pSLG-test, pSLO-test
Contents
1. 0000 006 L 00 00000 0 00 00 000 0000 00 00 00 00 00 000 0 00 00 0 00000 L 00000 0 C 0 0000 000 000 0000 OCCU L 1 2 DNAD 0000 0000 000 0 0 0 3 0 0000000000 MuttiColor Reporter Assay 00 0 0 300000000000000 00000000000 000000002000 300000 100000000 200000 0000000000 00000000000 00000000 000000000000 000000 00000000000000000000 0120 0000 00000000000000 20000 0000 000 00000 00 0000001 30 00000 000000 0000 00000 100 0 0 0000000 00000 0 1 001 0 0 0000 00 0 CHO 4 000 2 g 3000 2 gt 2000 4 lt 100 4 0 RED OR GR RED OR GR RED OR GR 3 Hela NIH3ST3JCHO K10 0000000 0000000 000 00 24 O0 0 00000 00 NIH3T3 CHO K10 0 00 0 00000 00 0 0 000 pSLR O G Sv400 0 0 0000000 0000 00248 0000 0000 00002. 630 00000000000 0000 000 00 Rhagophthalmus 00000 580 00000000 0 0000 000 00 Rhkagophthalmus 0 550 0000 000 E 1000000000 20000000000 000000 0000000000000 00 000 D Lucifeing 0000000000 000000 0 000 0000 0000 0000 00 0 00 0000000 OO L ded 000 000 0000 0 0 0 0 0 O 0000 00000000 100 0 Luc Reporter Assay 100 500 10000 0 10 C C N C 9 H E E E E 00000 00 00 Luc Reporter Assay Systeri O30 00000 0 0000000000 000 0 00 0 0 0000 P E a Ee A 0 000 0 0 0 00 0 0 00 00000 00 0 00 0000 0 00000 00 00 400 800 4501 750 0000 0000 0 00000 0 0 0 00 000 0 0 00000 0000 0 0000 000 00 0 0 60 0 0 0 0000 00 00 00 00000000
3. 00 00006 U 0000 OMultiColor Luc Reporter Assay O 0 0 0 000 0 0 0 0 0000 0 0 0 0 0 0 00 0 0 0 0 000 000000000 000 0 0 0 0 00 SmE 000 0000 00 000 0 20000000 0000000 0 0 Reporter Assay 00000 0000 0 00000 v 0 OOD OUOU 0 0 pSLG test pSLO test Vector 3975 3985 3995 4005 4015 4025 atcagccata ccacatttot SLGOR F primer 4035 4045 4055 4065 4075 4085 cttgctttaa ctgaacctga aacataaaat gaatgcaatt 4095 4105 4115 4125 4135 4145 ottottogtta tgcagcttat aatgogttaca aataaagcaa tagcat 4155 4165 4175 4185 4195 4205 1 tttttcactg cattctaott gtgotttotc 4215 4225 10 20 30 40 aatotatctt atcatotctg ctcgaggtcg acgotatcga Sall Nhe l 50 60 70 80 90 100 g atggctaacg Spe EcoRV EcoRI SLG SLO 110 120 130 140 150 160 0 0 9 666 900 gga cctgggcacc lt CICK SLGO R primer Apal 170 3746 Backgrou
4. 7 207 SLG SLO gene 276 1907 SV40 late poly A signal 1921 2144 SV40 enhancer 2145 2389 2483 3343 background reduction signal 4180 4653 6 pSLG SV40 Cortrol pSLO SV40 Control Vector O OO SV40 Promoter Enhancer Simian virus 40 0 000 000 050 0000 000 0 control 00000 sv40 000 5 40 0000000000 0 05 1 05 7 000 000 5 40 large antigen 0 0000000000060 0000 05 40 late poly A 0000000000 0 l O0 000000 0000000 30 000 2502 0 0000000000000000 000 0 000 0 0 000 0 00 00 000 000 0 0 0 0 0 0 00 12 1 O O 5 40 early polyA signal reduction siyal 10 000000 0000 0 00 0 000 0 0000 000 0 00 0 0 0000 0 00000 00 000 11 5 40 Control Vector 5 40 promoter Background reduction signal SLR PSLR SV40 Luciferase Control 4662 5 40 enhancer 5 40 early 7 207 SLR 276 1916 5 40 late poly A signal 1930 2153 SV40 enhancer 2154 2398 Anp r gene 2492 3352 background reduction signal 4189 4662 7 pSLR SV40 Vector 5 40 Promoter 0 O Enhancer Simian virus 40 60 0000000000000000 0000000000000 000 000 00 0000000 0 contro 0000 0 5 4
5. 1 tttttcactg gtgotttotc 4224 4234 10 20 30 40 ctcgaggtcg taagcttacg cotgctagcg Sall Nhe l 50 60 70 80 90 100 gcagat g Spe Eco RV EcoRI Pstl SLR 110 120 130 140 150 160 agaacat atctggtgtt primer 3755 SLR pSLR test Luciferase 4234 Multiple cloning site 1 81 SLR gene 91 1731 SV40 late poly A signal 1745 1970 Anp r gene 2064 2924 background reduction signal 3761 4234 SLGOR F prin r binding site 3977 4001 SLGO R priner binding site 108 130 Sac Il 1977 Sma 1983 SV40 late poly A siyna 1 200 2500 00000000000 000 00000 12 01 0 5 40 early polyA siyal 1010 00000 000 00 0 00 0 000 0 0000 000 0060 0 0 0 0 0000 0 0 000 00 00 00 0000 0 5 40 Control SV40 Control Vector 5 40 promoter ori Background reduction signal Stable pSLG SV40 cren pSLO SV40 SLO Controli 5 Luciferase 4653bp Orange polyA SV40 enhancer SV40 early
6. O O O 000 000 00 U O O 00000001 00 0 O SLGO R 00 000000000 poez SLR R 0 000000000 Oooo opse 0000 000 0000001 ultiColor SLO Cloning 0 00 12 ultiColor SLR Cloning 0000001 000 a Lysis Buffer O 000 14 Luc Reporter Assay Systeri 030 00000 0 0000000000 0000 000000000 0 000 0000 00 0 00 000 00 0000 0 1000 0000 225010 0 0 0000 000000000000 001000 0000 0 0 0 2500110 0 0000 00 00 0 0560 000 0000 00 0 690 000 0 000 00 0 0 0 0 000 00 0000 000 0 00 URED 000000 00 00000 000 00 0000000000 0 000 00 1000 000000 1 00 00 200 0 000000 2 d 000000000 0000000000000 00000 000 00 10000 Tir 000 00 0000 0 0021000 RED 000 00 20 000 T2 ORD 00 00 20 000 T20 000 00 20 00 0729 RED 00
7. Reporter Assay 0 00 O OMultiColor Reporter Assay 00 0 1 ca A O OOOO 00 0000 0 0 00000 00000 O O O 250011 OOO0O000N0N0 0000 3585439 00 000 0 00 00 0 3272 1954 00000 00000 0 00 0000 0000 0000000 o ultiColor Luc SLG test Vector ultiColor Luc ISLO test Toe 208 ultiColor 0 Vector a ultiColor Luc 51 65 Control Vector e ARS ultiColor Luc 0510 5 Control Vector 2 e ultiColor Luc OSLR SVOO Control Vector 51 Control Vector HSV00 0000 0000000000 VS1 ultiColor Luc Test Vector Set 20u pSLG test pSLO test pSLR test Vector 1 ultiColor Control Vector Set 2011 MCL 52 PSLG 5 40 Control pSLO 5 40 pPSLR 5 40 Control 111 ultiColor Vector Full Set pSLG test pSLO test pSLR 5 m pPSLG SV40 Control pSLO SV40 Control PSL
8. TOYO INK MultiColor Reporter Vector Series Luc Reporter Assay 2 00 000000 00000 Luc Reporter Assay 0 C 4 Voo U U L 5 00 0000000000 9 14 14 15 16 16 00000 2005 10 0 Luc Reporter Assay 5 OOO 0 0000 0 0 0 0 0 0 00 00 0 0 0000 00000 00 00 U Luc Reporter Assay DO 8 050 000000 00 ORNO 00 0 0 00 0 000000000 000000 00010060 1 Reporter Vecto 00 818 00000000 000 0000 10 00 0 00 OREDO OGROD L 000 0 0 00000 000 L 2 0 00000 6 0 Sv40 00 000 000000 0000000000 0 1000 51 5 40 Control 0516 HSV Control Vector Vector SLO 580nm OSLO test Vector OSLO 5 40 Control Vector eve Vector
9. 00 00 0 OMultiColor Luc Reporter Assay O 0 00 00 0 0000000 0000000 000 0 0000 0 0 0 0 0 00 0 0 2000 21000000 000000 000000 0000 2000000000 3000 00000 00 0 00 20 00 0 010 000000 00000 10 0000000 00000 0 0000 0 0 00 0000 0 0000 0 0 00000 0 168000000 000 000 000000000000 2 00000 00000 000 0 00000 000 0 0 0 0 00000 0 CROO 0 00 0 0 000 0 0 0 0 00000 0 0 0 00000 i o 0 0 2000000000000 00000 0000 30000000L 2 00 0000 00 0 00 00 00 0 0200 00 00 000 00000000 0000 00 0 0 0 0 0000 00000 0 0 0000 0 0000 00 00000 0 OOOO 0 00 0 L 0000 000 00 0 0 AU 0 80 0 0 00 00 000 00000000 0000000 000000000 00000 00 000 00000 00000 000 SV40 polyA siad 000 0000 00 00 test Vector SLGO R 05 test Vector SLGOR F primer 00 220 00000 00000 00 0 0000 00 0 000 0000 000000 000 00 090000 00 0 0000 0 0 0000 0000 000000
10. 0 00000000 000 0 05 1 00 05 7 000 000 5 40 larg antigen 0000 000 00 00000 0000 0 00 00 054 late poly A siyal 1 0 000 00 00 0 0 RNA Polymerase I 0 000000 0000000 3000 200 250bp 00000 00000000 000 0 0000 0 0 0 0 0 00 00 00 0 00 0 0 000 0 2 0 0 5 40 early polyA signal 00000 000 Background reduction O0 00000 0000 000 0 00 0 00000 0 00 0 0 0000 000 0 00000 000 0000 0900000000 00 0 00 00000 12 pSLG HSVtk Control Vector HSVtk promoter Background reduction signal SLG pSLG HSVtk 2 4438bp HSVtk promoter 7 234 SLG SLO 304 1935 SV40 late poly A signal 1949 2174 5 2268 3128 background reduction signal 3965 4438 8 pSLG Control Vector 0 00 HSVtk Pronwter Herpes simplex virus thymidine kinase HSVtk 000000 00000 0 00000 0000 0 00 00000 00 00 OSV40 late poly A siyan 00000000000 0 100000000 000 0000 3000 2007 250 0 00 000 0 00 0000 00000 00 0 00 000 0 0 0000 2 5 40 siya 00000000 Background reduction signal 0000000000 13 b
11. 00 3 5 00 0 00000 0 000 0 00 00 00 0 000 0 0 0 00 0 0 0 00000 0020 00000 00000 0000 DNA 2 0 0 0 0 0 00000 20000000 0370 000 000 00000 0000 0 000 MU 00000 0000 00000 DNA 310 0 0000000 000 0 00000 0 00 0 0 00 00 0 6 5 40 polyA 00 0000 00 00 test Vector SLGO R test Vector SLGOR F primer 00 220 00000 00000 00 000 00000 0 0 0 o a 1 9 000 D Luciferin Potassium 0 0 00 00 00900 00 00 00 00 00000 00000 00000 000 00 00000 000000 00 OOL 00 0000 00000 0 0 000 L
12. 00000000 00 0000000 000 00 0 0 0 00 0 0 0000 0000 000 0000 0 FO R G 0 F1 T1r R 10 T1g G F2 T2r R T20 O 20 G ORED OR 0000000000 2 000 00 0 0 0 0 00006 0 0000 0 _ 20 10 29 20 20 1 10 2 E T20A 1r T290A 10 T2r T19 T2r T1r T20 T10o T29 17 20 1 2 2 T29 F1KT1o T1r F2 19 20 2 19 2 2 0 1 20 Tlo T2r 2 20 2 1 1 1 2 19 20 17 20 110 2 1 2 2 92 D00000 00000000N N 0 35854390 0 0 0 15 0 00060 06 0 0 0 0000 0 00000 0000 0 00006 00000 00000000 000000000 9001 17130 0 00000 000 M N N H O A Q N A 2 z 51 O L 16
13. R 5 40 Control OSLG HSV Control Vector 1 O 00L 00000000 00 0 10 0 0 06 000 0 00 00000 1 Luc Reporter Assay 5 Luc Reporter Assay 0000 0000 000 0 0000 00 0000 0 0 200000 0000 0 00 0 00 00 0 0 0 0000 00 0 0000 12 GR OR RED 1 Relative intensity P 01 30000000000000 000 00 000 00000 000 00 0 0000 000 000000 0000 00 0000 0 0 00 0 000 00 000 0000000000000 O L A L 2 00000 0 Luciferases 0 5 5 5 5 5 00000000L D Luciferin Oxyluciferin Red light nax 630 nm Oran light 580 Green light nax 550 nm 0 2 000 00000 1 0000000000000 0000 00000 000000000 pyralis O O 0O 0000 000 0 0 00 0 0 0 0 0 0 000 000 0 0 0 06 000000000 0001000000 00 0 O Phorixothrix hirtus
14. nd reduction signal SLG Stable Luciferase pSLG test 7 or pSLO test SLO Stable Multiple cloning site 1 81 4225bp 516 510 gene 91 1722 5 40 late poly A siqnal 1736 1961 Anp r gene 2055 2915 background reduction signal 3752 4225 SLGOR F prin r binding site 3968 3992 SLGO R priner binding site 105 128 Xba 1 1723 Scal 2359 1729 1980 580 1968 Sma 1 1974 4 pSLG test pSLO test Vector 0 0000 0 MCS 000000000 05050 00000 000000 gsto 0000 SV40 late poly A signaal 1 O0 0000 000000000000 RNA 1 0000000 0000000 3000 2001 250 000 000000 000 000 0 0 000000000000 000 000000 0 0000 000 12 0 O 5 40 polyA siyal 0000000 0 0 Background reduction 0 00000000000000 00 9 test Vector 3984 3994 4004 4014 4024 4034 gt primer 4044 4054 4064 4074 4084 4094 cttgctttaa 4104 4114 4124 4134 4144 4154 ottottogtta tgcagcttat tagcat 4164 4174 4184 4194 4204 4214
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