0 0 0 05 0 0 02 0 06 0 06 0 08 01 0 1 T T T T T 0 0 1 0 2 0 3 0 4 0 5 Protein mg mL B Bio Rad Quick Start Bradford Protein Assay C Pierce BCA Protein Assay 0 8 0 8 5 a7 4 BSA a7 4 BSA v A Lysozyme A Lysozyme ea 0 6 4 MH Histone TA 0 6 Histone GE 054 X Bovine pituitary SE 051 X Bovine pituitary T D s extract 5 extract DE 044 DE 044 E ET S 03 S 034 o 0 2 4 8 0 2 6 0 1 4 ga 0 1 4 6 6 0 0 0 1 0 1 0 0 1 0 2 0 3 0 4 0 5 0 0 1 Protein mg mL B42 Qubit Fluorometer A
28. UV DNA RNA Qubit DNA RNA 2 Qubit dsDNA BR Assay Kit RNA DNA DNA 29 RNA DNA 10 DNA 796 DNA RNA NanoDrop Spectrophotometer UV CDNA 2 3 2 DNA RNA 15 Qubit Fluorometer
29. Qubit dsDNA HS Assay Kit dsDNA ssDNA ssDNA Qubit dsDNA Assay ssDNA dsDNA Qubit dsDNA HS Assay DNA dsDNA 1 1 dsDNA DNA 1196 2 ssDNA dsDNA 1 1 DNA dsDNA ssDNA 89 2A 2B ssDNA dsDNA 1 1 dsDNA ssDNA 219 2C 400 ng mL dsDNA ssDNA Qubit dsDNA BR Assay A B 10 T 5 ng mL dsDNA 100 T 50 ng mL dsDNA I oligo DNA I oligo DNA HB long ssDNA 804 BB long ssDNA Qubit Fluorometer
30. a eS 4 xe su ok 3 E 0 0 2 4 6 8 10 Cg mL 1 Qubit dsDNA HS A BR B Assay Kit DNA ssDNA Qubit dsDNA HS Assay 0 5 500 ng mL Qubit dsDNA BR Assay 0 01 10ug mL 2 DNA Z ADNA A 10 mM Tris 1 mM EDTA pH 7 5 buffer ssDNA DNA DNA ssDNA dsDNA ssDNA Qubit dsDNA HS BR Assay ssDNA 1096 1A 1B Qubit dsDNA ssDNA dsDNA
31. ly bis 2 40 2 a 35 m 400 3 FE go w Uo 25 WJ 5 g a 200 g 15 g 3 3 r 10 100 g g 3S 5 3 3 8 S 0 0 0 25 5 0 2 5 5 GlycoBlue ng L GlycoBlue ng L 9 O 5 ngL GlycoBlue RNA M10 0 5 ng uL GlycoBlue RNA RNA Qubit RNA HS RNA Qubit RNA HS RNA 50ng mL Qubit RNA HS 0 5ngL GlycoBlue W RNA RNA 500 ng mL Qubit RNA HS 596 0 5 ng nL GlycoBlue RNA 10nL 15nL 2 59
32. Invitrogen Qubit 3 0 Fluorometer UV 3 Qubit Fluorometer Qubit DNA RNA GlycoBlue 6 10 Qubit dsDNA DNA 12 Qubit 3 0 Fluorometer technologies BIL N ADE BKC UV Qubit Fluorometer vs NET DNA RNA 260 nm 1 4 UV DNA RNA
33. it Fluorometer www lifetechnologies com qubit www lifetechnologies com TC For Research Use only Not for use in diagnostic procedures 2014 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified Printed in Japan IVN079 A14110B 108 0023 4 2 8 B 0120 477 392 XZ jptechiauifetech com TEL 03 6832 6980 FAX 03 6832 9584 TEL 03 6832 9300 FAX 03 6832 9580 Ej facebook com LifeTechnologiesJapan EJ eifetechJPN www lifetechnologies com technologies AThermo Fisher Scientific Brand
34. r Qubit Assay UV 1 20uL Qubit dsDNA HS Assay Qubit dsDNA BR Assay tT 10 pg uL 1hg hL DNA Qubit RNA Assay Qubit RNA BR Assay 250 pg pL 1ng hL NanoDrop Spectrophotometer 2 ng uL 15pg L 70 E Qubit dsDNA HS Assay z 60 E NanoDrop ND 1000 DNA 50 G gt 30 4 T 204 hh 2 104 Li 01 0 02 R 03 0 1 4 6 10 aN saat 0 01 10 ng uL A DNA 10 Qubit Fluorometer 10 A B A ng L 4 Qubit Assay NanoDrop Spectrophotometer UV
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